restriction enzyme ecori hf  (New England Biolabs)


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  • 99
    Name:
    EcoRI HF
    Description:
    EcoRI HF 50 000 units
    Catalog Number:
    r3101l
    Price:
    249
    Size:
    50 000 units
    Category:
    Restriction Enzymes
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    Structured Review

    New England Biolabs restriction enzyme ecori hf
    EcoRI HF
    EcoRI HF 50 000 units
    https://www.bioz.com/result/restriction enzyme ecori hf/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    restriction enzyme ecori hf - by Bioz Stars, 2020-08
    99/100 stars

    Related Products / Commonly Used Together

    transconjugants plasmids

    Images

    1) Product Images from "Molecular Analysis of Antibiotic Resistance Determinants and Plasmids in Malaysian Isolates of Multidrug Resistant Klebsiella pneumoniae"

    Article Title: Molecular Analysis of Antibiotic Resistance Determinants and Plasmids in Malaysian Isolates of Multidrug Resistant Klebsiella pneumoniae

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0133654

    Dendrogram of EcoRI-digested plasmids from 27 transconjugants. 15 restriction profiles were identified (P1-P15). The dashed line represents the 80% similarity level used in cluster designation. Transconjugant plasmid ID, replicon and restriction profiles are shown.
    Figure Legend Snippet: Dendrogram of EcoRI-digested plasmids from 27 transconjugants. 15 restriction profiles were identified (P1-P15). The dashed line represents the 80% similarity level used in cluster designation. Transconjugant plasmid ID, replicon and restriction profiles are shown.

    Techniques Used: Plasmid Preparation

    Related Articles

    Agarose Gel Electrophoresis:

    Article Title: Efficient Dual-Negative Selection for Bacterial Genome Editing
    Article Snippet: .. Vectors were digested using EcoRI-HF and BamHI-HF (New England BioLabs) for 1h at 37°C, or PCR-amplified, and purified on agarose gel. ..

    Isolation:

    Article Title: Depurination of colibactin-derived interstrand cross-links
    Article Snippet: .. To set up the linearization reactions, 20 units of EcoRI-HF® (New England Biolabs®) was mixed with 500 ng of isolated DNA (40 units/µg DNA) in CutSmart® buffer (New England Biolabs®), pH 7.9, in a total volume of 50 µL for 30 min at 37 °C. .. The CutSmart® buffer (New England Biolabs®) contains 50 mM potassium acetate, 20 mM Tris-acetate, 10 mM magnesium acetate, and 100 µg/mL BSA.

    Purification:

    Article Title: Mobius Assembly: A versatile Golden-Gate framework towards universal DNA assembly
    Article Snippet: .. Subsequently the pSB1C3/pSB1K3 backbones and the Golden Gate cassettes were digested with EcoRI-HF and PstI-HF (NEB) for 20 min at 37°C followed by purification with PCR Clean-up kit (Macherey Nagel). .. Ligation was mediated by T7 DNA ligase (NEB) to construct mUAV, Level 1 and Level 2 Acceptor Vectors.

    Article Title: Depurination of colibactin-derived interstrand cross-links
    Article Snippet: .. To prepare the linearized DNA, the 2686 bp pUC19 vector (New England Biolabs®) was linearized with 20 units/µg EcoRI-HF® (New England Biolabs®) and the linearized DNA was purified using a PCR clean-up kit (New England Biolabs®), eluted into 10 mM Tris (pH 8.0), and quantified using a nanodrop. .. For each reaction with E.coli , 800 ng of linearized pUC19 DNA was added to 200 µL (6.2 µM base pairs) of M9-CA medium inoculated with 1.2 × 107 bacteria pre-grown to exponential phase in the M9-CA medium.

    Article Title: Efficient Dual-Negative Selection for Bacterial Genome Editing
    Article Snippet: .. Vectors were digested using EcoRI-HF and BamHI-HF (New England BioLabs) for 1h at 37°C, or PCR-amplified, and purified on agarose gel. ..

    Polymerase Chain Reaction:

    Article Title: Mobius Assembly: A versatile Golden-Gate framework towards universal DNA assembly
    Article Snippet: .. Subsequently the pSB1C3/pSB1K3 backbones and the Golden Gate cassettes were digested with EcoRI-HF and PstI-HF (NEB) for 20 min at 37°C followed by purification with PCR Clean-up kit (Macherey Nagel). .. Ligation was mediated by T7 DNA ligase (NEB) to construct mUAV, Level 1 and Level 2 Acceptor Vectors.

    Article Title: Depurination of colibactin-derived interstrand cross-links
    Article Snippet: .. To prepare the linearized DNA, the 2686 bp pUC19 vector (New England Biolabs®) was linearized with 20 units/µg EcoRI-HF® (New England Biolabs®) and the linearized DNA was purified using a PCR clean-up kit (New England Biolabs®), eluted into 10 mM Tris (pH 8.0), and quantified using a nanodrop. .. For each reaction with E.coli , 800 ng of linearized pUC19 DNA was added to 200 µL (6.2 µM base pairs) of M9-CA medium inoculated with 1.2 × 107 bacteria pre-grown to exponential phase in the M9-CA medium.

    Article Title: Efficient Dual-Negative Selection for Bacterial Genome Editing
    Article Snippet: .. Vectors were digested using EcoRI-HF and BamHI-HF (New England BioLabs) for 1h at 37°C, or PCR-amplified, and purified on agarose gel. ..

    Transformation Assay:

    Article Title: Genetic transformation of Spizellomyces punctatus, a resource for studying chytrid biology and evolutionary cell biology
    Article Snippet: .. 2.5 µg of genomic DNA of wild type (WT) and the four transformed strains of Spizellomyces (EM20C-1,2,3,4) was digested in a final volume of 50μL with 100U of EcoRI-HF (NEB R3101S) or HindIII-HF (NEB R3104S) for 24 hr at 37°C. .. After assessing the quality of the digestion by gel electrophoresis, the reaction was heat inactivated and 48µL of the digested DNA was incubated with 1µL of T4 ligase (400u/µL) for 48 hr at 4°C.

    Plasmid Preparation:

    Article Title: Depurination of colibactin-derived interstrand cross-links
    Article Snippet: .. To prepare the linearized DNA, the 2686 bp pUC19 vector (New England Biolabs®) was linearized with 20 units/µg EcoRI-HF® (New England Biolabs®) and the linearized DNA was purified using a PCR clean-up kit (New England Biolabs®), eluted into 10 mM Tris (pH 8.0), and quantified using a nanodrop. .. For each reaction with E.coli , 800 ng of linearized pUC19 DNA was added to 200 µL (6.2 µM base pairs) of M9-CA medium inoculated with 1.2 × 107 bacteria pre-grown to exponential phase in the M9-CA medium.

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  • 99
    New England Biolabs restriction enzyme ecori hf
    Dendrogram of <t>EcoRI-digested</t> plasmids from 27 <t>transconjugants.</t> 15 restriction profiles were identified (P1-P15). The dashed line represents the 80% similarity level used in cluster designation. Transconjugant plasmid ID, replicon and restriction profiles are shown.
    Restriction Enzyme Ecori Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction enzyme ecori hf/product/New England Biolabs
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    restriction enzyme ecori hf - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    New England Biolabs ecor1 hf restriction enzymes
    Dendrogram of <t>EcoRI-digested</t> plasmids from 27 <t>transconjugants.</t> 15 restriction profiles were identified (P1-P15). The dashed line represents the 80% similarity level used in cluster designation. Transconjugant plasmid ID, replicon and restriction profiles are shown.
    Ecor1 Hf Restriction Enzymes, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecor1 hf restriction enzymes/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ecor1 hf restriction enzymes - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    Image Search Results


    Dendrogram of EcoRI-digested plasmids from 27 transconjugants. 15 restriction profiles were identified (P1-P15). The dashed line represents the 80% similarity level used in cluster designation. Transconjugant plasmid ID, replicon and restriction profiles are shown.

    Journal: PLoS ONE

    Article Title: Molecular Analysis of Antibiotic Resistance Determinants and Plasmids in Malaysian Isolates of Multidrug Resistant Klebsiella pneumoniae

    doi: 10.1371/journal.pone.0133654

    Figure Lengend Snippet: Dendrogram of EcoRI-digested plasmids from 27 transconjugants. 15 restriction profiles were identified (P1-P15). The dashed line represents the 80% similarity level used in cluster designation. Transconjugant plasmid ID, replicon and restriction profiles are shown.

    Article Snippet: Transconjugants plasmids were digested with the restriction enzyme EcoRI -HF (New England Biolabs, UK) [ ].

    Techniques: Plasmid Preparation