Journal: Cancer Immunology Research

Article Title: T Cells Expressing a Modified FcγRI Exert Antibody-Dependent Cytotoxicity and Overcome the Limitations of CAR T-cell Therapy against Solid Tumors

doi: 10.1158/2326-6066.CIR-22-0423

Figure Lengend Snippet: Expression, cytotoxic activity, and cytokine secretion of T cells expressing altered version of FcγRI. A, Illustration of the modified FcγRI structure with fused signaling domains: A2G (left), FcγRI composed of α-chain and homodimer of FcRγ chain; AG2G (middle), FcγRI α-chain with fused domain of FcRγ chain and homodimer of FcRγ; AGO2GO (right), FcγRI α-chain with fused FcRγ and OX40 domains and homodimer of FcRγ with fused OX40 domain. B, Flow cytometry analysis of A2G, AG2G, and AGO2GO expression following retroviral transduction of healthy donor T cells. C, Mean numbers of HT29 target cells expressing HER2 calculated by incuCyte software through 48-hour incubation with the three FcγRI-based receptor expressing T cells, with 1:1 E:T ratios with or without 6 μg/mL trastuzumab (data shows one of two experiments repeats, n = 4). Each sample is normalized to its cell number at time zero. Statistical significance was calculated using two-way ANOVA with Sidak correction for multiple comparisons. D, Representative incuCyte images showing HER2-expressing HT29 cells, imaged and counted in live imaging system after 48 hours of incubation with different construct-expressing T cells with 1:1 E:T ratio. Scale bar is 100 μm. E, Human Luminex Discovery Assay measurement of cytokine levels in supernatants from 48 hours of culture of T cells 4:1 E:T ratio with HT29 cells expressing HER2 ( n = 4). Graphs show mean ±SD. Statistical significance was calculated using two-way ANOVA with Tukey's correction for multiple comparisons. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. Error bars represent standard error.

Article Snippet: For activation of effector cells on plastic-bound ligands, monoclonal antibodies or recombinant Human HER2/ErbB2 Protein (ECD) Biotinylated (Sino Biological, #10004-HCCH-B) were incubated on Maxisorp ELISA 96-well plates (Nunc Thermos Fisher Scientific) in PBS for 24 hours, washed three times and 10 5 T cells were incubated for 24 hours in 150 μL RPMI medium supplemented with 10% FBS.

Techniques: Expressing, Activity Assay, Modification, Flow Cytometry, Transduction, Software, Incubation, Imaging, Construct, Luminex

Journal: Cancer Immunology Research

Article Title: T Cells Expressing a Modified FcγRI Exert Antibody-Dependent Cytotoxicity and Overcome the Limitations of CAR T-cell Therapy against Solid Tumors

doi: 10.1158/2326-6066.CIR-22-0423

Figure Lengend Snippet: Characterization of AG2G-expressing T cells, activation, and cytotoxicity. A, ELISA measurement of cytokine levels of AG2G-expressing T cells after 48-hour incubation with HT29 cells expressing HER2. B, IFNγ and TNFα levels measured by ELISA in supernatants from 48-hour incubation of AG2G-expressing T cells and HT29 cells expressing HER2 in different E:T ratios. C, Mean numbers of HT29 cells expressing HER2, calculated by incuCyte software, following incubation with AG2G-expressing T cells in combination with 0, 2.5, 5, 10 mg/mL IVIG, with or without 30 μg/mL trastuzumab ( n = 4). D, ELISA measurement of IFNγ levels of AG2G-expressing cells cocultured with HT29 cells expressing HER2 described in B . E, Mean numbers of HT29 cells expressing HER2, calculated by incuCyte software, following incubation with AG2G-expressing T cells in combination with different concentrations of trastuzumab over 96 hours ( n = 2). F, Mean numbers of HT29 cells expressing HER2, calculated by incuCyte software, following incubation with different E:T ratios of AG2G-expressing T cells that were isolated for their CD4 (left) or CD8 (middle) population or with no isolation (right). G, ELISA measurement of cytokine levels from supernatants of 48-hour culture of 4:1 E:T ratios of isolated CD4, isolated CD8, or AG2G-expressing T cells with HT29 cells expressing HER2 and 30 μg/mL trastuzumab. Graphs show mean ±SD. Statistical significance was calculated using Student t test for a, two-way ANOVA with Sidak correction for multiple comparisons for B and C , two-way ANOVA with Tukey's correction for multiple comparisons for E and F . *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. Error bars represent standard error.

Article Snippet: For activation of effector cells on plastic-bound ligands, monoclonal antibodies or recombinant Human HER2/ErbB2 Protein (ECD) Biotinylated (Sino Biological, #10004-HCCH-B) were incubated on Maxisorp ELISA 96-well plates (Nunc Thermos Fisher Scientific) in PBS for 24 hours, washed three times and 10 5 T cells were incubated for 24 hours in 150 μL RPMI medium supplemented with 10% FBS.

Techniques: Expressing, Activation Assay, Enzyme-linked Immunosorbent Assay, Incubation, Software, Isolation

Journal: Cancer Immunology Research

Article Title: T Cells Expressing a Modified FcγRI Exert Antibody-Dependent Cytotoxicity and Overcome the Limitations of CAR T-cell Therapy against Solid Tumors

doi: 10.1158/2326-6066.CIR-22-0423

Figure Lengend Snippet: AG2G-expressing T cells differentiate between cells expressing high and low antigen levels, are more specific, and less exhausted, compared with classic CAR T cells. A, Mean numbers of HT29 cells expressing HER2, calculated by incuCyte software, following incubation with transduced T cells in combination with antibodies (60 μg/mL each, n = 4). B, ELISA measurement of IFNγ levels in 48-hour supernatants of AG2G-transduced T cells incubated with HER2-expressing HT29 target cells, and with tumor-binding trastuzumab, or irrelevant antibodies cetuximab and rituximab (60 μg/mL). C, Mean counts of HT29 cells expressing HER2, calculated by incuCyte software, following incubation with AG2G-transduced T cells and 30 μg/mL trastuzumab, compared with trastuzumab-derived CAR (4:1 E:T ratio, n = 4). Each sample is normalized to its cell number at time zero. D, Normalized confluence of kidney epithelial cells, calculated by incuCyte software, following incubation with AG2G-transduced T cells and 30 μg/mL trastuzumab, compared with trastuzumab-derived CAR (4:1 E:T ratio, n = 4). Each sample is normalized to its cell confluence at time zero. E, ELISA measurement of IFNγ levels in 48-hour supernatant obtained from transduced T cells and kidney epithel described in D ( n = 4). F, ELISA measurement of IFNγ, granzyme B and TNFα levels in 24-hour supernatants of 10 5 AG2G-expressing T cells or CAR T cells incubated on immobilized trastuzumab or rHER2, in rising concentrations (mmol/mL) respectively. G, Confocal microscope imaging of internalization kinetics of PE-labeled IgG by AG2G-expressing cells (top), or PE-labeled HER2 by CAR T cells (bottom). H, PD-1, LAG-3, and TIM3 flow cytometry analysis of AG2G-expressing cells or CAR T cells after 48-hour incubation with or without 12 mmol/mL immobilized trastuzumab or HER2, respectively. Graphs show mean ± SD. Statistical significance was calculated using two-way ANOVA with Tukey's correction for multiple comparisons for A , C , D , and H . Two-way ANOVA with Sidak correction for multiple comparisons for E and F . One-way ANOVA with Dunnett correction for multiple comparisons for B . *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. Error bars represent standard error.

Article Snippet: For activation of effector cells on plastic-bound ligands, monoclonal antibodies or recombinant Human HER2/ErbB2 Protein (ECD) Biotinylated (Sino Biological, #10004-HCCH-B) were incubated on Maxisorp ELISA 96-well plates (Nunc Thermos Fisher Scientific) in PBS for 24 hours, washed three times and 10 5 T cells were incubated for 24 hours in 150 μL RPMI medium supplemented with 10% FBS.

Techniques: Expressing, Software, Incubation, Enzyme-linked Immunosorbent Assay, Binding Assay, Derivative Assay, Microscopy, Imaging, Labeling, Flow Cytometry

Journal: Cancer Immunology Research

Article Title: T Cells Expressing a Modified FcγRI Exert Antibody-Dependent Cytotoxicity and Overcome the Limitations of CAR T-cell Therapy against Solid Tumors

doi: 10.1158/2326-6066.CIR-22-0423

Figure Lengend Snippet: Human T cells expressing AG2G exert specific tumor cytotoxicity while sparing normal cells. A, Flow cytometry expression analysis of HER2 on different tumor cell lines and primary human normal cells. B, ELISA measurement of IFNγ levels in 48-hour supernatants of AG2G-expressing T cells with different tumor cells or primary normal cells in 4:1 E:T ratio and 60 μg/mL trastuzumab (results shown are from 3 different donors combined, n = 12). C, Quantification of HER2 receptor numbers using DAKO QIFIKIT (Agilent) beads by flow cytometer. Individual populations of the calibration are gated, a linear regression is calculated from the MFI and the ABC values of the five calibration bead populations using MS Excel. Using the linear regression, the ABCs of the beads coated with αHER2 is calculated from their MFI values. SDs are also retrieved from gated populations and transformed using linear regression. D, Mean counts of HT29 cells expressing HER2, calculated by incuCyte software, following incubation with AG2G-transduced T cells or ACTR707 and 30 μg/mL trastuzumab (4:1 E:T ratio, n = 4). Each sample is normalized to its cell number at time zero. E, Normalized confluence of kidney epithelial (epithel) cells, calculated by incuCyte software, following incubation with AG2G-transduced T cells or ACTR707 and 30 μg/mL trastuzumab. Each sample is normalized to its cell confluence at time zero. Graphs are identical and described in . and but with the comparison to ACTR707 instead of CAR T. F, ELISA measurement of IFNγ levels in 48-hour supernatant obtained from transduced T cells and kidney epithel described in D and E ( n = 4). G, ELISA measurement of IFNγ levels in 24 hours supernatants of AG2G-expressing T cells or ACTR707 incubated with medium containing different concentration of IVIG ( n = 3). Graphs show mean ±SD. Statistical significance was calculated using two-way ANOVA with Sidak correction for multiple comparisons. ****, P < 0.0001. Error bars represent standard error. Endothel, endothelial cells.

Article Snippet: For activation of effector cells on plastic-bound ligands, monoclonal antibodies or recombinant Human HER2/ErbB2 Protein (ECD) Biotinylated (Sino Biological, #10004-HCCH-B) were incubated on Maxisorp ELISA 96-well plates (Nunc Thermos Fisher Scientific) in PBS for 24 hours, washed three times and 10 5 T cells were incubated for 24 hours in 150 μL RPMI medium supplemented with 10% FBS.

Techniques: Expressing, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Transformation Assay, Software, Incubation, Concentration Assay

Journal: Cancer Immunology Research

Article Title: T Cells Expressing a Modified FcγRI Exert Antibody-Dependent Cytotoxicity and Overcome the Limitations of CAR T-cell Therapy against Solid Tumors

doi: 10.1158/2326-6066.CIR-22-0423

Figure Lengend Snippet: Systemic administration of AG2G-expressing T cells in combination with trastuzumab eradicates HER2-expressing tumor cells in vivo . A, NCI-N87 tumor volume measured by caliper over 42 days following treatment initiation. NSG mice were injected with 2.5×10 6 NCI-N87 tumor cells, after tumor reached 70 mm 3 mice were randomized and treated once a week for 3 times (treatments are marked with arrows) with either intraperitoneal injection of saline or 250-μg trastuzumab and intravenous injection of saline or 5×10 6 AG2G-expressing T cells ( n = 6). B, Endpoint analysis of tumor volume measured by caliper, 42 days following treatment initiation. C, Endpoint analysis of tumor weight measured using scales, 42 days following treatment initiation. D, Histology images taken by x4 objective light microscopy, showing whole sections of tumors stained with H&E ( n = 6; N.D., not detected - 2 mice from AG2G+trastuzumab group). Scale bar is 5 mm. E, Representative image taken by x4 and x40 objectives using light microscopy, of one tumor from AG2G+trastuzumab group stained with anti-human CD3 antibody by IHC. F, Tumor volume measured by caliper in NSG mice treated with a single injection of T cells (10 7 ) 13 days after tumor inoculation (2.5×10 6 NCI-N87). Antibodies were injected once a week for a total of 3 times (250 μg/mouse), n = 5. G, NSG mice were injected with 1.7×10 6 NCI-N87 tumor cells, after tumor reached 100 to 200 mm 3 mice were randomized and treated once with intravenous injection of 5×10 6 AG2G-expressing T cells and with intraperitoneal injection of saline or 250-μg trastuzumab. On days 1, 7, 14, 28 following treatment, 3 mice from each group were sacrificed and RNA was extracted from blood and tumors for real-time PCR analysis of AG2G-expressing cells ( n = 12). Graphs show mean ± SD. Graphs A – C show one of two independent experiments performed. Statistical significance was calculated using two-way ANOVA with Tukey's correction for multiple comparisons for A and B , one-way ANOVA with Holm-Sidak correction for multiple comparisons for C . *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. Error bars represent standard error.

Article Snippet: For activation of effector cells on plastic-bound ligands, monoclonal antibodies or recombinant Human HER2/ErbB2 Protein (ECD) Biotinylated (Sino Biological, #10004-HCCH-B) were incubated on Maxisorp ELISA 96-well plates (Nunc Thermos Fisher Scientific) in PBS for 24 hours, washed three times and 10 5 T cells were incubated for 24 hours in 150 μL RPMI medium supplemented with 10% FBS.

Techniques: Expressing, In Vivo, Injection, Light Microscopy, Staining, Real-time Polymerase Chain Reaction

Journal: Cancer Immunology Research

Article Title: T Cells Expressing a Modified FcγRI Exert Antibody-Dependent Cytotoxicity and Overcome the Limitations of CAR T-cell Therapy against Solid Tumors

doi: 10.1158/2326-6066.CIR-22-0423

Figure Lengend Snippet: Retroviral transduction of γδ-T cells with AG2G endows them with antitumor ADCC. A, Flow cytometry analysis of sham or AG2G-transduced αβ-T cells (two left panels, activated with IL2 and anti-CD3); sham or AG2G-transduced γδ-T cells (two right panels, activated with IL2 and zoledronic acid). B, Live cell numbers of HT29-HER2 expressing cells, calculated by incuCyte software, cocultured with AG2G-expressing γδ-T cells or AG2G-expressing αβ-T cells, with or without trastuzumab ( n = 3). C, Live cell numbers of HT29-HER2 expressing cells, calculated by incuCyte software, cocultured with sham untransduced or AG2G-expressing γδ-T cells with or without trastuzumab, in E:T ratios of 1:1, 2:1 or 4:1 ( n = 4). D, ELISA measurement of IFNγ levels in supernatants of 48-hour sham or AG2G-expressing γδ-T cells cocultured with HT29-HER2 expressing cells, with or without trastuzumab (E:T ratio 4:1, n = 3). Graphs show mean ±SD. Statistical significance was calculated using two-way ANOVA with Tukey's correction for multiple comparisons for B and C . Two-way ANOVA with Sidak correction for multiple comparisons for D . *, P < 0.05; **, P < 0.01; ****, P < 0.0001. Error bars represent standard error.

Article Snippet: For activation of effector cells on plastic-bound ligands, monoclonal antibodies or recombinant Human HER2/ErbB2 Protein (ECD) Biotinylated (Sino Biological, #10004-HCCH-B) were incubated on Maxisorp ELISA 96-well plates (Nunc Thermos Fisher Scientific) in PBS for 24 hours, washed three times and 10 5 T cells were incubated for 24 hours in 150 μL RPMI medium supplemented with 10% FBS.

Techniques: Transduction, Flow Cytometry, Expressing, Software, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: A new portable toluidine blue/aptamer complex-on-polyethyleneimine-coated gold nanoparticles-based sensor for label-free electrochemical detection of alpha-fetoprotein

doi: 10.3389/fbioe.2023.1182880

Figure Lengend Snippet: (A) Reproducibility of the TB/BSA/Apt/PEI-AuNPs-based aptasensor, after binding with 5.0 ng mL −1 AFP, (B) interference study for the detection of 5.0 ng mL −1 AFP at the presence of interferences including AA, Glu, DA, IgG, GM2AP, IL-6, CEA, MMP-7, HER-2, mixture of interferences, and mixture of interferences without AFP, and (C) stability test for the aptasensors over 42 days.

Article Snippet: Recombinant human HER2/ErbB2 protein (HER2, lot: LC11MC0201) was bought from Sino Biological Inc.

Techniques: Binding Assay

Journal: Frontiers in Immunology

Article Title: Design and selection of optimal ErbB-targeting bispecific antibodies in pancreatic cancer

doi: 10.3389/fimmu.2023.1168444

Figure Lengend Snippet: Biochemical characterization of ErbB-specific BiXAb™. (A) Schematic illustration of the BiXAb™ platform. (B) Representative SDS-PAGE analysis of the BiXAb™ 1Cetu-2Pertu-Fc and 2Pertu-1Cetu-Fc in non-reducing and reducing conditions. Proteins were stained with Coomassie brilliant blue. IgG1 was used as control and molecular weight markers are indicated. Other BiXAb™ are in Supplementary Figure 1 . (C) Representative results of ELISA to assess binding of 1Cetu-2Pertu-Fc, 2Pertu-1Cetu-Fc, 1Cetu, and 2Pertu to immobilized EGFR (top panel) and HER2 (bottom panel). See Supplementary Figure 2 for all antibodies used in this study. (D) EC50 values extracted from ELISA binding curves.

Article Snippet: Tag-free recombinant human HER2 (SB #10004-HCCH) and HER3 (SB #10201-HCCH) ECD, and His-tagged recombinant human EGFR (SB #10001-H08H), HER2 (SB #10004-H08H) and HER3 (SB #10201-H08H) ECD were purchased from Sino Biological Inc (China).

Techniques: SDS Page, Staining, Molecular Weight, Enzyme-linked Immunosorbent Assay, Binding Assay

Journal: Frontiers in Immunology

Article Title: Design and selection of optimal ErbB-targeting bispecific antibodies in pancreatic cancer

doi: 10.3389/fimmu.2023.1168444

Figure Lengend Snippet: ELISA to assess the simultaneous binding of (A) 2Trastu-1Cetu-Fc to immobilized HER2 and EGFR-His (left) and immobilized EGFR and HER2-His (right); (B) 3Patri-2Trastu-Fc to immobilized HER3 and HER2-His (left) and immobilized HER2 and HER3-His (right); (C) 3Patri-1Cetu-Fc to immobilized HER3 and EGFR-His (left) and immobilized EGFR and HER3-His (right); and (D) 3Patri-1Matu-Fc to immobilized HER3 and EGFR-His (left) and immobilized EGFR and HER3-His (right). The EC50 values calculated from the ELISA binding curves are indicated.

Article Snippet: Tag-free recombinant human HER2 (SB #10004-HCCH) and HER3 (SB #10201-HCCH) ECD, and His-tagged recombinant human EGFR (SB #10001-H08H), HER2 (SB #10004-H08H) and HER3 (SB #10201-H08H) ECD were purchased from Sino Biological Inc (China).

Techniques: Enzyme-linked Immunosorbent Assay, Binding Assay

Journal: Frontiers in Immunology

Article Title: Design and selection of optimal ErbB-targeting bispecific antibodies in pancreatic cancer

doi: 10.3389/fimmu.2023.1168444

Figure Lengend Snippet: Comparison of the four lead BiXAb™ vs 2MAbs and single parental antibodies (1MAbs). (A) Effect on the phosphoproteome. Cells were pre-stimulated with BiXAb™, 2MAbs or 1MAbs for 20 min before adding a mixture of NRG1 and EGF for 10 min. Activation rate is relative to the maximal (100%) phosphorylation obtained in NRG1/EGFR-stimulated cells without antibodies. HuIgG1 IRR and HLA-DR-CD5-Fc were used as negative controls for 1MAbs and BiXAb™, respectively. The tyrosine kinase inhibitors iMEK and iPI3K were used for comparison. (B) Feedback loop strengths by modular response analysis in the indicated pancreatic cancer cell lines. (C) Effect on ErbB expression. The indicated cell lines were incubated with the indicated antibodies for 6 h. After cell lysis, EGFR, HER2 and HER3 levels were evaluated by sandwich ELISA (RD Systems) using receptor-specific antibodies. The percentage of expression was quantified relative to negative control (culture medium alone). The NRG1+EGF mixture was used as positive control. (D) Effect on ADCC assessed by monitoring CD107a degranulation and IFNγ secretion. The indicated cell lines were co-cultured with CD16-transfected NK92 cells and incubated with BiXAb™, 2MAbs or 1MAbs for 4 h. The percentage of effector NK cells positive for CD107a and/or IFNγ was measured by flow cytometry. Rituximab was used as irrelevant antibody. Data are the mean ± SEM from three replicates. The NRG1 + EGF mixture was used as negative control of ADCC. (E) Effect on cell viability. BxPC3 cells were incubated with BiXAb™, 2MAbs or 1MAbs for 5 days, and cell viability was measured using the MTS proliferation assay. (F) Effect on cell apoptosis. The indicated cells were incubated with BiXAb™ or 2MAbs for 48 h, and then labeled with fluorescein-conjugated Annexin V and 7-AminoActinomycin-D before apoptosis measurement (%) by flow cytometry. Medium and staurosporine (3 h incubation) were used as negative and positive controls of apoptosis, respectively. HLADR-CD5-Fc was used as irrelevant BiXAb™. Caspase 3/7 activation is reported in Supplementary Figure 5 .

Article Snippet: Tag-free recombinant human HER2 (SB #10004-HCCH) and HER3 (SB #10201-HCCH) ECD, and His-tagged recombinant human EGFR (SB #10001-H08H), HER2 (SB #10004-H08H) and HER3 (SB #10201-H08H) ECD were purchased from Sino Biological Inc (China).

Techniques: Activation Assay, Expressing, Incubation, Lysis, Sandwich ELISA, Negative Control, Positive Control, Cell Culture, Transfection, Flow Cytometry, Proliferation Assay, Labeling

Journal: Cell Metabolism

Article Title: The mitochondrial pyruvate carrier regulates memory T cell differentiation and antitumor function

doi: 10.1016/j.cmet.2022.03.013

Figure Lengend Snippet: MPC inhibition during CAR T cell in vitro activation and expansion induces superior antitumor activity upon ACT in a mouse melanoma model (A) Tumor growth following ACT of DMSO or UK5099 (MPCi)-conditioned OT1 T cells. (B and C) Number of transferred cells (B) and their percentage of T CM (C) in the spleen. (D–H) Number of tumor-infiltrating transferred cells per milligram of tumor (D) and their percentage of TCF1-positive cells (E), Tpex (F), Tex (G), and cells co-expressing PD1, LAG3, and TIM3 (H). (I) Number of tumor-infiltrating transferred cells co-expressing IFNγ and TNF. In (A)–(I), n = 11 mice (DMSO) and 14 mice (MPCi); pooled data from 2 independent experiments. (J and K) Tumor growth (J) and weight (K) of B16-HER2 tumors following treatment with DMSO or MPCi-conditioned HER2-CAR or BFP control T cells. (L and M) Percentage of T CM cells (L) and TCF1-expressing cells (M) out of HER2-CAR-positive cells in the blood 12 days after ACT. (N and O) Number of HER2-CAR-positive cells (N) and their percentage of T CM (O) in the tumor-draining lymph node. (P and Q) Number of HER2-CAR-positive cells (P) and their percentage of TCF1-positive cells (Q) in the spleen. (R–V) Number of tumor-infiltrating HER2-CAR-positive cells per milligram of tumor (R) and their percentage of TCF1-positive cells (S), Tpex (T), Tex (U), and cells co-expressing PD1 and TIM3 (V). In (J)–(V), n = 11 mice (untreated), 4–5 mice (DMSO- and MPCi-BFP-T ACT), 12–13 mice (DMSO- and MPCi-HER2-CAR T ACT). BFP data are derived from 1 experiment; untreated and HER2-CAR T cell transfer is pooled data from 2 independent experiments. Data are represented as mean ± SEM. Statistics are based on unpaired, two-tailed Student’s t test (A–I and L–V) or one-way ANOVA (J and K), ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, and ns (p > 0.05). See also Figure S6 .

Article Snippet: recombinant human Fc-tagged Her2/ErbB2 protein , Sino Biological , Cat# 10004-H02H.

Techniques: Inhibition, In Vitro, Activation Assay, Activity Assay, Expressing, Derivative Assay, Two Tailed Test

Journal: Cell Metabolism

Article Title: The mitochondrial pyruvate carrier regulates memory T cell differentiation and antitumor function

doi: 10.1016/j.cmet.2022.03.013

Figure Lengend Snippet:

Article Snippet: recombinant human Fc-tagged Her2/ErbB2 protein , Sino Biological , Cat# 10004-H02H.

Techniques: Staining, Recombinant, Expressing, Lysis, Selection, Purification, Plasmid Preparation, Bicinchoninic Acid Protein Assay, Magnetic Beads, Western Blot, Sequencing, Software