restriction endonuclease mspi  (New England Biolabs)


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    Name:
    Endonuclease V
    Description:
    Endonuclease V 250 units
    Catalog Number:
    m0305s
    Price:
    76
    Size:
    250 units
    Category:
    Other Endonucleases
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    Structured Review

    New England Biolabs restriction endonuclease mspi
    Endonuclease V
    Endonuclease V 250 units
    https://www.bioz.com/result/restriction endonuclease mspi/product/New England Biolabs
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    restriction endonuclease mspi - by Bioz Stars, 2020-07
    99/100 stars

    Images

    1) Product Images from "Changing Feeding Regimes To Demonstrate Flexible Biogas Production: Effects on Process Performance, Microbial Community Structure, and Methanogenesis Pathways"

    Article Title: Changing Feeding Regimes To Demonstrate Flexible Biogas Production: Effects on Process Performance, Microbial Community Structure, and Methanogenesis Pathways

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.02320-15

    Nonmetric multidimensional scaling (nMDS) ordination of bacterial community T-RFLP data for 14 samples collected from two DDGS-fed reactors (reactors A and B) over phases I to IV with HaeIII (A) and MspI (B) restriction enzyme digestion. Statistically
    Figure Legend Snippet: Nonmetric multidimensional scaling (nMDS) ordination of bacterial community T-RFLP data for 14 samples collected from two DDGS-fed reactors (reactors A and B) over phases I to IV with HaeIII (A) and MspI (B) restriction enzyme digestion. Statistically

    Techniques Used:

    Related Articles

    Agarose Gel Electrophoresis:

    Article Title: Upregulation of KLHDC4 Predicts a Poor Prognosis in Human Nasopharyngeal Carcinoma
    Article Snippet: .. The mixture of duplexes was treated with T7E1 endonuclease for 20 minutes at 37°C (New England Biolabs); the reaction was stopped using 1.5 μl of 0.25 M EDTA, and the products were analyzed on a 3% agarose gel. .. Establishment of a KLHDC4 knockout cell line CNE2 cells were cultured in 6-well dishes to 70–80% confluence and then cotransfected with 1 μg of KLHDC4 sgRNA#2 plasmid plus 1 μg of pSpCas9(BB)-2A-GFP plasmid and 5 μl of Lipofectamine 2000 per well.

    Methylation:

    Article Title: Transcriptome analysis of an apple (Malus × domestica) yellow fruit somatic mutation identifies a gene network module highly associated with anthocyanin and epigenetic regulation
    Article Snippet: .. One microgram (µg) of genomic DNA (g DNA) isolated from KID and BLO fruit skin samples collected in 2013 and 2014 were digested with methylation-specific endonuclease enzyme McrBC (New England Biolabs), according to the manufacturer’s instructions, with three biological replicates. .. For the negative control, Guanosine-5′-triphosphate (GTP) was replaced by water in the reaction.

    Isolation:

    Article Title: Transcriptome analysis of an apple (Malus × domestica) yellow fruit somatic mutation identifies a gene network module highly associated with anthocyanin and epigenetic regulation
    Article Snippet: .. One microgram (µg) of genomic DNA (g DNA) isolated from KID and BLO fruit skin samples collected in 2013 and 2014 were digested with methylation-specific endonuclease enzyme McrBC (New England Biolabs), according to the manufacturer’s instructions, with three biological replicates. .. For the negative control, Guanosine-5′-triphosphate (GTP) was replaced by water in the reaction.

    Purification:

    Article Title: Solution parameters modulating DNA binding specificity of the restriction endonuclease EcoRV
    Article Snippet: .. DNA binding and cleavage experiments were performed with highly purified EcoRV restriction endonuclease (described below) or with commercial EcoRV sample purchased from New England Biolabs. .. Active protein concentrations of the EcoRV were determined by the direct titration with the specific site 310 bp DNA fragment under conditions of stoichiometric binding as described previously [ , ].

    Polymerase Chain Reaction:

    Article Title: Efficient genome editing in hematopoietic stem cells with helper-dependent Ad5/35 vectors expressing site-specific endonucleases under microRNA regulation
    Article Snippet: .. PCR products were hybridized and treated with 2.5 units of T7E1 endonuclease (NEB, Ipswich, MA). .. Digested PCR products were resolved by 10% Tris-borate EDTA buffer (TBE) polyacrylamide gel electrophoresis (Biorad) and stained with ethidium bromide.

    Article Title: Promoter Methylation Regulates SAMHD1 Gene Expression in Human CD4+ T Cells *
    Article Snippet: .. 2) For HpaII digestion of genomic DNA, followed by SAMHD1 promoter-specific PCR, genomic DNA (1 μg) was digested with 10 units of HpaII endonuclease (New England Biolabs) for 16 h at 37 °C. .. The enzyme was heat-inactivated, and DNA was purified using DNA Clean and ConcentratorTM -5.

    Sonication:

    Article Title: Dependence of antibody gene diversification on uracil excision
    Article Snippet: .. Total cell extracts of vertebrate cells were prepared by sonication and samples (2–20 μg of protein) assayed for their ability to excise uracil from a FITC-labeled oligonucleotide substrate essentially as previously described , except that 0.2 U of apyrimidinic endonuclease (New England Biolabs) was included in each reaction. .. When measuring SMUG1 activity, the endogenous UNG enzyme (if present) was inhibited using 0.5 U of Ugi (New England Biolabs, Inc.) where indicated.

    Binding Assay:

    Article Title: Solution parameters modulating DNA binding specificity of the restriction endonuclease EcoRV
    Article Snippet: .. DNA binding and cleavage experiments were performed with highly purified EcoRV restriction endonuclease (described below) or with commercial EcoRV sample purchased from New England Biolabs. .. Active protein concentrations of the EcoRV were determined by the direct titration with the specific site 310 bp DNA fragment under conditions of stoichiometric binding as described previously [ , ].

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    New England Biolabs mspi restriction endonucleases
    Control lambda <t>DNA</t> analysis. a Ethidium bromide-staining agarose gel showing unmethylated and methylated lambda DNAs digested with HpaII and <t>MspI.</t> ND: lambda DNA not digested. b GDMI values of unmethylated and methylated lambda DNAs. The values represent the main of three independent triplicate experiments with standard error mean
    Mspi Restriction Endonucleases, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mspi restriction endonucleases/product/New England Biolabs
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    mspi restriction endonucleases - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    99
    New England Biolabs restriction endonuclease mspi
    Nonmetric multidimensional scaling (nMDS) ordination of bacterial community T-RFLP data for 14 samples collected from two DDGS-fed reactors (reactors A and B) over phases I to IV with <t>HaeIII</t> (A) and <t>MspI</t> (B) restriction enzyme digestion. Statistically
    Restriction Endonuclease Mspi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction endonuclease mspi/product/New England Biolabs
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    restriction endonuclease mspi - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    Image Search Results


    Control lambda DNA analysis. a Ethidium bromide-staining agarose gel showing unmethylated and methylated lambda DNAs digested with HpaII and MspI. ND: lambda DNA not digested. b GDMI values of unmethylated and methylated lambda DNAs. The values represent the main of three independent triplicate experiments with standard error mean

    Journal: Age

    Article Title: Global DNA methylation in old subjects is correlated with frailty

    doi: 10.1007/s11357-011-9216-6

    Figure Lengend Snippet: Control lambda DNA analysis. a Ethidium bromide-staining agarose gel showing unmethylated and methylated lambda DNAs digested with HpaII and MspI. ND: lambda DNA not digested. b GDMI values of unmethylated and methylated lambda DNAs. The values represent the main of three independent triplicate experiments with standard error mean

    Article Snippet: One hundred nanograms of methylated and unmethylated lambda DNA were separately incubated with 5 U of HpaII and MspI restriction endonucleases (New England Biolabs) at 37°C overnight and successively at 65°C for 20 min to inactivate the endonucleases.

    Techniques: Lambda DNA Preparation, Staining, Agarose Gel Electrophoresis, Methylation

    Control human DNA analysis. a Ethidium bromide-staining agarose gel showing unmethylated and methylated human DNAs and a mixture of equal amount of unmethylated and methylated human DNAs digested with HpaII and MspI. ND human DNA not digested. b GDMI values of unmethylated, methylated and the mixture of unmethylated and methylated human DNAs. The values represent the main of three independent triplicate experiments with standard error mean

    Journal: Age

    Article Title: Global DNA methylation in old subjects is correlated with frailty

    doi: 10.1007/s11357-011-9216-6

    Figure Lengend Snippet: Control human DNA analysis. a Ethidium bromide-staining agarose gel showing unmethylated and methylated human DNAs and a mixture of equal amount of unmethylated and methylated human DNAs digested with HpaII and MspI. ND human DNA not digested. b GDMI values of unmethylated, methylated and the mixture of unmethylated and methylated human DNAs. The values represent the main of three independent triplicate experiments with standard error mean

    Article Snippet: One hundred nanograms of methylated and unmethylated lambda DNA were separately incubated with 5 U of HpaII and MspI restriction endonucleases (New England Biolabs) at 37°C overnight and successively at 65°C for 20 min to inactivate the endonucleases.

    Techniques: Staining, Agarose Gel Electrophoresis, Methylation

    Nonmetric multidimensional scaling (nMDS) ordination of bacterial community T-RFLP data for 14 samples collected from two DDGS-fed reactors (reactors A and B) over phases I to IV with HaeIII (A) and MspI (B) restriction enzyme digestion. Statistically

    Journal: Applied and Environmental Microbiology

    Article Title: Changing Feeding Regimes To Demonstrate Flexible Biogas Production: Effects on Process Performance, Microbial Community Structure, and Methanogenesis Pathways

    doi: 10.1128/AEM.02320-15

    Figure Lengend Snippet: Nonmetric multidimensional scaling (nMDS) ordination of bacterial community T-RFLP data for 14 samples collected from two DDGS-fed reactors (reactors A and B) over phases I to IV with HaeIII (A) and MspI (B) restriction enzyme digestion. Statistically

    Article Snippet: Briefly, bacterial 16S rRNA genes were subjected to restriction enzyme digestion with either the restriction endonuclease HaeIII or the restriction endonuclease MspI (New England BioLabs, Schwalbach, Germany), whereas mcrA genes were digested with BstNI (New England BioLabs, Schwalbach, Germany).

    Techniques: