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Roche restriction enzyme xbai
<t>PFGE</t> profiles of representative S. enterica subsp. enterica strains after digestion with <t>XbaI.</t> As a molecular weight standard (M), S. enterica serovar Braenderup reference strain H9812 was used (lanes 1, 15, and 20). Lanes 2 to 14 represent the 13 different
Restriction Enzyme Xbai, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images

1) Product Images from "Poultry-Associated Salmonella enterica subsp. enterica Serovar 4,12:d:- Reveals High Clonality and a Distinct Pathogenicity Gene Repertoire ▿ Serovar 4,12:d:- Reveals High Clonality and a Distinct Pathogenicity Gene Repertoire ▿ †"

Article Title: Poultry-Associated Salmonella enterica subsp. enterica Serovar 4,12:d:- Reveals High Clonality and a Distinct Pathogenicity Gene Repertoire ▿ Serovar 4,12:d:- Reveals High Clonality and a Distinct Pathogenicity Gene Repertoire ▿ †

Journal:

doi: 10.1128/AEM.02187-08

PFGE profiles of representative S. enterica subsp. enterica strains after digestion with XbaI. As a molecular weight standard (M), S. enterica serovar Braenderup reference strain H9812 was used (lanes 1, 15, and 20). Lanes 2 to 14 represent the 13 different
Figure Legend Snippet: PFGE profiles of representative S. enterica subsp. enterica strains after digestion with XbaI. As a molecular weight standard (M), S. enterica serovar Braenderup reference strain H9812 was used (lanes 1, 15, and 20). Lanes 2 to 14 represent the 13 different

Techniques Used: Molecular Weight

2) Product Images from "Salmonella Cerro isolated over the past twenty years from various sources in the US represent a single predominant Pulsed-Field Gel Electrophoresis type"

Article Title: Salmonella Cerro isolated over the past twenty years from various sources in the US represent a single predominant Pulsed-Field Gel Electrophoresis type

Journal: Veterinary microbiology

doi: 10.1016/j.vetmic.2011.01.026

XbaI pulsed-field gel electrophoresis (PFGE) patterns for 237 Salmonella Cerro isolates included in this study. The isolation year, isolate subcategory, PFGE pattern number and geographic area of isolation are indicating adjacent to individual isolates.
Figure Legend Snippet: XbaI pulsed-field gel electrophoresis (PFGE) patterns for 237 Salmonella Cerro isolates included in this study. The isolation year, isolate subcategory, PFGE pattern number and geographic area of isolation are indicating adjacent to individual isolates.

Techniques Used: Pulsed-Field Gel, Electrophoresis, Isolation

Related Articles

Nucleic Acid Electrophoresis:

Article Title: Antimicrobial resistance and genotypic profiles of Salmonella Saintpaul isolated along beef processing and distribution continuum
Article Snippet: Paragraph title: 2.2.4. Pulsed filed gel electrophoresis (PFGE) procedure ... Agarose-embedded whole genomic DNA of the isolates was digested with the restriction enzyme XbaI ® (60 U) (Roche Diagnostics GmbH, Germany) enzymatic restriction.

Amplification:

Article Title: Multiple-Antibiotic Resistance in Salmonella enterica Serotype Paratyphi B Isolates Collected in France between 2000 and 2003 Is Due Mainly to Strains Harboring Salmonella Genomic Islands 1, 1-B, and 1-C
Article Snippet: Genomic DNA was extracted from brain heart infusion broth cultures as previously described ( ) and was cleaved by the restriction enzyme XbaI (Roche Diagnostics). .. Probe p1-9-like (1,180 bp) and probe QS (721 bp) were generated by PCR amplification using set F and set G, respectively.

Agarose Gel Electrophoresis:

Article Title: The Possible Influence of Non-synonymous Point Mutations within the FimA Adhesin of Non-typhoidal Salmonella (NTS) Isolates in the Process of Host Adaptation
Article Snippet: Briefly, genomic DNA was digested with 50 U of restriction enzyme XbaI (Roche Diagnostics, Indianapolis, IN, USA) for 2 h at 37°C. .. Electrophoresis was carried out using a 1% agarose gel in 0.5 X Tris-borate-EDTA buffer at 14°C in a CHEF Mapper XA System (BioRad Laboratories, Inc., Hercules, CA, USA) with the following conditions: 6 V/cm for 19 h with an initial switch time of 2.16 s and final switch time of 63.8 s. The analysis of the PFGE patterns was performed using BioNumerics software version 5 (Applied Maths, St.-Martens-Latern, Belgium).

Article Title: Antimicrobial resistance and genotypic profiles of Salmonella Saintpaul isolated along beef processing and distribution continuum
Article Snippet: Agarose-embedded whole genomic DNA of the isolates was digested with the restriction enzyme XbaI ® (60 U) (Roche Diagnostics GmbH, Germany) enzymatic restriction. .. A 50–1000 kb Pulse markerTM (Sigma-Aldrich Co, USA), test strains and refernce strain were loaded into 1.2% Pulsed Field Certified Agarose® gel.

Article Title: Salmonella enterica serovar Infantis from Food and Human Infections, Switzerland, 2010–2015: Poultry-Related Multidrug Resistant Clones and an Emerging ESBL Producing Clonal Lineage
Article Snippet: Pulsed-Field Gel Electrophoresis Pulsed-field gel electrophoresis was performed according to the PulseNet protocol of the Centers for Disease Control and Prevention (CDC) , using the restriction enzyme XbaI (Roche, Mannheim, Germany) and Salmonella serovar Braenderup strain H9812 (ATCC BAA 664) as the reference strain. .. Restricted DNA was separated in a 1% agarose gel (BioRad, Cressier, Switzerland) at 12°C for 20 h at 6 V/cm under linear ramp with switch times from 2 to 64 s and 120° included angle using a CHEF-DR III system (BIO-RAD, Munich, Germany).

Article Title: Antimicrobial resistance and genotypic profiles of Salmonella Saintpaul isolated along beef processing and distribution continuum
Article Snippet: Agarose-embedded whole genomic DNA of the isolates was digested with the restriction enzyme XbaI ® (60 U) (Roche Diagnostics GmbH, Germany) enzymatic restriction. .. A 50–1000 kb Pulse markerTM (Sigma-Aldrich Co, USA), test strains and refernce strain were loaded into 1.2% Pulsed Field Certified Agarose® gel.

Southern Blot:

Article Title: Multiple-Antibiotic Resistance in Salmonella enterica Serotype Paratyphi B Isolates Collected in France between 2000 and 2003 Is Due Mainly to Strains Harboring Salmonella Genomic Islands 1, 1-B, and 1-C
Article Snippet: The presence of SGI1 was confirmed by Southern blotting. .. Genomic DNA was extracted from brain heart infusion broth cultures as previously described ( ) and was cleaved by the restriction enzyme XbaI (Roche Diagnostics).

Isolation:

Article Title: Emergence of a new antibiotic resistance mechanism in India, Pakistan, and the UK: a molecular, biological, and epidemiological study
Article Snippet: Plasmids were subsequently isolated and typed on the basis of their origins of replication, as described by Carattoli and colleagues. .. Genomic DNA was prepared in agarose blocks and digested with the restriction enzyme XbaI (Roche Diagnostics, Mannheim, Germany).

Marker:

Article Title: Poultry-Associated Salmonella enterica subsp. enterica Serovar 4,12:d:- Reveals High Clonality and a Distinct Pathogenicity Gene Repertoire ▿ Serovar 4,12:d:- Reveals High Clonality and a Distinct Pathogenicity Gene Repertoire ▿ †
Article Snippet: PFGE using the restriction enzyme XbaI (Roche Diagnostics, Mannheim, Germany) was performed according to the standardized PulseNet Salmonella protocol ( ). .. Strains were assigned to the same PFGE profile type when they showed visually the same fragment sizes between the marker fragment sizes of 1,135 kb and 33 kb.

Spectrophotometry:

Article Title: Molecular Ecology of Listeria monocytogenes: Evidence for a Reservoir in Milking Equipment on a Dairy Farm ▿
Article Snippet: Bacterial cell suspensions for agarose plug preparation were made using an optical density of 1.50 to 1.59 at a wavelength of 610 nm (SmartSpecPlus spectrophotometer; Bio-Rad Laboratories, Hercules CA). .. Salmonella enterica serotype Braenderup (H9812) was used as the reference standard, after digestion with restriction enzyme XbaI (Roche, Indianapolis, IN, or New England BioLabs).

Purification:

Article Title: Multiple-Antibiotic Resistance in Salmonella enterica Serotype Paratyphi B Isolates Collected in France between 2000 and 2003 Is Due Mainly to Strains Harboring Salmonella Genomic Islands 1, 1-B, and 1-C
Article Snippet: Genomic DNA was extracted from brain heart infusion broth cultures as previously described ( ) and was cleaved by the restriction enzyme XbaI (Roche Diagnostics). .. The PCR products were purified with a QIAquick PCR purification kit (QIAGEN, Courtaboeuf, France).

Electrophoresis:

Article Title: The Possible Influence of Non-synonymous Point Mutations within the FimA Adhesin of Non-typhoidal Salmonella (NTS) Isolates in the Process of Host Adaptation
Article Snippet: Briefly, genomic DNA was digested with 50 U of restriction enzyme XbaI (Roche Diagnostics, Indianapolis, IN, USA) for 2 h at 37°C. .. Electrophoresis was carried out using a 1% agarose gel in 0.5 X Tris-borate-EDTA buffer at 14°C in a CHEF Mapper XA System (BioRad Laboratories, Inc., Hercules, CA, USA) with the following conditions: 6 V/cm for 19 h with an initial switch time of 2.16 s and final switch time of 63.8 s. The analysis of the PFGE patterns was performed using BioNumerics software version 5 (Applied Maths, St.-Martens-Latern, Belgium).

Article Title: Salmonella Cerro isolated over the past twenty years from various sources in the US represent a single predominant Pulsed-Field Gel Electrophoresis type
Article Snippet: Paragraph title: 3.2. Pulsed field gel electrophoresis (PFGE) pattern analysis ... PFGE analysis with restriction enzyme XbaI (Roche Molecular Diagnostics, Pleasanton, CA) was performed according to the CDC PulseNet protocol ( ; ).

Polymerase Chain Reaction:

Article Title: Multiple-Antibiotic Resistance in Salmonella enterica Serotype Paratyphi B Isolates Collected in France between 2000 and 2003 Is Due Mainly to Strains Harboring Salmonella Genomic Islands 1, 1-B, and 1-C
Article Snippet: Paragraph title: Detection of Salmonella genomic island 1 by PCR mapping and hybridization. ... Genomic DNA was extracted from brain heart infusion broth cultures as previously described ( ) and was cleaved by the restriction enzyme XbaI (Roche Diagnostics).

Article Title: Emergence of a new antibiotic resistance mechanism in India, Pakistan, and the UK: a molecular, biological, and epidemiological study
Article Snippet: Transconjugants were confirmed to have bla NDM-1 by PCR analysis. .. Genomic DNA was prepared in agarose blocks and digested with the restriction enzyme XbaI (Roche Diagnostics, Mannheim, Germany).

Generated:

Article Title: Multiple-Antibiotic Resistance in Salmonella enterica Serotype Paratyphi B Isolates Collected in France between 2000 and 2003 Is Due Mainly to Strains Harboring Salmonella Genomic Islands 1, 1-B, and 1-C
Article Snippet: Genomic DNA was extracted from brain heart infusion broth cultures as previously described ( ) and was cleaved by the restriction enzyme XbaI (Roche Diagnostics). .. Probe p1-9-like (1,180 bp) and probe QS (721 bp) were generated by PCR amplification using set F and set G, respectively.

Selection:

Article Title: Emergence of a new antibiotic resistance mechanism in India, Pakistan, and the UK: a molecular, biological, and epidemiological study
Article Snippet: Conjugational transfer of antibiotic resistance to the laboratory strain E coli J53 was done on blood agar without selection. .. Genomic DNA was prepared in agarose blocks and digested with the restriction enzyme XbaI (Roche Diagnostics, Mannheim, Germany).

Staining:

Article Title: Salmonella enterica serovar Infantis from Food and Human Infections, Switzerland, 2010–2015: Poultry-Related Multidrug Resistant Clones and an Emerging ESBL Producing Clonal Lineage
Article Snippet: Pulsed-Field Gel Electrophoresis Pulsed-field gel electrophoresis was performed according to the PulseNet protocol of the Centers for Disease Control and Prevention (CDC) , using the restriction enzyme XbaI (Roche, Mannheim, Germany) and Salmonella serovar Braenderup strain H9812 (ATCC BAA 664) as the reference strain. .. Gels were stained with ethidium bromide and visualized under UV light with Gel Doc (BIO-RAD, Munich, Germany).

Sequencing:

Article Title: Emergence of a new antibiotic resistance mechanism in India, Pakistan, and the UK: a molecular, biological, and epidemiological study
Article Snippet: PCR and sequencing were used to identify other resistant genes (bla CMY-4 and bla CTX-M-15 ) carried by the bacterial isolates. .. Genomic DNA was prepared in agarose blocks and digested with the restriction enzyme XbaI (Roche Diagnostics, Mannheim, Germany).

Lysis:

Article Title: Molecular Ecology of Listeria monocytogenes: Evidence for a Reservoir in Milking Equipment on a Dairy Farm ▿
Article Snippet: Lysis of agarose plugs was done in a shaking incubator (Labnet 311 DS; Edison, NJ) for 5 h at 54°C and at 170 rpm. .. Salmonella enterica serotype Braenderup (H9812) was used as the reference standard, after digestion with restriction enzyme XbaI (Roche, Indianapolis, IN, or New England BioLabs).

Software:

Article Title: The Possible Influence of Non-synonymous Point Mutations within the FimA Adhesin of Non-typhoidal Salmonella (NTS) Isolates in the Process of Host Adaptation
Article Snippet: Briefly, genomic DNA was digested with 50 U of restriction enzyme XbaI (Roche Diagnostics, Indianapolis, IN, USA) for 2 h at 37°C. .. Electrophoresis was carried out using a 1% agarose gel in 0.5 X Tris-borate-EDTA buffer at 14°C in a CHEF Mapper XA System (BioRad Laboratories, Inc., Hercules, CA, USA) with the following conditions: 6 V/cm for 19 h with an initial switch time of 2.16 s and final switch time of 63.8 s. The analysis of the PFGE patterns was performed using BioNumerics software version 5 (Applied Maths, St.-Martens-Latern, Belgium).

Article Title: Emergence of a new antibiotic resistance mechanism in India, Pakistan, and the UK: a molecular, biological, and epidemiological study
Article Snippet: Genomic DNA was prepared in agarose blocks and digested with the restriction enzyme XbaI (Roche Diagnostics, Mannheim, Germany). .. DNA fragments were separated by pulsed-field gel electrophoresis (PFGE) on a CHEF-DR III apparatus (Bio-Rad, Hercules, CA, USA) for 20 h at 6 V/cm at 14°C with an initial pulse time of 0·5 s and a final pulse time of 30 s. Dendrograms of strain relatedness were created with BioNumerics software.

Article Title: Salmonella enterica serovar Infantis from Food and Human Infections, Switzerland, 2010–2015: Poultry-Related Multidrug Resistant Clones and an Emerging ESBL Producing Clonal Lineage
Article Snippet: Pulsed-Field Gel Electrophoresis Pulsed-field gel electrophoresis was performed according to the PulseNet protocol of the Centers for Disease Control and Prevention (CDC) , using the restriction enzyme XbaI (Roche, Mannheim, Germany) and Salmonella serovar Braenderup strain H9812 (ATCC BAA 664) as the reference strain. .. GelCompar II software (Applied Maths NV, Sint-Martens-Latem, Belgium) was used for analysis.

Pulsed-Field Gel:

Article Title: The Possible Influence of Non-synonymous Point Mutations within the FimA Adhesin of Non-typhoidal Salmonella (NTS) Isolates in the Process of Host Adaptation
Article Snippet: Paragraph title: Pulsed-field gel electrophoresis (PFGE) ... Briefly, genomic DNA was digested with 50 U of restriction enzyme XbaI (Roche Diagnostics, Indianapolis, IN, USA) for 2 h at 37°C.

Article Title: Emergence of a new antibiotic resistance mechanism in India, Pakistan, and the UK: a molecular, biological, and epidemiological study
Article Snippet: Genomic DNA was prepared in agarose blocks and digested with the restriction enzyme XbaI (Roche Diagnostics, Mannheim, Germany). .. DNA fragments were separated by pulsed-field gel electrophoresis (PFGE) on a CHEF-DR III apparatus (Bio-Rad, Hercules, CA, USA) for 20 h at 6 V/cm at 14°C with an initial pulse time of 0·5 s and a final pulse time of 30 s. Dendrograms of strain relatedness were created with BioNumerics software.

Article Title: Salmonella Cerro isolated over the past twenty years from various sources in the US represent a single predominant Pulsed-Field Gel Electrophoresis type
Article Snippet: Paragraph title: 3.2. Pulsed field gel electrophoresis (PFGE) pattern analysis ... PFGE analysis with restriction enzyme XbaI (Roche Molecular Diagnostics, Pleasanton, CA) was performed according to the CDC PulseNet protocol ( ; ).

Article Title: Salmonella enterica serovar Infantis from Food and Human Infections, Switzerland, 2010–2015: Poultry-Related Multidrug Resistant Clones and an Emerging ESBL Producing Clonal Lineage
Article Snippet: .. Pulsed-Field Gel Electrophoresis Pulsed-field gel electrophoresis was performed according to the PulseNet protocol of the Centers for Disease Control and Prevention (CDC) , using the restriction enzyme XbaI (Roche, Mannheim, Germany) and Salmonella serovar Braenderup strain H9812 (ATCC BAA 664) as the reference strain. .. Restricted DNA was separated in a 1% agarose gel (BioRad, Cressier, Switzerland) at 12°C for 20 h at 6 V/cm under linear ramp with switch times from 2 to 64 s and 120° included angle using a CHEF-DR III system (BIO-RAD, Munich, Germany).

Hybridization:

Article Title: Multiple-Antibiotic Resistance in Salmonella enterica Serotype Paratyphi B Isolates Collected in France between 2000 and 2003 Is Due Mainly to Strains Harboring Salmonella Genomic Islands 1, 1-B, and 1-C
Article Snippet: Paragraph title: Detection of Salmonella genomic island 1 by PCR mapping and hybridization. ... Genomic DNA was extracted from brain heart infusion broth cultures as previously described ( ) and was cleaved by the restriction enzyme XbaI (Roche Diagnostics).

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  • 94
    Roche xbai restriction enzyme
    a The differences in plasmid number from a selection of bacteria isolates. Several isolates contained up to eight plasmids b S1-PFGE and Southern blotting of plasmids carrying <t>NDM-1</t> from clinical isolates. Pulsed-field gel of S1-treated plasmid DNA of selected Enterobacteriaceae from clinical isolates stained with ethidium bromide. The molecular weight marker is S. braenderup H9812 cut with <t>XbaI</t> ( A ). Autodiagram of gel A showing plasmids carrying NDM-1 ( B )
    Xbai Restriction Enzyme, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xbai restriction enzyme/product/Roche
    Average 94 stars, based on 6 article reviews
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    86
    Roche restriction enzymes xbai
    Pulsed-field gel electrophoresis (PFGE) profiles of representative S . Typhimurium, PT Untypable, ASSuT isolates digested with <t>BlnI</t> . Lane 1, H9812 ( S . Braenderup control), lane 2, 03–0407, lane 3, 05–0802, lane 4, 05–0900, lane 5, H9812 ( S . Braenderup control), lane 6, 05–0902, lane 7, 07–0028, lane 8, 07–0060, lane 9, 07–0146, lane 10, H9812 ( S . Braenderup control), lane 11, 07–0174, lane 12, 07–0200, lane 13, 07–0201, lane 14, 07–0204, lane 15, H9812 ( S . Braenderup control). PFGE with both <t>XbaI</t> and BlnI was performed on all isolates with same phenotype as isolate 07–0146. Digestion with BlnI proved more discriminatory showing 07–0146 and 07–0237 to be indistinguishable from each other and different from other isolates in our collection.
    Restriction Enzymes Xbai, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction enzymes xbai/product/Roche
    Average 86 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    restriction enzymes xbai - by Bioz Stars, 2020-04
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    a The differences in plasmid number from a selection of bacteria isolates. Several isolates contained up to eight plasmids b S1-PFGE and Southern blotting of plasmids carrying NDM-1 from clinical isolates. Pulsed-field gel of S1-treated plasmid DNA of selected Enterobacteriaceae from clinical isolates stained with ethidium bromide. The molecular weight marker is S. braenderup H9812 cut with XbaI ( A ). Autodiagram of gel A showing plasmids carrying NDM-1 ( B )

    Journal: European Journal of Clinical Microbiology & Infectious Diseases

    Article Title: Common isolation of New Delhi metallo-beta-lactamase 1-producing Enterobacteriaceae in a large surgical hospital in Vietnam

    doi: 10.1007/s10096-015-2345-6

    Figure Lengend Snippet: a The differences in plasmid number from a selection of bacteria isolates. Several isolates contained up to eight plasmids b S1-PFGE and Southern blotting of plasmids carrying NDM-1 from clinical isolates. Pulsed-field gel of S1-treated plasmid DNA of selected Enterobacteriaceae from clinical isolates stained with ethidium bromide. The molecular weight marker is S. braenderup H9812 cut with XbaI ( A ). Autodiagram of gel A showing plasmids carrying NDM-1 ( B )

    Article Snippet: NDM-1-positive bacterial isolates were genotyped by pulsed-field gel electrophoresis (PFGE), using XbaI restriction enzyme (Roche Diagnostic, Mannheim, Germany) to digest the bacterial genomes in agarose blocks.

    Techniques: Plasmid Preparation, Selection, Southern Blot, Pulsed-Field Gel, Staining, Molecular Weight, Marker

    PFGE profiles of representative S. enterica subsp. enterica strains after digestion with XbaI. As a molecular weight standard (M), S. enterica serovar Braenderup reference strain H9812 was used (lanes 1, 15, and 20). Lanes 2 to 14 represent the 13 different

    Journal:

    Article Title: Poultry-Associated Salmonella enterica subsp. enterica Serovar 4,12:d:- Reveals High Clonality and a Distinct Pathogenicity Gene Repertoire ▿ Serovar 4,12:d:- Reveals High Clonality and a Distinct Pathogenicity Gene Repertoire ▿ †

    doi: 10.1128/AEM.02187-08

    Figure Lengend Snippet: PFGE profiles of representative S. enterica subsp. enterica strains after digestion with XbaI. As a molecular weight standard (M), S. enterica serovar Braenderup reference strain H9812 was used (lanes 1, 15, and 20). Lanes 2 to 14 represent the 13 different

    Article Snippet: PFGE using the restriction enzyme XbaI (Roche Diagnostics, Mannheim, Germany) was performed according to the standardized PulseNet Salmonella protocol ( ).

    Techniques: Molecular Weight

    Pulsed-field gel electrophoresis (PFGE) profiles of representative S . Typhimurium, PT Untypable, ASSuT isolates digested with BlnI . Lane 1, H9812 ( S . Braenderup control), lane 2, 03–0407, lane 3, 05–0802, lane 4, 05–0900, lane 5, H9812 ( S . Braenderup control), lane 6, 05–0902, lane 7, 07–0028, lane 8, 07–0060, lane 9, 07–0146, lane 10, H9812 ( S . Braenderup control), lane 11, 07–0174, lane 12, 07–0200, lane 13, 07–0201, lane 14, 07–0204, lane 15, H9812 ( S . Braenderup control). PFGE with both XbaI and BlnI was performed on all isolates with same phenotype as isolate 07–0146. Digestion with BlnI proved more discriminatory showing 07–0146 and 07–0237 to be indistinguishable from each other and different from other isolates in our collection.

    Journal: BMC Microbiology

    Article Title: Role of subtyping in detecting Salmonella cross contamination in the laboratory

    doi: 10.1186/1471-2180-9-155

    Figure Lengend Snippet: Pulsed-field gel electrophoresis (PFGE) profiles of representative S . Typhimurium, PT Untypable, ASSuT isolates digested with BlnI . Lane 1, H9812 ( S . Braenderup control), lane 2, 03–0407, lane 3, 05–0802, lane 4, 05–0900, lane 5, H9812 ( S . Braenderup control), lane 6, 05–0902, lane 7, 07–0028, lane 8, 07–0060, lane 9, 07–0146, lane 10, H9812 ( S . Braenderup control), lane 11, 07–0174, lane 12, 07–0200, lane 13, 07–0201, lane 14, 07–0204, lane 15, H9812 ( S . Braenderup control). PFGE with both XbaI and BlnI was performed on all isolates with same phenotype as isolate 07–0146. Digestion with BlnI proved more discriminatory showing 07–0146 and 07–0237 to be indistinguishable from each other and different from other isolates in our collection.

    Article Snippet: DNA was digested using restriction enzymes XbaI (Roche, Basel, Switzerland) and BlnI (Sigma -Aldrich, Dorset, England) and DNA fragments were separated using the CHEF Mapper XA (Bio-Rad, California) system.

    Techniques: Pulsed-Field Gel, Electrophoresis

    PFGE findings (A and C) and Southern blot hybridization (B and D) with bla NDM-1 (A and B) and an IS CR1 probe (C and D). The genomic DNA of the index strain ( K. pneumoniae 05-506) was undigested (lane 1) or was digested with S1 nuclease (lane 2), XbaI

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Characterization of a New Metallo-?-Lactamase Gene, blaNDM-1, and a Novel Erythromycin Esterase Gene Carried on a Unique Genetic Structure in Klebsiella pneumoniae Sequence Type 14 from India ▿

    doi: 10.1128/AAC.00774-09

    Figure Lengend Snippet: PFGE findings (A and C) and Southern blot hybridization (B and D) with bla NDM-1 (A and B) and an IS CR1 probe (C and D). The genomic DNA of the index strain ( K. pneumoniae 05-506) was undigested (lane 1) or was digested with S1 nuclease (lane 2), XbaI

    Article Snippet: Genomic DNA was prepared in agarose blocks and digested with the restriction enzymes XbaI and SpeI (Roche Diagnostics, Mannheim, Germany), I- Ceu I (New England Biolabs, Beverly, MA), and S1 nuclease (Invitrogen, Abingdon, United Kingdom).

    Techniques: Southern Blot, Hybridization