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    Name:
    Purification and Analysis
    Description:
    Covering vital and traditional topics in the purification and analysis of recombinant proteins this volume demonstrates how to overcome problems in protein research and presents practical methods used in protein work explaining their theoretical basis The collection also explores innovative concepts such as receptor affinity chromatography and the introduction of sites that facilitate purification This aid uses specific examples of proteins obtained from bacterial and nonbacterial sources and supplies helpful approaches and techniques
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    Structured Review

    Millipore recombinant proteins
    Purification and Analysis
    Covering vital and traditional topics in the purification and analysis of recombinant proteins this volume demonstrates how to overcome problems in protein research and presents practical methods used in protein work explaining their theoretical basis The collection also explores innovative concepts such as receptor affinity chromatography and the introduction of sites that facilitate purification This aid uses specific examples of proteins obtained from bacterial and nonbacterial sources and supplies helpful approaches and techniques
    https://www.bioz.com/result/recombinant proteins/product/Millipore
    Average 99 stars, based on 1379 article reviews
    Price from $9.99 to $1999.99
    recombinant proteins - by Bioz Stars, 2020-11
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: The Amino Acid Arginine 210 of the Response Regulator HrpG of Xanthomonas citri subsp. citri Is Required for HrpG Function in Virulence
    Article Snippet: .. Expression and purification of recombinant proteins hrpG and hrpG-R210C were amplified by PCR from pBBHrpG and pBBR210C, respectively, by using the oligonucleotides HrpGBamHIup and HrpGXhoIdown and cloned into pET32am vector that allows expression of fusion proteins to thioredoxin (Trx) and a 6X-His tag (Novagen), previously modified in our laboratory to eliminate S-tag portion and digested with the restriction enzymes BamHI and XhoI, leading to pET32-hrpG and pET32-hrpG-R210C plasmids. .. After transformation into E . coli BLR strain, protein synthesis was induced by 0.5 mM IPTG for 18 h at 18°C.

    Article Title: Ferritin Blocks Inhibitory Effects of Two-Chain High Molecular Weight Kininogen (HKa) on Adhesion and Survival Signaling in Endothelial Cells
    Article Snippet: .. Expression and purification of recombinant HFt and LFt cDNA encoding the human ferritin H subunit was cloned into the pET-17 bacterial expression vector (Novagen) and transformed into E.coli BL21(DE3) for expression. ..

    Amplification:

    Article Title: The Amino Acid Arginine 210 of the Response Regulator HrpG of Xanthomonas citri subsp. citri Is Required for HrpG Function in Virulence
    Article Snippet: .. Expression and purification of recombinant proteins hrpG and hrpG-R210C were amplified by PCR from pBBHrpG and pBBR210C, respectively, by using the oligonucleotides HrpGBamHIup and HrpGXhoIdown and cloned into pET32am vector that allows expression of fusion proteins to thioredoxin (Trx) and a 6X-His tag (Novagen), previously modified in our laboratory to eliminate S-tag portion and digested with the restriction enzymes BamHI and XhoI, leading to pET32-hrpG and pET32-hrpG-R210C plasmids. .. After transformation into E . coli BLR strain, protein synthesis was induced by 0.5 mM IPTG for 18 h at 18°C.

    Infection:

    Article Title: Novel chimeric virus-like particles vaccine displaying MERS-CoV receptor-binding domain induce specific humoral and cellular immune response in mice
    Article Snippet: .. 2.4 Western blotSamples of purified sVLP, purified recombinant RBD proteins, CPV VLP and lysate from pFastBac1 infected cells were transferred onto a polyvinylidene fluoride (PVDF) membrane (Immobilin-P, Millipore, USA) after SDS-PAGE for Western blotting with mouse anti-RBD or -VP2 monoclonal antibodies. .. 2.5 Characterization by electron and immunoelectron microscopysVLP were loaded onto grids, kept at room temperature for 5 min, stained with 1% sodium phosphotungstate, and then examined directly under a transmission electron microscope (TEM).

    Purification:

    Article Title: The Amino Acid Arginine 210 of the Response Regulator HrpG of Xanthomonas citri subsp. citri Is Required for HrpG Function in Virulence
    Article Snippet: .. Expression and purification of recombinant proteins hrpG and hrpG-R210C were amplified by PCR from pBBHrpG and pBBR210C, respectively, by using the oligonucleotides HrpGBamHIup and HrpGXhoIdown and cloned into pET32am vector that allows expression of fusion proteins to thioredoxin (Trx) and a 6X-His tag (Novagen), previously modified in our laboratory to eliminate S-tag portion and digested with the restriction enzymes BamHI and XhoI, leading to pET32-hrpG and pET32-hrpG-R210C plasmids. .. After transformation into E . coli BLR strain, protein synthesis was induced by 0.5 mM IPTG for 18 h at 18°C.

    Article Title: The Nucleocapsid Protein of Coronavirus Infectious Bronchitis Virus: Crystal Structure of Its N-Terminal Domain and Multimerization Properties
    Article Snippet: .. Crosslinking Experiments The purified recombinant proteins IBV-N, IBV-N29-160, and IBV-N218-329 were incubated with either glutaraldehyde or SAB (Sigma-Aldrich, St. Louis, MO) for 2 hr at 20°C using a constant amount of protein (5 μg) with increasing amounts of the crosslinking agent. .. The samples were submitted to electrophoresis on an 8%–15% SDS-PAGE gel and stained with Coomassie blue.

    Article Title: Ferritin Blocks Inhibitory Effects of Two-Chain High Molecular Weight Kininogen (HKa) on Adhesion and Survival Signaling in Endothelial Cells
    Article Snippet: .. Expression and purification of recombinant HFt and LFt cDNA encoding the human ferritin H subunit was cloned into the pET-17 bacterial expression vector (Novagen) and transformed into E.coli BL21(DE3) for expression. ..

    Article Title: Novel chimeric virus-like particles vaccine displaying MERS-CoV receptor-binding domain induce specific humoral and cellular immune response in mice
    Article Snippet: .. 2.4 Western blotSamples of purified sVLP, purified recombinant RBD proteins, CPV VLP and lysate from pFastBac1 infected cells were transferred onto a polyvinylidene fluoride (PVDF) membrane (Immobilin-P, Millipore, USA) after SDS-PAGE for Western blotting with mouse anti-RBD or -VP2 monoclonal antibodies. .. 2.5 Characterization by electron and immunoelectron microscopysVLP were loaded onto grids, kept at room temperature for 5 min, stained with 1% sodium phosphotungstate, and then examined directly under a transmission electron microscope (TEM).

    Article Title: Discovery of a small-molecule protein kinase Cδ-selective activator with promising application in colon cancer therapy
    Article Snippet: .. The non-radioactive PKC kinase activity kit (ADI-EKS-420A, Enzo Life Sciences) and purified recombinant human PKC proteins, cPKCs (mix of PKCα, β, and γ), PKCδ, PKCε, and PKCζ (Millipore, VWR, Carnaxide, Portugal), were used. .. Briefly, 10 ng PKC was incubated with PMA/ARA, Roy-Bz, or vehicle for 1 h, and then transferred to a 96-well plate pre-coated with a peptide pseudosubstrate.

    Protein Purification:

    Article Title: Identification of a Mycothiol-Dependent Nitroreductase from Mycobacterium tuberculosis
    Article Snippet: .. Plasmid Constructs and Protein Purification WT rv2466c was introduced into pET28B (Novagen), and the resulting plasmid was transformed into BL21(DE3) competent cells (Life technologies) with kanamycin selection (50 μg/mL). .. Bacteria were grown initially at 37 °C while shaking, and protein expression was induced using 0.75 mM IPTG at 16 °C for 18 h. Cells were pelleted and lysed using a sonicator and His tagged protein was purified with nickel chromatography and dialyzed into a 50 mM Tris, pH 7.5, 50 mM NaCl solution.

    Polymerase Chain Reaction:

    Article Title: The Amino Acid Arginine 210 of the Response Regulator HrpG of Xanthomonas citri subsp. citri Is Required for HrpG Function in Virulence
    Article Snippet: .. Expression and purification of recombinant proteins hrpG and hrpG-R210C were amplified by PCR from pBBHrpG and pBBR210C, respectively, by using the oligonucleotides HrpGBamHIup and HrpGXhoIdown and cloned into pET32am vector that allows expression of fusion proteins to thioredoxin (Trx) and a 6X-His tag (Novagen), previously modified in our laboratory to eliminate S-tag portion and digested with the restriction enzymes BamHI and XhoI, leading to pET32-hrpG and pET32-hrpG-R210C plasmids. .. After transformation into E . coli BLR strain, protein synthesis was induced by 0.5 mM IPTG for 18 h at 18°C.

    Incubation:

    Article Title: The Nucleocapsid Protein of Coronavirus Infectious Bronchitis Virus: Crystal Structure of Its N-Terminal Domain and Multimerization Properties
    Article Snippet: .. Crosslinking Experiments The purified recombinant proteins IBV-N, IBV-N29-160, and IBV-N218-329 were incubated with either glutaraldehyde or SAB (Sigma-Aldrich, St. Louis, MO) for 2 hr at 20°C using a constant amount of protein (5 μg) with increasing amounts of the crosslinking agent. .. The samples were submitted to electrophoresis on an 8%–15% SDS-PAGE gel and stained with Coomassie blue.

    Selection:

    Article Title: Identification of a Mycothiol-Dependent Nitroreductase from Mycobacterium tuberculosis
    Article Snippet: .. Plasmid Constructs and Protein Purification WT rv2466c was introduced into pET28B (Novagen), and the resulting plasmid was transformed into BL21(DE3) competent cells (Life technologies) with kanamycin selection (50 μg/mL). .. Bacteria were grown initially at 37 °C while shaking, and protein expression was induced using 0.75 mM IPTG at 16 °C for 18 h. Cells were pelleted and lysed using a sonicator and His tagged protein was purified with nickel chromatography and dialyzed into a 50 mM Tris, pH 7.5, 50 mM NaCl solution.

    Activity Assay:

    Article Title: Discovery of a small-molecule protein kinase Cδ-selective activator with promising application in colon cancer therapy
    Article Snippet: .. The non-radioactive PKC kinase activity kit (ADI-EKS-420A, Enzo Life Sciences) and purified recombinant human PKC proteins, cPKCs (mix of PKCα, β, and γ), PKCδ, PKCε, and PKCζ (Millipore, VWR, Carnaxide, Portugal), were used. .. Briefly, 10 ng PKC was incubated with PMA/ARA, Roy-Bz, or vehicle for 1 h, and then transferred to a 96-well plate pre-coated with a peptide pseudosubstrate.

    Construct:

    Article Title: Identification of a Mycothiol-Dependent Nitroreductase from Mycobacterium tuberculosis
    Article Snippet: .. Plasmid Constructs and Protein Purification WT rv2466c was introduced into pET28B (Novagen), and the resulting plasmid was transformed into BL21(DE3) competent cells (Life technologies) with kanamycin selection (50 μg/mL). .. Bacteria were grown initially at 37 °C while shaking, and protein expression was induced using 0.75 mM IPTG at 16 °C for 18 h. Cells were pelleted and lysed using a sonicator and His tagged protein was purified with nickel chromatography and dialyzed into a 50 mM Tris, pH 7.5, 50 mM NaCl solution.

    Expressing:

    Article Title: The Amino Acid Arginine 210 of the Response Regulator HrpG of Xanthomonas citri subsp. citri Is Required for HrpG Function in Virulence
    Article Snippet: .. Expression and purification of recombinant proteins hrpG and hrpG-R210C were amplified by PCR from pBBHrpG and pBBR210C, respectively, by using the oligonucleotides HrpGBamHIup and HrpGXhoIdown and cloned into pET32am vector that allows expression of fusion proteins to thioredoxin (Trx) and a 6X-His tag (Novagen), previously modified in our laboratory to eliminate S-tag portion and digested with the restriction enzymes BamHI and XhoI, leading to pET32-hrpG and pET32-hrpG-R210C plasmids. .. After transformation into E . coli BLR strain, protein synthesis was induced by 0.5 mM IPTG for 18 h at 18°C.

    Article Title: Ferritin Blocks Inhibitory Effects of Two-Chain High Molecular Weight Kininogen (HKa) on Adhesion and Survival Signaling in Endothelial Cells
    Article Snippet: .. Expression and purification of recombinant HFt and LFt cDNA encoding the human ferritin H subunit was cloned into the pET-17 bacterial expression vector (Novagen) and transformed into E.coli BL21(DE3) for expression. ..

    Modification:

    Article Title: The Amino Acid Arginine 210 of the Response Regulator HrpG of Xanthomonas citri subsp. citri Is Required for HrpG Function in Virulence
    Article Snippet: .. Expression and purification of recombinant proteins hrpG and hrpG-R210C were amplified by PCR from pBBHrpG and pBBR210C, respectively, by using the oligonucleotides HrpGBamHIup and HrpGXhoIdown and cloned into pET32am vector that allows expression of fusion proteins to thioredoxin (Trx) and a 6X-His tag (Novagen), previously modified in our laboratory to eliminate S-tag portion and digested with the restriction enzymes BamHI and XhoI, leading to pET32-hrpG and pET32-hrpG-R210C plasmids. .. After transformation into E . coli BLR strain, protein synthesis was induced by 0.5 mM IPTG for 18 h at 18°C.

    Western Blot:

    Article Title: Novel chimeric virus-like particles vaccine displaying MERS-CoV receptor-binding domain induce specific humoral and cellular immune response in mice
    Article Snippet: .. 2.4 Western blotSamples of purified sVLP, purified recombinant RBD proteins, CPV VLP and lysate from pFastBac1 infected cells were transferred onto a polyvinylidene fluoride (PVDF) membrane (Immobilin-P, Millipore, USA) after SDS-PAGE for Western blotting with mouse anti-RBD or -VP2 monoclonal antibodies. .. 2.5 Characterization by electron and immunoelectron microscopysVLP were loaded onto grids, kept at room temperature for 5 min, stained with 1% sodium phosphotungstate, and then examined directly under a transmission electron microscope (TEM).

    Transformation Assay:

    Article Title: Ferritin Blocks Inhibitory Effects of Two-Chain High Molecular Weight Kininogen (HKa) on Adhesion and Survival Signaling in Endothelial Cells
    Article Snippet: .. Expression and purification of recombinant HFt and LFt cDNA encoding the human ferritin H subunit was cloned into the pET-17 bacterial expression vector (Novagen) and transformed into E.coli BL21(DE3) for expression. ..

    Article Title: Identification of a Mycothiol-Dependent Nitroreductase from Mycobacterium tuberculosis
    Article Snippet: .. Plasmid Constructs and Protein Purification WT rv2466c was introduced into pET28B (Novagen), and the resulting plasmid was transformed into BL21(DE3) competent cells (Life technologies) with kanamycin selection (50 μg/mL). .. Bacteria were grown initially at 37 °C while shaking, and protein expression was induced using 0.75 mM IPTG at 16 °C for 18 h. Cells were pelleted and lysed using a sonicator and His tagged protein was purified with nickel chromatography and dialyzed into a 50 mM Tris, pH 7.5, 50 mM NaCl solution.

    Positron Emission Tomography:

    Article Title: Ferritin Blocks Inhibitory Effects of Two-Chain High Molecular Weight Kininogen (HKa) on Adhesion and Survival Signaling in Endothelial Cells
    Article Snippet: .. Expression and purification of recombinant HFt and LFt cDNA encoding the human ferritin H subunit was cloned into the pET-17 bacterial expression vector (Novagen) and transformed into E.coli BL21(DE3) for expression. ..

    Recombinant:

    Article Title: The Amino Acid Arginine 210 of the Response Regulator HrpG of Xanthomonas citri subsp. citri Is Required for HrpG Function in Virulence
    Article Snippet: .. Expression and purification of recombinant proteins hrpG and hrpG-R210C were amplified by PCR from pBBHrpG and pBBR210C, respectively, by using the oligonucleotides HrpGBamHIup and HrpGXhoIdown and cloned into pET32am vector that allows expression of fusion proteins to thioredoxin (Trx) and a 6X-His tag (Novagen), previously modified in our laboratory to eliminate S-tag portion and digested with the restriction enzymes BamHI and XhoI, leading to pET32-hrpG and pET32-hrpG-R210C plasmids. .. After transformation into E . coli BLR strain, protein synthesis was induced by 0.5 mM IPTG for 18 h at 18°C.

    Article Title: The Nucleocapsid Protein of Coronavirus Infectious Bronchitis Virus: Crystal Structure of Its N-Terminal Domain and Multimerization Properties
    Article Snippet: .. Crosslinking Experiments The purified recombinant proteins IBV-N, IBV-N29-160, and IBV-N218-329 were incubated with either glutaraldehyde or SAB (Sigma-Aldrich, St. Louis, MO) for 2 hr at 20°C using a constant amount of protein (5 μg) with increasing amounts of the crosslinking agent. .. The samples were submitted to electrophoresis on an 8%–15% SDS-PAGE gel and stained with Coomassie blue.

    Article Title: Ferritin Blocks Inhibitory Effects of Two-Chain High Molecular Weight Kininogen (HKa) on Adhesion and Survival Signaling in Endothelial Cells
    Article Snippet: .. Expression and purification of recombinant HFt and LFt cDNA encoding the human ferritin H subunit was cloned into the pET-17 bacterial expression vector (Novagen) and transformed into E.coli BL21(DE3) for expression. ..

    Article Title: Protective Efficacy of Coccidial Common Antigen Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) against Challenge with Three Eimeria Species
    Article Snippet: .. Rats were vaccinated subcutaneously in the back with 200 μg (400 μg/ml) recombinant proteins of EaGAPDH and EmGAPDH emulsified in 0.5 ml of Freund’s Adjuvant Complete (Sigma–Aldrich, Merck KGaA, Darmstadt, Germany) at 30 days of age separately. .. Two weeks later, two doses of proteins (200 μg, 400 μg/ml) emulsified in 0.5 ml of Freund’s Adjuvant Incomplete (Sigma–Aldrich) were given at an interval of 1 week.

    Article Title: Novel chimeric virus-like particles vaccine displaying MERS-CoV receptor-binding domain induce specific humoral and cellular immune response in mice
    Article Snippet: .. 2.4 Western blotSamples of purified sVLP, purified recombinant RBD proteins, CPV VLP and lysate from pFastBac1 infected cells were transferred onto a polyvinylidene fluoride (PVDF) membrane (Immobilin-P, Millipore, USA) after SDS-PAGE for Western blotting with mouse anti-RBD or -VP2 monoclonal antibodies. .. 2.5 Characterization by electron and immunoelectron microscopysVLP were loaded onto grids, kept at room temperature for 5 min, stained with 1% sodium phosphotungstate, and then examined directly under a transmission electron microscope (TEM).

    Article Title: Discovery of a small-molecule protein kinase Cδ-selective activator with promising application in colon cancer therapy
    Article Snippet: .. The non-radioactive PKC kinase activity kit (ADI-EKS-420A, Enzo Life Sciences) and purified recombinant human PKC proteins, cPKCs (mix of PKCα, β, and γ), PKCδ, PKCε, and PKCζ (Millipore, VWR, Carnaxide, Portugal), were used. .. Briefly, 10 ng PKC was incubated with PMA/ARA, Roy-Bz, or vehicle for 1 h, and then transferred to a 96-well plate pre-coated with a peptide pseudosubstrate.

    SDS Page:

    Article Title: Novel chimeric virus-like particles vaccine displaying MERS-CoV receptor-binding domain induce specific humoral and cellular immune response in mice
    Article Snippet: .. 2.4 Western blotSamples of purified sVLP, purified recombinant RBD proteins, CPV VLP and lysate from pFastBac1 infected cells were transferred onto a polyvinylidene fluoride (PVDF) membrane (Immobilin-P, Millipore, USA) after SDS-PAGE for Western blotting with mouse anti-RBD or -VP2 monoclonal antibodies. .. 2.5 Characterization by electron and immunoelectron microscopysVLP were loaded onto grids, kept at room temperature for 5 min, stained with 1% sodium phosphotungstate, and then examined directly under a transmission electron microscope (TEM).

    Plasmid Preparation:

    Article Title: The Amino Acid Arginine 210 of the Response Regulator HrpG of Xanthomonas citri subsp. citri Is Required for HrpG Function in Virulence
    Article Snippet: .. Expression and purification of recombinant proteins hrpG and hrpG-R210C were amplified by PCR from pBBHrpG and pBBR210C, respectively, by using the oligonucleotides HrpGBamHIup and HrpGXhoIdown and cloned into pET32am vector that allows expression of fusion proteins to thioredoxin (Trx) and a 6X-His tag (Novagen), previously modified in our laboratory to eliminate S-tag portion and digested with the restriction enzymes BamHI and XhoI, leading to pET32-hrpG and pET32-hrpG-R210C plasmids. .. After transformation into E . coli BLR strain, protein synthesis was induced by 0.5 mM IPTG for 18 h at 18°C.

    Article Title: Ferritin Blocks Inhibitory Effects of Two-Chain High Molecular Weight Kininogen (HKa) on Adhesion and Survival Signaling in Endothelial Cells
    Article Snippet: .. Expression and purification of recombinant HFt and LFt cDNA encoding the human ferritin H subunit was cloned into the pET-17 bacterial expression vector (Novagen) and transformed into E.coli BL21(DE3) for expression. ..

    Article Title: Identification of a Mycothiol-Dependent Nitroreductase from Mycobacterium tuberculosis
    Article Snippet: .. Plasmid Constructs and Protein Purification WT rv2466c was introduced into pET28B (Novagen), and the resulting plasmid was transformed into BL21(DE3) competent cells (Life technologies) with kanamycin selection (50 μg/mL). .. Bacteria were grown initially at 37 °C while shaking, and protein expression was induced using 0.75 mM IPTG at 16 °C for 18 h. Cells were pelleted and lysed using a sonicator and His tagged protein was purified with nickel chromatography and dialyzed into a 50 mM Tris, pH 7.5, 50 mM NaCl solution.

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  • 99
    Millipore inactive mek human recombinant proteins
    An illustration showing the modulation of the <t>BRAF/MEK/ERK/RSK2</t> signaling pathway by silybin in SK-MEL-5 cells
    Inactive Mek Human Recombinant Proteins, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/inactive mek human recombinant proteins/product/Millipore
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    inactive mek human recombinant proteins - by Bioz Stars, 2020-11
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      Buy from Supplier

    90
    Millipore rad51 protein
    Far western Immunoblotting of BLM termini. a Left panel Conventional western analysis of the BLM fragments using anti-FLAG M2 antibody. Right panel Far western analysis of BLM fragments interaction with <t>RAD51</t> using anti-RAD51 antibody. b Left panel Far
    Rad51 Protein, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rad51 protein/product/Millipore
    Average 90 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    rad51 protein - by Bioz Stars, 2020-11
    90/100 stars
      Buy from Supplier

    Image Search Results


    An illustration showing the modulation of the BRAF/MEK/ERK/RSK2 signaling pathway by silybin in SK-MEL-5 cells

    Journal: Cancer prevention research (Philadelphia, Pa.)

    Article Title: Direct targeting of MEK1/2 and RSK2 by silybin induces cell cycle arrest and inhibits melanoma cell growth

    doi: 10.1158/1940-6207.CAPR-12-0425

    Figure Lengend Snippet: An illustration showing the modulation of the BRAF/MEK/ERK/RSK2 signaling pathway by silybin in SK-MEL-5 cells

    Article Snippet: Active BRAF (V600E), active MEK1, inactive ERK2 (MEK substrate), active RSK2 and inactive MEK human recombinant proteins for kinase assays were purchased from Millipore (Temecula, CA).

    Techniques:

    Nanomotion experiments using a mixture of α -syn monomers and fibrils. Using a two-step procedure, the M17 cells undergo a rapid cytotoxic reaction and, after

    Journal: Cell Death Discovery

    Article Title: Amyloid single-cell cytotoxicity assays by nanomotion detection

    doi: 10.1038/cddiscovery.2017.53

    Figure Lengend Snippet: Nanomotion experiments using a mixture of α -syn monomers and fibrils. Using a two-step procedure, the M17 cells undergo a rapid cytotoxic reaction and, after

    Article Snippet: Preparation and characterization of α -syn recombinant proteins: preparation of crude mixture The α -syn crude mixture was generated incubating 800 μ l of a 45 μ M filtered (100 kDa filter) (Millipore) monomeric α -syn solution (50 mM Tris, 150 mM NaCl, pH 7.5) under constant orbital agitation (400 rpm) at 37 °C for 4 weeks.

    Techniques:

    Localization of α -syn species. Optical and fluorescence images of M17 cells on a cantilever sensor exposed for 2 h to OG-tagged α -syn monomeric ( a ) and fibrillar ( b ) forms. The fibrils are clearly localized on the cell surface while the monomers do not appear to localize on the cells. (The nanomotion response is shown in Supplementary Figure S6 ).

    Journal: Cell Death Discovery

    Article Title: Amyloid single-cell cytotoxicity assays by nanomotion detection

    doi: 10.1038/cddiscovery.2017.53

    Figure Lengend Snippet: Localization of α -syn species. Optical and fluorescence images of M17 cells on a cantilever sensor exposed for 2 h to OG-tagged α -syn monomeric ( a ) and fibrillar ( b ) forms. The fibrils are clearly localized on the cell surface while the monomers do not appear to localize on the cells. (The nanomotion response is shown in Supplementary Figure S6 ).

    Article Snippet: Preparation and characterization of α -syn recombinant proteins: preparation of crude mixture The α -syn crude mixture was generated incubating 800 μ l of a 45 μ M filtered (100 kDa filter) (Millipore) monomeric α -syn solution (50 mM Tris, 150 mM NaCl, pH 7.5) under constant orbital agitation (400 rpm) at 37 °C for 4 weeks.

    Techniques: Fluorescence

    Internalization of the Ethidium homodimer III. Optical and fluorescence images of M17 cells in ETH III-rich medium after 1, 3 and 4.5 h of incubation of cells with ( a ) α -syn monomers and ( b ) aggregated α -syn crude mixture at 0.12 μ M.

    Journal: Cell Death Discovery

    Article Title: Amyloid single-cell cytotoxicity assays by nanomotion detection

    doi: 10.1038/cddiscovery.2017.53

    Figure Lengend Snippet: Internalization of the Ethidium homodimer III. Optical and fluorescence images of M17 cells in ETH III-rich medium after 1, 3 and 4.5 h of incubation of cells with ( a ) α -syn monomers and ( b ) aggregated α -syn crude mixture at 0.12 μ M.

    Article Snippet: Preparation and characterization of α -syn recombinant proteins: preparation of crude mixture The α -syn crude mixture was generated incubating 800 μ l of a 45 μ M filtered (100 kDa filter) (Millipore) monomeric α -syn solution (50 mM Tris, 150 mM NaCl, pH 7.5) under constant orbital agitation (400 rpm) at 37 °C for 4 weeks.

    Techniques: Fluorescence, Incubation

    Nanomotion single-cell cytotoxicity studies. Typical nanomotion response of M17 neuroblastoma cells exposed to ( a ) monomeric α -syn and ( b ) the crude mixture of oligomers and fibrils. In both cases, the amyloid species were injected in a two-step procedure involving half dose—1 h stabilization—half dose. The A point in the graph is relative to the last injection. After 4 h from the injection of the α -syn, the monomers do not cause a toxic reaction while the crude mixture had led the cells to death. More than 15 independent repeats were completed for each case. The histograms depict the average variance and the error bars indicate the variability of the variance over the chosen time-step.

    Journal: Cell Death Discovery

    Article Title: Amyloid single-cell cytotoxicity assays by nanomotion detection

    doi: 10.1038/cddiscovery.2017.53

    Figure Lengend Snippet: Nanomotion single-cell cytotoxicity studies. Typical nanomotion response of M17 neuroblastoma cells exposed to ( a ) monomeric α -syn and ( b ) the crude mixture of oligomers and fibrils. In both cases, the amyloid species were injected in a two-step procedure involving half dose—1 h stabilization—half dose. The A point in the graph is relative to the last injection. After 4 h from the injection of the α -syn, the monomers do not cause a toxic reaction while the crude mixture had led the cells to death. More than 15 independent repeats were completed for each case. The histograms depict the average variance and the error bars indicate the variability of the variance over the chosen time-step.

    Article Snippet: Preparation and characterization of α -syn recombinant proteins: preparation of crude mixture The α -syn crude mixture was generated incubating 800 μ l of a 45 μ M filtered (100 kDa filter) (Millipore) monomeric α -syn solution (50 mM Tris, 150 mM NaCl, pH 7.5) under constant orbital agitation (400 rpm) at 37 °C for 4 weeks.

    Techniques: Injection

    Differential expression of PKG-I isoforms during fat cell differentiation. ( A ) Images of human omental preadipocytes undergoing differentiation into adipocytes taken with phase contrast microscopy (left panels) and coherent anti-Stokes Raman scattering (CARS) microscopy (right panels). ( B ) Increased phosphorylation of endothelial nitric oxide synthase (eNOS) at Serine residue 1177 detected with 1D Western blots. ( C ) Expression of both PKG-Iα and PKG-Iβ isoforms in preadipocytes (solid line) and expression of only PKG-Iβ isoform in adipocytes (dashed line). ( D ) Normalized ratios of PKG-Iα/PKG-Iβ as a function of cell differentiation at day 0 (preadipocytes) and day 8 (adipocytes). Error bars are standard deviation values of triplicate measurements. Asterisk indicates p-value

    Journal: PLoS ONE

    Article Title: Capillary Isoelectric Focusing Immunoassay for Fat Cell Differentiation Proteomics

    doi: 10.1371/journal.pone.0132105

    Figure Lengend Snippet: Differential expression of PKG-I isoforms during fat cell differentiation. ( A ) Images of human omental preadipocytes undergoing differentiation into adipocytes taken with phase contrast microscopy (left panels) and coherent anti-Stokes Raman scattering (CARS) microscopy (right panels). ( B ) Increased phosphorylation of endothelial nitric oxide synthase (eNOS) at Serine residue 1177 detected with 1D Western blots. ( C ) Expression of both PKG-Iα and PKG-Iβ isoforms in preadipocytes (solid line) and expression of only PKG-Iβ isoform in adipocytes (dashed line). ( D ) Normalized ratios of PKG-Iα/PKG-Iβ as a function of cell differentiation at day 0 (preadipocytes) and day 8 (adipocytes). Error bars are standard deviation values of triplicate measurements. Asterisk indicates p-value

    Article Snippet: Recombinant proteins Recombinant PKG-I isoforms, PKG-Iβ (Cat. No. 14–650) and PKG-Iα (Cat. No. 14-688M) were purchased from Millipore (Billerica, MA).

    Techniques: Expressing, Cell Differentiation, Microscopy, Western Blot, Standard Deviation

    Differential expression of PKG-I isoforms in cell lines and tissues. ( A ) Human pancreatic islets exhibited only the presence of PKG-Iβ. ( B ) Human umbilical vascular endothelial cells (HUVEC) exhibited both PKG-Iα and PKG-Iβ. ( C ) Mammary cancer cells MCF-7 exhibited only PKG-Iα.

    Journal: PLoS ONE

    Article Title: Capillary Isoelectric Focusing Immunoassay for Fat Cell Differentiation Proteomics

    doi: 10.1371/journal.pone.0132105

    Figure Lengend Snippet: Differential expression of PKG-I isoforms in cell lines and tissues. ( A ) Human pancreatic islets exhibited only the presence of PKG-Iβ. ( B ) Human umbilical vascular endothelial cells (HUVEC) exhibited both PKG-Iα and PKG-Iβ. ( C ) Mammary cancer cells MCF-7 exhibited only PKG-Iα.

    Article Snippet: Recombinant proteins Recombinant PKG-I isoforms, PKG-Iβ (Cat. No. 14–650) and PKG-Iα (Cat. No. 14-688M) were purchased from Millipore (Billerica, MA).

    Techniques: Expressing

    Detection of PKG-I isoforms using 1D and 2D Western blots and cIEF immunoassays. ( A ) 1D Western blot image (upper panel) and chemiluminescent intensity as a function of molecular mass plot (lower panel). ( B ) Truncated 2D Western blot images (upper panels) and chemiluminescent intensity as a function of isoelectric points plot (lower panel). ( C ) cIEF immunoassay images (upper panels) and chemiluminescent intensity as a function of isoelectric points plot (lower panel).

    Journal: PLoS ONE

    Article Title: Capillary Isoelectric Focusing Immunoassay for Fat Cell Differentiation Proteomics

    doi: 10.1371/journal.pone.0132105

    Figure Lengend Snippet: Detection of PKG-I isoforms using 1D and 2D Western blots and cIEF immunoassays. ( A ) 1D Western blot image (upper panel) and chemiluminescent intensity as a function of molecular mass plot (lower panel). ( B ) Truncated 2D Western blot images (upper panels) and chemiluminescent intensity as a function of isoelectric points plot (lower panel). ( C ) cIEF immunoassay images (upper panels) and chemiluminescent intensity as a function of isoelectric points plot (lower panel).

    Article Snippet: Recombinant proteins Recombinant PKG-I isoforms, PKG-Iβ (Cat. No. 14–650) and PKG-Iα (Cat. No. 14-688M) were purchased from Millipore (Billerica, MA).

    Techniques: Western Blot

    Far western Immunoblotting of BLM termini. a Left panel Conventional western analysis of the BLM fragments using anti-FLAG M2 antibody. Right panel Far western analysis of BLM fragments interaction with RAD51 using anti-RAD51 antibody. b Left panel Far

    Journal: The Protein Journal

    Article Title: Critical Interaction Domains between Bloom Syndrome Protein and RAD51

    doi: 10.1007/s10930-010-9295-8

    Figure Lengend Snippet: Far western Immunoblotting of BLM termini. a Left panel Conventional western analysis of the BLM fragments using anti-FLAG M2 antibody. Right panel Far western analysis of BLM fragments interaction with RAD51 using anti-RAD51 antibody. b Left panel Far

    Article Snippet: Conventional western blotting was then performed to detect the presence of RAD51 protein using anti-RAD51, 1:1,000 dilution (EMD Chemicals, Inc, Gibbstown, NJ) and anti-Mouse IgG conjugated horseradish peroxidase, 1:10,000 dilution.

    Techniques: Western Blot

    Schematic of BLM fragments generated. a Full length BLM protein with regions that interact with RAD51 marked. b All fragments generated include an N-terminal 6× Histidine epitope tag and a C-terminal FLAG epitope tag. Exact amino acids in each

    Journal: The Protein Journal

    Article Title: Critical Interaction Domains between Bloom Syndrome Protein and RAD51

    doi: 10.1007/s10930-010-9295-8

    Figure Lengend Snippet: Schematic of BLM fragments generated. a Full length BLM protein with regions that interact with RAD51 marked. b All fragments generated include an N-terminal 6× Histidine epitope tag and a C-terminal FLAG epitope tag. Exact amino acids in each

    Article Snippet: Conventional western blotting was then performed to detect the presence of RAD51 protein using anti-RAD51, 1:1,000 dilution (EMD Chemicals, Inc, Gibbstown, NJ) and anti-Mouse IgG conjugated horseradish peroxidase, 1:10,000 dilution.

    Techniques: Generated, FLAG-tag

    Representation of BLM interaction domains with RAD51 and ssDNA

    Journal: The Protein Journal

    Article Title: Critical Interaction Domains between Bloom Syndrome Protein and RAD51

    doi: 10.1007/s10930-010-9295-8

    Figure Lengend Snippet: Representation of BLM interaction domains with RAD51 and ssDNA

    Article Snippet: Conventional western blotting was then performed to detect the presence of RAD51 protein using anti-RAD51, 1:1,000 dilution (EMD Chemicals, Inc, Gibbstown, NJ) and anti-Mouse IgG conjugated horseradish peroxidase, 1:10,000 dilution.

    Techniques: