Restriction Enzyme Pvuii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 12 article reviews
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1) Product Images from "Modulation of gene expression in U251 glioblastoma cells by binding of mutant p53 R273H to intronic and intergenic sequences"
Article Title: Modulation of gene expression in U251 glioblastoma cells by binding of mutant p53 R273H to intronic and intergenic sequences
Journal: Nucleic Acids Research
Figure Legend Snippet: Recombinant p53 R273H interacts preferentially with ChIP DNA sequences in vitro . ( A–B ) Four selected ChIP DNA sequences were tested for their interaction with p53 R273H using EMSA. For the binding reaction, either PvuII-restriction fragments of empty or insert containing pCRII vector (A), or supercoiled forms of plasmid DNA (B) were used. Increasing amounts of p53 R273H protein (marked by p53/DNA molar ratio) were incubated with 200 ng of PvuII-restriction fragments (A) or supercoiled DNA (B) in binding buffer and then separated in an agarose gel. After staining with ethidium bromide, gels were photographed. Restriction DNA fragments containing the multi-cloning site and inserted ChIP sequences are marked by vertical arrows. Positions of supercoiled forms (monomer and dimer) of plasmid DNA after electrophoretic separation are marked by horizontal arrows. Slowly migrating complexes of p53 R273H protein and supercoiled DNA are marked by boxes.
Techniques Used: Recombinant, Chromatin Immunoprecipitation, In Vitro, Binding Assay, Plasmid Preparation, Incubation, Agarose Gel Electrophoresis, Staining, Clone Assay