Structured Review

TaKaRa red fluorescent protein dsred2
Electropherogram of individual mitochondria-cytoskeleton binding events. (A) Top and bottom traces show A488-PHD and <t>DsRed2</t> detection, respectively. (B) Migration time window 538–546 s from (A) showing representative individually detected events.
Red Fluorescent Protein Dsred2, supplied by TaKaRa, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/red fluorescent protein dsred2/product/TaKaRa
Average 88 stars, based on 7 article reviews
Price from $9.99 to $1999.99
red fluorescent protein dsred2 - by Bioz Stars, 2021-01
88/100 stars

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1) Product Images from "Capillary electrophoretic analysis reveals subcellular binding between individual mitochondria and cytoskeleton"

Article Title: Capillary electrophoretic analysis reveals subcellular binding between individual mitochondria and cytoskeleton

Journal: Analytical chemistry

doi: 10.1021/ac200068p

Electropherogram of individual mitochondria-cytoskeleton binding events. (A) Top and bottom traces show A488-PHD and DsRed2 detection, respectively. (B) Migration time window 538–546 s from (A) showing representative individually detected events.
Figure Legend Snippet: Electropherogram of individual mitochondria-cytoskeleton binding events. (A) Top and bottom traces show A488-PHD and DsRed2 detection, respectively. (B) Migration time window 538–546 s from (A) showing representative individually detected events.

Techniques Used: Binding Assay, Migration

Fluorescence imaging of mitochondria and cytoskeleton. ( A ) F-actin cytoskeleton labeled with A488-PHD. ( B ) Mitochondria labeled with DsRed2. ( C ) Overlay of (A) and (B); the nucleus is counterstained with DAPI. The images are deconvoluted Z-stacks of confocal
Figure Legend Snippet: Fluorescence imaging of mitochondria and cytoskeleton. ( A ) F-actin cytoskeleton labeled with A488-PHD. ( B ) Mitochondria labeled with DsRed2. ( C ) Overlay of (A) and (B); the nucleus is counterstained with DAPI. The images are deconvoluted Z-stacks of confocal

Techniques Used: Fluorescence, Imaging, Labeling

Related Articles

Polymerase Chain Reaction:

Article Title: Migratory Dermal Dendritic Cells Act as Rapid Sensors of Protozoan Parasites
Article Snippet: .. To generate fluorescent protein expressing L. major parasites, the gene encoding the red fluorescent protein DsRed2 was PCR amplified from pDsRed2 (Clontech) with primers that added BamHI sites to both ends. .. The PCR product was cut with BamHI and ligated into BglII site of pIR1SAT yielding pIR1SAT-DsRed2 (strain B4787).

Transfection:

Article Title: Capillary electrophoretic analysis reveals subcellular binding between individual mitochondria and cytoskeleton
Article Snippet: .. Cells were transfected with a plasmid harboring genes responsible for neomycin/kanamycin resistance and expression of mito-DsRed2, a fusion protein that targets mitochondria to release the red fluorescent protein DsRed2 into the mitochondrial matrix (Clonetech, Mountain View, CA). .. Transfections were performed using the lipofection reagent DMRIE-C according to the manufacturer’s instructions.

Amplification:

Article Title: Migratory Dermal Dendritic Cells Act as Rapid Sensors of Protozoan Parasites
Article Snippet: .. To generate fluorescent protein expressing L. major parasites, the gene encoding the red fluorescent protein DsRed2 was PCR amplified from pDsRed2 (Clontech) with primers that added BamHI sites to both ends. .. The PCR product was cut with BamHI and ligated into BglII site of pIR1SAT yielding pIR1SAT-DsRed2 (strain B4787).

Plasmid Preparation:

Article Title: Capillary electrophoretic analysis reveals subcellular binding between individual mitochondria and cytoskeleton
Article Snippet: .. Cells were transfected with a plasmid harboring genes responsible for neomycin/kanamycin resistance and expression of mito-DsRed2, a fusion protein that targets mitochondria to release the red fluorescent protein DsRed2 into the mitochondrial matrix (Clonetech, Mountain View, CA). .. Transfections were performed using the lipofection reagent DMRIE-C according to the manufacturer’s instructions.

Article Title: A novel plasmid for delivering genes into mammalian cells with noninvasive food and commensal lactic acid bacteria
Article Snippet: .. A promoterless 0.7-kb 'rfp (' rfp ) cassette encoding the red fluorescent protein DsRed2 was obtained from the plasmid pDsRed2 (Clontech) by digestion with restriction enzymes Bam HI and Eco RI. .. It was subcloned into pLKV1 multiple cloning site (MCS) downstream of the CMV promoter resulting in pLKV-Red2 (5.1 kb).

Expressing:

Article Title: Capillary electrophoretic analysis reveals subcellular binding between individual mitochondria and cytoskeleton
Article Snippet: .. Cells were transfected with a plasmid harboring genes responsible for neomycin/kanamycin resistance and expression of mito-DsRed2, a fusion protein that targets mitochondria to release the red fluorescent protein DsRed2 into the mitochondrial matrix (Clonetech, Mountain View, CA). .. Transfections were performed using the lipofection reagent DMRIE-C according to the manufacturer’s instructions.

Article Title: Migratory Dermal Dendritic Cells Act as Rapid Sensors of Protozoan Parasites
Article Snippet: .. To generate fluorescent protein expressing L. major parasites, the gene encoding the red fluorescent protein DsRed2 was PCR amplified from pDsRed2 (Clontech) with primers that added BamHI sites to both ends. .. The PCR product was cut with BamHI and ligated into BglII site of pIR1SAT yielding pIR1SAT-DsRed2 (strain B4787).

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    TaKaRa red fluorescent protein dsred2
    Electropherogram of individual mitochondria-cytoskeleton binding events. (A) Top and bottom traces show A488-PHD and <t>DsRed2</t> detection, respectively. (B) Migration time window 538–546 s from (A) showing representative individually detected events.
    Red Fluorescent Protein Dsred2, supplied by TaKaRa, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/red fluorescent protein dsred2/product/TaKaRa
    Average 88 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    red fluorescent protein dsred2 - by Bioz Stars, 2021-01
    88/100 stars
      Buy from Supplier

    85
    TaKaRa red fluorescent protein dsred2 gene
    Schematic drawing of lentiviral vectors used in this study. LTR, lentiviral long terminal repeat; Tretight, a modified tetracycline-responsive promoter derived from <t>pTRE-tight-DsRed2</t> (Clontech); DsRed2, red fluorescent protein; 5 × GAL4BS, five tandem GAL4 binding sites; SYN, human synapsin 1 promoter; GfaABC 1 D, a compact glial fibrillary acidic protein promoter; GAL4p65, a chimeric transactivator consisting of a part of the transactivatin domain of the murine NF-κB p65 protein fused to the DNA-binding domain of the GAL4 protein from yeast; tTA, tetracycline-controlled transactivator protein
    Red Fluorescent Protein Dsred2 Gene, supplied by TaKaRa, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/red fluorescent protein dsred2 gene/product/TaKaRa
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    red fluorescent protein dsred2 gene - by Bioz Stars, 2021-01
    85/100 stars
      Buy from Supplier

    92
    TaKaRa red fluorescent protein
    Schematic drawing of lentiviral vectors used in this study. LTR, lentiviral long terminal repeat; Tretight, a modified tetracycline-responsive promoter derived from <t>pTRE-tight-DsRed2</t> (Clontech); DsRed2, red fluorescent protein; 5 × GAL4BS, five tandem GAL4 binding sites; SYN, human synapsin 1 promoter; GfaABC 1 D, a compact glial fibrillary acidic protein promoter; GAL4p65, a chimeric transactivator consisting of a part of the transactivatin domain of the murine NF-κB p65 protein fused to the DNA-binding domain of the GAL4 protein from yeast; tTA, tetracycline-controlled transactivator protein
    Red Fluorescent Protein, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/red fluorescent protein/product/TaKaRa
    Average 92 stars, based on 57 article reviews
    Price from $9.99 to $1999.99
    red fluorescent protein - by Bioz Stars, 2021-01
    92/100 stars
      Buy from Supplier

    N/A
    DsRed2 retains the benefits typical of red fluorescent proteins such as a high signal to noise ratio and distinct spectral properties for use in multicolor labeling experiments DsRed2 is a
      Buy from Supplier

    Image Search Results


    Electropherogram of individual mitochondria-cytoskeleton binding events. (A) Top and bottom traces show A488-PHD and DsRed2 detection, respectively. (B) Migration time window 538–546 s from (A) showing representative individually detected events.

    Journal: Analytical chemistry

    Article Title: Capillary electrophoretic analysis reveals subcellular binding between individual mitochondria and cytoskeleton

    doi: 10.1021/ac200068p

    Figure Lengend Snippet: Electropherogram of individual mitochondria-cytoskeleton binding events. (A) Top and bottom traces show A488-PHD and DsRed2 detection, respectively. (B) Migration time window 538–546 s from (A) showing representative individually detected events.

    Article Snippet: Cells were transfected with a plasmid harboring genes responsible for neomycin/kanamycin resistance and expression of mito-DsRed2, a fusion protein that targets mitochondria to release the red fluorescent protein DsRed2 into the mitochondrial matrix (Clonetech, Mountain View, CA).

    Techniques: Binding Assay, Migration

    Fluorescence imaging of mitochondria and cytoskeleton. ( A ) F-actin cytoskeleton labeled with A488-PHD. ( B ) Mitochondria labeled with DsRed2. ( C ) Overlay of (A) and (B); the nucleus is counterstained with DAPI. The images are deconvoluted Z-stacks of confocal

    Journal: Analytical chemistry

    Article Title: Capillary electrophoretic analysis reveals subcellular binding between individual mitochondria and cytoskeleton

    doi: 10.1021/ac200068p

    Figure Lengend Snippet: Fluorescence imaging of mitochondria and cytoskeleton. ( A ) F-actin cytoskeleton labeled with A488-PHD. ( B ) Mitochondria labeled with DsRed2. ( C ) Overlay of (A) and (B); the nucleus is counterstained with DAPI. The images are deconvoluted Z-stacks of confocal

    Article Snippet: Cells were transfected with a plasmid harboring genes responsible for neomycin/kanamycin resistance and expression of mito-DsRed2, a fusion protein that targets mitochondria to release the red fluorescent protein DsRed2 into the mitochondrial matrix (Clonetech, Mountain View, CA).

    Techniques: Fluorescence, Imaging, Labeling

    Transient expression of red fluorescent protein DsRed2 by BME26 cells transfected with a cis- plasmid containing the Sleeping Beauty SB10 transposase and a transposon containing the DsRed2 gene. Data points are the means±standard deviations. Inset:

    Journal: Insect biochemistry and molecular biology

    Article Title: Cellular and molecular characterization of an embryonic cell line (BME26) from the tick Rhipicephalus (Boophilus) microplus

    doi: 10.1016/j.ibmb.2008.01.006

    Figure Lengend Snippet: Transient expression of red fluorescent protein DsRed2 by BME26 cells transfected with a cis- plasmid containing the Sleeping Beauty SB10 transposase and a transposon containing the DsRed2 gene. Data points are the means±standard deviations. Inset:

    Article Snippet: In this construct the SB 10 transposase gene, driven by the cytomegalovirus (CMV) promoter, resided outside the IR/DR of the transposable element containing the reporter gene, red fluorescent protein DsRed2 (Clontech).

    Techniques: Expressing, Transfection, Plasmid Preparation

    Schematic drawing of lentiviral vectors used in this study. LTR, lentiviral long terminal repeat; Tretight, a modified tetracycline-responsive promoter derived from pTRE-tight-DsRed2 (Clontech); DsRed2, red fluorescent protein; 5 × GAL4BS, five tandem GAL4 binding sites; SYN, human synapsin 1 promoter; GfaABC 1 D, a compact glial fibrillary acidic protein promoter; GAL4p65, a chimeric transactivator consisting of a part of the transactivatin domain of the murine NF-κB p65 protein fused to the DNA-binding domain of the GAL4 protein from yeast; tTA, tetracycline-controlled transactivator protein

    Journal: The Journal of Gene Medicine

    Article Title: Enhancement of cell-specific transgene expression from a Tet-Off regulatory system using a transcriptional amplification strategy in the rat brain

    doi: 10.1002/jgm.1178

    Figure Lengend Snippet: Schematic drawing of lentiviral vectors used in this study. LTR, lentiviral long terminal repeat; Tretight, a modified tetracycline-responsive promoter derived from pTRE-tight-DsRed2 (Clontech); DsRed2, red fluorescent protein; 5 × GAL4BS, five tandem GAL4 binding sites; SYN, human synapsin 1 promoter; GfaABC 1 D, a compact glial fibrillary acidic protein promoter; GAL4p65, a chimeric transactivator consisting of a part of the transactivatin domain of the murine NF-κB p65 protein fused to the DNA-binding domain of the GAL4 protein from yeast; tTA, tetracycline-controlled transactivator protein

    Article Snippet: To generate the LV-Tretight-DsRed2 shuttle vector, the Tretight-DsRed2 fragment containing the modified Tet-responsive promoter and the red fluorescent protein (DsRed2) gene was excised from pTRE-Tight-DsRed2 (Clontech) with Xho I/Not I and cloned into pTYF-SW-Linker.

    Techniques: Modification, Derivative Assay, Binding Assay

    Quantification of in vivo transgene expression from TA-enhanced Tet-Off regulatory systems. Viruses were injected into the rat brain as described, see legend for Figure 4 . (A) SYN-based system. (B) GfaABC 1 D-based system. Seven days after injection, numbers of DsRed2-positive cells per view of field were counted under 400× magnification. For each rat, four 40 µm coronal sections surrounding the injection tract and three fields in each section were selected randomly for cell counting (n = 3 rats per group). *, ** p

    Journal: The Journal of Gene Medicine

    Article Title: Enhancement of cell-specific transgene expression from a Tet-Off regulatory system using a transcriptional amplification strategy in the rat brain

    doi: 10.1002/jgm.1178

    Figure Lengend Snippet: Quantification of in vivo transgene expression from TA-enhanced Tet-Off regulatory systems. Viruses were injected into the rat brain as described, see legend for Figure 4 . (A) SYN-based system. (B) GfaABC 1 D-based system. Seven days after injection, numbers of DsRed2-positive cells per view of field were counted under 400× magnification. For each rat, four 40 µm coronal sections surrounding the injection tract and three fields in each section were selected randomly for cell counting (n = 3 rats per group). *, ** p

    Article Snippet: To generate the LV-Tretight-DsRed2 shuttle vector, the Tretight-DsRed2 fragment containing the modified Tet-responsive promoter and the red fluorescent protein (DsRed2) gene was excised from pTRE-Tight-DsRed2 (Clontech) with Xho I/Not I and cloned into pTYF-SW-Linker.

    Techniques: In Vivo, Expressing, Injection, Cell Counting

    DsRed2 transgene expression in the hypoglossal motor nucleus of the rat brain. LV-Tretight-DsRed2 was injected with either LV-1 × SYN-tTA (a), LV-2 × SYN-tTA (b, c, d, e), LV-1 × GfaABC1D-tTA (f), or LV-2 × GfaABC1D-tTA (g, h, i, j). The ratio between the transactivator-expressing and Tre viruses was 4 : 1 and a total of 6 × 10 6 IU of viruses was injected per rat. (a, b, f, g) Seven days after injection and without Dox treatment. DsRed2 expression was observed in all four groups. (c, h) Virus-injected rats drank Dox-containing water for 7 days. DsRed2 expression was completely repressed. (d, i) Dox treatment for the first 7 days after injection followed by Dox-free water for another 7 days. Repressed DsRed2 gene was retrieved maximally. (e, j) Dox-free water for the first 7 days after injection followed by Dox-containing water for another 7 days. Note DsRed2 expression was almost completely abolished

    Journal: The Journal of Gene Medicine

    Article Title: Enhancement of cell-specific transgene expression from a Tet-Off regulatory system using a transcriptional amplification strategy in the rat brain

    doi: 10.1002/jgm.1178

    Figure Lengend Snippet: DsRed2 transgene expression in the hypoglossal motor nucleus of the rat brain. LV-Tretight-DsRed2 was injected with either LV-1 × SYN-tTA (a), LV-2 × SYN-tTA (b, c, d, e), LV-1 × GfaABC1D-tTA (f), or LV-2 × GfaABC1D-tTA (g, h, i, j). The ratio between the transactivator-expressing and Tre viruses was 4 : 1 and a total of 6 × 10 6 IU of viruses was injected per rat. (a, b, f, g) Seven days after injection and without Dox treatment. DsRed2 expression was observed in all four groups. (c, h) Virus-injected rats drank Dox-containing water for 7 days. DsRed2 expression was completely repressed. (d, i) Dox treatment for the first 7 days after injection followed by Dox-free water for another 7 days. Repressed DsRed2 gene was retrieved maximally. (e, j) Dox-free water for the first 7 days after injection followed by Dox-containing water for another 7 days. Note DsRed2 expression was almost completely abolished

    Article Snippet: To generate the LV-Tretight-DsRed2 shuttle vector, the Tretight-DsRed2 fragment containing the modified Tet-responsive promoter and the red fluorescent protein (DsRed2) gene was excised from pTRE-Tight-DsRed2 (Clontech) with Xho I/Not I and cloned into pTYF-SW-Linker.

    Techniques: Expressing, Injection

    Quantification of in vitro transgene expression from transcriptional amplification-enhanced Tet-Off regulatory systems at MOIs of 1, 5, 15 and 25. (A) SYN-containing system in neuronal PC12 cells. (B) GfaABC1D-containing system in astroglial 1321N1 cells. Numbers of DsRed2-positive cells per field of view were counted under 100× magnification. Six fields were selected randomly for cell counting. The error bars indicate the standard deviations

    Journal: The Journal of Gene Medicine

    Article Title: Enhancement of cell-specific transgene expression from a Tet-Off regulatory system using a transcriptional amplification strategy in the rat brain

    doi: 10.1002/jgm.1178

    Figure Lengend Snippet: Quantification of in vitro transgene expression from transcriptional amplification-enhanced Tet-Off regulatory systems at MOIs of 1, 5, 15 and 25. (A) SYN-containing system in neuronal PC12 cells. (B) GfaABC1D-containing system in astroglial 1321N1 cells. Numbers of DsRed2-positive cells per field of view were counted under 100× magnification. Six fields were selected randomly for cell counting. The error bars indicate the standard deviations

    Article Snippet: To generate the LV-Tretight-DsRed2 shuttle vector, the Tretight-DsRed2 fragment containing the modified Tet-responsive promoter and the red fluorescent protein (DsRed2) gene was excised from pTRE-Tight-DsRed2 (Clontech) with Xho I/Not I and cloned into pTYF-SW-Linker.

    Techniques: In Vitro, Expressing, Amplification, Cell Counting

    DsRed2 transgene expression in vitro in neuronal PC12 (a–e) and astroglial 1321N1 (f–j) cells. Cells were transduced with LV-Tretight-DsRed2 and either LV-1 × SYN-tTA (a), LV-2 × SYN-tTA (b, c, d, e), LV-1 × GfaABC1D-tTA (f), or LV-2 × GfaABC1D-tTA (g, h, i, j). The ratio between the trasactivator and Tre viruses was 1 : 1, with a total viral MOI of 5 per well. (a, b, f, g) Forty-eight hours after transduction without Dox treatment. DsRed2 expression was observed in all four groups. (c, h) Forty-eight hours after transduction in the presence of Dox. DsRed2 expression was completely repressed. (d, i) Dox was present for 48 h after transduction followed by a change to Dox-free medium and culturing for 4 more days. Repressed DsRed2 gene was retrieved maximally. (e, j) Dox was absent for 48 h after transduction followed by a change to Dox-containing medium and culturing for 4 more days. Note DsRed2 expression was completely abolished

    Journal: The Journal of Gene Medicine

    Article Title: Enhancement of cell-specific transgene expression from a Tet-Off regulatory system using a transcriptional amplification strategy in the rat brain

    doi: 10.1002/jgm.1178

    Figure Lengend Snippet: DsRed2 transgene expression in vitro in neuronal PC12 (a–e) and astroglial 1321N1 (f–j) cells. Cells were transduced with LV-Tretight-DsRed2 and either LV-1 × SYN-tTA (a), LV-2 × SYN-tTA (b, c, d, e), LV-1 × GfaABC1D-tTA (f), or LV-2 × GfaABC1D-tTA (g, h, i, j). The ratio between the trasactivator and Tre viruses was 1 : 1, with a total viral MOI of 5 per well. (a, b, f, g) Forty-eight hours after transduction without Dox treatment. DsRed2 expression was observed in all four groups. (c, h) Forty-eight hours after transduction in the presence of Dox. DsRed2 expression was completely repressed. (d, i) Dox was present for 48 h after transduction followed by a change to Dox-free medium and culturing for 4 more days. Repressed DsRed2 gene was retrieved maximally. (e, j) Dox was absent for 48 h after transduction followed by a change to Dox-containing medium and culturing for 4 more days. Note DsRed2 expression was completely abolished

    Article Snippet: To generate the LV-Tretight-DsRed2 shuttle vector, the Tretight-DsRed2 fragment containing the modified Tet-responsive promoter and the red fluorescent protein (DsRed2) gene was excised from pTRE-Tight-DsRed2 (Clontech) with Xho I/Not I and cloned into pTYF-SW-Linker.

    Techniques: Expressing, In Vitro, Transduction