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    Name:
    Rec Protein A
    Description:
    This recombinant protein A from Staphylococcus aureus Cowan I was expressed in Bacillus The gene has been altered to truncate the protein A at the carboxyl terminal This does not affect the binding characteristics
    Catalog Number:
    101100
    Price:
    None
    Applications:
    Immunoprecipitation|Protein Assays and Analysis|Protein Biology|Protein Purification & Isolation
    Category:
    Proteins Enzymes Peptides
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    Structured Review

    Thermo Fisher recombinant proteins
    This recombinant protein A from Staphylococcus aureus Cowan I was expressed in Bacillus The gene has been altered to truncate the protein A at the carboxyl terminal This does not affect the binding characteristics
    https://www.bioz.com/result/recombinant proteins/product/Thermo Fisher
    Average 99 stars, based on 1188 article reviews
    Price from $9.99 to $1999.99
    recombinant proteins - by Bioz Stars, 2020-11
    99/100 stars

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    Related Articles

    Electroporation:

    Article Title: Expression of recombinant Rhipicephalus (Boophilus) microplus, R. annulatus and R. decoloratus Bm86 orthologs as secreted proteins in Pichia pastoris
    Article Snippet: .. Pichia pastoris transformation and screening for recombinant protein expression Expression plasmids were linearized by restriction with Sac I and transformed into P. pastoris strains GS115, KM71H and X33 (Invitrogen) by electroporation as described [ ]. .. Transformants were selected on YPDS plates containing 100 μg·ml-1 Zeocin and incubated at 30°C.

    Transfection:

    Article Title: Characterization of an Entamoeba histolytica High-Mobility-Group Box Protein Induced during Intestinal Infection ▿ High-Mobility-Group Box Protein Induced during Intestinal Infection ▿ †
    Article Snippet: .. Gene expression in E. histolytica trophozoites overexpressing recombinant EhHMGB1 was compared to that of amebae transfected with an empty vector by Affymetrix gene chips. .. EhHMGB1 was found to be expressed at least 100-fold over the basal level in these transfected trophozoites.

    Article Title: LOC689986, a unique gene showing specific expression in restricted areas of the rodent neocortex
    Article Snippet: .. Detection of recombinant protein was achieved either directly (transient transfection using vectors encoding either a C- or N-terminal YFP tagged recombinant protein), or by using mouse anti-V5 primary antibody (diluted 1:1000, Invitrogen) and Alexa Fluor® 594 goat anti-mouse IgG (diluted 1:1000, Invitrogen) secondary antibody. .. Nuclei were stained with DAPI.

    Fluorescence:

    Article Title: Construction of a RFP-lacZα bicistronic reporter system and its application in lead biosensing
    Article Snippet: .. mCherry assay The fluorescent signal of mCherry produced in recombinant E . coli was measured with the Lumina fluorescence spectrometer (Thermo, USA) as previously described [ ]. .. Fluorescence emission was recorded at 610 nm for mCherry, and the fluorescence value was normalized by dividing the fluorescence intensity by the OD600 value of the same sample.

    Conjugation Assay:

    Article Title: Generation of Monoclonal Antibodies against Immunoglobulin Proteins of the Domestic Ferret (Mustela putorius furo)
    Article Snippet: .. Protein Conjugation Protein A/G purified mAb or recombinant Protein L were conjugated to DyLight 488 (ThermoFisher, Cat #46402), DyLight 650 (ThermoFisher, Cat #62265), or EZ-Link™ NHS-LC-Biotin (ThermoFisher, Cat #21336) according to the manufacture's instructions. ..

    Cell Culture:

    Article Title: Transduction of E2F-1 TAT fusion proteins represses expression of hTERT in primary ductal breast carcinoma cell lines
    Article Snippet: .. Dialysis of recombinant proteins into cell culture media E2F-1/TatHA and E132/TatHA recombinant proteins were diluted with Buffer B2 to a concentration of 0.5 mg/ml to avoid protein precipitation cascades and injected into 12 ml Slide-A-Lyzer® Dialysis Cassettes (Pierce Biotechnology, Inc., Rockford, IL) using 21-gauge, 1-inch beveled hypodermic needles. ..

    Purification:

    Article Title: Dissecting the human serum antibody response to secondary dengue virus infections
    Article Snippet: .. Recombinant E protein depletion of DENV-specific antibodies from immune sera Purified DENV2 rE protein was conjugated to magnetic dynabeads M-270 Epoxy (Invitrogen by Life Technologies) with a bead (mg) to ligand (ug) ratio of 5:1. .. Beads were washed three times with 0.1M Sodium Phosphate (7.4) followed by an overnight incubation with equal volumes of purified rE protein, 0.1M Borate buffer (pH 9.5) and 3M Ammonium sulfate at 37°C.

    Article Title: Molecular basis of lutropin recognition by the mannose/GalNAc-4-SO4 receptor
    Article Snippet: .. Purified recombinant protein A (Pierce) at 100 μg/ml in 10 mM sodium acetate buffer, pH 4.5 was coupled to a BIAcore CM5 sensorchip by using the Amine Coupling Kit provided by the manufacturer. ..

    Article Title: Generation of Monoclonal Antibodies against Immunoglobulin Proteins of the Domestic Ferret (Mustela putorius furo)
    Article Snippet: .. Protein Conjugation Protein A/G purified mAb or recombinant Protein L were conjugated to DyLight 488 (ThermoFisher, Cat #46402), DyLight 650 (ThermoFisher, Cat #62265), or EZ-Link™ NHS-LC-Biotin (ThermoFisher, Cat #21336) according to the manufacture's instructions. ..

    Produced:

    Article Title: Construction of a RFP-lacZα bicistronic reporter system and its application in lead biosensing
    Article Snippet: .. mCherry assay The fluorescent signal of mCherry produced in recombinant E . coli was measured with the Lumina fluorescence spectrometer (Thermo, USA) as previously described [ ]. .. Fluorescence emission was recorded at 610 nm for mCherry, and the fluorescence value was normalized by dividing the fluorescence intensity by the OD600 value of the same sample.

    Concentration Assay:

    Article Title: Transduction of E2F-1 TAT fusion proteins represses expression of hTERT in primary ductal breast carcinoma cell lines
    Article Snippet: .. Dialysis of recombinant proteins into cell culture media E2F-1/TatHA and E132/TatHA recombinant proteins were diluted with Buffer B2 to a concentration of 0.5 mg/ml to avoid protein precipitation cascades and injected into 12 ml Slide-A-Lyzer® Dialysis Cassettes (Pierce Biotechnology, Inc., Rockford, IL) using 21-gauge, 1-inch beveled hypodermic needles. ..

    Expressing:

    Article Title: Expression of recombinant Rhipicephalus (Boophilus) microplus, R. annulatus and R. decoloratus Bm86 orthologs as secreted proteins in Pichia pastoris
    Article Snippet: .. Pichia pastoris transformation and screening for recombinant protein expression Expression plasmids were linearized by restriction with Sac I and transformed into P. pastoris strains GS115, KM71H and X33 (Invitrogen) by electroporation as described [ ]. .. Transformants were selected on YPDS plates containing 100 μg·ml-1 Zeocin and incubated at 30°C.

    Article Title: Characterization of an Entamoeba histolytica High-Mobility-Group Box Protein Induced during Intestinal Infection ▿ High-Mobility-Group Box Protein Induced during Intestinal Infection ▿ †
    Article Snippet: .. Gene expression in E. histolytica trophozoites overexpressing recombinant EhHMGB1 was compared to that of amebae transfected with an empty vector by Affymetrix gene chips. .. EhHMGB1 was found to be expressed at least 100-fold over the basal level in these transfected trophozoites.

    Injection:

    Article Title: Transduction of E2F-1 TAT fusion proteins represses expression of hTERT in primary ductal breast carcinoma cell lines
    Article Snippet: .. Dialysis of recombinant proteins into cell culture media E2F-1/TatHA and E132/TatHA recombinant proteins were diluted with Buffer B2 to a concentration of 0.5 mg/ml to avoid protein precipitation cascades and injected into 12 ml Slide-A-Lyzer® Dialysis Cassettes (Pierce Biotechnology, Inc., Rockford, IL) using 21-gauge, 1-inch beveled hypodermic needles. ..

    Recombinant:

    Article Title: Transduction of E2F-1 TAT fusion proteins represses expression of hTERT in primary ductal breast carcinoma cell lines
    Article Snippet: .. Dialysis of recombinant proteins into cell culture media E2F-1/TatHA and E132/TatHA recombinant proteins were diluted with Buffer B2 to a concentration of 0.5 mg/ml to avoid protein precipitation cascades and injected into 12 ml Slide-A-Lyzer® Dialysis Cassettes (Pierce Biotechnology, Inc., Rockford, IL) using 21-gauge, 1-inch beveled hypodermic needles. ..

    Article Title: Dissecting the human serum antibody response to secondary dengue virus infections
    Article Snippet: .. Recombinant E protein depletion of DENV-specific antibodies from immune sera Purified DENV2 rE protein was conjugated to magnetic dynabeads M-270 Epoxy (Invitrogen by Life Technologies) with a bead (mg) to ligand (ug) ratio of 5:1. .. Beads were washed three times with 0.1M Sodium Phosphate (7.4) followed by an overnight incubation with equal volumes of purified rE protein, 0.1M Borate buffer (pH 9.5) and 3M Ammonium sulfate at 37°C.

    Article Title: Construction of a RFP-lacZα bicistronic reporter system and its application in lead biosensing
    Article Snippet: .. mCherry assay The fluorescent signal of mCherry produced in recombinant E . coli was measured with the Lumina fluorescence spectrometer (Thermo, USA) as previously described [ ]. .. Fluorescence emission was recorded at 610 nm for mCherry, and the fluorescence value was normalized by dividing the fluorescence intensity by the OD600 value of the same sample.

    Article Title: Molecular basis of lutropin recognition by the mannose/GalNAc-4-SO4 receptor
    Article Snippet: .. Purified recombinant protein A (Pierce) at 100 μg/ml in 10 mM sodium acetate buffer, pH 4.5 was coupled to a BIAcore CM5 sensorchip by using the Amine Coupling Kit provided by the manufacturer. ..

    Article Title: Expression of recombinant Rhipicephalus (Boophilus) microplus, R. annulatus and R. decoloratus Bm86 orthologs as secreted proteins in Pichia pastoris
    Article Snippet: .. Pichia pastoris transformation and screening for recombinant protein expression Expression plasmids were linearized by restriction with Sac I and transformed into P. pastoris strains GS115, KM71H and X33 (Invitrogen) by electroporation as described [ ]. .. Transformants were selected on YPDS plates containing 100 μg·ml-1 Zeocin and incubated at 30°C.

    Article Title: Generation of Monoclonal Antibodies against Immunoglobulin Proteins of the Domestic Ferret (Mustela putorius furo)
    Article Snippet: .. Protein Conjugation Protein A/G purified mAb or recombinant Protein L were conjugated to DyLight 488 (ThermoFisher, Cat #46402), DyLight 650 (ThermoFisher, Cat #62265), or EZ-Link™ NHS-LC-Biotin (ThermoFisher, Cat #21336) according to the manufacture's instructions. ..

    Article Title: Characterization of an Entamoeba histolytica High-Mobility-Group Box Protein Induced during Intestinal Infection ▿ High-Mobility-Group Box Protein Induced during Intestinal Infection ▿ †
    Article Snippet: .. Gene expression in E. histolytica trophozoites overexpressing recombinant EhHMGB1 was compared to that of amebae transfected with an empty vector by Affymetrix gene chips. .. EhHMGB1 was found to be expressed at least 100-fold over the basal level in these transfected trophozoites.

    Article Title: LOC689986, a unique gene showing specific expression in restricted areas of the rodent neocortex
    Article Snippet: .. Detection of recombinant protein was achieved either directly (transient transfection using vectors encoding either a C- or N-terminal YFP tagged recombinant protein), or by using mouse anti-V5 primary antibody (diluted 1:1000, Invitrogen) and Alexa Fluor® 594 goat anti-mouse IgG (diluted 1:1000, Invitrogen) secondary antibody. .. Nuclei were stained with DAPI.

    Transformation Assay:

    Article Title: Expression of recombinant Rhipicephalus (Boophilus) microplus, R. annulatus and R. decoloratus Bm86 orthologs as secreted proteins in Pichia pastoris
    Article Snippet: .. Pichia pastoris transformation and screening for recombinant protein expression Expression plasmids were linearized by restriction with Sac I and transformed into P. pastoris strains GS115, KM71H and X33 (Invitrogen) by electroporation as described [ ]. .. Transformants were selected on YPDS plates containing 100 μg·ml-1 Zeocin and incubated at 30°C.

    Plasmid Preparation:

    Article Title: Characterization of an Entamoeba histolytica High-Mobility-Group Box Protein Induced during Intestinal Infection ▿ High-Mobility-Group Box Protein Induced during Intestinal Infection ▿ †
    Article Snippet: .. Gene expression in E. histolytica trophozoites overexpressing recombinant EhHMGB1 was compared to that of amebae transfected with an empty vector by Affymetrix gene chips. .. EhHMGB1 was found to be expressed at least 100-fold over the basal level in these transfected trophozoites.

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  • 99
    Thermo Fisher c elegans protein sequence databases
    Human FAM173B is an evolutionarily conserved mitochondrial protein. A , phylogram of FAM173-like proteins. The tree encompasses FAM173A ( blue ) and FAM173B ( red ) proteins from the vertebrates Homo sapiens ( Hs ), Mus musculus ( Mm ), Xenopus laevis ( Xl ), Salmo salar ( Ss ), Danio rerio ( Dr ), and Gallus gallus ( Gg ), as well as FAM173-like proteins from the nonvertebrate animals ( green ) Capitella teleta ( Cte ), C. <t>elegans</t> ( Cel ), Tribolium castaneum ( Tca ), Daphnia pulex ( Dpu ), Apis mellifera ( Ame ), Drosophila melanogaster ( Dme ), Aedes aegypti ( Aae ), and Hydra vulgaris ( Hvu ). Also included are various crenarchaeal homologues ( purple ), including the previously characterized aKMT enzyme from Sulfolobus islandicus ( Sis ) and corresponding homologues from Pyrolobus fumarii ( Pfu ), Hyperthermus butylicus ( Hbu ), Ignicoccus islandicus ( Iis ), Staphylothermus hellenicus ( She ), and Methanobacterium formicicum ( Mfo ), as well as the bacterial aKMT-like enzymes ( brown ) from Pseudomonas stutzeri ( Pst ), Salinibacter ruber ( Sru ), Rhodopseudomonas palustris ( Rpa ), and Agrobacterium (multispecies) ( Agr . B , sequence elements of the N-terminal portion of FAM173B and alignment of the N-terminal part of putative FAM173 orthologues from organisms denoted as in A. Colors indicate the position of nonconserved NTS ( gray ), predicted TMD ( yellow ), conserved preMT ( orange ), and N-terminal fragment of the MTase domain ( green ). Hallmark motifs of the 7BS MTase domain are indicated by black boxes . The position of the conserved acidic residue in motif Post I (Glu-117 in H. sapiens FAM173B), crucial for AdoMet binding, is marked with an asterisk. C , subcellular localization of FAM173B-derived GFP fusion proteins. A schematic representation of the used FAM173B-derived sequences is given ( top ), with the various domains denoted as in B . Shown are confocal fluorescence microscopy images of HeLa cells, fixed 24 h after transient transfection with plasmids encoding FAM173B-GFP, (Δ55)-FAM173B-GFP, or (56–90)-FAM173B-GFP. Cells were counterstained with anti-COX IV primary antibody, followed by Alexa Fluor 568–conjugated secondary antibody, to visualize the mitochondria, and with DAPI, to visualize the nuclei. Data were acquired through green (GFP), red (COX IV), and blue (DAPI) channels and merged.
    C Elegans Protein Sequence Databases, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c elegans protein sequence databases/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    c elegans protein sequence databases - by Bioz Stars, 2020-11
    99/100 stars
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    90
    Thermo Fisher standard recombinant proteins cox2
    A significant amount of <t>COX2</t> protein is present in human platelets (A) Both COX2 and COX1 proteins were detected in platelets by Western blot analysis on platelet lysates along with recombinant COX2 and COX1 proteins (rCOX2, rCOX1). (B) Estimate of COX1 protein level in 10 μg of platelet lysate by Western-blotting using a series of rCOX1 standards. (C) Estimate of COX2 protein level in 10 μg of platelet lysate by Western-blotting using a series of rCOX2 standards. (D) Comparison (by Western blot) of COX2 and COX1 protein levels in ovarian cancer cells and platelets, platelet microparticles (MP). MP(I): MP prepared by using calcium inophore as the agonist. MO(Col): MP prepared by using collagen and thrombin as the agonists. (E) Colocalization of COX2 and platelet marker CD42b as demonstrated by double immunofluorescence staining.
    Standard Recombinant Proteins Cox2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/standard recombinant proteins cox2/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    standard recombinant proteins cox2 - by Bioz Stars, 2020-11
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    94
    Thermo Fisher western blot purified recombinant proteins
    Heterospecific and homospecific antibodies to TRR1. (A) Titers of homospecific and heterospecific antibodies in animals immunized with TRR1. Each animal was immunized three times with TRR1 from a single species or with the protein from all three species, one at a time. Two months after the last injection their sera were collected and used in ELISA experiments in which the plates were coated with TRR1 from each of the three fungal species. Bars represent the titers of antibodies (IgA + IgG + IgM) against each one of those species. (B) Sera from mice that were immunized with all three fungal species in sequence were used in <t>western</t> <t>blot</t> experiments to detect binding to <t>recombinant</t> C. albicans (lane b ), C. neoformans (lanes a , d ), and P. lutzii (lane e ) TRR1 <t>proteins,</t> as well as the protein extract of the human cell line HEK293 (lanes c , f ).
    Western Blot Purified Recombinant Proteins, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/western blot purified recombinant proteins/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    92
    Thermo Fisher recombinant proteins dsg1
    SpeB is a bacterial determinant for cleavage of desmogleins. (A) Culture supernatant from S. pyogenes strain 591 was pretreated with various types of protease inhibitors at room temperature for 30 min, then incubated with <t>Dsg1</t> or Dsg3 recombinant protein at 37°C for 3 h. (B) Recombinant desmogleins were separately treated with culture supernatants from S. pyogenes strains at 37°C for 3 h. An in-frame speB deletion mutant and its revertant strain with a background of NIH35 or 591 were employed for analysis. (C) Dsg1 and Dsg3 recombinant proteins were separately incubated with various concentrations of recombinant SpeB at 37°C for 3 h, then cleavage of desmogleins was detected by western blot analysis. White and black arrowheads indicate the full-length band and cleavage fragment, respectively.
    Recombinant Proteins Dsg1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant proteins dsg1/product/Thermo Fisher
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    recombinant proteins dsg1 - by Bioz Stars, 2020-11
    92/100 stars
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    Image Search Results


    Human FAM173B is an evolutionarily conserved mitochondrial protein. A , phylogram of FAM173-like proteins. The tree encompasses FAM173A ( blue ) and FAM173B ( red ) proteins from the vertebrates Homo sapiens ( Hs ), Mus musculus ( Mm ), Xenopus laevis ( Xl ), Salmo salar ( Ss ), Danio rerio ( Dr ), and Gallus gallus ( Gg ), as well as FAM173-like proteins from the nonvertebrate animals ( green ) Capitella teleta ( Cte ), C. elegans ( Cel ), Tribolium castaneum ( Tca ), Daphnia pulex ( Dpu ), Apis mellifera ( Ame ), Drosophila melanogaster ( Dme ), Aedes aegypti ( Aae ), and Hydra vulgaris ( Hvu ). Also included are various crenarchaeal homologues ( purple ), including the previously characterized aKMT enzyme from Sulfolobus islandicus ( Sis ) and corresponding homologues from Pyrolobus fumarii ( Pfu ), Hyperthermus butylicus ( Hbu ), Ignicoccus islandicus ( Iis ), Staphylothermus hellenicus ( She ), and Methanobacterium formicicum ( Mfo ), as well as the bacterial aKMT-like enzymes ( brown ) from Pseudomonas stutzeri ( Pst ), Salinibacter ruber ( Sru ), Rhodopseudomonas palustris ( Rpa ), and Agrobacterium (multispecies) ( Agr . B , sequence elements of the N-terminal portion of FAM173B and alignment of the N-terminal part of putative FAM173 orthologues from organisms denoted as in A. Colors indicate the position of nonconserved NTS ( gray ), predicted TMD ( yellow ), conserved preMT ( orange ), and N-terminal fragment of the MTase domain ( green ). Hallmark motifs of the 7BS MTase domain are indicated by black boxes . The position of the conserved acidic residue in motif Post I (Glu-117 in H. sapiens FAM173B), crucial for AdoMet binding, is marked with an asterisk. C , subcellular localization of FAM173B-derived GFP fusion proteins. A schematic representation of the used FAM173B-derived sequences is given ( top ), with the various domains denoted as in B . Shown are confocal fluorescence microscopy images of HeLa cells, fixed 24 h after transient transfection with plasmids encoding FAM173B-GFP, (Δ55)-FAM173B-GFP, or (56–90)-FAM173B-GFP. Cells were counterstained with anti-COX IV primary antibody, followed by Alexa Fluor 568–conjugated secondary antibody, to visualize the mitochondria, and with DAPI, to visualize the nuclei. Data were acquired through green (GFP), red (COX IV), and blue (DAPI) channels and merged.

    Journal: The Journal of Biological Chemistry

    Article Title: Lysine methylation by the mitochondrial methyltransferase FAM173B optimizes the function of mitochondrial ATP synthase

    doi: 10.1074/jbc.RA118.005473

    Figure Lengend Snippet: Human FAM173B is an evolutionarily conserved mitochondrial protein. A , phylogram of FAM173-like proteins. The tree encompasses FAM173A ( blue ) and FAM173B ( red ) proteins from the vertebrates Homo sapiens ( Hs ), Mus musculus ( Mm ), Xenopus laevis ( Xl ), Salmo salar ( Ss ), Danio rerio ( Dr ), and Gallus gallus ( Gg ), as well as FAM173-like proteins from the nonvertebrate animals ( green ) Capitella teleta ( Cte ), C. elegans ( Cel ), Tribolium castaneum ( Tca ), Daphnia pulex ( Dpu ), Apis mellifera ( Ame ), Drosophila melanogaster ( Dme ), Aedes aegypti ( Aae ), and Hydra vulgaris ( Hvu ). Also included are various crenarchaeal homologues ( purple ), including the previously characterized aKMT enzyme from Sulfolobus islandicus ( Sis ) and corresponding homologues from Pyrolobus fumarii ( Pfu ), Hyperthermus butylicus ( Hbu ), Ignicoccus islandicus ( Iis ), Staphylothermus hellenicus ( She ), and Methanobacterium formicicum ( Mfo ), as well as the bacterial aKMT-like enzymes ( brown ) from Pseudomonas stutzeri ( Pst ), Salinibacter ruber ( Sru ), Rhodopseudomonas palustris ( Rpa ), and Agrobacterium (multispecies) ( Agr . B , sequence elements of the N-terminal portion of FAM173B and alignment of the N-terminal part of putative FAM173 orthologues from organisms denoted as in A. Colors indicate the position of nonconserved NTS ( gray ), predicted TMD ( yellow ), conserved preMT ( orange ), and N-terminal fragment of the MTase domain ( green ). Hallmark motifs of the 7BS MTase domain are indicated by black boxes . The position of the conserved acidic residue in motif Post I (Glu-117 in H. sapiens FAM173B), crucial for AdoMet binding, is marked with an asterisk. C , subcellular localization of FAM173B-derived GFP fusion proteins. A schematic representation of the used FAM173B-derived sequences is given ( top ), with the various domains denoted as in B . Shown are confocal fluorescence microscopy images of HeLa cells, fixed 24 h after transient transfection with plasmids encoding FAM173B-GFP, (Δ55)-FAM173B-GFP, or (56–90)-FAM173B-GFP. Cells were counterstained with anti-COX IV primary antibody, followed by Alexa Fluor 568–conjugated secondary antibody, to visualize the mitochondria, and with DAPI, to visualize the nuclei. Data were acquired through green (GFP), red (COX IV), and blue (DAPI) channels and merged.

    Article Snippet: MS data were analyzed using in-house maintained human, rat, mouse, and C. elegans protein sequence databases using SEQUESTTM and Proteome DiscovererTM (Thermo Fisher Scientific).

    Techniques: Recombinase Polymerase Amplification, Sequencing, Binding Assay, Derivative Assay, Fluorescence, Microscopy, Transfection

    A significant amount of COX2 protein is present in human platelets (A) Both COX2 and COX1 proteins were detected in platelets by Western blot analysis on platelet lysates along with recombinant COX2 and COX1 proteins (rCOX2, rCOX1). (B) Estimate of COX1 protein level in 10 μg of platelet lysate by Western-blotting using a series of rCOX1 standards. (C) Estimate of COX2 protein level in 10 μg of platelet lysate by Western-blotting using a series of rCOX2 standards. (D) Comparison (by Western blot) of COX2 and COX1 protein levels in ovarian cancer cells and platelets, platelet microparticles (MP). MP(I): MP prepared by using calcium inophore as the agonist. MO(Col): MP prepared by using collagen and thrombin as the agonists. (E) Colocalization of COX2 and platelet marker CD42b as demonstrated by double immunofluorescence staining.

    Journal: Platelets

    Article Title: A small amount of cyclooxygenase 2 (COX2) is constitutively expressed in platelets

    doi: 10.1080/09537104.2016.1203406

    Figure Lengend Snippet: A significant amount of COX2 protein is present in human platelets (A) Both COX2 and COX1 proteins were detected in platelets by Western blot analysis on platelet lysates along with recombinant COX2 and COX1 proteins (rCOX2, rCOX1). (B) Estimate of COX1 protein level in 10 μg of platelet lysate by Western-blotting using a series of rCOX1 standards. (C) Estimate of COX2 protein level in 10 μg of platelet lysate by Western-blotting using a series of rCOX2 standards. (D) Comparison (by Western blot) of COX2 and COX1 protein levels in ovarian cancer cells and platelets, platelet microparticles (MP). MP(I): MP prepared by using calcium inophore as the agonist. MO(Col): MP prepared by using collagen and thrombin as the agonists. (E) Colocalization of COX2 and platelet marker CD42b as demonstrated by double immunofluorescence staining.

    Article Snippet: Standard recombinant proteins COX2 and COX1 were purchased from ThermoFisher Scientific and United States Biological, respectively.

    Techniques: Western Blot, Recombinant, Marker, Double Immunofluorescence Staining

    COX2 mRNA is detected in human platelets (A–B) Establishment of linear regressional equations for output variable Ct of RT-PCR and input variable logged cDNA copy number of COX2 and COX1, respectively. (C) Estimate of the COX2 mRNA level relative to that of COX1 in platelets and ovarian cell lines by RT-PCR. Total RNA samples from platelets and ovarian cell lines were assayed by RT-PCR to obtain corresponding Ct values. COX2 and COX1 mRNA copy numbers were calculated by using the above mentioned linear regressional equations and subsequent exponential transformation.

    Journal: Platelets

    Article Title: A small amount of cyclooxygenase 2 (COX2) is constitutively expressed in platelets

    doi: 10.1080/09537104.2016.1203406

    Figure Lengend Snippet: COX2 mRNA is detected in human platelets (A–B) Establishment of linear regressional equations for output variable Ct of RT-PCR and input variable logged cDNA copy number of COX2 and COX1, respectively. (C) Estimate of the COX2 mRNA level relative to that of COX1 in platelets and ovarian cell lines by RT-PCR. Total RNA samples from platelets and ovarian cell lines were assayed by RT-PCR to obtain corresponding Ct values. COX2 and COX1 mRNA copy numbers were calculated by using the above mentioned linear regressional equations and subsequent exponential transformation.

    Article Snippet: Standard recombinant proteins COX2 and COX1 were purchased from ThermoFisher Scientific and United States Biological, respectively.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Transformation Assay

    Heterospecific and homospecific antibodies to TRR1. (A) Titers of homospecific and heterospecific antibodies in animals immunized with TRR1. Each animal was immunized three times with TRR1 from a single species or with the protein from all three species, one at a time. Two months after the last injection their sera were collected and used in ELISA experiments in which the plates were coated with TRR1 from each of the three fungal species. Bars represent the titers of antibodies (IgA + IgG + IgM) against each one of those species. (B) Sera from mice that were immunized with all three fungal species in sequence were used in western blot experiments to detect binding to recombinant C. albicans (lane b ), C. neoformans (lanes a , d ), and P. lutzii (lane e ) TRR1 proteins, as well as the protein extract of the human cell line HEK293 (lanes c , f ).

    Journal: Frontiers in Microbiology

    Article Title: Thioredoxin Reductase 1 Is a Highly Immunogenic Cell Surface Antigen in Paracoccidioides spp., Candida albicans, and Cryptococcus neoformans

    doi: 10.3389/fmicb.2019.02930

    Figure Lengend Snippet: Heterospecific and homospecific antibodies to TRR1. (A) Titers of homospecific and heterospecific antibodies in animals immunized with TRR1. Each animal was immunized three times with TRR1 from a single species or with the protein from all three species, one at a time. Two months after the last injection their sera were collected and used in ELISA experiments in which the plates were coated with TRR1 from each of the three fungal species. Bars represent the titers of antibodies (IgA + IgG + IgM) against each one of those species. (B) Sera from mice that were immunized with all three fungal species in sequence were used in western blot experiments to detect binding to recombinant C. albicans (lane b ), C. neoformans (lanes a , d ), and P. lutzii (lane e ) TRR1 proteins, as well as the protein extract of the human cell line HEK293 (lanes c , f ).

    Article Snippet: Western Blot Purified recombinant proteins (144 ng per lane), HEK293 protein extracts (80 μg per lane) and a molecular weight marker (PageRuler Prestained Protein Ladder – Thermo Fisher) were separated by electrophoresis on denaturing 10% polyacrylamide gels.

    Techniques: Injection, Enzyme-linked Immunosorbent Assay, Mouse Assay, Sequencing, Western Blot, Binding Assay, Recombinant

    SpeB is a bacterial determinant for cleavage of desmogleins. (A) Culture supernatant from S. pyogenes strain 591 was pretreated with various types of protease inhibitors at room temperature for 30 min, then incubated with Dsg1 or Dsg3 recombinant protein at 37°C for 3 h. (B) Recombinant desmogleins were separately treated with culture supernatants from S. pyogenes strains at 37°C for 3 h. An in-frame speB deletion mutant and its revertant strain with a background of NIH35 or 591 were employed for analysis. (C) Dsg1 and Dsg3 recombinant proteins were separately incubated with various concentrations of recombinant SpeB at 37°C for 3 h, then cleavage of desmogleins was detected by western blot analysis. White and black arrowheads indicate the full-length band and cleavage fragment, respectively.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Streptococcal Cysteine Protease-Mediated Cleavage of Desmogleins Is Involved in the Pathogenesis of Cutaneous Infection

    doi: 10.3389/fcimb.2018.00010

    Figure Lengend Snippet: SpeB is a bacterial determinant for cleavage of desmogleins. (A) Culture supernatant from S. pyogenes strain 591 was pretreated with various types of protease inhibitors at room temperature for 30 min, then incubated with Dsg1 or Dsg3 recombinant protein at 37°C for 3 h. (B) Recombinant desmogleins were separately treated with culture supernatants from S. pyogenes strains at 37°C for 3 h. An in-frame speB deletion mutant and its revertant strain with a background of NIH35 or 591 were employed for analysis. (C) Dsg1 and Dsg3 recombinant proteins were separately incubated with various concentrations of recombinant SpeB at 37°C for 3 h, then cleavage of desmogleins was detected by western blot analysis. White and black arrowheads indicate the full-length band and cleavage fragment, respectively.

    Article Snippet: For construction of the recombinant proteins Dsg1 and Dsg3, cDNA from HaCaT cells was prepared using Trizol and a PureLink RNA mini-kit (Thermo Fisher Scientific).

    Techniques: Incubation, Recombinant, Mutagenesis, Western Blot

    S. pyogenes culture supernatants induce cleavage of Dsg1 and Dsg3. Recombinant extracellular domains of Dsg1 and Dsg3 were separately incubated with culture supernatants from S. pyogenes clinical isolates for 3 h at 37°C. Sample proteins were separated by SDS-PAGE under a reducing condition, and subjected to western blot analysis using specific antibodies against the extracellular domain of Dsg1 and Dsg3.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Streptococcal Cysteine Protease-Mediated Cleavage of Desmogleins Is Involved in the Pathogenesis of Cutaneous Infection

    doi: 10.3389/fcimb.2018.00010

    Figure Lengend Snippet: S. pyogenes culture supernatants induce cleavage of Dsg1 and Dsg3. Recombinant extracellular domains of Dsg1 and Dsg3 were separately incubated with culture supernatants from S. pyogenes clinical isolates for 3 h at 37°C. Sample proteins were separated by SDS-PAGE under a reducing condition, and subjected to western blot analysis using specific antibodies against the extracellular domain of Dsg1 and Dsg3.

    Article Snippet: For construction of the recombinant proteins Dsg1 and Dsg3, cDNA from HaCaT cells was prepared using Trizol and a PureLink RNA mini-kit (Thermo Fisher Scientific).

    Techniques: Recombinant, Incubation, SDS Page, Western Blot

    SpeB-mediated cleavage of desmogleins contributes to epidermal barrier dysfunction. Cutaneous sections obtained from mice infected with the examined S. pyogenes strains were subjected to immunofluorescence staining. Dsg1 and Dsg3 were labeled with anti-Dsg1 and anti-Dsg3 antibodies, respectively, followed by incubation with an Alexa Fluor 647-conjugated antibody. S. pyogenes was labeled with anti-Group A carbohydrate and Alexa Fluor 488-conjugated antibodies, and cell nuclei were stained with Hoechst 33342. Obtained tissue sections were analyzed using a confocal laser microscope. The boxed area is magnified in the box panel below. Data shown are representative of at least three separate experiments.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Streptococcal Cysteine Protease-Mediated Cleavage of Desmogleins Is Involved in the Pathogenesis of Cutaneous Infection

    doi: 10.3389/fcimb.2018.00010

    Figure Lengend Snippet: SpeB-mediated cleavage of desmogleins contributes to epidermal barrier dysfunction. Cutaneous sections obtained from mice infected with the examined S. pyogenes strains were subjected to immunofluorescence staining. Dsg1 and Dsg3 were labeled with anti-Dsg1 and anti-Dsg3 antibodies, respectively, followed by incubation with an Alexa Fluor 647-conjugated antibody. S. pyogenes was labeled with anti-Group A carbohydrate and Alexa Fluor 488-conjugated antibodies, and cell nuclei were stained with Hoechst 33342. Obtained tissue sections were analyzed using a confocal laser microscope. The boxed area is magnified in the box panel below. Data shown are representative of at least three separate experiments.

    Article Snippet: For construction of the recombinant proteins Dsg1 and Dsg3, cDNA from HaCaT cells was prepared using Trizol and a PureLink RNA mini-kit (Thermo Fisher Scientific).

    Techniques: Mouse Assay, Infection, Immunofluorescence, Staining, Labeling, Incubation, Microscopy