restriction endonuclease bstz17 i  (New England Biolabs)


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    Structured Review

    New England Biolabs restriction endonuclease bstz17 i
    A – Non-radioactive PCR-SSCP analysis of VHL exon 3; T – altered mobility observed in a tumor sample, relative to the normal control N. B – Ethidium bromide-stained polyacrylamide gel of <t>BstZ17</t> I restriction digestion. Lane 1 – peripheral venous blood, Lane 2 – renal cell carcinoma, Lane 3 – non tumoral tissue from kidney, Lane 4 – thyroid metastases, M – denotes the lane containing the pUC Mix Marker 8 (Fermentas, Burlingyon, Canada). For the wild allele, restriction digestion produces bands of 212 and 59 bp. The mutation abolishes the restriction site and the mutant allele corresponds to a 271 bp band. C and D – Tumour DNA sequence in the VHL codon 156 region of cloned exon 3 amplicons. As a consequence of the 680 delA, a premature stop codon appears in the mutant allele.
    Restriction Endonuclease Bstz17 I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction endonuclease bstz17 i/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    restriction endonuclease bstz17 i - by Bioz Stars, 2020-04
    86/100 stars

    Images

    1) Product Images from "A multinodular goiter as the initial presentation of a renal cell carcinoma harbouring a novel VHL mutation"

    Article Title: A multinodular goiter as the initial presentation of a renal cell carcinoma harbouring a novel VHL mutation

    Journal: BMC Endocrine Disorders

    doi: 10.1186/1472-6823-6-6

    A – Non-radioactive PCR-SSCP analysis of VHL exon 3; T – altered mobility observed in a tumor sample, relative to the normal control N. B – Ethidium bromide-stained polyacrylamide gel of BstZ17 I restriction digestion. Lane 1 – peripheral venous blood, Lane 2 – renal cell carcinoma, Lane 3 – non tumoral tissue from kidney, Lane 4 – thyroid metastases, M – denotes the lane containing the pUC Mix Marker 8 (Fermentas, Burlingyon, Canada). For the wild allele, restriction digestion produces bands of 212 and 59 bp. The mutation abolishes the restriction site and the mutant allele corresponds to a 271 bp band. C and D – Tumour DNA sequence in the VHL codon 156 region of cloned exon 3 amplicons. As a consequence of the 680 delA, a premature stop codon appears in the mutant allele.
    Figure Legend Snippet: A – Non-radioactive PCR-SSCP analysis of VHL exon 3; T – altered mobility observed in a tumor sample, relative to the normal control N. B – Ethidium bromide-stained polyacrylamide gel of BstZ17 I restriction digestion. Lane 1 – peripheral venous blood, Lane 2 – renal cell carcinoma, Lane 3 – non tumoral tissue from kidney, Lane 4 – thyroid metastases, M – denotes the lane containing the pUC Mix Marker 8 (Fermentas, Burlingyon, Canada). For the wild allele, restriction digestion produces bands of 212 and 59 bp. The mutation abolishes the restriction site and the mutant allele corresponds to a 271 bp band. C and D – Tumour DNA sequence in the VHL codon 156 region of cloned exon 3 amplicons. As a consequence of the 680 delA, a premature stop codon appears in the mutant allele.

    Techniques Used: Polymerase Chain Reaction, Staining, Marker, Mutagenesis, Sequencing, Clone Assay

    Related Articles

    Mutagenesis:

    Article Title: A multinodular goiter as the initial presentation of a renal cell carcinoma harbouring a novel VHL mutation
    Article Snippet: Paragraph title: VHL mutation analysis ... Restriction analysis, using the restriction endonuclease BstZ17 I (New England BioLabs® , Inc., Beverly, USA) was also performed.

    Isolation:

    Article Title: A multinodular goiter as the initial presentation of a renal cell carcinoma harbouring a novel VHL mutation
    Article Snippet: Genomic DNA was also obtained from peripheral venous blood and isolated by a manual method adapted from Bowtell [ ]. .. Restriction analysis, using the restriction endonuclease BstZ17 I (New England BioLabs® , Inc., Beverly, USA) was also performed.

    Purification:

    Article Title: A multinodular goiter as the initial presentation of a renal cell carcinoma harbouring a novel VHL mutation
    Article Snippet: To further characterize the abnormal pattern observed in the SSCP, PCR purified products were either sequenced directly using the ABI PRISM® BigDye™ Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems, Foster City, CA, USA) and the ABI PRISM 310 Genetic Analyser or subcloned into pGEM® -T Easy Vector (Promega, Madison, USA), and subsequently sequenced using the ABI PRISM® BigDye™ Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems, Foster City, CA, USA) and the ABI PRISM 310 Genetic Analyser. .. Restriction analysis, using the restriction endonuclease BstZ17 I (New England BioLabs® , Inc., Beverly, USA) was also performed.

    Polymerase Chain Reaction:

    Article Title: A multinodular goiter as the initial presentation of a renal cell carcinoma harbouring a novel VHL mutation
    Article Snippet: To further characterize the abnormal pattern observed in the SSCP, PCR purified products were either sequenced directly using the ABI PRISM® BigDye™ Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems, Foster City, CA, USA) and the ABI PRISM 310 Genetic Analyser or subcloned into pGEM® -T Easy Vector (Promega, Madison, USA), and subsequently sequenced using the ABI PRISM® BigDye™ Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems, Foster City, CA, USA) and the ABI PRISM 310 Genetic Analyser. .. Restriction analysis, using the restriction endonuclease BstZ17 I (New England BioLabs® , Inc., Beverly, USA) was also performed.

    Sequencing:

    Article Title: A multinodular goiter as the initial presentation of a renal cell carcinoma harbouring a novel VHL mutation
    Article Snippet: To further characterize the abnormal pattern observed in the SSCP, PCR purified products were either sequenced directly using the ABI PRISM® BigDye™ Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems, Foster City, CA, USA) and the ABI PRISM 310 Genetic Analyser or subcloned into pGEM® -T Easy Vector (Promega, Madison, USA), and subsequently sequenced using the ABI PRISM® BigDye™ Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems, Foster City, CA, USA) and the ABI PRISM 310 Genetic Analyser. .. Restriction analysis, using the restriction endonuclease BstZ17 I (New England BioLabs® , Inc., Beverly, USA) was also performed.

    Plasmid Preparation:

    Article Title: A multinodular goiter as the initial presentation of a renal cell carcinoma harbouring a novel VHL mutation
    Article Snippet: To further characterize the abnormal pattern observed in the SSCP, PCR purified products were either sequenced directly using the ABI PRISM® BigDye™ Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems, Foster City, CA, USA) and the ABI PRISM 310 Genetic Analyser or subcloned into pGEM® -T Easy Vector (Promega, Madison, USA), and subsequently sequenced using the ABI PRISM® BigDye™ Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems, Foster City, CA, USA) and the ABI PRISM 310 Genetic Analyser. .. Restriction analysis, using the restriction endonuclease BstZ17 I (New England BioLabs® , Inc., Beverly, USA) was also performed.

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  • 86
    New England Biolabs restriction endonuclease bstz17 i
    A – Non-radioactive PCR-SSCP analysis of VHL exon 3; T – altered mobility observed in a tumor sample, relative to the normal control N. B – Ethidium bromide-stained polyacrylamide gel of <t>BstZ17</t> I restriction digestion. Lane 1 – peripheral venous blood, Lane 2 – renal cell carcinoma, Lane 3 – non tumoral tissue from kidney, Lane 4 – thyroid metastases, M – denotes the lane containing the pUC Mix Marker 8 (Fermentas, Burlingyon, Canada). For the wild allele, restriction digestion produces bands of 212 and 59 bp. The mutation abolishes the restriction site and the mutant allele corresponds to a 271 bp band. C and D – Tumour DNA sequence in the VHL codon 156 region of cloned exon 3 amplicons. As a consequence of the 680 delA, a premature stop codon appears in the mutant allele.
    Restriction Endonuclease Bstz17 I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction endonuclease bstz17 i/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    restriction endonuclease bstz17 i - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

    Image Search Results


    A – Non-radioactive PCR-SSCP analysis of VHL exon 3; T – altered mobility observed in a tumor sample, relative to the normal control N. B – Ethidium bromide-stained polyacrylamide gel of BstZ17 I restriction digestion. Lane 1 – peripheral venous blood, Lane 2 – renal cell carcinoma, Lane 3 – non tumoral tissue from kidney, Lane 4 – thyroid metastases, M – denotes the lane containing the pUC Mix Marker 8 (Fermentas, Burlingyon, Canada). For the wild allele, restriction digestion produces bands of 212 and 59 bp. The mutation abolishes the restriction site and the mutant allele corresponds to a 271 bp band. C and D – Tumour DNA sequence in the VHL codon 156 region of cloned exon 3 amplicons. As a consequence of the 680 delA, a premature stop codon appears in the mutant allele.

    Journal: BMC Endocrine Disorders

    Article Title: A multinodular goiter as the initial presentation of a renal cell carcinoma harbouring a novel VHL mutation

    doi: 10.1186/1472-6823-6-6

    Figure Lengend Snippet: A – Non-radioactive PCR-SSCP analysis of VHL exon 3; T – altered mobility observed in a tumor sample, relative to the normal control N. B – Ethidium bromide-stained polyacrylamide gel of BstZ17 I restriction digestion. Lane 1 – peripheral venous blood, Lane 2 – renal cell carcinoma, Lane 3 – non tumoral tissue from kidney, Lane 4 – thyroid metastases, M – denotes the lane containing the pUC Mix Marker 8 (Fermentas, Burlingyon, Canada). For the wild allele, restriction digestion produces bands of 212 and 59 bp. The mutation abolishes the restriction site and the mutant allele corresponds to a 271 bp band. C and D – Tumour DNA sequence in the VHL codon 156 region of cloned exon 3 amplicons. As a consequence of the 680 delA, a premature stop codon appears in the mutant allele.

    Article Snippet: Restriction analysis, using the restriction endonuclease BstZ17 I (New England BioLabs® , Inc., Beverly, USA) was also performed.

    Techniques: Polymerase Chain Reaction, Staining, Marker, Mutagenesis, Sequencing, Clone Assay