restriction enzyme digest assay with btsci  (New England Biolabs)


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    New England Biolabs restriction enzyme digest assay with btsci
    (A) Working model showing emx2 induces prostaglandin biosynthesis for cilia development in zebrafish pronephros in both proximal and distal domain. (B) Image of DNA products after verifying <t>with</t> <t>BtsCI</t> restriction enzyme assay. − denotes samples without BtsCI and + denotes samples with BtsCI. WT embryos and confirmed WT embryos from emx2 el586/el586 line show two bands at 302 bp and 103 bp, while confirmed emx2 −/− embryos show one band at 405 bp. (C) WT embryos stained via WISH using marker for emx2 from 5 ss to 17 ss. Scale bar = 100 μm. (D) 28 hpf whole-mount IF for acetylated α-tubulin (cilia, green), γ-tubulin (basal bodies, red), and DAPI (nucleus, blue) in the distal pronephros of WT and emx2 −/− embryos. Scale bar = 50 μm. (E) Sanger sequencing between WT, WT from emx2 el586/el586 line and emx2 −/− embryos. (F) TALEN target site between WT and emx2 −/− . emx2 −/− has 10-bp deletion compared to WT. Green denotes L and R TALEN target sites. Blue denotes BtsCI restriction site which is disrupted in emx2 −/− embryos.
    Restriction Enzyme Digest Assay With Btsci, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction enzyme digest assay with btsci/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    restriction enzyme digest assay with btsci - by Bioz Stars, 2024-06
    95/100 stars

    Images

    1) Product Images from "Emx2 is an essential regulator of ciliated cell development across embryonic tissues"

    Article Title: Emx2 is an essential regulator of ciliated cell development across embryonic tissues

    Journal: bioRxiv

    doi: 10.1101/2024.01.04.574218

    (A) Working model showing emx2 induces prostaglandin biosynthesis for cilia development in zebrafish pronephros in both proximal and distal domain. (B) Image of DNA products after verifying with BtsCI restriction enzyme assay. − denotes samples without BtsCI and + denotes samples with BtsCI. WT embryos and confirmed WT embryos from emx2 el586/el586 line show two bands at 302 bp and 103 bp, while confirmed emx2 −/− embryos show one band at 405 bp. (C) WT embryos stained via WISH using marker for emx2 from 5 ss to 17 ss. Scale bar = 100 μm. (D) 28 hpf whole-mount IF for acetylated α-tubulin (cilia, green), γ-tubulin (basal bodies, red), and DAPI (nucleus, blue) in the distal pronephros of WT and emx2 −/− embryos. Scale bar = 50 μm. (E) Sanger sequencing between WT, WT from emx2 el586/el586 line and emx2 −/− embryos. (F) TALEN target site between WT and emx2 −/− . emx2 −/− has 10-bp deletion compared to WT. Green denotes L and R TALEN target sites. Blue denotes BtsCI restriction site which is disrupted in emx2 −/− embryos.
    Figure Legend Snippet: (A) Working model showing emx2 induces prostaglandin biosynthesis for cilia development in zebrafish pronephros in both proximal and distal domain. (B) Image of DNA products after verifying with BtsCI restriction enzyme assay. − denotes samples without BtsCI and + denotes samples with BtsCI. WT embryos and confirmed WT embryos from emx2 el586/el586 line show two bands at 302 bp and 103 bp, while confirmed emx2 −/− embryos show one band at 405 bp. (C) WT embryos stained via WISH using marker for emx2 from 5 ss to 17 ss. Scale bar = 100 μm. (D) 28 hpf whole-mount IF for acetylated α-tubulin (cilia, green), γ-tubulin (basal bodies, red), and DAPI (nucleus, blue) in the distal pronephros of WT and emx2 −/− embryos. Scale bar = 50 μm. (E) Sanger sequencing between WT, WT from emx2 el586/el586 line and emx2 −/− embryos. (F) TALEN target site between WT and emx2 −/− . emx2 −/− has 10-bp deletion compared to WT. Green denotes L and R TALEN target sites. Blue denotes BtsCI restriction site which is disrupted in emx2 −/− embryos.

    Techniques Used: Enzymatic Assay, Staining, Marker, Sequencing

    restriction enzyme digest assay with btsci  (New England Biolabs)


    Bioz Verified Symbol New England Biolabs is a verified supplier
    Bioz Manufacturer Symbol New England Biolabs manufactures this product  
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  • 95

    Structured Review

    New England Biolabs restriction enzyme digest assay with btsci
    (A) Working model showing emx2 induces prostaglandin biosynthesis for cilia development in zebrafish pronephros in both proximal and distal domain. (B) Image of DNA products after verifying <t>with</t> <t>BtsCI</t> restriction enzyme assay. − denotes samples without BtsCI and + denotes samples with BtsCI. WT embryos and confirmed WT embryos from emx2 el586/el586 line show two bands at 302 bp and 103 bp, while confirmed emx2 −/− embryos show one band at 405 bp. (C) WT embryos stained via WISH using marker for emx2 from 5 ss to 17 ss. Scale bar = 100 μm. (D) 28 hpf whole-mount IF for acetylated α-tubulin (cilia, green), γ-tubulin (basal bodies, red), and DAPI (nucleus, blue) in the distal pronephros of WT and emx2 −/− embryos. Scale bar = 50 μm. (E) Sanger sequencing between WT, WT from emx2 el586/el586 line and emx2 −/− embryos. (F) TALEN target site between WT and emx2 −/− . emx2 −/− has 10-bp deletion compared to WT. Green denotes L and R TALEN target sites. Blue denotes BtsCI restriction site which is disrupted in emx2 −/− embryos.
    Restriction Enzyme Digest Assay With Btsci, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction enzyme digest assay with btsci/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    restriction enzyme digest assay with btsci - by Bioz Stars, 2024-06
    95/100 stars

    Images

    1) Product Images from "Emx2 is an essential regulator of ciliated cell development across embryonic tissues"

    Article Title: Emx2 is an essential regulator of ciliated cell development across embryonic tissues

    Journal: bioRxiv

    doi: 10.1101/2024.01.04.574218

    (A) Working model showing emx2 induces prostaglandin biosynthesis for cilia development in zebrafish pronephros in both proximal and distal domain. (B) Image of DNA products after verifying with BtsCI restriction enzyme assay. − denotes samples without BtsCI and + denotes samples with BtsCI. WT embryos and confirmed WT embryos from emx2 el586/el586 line show two bands at 302 bp and 103 bp, while confirmed emx2 −/− embryos show one band at 405 bp. (C) WT embryos stained via WISH using marker for emx2 from 5 ss to 17 ss. Scale bar = 100 μm. (D) 28 hpf whole-mount IF for acetylated α-tubulin (cilia, green), γ-tubulin (basal bodies, red), and DAPI (nucleus, blue) in the distal pronephros of WT and emx2 −/− embryos. Scale bar = 50 μm. (E) Sanger sequencing between WT, WT from emx2 el586/el586 line and emx2 −/− embryos. (F) TALEN target site between WT and emx2 −/− . emx2 −/− has 10-bp deletion compared to WT. Green denotes L and R TALEN target sites. Blue denotes BtsCI restriction site which is disrupted in emx2 −/− embryos.
    Figure Legend Snippet: (A) Working model showing emx2 induces prostaglandin biosynthesis for cilia development in zebrafish pronephros in both proximal and distal domain. (B) Image of DNA products after verifying with BtsCI restriction enzyme assay. − denotes samples without BtsCI and + denotes samples with BtsCI. WT embryos and confirmed WT embryos from emx2 el586/el586 line show two bands at 302 bp and 103 bp, while confirmed emx2 −/− embryos show one band at 405 bp. (C) WT embryos stained via WISH using marker for emx2 from 5 ss to 17 ss. Scale bar = 100 μm. (D) 28 hpf whole-mount IF for acetylated α-tubulin (cilia, green), γ-tubulin (basal bodies, red), and DAPI (nucleus, blue) in the distal pronephros of WT and emx2 −/− embryos. Scale bar = 50 μm. (E) Sanger sequencing between WT, WT from emx2 el586/el586 line and emx2 −/− embryos. (F) TALEN target site between WT and emx2 −/− . emx2 −/− has 10-bp deletion compared to WT. Green denotes L and R TALEN target sites. Blue denotes BtsCI restriction site which is disrupted in emx2 −/− embryos.

    Techniques Used: Enzymatic Assay, Staining, Marker, Sequencing

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    New England Biolabs restriction enzyme digest assay with btsci
    (A) Working model showing emx2 induces prostaglandin biosynthesis for cilia development in zebrafish pronephros in both proximal and distal domain. (B) Image of DNA products after verifying <t>with</t> <t>BtsCI</t> restriction enzyme assay. − denotes samples without BtsCI and + denotes samples with BtsCI. WT embryos and confirmed WT embryos from emx2 el586/el586 line show two bands at 302 bp and 103 bp, while confirmed emx2 −/− embryos show one band at 405 bp. (C) WT embryos stained via WISH using marker for emx2 from 5 ss to 17 ss. Scale bar = 100 μm. (D) 28 hpf whole-mount IF for acetylated α-tubulin (cilia, green), γ-tubulin (basal bodies, red), and DAPI (nucleus, blue) in the distal pronephros of WT and emx2 −/− embryos. Scale bar = 50 μm. (E) Sanger sequencing between WT, WT from emx2 el586/el586 line and emx2 −/− embryos. (F) TALEN target site between WT and emx2 −/− . emx2 −/− has 10-bp deletion compared to WT. Green denotes L and R TALEN target sites. Blue denotes BtsCI restriction site which is disrupted in emx2 −/− embryos.
    Restriction Enzyme Digest Assay With Btsci, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction enzyme digest assay with btsci/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    restriction enzyme digest assay with btsci - by Bioz Stars, 2024-06
    95/100 stars
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    (A) Working model showing emx2 induces prostaglandin biosynthesis for cilia development in zebrafish pronephros in both proximal and distal domain. (B) Image of DNA products after verifying with BtsCI restriction enzyme assay. − denotes samples without BtsCI and + denotes samples with BtsCI. WT embryos and confirmed WT embryos from emx2 el586/el586 line show two bands at 302 bp and 103 bp, while confirmed emx2 −/− embryos show one band at 405 bp. (C) WT embryos stained via WISH using marker for emx2 from 5 ss to 17 ss. Scale bar = 100 μm. (D) 28 hpf whole-mount IF for acetylated α-tubulin (cilia, green), γ-tubulin (basal bodies, red), and DAPI (nucleus, blue) in the distal pronephros of WT and emx2 −/− embryos. Scale bar = 50 μm. (E) Sanger sequencing between WT, WT from emx2 el586/el586 line and emx2 −/− embryos. (F) TALEN target site between WT and emx2 −/− . emx2 −/− has 10-bp deletion compared to WT. Green denotes L and R TALEN target sites. Blue denotes BtsCI restriction site which is disrupted in emx2 −/− embryos.

    Journal: bioRxiv

    Article Title: Emx2 is an essential regulator of ciliated cell development across embryonic tissues

    doi: 10.1101/2024.01.04.574218

    Figure Lengend Snippet: (A) Working model showing emx2 induces prostaglandin biosynthesis for cilia development in zebrafish pronephros in both proximal and distal domain. (B) Image of DNA products after verifying with BtsCI restriction enzyme assay. − denotes samples without BtsCI and + denotes samples with BtsCI. WT embryos and confirmed WT embryos from emx2 el586/el586 line show two bands at 302 bp and 103 bp, while confirmed emx2 −/− embryos show one band at 405 bp. (C) WT embryos stained via WISH using marker for emx2 from 5 ss to 17 ss. Scale bar = 100 μm. (D) 28 hpf whole-mount IF for acetylated α-tubulin (cilia, green), γ-tubulin (basal bodies, red), and DAPI (nucleus, blue) in the distal pronephros of WT and emx2 −/− embryos. Scale bar = 50 μm. (E) Sanger sequencing between WT, WT from emx2 el586/el586 line and emx2 −/− embryos. (F) TALEN target site between WT and emx2 −/− . emx2 −/− has 10-bp deletion compared to WT. Green denotes L and R TALEN target sites. Blue denotes BtsCI restriction site which is disrupted in emx2 −/− embryos.

    Article Snippet: Additionally, restriction enzyme digest assay with BtsCI (New England Biolabs) was performed to identify WT, heterozygous or mutant samples.

    Techniques: Enzymatic Assay, Staining, Marker, Sequencing