restriction endonuclease fsp i  (New England Biolabs)


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    Structured Review

    New England Biolabs restriction endonuclease fsp i
    Agarose gel electrophoresis of KS7-KS8 <t>PCR</t> products digested with <t>Fsp</t> I (A) or Hha I (B) and of PCR amplification products with primers Stx1c-1 and Stx1c-2 (C). M, molecular weight marker (1-kb DNA ladder; Gibco BRL, Eggenstein, Germany). In lanes 1 to 12, the following STEC strains (genotypes, serotypes, and origins, if not human, in parentheses) are shown: 1, 808/97 ( stx 1c + stx 2d ; ONT:H8); 2, 3115/97 ( stx 1c + stx 2d ; O128:H2); 3, 521/99 ( stx 1c + stx 2d ; O rough :H19); 4, 4756/98 ( stx 1c + stx 2d ; O70:H − ); 5, 273/00 ( stx 1c + stx 2d ; O128:H − ; sheep); 6, 295/00 ( stx 1c + stx 2d ; O128:H − ; sheep); 7, EDL 933 ( stx 1 + stx 2 ; O157:H7); 8, 2544/00 ( stx 1 ; O145:H − ); 9, 3385/00 ( stx 1 + stx 2 ; O111:H − ); 10, 4424/99 ( stx 1 + stx 2 ; O157:H7); 11, 2049/98 ( stx 1 + stx 2c ; O157:H − ); 12, 2050/98 ( stx 1 + stx 2 + stx 2c ; O157:H − ).
    Restriction Endonuclease Fsp I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction endonuclease fsp i/product/New England Biolabs
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    restriction endonuclease fsp i - by Bioz Stars, 2020-03
    79/100 stars

    Related Products / Commonly Used Together

    pcr
    stxb 1 gene
    primers ks7-ks8
    hha i

    Images

    1) Product Images from "Identification, Characterization, and Distribution of a Shiga Toxin 1 Gene Variant (stx1c) in Escherichia coli Strains Isolated from Humans"

    Article Title: Identification, Characterization, and Distribution of a Shiga Toxin 1 Gene Variant (stx1c) in Escherichia coli Strains Isolated from Humans

    Journal: Journal of Clinical Microbiology

    doi: 10.1128/JCM.40.4.1441-1446.2002

    Agarose gel electrophoresis of KS7-KS8 PCR products digested with Fsp I (A) or Hha I (B) and of PCR amplification products with primers Stx1c-1 and Stx1c-2 (C). M, molecular weight marker (1-kb DNA ladder; Gibco BRL, Eggenstein, Germany). In lanes 1 to 12, the following STEC strains (genotypes, serotypes, and origins, if not human, in parentheses) are shown: 1, 808/97 ( stx 1c + stx 2d ; ONT:H8); 2, 3115/97 ( stx 1c + stx 2d ; O128:H2); 3, 521/99 ( stx 1c + stx 2d ; O rough :H19); 4, 4756/98 ( stx 1c + stx 2d ; O70:H − ); 5, 273/00 ( stx 1c + stx 2d ; O128:H − ; sheep); 6, 295/00 ( stx 1c + stx 2d ; O128:H − ; sheep); 7, EDL 933 ( stx 1 + stx 2 ; O157:H7); 8, 2544/00 ( stx 1 ; O145:H − ); 9, 3385/00 ( stx 1 + stx 2 ; O111:H − ); 10, 4424/99 ( stx 1 + stx 2 ; O157:H7); 11, 2049/98 ( stx 1 + stx 2c ; O157:H − ); 12, 2050/98 ( stx 1 + stx 2 + stx 2c ; O157:H − ).
    Figure Legend Snippet: Agarose gel electrophoresis of KS7-KS8 PCR products digested with Fsp I (A) or Hha I (B) and of PCR amplification products with primers Stx1c-1 and Stx1c-2 (C). M, molecular weight marker (1-kb DNA ladder; Gibco BRL, Eggenstein, Germany). In lanes 1 to 12, the following STEC strains (genotypes, serotypes, and origins, if not human, in parentheses) are shown: 1, 808/97 ( stx 1c + stx 2d ; ONT:H8); 2, 3115/97 ( stx 1c + stx 2d ; O128:H2); 3, 521/99 ( stx 1c + stx 2d ; O rough :H19); 4, 4756/98 ( stx 1c + stx 2d ; O70:H − ); 5, 273/00 ( stx 1c + stx 2d ; O128:H − ; sheep); 6, 295/00 ( stx 1c + stx 2d ; O128:H − ; sheep); 7, EDL 933 ( stx 1 + stx 2 ; O157:H7); 8, 2544/00 ( stx 1 ; O145:H − ); 9, 3385/00 ( stx 1 + stx 2 ; O111:H − ); 10, 4424/99 ( stx 1 + stx 2 ; O157:H7); 11, 2049/98 ( stx 1 + stx 2c ; O157:H − ); 12, 2050/98 ( stx 1 + stx 2 + stx 2c ; O157:H − ).

    Techniques Used: Agarose Gel Electrophoresis, Polymerase Chain Reaction, Amplification, Molecular Weight, Marker

    Related Articles

    Amplification:

    Article Title: Identification, Characterization, and Distribution of a Shiga Toxin 1 Gene Variant (stx1c) in Escherichia coli Strains Isolated from Humans
    Article Snippet: .. The stxB 1 gene was amplified with primers KS7-KS8 (Table ), and 12 μl of each PCR product was digested with restriction endonuclease Fsp I or Hha I (New England BioLabs GmbH, Frankfurt, Germany), as recommended by the manufacturer. .. Restriction fragments were separated on a 2% (wt/vol) agarose gel and visualized by staining with ethidium bromide.

    Agarose Gel Electrophoresis:

    Article Title: Identification, Characterization, and Distribution of a Shiga Toxin 1 Gene Variant (stx1c) in Escherichia coli Strains Isolated from Humans
    Article Snippet: The stxB 1 gene was amplified with primers KS7-KS8 (Table ), and 12 μl of each PCR product was digested with restriction endonuclease Fsp I or Hha I (New England BioLabs GmbH, Frankfurt, Germany), as recommended by the manufacturer. .. Restriction fragments were separated on a 2% (wt/vol) agarose gel and visualized by staining with ethidium bromide.

    Polymerase Chain Reaction:

    Article Title: Identification, Characterization, and Distribution of a Shiga Toxin 1 Gene Variant (stx1c) in Escherichia coli Strains Isolated from Humans
    Article Snippet: .. The stxB 1 gene was amplified with primers KS7-KS8 (Table ), and 12 μl of each PCR product was digested with restriction endonuclease Fsp I or Hha I (New England BioLabs GmbH, Frankfurt, Germany), as recommended by the manufacturer. .. Restriction fragments were separated on a 2% (wt/vol) agarose gel and visualized by staining with ethidium bromide.

    Sequencing:

    Article Title: Identification, Characterization, and Distribution of a Shiga Toxin 1 Gene Variant (stx1c) in Escherichia coli Strains Isolated from Humans
    Article Snippet: The stxB 1 gene was amplified with primers KS7-KS8 (Table ), and 12 μl of each PCR product was digested with restriction endonuclease Fsp I or Hha I (New England BioLabs GmbH, Frankfurt, Germany), as recommended by the manufacturer. .. The restriction enzymes for the differentiation of classical stx 1 and the stx 1c variant were selected with the DNASIS program, version 2.0, from Hitachi Software (San Bruno, Calif.) based on the published sequence of stx 1 from phage 933J (accession no. ) and the sequence of stx 1c from E. coli strain 3115/97 determined in this study.

    Staining:

    Article Title: Identification, Characterization, and Distribution of a Shiga Toxin 1 Gene Variant (stx1c) in Escherichia coli Strains Isolated from Humans
    Article Snippet: The stxB 1 gene was amplified with primers KS7-KS8 (Table ), and 12 μl of each PCR product was digested with restriction endonuclease Fsp I or Hha I (New England BioLabs GmbH, Frankfurt, Germany), as recommended by the manufacturer. .. Restriction fragments were separated on a 2% (wt/vol) agarose gel and visualized by staining with ethidium bromide.

    Variant Assay:

    Article Title: Identification, Characterization, and Distribution of a Shiga Toxin 1 Gene Variant (stx1c) in Escherichia coli Strains Isolated from Humans
    Article Snippet: The stxB 1 gene was amplified with primers KS7-KS8 (Table ), and 12 μl of each PCR product was digested with restriction endonuclease Fsp I or Hha I (New England BioLabs GmbH, Frankfurt, Germany), as recommended by the manufacturer. .. The restriction enzymes for the differentiation of classical stx 1 and the stx 1c variant were selected with the DNASIS program, version 2.0, from Hitachi Software (San Bruno, Calif.) based on the published sequence of stx 1 from phage 933J (accession no. ) and the sequence of stx 1c from E. coli strain 3115/97 determined in this study.

    Software:

    Article Title: Identification, Characterization, and Distribution of a Shiga Toxin 1 Gene Variant (stx1c) in Escherichia coli Strains Isolated from Humans
    Article Snippet: The stxB 1 gene was amplified with primers KS7-KS8 (Table ), and 12 μl of each PCR product was digested with restriction endonuclease Fsp I or Hha I (New England BioLabs GmbH, Frankfurt, Germany), as recommended by the manufacturer. .. The restriction enzymes for the differentiation of classical stx 1 and the stx 1c variant were selected with the DNASIS program, version 2.0, from Hitachi Software (San Bruno, Calif.) based on the published sequence of stx 1 from phage 933J (accession no. ) and the sequence of stx 1c from E. coli strain 3115/97 determined in this study.

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  • 79
    New England Biolabs restriction endonuclease fsp i
    Agarose gel electrophoresis of KS7-KS8 <t>PCR</t> products digested with <t>Fsp</t> I (A) or Hha I (B) and of PCR amplification products with primers Stx1c-1 and Stx1c-2 (C). M, molecular weight marker (1-kb DNA ladder; Gibco BRL, Eggenstein, Germany). In lanes 1 to 12, the following STEC strains (genotypes, serotypes, and origins, if not human, in parentheses) are shown: 1, 808/97 ( stx 1c + stx 2d ; ONT:H8); 2, 3115/97 ( stx 1c + stx 2d ; O128:H2); 3, 521/99 ( stx 1c + stx 2d ; O rough :H19); 4, 4756/98 ( stx 1c + stx 2d ; O70:H − ); 5, 273/00 ( stx 1c + stx 2d ; O128:H − ; sheep); 6, 295/00 ( stx 1c + stx 2d ; O128:H − ; sheep); 7, EDL 933 ( stx 1 + stx 2 ; O157:H7); 8, 2544/00 ( stx 1 ; O145:H − ); 9, 3385/00 ( stx 1 + stx 2 ; O111:H − ); 10, 4424/99 ( stx 1 + stx 2 ; O157:H7); 11, 2049/98 ( stx 1 + stx 2c ; O157:H − ); 12, 2050/98 ( stx 1 + stx 2 + stx 2c ; O157:H − ).
    Restriction Endonuclease Fsp I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction endonuclease fsp i/product/New England Biolabs
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    restriction endonuclease fsp i - by Bioz Stars, 2020-03
    79/100 stars
      Buy from Supplier

    Image Search Results


    Agarose gel electrophoresis of KS7-KS8 PCR products digested with Fsp I (A) or Hha I (B) and of PCR amplification products with primers Stx1c-1 and Stx1c-2 (C). M, molecular weight marker (1-kb DNA ladder; Gibco BRL, Eggenstein, Germany). In lanes 1 to 12, the following STEC strains (genotypes, serotypes, and origins, if not human, in parentheses) are shown: 1, 808/97 ( stx 1c + stx 2d ; ONT:H8); 2, 3115/97 ( stx 1c + stx 2d ; O128:H2); 3, 521/99 ( stx 1c + stx 2d ; O rough :H19); 4, 4756/98 ( stx 1c + stx 2d ; O70:H − ); 5, 273/00 ( stx 1c + stx 2d ; O128:H − ; sheep); 6, 295/00 ( stx 1c + stx 2d ; O128:H − ; sheep); 7, EDL 933 ( stx 1 + stx 2 ; O157:H7); 8, 2544/00 ( stx 1 ; O145:H − ); 9, 3385/00 ( stx 1 + stx 2 ; O111:H − ); 10, 4424/99 ( stx 1 + stx 2 ; O157:H7); 11, 2049/98 ( stx 1 + stx 2c ; O157:H − ); 12, 2050/98 ( stx 1 + stx 2 + stx 2c ; O157:H − ).

    Journal: Journal of Clinical Microbiology

    Article Title: Identification, Characterization, and Distribution of a Shiga Toxin 1 Gene Variant (stx1c) in Escherichia coli Strains Isolated from Humans

    doi: 10.1128/JCM.40.4.1441-1446.2002

    Figure Lengend Snippet: Agarose gel electrophoresis of KS7-KS8 PCR products digested with Fsp I (A) or Hha I (B) and of PCR amplification products with primers Stx1c-1 and Stx1c-2 (C). M, molecular weight marker (1-kb DNA ladder; Gibco BRL, Eggenstein, Germany). In lanes 1 to 12, the following STEC strains (genotypes, serotypes, and origins, if not human, in parentheses) are shown: 1, 808/97 ( stx 1c + stx 2d ; ONT:H8); 2, 3115/97 ( stx 1c + stx 2d ; O128:H2); 3, 521/99 ( stx 1c + stx 2d ; O rough :H19); 4, 4756/98 ( stx 1c + stx 2d ; O70:H − ); 5, 273/00 ( stx 1c + stx 2d ; O128:H − ; sheep); 6, 295/00 ( stx 1c + stx 2d ; O128:H − ; sheep); 7, EDL 933 ( stx 1 + stx 2 ; O157:H7); 8, 2544/00 ( stx 1 ; O145:H − ); 9, 3385/00 ( stx 1 + stx 2 ; O111:H − ); 10, 4424/99 ( stx 1 + stx 2 ; O157:H7); 11, 2049/98 ( stx 1 + stx 2c ; O157:H − ); 12, 2050/98 ( stx 1 + stx 2 + stx 2c ; O157:H − ).

    Article Snippet: The stxB 1 gene was amplified with primers KS7-KS8 (Table ), and 12 μl of each PCR product was digested with restriction endonuclease Fsp I or Hha I (New England BioLabs GmbH, Frankfurt, Germany), as recommended by the manufacturer.

    Techniques: Agarose Gel Electrophoresis, Polymerase Chain Reaction, Amplification, Molecular Weight, Marker