restriction enzyme blpi  (New England Biolabs)


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    New England Biolabs restriction enzyme blpi
    Molecular analysis of mtDNA replication in Polg A449T/A449T mitochondria. ( A ) Southern blot analysis of <t>BlpI-digested</t> mtDNA and 7S <t>DNA</t> from SKM and liver of WT and Polg A449T/A449T animals. ( B ) Quantification of the Southern blots presented in panel (A) and Supplementary Figure S6A–C . 7S DNA levels were normalized to linearized full length mtDNA and presented as FOLD change from WT animals. Data are presented as mean ± SEM. Two tailed unpaired Student's t -test: * P
    Restriction Enzyme Blpi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction enzyme blpi/product/New England Biolabs
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    restriction enzyme blpi - by Bioz Stars, 2022-08
    93/100 stars

    Images

    1) Product Images from "DNA polymerase gamma mutations that impair holoenzyme stability cause catalytic subunit depletion"

    Article Title: DNA polymerase gamma mutations that impair holoenzyme stability cause catalytic subunit depletion

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkab282

    Molecular analysis of mtDNA replication in Polg A449T/A449T mitochondria. ( A ) Southern blot analysis of BlpI-digested mtDNA and 7S DNA from SKM and liver of WT and Polg A449T/A449T animals. ( B ) Quantification of the Southern blots presented in panel (A) and Supplementary Figure S6A–C . 7S DNA levels were normalized to linearized full length mtDNA and presented as FOLD change from WT animals. Data are presented as mean ± SEM. Two tailed unpaired Student's t -test: * P
    Figure Legend Snippet: Molecular analysis of mtDNA replication in Polg A449T/A449T mitochondria. ( A ) Southern blot analysis of BlpI-digested mtDNA and 7S DNA from SKM and liver of WT and Polg A449T/A449T animals. ( B ) Quantification of the Southern blots presented in panel (A) and Supplementary Figure S6A–C . 7S DNA levels were normalized to linearized full length mtDNA and presented as FOLD change from WT animals. Data are presented as mean ± SEM. Two tailed unpaired Student's t -test: * P

    Techniques Used: Southern Blot, Two Tailed Test

    2) Product Images from "In vivo and in vitro mechanistic characterization of a clinically relevant PolγA mutation"

    Article Title: In vivo and in vitro mechanistic characterization of a clinically relevant PolγA mutation

    Journal: bioRxiv

    doi: 10.1101/2020.09.10.291369

    Molecular analysis of mtDNA replication in Polg A449T/A449T mitochondria. A. Southern blot analysis of BlpI-digested mtDNA and 7S DNA from skeletal muscle (SKM) and liver of WT and Polg A449T/A449T animals. B. Quantification of the Southern blots presented in panel A and Supplemental Figure 4A-C . 7S DNA levels were normalized to linearized full length mtDNA and presented as FOLD change from WT animals. Data are presented as mean ± SEM. *p
    Figure Legend Snippet: Molecular analysis of mtDNA replication in Polg A449T/A449T mitochondria. A. Southern blot analysis of BlpI-digested mtDNA and 7S DNA from skeletal muscle (SKM) and liver of WT and Polg A449T/A449T animals. B. Quantification of the Southern blots presented in panel A and Supplemental Figure 4A-C . 7S DNA levels were normalized to linearized full length mtDNA and presented as FOLD change from WT animals. Data are presented as mean ± SEM. *p

    Techniques Used: Southern Blot

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    New England Biolabs restriction enzyme blpi
    Molecular analysis of mtDNA replication in Polg A449T/A449T mitochondria. ( A ) Southern blot analysis of <t>BlpI-digested</t> mtDNA and 7S <t>DNA</t> from SKM and liver of WT and Polg A449T/A449T animals. ( B ) Quantification of the Southern blots presented in panel (A) and Supplementary Figure S6A–C . 7S DNA levels were normalized to linearized full length mtDNA and presented as FOLD change from WT animals. Data are presented as mean ± SEM. Two tailed unpaired Student's t -test: * P
    Restriction Enzyme Blpi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction enzyme blpi/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    restriction enzyme blpi - by Bioz Stars, 2022-08
    93/100 stars
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    Molecular analysis of mtDNA replication in Polg A449T/A449T mitochondria. ( A ) Southern blot analysis of BlpI-digested mtDNA and 7S DNA from SKM and liver of WT and Polg A449T/A449T animals. ( B ) Quantification of the Southern blots presented in panel (A) and Supplementary Figure S6A–C . 7S DNA levels were normalized to linearized full length mtDNA and presented as FOLD change from WT animals. Data are presented as mean ± SEM. Two tailed unpaired Student's t -test: * P

    Journal: Nucleic Acids Research

    Article Title: DNA polymerase gamma mutations that impair holoenzyme stability cause catalytic subunit depletion

    doi: 10.1093/nar/gkab282

    Figure Lengend Snippet: Molecular analysis of mtDNA replication in Polg A449T/A449T mitochondria. ( A ) Southern blot analysis of BlpI-digested mtDNA and 7S DNA from SKM and liver of WT and Polg A449T/A449T animals. ( B ) Quantification of the Southern blots presented in panel (A) and Supplementary Figure S6A–C . 7S DNA levels were normalized to linearized full length mtDNA and presented as FOLD change from WT animals. Data are presented as mean ± SEM. Two tailed unpaired Student's t -test: * P

    Article Snippet: Southern blot Three micrograms of total DNA isolated from each tissue were restricted using the restriction enzyme BlpI according to manufacturer's instructions (New England Biolabs).

    Techniques: Southern Blot, Two Tailed Test

    Molecular analysis of mtDNA replication in Polg A449T/A449T mitochondria. A. Southern blot analysis of BlpI-digested mtDNA and 7S DNA from skeletal muscle (SKM) and liver of WT and Polg A449T/A449T animals. B. Quantification of the Southern blots presented in panel A and Supplemental Figure 4A-C . 7S DNA levels were normalized to linearized full length mtDNA and presented as FOLD change from WT animals. Data are presented as mean ± SEM. *p

    Journal: bioRxiv

    Article Title: In vivo and in vitro mechanistic characterization of a clinically relevant PolγA mutation

    doi: 10.1101/2020.09.10.291369

    Figure Lengend Snippet: Molecular analysis of mtDNA replication in Polg A449T/A449T mitochondria. A. Southern blot analysis of BlpI-digested mtDNA and 7S DNA from skeletal muscle (SKM) and liver of WT and Polg A449T/A449T animals. B. Quantification of the Southern blots presented in panel A and Supplemental Figure 4A-C . 7S DNA levels were normalized to linearized full length mtDNA and presented as FOLD change from WT animals. Data are presented as mean ± SEM. *p

    Article Snippet: Southern Blot Three micrograms of total DNA isolated from each tissue were restricted using the restriction enzyme BlpI according to manufacturer’s instructions (New England Biolabs).

    Techniques: Southern Blot