restriction endonuclease ecorv  (Thermo Fisher)


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    Name:
    Endonuclease V
    Description:
    Thermo Scientific Endonuclease V T maritima Endo V is a 3 endonuclease involved in DNA repair which initiates removal of deaminated bases from damaged DNA including uracil hypoxanthine and xanthine Endonuclease V is also active toward abasic sites and urea sites base pair mismatches flap and pseudo Y structures and small insertions deletions in DNA molecules The cleavage site generated by Endonuclease V is at the second phosphodiester bond 3 to a lesion Highlights• Optimal activity at temperatures of 65 to 70°CApplications• High throughput methods for mutation research• Studies in mutagenesis and DNA repair• Mismatch cleavage• GenotypingNoteUse of this enzyme in certain applications may be covered by patents and may require a license When the enzyme is in excess the primary nicked products experience a second nicking event on the complementary strand leading to a double stranded break At low concentrations however Endonuclease V first nicks a DNA strand at the lesions located closer to the 5 end of DNA molecule Single stranded DNA is cleaved with much lower efficiency Mg2 or Mn2 ions are required for enzyme activity
    Catalog Number:
    en0141
    Price:
    None
    Applications:
    Genotyping & Genomic Profiling|Rare & Low-Frequency Mutation Analysis|Gene Expression Analysis & Genotyping
    Category:
    Proteins Enzymes Peptides
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    Structured Review

    Thermo Fisher restriction endonuclease ecorv
    Thermo Scientific Endonuclease V T maritima Endo V is a 3 endonuclease involved in DNA repair which initiates removal of deaminated bases from damaged DNA including uracil hypoxanthine and xanthine Endonuclease V is also active toward abasic sites and urea sites base pair mismatches flap and pseudo Y structures and small insertions deletions in DNA molecules The cleavage site generated by Endonuclease V is at the second phosphodiester bond 3 to a lesion Highlights• Optimal activity at temperatures of 65 to 70°CApplications• High throughput methods for mutation research• Studies in mutagenesis and DNA repair• Mismatch cleavage• GenotypingNoteUse of this enzyme in certain applications may be covered by patents and may require a license When the enzyme is in excess the primary nicked products experience a second nicking event on the complementary strand leading to a double stranded break At low concentrations however Endonuclease V first nicks a DNA strand at the lesions located closer to the 5 end of DNA molecule Single stranded DNA is cleaved with much lower efficiency Mg2 or Mn2 ions are required for enzyme activity
    https://www.bioz.com/result/restriction endonuclease ecorv/product/Thermo Fisher
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    restriction endonuclease ecorv - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Evolutionary Story of a Satellite DNA from Phodopus sungorus (Rodentia, Cricetidae)
    Article Snippet: .. Isolation, Cloning, and Sequencing of PSUcentSat Sequence PSU genomic DNA was digested with the restriction endonuclease (RE) Mbo I, according to the manufacturers’ instructions (Invitrogen Life Technologies), resulting in a smear with DNA fragments ranging between 3 kb and 100 bp. .. The restriction products were later cloned using routine procedures (FERMENTAS Life Science, Invitrogen Life Technologies).

    Transfection:

    Article Title: HMGB1 released by irradiated tumor cells promotes living tumor cell proliferation via paracrine effect
    Article Snippet: .. In detail, 293 T cells (1 × 106 cells per well; 6-well plate) were transfected with lentiviral construct expressing two gRNAs (gRNA1: 5′ GGAAGAGTCGACTCGCTT 3′, gRNA2: 5′ GTGATGTTGCGAAGAAAC 3′) and Cas9 endonuclease together with ecotropic packaging plasmids, using Lipofectamine 2000 (Invitrogen, Carlsbad, CA) according to manufacturer’s instructions. .. Media containing viruses were collected 48 h after transfection and then utilized to infect HeLa and HT29 cells.

    Amplification:

    Article Title: Entamoeba moshkovskii Infections in Children in Bangladesh
    Article Snippet: .. E. moshkovskii –specific nested SSU-rDNA gene amplification products were digested with restriction endonuclease Xho I for 1 h at 37°C according to the manufacturer’s instructions (Invitrogen Corp., Carlsbad, CA) to verify species identity. .. All PCR products were separated in 1.8% NuSieve 3:1 agarose gels (Flowgen, Lichfield, England) in 1x Tris-borate-EDTA buffer and visualized after staining with ethidium bromide (0.2 µg mL-1 ; Sigma-Aldrich Co. Ltd, Poole, England).

    Mutagenesis:

    Article Title: Comparison of Zebrafish tmem88a mutant and morpholino knockdown phenotypes
    Article Snippet: .. In addition, cDNA generated from tmem88a mutants is not digested by ScaI endonuclease compared with wild type controls, providing further evidence that wild type transcripts are not present in tmem88a mutant embryos ( ). .. Reduced expression of some key cardiac and haematopoietic factors is observed in tmem88a mutant embryos Tmem88a knockdown has previously been shown to reduce expression of haematopoietic genes [ , ].

    Isolation:

    Article Title: Evolutionary Story of a Satellite DNA from Phodopus sungorus (Rodentia, Cricetidae)
    Article Snippet: .. Isolation, Cloning, and Sequencing of PSUcentSat Sequence PSU genomic DNA was digested with the restriction endonuclease (RE) Mbo I, according to the manufacturers’ instructions (Invitrogen Life Technologies), resulting in a smear with DNA fragments ranging between 3 kb and 100 bp. .. The restriction products were later cloned using routine procedures (FERMENTAS Life Science, Invitrogen Life Technologies).

    Incubation:

    Article Title: Multiplex quantitative analysis of microRNA expression via exponential isothermal amplification and conformation-sensitive DNA separation
    Article Snippet: .. The cleavage and control reactions were incubated at 37 °C for 3 h. Bsm DNA polymerase, Buffer R, and Nb.Bpu10I nicking endonuclease were purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA). .. Exponential isothermal amplification Two reaction mixtures were prepared separately on ice.

    Article Title: A novel three-unit tRNA splicing endonuclease found in ultrasmall Archaea possesses broad substrate specificity
    Article Snippet: .. Chemical cross-linking analysis The purified ARMAN-2 tRNA endonuclease (∼500 ng) was incubated in PBS buffer containing 50 mM NaCl with various concentrations (0–5 mM) of BS3 (Bis[sulfosuccinimidyl] suberate) (Thermo Scientific, Rockford, IL, USA) at room temperature for 30 min. .. Reactions were stopped by adding 1 M Tris–HCl (pH 8.0) and analyzed by 10–20% PAGE containing SDS.

    Construct:

    Article Title: HMGB1 released by irradiated tumor cells promotes living tumor cell proliferation via paracrine effect
    Article Snippet: .. In detail, 293 T cells (1 × 106 cells per well; 6-well plate) were transfected with lentiviral construct expressing two gRNAs (gRNA1: 5′ GGAAGAGTCGACTCGCTT 3′, gRNA2: 5′ GTGATGTTGCGAAGAAAC 3′) and Cas9 endonuclease together with ecotropic packaging plasmids, using Lipofectamine 2000 (Invitrogen, Carlsbad, CA) according to manufacturer’s instructions. .. Media containing viruses were collected 48 h after transfection and then utilized to infect HeLa and HT29 cells.

    Purification:

    Article Title: A novel three-unit tRNA splicing endonuclease found in ultrasmall Archaea possesses broad substrate specificity
    Article Snippet: .. Chemical cross-linking analysis The purified ARMAN-2 tRNA endonuclease (∼500 ng) was incubated in PBS buffer containing 50 mM NaCl with various concentrations (0–5 mM) of BS3 (Bis[sulfosuccinimidyl] suberate) (Thermo Scientific, Rockford, IL, USA) at room temperature for 30 min. .. Reactions were stopped by adding 1 M Tris–HCl (pH 8.0) and analyzed by 10–20% PAGE containing SDS.

    Polymerase Chain Reaction:

    Article Title: Diversity of Green-Like and Red-Like Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Large-Subunit Genes (cbbL) in Differently Managed Agricultural Soils
    Article Snippet: .. Ten microliters of each PCR product was hydrolyzed with 2 U of the restriction endonuclease BbvI (MBI Fermentas). .. Restriction fragments were analyzed in 3.5% (wt/vol) agarose gels (PeqLab) and visualized as described previously.

    Generated:

    Article Title: Comparison of Zebrafish tmem88a mutant and morpholino knockdown phenotypes
    Article Snippet: .. In addition, cDNA generated from tmem88a mutants is not digested by ScaI endonuclease compared with wild type controls, providing further evidence that wild type transcripts are not present in tmem88a mutant embryos ( ). .. Reduced expression of some key cardiac and haematopoietic factors is observed in tmem88a mutant embryos Tmem88a knockdown has previously been shown to reduce expression of haematopoietic genes [ , ].

    Activity Assay:

    Article Title: LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity
    Article Snippet: .. In all endonuclease activity assays commercial Eco RI restriction enzyme (Thermo Fisher Scientific) was used as control. .. Reactions were stopped by addition 1/10 volume of stop solution (50 mM EDTA pH 8, 50% glycerol, 0.02% orange G) and products were analyzed by electrophoresis in 1% agarose gels.

    Expressing:

    Article Title: HMGB1 released by irradiated tumor cells promotes living tumor cell proliferation via paracrine effect
    Article Snippet: .. In detail, 293 T cells (1 × 106 cells per well; 6-well plate) were transfected with lentiviral construct expressing two gRNAs (gRNA1: 5′ GGAAGAGTCGACTCGCTT 3′, gRNA2: 5′ GTGATGTTGCGAAGAAAC 3′) and Cas9 endonuclease together with ecotropic packaging plasmids, using Lipofectamine 2000 (Invitrogen, Carlsbad, CA) according to manufacturer’s instructions. .. Media containing viruses were collected 48 h after transfection and then utilized to infect HeLa and HT29 cells.

    Sequencing:

    Article Title: Evolutionary Story of a Satellite DNA from Phodopus sungorus (Rodentia, Cricetidae)
    Article Snippet: .. Isolation, Cloning, and Sequencing of PSUcentSat Sequence PSU genomic DNA was digested with the restriction endonuclease (RE) Mbo I, according to the manufacturers’ instructions (Invitrogen Life Technologies), resulting in a smear with DNA fragments ranging between 3 kb and 100 bp. .. The restriction products were later cloned using routine procedures (FERMENTAS Life Science, Invitrogen Life Technologies).

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  • 99
    Thermo Fisher restriction endonucleases ecori
    Southern blot analysis of the four STS markers- CtR-431, CtR-594., CtR-496, and AFLP-376. Restriction enzymes used are <t>EcoRI,</t> <t>HinDIII</t> and XbaI . M, molecular weight marker; R resistant genotype Punjab Lal; S susceptible genotype Arka Lohit. Hybridization
    Restriction Endonucleases Ecori, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction endonucleases ecori/product/Thermo Fisher
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    restriction endonucleases ecori - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    88
    Thermo Fisher restriction endonucleases eco ri
    Southern blot analysis of the four STS markers- CtR-431, CtR-594., CtR-496, and AFLP-376. Restriction enzymes used are <t>EcoRI,</t> <t>HinDIII</t> and XbaI . M, molecular weight marker; R resistant genotype Punjab Lal; S susceptible genotype Arka Lohit. Hybridization
    Restriction Endonucleases Eco Ri, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction endonucleases eco ri/product/Thermo Fisher
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    restriction endonucleases eco ri - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    99
    Thermo Fisher restriction endonuclease ecorv
    Southern blot analysis of the four STS markers- CtR-431, CtR-594., CtR-496, and AFLP-376. Restriction enzymes used are <t>EcoRI,</t> <t>HinDIII</t> and XbaI . M, molecular weight marker; R resistant genotype Punjab Lal; S susceptible genotype Arka Lohit. Hybridization
    Restriction Endonuclease Ecorv, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction endonuclease ecorv/product/Thermo Fisher
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    restriction endonuclease ecorv - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    Southern blot analysis of the four STS markers- CtR-431, CtR-594., CtR-496, and AFLP-376. Restriction enzymes used are EcoRI, HinDIII and XbaI . M, molecular weight marker; R resistant genotype Punjab Lal; S susceptible genotype Arka Lohit. Hybridization

    Journal: 3 Biotech

    Article Title: Sequence-tagged site-based diagnostic markers linked to a novel anthracnose resistance gene RCt1 in chili pepper (Capsicum annuum L.)

    doi: 10.1007/s13205-018-1552-0

    Figure Lengend Snippet: Southern blot analysis of the four STS markers- CtR-431, CtR-594., CtR-496, and AFLP-376. Restriction enzymes used are EcoRI, HinDIII and XbaI . M, molecular weight marker; R resistant genotype Punjab Lal; S susceptible genotype Arka Lohit. Hybridization

    Article Snippet: Three different restriction endonucleases- EcoRI, HinDIII and XbaI (Thermo Fischer Scientific, USA) were used to digest the genomic DNA from the resistant line ‘Punjab Lal’ and the susceptible line ‘Arka Lohit’.

    Techniques: Southern Blot, Molecular Weight, Marker, Hybridization