restriction endonuclease xbai (New England Biolabs)


Name:
Endonuclease V
Description:
Endonuclease V 250 units
Catalog Number:
m0305s
Price:
76
Category:
Other Endonucleases
Size:
250 units
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Endonuclease V 250 units
https://www.bioz.com/result/restriction endonuclease xbai/product/New England Biolabs
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99
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Plasmid Preparation:Article Title: Functional impacts of 5-hydroxymethylcytosine, 5-formylcytosine, and 5-carboxycytosine at a single hemi-modified CpG dinucleotide in a gene promoter Article Snippet: The respective control oligonucleotide without modifications was always ligated in parallel for every independent vector preparation. .. The presence of 5-mC/5-hmC/5-fC/5-caC in the covalently closed vector DNA was verified by the inhibition of cleavage by the Article Title: Promoter Methylation Regulates SAMHD1 Gene Expression in Human CD4+ T Cells * Article Snippet: Following heat inactivation of the enzyme, the methylated plasmid DNA was purified using DNA Clean and ConcentratorTM -5 (Zymo Research). .. Complete methylation of the pGL4-SAMHD1-luc plasmid was confirmed by digestion with the Inhibition:Article Title: Functional impacts of 5-hydroxymethylcytosine, 5-formylcytosine, and 5-carboxycytosine at a single hemi-modified CpG dinucleotide in a gene promoter Article Snippet: The respective control oligonucleotide without modifications was always ligated in parallel for every independent vector preparation. .. The presence of 5-mC/5-hmC/5-fC/5-caC in the covalently closed vector DNA was verified by the inhibition of cleavage by the Purification:Article Title: Identification of Dermatophytes by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Analysis of Metalloproteinase-1 Article Snippet: DNA extraction from the excised gel parts was performed using LaboPass™ Gel (Cosmo, Seoul, Korea) and PCR Clean-up kit (Cosmo) according to the manufacturer's instructions. .. 5) Detection of restriction fragment length polymorphism in the metalloproteinase-1 regions Ten micrograms of purified PCR sample was digested at 65℃ for 16 hours with 10 U of Polymerase Chain Reaction:Article Title: Identification of Dermatophytes by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Analysis of Metalloproteinase-1 Article Snippet: DNA extraction from the excised gel parts was performed using LaboPass™ Gel (Cosmo, Seoul, Korea) and PCR Clean-up kit (Cosmo) according to the manufacturer's instructions. .. 5) Detection of restriction fragment length polymorphism in the metalloproteinase-1 regions Ten micrograms of purified PCR sample was digested at 65℃ for 16 hours with 10 U of Article Title: Efficient genome editing in hematopoietic stem cells with helper-dependent Ad5/35 vectors expressing site-specific endonucleases under microRNA regulation Article Snippet: .. PCR products were hybridized and treated with 2.5 units of Article Title: Promoter Methylation Regulates SAMHD1 Gene Expression in Human CD4+ T Cells * Article Snippet: The PCR products were electrophoresed on a 2% agarose gel and stained with ethidium bromide. .. 2) For HpaII digestion of genomic DNA, followed by SAMHD1 promoter-specific PCR, genomic DNA (1 μg) was digested with 10 units of Isolation:Article Title: The nicking endonuclease N.BstNBI is closely related to Type IIs restriction endonucleases MlyI and PleI Article Snippet: .. The nicking other:Article Title: Use of two gRNAs for CRISPR/Cas9 improves bi-allelic homologous recombination efficiency in mouse embryonic stem cells Article Snippet: Restriction using T7E1 endonuclease for 25-45 minutes at 37C indicated the presence of mismatches in the rehybridized products. Methylation:Article Title: Promoter Methylation Regulates SAMHD1 Gene Expression in Human CD4+ T Cells * Article Snippet: Following heat inactivation of the enzyme, the methylated plasmid DNA was purified using DNA Clean and ConcentratorTM -5 (Zymo Research). .. Complete methylation of the pGL4-SAMHD1-luc plasmid was confirmed by digestion with the Agarose Gel Electrophoresis:Article Title: Promoter Methylation Regulates SAMHD1 Gene Expression in Human CD4+ T Cells * Article Snippet: Following heat inactivation of the enzyme, the methylated plasmid DNA was purified using DNA Clean and ConcentratorTM -5 (Zymo Research). .. Complete methylation of the pGL4-SAMHD1-luc plasmid was confirmed by digestion with the |