Recombinant Influenza Ha Proteins, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 4 article reviews
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1) Product Images from "Simultaneous Targeting of Multiple Hemagglutinins to APCs for Induction of Broad Immunity against Influenza"
Article Title: Simultaneous Targeting of Multiple Hemagglutinins to APCs for Induction of Broad Immunity against Influenza
Journal: The Journal of Immunology Author Choice
Figure Legend Snippet: Increased strain-specific IgG titers after a single MHCII-targeted vaccination. BALB/c mice ( n = 6 per group) were immunized once with 25 μg of plasmids encoding one of the indicated vaccines. ( A ) Sera were harvested at weeks 2, 3, and 4 after vaccination, and IgG responses in sera from each of the vaccine groups were measured by ELISAs against recombinant HA from H5, H6, H8, H9, H11, H13, and H1 (PR8) influenza viruses (mean ± SEM). ** p
Techniques Used: Mouse Assay, Recombinant
Figure Legend Snippet: Ab responses against all included HAs after DNA immunization with vaccine mixtures. BALB/c mice ( n = 6 per group) were immunized three times (weeks 0, 4, and 12) with 25 μg of DNA of the indicated vaccine (arrowheads). Serum samples were harvested 2–3 wk after each vaccine delivery, and HA-specific IgG responses were measured in sandwich ELISAs against recombinant HA from H5, H6, H8, H9, H11, and H13 influenza viruses (mean ± SEM). * p
Techniques Used: Mouse Assay, Recombinant
2) Product Images from "Pan-Influenza A Protection by Prime–Boost Vaccination with Cold-Adapted Live-Attenuated Influenza Vaccine in a Mouse Model"
Article Title: Pan-Influenza A Protection by Prime–Boost Vaccination with Cold-Adapted Live-Attenuated Influenza Vaccine in a Mouse Model
Journal: Frontiers in Immunology
Figure Legend Snippet: Cross-reactive hemagglutinin (HA)-specific antibody responses elicited by vaccination. (A) Breadth of the HA-specific sera IgG antibodies. To examine the breadth of HA-specific sera IgG antibodies induced by vaccination, recombinant HA proteins expressed in insect cells were used as coating antigens in ELISA. The HA proteins tested include five different group 1 HAs from H1N1 (A/California/6/2009), H1N1 (A/Puerto Rico/8/1934), H2N2 (A/Canada/720/2006), H5N1 (A/Indonesia/5/2005), and H9N2 (A/Hong Kong/35820/2009) and two group 2 HAs from H3N2 (A/Sydney/5/1997) and H7N9 (A/Anhui/1/2013) influenza viruses. (B) Antibody titers specific to HA full-length or stalk of pH1N1 or PR8 (H1N1) virus. Using the Escherichia coli -expressed LysRS-HA fusion proteins as coating antigens, sera IgG antibodies specific to the HA full-length or stalk protein were measured by ELISA. Antibody titers were expressed as the reciprocal serum dilution that yielded OD 450 greater than the mean + 2 SD (SD) of PBS control group. Data are the mean of each cohort ( N = 5), and error bars indicate SD. *** P
Techniques Used: Recombinant, Enzyme-linked Immunosorbent Assay
Figure Legend Snippet: Experimental design of vaccination and challenge in mice. (A) Vaccination schedule and design of prime–boost vaccination against heterologous challenge. Four different prime–boost vaccination groups were designed using three different cold-adapted, live attenuated influenza vaccines (CAIVs), ca-pH1N1, ca-NCH1N1, and ca-IDH5N1. Prime and boost CAIVs (10 5 PFUs of each vaccine) were administered into mice via intranasal route with two weeks interval. A month later, each group was divided into four subgroups (20 subgroups in total) and challenged with 10 mouse lethal dose 50 (MLD 50 ) of each of four heterologous influenza viruses. (B) Phylogenetic tree of influenza A hemagglutinin (HA) proteins. HA to which binding affinity of vaccination-induced antibodies or protection efficacy in vivo tested in this study was highlighted in colored circles.
Techniques Used: Mouse Assay, Binding Assay, In Vivo