restriction enzyme sma  (New England Biolabs)


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    New England Biolabs restriction enzyme sma
    Restriction Enzyme Sma, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    restriction enzyme sma  (New England Biolabs)


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    New England Biolabs restriction enzyme sma
    Restriction Enzyme Sma, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    restriction enzyme sma  (New England Biolabs)


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    New England Biolabs restriction enzyme sma
    Restriction Enzyme Sma, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    restriction enzymes sma  (New England Biolabs)


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    New England Biolabs restriction enzymes sma
    Restriction Enzymes Sma, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    methylation sensitive restriction enzyme sma  (New England Biolabs)


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    New England Biolabs methylation sensitive restriction enzyme sma
    Methylation Sensitive Restriction Enzyme Sma, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    sma i restriction enzyme  (New England Biolabs)


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    New England Biolabs sma i restriction enzyme
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    restriction enzyme sma  (New England Biolabs)


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    New England Biolabs restriction enzyme sma
    Restriction Enzyme Sma, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    restriction enzymes sma  (New England Biolabs)


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    New England Biolabs restriction enzymes sma
    Restriction Enzymes Sma, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    sma i restriction enzyme  (New England Biolabs)


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    New England Biolabs sma i restriction enzyme
    Chloroplast transformation vectors, site‐specific transgene integration and maternal inheritance of laccase and lignin peroxidase genes. (a) Schematic representation of chloroplast trn l/ trn A spacer region of untransformed wild‐type (UT‐WT) tobacco (b) chloroplast vectors of laccase (Lac) and lignin peroxidase (LiP) genes. Solid arrowhead with dotted line represents an <t>expected</t> <t>DNA</t> fragment size of ~4.0 kb from UT‐WT, and ~7.2, ~6.7 and ~6.6 kb from Ao Lac and Pc LiP transplastomic lines after digestion with <t>Sma</t> <t>I</t> restriction enzyme. A ~0.8 kb Bam HI and Bgl II restriction enzyme digested DNA fragment was used as probe for Southern blot analysis. Map is not in scale. (c) Seedlings from a homoplasmic transplastomic line (green, left) and untransformed wild‐type (UT‐WT, bleached white, right) germinated on 500 mg/L spectinomycin plate showing maternal inheritance. (d–f) Confirmation of integration of ligninase genes into the tobacco chloroplast genome. (d) Amplification of ~1.6 kb fragment using 16SrRNA _F (3P)/ aadA _R (3M). Amplification of ~1.1 kb (e) and (f) ~4.2 kb fragments using PpsbA _F (DV9)/ TpsbA _R (DV13) and aadA _F (5P)/ trnA _R (2M) from Cp‐LiP ( Pc LiP) respectively. Lanes M1 and M2 are DNA molecular weight ladders (NEB 1 kb ladder and Invitrogen 1 kb ladder plus, respectively). Lane 1 is negative control. Lane 2 is UT‐WT. Lanes 3 to 8 are six independent Cp‐LiP transplastomic lines.
    Sma I Restriction Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Abatement of microfibre pollution and detoxification of textile dye – Indigo by engineered plant enzymes"

    Article Title: Abatement of microfibre pollution and detoxification of textile dye – Indigo by engineered plant enzymes

    Journal: Plant Biotechnology Journal

    doi: 10.1111/pbi.13942

    Chloroplast transformation vectors, site‐specific transgene integration and maternal inheritance of laccase and lignin peroxidase genes. (a) Schematic representation of chloroplast trn l/ trn A spacer region of untransformed wild‐type (UT‐WT) tobacco (b) chloroplast vectors of laccase (Lac) and lignin peroxidase (LiP) genes. Solid arrowhead with dotted line represents an expected DNA fragment size of ~4.0 kb from UT‐WT, and ~7.2, ~6.7 and ~6.6 kb from Ao Lac and Pc LiP transplastomic lines after digestion with Sma I restriction enzyme. A ~0.8 kb Bam HI and Bgl II restriction enzyme digested DNA fragment was used as probe for Southern blot analysis. Map is not in scale. (c) Seedlings from a homoplasmic transplastomic line (green, left) and untransformed wild‐type (UT‐WT, bleached white, right) germinated on 500 mg/L spectinomycin plate showing maternal inheritance. (d–f) Confirmation of integration of ligninase genes into the tobacco chloroplast genome. (d) Amplification of ~1.6 kb fragment using 16SrRNA _F (3P)/ aadA _R (3M). Amplification of ~1.1 kb (e) and (f) ~4.2 kb fragments using PpsbA _F (DV9)/ TpsbA _R (DV13) and aadA _F (5P)/ trnA _R (2M) from Cp‐LiP ( Pc LiP) respectively. Lanes M1 and M2 are DNA molecular weight ladders (NEB 1 kb ladder and Invitrogen 1 kb ladder plus, respectively). Lane 1 is negative control. Lane 2 is UT‐WT. Lanes 3 to 8 are six independent Cp‐LiP transplastomic lines.
    Figure Legend Snippet: Chloroplast transformation vectors, site‐specific transgene integration and maternal inheritance of laccase and lignin peroxidase genes. (a) Schematic representation of chloroplast trn l/ trn A spacer region of untransformed wild‐type (UT‐WT) tobacco (b) chloroplast vectors of laccase (Lac) and lignin peroxidase (LiP) genes. Solid arrowhead with dotted line represents an expected DNA fragment size of ~4.0 kb from UT‐WT, and ~7.2, ~6.7 and ~6.6 kb from Ao Lac and Pc LiP transplastomic lines after digestion with Sma I restriction enzyme. A ~0.8 kb Bam HI and Bgl II restriction enzyme digested DNA fragment was used as probe for Southern blot analysis. Map is not in scale. (c) Seedlings from a homoplasmic transplastomic line (green, left) and untransformed wild‐type (UT‐WT, bleached white, right) germinated on 500 mg/L spectinomycin plate showing maternal inheritance. (d–f) Confirmation of integration of ligninase genes into the tobacco chloroplast genome. (d) Amplification of ~1.6 kb fragment using 16SrRNA _F (3P)/ aadA _R (3M). Amplification of ~1.1 kb (e) and (f) ~4.2 kb fragments using PpsbA _F (DV9)/ TpsbA _R (DV13) and aadA _F (5P)/ trnA _R (2M) from Cp‐LiP ( Pc LiP) respectively. Lanes M1 and M2 are DNA molecular weight ladders (NEB 1 kb ladder and Invitrogen 1 kb ladder plus, respectively). Lane 1 is negative control. Lane 2 is UT‐WT. Lanes 3 to 8 are six independent Cp‐LiP transplastomic lines.

    Techniques Used: Transformation Assay, Southern Blot, Amplification, Molecular Weight, Negative Control

    Evaluation of homoplasmy of transplastomic Cp‐laccase ( Ao Lac) and Cp‐LiP ( Pc LiP) plants by Southern blot analyses. Southern blot hybridization profile of Sma I restriction enzyme‐digested DNA isolated from leaves of Cp‐laccase, Cp‐LiP transplastomic and untransformed WT plants after hybridization with the probe. Presence of a single ~7.2 kb and ~6.7 kb band in five independent (lanes 1–5) lines of Cp‐laccase (a) and Cp‐LiP (b) transplastomic plants confirms stable integration and homoplasmic status, whereas existence of a single ~4.0 kb is expected WT (lane WT) plant (see Figure for chloroplast vector maps). Membrane after DNA transfer was hybridized with ~0.8 kb Bam HI and Bgl II restriction enzyme‐digested DNA fragment as the probe for Southern blot analysis.
    Figure Legend Snippet: Evaluation of homoplasmy of transplastomic Cp‐laccase ( Ao Lac) and Cp‐LiP ( Pc LiP) plants by Southern blot analyses. Southern blot hybridization profile of Sma I restriction enzyme‐digested DNA isolated from leaves of Cp‐laccase, Cp‐LiP transplastomic and untransformed WT plants after hybridization with the probe. Presence of a single ~7.2 kb and ~6.7 kb band in five independent (lanes 1–5) lines of Cp‐laccase (a) and Cp‐LiP (b) transplastomic plants confirms stable integration and homoplasmic status, whereas existence of a single ~4.0 kb is expected WT (lane WT) plant (see Figure for chloroplast vector maps). Membrane after DNA transfer was hybridized with ~0.8 kb Bam HI and Bgl II restriction enzyme‐digested DNA fragment as the probe for Southern blot analysis.

    Techniques Used: Southern Blot, Hybridization, Isolation, Plasmid Preparation

    restriction enzyme sma  (New England Biolabs)


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    New England Biolabs restriction enzyme sma
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    New England Biolabs restriction enzymes sma
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    New England Biolabs methylation sensitive restriction enzyme sma
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    sma i restriction enzyme  (New England Biolabs)
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    New England Biolabs sma i restriction enzyme
    Sma I Restriction Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    sma i restriction enzymes  (New England Biolabs)
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    New England Biolabs sma i restriction enzymes
    Sma I Restriction Enzymes, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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