restriction endonuclease xbai  (New England Biolabs)


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  • 99
    Name:
    Endonuclease V
    Description:
    Endonuclease V 250 units
    Catalog Number:
    m0305s
    Price:
    76
    Category:
    Other Endonucleases
    Size:
    250 units
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    Structured Review

    New England Biolabs restriction endonuclease xbai
    Endonuclease V
    Endonuclease V 250 units
    https://www.bioz.com/result/restriction endonuclease xbai/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    restriction endonuclease xbai - by Bioz Stars, 2021-03
    99/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Functional impacts of 5-hydroxymethylcytosine, 5-formylcytosine, and 5-carboxycytosine at a single hemi-modified CpG dinucleotide in a gene promoter
    Article Snippet: The respective control oligonucleotide without modifications was always ligated in parallel for every independent vector preparation. .. The presence of 5-mC/5-hmC/5-fC/5-caC in the covalently closed vector DNA was verified by the inhibition of cleavage by the AatII restriction endonuclease (NEB). .. Electrophoretic mobility shift assays Vectors pCRE-uno and pCRE-zero were digested with PciI and BmtI (both NEB) to produce the CRE-less 4228 bp fragment and the 180 bp fragment with one (pCRE-uno) or no CRE (pCRE-zero) and cleaned up.

    Article Title: Promoter Methylation Regulates SAMHD1 Gene Expression in Human CD4+ T Cells *
    Article Snippet: Following heat inactivation of the enzyme, the methylated plasmid DNA was purified using DNA Clean and ConcentratorTM -5 (Zymo Research). .. Complete methylation of the pGL4-SAMHD1-luc plasmid was confirmed by digestion with the methylation-sensitive endonuclease HpaII (New England Biolabs), followed by agarose gel electrophoresis. .. Two methods were employed to confirm the methylation status of the SAMHD1 promoter in CD4+ T cells.

    Inhibition:

    Article Title: Functional impacts of 5-hydroxymethylcytosine, 5-formylcytosine, and 5-carboxycytosine at a single hemi-modified CpG dinucleotide in a gene promoter
    Article Snippet: The respective control oligonucleotide without modifications was always ligated in parallel for every independent vector preparation. .. The presence of 5-mC/5-hmC/5-fC/5-caC in the covalently closed vector DNA was verified by the inhibition of cleavage by the AatII restriction endonuclease (NEB). .. Electrophoretic mobility shift assays Vectors pCRE-uno and pCRE-zero were digested with PciI and BmtI (both NEB) to produce the CRE-less 4228 bp fragment and the 180 bp fragment with one (pCRE-uno) or no CRE (pCRE-zero) and cleaned up.

    Purification:

    Article Title: Identification of Dermatophytes by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Analysis of Metalloproteinase-1
    Article Snippet: DNA extraction from the excised gel parts was performed using LaboPass™ Gel (Cosmo, Seoul, Korea) and PCR Clean-up kit (Cosmo) according to the manufacturer's instructions. .. 5) Detection of restriction fragment length polymorphism in the metalloproteinase-1 regions Ten micrograms of purified PCR sample was digested at 65℃ for 16 hours with 10 U of restriction endonuclease BsrD I (New England Biolabs, Ipswich, MA, USA). ..

    Polymerase Chain Reaction:

    Article Title: Identification of Dermatophytes by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Analysis of Metalloproteinase-1
    Article Snippet: DNA extraction from the excised gel parts was performed using LaboPass™ Gel (Cosmo, Seoul, Korea) and PCR Clean-up kit (Cosmo) according to the manufacturer's instructions. .. 5) Detection of restriction fragment length polymorphism in the metalloproteinase-1 regions Ten micrograms of purified PCR sample was digested at 65℃ for 16 hours with 10 U of restriction endonuclease BsrD I (New England Biolabs, Ipswich, MA, USA). ..

    Article Title: Efficient genome editing in hematopoietic stem cells with helper-dependent Ad5/35 vectors expressing site-specific endonucleases under microRNA regulation
    Article Snippet: .. PCR products were hybridized and treated with 2.5 units of T7E1 endonuclease (NEB, Ipswich, MA). .. Digested PCR products were resolved by 10% Tris-borate EDTA buffer (TBE) polyacrylamide gel electrophoresis (Biorad) and stained with ethidium bromide.

    Article Title: Promoter Methylation Regulates SAMHD1 Gene Expression in Human CD4+ T Cells *
    Article Snippet: The PCR products were electrophoresed on a 2% agarose gel and stained with ethidium bromide. .. 2) For HpaII digestion of genomic DNA, followed by SAMHD1 promoter-specific PCR, genomic DNA (1 μg) was digested with 10 units of HpaII endonuclease (New England Biolabs) for 16 h at 37 °C. .. The enzyme was heat-inactivated, and DNA was purified using DNA Clean and ConcentratorTM -5.

    Isolation:

    Article Title: The nicking endonuclease N.BstNBI is closely related to Type IIs restriction endonucleases MlyI and PleI
    Article Snippet: .. The nicking endonuclease N. Bst NBI was originally isolated from the thermophilic bacterium B.stearothermophilus (NEB 928). .. Type IIs restriction endonucleases Ple I and Mly I were isolated from the mesophilic bacteria Pseudomonas lemoignei and Micrococcus lylae , respectively.

    other:

    Article Title: Use of two gRNAs for CRISPR/Cas9 improves bi-allelic homologous recombination efficiency in mouse embryonic stem cells
    Article Snippet: Restriction using T7E1 endonuclease for 25-45 minutes at 37C indicated the presence of mismatches in the rehybridized products.

    Methylation:

    Article Title: Promoter Methylation Regulates SAMHD1 Gene Expression in Human CD4+ T Cells *
    Article Snippet: Following heat inactivation of the enzyme, the methylated plasmid DNA was purified using DNA Clean and ConcentratorTM -5 (Zymo Research). .. Complete methylation of the pGL4-SAMHD1-luc plasmid was confirmed by digestion with the methylation-sensitive endonuclease HpaII (New England Biolabs), followed by agarose gel electrophoresis. .. Two methods were employed to confirm the methylation status of the SAMHD1 promoter in CD4+ T cells.

    Agarose Gel Electrophoresis:

    Article Title: Promoter Methylation Regulates SAMHD1 Gene Expression in Human CD4+ T Cells *
    Article Snippet: Following heat inactivation of the enzyme, the methylated plasmid DNA was purified using DNA Clean and ConcentratorTM -5 (Zymo Research). .. Complete methylation of the pGL4-SAMHD1-luc plasmid was confirmed by digestion with the methylation-sensitive endonuclease HpaII (New England Biolabs), followed by agarose gel electrophoresis. .. Two methods were employed to confirm the methylation status of the SAMHD1 promoter in CD4+ T cells.

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    New England Biolabs restriction endonucleases xbai
    (A) <t>PFGE</t> analysis of S . Typhimurium DT104 using <t>XbaI</t> and AvrII. Isolate groups were color identified for simplicity as follows, and the color codes were maintained in all of the assays: reference strain (white); group A, environmental (green); group B,
    Restriction Endonucleases Xbai, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction endonucleases xbai/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    restriction endonucleases xbai - by Bioz Stars, 2021-03
    99/100 stars
      Buy from Supplier

    99
    New England Biolabs restriction endonuclease xbai
    (A) <t>PFGE</t> analysis of S . Typhimurium DT104 using <t>XbaI</t> and AvrII. Isolate groups were color identified for simplicity as follows, and the color codes were maintained in all of the assays: reference strain (white); group A, environmental (green); group B,
    Restriction Endonuclease Xbai, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction endonuclease xbai/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    restriction endonuclease xbai - by Bioz Stars, 2021-03
    99/100 stars
      Buy from Supplier

    Image Search Results


    (A) PFGE analysis of S . Typhimurium DT104 using XbaI and AvrII. Isolate groups were color identified for simplicity as follows, and the color codes were maintained in all of the assays: reference strain (white); group A, environmental (green); group B,

    Journal: Applied and Environmental Microbiology

    Article Title: Evidence of Metabolic Switching and Implications for Food Safety from the Phenome(s) of Salmonella enterica Serovar Typhimurium DT104 Cultured at Selected Points across the Pork Production Food Chain

    doi: 10.1128/AEM.01041-13

    Figure Lengend Snippet: (A) PFGE analysis of S . Typhimurium DT104 using XbaI and AvrII. Isolate groups were color identified for simplicity as follows, and the color codes were maintained in all of the assays: reference strain (white); group A, environmental (green); group B,

    Article Snippet: Pulsed-field gel electrophoresis (PFGE) was performed using the restriction endonucleases XbaI and AvrII (New England BioLabs) by following the CDC PulseNet protocol ( ).

    Techniques: