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Cusabio recombinant rat cygb
<t>LPS-induced</t> tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) concentrations in supernatants of rat preoptic area (POA) primary cultures under the influence of <t>Cygb.</t> POA primary cultures cultured on poly-L-lysine-coated glass coverslips, were incubated for 240 min with fresh medium containing PBS (negative control), LPS at the concentration of 10 μg/ml (positive control) or LPS (10 μg/ml) plus Cygb (10 μg/ml or 20 μg/ml). LPS caused a significant increase in TNF-α and IL-6 concentrations in the supernatants of POA primary cultures and the co-treatment with Cygb prevent significantly this increase at the dose 20 μg/ml for TNF-α (A) and IL-6 (B) . The viability of the cells is not altered in any tested group (C) . Columns (means of 3–4 samples from three to six independent experiments) represent means with SEM (significant difference vs. LPS control group; * p
Recombinant Rat Cygb, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant rat cygb/product/Cusabio
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
recombinant rat cygb - by Bioz Stars, 2020-08
93/100 stars

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1) Product Images from "Cytoglobin Attenuates Neuroinflammation in Lipopolysaccharide-Activated Primary Preoptic Area Cells via NF-κB Pathway Inhibition"

Article Title: Cytoglobin Attenuates Neuroinflammation in Lipopolysaccharide-Activated Primary Preoptic Area Cells via NF-κB Pathway Inhibition

Journal: Frontiers in Molecular Neuroscience

doi: 10.3389/fnmol.2019.00307

LPS-induced tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) concentrations in supernatants of rat preoptic area (POA) primary cultures under the influence of Cygb. POA primary cultures cultured on poly-L-lysine-coated glass coverslips, were incubated for 240 min with fresh medium containing PBS (negative control), LPS at the concentration of 10 μg/ml (positive control) or LPS (10 μg/ml) plus Cygb (10 μg/ml or 20 μg/ml). LPS caused a significant increase in TNF-α and IL-6 concentrations in the supernatants of POA primary cultures and the co-treatment with Cygb prevent significantly this increase at the dose 20 μg/ml for TNF-α (A) and IL-6 (B) . The viability of the cells is not altered in any tested group (C) . Columns (means of 3–4 samples from three to six independent experiments) represent means with SEM (significant difference vs. LPS control group; * p
Figure Legend Snippet: LPS-induced tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) concentrations in supernatants of rat preoptic area (POA) primary cultures under the influence of Cygb. POA primary cultures cultured on poly-L-lysine-coated glass coverslips, were incubated for 240 min with fresh medium containing PBS (negative control), LPS at the concentration of 10 μg/ml (positive control) or LPS (10 μg/ml) plus Cygb (10 μg/ml or 20 μg/ml). LPS caused a significant increase in TNF-α and IL-6 concentrations in the supernatants of POA primary cultures and the co-treatment with Cygb prevent significantly this increase at the dose 20 μg/ml for TNF-α (A) and IL-6 (B) . The viability of the cells is not altered in any tested group (C) . Columns (means of 3–4 samples from three to six independent experiments) represent means with SEM (significant difference vs. LPS control group; * p

Techniques Used: Cell Culture, Incubation, Negative Control, Concentration Assay, Positive Control

Cygb does not affect the nuclear NF-IL6 and STAT3 immunoreactivity in microglia and astrocytes, respectively. Immunocytochemistry was proceeded in coverslips using the NF-IL6 antiserum in ED1-positive microglia (A,C) and STAT3 antiserum in GFAP-positive astrocytes (B,D) . The immunoreactivity was enhanced in the cells treated with LPS (10 μg/ml) compared to the PBS group and the co-treatment with Cygb (10 μg/ml or 20 μg/ml) does not affect this. In panel (C) , triple labeling for ED-1 (in green), NF-IL-6 (in red) and cellular nuclei by DAPI (in blue) allowed localization of NF-IL6 immunoreactivity in the nuclei of microglial cells. In panel (D) , triple labeling for GFAP (in green), STAT3 (in red) and cellular nuclei by DAPI (in blue) allowed localization of STAT3 immunoreactivity in the nuclei of astrocytes. Secondary antisera employed were coupled to fluorophores Alexa-488 (green label) and Cy3 (red label). Scale bars: 20 μm. The average intensities of the signals within the active region of interest (here: cell nuclei) were expressed as gray values. Columns represent means of the intensities measured in treated cultures derived from two independent preparations (*** p
Figure Legend Snippet: Cygb does not affect the nuclear NF-IL6 and STAT3 immunoreactivity in microglia and astrocytes, respectively. Immunocytochemistry was proceeded in coverslips using the NF-IL6 antiserum in ED1-positive microglia (A,C) and STAT3 antiserum in GFAP-positive astrocytes (B,D) . The immunoreactivity was enhanced in the cells treated with LPS (10 μg/ml) compared to the PBS group and the co-treatment with Cygb (10 μg/ml or 20 μg/ml) does not affect this. In panel (C) , triple labeling for ED-1 (in green), NF-IL-6 (in red) and cellular nuclei by DAPI (in blue) allowed localization of NF-IL6 immunoreactivity in the nuclei of microglial cells. In panel (D) , triple labeling for GFAP (in green), STAT3 (in red) and cellular nuclei by DAPI (in blue) allowed localization of STAT3 immunoreactivity in the nuclei of astrocytes. Secondary antisera employed were coupled to fluorophores Alexa-488 (green label) and Cy3 (red label). Scale bars: 20 μm. The average intensities of the signals within the active region of interest (here: cell nuclei) were expressed as gray values. Columns represent means of the intensities measured in treated cultures derived from two independent preparations (*** p

Techniques Used: Immunocytochemistry, Labeling, Derivative Assay

Nuclear factor-κB (NF-κB) immunoreactivity in microglia in the primary microculture of rat POA in the response of stimulation of LPS and treatment with Cygb. NF-κB immunoreactivity was detected in ED1-positive microglia after 240 min stimulation of primary cultures with medium containing 10 μg/ml LPS (C) , while the immunoreactivity in the PBS (A) groups was discrete. Triple labeling for ED-1 (in green), NF-κB (in red) and cellular nuclei by DAPI (in blue) demonstrate localization of NF-κB mainly to the nucleus of LPS-treated ED1-positive cells (C) , while there was a decrease NF-κB immunoreactivity in the cells treated with Cygb (20 μg/ml; E) . There are no significant changes in immunoreactivity in PBS + Cygb 20 μg or LPS + Cygb 10 μg groups ( B,D , respectively). Secondary antisera employed were coupled to fluorophores Alexa-488 (green label) and Cy3 (red label). Scale bars: 20 μm. The average intensities of the signals within the active region of interest (here: cell nuclei) were expressed as gray values (F) . Columns represent means of the intensities measured in treated cultures derived from two independent preparations (** p
Figure Legend Snippet: Nuclear factor-κB (NF-κB) immunoreactivity in microglia in the primary microculture of rat POA in the response of stimulation of LPS and treatment with Cygb. NF-κB immunoreactivity was detected in ED1-positive microglia after 240 min stimulation of primary cultures with medium containing 10 μg/ml LPS (C) , while the immunoreactivity in the PBS (A) groups was discrete. Triple labeling for ED-1 (in green), NF-κB (in red) and cellular nuclei by DAPI (in blue) demonstrate localization of NF-κB mainly to the nucleus of LPS-treated ED1-positive cells (C) , while there was a decrease NF-κB immunoreactivity in the cells treated with Cygb (20 μg/ml; E) . There are no significant changes in immunoreactivity in PBS + Cygb 20 μg or LPS + Cygb 10 μg groups ( B,D , respectively). Secondary antisera employed were coupled to fluorophores Alexa-488 (green label) and Cy3 (red label). Scale bars: 20 μm. The average intensities of the signals within the active region of interest (here: cell nuclei) were expressed as gray values (F) . Columns represent means of the intensities measured in treated cultures derived from two independent preparations (** p

Techniques Used: Labeling, Derivative Assay

Related Articles

Incubation:

Article Title: Cytoglobin Attenuates Neuroinflammation in Lipopolysaccharide-Activated Primary Preoptic Area Cells via NF-κB Pathway Inhibition
Article Snippet: .. To stimulate the cells, they were incubated with LPS (Sigma–Aldrich, St. Louis, MO, USA; 10 μg/ml) or recombinant rat Cygb (Cusabio Technology, Houston, TX, USA; 10 and 20 μg/ml) for 4 h. The concentrations of LPS and Cygb used were based on previous studies (Wen et al., ; Leisengang et al., ). .. PBS was used as a negative control.

Recombinant:

Article Title: Cytoglobin Attenuates Neuroinflammation in Lipopolysaccharide-Activated Primary Preoptic Area Cells via NF-κB Pathway Inhibition
Article Snippet: .. To stimulate the cells, they were incubated with LPS (Sigma–Aldrich, St. Louis, MO, USA; 10 μg/ml) or recombinant rat Cygb (Cusabio Technology, Houston, TX, USA; 10 and 20 μg/ml) for 4 h. The concentrations of LPS and Cygb used were based on previous studies (Wen et al., ; Leisengang et al., ). .. PBS was used as a negative control.

Article Title: Cytoglobin Attenuates Neuroinflammation in Lipopolysaccharide-Activated Primary Preoptic Area Cells via NF-κB Pathway Inhibition
Article Snippet: .. Since the recombinant rat Cygb used in the present study was expressed in E. coli , we cannot exclude the possibility that some residual level of endotoxin was still present and caused the effect of Cygb on nuclear immunoreactivity of transcription factors. ..

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    Cusabio recombinant rat cygb
    <t>LPS-induced</t> tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) concentrations in supernatants of rat preoptic area (POA) primary cultures under the influence of <t>Cygb.</t> POA primary cultures cultured on poly-L-lysine-coated glass coverslips, were incubated for 240 min with fresh medium containing PBS (negative control), LPS at the concentration of 10 μg/ml (positive control) or LPS (10 μg/ml) plus Cygb (10 μg/ml or 20 μg/ml). LPS caused a significant increase in TNF-α and IL-6 concentrations in the supernatants of POA primary cultures and the co-treatment with Cygb prevent significantly this increase at the dose 20 μg/ml for TNF-α (A) and IL-6 (B) . The viability of the cells is not altered in any tested group (C) . Columns (means of 3–4 samples from three to six independent experiments) represent means with SEM (significant difference vs. LPS control group; * p
    Recombinant Rat Cygb, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant rat cygb/product/Cusabio
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant rat cygb - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

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    LPS-induced tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) concentrations in supernatants of rat preoptic area (POA) primary cultures under the influence of Cygb. POA primary cultures cultured on poly-L-lysine-coated glass coverslips, were incubated for 240 min with fresh medium containing PBS (negative control), LPS at the concentration of 10 μg/ml (positive control) or LPS (10 μg/ml) plus Cygb (10 μg/ml or 20 μg/ml). LPS caused a significant increase in TNF-α and IL-6 concentrations in the supernatants of POA primary cultures and the co-treatment with Cygb prevent significantly this increase at the dose 20 μg/ml for TNF-α (A) and IL-6 (B) . The viability of the cells is not altered in any tested group (C) . Columns (means of 3–4 samples from three to six independent experiments) represent means with SEM (significant difference vs. LPS control group; * p

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Cytoglobin Attenuates Neuroinflammation in Lipopolysaccharide-Activated Primary Preoptic Area Cells via NF-κB Pathway Inhibition

    doi: 10.3389/fnmol.2019.00307

    Figure Lengend Snippet: LPS-induced tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) concentrations in supernatants of rat preoptic area (POA) primary cultures under the influence of Cygb. POA primary cultures cultured on poly-L-lysine-coated glass coverslips, were incubated for 240 min with fresh medium containing PBS (negative control), LPS at the concentration of 10 μg/ml (positive control) or LPS (10 μg/ml) plus Cygb (10 μg/ml or 20 μg/ml). LPS caused a significant increase in TNF-α and IL-6 concentrations in the supernatants of POA primary cultures and the co-treatment with Cygb prevent significantly this increase at the dose 20 μg/ml for TNF-α (A) and IL-6 (B) . The viability of the cells is not altered in any tested group (C) . Columns (means of 3–4 samples from three to six independent experiments) represent means with SEM (significant difference vs. LPS control group; * p

    Article Snippet: To stimulate the cells, they were incubated with LPS (Sigma–Aldrich, St. Louis, MO, USA; 10 μg/ml) or recombinant rat Cygb (Cusabio Technology, Houston, TX, USA; 10 and 20 μg/ml) for 4 h. The concentrations of LPS and Cygb used were based on previous studies (Wen et al., ; Leisengang et al., ).

    Techniques: Cell Culture, Incubation, Negative Control, Concentration Assay, Positive Control

    Cygb does not affect the nuclear NF-IL6 and STAT3 immunoreactivity in microglia and astrocytes, respectively. Immunocytochemistry was proceeded in coverslips using the NF-IL6 antiserum in ED1-positive microglia (A,C) and STAT3 antiserum in GFAP-positive astrocytes (B,D) . The immunoreactivity was enhanced in the cells treated with LPS (10 μg/ml) compared to the PBS group and the co-treatment with Cygb (10 μg/ml or 20 μg/ml) does not affect this. In panel (C) , triple labeling for ED-1 (in green), NF-IL-6 (in red) and cellular nuclei by DAPI (in blue) allowed localization of NF-IL6 immunoreactivity in the nuclei of microglial cells. In panel (D) , triple labeling for GFAP (in green), STAT3 (in red) and cellular nuclei by DAPI (in blue) allowed localization of STAT3 immunoreactivity in the nuclei of astrocytes. Secondary antisera employed were coupled to fluorophores Alexa-488 (green label) and Cy3 (red label). Scale bars: 20 μm. The average intensities of the signals within the active region of interest (here: cell nuclei) were expressed as gray values. Columns represent means of the intensities measured in treated cultures derived from two independent preparations (*** p

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Cytoglobin Attenuates Neuroinflammation in Lipopolysaccharide-Activated Primary Preoptic Area Cells via NF-κB Pathway Inhibition

    doi: 10.3389/fnmol.2019.00307

    Figure Lengend Snippet: Cygb does not affect the nuclear NF-IL6 and STAT3 immunoreactivity in microglia and astrocytes, respectively. Immunocytochemistry was proceeded in coverslips using the NF-IL6 antiserum in ED1-positive microglia (A,C) and STAT3 antiserum in GFAP-positive astrocytes (B,D) . The immunoreactivity was enhanced in the cells treated with LPS (10 μg/ml) compared to the PBS group and the co-treatment with Cygb (10 μg/ml or 20 μg/ml) does not affect this. In panel (C) , triple labeling for ED-1 (in green), NF-IL-6 (in red) and cellular nuclei by DAPI (in blue) allowed localization of NF-IL6 immunoreactivity in the nuclei of microglial cells. In panel (D) , triple labeling for GFAP (in green), STAT3 (in red) and cellular nuclei by DAPI (in blue) allowed localization of STAT3 immunoreactivity in the nuclei of astrocytes. Secondary antisera employed were coupled to fluorophores Alexa-488 (green label) and Cy3 (red label). Scale bars: 20 μm. The average intensities of the signals within the active region of interest (here: cell nuclei) were expressed as gray values. Columns represent means of the intensities measured in treated cultures derived from two independent preparations (*** p

    Article Snippet: To stimulate the cells, they were incubated with LPS (Sigma–Aldrich, St. Louis, MO, USA; 10 μg/ml) or recombinant rat Cygb (Cusabio Technology, Houston, TX, USA; 10 and 20 μg/ml) for 4 h. The concentrations of LPS and Cygb used were based on previous studies (Wen et al., ; Leisengang et al., ).

    Techniques: Immunocytochemistry, Labeling, Derivative Assay

    Nuclear factor-κB (NF-κB) immunoreactivity in microglia in the primary microculture of rat POA in the response of stimulation of LPS and treatment with Cygb. NF-κB immunoreactivity was detected in ED1-positive microglia after 240 min stimulation of primary cultures with medium containing 10 μg/ml LPS (C) , while the immunoreactivity in the PBS (A) groups was discrete. Triple labeling for ED-1 (in green), NF-κB (in red) and cellular nuclei by DAPI (in blue) demonstrate localization of NF-κB mainly to the nucleus of LPS-treated ED1-positive cells (C) , while there was a decrease NF-κB immunoreactivity in the cells treated with Cygb (20 μg/ml; E) . There are no significant changes in immunoreactivity in PBS + Cygb 20 μg or LPS + Cygb 10 μg groups ( B,D , respectively). Secondary antisera employed were coupled to fluorophores Alexa-488 (green label) and Cy3 (red label). Scale bars: 20 μm. The average intensities of the signals within the active region of interest (here: cell nuclei) were expressed as gray values (F) . Columns represent means of the intensities measured in treated cultures derived from two independent preparations (** p

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Cytoglobin Attenuates Neuroinflammation in Lipopolysaccharide-Activated Primary Preoptic Area Cells via NF-κB Pathway Inhibition

    doi: 10.3389/fnmol.2019.00307

    Figure Lengend Snippet: Nuclear factor-κB (NF-κB) immunoreactivity in microglia in the primary microculture of rat POA in the response of stimulation of LPS and treatment with Cygb. NF-κB immunoreactivity was detected in ED1-positive microglia after 240 min stimulation of primary cultures with medium containing 10 μg/ml LPS (C) , while the immunoreactivity in the PBS (A) groups was discrete. Triple labeling for ED-1 (in green), NF-κB (in red) and cellular nuclei by DAPI (in blue) demonstrate localization of NF-κB mainly to the nucleus of LPS-treated ED1-positive cells (C) , while there was a decrease NF-κB immunoreactivity in the cells treated with Cygb (20 μg/ml; E) . There are no significant changes in immunoreactivity in PBS + Cygb 20 μg or LPS + Cygb 10 μg groups ( B,D , respectively). Secondary antisera employed were coupled to fluorophores Alexa-488 (green label) and Cy3 (red label). Scale bars: 20 μm. The average intensities of the signals within the active region of interest (here: cell nuclei) were expressed as gray values (F) . Columns represent means of the intensities measured in treated cultures derived from two independent preparations (** p

    Article Snippet: To stimulate the cells, they were incubated with LPS (Sigma–Aldrich, St. Louis, MO, USA; 10 μg/ml) or recombinant rat Cygb (Cusabio Technology, Houston, TX, USA; 10 and 20 μg/ml) for 4 h. The concentrations of LPS and Cygb used were based on previous studies (Wen et al., ; Leisengang et al., ).

    Techniques: Labeling, Derivative Assay