recombinant influenza a virus h5n1  (Sino Biological)


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    Name:
    Influenza A H5N1 A Anhui 1 2005 Hemagglutinin HA Protein His Fc Tag
    Description:
    A DNA sequence encoding the extracellular domain of Influenza A virus A Anhui 1 2005 H5N1 ABD28180 1 hemagglutinin Met 1 Gln 530 with cleavage site mutated HA1 HA2 uncleaved was fused with the C terminal polyhistidine tagged Fc region of mouse IgG1 at the C terminus
    Catalog Number:
    11048-V06H1
    Price:
    None
    Category:
    recombinant protein
    Host:
    HEK293 Cells
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    Structured Review

    Sino Biological recombinant influenza a virus h5n1
    A DNA sequence encoding the extracellular domain of Influenza A virus A Anhui 1 2005 H5N1 ABD28180 1 hemagglutinin Met 1 Gln 530 with cleavage site mutated HA1 HA2 uncleaved was fused with the C terminal polyhistidine tagged Fc region of mouse IgG1 at the C terminus
    https://www.bioz.com/result/recombinant influenza a virus h5n1/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant influenza a virus h5n1 - by Bioz Stars, 2021-07
    94/100 stars

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    Related Articles

    Recombinant:

    Article Title: A Homogenous Fluorescence Quenching Based Assay for Specific and Sensitive Detection of Influenza Virus A Hemagglutinin Antigen
    Article Snippet: ReagentsCarboxyl quantum dots (QD655) were obtained from Life Technologies Inc. (Carlsbad, CA, USA). .. Recombinant influenza A virus H1N1 (A/New Caledonia/20/1999) HA, recombinant influenza A virus H5N1 (A/Viet Nam/1194/2004) HA, mouse monoclonal antibody to influenza A virus H1N1 HA (mAb-H1-HA), and mouse monoclonal antibody to influenza A virus H5N1 HA (mAb-H5-HA) were purchased from Sino Biological Inc. (Beijing, China). .. Two glycan, 6'-sialyllactose sodium salt (hereafter denoted as 6G) and 3'-sialyllactose sodium salt (hereafter denoted as 3G), were ordered from Carbosynth Limited Inc. (Berkshire, UK).

    Article Title: Neuraminidase inhibition of Dietary chlorogenic acids and derivatives - potential antivirals from dietary sources.
    Article Snippet: Plants rich in chlorogenic acids (CGAs), caffeic acids and their derivatives have been found to exert antiviral effects against influenza virus neuroaminidase. .. Plants rich in chlorogenic acids (CGAs), caffeic acids and their derivatives have been found to exert antiviral effects against influenza virus neuroaminidase. ..

    other:

    Article Title: Upconversion Fluorescence Resonance Energy Transfer Aptasensors for H5N1 Influenza Virus Detection
    Article Snippet: Influenza A H5N1 HA protein was purchased from Sino Biological Inc. (Beijing, China).

    Plasmid Preparation:

    Article Title: A novel neutralizing antibody against diverse clades of H5N1 influenza virus and its mutants capable of airborne transmission.
    Article Snippet: .. Highly pathogenic avian influenza A virus H5N1 continues to spread among poultry and has frequently broken the species barrier to humans. ..

    Expressing:

    Article Title: A novel neutralizing antibody against diverse clades of H5N1 influenza virus and its mutants capable of airborne transmission.
    Article Snippet: .. Highly pathogenic avian influenza A virus H5N1 continues to spread among poultry and has frequently broken the species barrier to humans. ..

    SPR Assay:

    Article Title: Synthesis of Four Pentacyclic Triterpene–Sialylglycopeptide Conjugates and Their Affinity Assays with Hemagglutinin
    Article Snippet: C135 H217 N11 O74 , ESI-MS (negative mode, m/z ): 1588.2 [M − 2H]2− , 1058.5 [M − 3H]3− ; HR-ESI-MS (positive mode, m/z ) 1589.1852 [M + 2H]2+ (calcd 1589.1850); 1 H-NMR (600 MHz, CD3 OD) δ 5.61 (s, 1H, CH-12), 5.13 (s, 1H, H-1 of Man-4), 4.34–4.33 (m, 2H, H-1 of Gal-6,Gal-6’), 3.00 (m, 2H, β-CH2 of Asn), 2.60 (m, 2H, H-3eq of NeuAc, NeuAc’), 1.42 (s, 3H, CH3 -27), 1.16 (s, 3H, CH3 -29), 1.14 (s, 6H, CH3 -26, 25), 0.99 (s, 3H, CH3 -23), 0.83 (s, 3H, CH3 -24), 0.80 (s, 3H, CH3-28). .. SPR Assay Biacore T200 instruments (GE Healthcare) were used to evaluate the binding affinity of compounds with H1N1 (A/WSN/1933) and H5N1 (A/Hong Kong/483/97), purchased from Sino Biological Inc. (Beijing, China) through SPR, as previously described [ ]. .. In brief, HA protein was immobilized on the surface of a CM5 chip by using the amine coupling approach at a flow rate of 10 μL/min in 10 mM sodium acetate buffer (pH 5.0).

    Binding Assay:

    Article Title: Synthesis of Four Pentacyclic Triterpene–Sialylglycopeptide Conjugates and Their Affinity Assays with Hemagglutinin
    Article Snippet: C135 H217 N11 O74 , ESI-MS (negative mode, m/z ): 1588.2 [M − 2H]2− , 1058.5 [M − 3H]3− ; HR-ESI-MS (positive mode, m/z ) 1589.1852 [M + 2H]2+ (calcd 1589.1850); 1 H-NMR (600 MHz, CD3 OD) δ 5.61 (s, 1H, CH-12), 5.13 (s, 1H, H-1 of Man-4), 4.34–4.33 (m, 2H, H-1 of Gal-6,Gal-6’), 3.00 (m, 2H, β-CH2 of Asn), 2.60 (m, 2H, H-3eq of NeuAc, NeuAc’), 1.42 (s, 3H, CH3 -27), 1.16 (s, 3H, CH3 -29), 1.14 (s, 6H, CH3 -26, 25), 0.99 (s, 3H, CH3 -23), 0.83 (s, 3H, CH3 -24), 0.80 (s, 3H, CH3-28). .. SPR Assay Biacore T200 instruments (GE Healthcare) were used to evaluate the binding affinity of compounds with H1N1 (A/WSN/1933) and H5N1 (A/Hong Kong/483/97), purchased from Sino Biological Inc. (Beijing, China) through SPR, as previously described [ ]. .. In brief, HA protein was immobilized on the surface of a CM5 chip by using the amine coupling approach at a flow rate of 10 μL/min in 10 mM sodium acetate buffer (pH 5.0).

    Infection:

    Article Title: The N-linked glycosylation site at position 158 on the head of hemagglutinin and the virulence of H5N1 avian influenza virus in mice.
    Article Snippet: N-linked glycosylation of the influenza virus hemagglutinin (HA) protein plays crucial roles in HA structure and function, evasion of neutralizing antibodies, and susceptibility to innate soluble antiviral factors. .. N-linked glycosylation of the influenza virus hemagglutinin (HA) protein plays crucial roles in HA structure and function, evasion of neutralizing antibodies, and susceptibility to innate soluble antiviral factors. ..

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  • 93
    Sino Biological anti h7n9 hemagglutinin ha antibody rabbit pab
    Biological and chemical modifications of <t>H7N9</t> VLPs. (A) SDS-PAGE and western blot analysis of biologically modified H7N9 VLPs. Lane 1–3 represent 320 ng, 180 ng and 90 ng of αGal (-) VLP based on HA content, respectively. Lane 4–6 are 130 ng, 90, and 40 ng of αGal (+) VLP, respectively. (B) SDS-PAGE and western blot analysis of chemically modified H7N9 VLPs. S: protein standards; 1: αGal (-) H7N9 VLP; 2: intermediate of αGal (-) H7N9 VLP reacted with GO; 3: H7N9 conjugated with αGal linker a11; 4: H7N9 conjugated with αGal linker aN11; 5: H7N9 VLPs conjugated with control linker sp11. (C) Electron microscopy of H7N9 VLPs. (D) Electron microscopy of H7N9 VLPs stained with gold particles. Negative: VLPs were only stained with immunogold donkey anti-rabbit secondary antibody; Positive: VLPs were stained with anti-HA7 rabbit polyclonal antibody, followed by staining with immunogold donkey anti-rabbit secondary antibody. Sp11 is the aminooxy spacer control linker without αGal antigen; a11 is one type of αGal (Galα1,3-Galβ1,4-Glc) aminooxy linker; aN11 is the other type of αGal (Galα1,3-Galβ1,4-GlcNAc) aminooxy linker.
    Anti H7n9 Hemagglutinin Ha Antibody Rabbit Pab, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti h7n9 hemagglutinin ha antibody rabbit pab/product/Sino Biological
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti h7n9 hemagglutinin ha antibody rabbit pab - by Bioz Stars, 2021-07
    93/100 stars
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    92
    Sino Biological influenza a h5n1 hemagglutinin ha antibody rabbit mab
    Expression of viral proteins in infected MDCK cells. MDCK cells were infected with viruses at an MOI of 5. The infected cells were collected at 12 h postinfection, lysed in SDS loading buffer, and further analyzed by Western blotting (A and B). (A) Protein bands of HA, NP, and M1 proteins detected by Western blotting. The intensity of each band was measured by using ImageJ software, and relative intensity ratios of HA, NP, and M1 compared with that of GAPDH were calculated (B). The infected cells were digested with trypsin to obtain a single cell suspension at 12 h postinfection. The samples were stained with rabbit monoclonal antibody to <t>influenza</t> A virus <t>H5N1</t> HA protein and Alexa Fluor 488-conjugated goat anti-rabbit IgG (H+L) secondary antibody and then detected on a FACSAria II (BD Biosciences). (C) Mean fluorescence intensity of HA protein was analyzed with FlowJo X 10.0.7r2 (Tree Star, San Carlos, CA). Data are presented as means ± SD ( n = 3). **, P
    Influenza A H5n1 Hemagglutinin Ha Antibody Rabbit Mab, supplied by Sino Biological, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/influenza a h5n1 hemagglutinin ha antibody rabbit mab/product/Sino Biological
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    influenza a h5n1 hemagglutinin ha antibody rabbit mab - by Bioz Stars, 2021-07
    92/100 stars
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    94
    Sino Biological recombinant influenza a virus h5n1 hemagglutinin
    HA-based vaccines stimulate antigen-specific antibody responses in BALB/c mice. a Schematic representation of the standard immunization schedule (priming + two boosters). b Antigen-specific total IgG titer in sera collected on day 30 from BALB/c mice subjected to i.m. immunization with different antigens conjugated to 200 kDa HA or injected alone (standard immunization schedule; BSA, Vк 3-20 , OVA, n = 12; SOD, hGH, TT, RABV G, n = 6; <t>H5N1,</t> n = 4). c Anti-OVA total IgG and IgG subclass titers detected on day 30 in sera of BALB/c mice subjected to i.m. immunization with 10 μg of OVA alone, conjugated to HA, or emulsified with alum following the standard schedule ( n = 10 mice/group; HA vs. alum: P
    Recombinant Influenza A Virus H5n1 Hemagglutinin, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant influenza a virus h5n1 hemagglutinin/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant influenza a virus h5n1 hemagglutinin - by Bioz Stars, 2021-07
    94/100 stars
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    88
    Sino Biological influenza a h5n1 hemagglutinin ha antibody rabbit pab
    MAb-mediated protection against a lethal challenge of <t>H5N1</t> virus in mice
    Influenza A H5n1 Hemagglutinin Ha Antibody Rabbit Pab, supplied by Sino Biological, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/influenza a h5n1 hemagglutinin ha antibody rabbit pab/product/Sino Biological
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    influenza a h5n1 hemagglutinin ha antibody rabbit pab - by Bioz Stars, 2021-07
    88/100 stars
      Buy from Supplier

    Image Search Results


    Biological and chemical modifications of H7N9 VLPs. (A) SDS-PAGE and western blot analysis of biologically modified H7N9 VLPs. Lane 1–3 represent 320 ng, 180 ng and 90 ng of αGal (-) VLP based on HA content, respectively. Lane 4–6 are 130 ng, 90, and 40 ng of αGal (+) VLP, respectively. (B) SDS-PAGE and western blot analysis of chemically modified H7N9 VLPs. S: protein standards; 1: αGal (-) H7N9 VLP; 2: intermediate of αGal (-) H7N9 VLP reacted with GO; 3: H7N9 conjugated with αGal linker a11; 4: H7N9 conjugated with αGal linker aN11; 5: H7N9 VLPs conjugated with control linker sp11. (C) Electron microscopy of H7N9 VLPs. (D) Electron microscopy of H7N9 VLPs stained with gold particles. Negative: VLPs were only stained with immunogold donkey anti-rabbit secondary antibody; Positive: VLPs were stained with anti-HA7 rabbit polyclonal antibody, followed by staining with immunogold donkey anti-rabbit secondary antibody. Sp11 is the aminooxy spacer control linker without αGal antigen; a11 is one type of αGal (Galα1,3-Galβ1,4-Glc) aminooxy linker; aN11 is the other type of αGal (Galα1,3-Galβ1,4-GlcNAc) aminooxy linker.

    Journal: PLoS ONE

    Article Title: Addition of αGal HyperAcute™ technology to recombinant avian influenza vaccines induces strong low-dose antibody responses

    doi: 10.1371/journal.pone.0182683

    Figure Lengend Snippet: Biological and chemical modifications of H7N9 VLPs. (A) SDS-PAGE and western blot analysis of biologically modified H7N9 VLPs. Lane 1–3 represent 320 ng, 180 ng and 90 ng of αGal (-) VLP based on HA content, respectively. Lane 4–6 are 130 ng, 90, and 40 ng of αGal (+) VLP, respectively. (B) SDS-PAGE and western blot analysis of chemically modified H7N9 VLPs. S: protein standards; 1: αGal (-) H7N9 VLP; 2: intermediate of αGal (-) H7N9 VLP reacted with GO; 3: H7N9 conjugated with αGal linker a11; 4: H7N9 conjugated with αGal linker aN11; 5: H7N9 VLPs conjugated with control linker sp11. (C) Electron microscopy of H7N9 VLPs. (D) Electron microscopy of H7N9 VLPs stained with gold particles. Negative: VLPs were only stained with immunogold donkey anti-rabbit secondary antibody; Positive: VLPs were stained with anti-HA7 rabbit polyclonal antibody, followed by staining with immunogold donkey anti-rabbit secondary antibody. Sp11 is the aminooxy spacer control linker without αGal antigen; a11 is one type of αGal (Galα1,3-Galβ1,4-Glc) aminooxy linker; aN11 is the other type of αGal (Galα1,3-Galβ1,4-GlcNAc) aminooxy linker.

    Article Snippet: Following a 30-minute incubation in a glass petri dish incubation chamber, the grid was stained in a 40 μL drop of rabbit polyclonal anti-HA7 antibody (Sino Biological Inc., 40103-RP02, 1: 500 dilution in blocking buffer) or blocking buffer alone to serve as a negative control.

    Techniques: SDS Page, Western Blot, Modification, Electron Microscopy, Staining

    Expression of viral proteins in infected MDCK cells. MDCK cells were infected with viruses at an MOI of 5. The infected cells were collected at 12 h postinfection, lysed in SDS loading buffer, and further analyzed by Western blotting (A and B). (A) Protein bands of HA, NP, and M1 proteins detected by Western blotting. The intensity of each band was measured by using ImageJ software, and relative intensity ratios of HA, NP, and M1 compared with that of GAPDH were calculated (B). The infected cells were digested with trypsin to obtain a single cell suspension at 12 h postinfection. The samples were stained with rabbit monoclonal antibody to influenza A virus H5N1 HA protein and Alexa Fluor 488-conjugated goat anti-rabbit IgG (H+L) secondary antibody and then detected on a FACSAria II (BD Biosciences). (C) Mean fluorescence intensity of HA protein was analyzed with FlowJo X 10.0.7r2 (Tree Star, San Carlos, CA). Data are presented as means ± SD ( n = 3). **, P

    Journal: Journal of Virology

    Article Title: Glycosylation of the Hemagglutinin Protein of H5N1 Influenza Virus Increases Its Virulence in Mice by Exacerbating the Host Immune Response

    doi: 10.1128/JVI.02215-16

    Figure Lengend Snippet: Expression of viral proteins in infected MDCK cells. MDCK cells were infected with viruses at an MOI of 5. The infected cells were collected at 12 h postinfection, lysed in SDS loading buffer, and further analyzed by Western blotting (A and B). (A) Protein bands of HA, NP, and M1 proteins detected by Western blotting. The intensity of each band was measured by using ImageJ software, and relative intensity ratios of HA, NP, and M1 compared with that of GAPDH were calculated (B). The infected cells were digested with trypsin to obtain a single cell suspension at 12 h postinfection. The samples were stained with rabbit monoclonal antibody to influenza A virus H5N1 HA protein and Alexa Fluor 488-conjugated goat anti-rabbit IgG (H+L) secondary antibody and then detected on a FACSAria II (BD Biosciences). (C) Mean fluorescence intensity of HA protein was analyzed with FlowJo X 10.0.7r2 (Tree Star, San Carlos, CA). Data are presented as means ± SD ( n = 3). **, P

    Article Snippet: The samples were stained with a rabbit monoclonal antibody to influenza A virus H5N1 HA protein (11048-RM07; Sino Biological Inc.) and with Alexa Fluor 488-conjugated goat anti-rabbit IgG (H+L) secondary antibody (A-11034; Thermo Fisher Scientific).

    Techniques: Expressing, Infection, Western Blot, Software, Staining, Fluorescence

    HA-based vaccines stimulate antigen-specific antibody responses in BALB/c mice. a Schematic representation of the standard immunization schedule (priming + two boosters). b Antigen-specific total IgG titer in sera collected on day 30 from BALB/c mice subjected to i.m. immunization with different antigens conjugated to 200 kDa HA or injected alone (standard immunization schedule; BSA, Vк 3-20 , OVA, n = 12; SOD, hGH, TT, RABV G, n = 6; H5N1, n = 4). c Anti-OVA total IgG and IgG subclass titers detected on day 30 in sera of BALB/c mice subjected to i.m. immunization with 10 μg of OVA alone, conjugated to HA, or emulsified with alum following the standard schedule ( n = 10 mice/group; HA vs. alum: P

    Journal: Cellular and Molecular Immunology

    Article Title: Hyaluronan is a natural and effective immunological adjuvant for protein-based vaccines

    doi: 10.1038/s41423-021-00667-y

    Figure Lengend Snippet: HA-based vaccines stimulate antigen-specific antibody responses in BALB/c mice. a Schematic representation of the standard immunization schedule (priming + two boosters). b Antigen-specific total IgG titer in sera collected on day 30 from BALB/c mice subjected to i.m. immunization with different antigens conjugated to 200 kDa HA or injected alone (standard immunization schedule; BSA, Vк 3-20 , OVA, n = 12; SOD, hGH, TT, RABV G, n = 6; H5N1, n = 4). c Anti-OVA total IgG and IgG subclass titers detected on day 30 in sera of BALB/c mice subjected to i.m. immunization with 10 μg of OVA alone, conjugated to HA, or emulsified with alum following the standard schedule ( n = 10 mice/group; HA vs. alum: P

    Article Snippet: Different antigens, i.e. ovalbumin (OVA, Hyglos GmbH); human superoxide dismutase (SOD), bovine serum albumin (BSA), human growth hormone (hGH, Sigma-Aldrich); tetanus toxoid (TT, Alomone Labs); recombinant influenza A virus H5N1 hemagglutinin (H5N1, Sino Biological Inc.); a recombinant immunoglobulin (Ig) light κ chain variable region (Vκ3–20 , a kind gift from Prof. R. Dolcetti, Centro di Riferimento Oncologico di Aviano (CRO-IRCCS), Aviano, Italy); and rabies virus G glycoprotein (RABV G; obtained from Challenge Virus Strain—11 (ATCC® VR-959™), propagated in BHK-21 cells (ATCC® CCL-10™), and purified as previously described ), were conjugated to HA-acetal using a general procedure for each antigen.

    Techniques: Mouse Assay, Injection

    MAb-mediated protection against a lethal challenge of H5N1 virus in mice

    Journal: Antiviral research

    Article Title: Selection of Therapeutic H5N1 Monoclonal Antibodies Following IgVH Repertoire Analysis in Mice

    doi: 10.1016/j.antiviral.2016.04.001

    Figure Lengend Snippet: MAb-mediated protection against a lethal challenge of H5N1 virus in mice

    Article Snippet: Hemagglutination inhibition (HI) activity specific to A/Vietnam/1194/2004 (H5N1), A/Cambodia/RO405050/2007 (H5N1), A/turkey/Turkey/1/2005 (H5N1), and A/H5N1/Anhui/1/05 (H5N1) virus (NIBSC, Hertfordshire England) were determined ( ).

    Techniques: Mouse Assay