restriction endonucleases mse i  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 77

    Structured Review

    New England Biolabs restriction endonucleases mse i
    Restriction profiles obtained after digestion of polymerase chain reaction products from the ITS1 locus with <t>Mse</t> I. lanes 1- 3-, 100-, 50-, and 10-bp ladder molecular weight markers; lanes 4 and 15, bovine genotype 2 isolates; lanes 5, 6, 14, and 16, human genotype 1 isolates including DE340 (lane 14); lanes 7 and 9–12, cervine genotype isolates including MH205 (lane 7), TK320 (lane 10), and DE302 (lane 11); lane 8, Cryptosporidium meleagridis isolate CS33; lanes 13 and 17, other novel genotype isolates such as VF383 (lane 13) and TK348 (lane 17)
    Restriction Endonucleases Mse I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 77/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction endonucleases mse i/product/New England Biolabs
    Average 77 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    restriction endonucleases mse i - by Bioz Stars, 2020-03
    77/100 stars

    Images

    1) Product Images from "Novel Cryptosporidium Genotypes in Sporadic Cryptosporidiosis Cases: First Report of Human Infections with a Cervine Genotype"

    Article Title: Novel Cryptosporidium Genotypes in Sporadic Cryptosporidiosis Cases: First Report of Human Infections with a Cervine Genotype

    Journal: Emerging Infectious Diseases

    doi: 10.3201/eid0803.010194

    Restriction profiles obtained after digestion of polymerase chain reaction products from the ITS1 locus with Mse I. lanes 1- 3-, 100-, 50-, and 10-bp ladder molecular weight markers; lanes 4 and 15, bovine genotype 2 isolates; lanes 5, 6, 14, and 16, human genotype 1 isolates including DE340 (lane 14); lanes 7 and 9–12, cervine genotype isolates including MH205 (lane 7), TK320 (lane 10), and DE302 (lane 11); lane 8, Cryptosporidium meleagridis isolate CS33; lanes 13 and 17, other novel genotype isolates such as VF383 (lane 13) and TK348 (lane 17)
    Figure Legend Snippet: Restriction profiles obtained after digestion of polymerase chain reaction products from the ITS1 locus with Mse I. lanes 1- 3-, 100-, 50-, and 10-bp ladder molecular weight markers; lanes 4 and 15, bovine genotype 2 isolates; lanes 5, 6, 14, and 16, human genotype 1 isolates including DE340 (lane 14); lanes 7 and 9–12, cervine genotype isolates including MH205 (lane 7), TK320 (lane 10), and DE302 (lane 11); lane 8, Cryptosporidium meleagridis isolate CS33; lanes 13 and 17, other novel genotype isolates such as VF383 (lane 13) and TK348 (lane 17)

    Techniques Used: Polymerase Chain Reaction, Molecular Weight

    Related Articles

    Amplification:

    Article Title: Development and characterization of 11 polymorphic microsatellite markers in Tapiscia sinensis (Staphyleaceae) 1
    Article Snippet: A total of 250 ng of genomic DNA was digested with the restriction endonuclease Mse I (New England Biolabs, Beverly, Massachusetts, USA) and ligated with Mse I AFLP adapter (5′-TACTCAGGACTCAT-3′/5′-GACGATGAGTCCTGAG 3′) using the T4 DNA ligase (TaKaRa Biotechnology Co., Dalian, China). .. The digestion-ligation mixture was diluted (1:10) and PCR amplified using AFLP adapter-specific primers (5′-GATGAGTCCTGAGTAAN-3′, i.e., Mse I-N) for 18 cycles in a total volume of 20 μL.

    Mutagenesis:

    Article Title: Multiplex Real-Time PCR Assays that Measure the Abundance of Extremely Rare Mutations Associated with Cancer
    Article Snippet: .. The mutant and wild-type EGFR plasmids, and the human genomic DNAs, were digested by incubation with restriction endonuclease Mse I (New England Biolabs, Ipswich, MA). ..

    Isolation:

    Article Title: Multiplex Real-Time PCR Assays that Measure the Abundance of Extremely Rare Mutations Associated with Cancer
    Article Snippet: Human genomic DNA encoding the EGFR L858R mutation was isolated from cell line H1975 (CRL-5908, American Type Culture Collection, Manassas, VA) and human genomic DNA containing the corresponding wild-type sequence was purchased from Coriell Cell Repositories (Camden, NJ). .. The mutant and wild-type EGFR plasmids, and the human genomic DNAs, were digested by incubation with restriction endonuclease Mse I (New England Biolabs, Ipswich, MA).

    Article Title: Development and characterization of 11 polymorphic microsatellite markers in Tapiscia sinensis (Staphyleaceae) 1
    Article Snippet: An enriched partial genomic library was constructed using a Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) protocol described by , with some modifications. .. A total of 250 ng of genomic DNA was digested with the restriction endonuclease Mse I (New England Biolabs, Beverly, Massachusetts, USA) and ligated with Mse I AFLP adapter (5′-TACTCAGGACTCAT-3′/5′-GACGATGAGTCCTGAG 3′) using the T4 DNA ligase (TaKaRa Biotechnology Co., Dalian, China).

    Construct:

    Article Title: Development and characterization of 11 polymorphic microsatellite markers in Tapiscia sinensis (Staphyleaceae) 1
    Article Snippet: An enriched partial genomic library was constructed using a Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) protocol described by , with some modifications. .. A total of 250 ng of genomic DNA was digested with the restriction endonuclease Mse I (New England Biolabs, Beverly, Massachusetts, USA) and ligated with Mse I AFLP adapter (5′-TACTCAGGACTCAT-3′/5′-GACGATGAGTCCTGAG 3′) using the T4 DNA ligase (TaKaRa Biotechnology Co., Dalian, China).

    Purification:

    Article Title: Novel Cryptosporidium Genotypes in Sporadic Cryptosporidiosis Cases: First Report of Human Infections with a Cervine Genotype
    Article Snippet: .. RFLP Analyses of PCR Products PCR products were purified by using QIAquick spin columns (Qiagen, Mississauga, ON) according to the manufacturer’s instructions before digestion with the restriction endonucleases Mse I (New England BioLabs, Mississauga, ON) for the ITS1 locus and Rsa I (New England BioLabs) for the COWP gene. ..

    Sequencing:

    Article Title: Multiplex Real-Time PCR Assays that Measure the Abundance of Extremely Rare Mutations Associated with Cancer
    Article Snippet: Human genomic DNA encoding the EGFR L858R mutation was isolated from cell line H1975 (CRL-5908, American Type Culture Collection, Manassas, VA) and human genomic DNA containing the corresponding wild-type sequence was purchased from Coriell Cell Repositories (Camden, NJ). .. The mutant and wild-type EGFR plasmids, and the human genomic DNAs, were digested by incubation with restriction endonuclease Mse I (New England Biolabs, Ipswich, MA).

    Incubation:

    Article Title: Multiplex Real-Time PCR Assays that Measure the Abundance of Extremely Rare Mutations Associated with Cancer
    Article Snippet: .. The mutant and wild-type EGFR plasmids, and the human genomic DNAs, were digested by incubation with restriction endonuclease Mse I (New England Biolabs, Ipswich, MA). ..

    Article Title: Development and characterization of 11 polymorphic microsatellite markers in Tapiscia sinensis (Staphyleaceae) 1
    Article Snippet: A total of 250 ng of genomic DNA was digested with the restriction endonuclease Mse I (New England Biolabs, Beverly, Massachusetts, USA) and ligated with Mse I AFLP adapter (5′-TACTCAGGACTCAT-3′/5′-GACGATGAGTCCTGAG 3′) using the T4 DNA ligase (TaKaRa Biotechnology Co., Dalian, China). .. The DNA was denatured for 5 min at 95°C and incubated at 48°C for 2 h. The hybridization products were selectively captured with 300 μL streptavidin-coated beads (Promega Corporation, Madison, Wisconsin, USA).

    Polymerase Chain Reaction:

    Article Title: Novel Cryptosporidium Genotypes in Sporadic Cryptosporidiosis Cases: First Report of Human Infections with a Cervine Genotype
    Article Snippet: .. RFLP Analyses of PCR Products PCR products were purified by using QIAquick spin columns (Qiagen, Mississauga, ON) according to the manufacturer’s instructions before digestion with the restriction endonucleases Mse I (New England BioLabs, Mississauga, ON) for the ITS1 locus and Rsa I (New England BioLabs) for the COWP gene. ..

    Article Title: Development and characterization of 11 polymorphic microsatellite markers in Tapiscia sinensis (Staphyleaceae) 1
    Article Snippet: A total of 250 ng of genomic DNA was digested with the restriction endonuclease Mse I (New England Biolabs, Beverly, Massachusetts, USA) and ligated with Mse I AFLP adapter (5′-TACTCAGGACTCAT-3′/5′-GACGATGAGTCCTGAG 3′) using the T4 DNA ligase (TaKaRa Biotechnology Co., Dalian, China). .. The digestion-ligation mixture was diluted (1:10) and PCR amplified using AFLP adapter-specific primers (5′-GATGAGTCCTGAGTAAN-3′, i.e., Mse I-N) for 18 cycles in a total volume of 20 μL.

    Staining:

    Article Title: Novel Cryptosporidium Genotypes in Sporadic Cryptosporidiosis Cases: First Report of Human Infections with a Cervine Genotype
    Article Snippet: RFLP Analyses of PCR Products PCR products were purified by using QIAquick spin columns (Qiagen, Mississauga, ON) according to the manufacturer’s instructions before digestion with the restriction endonucleases Mse I (New England BioLabs, Mississauga, ON) for the ITS1 locus and Rsa I (New England BioLabs) for the COWP gene. .. Restriction fragments were then separated on Metaphor FMC agarose gels (3% for Mse I digests of ITS1 products and 3.2% for Rsa I digests of COWP products) (Mandel Scientific, Guelph, ON) and stained with ethidium bromide; the patterns were visualized with a UV transilluminator.

    Plasmid Preparation:

    Article Title: Multiplex Real-Time PCR Assays that Measure the Abundance of Extremely Rare Mutations Associated with Cancer
    Article Snippet: DNA templates Plasmids containing EGFR sequences (either the L858 mutant sequence or the wild-type sequence) were prepared by inserting a 115-base pair gene fragment into a pGEM® -11Zf(+) vector (Promega, Madison, WI). .. The mutant and wild-type EGFR plasmids, and the human genomic DNAs, were digested by incubation with restriction endonuclease Mse I (New England Biolabs, Ipswich, MA).

    Hybridization:

    Article Title: Development and characterization of 11 polymorphic microsatellite markers in Tapiscia sinensis (Staphyleaceae) 1
    Article Snippet: A total of 250 ng of genomic DNA was digested with the restriction endonuclease Mse I (New England Biolabs, Beverly, Massachusetts, USA) and ligated with Mse I AFLP adapter (5′-TACTCAGGACTCAT-3′/5′-GACGATGAGTCCTGAG 3′) using the T4 DNA ligase (TaKaRa Biotechnology Co., Dalian, China). .. The thermocycler conditions were as follows: 30 s denaturation at 94°C, annealing at 53°C for 60 s, and a final extension at 72°C for 60 s. After denaturation at 95°C for 5 min, 500 ng of amplified DNA fragments with a size range of 500–750 bp were hybridized with 150 pmol of 5′-biotinylated (AC)15 probes in 250 μL of hybridization buffer containing saline sodium citrate (SSC) 4.7× and sodium dodecyl sulfate (SDS) 0.07%.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 77
    New England Biolabs restriction endonucleases mse i
    Restriction profiles obtained after digestion of polymerase chain reaction products from the ITS1 locus with <t>Mse</t> I. lanes 1- 3-, 100-, 50-, and 10-bp ladder molecular weight markers; lanes 4 and 15, bovine genotype 2 isolates; lanes 5, 6, 14, and 16, human genotype 1 isolates including DE340 (lane 14); lanes 7 and 9–12, cervine genotype isolates including MH205 (lane 7), TK320 (lane 10), and DE302 (lane 11); lane 8, Cryptosporidium meleagridis isolate CS33; lanes 13 and 17, other novel genotype isolates such as VF383 (lane 13) and TK348 (lane 17)
    Restriction Endonucleases Mse I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 77/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction endonucleases mse i/product/New England Biolabs
    Average 77 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    restriction endonucleases mse i - by Bioz Stars, 2020-03
    77/100 stars
      Buy from Supplier

    Image Search Results


    Restriction profiles obtained after digestion of polymerase chain reaction products from the ITS1 locus with Mse I. lanes 1- 3-, 100-, 50-, and 10-bp ladder molecular weight markers; lanes 4 and 15, bovine genotype 2 isolates; lanes 5, 6, 14, and 16, human genotype 1 isolates including DE340 (lane 14); lanes 7 and 9–12, cervine genotype isolates including MH205 (lane 7), TK320 (lane 10), and DE302 (lane 11); lane 8, Cryptosporidium meleagridis isolate CS33; lanes 13 and 17, other novel genotype isolates such as VF383 (lane 13) and TK348 (lane 17)

    Journal: Emerging Infectious Diseases

    Article Title: Novel Cryptosporidium Genotypes in Sporadic Cryptosporidiosis Cases: First Report of Human Infections with a Cervine Genotype

    doi: 10.3201/eid0803.010194

    Figure Lengend Snippet: Restriction profiles obtained after digestion of polymerase chain reaction products from the ITS1 locus with Mse I. lanes 1- 3-, 100-, 50-, and 10-bp ladder molecular weight markers; lanes 4 and 15, bovine genotype 2 isolates; lanes 5, 6, 14, and 16, human genotype 1 isolates including DE340 (lane 14); lanes 7 and 9–12, cervine genotype isolates including MH205 (lane 7), TK320 (lane 10), and DE302 (lane 11); lane 8, Cryptosporidium meleagridis isolate CS33; lanes 13 and 17, other novel genotype isolates such as VF383 (lane 13) and TK348 (lane 17)

    Article Snippet: RFLP Analyses of PCR Products PCR products were purified by using QIAquick spin columns (Qiagen, Mississauga, ON) according to the manufacturer’s instructions before digestion with the restriction endonucleases Mse I (New England BioLabs, Mississauga, ON) for the ITS1 locus and Rsa I (New England BioLabs) for the COWP gene.

    Techniques: Polymerase Chain Reaction, Molecular Weight

    Isolation of single fluorescent cells from bone marrow by micromanipulation and preparation of DNA after proteinase K (PK) treatment. After PK inactivation, the double-stranded DNA was digested with Mse I, leaving a TA overhang for adapter annealing and subsequent ligation. After primary amplification, 1/100 of the PCR products was reamplified in the presence of bio-UTP, and 2 μg were used for hybridization.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Comparative genomic hybridization, loss of heterozygosity, and DNA sequence analysis of single cells

    doi:

    Figure Lengend Snippet: Isolation of single fluorescent cells from bone marrow by micromanipulation and preparation of DNA after proteinase K (PK) treatment. After PK inactivation, the double-stranded DNA was digested with Mse I, leaving a TA overhang for adapter annealing and subsequent ligation. After primary amplification, 1/100 of the PCR products was reamplified in the presence of bio-UTP, and 2 μg were used for hybridization.

    Article Snippet: Proteinase K was inactivated at 80°C for 10 min. After inactivation of proteinase K, Mse I restriction endonuclease digest was performed in 5 μl by adding 0.2 μl of 10× One-Phor-All-Buffer-Plus, 0.5 μl of Mse I (10 units; New England Biolabs), and 1.3 μl of H2 O for 3 h at 37°C.

    Techniques: Isolation, Micromanipulation, Ligation, Amplification, Polymerase Chain Reaction, Hybridization

    Targeted sequencing library preparation method . (a) Overview of the assay. (b) Specific preparation steps: (1) genomic DNA is digested using Mse I restriction endonuclease. (2) Then, genomic DNA fragments are circularized using thermostable DNA ligase and Taq DNA polymerase for 5' editing. Pool of oligonucleotides targeting 5' and 3' ends of the DNA fragments and vector oligonucleotide are used for targeted DNA capture. (3) After circularization, regular Illumina sequencing library can be prepared by PCR. (4) PCR amplified library fragments are similar to regular Illumina library constructs and anneal to immobilized primers on the flow cell. (5) Additionally, circular constructs can be directly sequenced as the adapted genomic DNA circles incorporate all DNA components required for library immobilization and sequencing. (c) Molecular structures of vector oligonucleotide and capture oligonucleotides.

    Journal: BMC Biotechnology

    Article Title: Targeted sequencing library preparation by genomic DNA circularization

    doi: 10.1186/1472-6750-11-122

    Figure Lengend Snippet: Targeted sequencing library preparation method . (a) Overview of the assay. (b) Specific preparation steps: (1) genomic DNA is digested using Mse I restriction endonuclease. (2) Then, genomic DNA fragments are circularized using thermostable DNA ligase and Taq DNA polymerase for 5' editing. Pool of oligonucleotides targeting 5' and 3' ends of the DNA fragments and vector oligonucleotide are used for targeted DNA capture. (3) After circularization, regular Illumina sequencing library can be prepared by PCR. (4) PCR amplified library fragments are similar to regular Illumina library constructs and anneal to immobilized primers on the flow cell. (5) Additionally, circular constructs can be directly sequenced as the adapted genomic DNA circles incorporate all DNA components required for library immobilization and sequencing. (c) Molecular structures of vector oligonucleotide and capture oligonucleotides.

    Article Snippet: We digested 1 μg of genomic DNA from NA18507 with Mse I restriction endonuclease (NEB) for 3 hours in 37°C, followed by a heat inactivation of the enzyme for 20 min in 65°C.

    Techniques: Sequencing, Plasmid Preparation, Polymerase Chain Reaction, Amplification, Construct, Flow Cytometry