recombinant sars cov 2 nucleocapsid phosphoprotein  (Native Antigen Inc)

 
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    Name:
    SARS CoV 2 Nucleoprotein His tag E coli
    Description:
    Recombinant SARS CoV 2 nucleocapsid phosphoprotein GenBank MN908947 isolate Wuhan Hu 1 expressed and purified from E coli as full length nucleoprotein amino acids 1 419 with a C terminal His tag
    Catalog Number:
    REC31812-100
    Price:
    None
    Category:
    Recombinant Antigen
    Source:
    E. coli recombinant
    Purity:
    95% purity (10% SDS-PAGE, coomassie blue staining).
    Size:
    100µg
    Format:
    Liquid
    Buy from Supplier


    Structured Review

    Native Antigen Inc recombinant sars cov 2 nucleocapsid phosphoprotein
    SARS CoV 2 Nucleoprotein His tag E coli
    Recombinant SARS CoV 2 nucleocapsid phosphoprotein GenBank MN908947 isolate Wuhan Hu 1 expressed and purified from E coli as full length nucleoprotein amino acids 1 419 with a C terminal His tag
    https://www.bioz.com/result/recombinant sars cov 2 nucleocapsid phosphoprotein/product/Native Antigen Inc
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant sars cov 2 nucleocapsid phosphoprotein - by Bioz Stars, 2021-09
    95/100 stars

    Images

    1) Product Images from "Comparison of Rhesus and Cynomolgus macaques as an authentic model for COVID-19"

    Article Title: Comparison of Rhesus and Cynomolgus macaques as an authentic model for COVID-19

    Journal: bioRxiv

    doi: 10.1101/2020.09.17.301093

    Cellular immune responses to SARS-CoV-2. A, B) IFNγ SFU measured in PBMCs and stimulated with spike protein peptide pools (PP) peptide in A) rhesus and B) cynomolgus macaques. PBMC samples were isolated from uninfected animals (naïve) or at early (days 4 and 5) and late (days 14-19) time-points following SARS-CoV-2 infection. Box plots show the group median +/- inter-quartile range, with minimum and maximum values connected by whiskers. C, D) IFNγ SFU measured in PBMC in response to spike protein megapools (MP) in C) rhesus and D) cynomolgus macaques or, E) in mononuclear cells isolated from lung and spleen. Bars show the group median with SFU measured in individual animals shown as dots. * p ≤ 0.05 , ** p ≤ 0.01. F-J) Frequency of major lymphocyte and monocyte cell populations quantified by immunophenotyping assay F - H) CD4+, CD8+ and γδ T-cell frequencies in PBMCs and lung cells, I) Monocyte subtype frequency in PBMCs and lung MNCs, J) Natural killer (NK) cell subset frequency in PBMCs and lung MNCs. Stacked bars show the group median with 95% confidence intervals. PBMC: Naïve rhesus n=8, early rhesus n= 1, late rhesus n=2, naïve cyno = 7, early cyno n=2, late cyno n=2. Lung: early rhesus n= 2, late rhesus n=3, early cyno n=2, late cyno n=2. K-N) Intracellular cytokine staining data K-L) Cytokine and activation marker detection in CD4+, CD8+ and γδ T-cells in PBMCs stimulated with M, N and S peptide pools. G-N) CD107a expression in CD8+ and γδ T-cells in PBMCs. Bars show the group median with cell frequencies measured in individual animals shown as dots.
    Figure Legend Snippet: Cellular immune responses to SARS-CoV-2. A, B) IFNγ SFU measured in PBMCs and stimulated with spike protein peptide pools (PP) peptide in A) rhesus and B) cynomolgus macaques. PBMC samples were isolated from uninfected animals (naïve) or at early (days 4 and 5) and late (days 14-19) time-points following SARS-CoV-2 infection. Box plots show the group median +/- inter-quartile range, with minimum and maximum values connected by whiskers. C, D) IFNγ SFU measured in PBMC in response to spike protein megapools (MP) in C) rhesus and D) cynomolgus macaques or, E) in mononuclear cells isolated from lung and spleen. Bars show the group median with SFU measured in individual animals shown as dots. * p ≤ 0.05 , ** p ≤ 0.01. F-J) Frequency of major lymphocyte and monocyte cell populations quantified by immunophenotyping assay F - H) CD4+, CD8+ and γδ T-cell frequencies in PBMCs and lung cells, I) Monocyte subtype frequency in PBMCs and lung MNCs, J) Natural killer (NK) cell subset frequency in PBMCs and lung MNCs. Stacked bars show the group median with 95% confidence intervals. PBMC: Naïve rhesus n=8, early rhesus n= 1, late rhesus n=2, naïve cyno = 7, early cyno n=2, late cyno n=2. Lung: early rhesus n= 2, late rhesus n=3, early cyno n=2, late cyno n=2. K-N) Intracellular cytokine staining data K-L) Cytokine and activation marker detection in CD4+, CD8+ and γδ T-cells in PBMCs stimulated with M, N and S peptide pools. G-N) CD107a expression in CD8+ and γδ T-cells in PBMCs. Bars show the group median with cell frequencies measured in individual animals shown as dots.

    Techniques Used: Isolation, Infection, Staining, Activation Assay, Marker, Expressing

    Histopathological changes in cynomolgus and rhesus macaques during SARS-CoV-2 infection. Diffuse areas of DAD observed in cynomolgus macaques at 4/5 dpc with shrunken, eosinophilic cells within the alveolar walls (A, B), together with alveolar oedema (A, arrows) pneumocyte hyperplasia and expanded alveolar spaces with inflammatory cell infiltration (B, arrows). Occasional multinucleated cells resembling syncytial cells are observed (B, insert). ISH detection of viral RNA (RNAScope, red chromogen) within the areas of pneumonia (C) and occasionally in the BALT (C, insert). Abundant IL-6 producing cells observed in the areas of pneumonia (D) Similar histopathological changes observed in rhesus macaques, including DAD areas with patchy alveolar oedema (E, arrow), alveolar macrophage hyperplasia (F, arrow), bronchial exudates and presence of viral RNA within the areas showing pneumonia (G) and abundant IL-6 producing cells (H). Histopathological changes with less severity observed at 14/15 dpc in cynomolgus macaques, with infiltration of mononuclear cells within alveolar spaces and bronchiolar luminae (I, arrows) and parenchymal collapse (I, *) and perivascular cuffing (J, arrow), with minimal detection of viral RNA in pneumocytes (J, insert). Bronchiole regeneration (K, arrow) and perivascular/peribronchiolar cuffing observed in rhesus macaques at 14/15 dpc (L, arrows), together with BALT proliferation (L, *) with minimal presence of viral RNA (L, insert).
    Figure Legend Snippet: Histopathological changes in cynomolgus and rhesus macaques during SARS-CoV-2 infection. Diffuse areas of DAD observed in cynomolgus macaques at 4/5 dpc with shrunken, eosinophilic cells within the alveolar walls (A, B), together with alveolar oedema (A, arrows) pneumocyte hyperplasia and expanded alveolar spaces with inflammatory cell infiltration (B, arrows). Occasional multinucleated cells resembling syncytial cells are observed (B, insert). ISH detection of viral RNA (RNAScope, red chromogen) within the areas of pneumonia (C) and occasionally in the BALT (C, insert). Abundant IL-6 producing cells observed in the areas of pneumonia (D) Similar histopathological changes observed in rhesus macaques, including DAD areas with patchy alveolar oedema (E, arrow), alveolar macrophage hyperplasia (F, arrow), bronchial exudates and presence of viral RNA within the areas showing pneumonia (G) and abundant IL-6 producing cells (H). Histopathological changes with less severity observed at 14/15 dpc in cynomolgus macaques, with infiltration of mononuclear cells within alveolar spaces and bronchiolar luminae (I, arrows) and parenchymal collapse (I, *) and perivascular cuffing (J, arrow), with minimal detection of viral RNA in pneumocytes (J, insert). Bronchiole regeneration (K, arrow) and perivascular/peribronchiolar cuffing observed in rhesus macaques at 14/15 dpc (L, arrows), together with BALT proliferation (L, *) with minimal presence of viral RNA (L, insert).

    Techniques Used: Infection, In Situ Hybridization

    Lung histopathology scores and presence of viral RNA and IL-6 by ISH in cynomolgus and rhesus macaques during SARS-CoV-2 infection. Heatmap showing the individual and aggregate (TOTAL) scores for each lung histopathological parameter and animal (A). Image analysis of positively stained area in RNASCope labelled sections for viral RNA (B; whole slide) and IL-6 mRNA (C, areas of lesion), showing data for individual animals with mean value for each group (box).
    Figure Legend Snippet: Lung histopathology scores and presence of viral RNA and IL-6 by ISH in cynomolgus and rhesus macaques during SARS-CoV-2 infection. Heatmap showing the individual and aggregate (TOTAL) scores for each lung histopathological parameter and animal (A). Image analysis of positively stained area in RNASCope labelled sections for viral RNA (B; whole slide) and IL-6 mRNA (C, areas of lesion), showing data for individual animals with mean value for each group (box).

    Techniques Used: Histopathology, In Situ Hybridization, Infection, Staining

    Images constructed from CT scans collected 18 days after challenge with SARS-CoV-2 showing pulmonary abnormalities in two cynomolgus (A, B) and one rhesus macaque (C). Arrows indicate areas of ground glass opacification and consolidation.
    Figure Legend Snippet: Images constructed from CT scans collected 18 days after challenge with SARS-CoV-2 showing pulmonary abnormalities in two cynomolgus (A, B) and one rhesus macaque (C). Arrows indicate areas of ground glass opacification and consolidation.

    Techniques Used: Construct

    SARS-CoV-2-specific IgG antibodies measured by ELISA in naïve and SARS-CoV-2 infected macaques. Spike- (A), Receptor-Binding Domain- (B) and Nucleoprotein- (C) specific IgG antibodies measured in sera of rhesus and cynomolgus macaques. Sera were collected from uninfected animals (day 0) or 1-3, 4-6, 8-9, 11-12 and 14-19 days following SARS-CoV-2 infection. Bars show the group mean +/- SEM with an endpoint titre determined for each individual animal shown as squares for males and dots for females. * p ≤ 0.05 (Kruskal-Wallis one-way ANOVA). Experiment performed in duplicates.
    Figure Legend Snippet: SARS-CoV-2-specific IgG antibodies measured by ELISA in naïve and SARS-CoV-2 infected macaques. Spike- (A), Receptor-Binding Domain- (B) and Nucleoprotein- (C) specific IgG antibodies measured in sera of rhesus and cynomolgus macaques. Sera were collected from uninfected animals (day 0) or 1-3, 4-6, 8-9, 11-12 and 14-19 days following SARS-CoV-2 infection. Bars show the group mean +/- SEM with an endpoint titre determined for each individual animal shown as squares for males and dots for females. * p ≤ 0.05 (Kruskal-Wallis one-way ANOVA). Experiment performed in duplicates.

    Techniques Used: Enzyme-linked Immunosorbent Assay, Infection, Binding Assay

    2) Product Images from "Immunological and pathological outcomes of SARS-CoV-2 challenge after formalin-inactivated vaccine immunisation of ferrets and rhesus macaques"

    Article Title: Immunological and pathological outcomes of SARS-CoV-2 challenge after formalin-inactivated vaccine immunisation of ferrets and rhesus macaques

    Journal: bioRxiv

    doi: 10.1101/2020.12.21.423746

    A: Heatmap showing the individual lung histopathology scores for each ferret and parameter following FIV- or Ad-GFP-vaccination, and challenged with SARS-CoV-2 and culled at 6/7 days and 13/15 days pc. Histopathology of FIV- (B-E) and Ad-GFP (F-I)-vaccinated ferrets. B. Inflammatory infiltrates within a bronchiole (*), with abundant mononuclear cells but also some neutrophils and eosinophils. H E, 200x. C. Multiple inflammatory infiltrates surrounding blood vessels (perivascular cuffing, arrows). H E, 100x. The infiltrates are composed mostly of macrophages and lymphocytes, but abundant eosinophils can also be observed in some areas within the infiltrates (insert; H E, 400x). D. A perivascular cuff (arrow) with abundant mononuclear cells, many of them identified as CD3 + T lymphocytes. IHC, 200x. E. Periportal mononuclear inflammatory infiltrate in the liver (mild multifocal hepatitis). H E, 400x. F. Mild inflammatory infiltrate within a bronchiole (*). H E, 200x. G. Blood vessel (arrow) within the lung parenchyma not showing any perivascular cuffing. H E, 200x. H. ISH detection of SARS-CoV-2 RNA in a small focus of epithelial and sustentacular cells within the nasal cavity. ISH, 200x. I. Periportal mononuclear inflammatory infiltrate in the liver (mild multifocal hepatitis). H E, 400x.
    Figure Legend Snippet: A: Heatmap showing the individual lung histopathology scores for each ferret and parameter following FIV- or Ad-GFP-vaccination, and challenged with SARS-CoV-2 and culled at 6/7 days and 13/15 days pc. Histopathology of FIV- (B-E) and Ad-GFP (F-I)-vaccinated ferrets. B. Inflammatory infiltrates within a bronchiole (*), with abundant mononuclear cells but also some neutrophils and eosinophils. H E, 200x. C. Multiple inflammatory infiltrates surrounding blood vessels (perivascular cuffing, arrows). H E, 100x. The infiltrates are composed mostly of macrophages and lymphocytes, but abundant eosinophils can also be observed in some areas within the infiltrates (insert; H E, 400x). D. A perivascular cuff (arrow) with abundant mononuclear cells, many of them identified as CD3 + T lymphocytes. IHC, 200x. E. Periportal mononuclear inflammatory infiltrate in the liver (mild multifocal hepatitis). H E, 400x. F. Mild inflammatory infiltrate within a bronchiole (*). H E, 200x. G. Blood vessel (arrow) within the lung parenchyma not showing any perivascular cuffing. H E, 200x. H. ISH detection of SARS-CoV-2 RNA in a small focus of epithelial and sustentacular cells within the nasal cavity. ISH, 200x. I. Periportal mononuclear inflammatory infiltrate in the liver (mild multifocal hepatitis). H E, 400x.

    Techniques Used: Histopathology, Immunohistochemistry, In Situ Hybridization

    Serological response in unvaccinated and FIV-vaccinated macaques. IgG was quantified by ELISA to recombinant nucleocapsid protein (NP), receptor binding domain (RBD) and full-length trimeric and stabilised spike protein (Spike). Bars are geometric mean titre. The significance of any difference from pre-to post-vaccination is shown, determined by a paired t-test. The micronutralisation 50% titre (ND 50 ) is also shown with samples obtained pre- and post-vaccination and following SARS-CoV-2 challenge. Bars are geometric mean ND 50 titre.
    Figure Legend Snippet: Serological response in unvaccinated and FIV-vaccinated macaques. IgG was quantified by ELISA to recombinant nucleocapsid protein (NP), receptor binding domain (RBD) and full-length trimeric and stabilised spike protein (Spike). Bars are geometric mean titre. The significance of any difference from pre-to post-vaccination is shown, determined by a paired t-test. The micronutralisation 50% titre (ND 50 ) is also shown with samples obtained pre- and post-vaccination and following SARS-CoV-2 challenge. Bars are geometric mean ND 50 titre.

    Techniques Used: Enzyme-linked Immunosorbent Assay, Recombinant, Binding Assay

    Serological response to Ad-GFP and FIV in ferrets. IgG was quantified by ELISA to recombinant nucleocapsid protein (NP), receptor binding domain (RBD) and full-length trimeric and stabilised spike protein (Spike). Bars are geometric mean titre. The significance of any difference from pre-to post-vaccination is shown, determined by a paired t-test. The plaque reduction neutralisation 50% titre (PRNT 50 ) is also shown with samples obtained pre- and post-vaccination and following SARS-CoV-2 challenge. Bars are geometric mean PRNT 50 titre.
    Figure Legend Snippet: Serological response to Ad-GFP and FIV in ferrets. IgG was quantified by ELISA to recombinant nucleocapsid protein (NP), receptor binding domain (RBD) and full-length trimeric and stabilised spike protein (Spike). Bars are geometric mean titre. The significance of any difference from pre-to post-vaccination is shown, determined by a paired t-test. The plaque reduction neutralisation 50% titre (PRNT 50 ) is also shown with samples obtained pre- and post-vaccination and following SARS-CoV-2 challenge. Bars are geometric mean PRNT 50 titre.

    Techniques Used: Enzyme-linked Immunosorbent Assay, Recombinant, Binding Assay, Plaque Reduction Neutralization Test

    Detection of SARS-CoV-2 RNA in macaque respiratory samples. Viral RNA in unvaccinated and FIV-vaccinated macaques was quantified by RT-PCR in (A) nasal washes, (B) throat swabs and (C) bronchiolar lavage. Lines plotted are the geometric mean genome copies per mL.
    Figure Legend Snippet: Detection of SARS-CoV-2 RNA in macaque respiratory samples. Viral RNA in unvaccinated and FIV-vaccinated macaques was quantified by RT-PCR in (A) nasal washes, (B) throat swabs and (C) bronchiolar lavage. Lines plotted are the geometric mean genome copies per mL.

    Techniques Used: Reverse Transcription Polymerase Chain Reaction

    Detection of SARS-CoV-2 RNA in ferret respiratory samples. Viral RNA in Ad-GFP and FIV-vaccinated ferrets was quantified by RT-PCR in (A) nasal washes and (B) throat swabs. Lines plotted are the geometric mean genome copies per mL.
    Figure Legend Snippet: Detection of SARS-CoV-2 RNA in ferret respiratory samples. Viral RNA in Ad-GFP and FIV-vaccinated ferrets was quantified by RT-PCR in (A) nasal washes and (B) throat swabs. Lines plotted are the geometric mean genome copies per mL.

    Techniques Used: Reverse Transcription Polymerase Chain Reaction

    Representative transmission electron microscopy images of (A) the initial SARS-CoV-2 virus preparation and (B) following formaldehyde inactivation and washing to remove medium constituents.
    Figure Legend Snippet: Representative transmission electron microscopy images of (A) the initial SARS-CoV-2 virus preparation and (B) following formaldehyde inactivation and washing to remove medium constituents.

    Techniques Used: Transmission Assay, Electron Microscopy

    Related Articles

    Recombinant:

    Article Title: Comparison of rhesus and cynomolgus macaques as an infection model for COVID-19
    Article Snippet: .. Recombinant SARS-CoV-2 Nucleocapsid phosphoprotein (GenBank: MN908947, isolate Wuhan-Hu-1) was expressed and purified from Escherichia coli as full-length nucleoprotein (amino acids 1-419) with a C-terminal 6xHis-Tag (REC31812-100, Batch #20042310, Native Antigen Company). ..

    Article Title: Aerosol Exposure of Cynomolgus Macaques to SARS-CoV-2 Results in More Severe Pathology than Existing Models
    Article Snippet: .. Recombinant SARS-CoV-2 full trimeric spike (gift from Dr. Jason McLellan’s group; UT-Austin) , RBD (Sino Biological, 40592-V08H), and NP (Native Antigen Company, REC31812-100) proteins were coupled to Magplex microsphere regions #45, #65, and #25 using the Luminex xMAP® antibody coupling kit (Luminex Inc., Austin, TX, USA) according to the manufacturer’s instructions. ..

    Article Title: Immunological and pathological outcomes of SARS-CoV-2 challenge after formalin-inactivated vaccine immunisation of ferrets and rhesus macaques
    Article Snippet: .. Recombinant SARS-CoV-2 Nucleocapsid phosphoprotein (GenBank: MN908947 , isolate Wuhan-Hu-1) was expressed and purified from Escherichia coli as full-length nucleoprotein (amino acids 1-419) with a C-terminal 6xHis-Tag (Native Antigen Company). ..

    Article Title: Comparison of Rhesus and Cynomolgus macaques as an authentic model for COVID-19
    Article Snippet: .. Recombinant SARS-CoV-2 Nucleocapsid phosphoprotein (GenBank: MN908947, isolate Wuhan-Hu-1) was expressed and purified from Escherichia coli as full-length nucleoprotein (amino acids 1-419) with a C-terminal 6xHis-Tag (REC31812-100, Batch #20042310, Native Antigen Company). ..

    Article Title: Intranasal Infection of Ferrets with SARS-CoV-2 as a Model for Asymptomatic Human Infection
    Article Snippet: .. Antibody titres to His-tag recombinant viral proteins spike subunit 1 (S1) (REC31828-100) and nucleoprotein (NP) (REC31812-100) (gifts from The Native Antigen Company, Oxford, UK) were determined by direct ELISA. ..

    Purification:

    Article Title: Comparison of rhesus and cynomolgus macaques as an infection model for COVID-19
    Article Snippet: .. Recombinant SARS-CoV-2 Nucleocapsid phosphoprotein (GenBank: MN908947, isolate Wuhan-Hu-1) was expressed and purified from Escherichia coli as full-length nucleoprotein (amino acids 1-419) with a C-terminal 6xHis-Tag (REC31812-100, Batch #20042310, Native Antigen Company). ..

    Article Title: Immunological and pathological outcomes of SARS-CoV-2 challenge after formalin-inactivated vaccine immunisation of ferrets and rhesus macaques
    Article Snippet: .. Recombinant SARS-CoV-2 Nucleocapsid phosphoprotein (GenBank: MN908947 , isolate Wuhan-Hu-1) was expressed and purified from Escherichia coli as full-length nucleoprotein (amino acids 1-419) with a C-terminal 6xHis-Tag (Native Antigen Company). ..

    Article Title: Comparison of Rhesus and Cynomolgus macaques as an authentic model for COVID-19
    Article Snippet: .. Recombinant SARS-CoV-2 Nucleocapsid phosphoprotein (GenBank: MN908947, isolate Wuhan-Hu-1) was expressed and purified from Escherichia coli as full-length nucleoprotein (amino acids 1-419) with a C-terminal 6xHis-Tag (REC31812-100, Batch #20042310, Native Antigen Company). ..

    Direct ELISA:

    Article Title: Intranasal Infection of Ferrets with SARS-CoV-2 as a Model for Asymptomatic Human Infection
    Article Snippet: .. Antibody titres to His-tag recombinant viral proteins spike subunit 1 (S1) (REC31828-100) and nucleoprotein (NP) (REC31812-100) (gifts from The Native Antigen Company, Oxford, UK) were determined by direct ELISA. ..

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    Native Antigen Inc recombinant sars cov 2 nucleocapsid phosphoprotein
    Cellular immune responses to <t>SARS-CoV-2.</t> A, B) IFNγ SFU measured in PBMCs and stimulated with spike protein peptide pools (PP) peptide in A) rhesus and B) cynomolgus macaques. PBMC samples were isolated from uninfected animals (naïve) or at early (days 4 and 5) and late (days 14-19) time-points following SARS-CoV-2 infection. Box plots show the group median +/- inter-quartile range, with minimum and maximum values connected by whiskers. C, D) IFNγ SFU measured in PBMC in response to spike protein megapools (MP) in C) rhesus and D) cynomolgus macaques or, E) in mononuclear cells isolated from lung and spleen. Bars show the group median with SFU measured in individual animals shown as dots. * p ≤ 0.05 , ** p ≤ 0.01. F-J) Frequency of major lymphocyte and monocyte cell populations quantified by immunophenotyping assay F - H) CD4+, CD8+ and γδ T-cell frequencies in PBMCs and lung cells, I) Monocyte subtype frequency in PBMCs and lung MNCs, J) Natural killer (NK) cell subset frequency in PBMCs and lung MNCs. Stacked bars show the group median with 95% confidence intervals. PBMC: Naïve rhesus n=8, early rhesus n= 1, late rhesus n=2, naïve cyno = 7, early cyno n=2, late cyno n=2. Lung: early rhesus n= 2, late rhesus n=3, early cyno n=2, late cyno n=2. K-N) Intracellular cytokine staining data K-L) Cytokine and activation marker detection in CD4+, CD8+ and γδ T-cells in PBMCs stimulated with M, N and S peptide pools. G-N) CD107a expression in CD8+ and γδ T-cells in PBMCs. Bars show the group median with cell frequencies measured in individual animals shown as dots.
    Recombinant Sars Cov 2 Nucleocapsid Phosphoprotein, supplied by Native Antigen Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant sars cov 2 nucleocapsid phosphoprotein/product/Native Antigen Inc
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant sars cov 2 nucleocapsid phosphoprotein - by Bioz Stars, 2021-09
    95/100 stars
      Buy from Supplier

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    Cellular immune responses to SARS-CoV-2. A, B) IFNγ SFU measured in PBMCs and stimulated with spike protein peptide pools (PP) peptide in A) rhesus and B) cynomolgus macaques. PBMC samples were isolated from uninfected animals (naïve) or at early (days 4 and 5) and late (days 14-19) time-points following SARS-CoV-2 infection. Box plots show the group median +/- inter-quartile range, with minimum and maximum values connected by whiskers. C, D) IFNγ SFU measured in PBMC in response to spike protein megapools (MP) in C) rhesus and D) cynomolgus macaques or, E) in mononuclear cells isolated from lung and spleen. Bars show the group median with SFU measured in individual animals shown as dots. * p ≤ 0.05 , ** p ≤ 0.01. F-J) Frequency of major lymphocyte and monocyte cell populations quantified by immunophenotyping assay F - H) CD4+, CD8+ and γδ T-cell frequencies in PBMCs and lung cells, I) Monocyte subtype frequency in PBMCs and lung MNCs, J) Natural killer (NK) cell subset frequency in PBMCs and lung MNCs. Stacked bars show the group median with 95% confidence intervals. PBMC: Naïve rhesus n=8, early rhesus n= 1, late rhesus n=2, naïve cyno = 7, early cyno n=2, late cyno n=2. Lung: early rhesus n= 2, late rhesus n=3, early cyno n=2, late cyno n=2. K-N) Intracellular cytokine staining data K-L) Cytokine and activation marker detection in CD4+, CD8+ and γδ T-cells in PBMCs stimulated with M, N and S peptide pools. G-N) CD107a expression in CD8+ and γδ T-cells in PBMCs. Bars show the group median with cell frequencies measured in individual animals shown as dots.

    Journal: bioRxiv

    Article Title: Comparison of Rhesus and Cynomolgus macaques as an authentic model for COVID-19

    doi: 10.1101/2020.09.17.301093

    Figure Lengend Snippet: Cellular immune responses to SARS-CoV-2. A, B) IFNγ SFU measured in PBMCs and stimulated with spike protein peptide pools (PP) peptide in A) rhesus and B) cynomolgus macaques. PBMC samples were isolated from uninfected animals (naïve) or at early (days 4 and 5) and late (days 14-19) time-points following SARS-CoV-2 infection. Box plots show the group median +/- inter-quartile range, with minimum and maximum values connected by whiskers. C, D) IFNγ SFU measured in PBMC in response to spike protein megapools (MP) in C) rhesus and D) cynomolgus macaques or, E) in mononuclear cells isolated from lung and spleen. Bars show the group median with SFU measured in individual animals shown as dots. * p ≤ 0.05 , ** p ≤ 0.01. F-J) Frequency of major lymphocyte and monocyte cell populations quantified by immunophenotyping assay F - H) CD4+, CD8+ and γδ T-cell frequencies in PBMCs and lung cells, I) Monocyte subtype frequency in PBMCs and lung MNCs, J) Natural killer (NK) cell subset frequency in PBMCs and lung MNCs. Stacked bars show the group median with 95% confidence intervals. PBMC: Naïve rhesus n=8, early rhesus n= 1, late rhesus n=2, naïve cyno = 7, early cyno n=2, late cyno n=2. Lung: early rhesus n= 2, late rhesus n=3, early cyno n=2, late cyno n=2. K-N) Intracellular cytokine staining data K-L) Cytokine and activation marker detection in CD4+, CD8+ and γδ T-cells in PBMCs stimulated with M, N and S peptide pools. G-N) CD107a expression in CD8+ and γδ T-cells in PBMCs. Bars show the group median with cell frequencies measured in individual animals shown as dots.

    Article Snippet: Recombinant SARS-CoV-2 Nucleocapsid phosphoprotein (GenBank: MN908947, isolate Wuhan-Hu-1) was expressed and purified from Escherichia coli as full-length nucleoprotein (amino acids 1-419) with a C-terminal 6xHis-Tag (REC31812-100, Batch #20042310, Native Antigen Company).

    Techniques: Isolation, Infection, Staining, Activation Assay, Marker, Expressing

    Histopathological changes in cynomolgus and rhesus macaques during SARS-CoV-2 infection. Diffuse areas of DAD observed in cynomolgus macaques at 4/5 dpc with shrunken, eosinophilic cells within the alveolar walls (A, B), together with alveolar oedema (A, arrows) pneumocyte hyperplasia and expanded alveolar spaces with inflammatory cell infiltration (B, arrows). Occasional multinucleated cells resembling syncytial cells are observed (B, insert). ISH detection of viral RNA (RNAScope, red chromogen) within the areas of pneumonia (C) and occasionally in the BALT (C, insert). Abundant IL-6 producing cells observed in the areas of pneumonia (D) Similar histopathological changes observed in rhesus macaques, including DAD areas with patchy alveolar oedema (E, arrow), alveolar macrophage hyperplasia (F, arrow), bronchial exudates and presence of viral RNA within the areas showing pneumonia (G) and abundant IL-6 producing cells (H). Histopathological changes with less severity observed at 14/15 dpc in cynomolgus macaques, with infiltration of mononuclear cells within alveolar spaces and bronchiolar luminae (I, arrows) and parenchymal collapse (I, *) and perivascular cuffing (J, arrow), with minimal detection of viral RNA in pneumocytes (J, insert). Bronchiole regeneration (K, arrow) and perivascular/peribronchiolar cuffing observed in rhesus macaques at 14/15 dpc (L, arrows), together with BALT proliferation (L, *) with minimal presence of viral RNA (L, insert).

    Journal: bioRxiv

    Article Title: Comparison of Rhesus and Cynomolgus macaques as an authentic model for COVID-19

    doi: 10.1101/2020.09.17.301093

    Figure Lengend Snippet: Histopathological changes in cynomolgus and rhesus macaques during SARS-CoV-2 infection. Diffuse areas of DAD observed in cynomolgus macaques at 4/5 dpc with shrunken, eosinophilic cells within the alveolar walls (A, B), together with alveolar oedema (A, arrows) pneumocyte hyperplasia and expanded alveolar spaces with inflammatory cell infiltration (B, arrows). Occasional multinucleated cells resembling syncytial cells are observed (B, insert). ISH detection of viral RNA (RNAScope, red chromogen) within the areas of pneumonia (C) and occasionally in the BALT (C, insert). Abundant IL-6 producing cells observed in the areas of pneumonia (D) Similar histopathological changes observed in rhesus macaques, including DAD areas with patchy alveolar oedema (E, arrow), alveolar macrophage hyperplasia (F, arrow), bronchial exudates and presence of viral RNA within the areas showing pneumonia (G) and abundant IL-6 producing cells (H). Histopathological changes with less severity observed at 14/15 dpc in cynomolgus macaques, with infiltration of mononuclear cells within alveolar spaces and bronchiolar luminae (I, arrows) and parenchymal collapse (I, *) and perivascular cuffing (J, arrow), with minimal detection of viral RNA in pneumocytes (J, insert). Bronchiole regeneration (K, arrow) and perivascular/peribronchiolar cuffing observed in rhesus macaques at 14/15 dpc (L, arrows), together with BALT proliferation (L, *) with minimal presence of viral RNA (L, insert).

    Article Snippet: Recombinant SARS-CoV-2 Nucleocapsid phosphoprotein (GenBank: MN908947, isolate Wuhan-Hu-1) was expressed and purified from Escherichia coli as full-length nucleoprotein (amino acids 1-419) with a C-terminal 6xHis-Tag (REC31812-100, Batch #20042310, Native Antigen Company).

    Techniques: Infection, In Situ Hybridization

    Lung histopathology scores and presence of viral RNA and IL-6 by ISH in cynomolgus and rhesus macaques during SARS-CoV-2 infection. Heatmap showing the individual and aggregate (TOTAL) scores for each lung histopathological parameter and animal (A). Image analysis of positively stained area in RNASCope labelled sections for viral RNA (B; whole slide) and IL-6 mRNA (C, areas of lesion), showing data for individual animals with mean value for each group (box).

    Journal: bioRxiv

    Article Title: Comparison of Rhesus and Cynomolgus macaques as an authentic model for COVID-19

    doi: 10.1101/2020.09.17.301093

    Figure Lengend Snippet: Lung histopathology scores and presence of viral RNA and IL-6 by ISH in cynomolgus and rhesus macaques during SARS-CoV-2 infection. Heatmap showing the individual and aggregate (TOTAL) scores for each lung histopathological parameter and animal (A). Image analysis of positively stained area in RNASCope labelled sections for viral RNA (B; whole slide) and IL-6 mRNA (C, areas of lesion), showing data for individual animals with mean value for each group (box).

    Article Snippet: Recombinant SARS-CoV-2 Nucleocapsid phosphoprotein (GenBank: MN908947, isolate Wuhan-Hu-1) was expressed and purified from Escherichia coli as full-length nucleoprotein (amino acids 1-419) with a C-terminal 6xHis-Tag (REC31812-100, Batch #20042310, Native Antigen Company).

    Techniques: Histopathology, In Situ Hybridization, Infection, Staining

    Images constructed from CT scans collected 18 days after challenge with SARS-CoV-2 showing pulmonary abnormalities in two cynomolgus (A, B) and one rhesus macaque (C). Arrows indicate areas of ground glass opacification and consolidation.

    Journal: bioRxiv

    Article Title: Comparison of Rhesus and Cynomolgus macaques as an authentic model for COVID-19

    doi: 10.1101/2020.09.17.301093

    Figure Lengend Snippet: Images constructed from CT scans collected 18 days after challenge with SARS-CoV-2 showing pulmonary abnormalities in two cynomolgus (A, B) and one rhesus macaque (C). Arrows indicate areas of ground glass opacification and consolidation.

    Article Snippet: Recombinant SARS-CoV-2 Nucleocapsid phosphoprotein (GenBank: MN908947, isolate Wuhan-Hu-1) was expressed and purified from Escherichia coli as full-length nucleoprotein (amino acids 1-419) with a C-terminal 6xHis-Tag (REC31812-100, Batch #20042310, Native Antigen Company).

    Techniques: Construct

    SARS-CoV-2-specific IgG antibodies measured by ELISA in naïve and SARS-CoV-2 infected macaques. Spike- (A), Receptor-Binding Domain- (B) and Nucleoprotein- (C) specific IgG antibodies measured in sera of rhesus and cynomolgus macaques. Sera were collected from uninfected animals (day 0) or 1-3, 4-6, 8-9, 11-12 and 14-19 days following SARS-CoV-2 infection. Bars show the group mean +/- SEM with an endpoint titre determined for each individual animal shown as squares for males and dots for females. * p ≤ 0.05 (Kruskal-Wallis one-way ANOVA). Experiment performed in duplicates.

    Journal: bioRxiv

    Article Title: Comparison of Rhesus and Cynomolgus macaques as an authentic model for COVID-19

    doi: 10.1101/2020.09.17.301093

    Figure Lengend Snippet: SARS-CoV-2-specific IgG antibodies measured by ELISA in naïve and SARS-CoV-2 infected macaques. Spike- (A), Receptor-Binding Domain- (B) and Nucleoprotein- (C) specific IgG antibodies measured in sera of rhesus and cynomolgus macaques. Sera were collected from uninfected animals (day 0) or 1-3, 4-6, 8-9, 11-12 and 14-19 days following SARS-CoV-2 infection. Bars show the group mean +/- SEM with an endpoint titre determined for each individual animal shown as squares for males and dots for females. * p ≤ 0.05 (Kruskal-Wallis one-way ANOVA). Experiment performed in duplicates.

    Article Snippet: Recombinant SARS-CoV-2 Nucleocapsid phosphoprotein (GenBank: MN908947, isolate Wuhan-Hu-1) was expressed and purified from Escherichia coli as full-length nucleoprotein (amino acids 1-419) with a C-terminal 6xHis-Tag (REC31812-100, Batch #20042310, Native Antigen Company).

    Techniques: Enzyme-linked Immunosorbent Assay, Infection, Binding Assay

    A: Heatmap showing the individual lung histopathology scores for each ferret and parameter following FIV- or Ad-GFP-vaccination, and challenged with SARS-CoV-2 and culled at 6/7 days and 13/15 days pc. Histopathology of FIV- (B-E) and Ad-GFP (F-I)-vaccinated ferrets. B. Inflammatory infiltrates within a bronchiole (*), with abundant mononuclear cells but also some neutrophils and eosinophils. H E, 200x. C. Multiple inflammatory infiltrates surrounding blood vessels (perivascular cuffing, arrows). H E, 100x. The infiltrates are composed mostly of macrophages and lymphocytes, but abundant eosinophils can also be observed in some areas within the infiltrates (insert; H E, 400x). D. A perivascular cuff (arrow) with abundant mononuclear cells, many of them identified as CD3 + T lymphocytes. IHC, 200x. E. Periportal mononuclear inflammatory infiltrate in the liver (mild multifocal hepatitis). H E, 400x. F. Mild inflammatory infiltrate within a bronchiole (*). H E, 200x. G. Blood vessel (arrow) within the lung parenchyma not showing any perivascular cuffing. H E, 200x. H. ISH detection of SARS-CoV-2 RNA in a small focus of epithelial and sustentacular cells within the nasal cavity. ISH, 200x. I. Periportal mononuclear inflammatory infiltrate in the liver (mild multifocal hepatitis). H E, 400x.

    Journal: bioRxiv

    Article Title: Immunological and pathological outcomes of SARS-CoV-2 challenge after formalin-inactivated vaccine immunisation of ferrets and rhesus macaques

    doi: 10.1101/2020.12.21.423746

    Figure Lengend Snippet: A: Heatmap showing the individual lung histopathology scores for each ferret and parameter following FIV- or Ad-GFP-vaccination, and challenged with SARS-CoV-2 and culled at 6/7 days and 13/15 days pc. Histopathology of FIV- (B-E) and Ad-GFP (F-I)-vaccinated ferrets. B. Inflammatory infiltrates within a bronchiole (*), with abundant mononuclear cells but also some neutrophils and eosinophils. H E, 200x. C. Multiple inflammatory infiltrates surrounding blood vessels (perivascular cuffing, arrows). H E, 100x. The infiltrates are composed mostly of macrophages and lymphocytes, but abundant eosinophils can also be observed in some areas within the infiltrates (insert; H E, 400x). D. A perivascular cuff (arrow) with abundant mononuclear cells, many of them identified as CD3 + T lymphocytes. IHC, 200x. E. Periportal mononuclear inflammatory infiltrate in the liver (mild multifocal hepatitis). H E, 400x. F. Mild inflammatory infiltrate within a bronchiole (*). H E, 200x. G. Blood vessel (arrow) within the lung parenchyma not showing any perivascular cuffing. H E, 200x. H. ISH detection of SARS-CoV-2 RNA in a small focus of epithelial and sustentacular cells within the nasal cavity. ISH, 200x. I. Periportal mononuclear inflammatory infiltrate in the liver (mild multifocal hepatitis). H E, 400x.

    Article Snippet: Recombinant SARS-CoV-2 Nucleocapsid phosphoprotein (GenBank: MN908947 , isolate Wuhan-Hu-1) was expressed and purified from Escherichia coli as full-length nucleoprotein (amino acids 1-419) with a C-terminal 6xHis-Tag (Native Antigen Company).

    Techniques: Histopathology, Immunohistochemistry, In Situ Hybridization

    Serological response in unvaccinated and FIV-vaccinated macaques. IgG was quantified by ELISA to recombinant nucleocapsid protein (NP), receptor binding domain (RBD) and full-length trimeric and stabilised spike protein (Spike). Bars are geometric mean titre. The significance of any difference from pre-to post-vaccination is shown, determined by a paired t-test. The micronutralisation 50% titre (ND 50 ) is also shown with samples obtained pre- and post-vaccination and following SARS-CoV-2 challenge. Bars are geometric mean ND 50 titre.

    Journal: bioRxiv

    Article Title: Immunological and pathological outcomes of SARS-CoV-2 challenge after formalin-inactivated vaccine immunisation of ferrets and rhesus macaques

    doi: 10.1101/2020.12.21.423746

    Figure Lengend Snippet: Serological response in unvaccinated and FIV-vaccinated macaques. IgG was quantified by ELISA to recombinant nucleocapsid protein (NP), receptor binding domain (RBD) and full-length trimeric and stabilised spike protein (Spike). Bars are geometric mean titre. The significance of any difference from pre-to post-vaccination is shown, determined by a paired t-test. The micronutralisation 50% titre (ND 50 ) is also shown with samples obtained pre- and post-vaccination and following SARS-CoV-2 challenge. Bars are geometric mean ND 50 titre.

    Article Snippet: Recombinant SARS-CoV-2 Nucleocapsid phosphoprotein (GenBank: MN908947 , isolate Wuhan-Hu-1) was expressed and purified from Escherichia coli as full-length nucleoprotein (amino acids 1-419) with a C-terminal 6xHis-Tag (Native Antigen Company).

    Techniques: Enzyme-linked Immunosorbent Assay, Recombinant, Binding Assay

    Serological response to Ad-GFP and FIV in ferrets. IgG was quantified by ELISA to recombinant nucleocapsid protein (NP), receptor binding domain (RBD) and full-length trimeric and stabilised spike protein (Spike). Bars are geometric mean titre. The significance of any difference from pre-to post-vaccination is shown, determined by a paired t-test. The plaque reduction neutralisation 50% titre (PRNT 50 ) is also shown with samples obtained pre- and post-vaccination and following SARS-CoV-2 challenge. Bars are geometric mean PRNT 50 titre.

    Journal: bioRxiv

    Article Title: Immunological and pathological outcomes of SARS-CoV-2 challenge after formalin-inactivated vaccine immunisation of ferrets and rhesus macaques

    doi: 10.1101/2020.12.21.423746

    Figure Lengend Snippet: Serological response to Ad-GFP and FIV in ferrets. IgG was quantified by ELISA to recombinant nucleocapsid protein (NP), receptor binding domain (RBD) and full-length trimeric and stabilised spike protein (Spike). Bars are geometric mean titre. The significance of any difference from pre-to post-vaccination is shown, determined by a paired t-test. The plaque reduction neutralisation 50% titre (PRNT 50 ) is also shown with samples obtained pre- and post-vaccination and following SARS-CoV-2 challenge. Bars are geometric mean PRNT 50 titre.

    Article Snippet: Recombinant SARS-CoV-2 Nucleocapsid phosphoprotein (GenBank: MN908947 , isolate Wuhan-Hu-1) was expressed and purified from Escherichia coli as full-length nucleoprotein (amino acids 1-419) with a C-terminal 6xHis-Tag (Native Antigen Company).

    Techniques: Enzyme-linked Immunosorbent Assay, Recombinant, Binding Assay, Plaque Reduction Neutralization Test

    Detection of SARS-CoV-2 RNA in macaque respiratory samples. Viral RNA in unvaccinated and FIV-vaccinated macaques was quantified by RT-PCR in (A) nasal washes, (B) throat swabs and (C) bronchiolar lavage. Lines plotted are the geometric mean genome copies per mL.

    Journal: bioRxiv

    Article Title: Immunological and pathological outcomes of SARS-CoV-2 challenge after formalin-inactivated vaccine immunisation of ferrets and rhesus macaques

    doi: 10.1101/2020.12.21.423746

    Figure Lengend Snippet: Detection of SARS-CoV-2 RNA in macaque respiratory samples. Viral RNA in unvaccinated and FIV-vaccinated macaques was quantified by RT-PCR in (A) nasal washes, (B) throat swabs and (C) bronchiolar lavage. Lines plotted are the geometric mean genome copies per mL.

    Article Snippet: Recombinant SARS-CoV-2 Nucleocapsid phosphoprotein (GenBank: MN908947 , isolate Wuhan-Hu-1) was expressed and purified from Escherichia coli as full-length nucleoprotein (amino acids 1-419) with a C-terminal 6xHis-Tag (Native Antigen Company).

    Techniques: Reverse Transcription Polymerase Chain Reaction

    Detection of SARS-CoV-2 RNA in ferret respiratory samples. Viral RNA in Ad-GFP and FIV-vaccinated ferrets was quantified by RT-PCR in (A) nasal washes and (B) throat swabs. Lines plotted are the geometric mean genome copies per mL.

    Journal: bioRxiv

    Article Title: Immunological and pathological outcomes of SARS-CoV-2 challenge after formalin-inactivated vaccine immunisation of ferrets and rhesus macaques

    doi: 10.1101/2020.12.21.423746

    Figure Lengend Snippet: Detection of SARS-CoV-2 RNA in ferret respiratory samples. Viral RNA in Ad-GFP and FIV-vaccinated ferrets was quantified by RT-PCR in (A) nasal washes and (B) throat swabs. Lines plotted are the geometric mean genome copies per mL.

    Article Snippet: Recombinant SARS-CoV-2 Nucleocapsid phosphoprotein (GenBank: MN908947 , isolate Wuhan-Hu-1) was expressed and purified from Escherichia coli as full-length nucleoprotein (amino acids 1-419) with a C-terminal 6xHis-Tag (Native Antigen Company).

    Techniques: Reverse Transcription Polymerase Chain Reaction

    Representative transmission electron microscopy images of (A) the initial SARS-CoV-2 virus preparation and (B) following formaldehyde inactivation and washing to remove medium constituents.

    Journal: bioRxiv

    Article Title: Immunological and pathological outcomes of SARS-CoV-2 challenge after formalin-inactivated vaccine immunisation of ferrets and rhesus macaques

    doi: 10.1101/2020.12.21.423746

    Figure Lengend Snippet: Representative transmission electron microscopy images of (A) the initial SARS-CoV-2 virus preparation and (B) following formaldehyde inactivation and washing to remove medium constituents.

    Article Snippet: Recombinant SARS-CoV-2 Nucleocapsid phosphoprotein (GenBank: MN908947 , isolate Wuhan-Hu-1) was expressed and purified from Escherichia coli as full-length nucleoprotein (amino acids 1-419) with a C-terminal 6xHis-Tag (Native Antigen Company).

    Techniques: Transmission Assay, Electron Microscopy