restriction endonucleases ecori  (Thermo Fisher)


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    Name:
    EcoRI 10 U µL
    Description:
    5 G ↓A A T T C 3 3 C T T A A ↑G 5 Thermo Scientific EcoRI restriction enzyme recognizes G AATTC sites and cuts best at 37°C in its own unique buffer See Reaction Conditions for Restriction Enzymes for a table of enzyme activity conditions for double digestion and heat inactivation for this and other restriction enzymes Note Also available as a FastDigest enzyme for rapid DNA digestion Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes optimized to work in one of the buffers of the Five Buffer System In addition the universal Tango buffer is provided for convenience in double digestions All of the enzymes exhibit 100 activity in the recommended buffer and reaction conditions To ensure consistent performance Thermo Scientific restriction enzyme reaction buffers contain premixed BSA which enhances the stability of many enzymes and binds contaminants that sent in DNA preparations Features• Superior quality stringent quality control and industry leading manufacturing process• Convenient color coded Five Buffer System• Includes universal Tango buffer for double digestions• BSA premixed in reaction buffers• Wide selection of restriction endonuclease specificitiesApplications• Molecular cloning• Restriction site mapping• Genotyping• Southern blotting• Restriction fragment length polymorphism RFLP • SNPNote For methylation sensitivity refer to product specifications
    Catalog Number:
    er0271
    Price:
    None
    Applications:
    Cloning|Restriction Enzyme Cloning
    Category:
    Proteins Enzymes Peptides
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    Structured Review

    Thermo Fisher restriction endonucleases ecori
    The main features of pSJ2 and pSJ3, plasmids useful for measuring <t>DNA</t> polymerase fidelity. (A) The lacZα peptide and lac promoter sequences of M13mp2, pSJ1, pSJ2, and pSJ3. Only the bases actually used in fidelity determination are shown; thus, pSJ1 and pSJ3 contain the lac promoter, but because of the nicking site positions, this element is not used in fidelity assays with these two plasmids. The symbol ∗ indicates identical bases in the lacZα peptide (for all four sequences) and in the lac promoter (for M13mp2 and pSJ2). The underlined sequences are the extra bases at the extremities of pSJ2, necessary for introducing the nicking sites. The ATG triplet that encodes the first methionine in the lacZα peptide is shown, as is the dam methylase (GATC) site altered in pSJ2 and pSJ3. The C shown with the symbol ↓ is the base altered in pSJ2 to introduce a stop codon for expression frequency determination. In all cases, the coding sequence of lacZα is given, which corresponds to the bases on the inner circle of the plasmids illustrated. (B) Plasmid maps of pSJ2 and pSJ3 showing the location and orientation of the lacZα sequences and the nicking endonuclease sites, N(t/b)BbvCI for pSJ2 and N(t/b)Bpu10I for pSJ3. The <t>EcoRI</t> site used for analytical purposes is also shown. The abbreviations dcs and ucs stand for downstream and upstream cutting sites, respectively.
    5 G ↓A A T T C 3 3 C T T A A ↑G 5 Thermo Scientific EcoRI restriction enzyme recognizes G AATTC sites and cuts best at 37°C in its own unique buffer See Reaction Conditions for Restriction Enzymes for a table of enzyme activity conditions for double digestion and heat inactivation for this and other restriction enzymes Note Also available as a FastDigest enzyme for rapid DNA digestion Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes optimized to work in one of the buffers of the Five Buffer System In addition the universal Tango buffer is provided for convenience in double digestions All of the enzymes exhibit 100 activity in the recommended buffer and reaction conditions To ensure consistent performance Thermo Scientific restriction enzyme reaction buffers contain premixed BSA which enhances the stability of many enzymes and binds contaminants that sent in DNA preparations Features• Superior quality stringent quality control and industry leading manufacturing process• Convenient color coded Five Buffer System• Includes universal Tango buffer for double digestions• BSA premixed in reaction buffers• Wide selection of restriction endonuclease specificitiesApplications• Molecular cloning• Restriction site mapping• Genotyping• Southern blotting• Restriction fragment length polymorphism RFLP • SNPNote For methylation sensitivity refer to product specifications
    https://www.bioz.com/result/restriction endonucleases ecori/product/Thermo Fisher
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    restriction endonucleases ecori - by Bioz Stars, 2020-12
    99/100 stars

    Images

    1) Product Images from "A plasmid-based lacZα gene assay for DNA polymerase fidelity measurement"

    Article Title: A plasmid-based lacZα gene assay for DNA polymerase fidelity measurement

    Journal: Analytical Biochemistry

    doi: 10.1016/j.ab.2012.10.019

    The main features of pSJ2 and pSJ3, plasmids useful for measuring DNA polymerase fidelity. (A) The lacZα peptide and lac promoter sequences of M13mp2, pSJ1, pSJ2, and pSJ3. Only the bases actually used in fidelity determination are shown; thus, pSJ1 and pSJ3 contain the lac promoter, but because of the nicking site positions, this element is not used in fidelity assays with these two plasmids. The symbol ∗ indicates identical bases in the lacZα peptide (for all four sequences) and in the lac promoter (for M13mp2 and pSJ2). The underlined sequences are the extra bases at the extremities of pSJ2, necessary for introducing the nicking sites. The ATG triplet that encodes the first methionine in the lacZα peptide is shown, as is the dam methylase (GATC) site altered in pSJ2 and pSJ3. The C shown with the symbol ↓ is the base altered in pSJ2 to introduce a stop codon for expression frequency determination. In all cases, the coding sequence of lacZα is given, which corresponds to the bases on the inner circle of the plasmids illustrated. (B) Plasmid maps of pSJ2 and pSJ3 showing the location and orientation of the lacZα sequences and the nicking endonuclease sites, N(t/b)BbvCI for pSJ2 and N(t/b)Bpu10I for pSJ3. The EcoRI site used for analytical purposes is also shown. The abbreviations dcs and ucs stand for downstream and upstream cutting sites, respectively.
    Figure Legend Snippet: The main features of pSJ2 and pSJ3, plasmids useful for measuring DNA polymerase fidelity. (A) The lacZα peptide and lac promoter sequences of M13mp2, pSJ1, pSJ2, and pSJ3. Only the bases actually used in fidelity determination are shown; thus, pSJ1 and pSJ3 contain the lac promoter, but because of the nicking site positions, this element is not used in fidelity assays with these two plasmids. The symbol ∗ indicates identical bases in the lacZα peptide (for all four sequences) and in the lac promoter (for M13mp2 and pSJ2). The underlined sequences are the extra bases at the extremities of pSJ2, necessary for introducing the nicking sites. The ATG triplet that encodes the first methionine in the lacZα peptide is shown, as is the dam methylase (GATC) site altered in pSJ2 and pSJ3. The C shown with the symbol ↓ is the base altered in pSJ2 to introduce a stop codon for expression frequency determination. In all cases, the coding sequence of lacZα is given, which corresponds to the bases on the inner circle of the plasmids illustrated. (B) Plasmid maps of pSJ2 and pSJ3 showing the location and orientation of the lacZα sequences and the nicking endonuclease sites, N(t/b)BbvCI for pSJ2 and N(t/b)Bpu10I for pSJ3. The EcoRI site used for analytical purposes is also shown. The abbreviations dcs and ucs stand for downstream and upstream cutting sites, respectively.

    Techniques Used: Introduce, Expressing, Sequencing, Plasmid Preparation

    2) Product Images from "Sequence-tagged site-based diagnostic markers linked to a novel anthracnose resistance gene RCt1 in chili pepper (Capsicum annuum L.)"

    Article Title: Sequence-tagged site-based diagnostic markers linked to a novel anthracnose resistance gene RCt1 in chili pepper (Capsicum annuum L.)

    Journal: 3 Biotech

    doi: 10.1007/s13205-018-1552-0

    Southern blot analysis of the four STS markers- CtR-431, CtR-594., CtR-496, and AFLP-376. Restriction enzymes used are EcoRI, HinDIII and XbaI . M, molecular weight marker; R resistant genotype Punjab Lal; S susceptible genotype Arka Lohit. Hybridization
    Figure Legend Snippet: Southern blot analysis of the four STS markers- CtR-431, CtR-594., CtR-496, and AFLP-376. Restriction enzymes used are EcoRI, HinDIII and XbaI . M, molecular weight marker; R resistant genotype Punjab Lal; S susceptible genotype Arka Lohit. Hybridization

    Techniques Used: Southern Blot, Molecular Weight, Marker, Hybridization

    3) Product Images from "Sequence-tagged site-based diagnostic markers linked to a novel anthracnose resistance gene RCt1 in chili pepper (Capsicum annuum L.)"

    Article Title: Sequence-tagged site-based diagnostic markers linked to a novel anthracnose resistance gene RCt1 in chili pepper (Capsicum annuum L.)

    Journal: 3 Biotech

    doi: 10.1007/s13205-018-1552-0

    Southern blot analysis of the four STS markers- CtR-431, CtR-594., CtR-496, and AFLP-376. Restriction enzymes used are EcoRI, HinDIII and XbaI . M, molecular weight marker; R resistant genotype Punjab Lal; S susceptible genotype Arka Lohit. Hybridization
    Figure Legend Snippet: Southern blot analysis of the four STS markers- CtR-431, CtR-594., CtR-496, and AFLP-376. Restriction enzymes used are EcoRI, HinDIII and XbaI . M, molecular weight marker; R resistant genotype Punjab Lal; S susceptible genotype Arka Lohit. Hybridization

    Techniques Used: Southern Blot, Molecular Weight, Marker, Hybridization

    Related Articles

    Construct:

    Article Title: Plasmodium vivax Ookinete Surface Protein Pvs25 Linked to Cholera Toxin B Subunit Induces Potent Transmission-Blocking Immunity by Intranasal as Well as Subcutaneous Immunization ▿
    Article Snippet: .. The fragment was subcloned into the SnaBI and EcoRI sites of the P. pastoris expression vector pPIC9K (Life Technologies, Carlsbad, CA) to construct the plasmid pPvs25H, which was designed to express an α-factor signal-Pvs25-hexahistidine fusion protein (see Fig. ). .. P. pastoris recombination was performed according to the manufacturer's instructions (Life Technologies).

    Purification:

    Article Title: Genome Analysis of Lactobacillus plantarum LL441 and Genetic Characterisation of the Locus for the Lantibiotic Plantaricin C
    Article Snippet: .. Briefly, total genomic DNA was extracted and purified from L. plantarum LL441, and then digested with PstI, EcoRI, or HindIII (Fermentas GmbH, Sankt Leon-Rot, Germany). .. After transferring to a Hybond nylon membrane (Amersham; GE Healthcare, Pittsburgh, PA., United States), the DNA was hybridized with a probe based on an internal segment of the coding sequence of the lanM gene amplified by PCR.

    Generated:

    Article Title: Human Mre11/Human Rad50/Nbs1 and DNA Ligase III?/XRCC1 Protein Complexes Act Together in an Alternative Nonhomologous End Joining Pathway *
    Article Snippet: .. Linear DNA molecules were generated by digestion of pGADT7 (Invitrogen) with BamHI and EcoRI for the four nucleotide 5′ overhangs and of pcDNA4HisMax C (Invitrogen) with KpnI and PstI for the four nucleotide 3′ overhangs. .. Intramolecular joining of the incompatible ends was carried out in 25 m m MOPS (pH 7.0), 60 m m KCl, 0.2% Tween 20, 2 m m DTT, 4 m m MgCl2 , 2 m m MnCl2 , 0.5 m m ATP, 0.8 pmol of plasmid DNA, 10% polyethylene glycol, 0.01 pmol of DNA ligase III/XRCC1 or DNA ligase IV/XRCC4, and 0.06 pmol of hMre11 or MRN as indicated, in a volume of 10 μl.

    Expressing:

    Article Title: Plasmodium vivax Ookinete Surface Protein Pvs25 Linked to Cholera Toxin B Subunit Induces Potent Transmission-Blocking Immunity by Intranasal as Well as Subcutaneous Immunization ▿
    Article Snippet: .. The fragment was subcloned into the SnaBI and EcoRI sites of the P. pastoris expression vector pPIC9K (Life Technologies, Carlsbad, CA) to construct the plasmid pPvs25H, which was designed to express an α-factor signal-Pvs25-hexahistidine fusion protein (see Fig. ). .. P. pastoris recombination was performed according to the manufacturer's instructions (Life Technologies).

    Polymerase Chain Reaction:

    Article Title: Glutamate Activates AMPA Receptor Conductance in the Developing Schwann Cells of the Mammalian Peripheral Nerves
    Article Snippet: .. For restriction analysis, the PCR products from GluA1, GluA2, GluA3, and GluA4 were digested with PstI, SduI, Eco47III, and EcoRI (all from Thermo Scientific), respectively. .. The digestion products were analyzed by agarose gel electrophoresis (stained with GelRel) with the marker pUC19 DNA/MspI (Hpall) (Thermo Scientific).

    Article Title: Allelic Variations of Plasmodium vivax Apical Membrane Antigen-1 (Pv AMA-1) in Malarious Areas of Southeastern Iran Using PCR-RFLP Technique
    Article Snippet: .. RFLP In order to determine the presence of different alleles of Pv AMA-1 gene in the region, PCR–RFLP technique was done to digest the gene using three restriction enzymes EcoR-1, Pvu-II and Hind3 (Thermo cat No #ER0271, #ER0631 and ferments cat No #ER0501 respectively) according to the manufacturer’s recommendations. .. The products were visualized under UV illumination after electrophoresis on 3% agarose gel containing ethidium bromide.

    Plasmid Preparation:

    Article Title: Plasmodium vivax Ookinete Surface Protein Pvs25 Linked to Cholera Toxin B Subunit Induces Potent Transmission-Blocking Immunity by Intranasal as Well as Subcutaneous Immunization ▿
    Article Snippet: .. The fragment was subcloned into the SnaBI and EcoRI sites of the P. pastoris expression vector pPIC9K (Life Technologies, Carlsbad, CA) to construct the plasmid pPvs25H, which was designed to express an α-factor signal-Pvs25-hexahistidine fusion protein (see Fig. ). .. P. pastoris recombination was performed according to the manufacturer's instructions (Life Technologies).

    Article Title: Characterization of diverse homoserine lactone synthases in Escherichia coli
    Article Snippet: .. The Modular Sender Vector and each HSL synthase oligo were cut with EcoRI and XbaI (Thermo Fisher Scientific) and ligated using T4 ligase (New England Biolabs). ..

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  • 99
    Thermo Fisher restriction endonucleases ecori
    Southern blot analysis of the four STS markers- CtR-431, CtR-594., CtR-496, and AFLP-376. Restriction enzymes used are <t>EcoRI,</t> <t>HinDIII</t> and XbaI . M, molecular weight marker; R resistant genotype Punjab Lal; S susceptible genotype Arka Lohit. Hybridization
    Restriction Endonucleases Ecori, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction endonucleases ecori/product/Thermo Fisher
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    restriction endonucleases ecori - by Bioz Stars, 2020-12
    99/100 stars
      Buy from Supplier

    88
    Thermo Fisher restriction endonucleases eco ri
    Southern blot analysis of the four STS markers- CtR-431, CtR-594., CtR-496, and AFLP-376. Restriction enzymes used are <t>EcoRI,</t> <t>HinDIII</t> and XbaI . M, molecular weight marker; R resistant genotype Punjab Lal; S susceptible genotype Arka Lohit. Hybridization
    Restriction Endonucleases Eco Ri, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction endonucleases eco ri/product/Thermo Fisher
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    restriction endonucleases eco ri - by Bioz Stars, 2020-12
    88/100 stars
      Buy from Supplier

    99
    Thermo Fisher restriction endonuclease ecorv
    Southern blot analysis of the four STS markers- CtR-431, CtR-594., CtR-496, and AFLP-376. Restriction enzymes used are <t>EcoRI,</t> <t>HinDIII</t> and XbaI . M, molecular weight marker; R resistant genotype Punjab Lal; S susceptible genotype Arka Lohit. Hybridization
    Restriction Endonuclease Ecorv, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction endonuclease ecorv/product/Thermo Fisher
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    restriction endonuclease ecorv - by Bioz Stars, 2020-12
    99/100 stars
      Buy from Supplier

    Image Search Results


    Southern blot analysis of the four STS markers- CtR-431, CtR-594., CtR-496, and AFLP-376. Restriction enzymes used are EcoRI, HinDIII and XbaI . M, molecular weight marker; R resistant genotype Punjab Lal; S susceptible genotype Arka Lohit. Hybridization

    Journal: 3 Biotech

    Article Title: Sequence-tagged site-based diagnostic markers linked to a novel anthracnose resistance gene RCt1 in chili pepper (Capsicum annuum L.)

    doi: 10.1007/s13205-018-1552-0

    Figure Lengend Snippet: Southern blot analysis of the four STS markers- CtR-431, CtR-594., CtR-496, and AFLP-376. Restriction enzymes used are EcoRI, HinDIII and XbaI . M, molecular weight marker; R resistant genotype Punjab Lal; S susceptible genotype Arka Lohit. Hybridization

    Article Snippet: Three different restriction endonucleases- EcoRI, HinDIII and XbaI (Thermo Fischer Scientific, USA) were used to digest the genomic DNA from the resistant line ‘Punjab Lal’ and the susceptible line ‘Arka Lohit’.

    Techniques: Southern Blot, Molecular Weight, Marker, Hybridization