recombinant human egf  (PeproTech)

 
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    Name:
    Recombinant Human EGF
    Description:
    EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells Additionally EGF has been shown to inhibit gastric secretion and to be involved in wound healing EGF signals through a receptor known as c erbB which is a class I tyrosine kinase receptor This receptor also binds with TGF α and VGF vaccinia virus growth factor Recombinant human EGF is a 6 2 kDa globular protein containing 53 amino acid residues including 3 intramolecular disulfide bonds strong strong br p em strong Animal Free Human EGF Catalog Number AF 100 15 has replaced Human EGF Catalog Number 100 15 strong em strong strong p
    Catalog Number:
    AF-100-15-100UG
    Price:
    80.00
    Category:
    Recombinant Proteins
    Source:
    E.coli
    Reactivity:
    Cow Hamster Monkey Mouse Pig Rabbit Rat
    Purity:
    98.0
    Quantity:
    100UG
    Buy from Supplier


    Structured Review

    PeproTech recombinant human egf
    Average cumulative migration of keratinocytes on gradients of immobilized <t>SS-EGF,</t> <t>SS-IGF-1,</t> and SS-IGF-1+SS-EGF and TCPS over 7 days. (a) g2 (power law) gradient patterns of SS-EGF (150 μg/ml), SS-IGF-1 patterned on SS-EGF (each 75 μg/ml
    EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells Additionally EGF has been shown to inhibit gastric secretion and to be involved in wound healing EGF signals through a receptor known as c erbB which is a class I tyrosine kinase receptor This receptor also binds with TGF α and VGF vaccinia virus growth factor Recombinant human EGF is a 6 2 kDa globular protein containing 53 amino acid residues including 3 intramolecular disulfide bonds strong strong br p em strong Animal Free Human EGF Catalog Number AF 100 15 has replaced Human EGF Catalog Number 100 15 strong em strong strong p
    https://www.bioz.com/result/recombinant human egf/product/PeproTech
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant human egf - by Bioz Stars, 2021-04
    99/100 stars

    Images

    1) Product Images from "Co-immobilization of gradient-patterned growth factors for directed cell migration"

    Article Title: Co-immobilization of gradient-patterned growth factors for directed cell migration

    Journal:

    doi: 10.1007/s10439-008-9581-1

    Average cumulative migration of keratinocytes on gradients of immobilized SS-EGF, SS-IGF-1, and SS-IGF-1+SS-EGF and TCPS over 7 days. (a) g2 (power law) gradient patterns of SS-EGF (150 μg/ml), SS-IGF-1 patterned on SS-EGF (each 75 μg/ml
    Figure Legend Snippet: Average cumulative migration of keratinocytes on gradients of immobilized SS-EGF, SS-IGF-1, and SS-IGF-1+SS-EGF and TCPS over 7 days. (a) g2 (power law) gradient patterns of SS-EGF (150 μg/ml), SS-IGF-1 patterned on SS-EGF (each 75 μg/ml

    Techniques Used: Migration

    2) Product Images from "Alix is required during development for normal growth of the mouse brain"

    Article Title: Alix is required during development for normal growth of the mouse brain

    Journal: Scientific Reports

    doi: 10.1038/srep44767

    Alix ko NPCs in culture do not undergo abnormal apoptosis but are deficient in endocytosis and FGF signaling. ( a ) NPCs were dissociated from E12.5 embryos, cultured for 3 DIV and apoptotic cells immunostained for C3a and BrdU revealing no difference in proliferation or apoposis (n = 3 embryos). ( b ) Alix ko NPCs form fewer neurospheres in culture. Cells dissociated from E12.5 embryos were grown for 6 days on a non permissive substrate in presence of FGF or EGF and FGF. Graphs show the number of neurospheres formed from 1000 seeded cells prepared from embryos of each genotype (n = 6 for wt and n = 5 for Alix ko *p
    Figure Legend Snippet: Alix ko NPCs in culture do not undergo abnormal apoptosis but are deficient in endocytosis and FGF signaling. ( a ) NPCs were dissociated from E12.5 embryos, cultured for 3 DIV and apoptotic cells immunostained for C3a and BrdU revealing no difference in proliferation or apoposis (n = 3 embryos). ( b ) Alix ko NPCs form fewer neurospheres in culture. Cells dissociated from E12.5 embryos were grown for 6 days on a non permissive substrate in presence of FGF or EGF and FGF. Graphs show the number of neurospheres formed from 1000 seeded cells prepared from embryos of each genotype (n = 6 for wt and n = 5 for Alix ko *p

    Techniques Used: Cell Culture

    3) Product Images from "A Sema3C Mutant Resistant to Cleavage by Furin (FR-Sema3C) Inhibits Choroidal Neovascularization"

    Article Title: A Sema3C Mutant Resistant to Cleavage by Furin (FR-Sema3C) Inhibits Choroidal Neovascularization

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0168122

    FR-sema3C inhibits VEGF and PDGF-BB induced phosphorylation of ERK1/2 in endothelial cells. (A) HUVEC were stimulated or not with VEGF (30 ng/ml) in the presence of elution buffer (100 mM glycine, 24 mM Tris, pH-7.2) or FR-sema3C/Fc (2 μg/ml). After 10 min. at room temperature the cells were lysed and ERK1/2 phosphorylation was determined by western blot as described. Shown is a representative experiment out of three that gave similar results. Below is shown a histogram depicting the ratio between the intensity of the respective phospho-ERK1/2 bands and the total ERK bands. (B) HUVEC were stimulated with PDGF-BB (50 ng/ml) in the presence or absence of FR-sema3C/Fc (2 μg/ml). ERK1/2 phosphorylation was determined and quantified as described under A. Shown is a representative experiment out of three that gave similar results. (C) HUVEC were stimulated with bFGF (5 ng/ml) in the presence or absence of FR-sema3C/Fc (2 μg/ml). ERK1/2 phosphorylation was determined and quantified as described under A. Shown is a representative experiment out of three that gave similar results. (D) HUVEC were stimulated with HGF (50 ng/ml) in the presence or absence of FR-sema3C/Fc (2 μg/ml). ERK1/2 phosphorylation was determined and quantified as described under A. Shown is a representative experiment out of three that gave similar results. (E) HUVEC were stimulated with EGF (50 ng/ml) in the presence or absence of FR-sema3C/Fc (2 μg/ml). ERK1/2 phosphorylation was determined and quantified as described under A. Shown is a representative experiment out of three that gave similar results. Statistical significance was evaluated using the one tailed Mann-Whitney test. Error bars represent the standard error of the mean. *: p
    Figure Legend Snippet: FR-sema3C inhibits VEGF and PDGF-BB induced phosphorylation of ERK1/2 in endothelial cells. (A) HUVEC were stimulated or not with VEGF (30 ng/ml) in the presence of elution buffer (100 mM glycine, 24 mM Tris, pH-7.2) or FR-sema3C/Fc (2 μg/ml). After 10 min. at room temperature the cells were lysed and ERK1/2 phosphorylation was determined by western blot as described. Shown is a representative experiment out of three that gave similar results. Below is shown a histogram depicting the ratio between the intensity of the respective phospho-ERK1/2 bands and the total ERK bands. (B) HUVEC were stimulated with PDGF-BB (50 ng/ml) in the presence or absence of FR-sema3C/Fc (2 μg/ml). ERK1/2 phosphorylation was determined and quantified as described under A. Shown is a representative experiment out of three that gave similar results. (C) HUVEC were stimulated with bFGF (5 ng/ml) in the presence or absence of FR-sema3C/Fc (2 μg/ml). ERK1/2 phosphorylation was determined and quantified as described under A. Shown is a representative experiment out of three that gave similar results. (D) HUVEC were stimulated with HGF (50 ng/ml) in the presence or absence of FR-sema3C/Fc (2 μg/ml). ERK1/2 phosphorylation was determined and quantified as described under A. Shown is a representative experiment out of three that gave similar results. (E) HUVEC were stimulated with EGF (50 ng/ml) in the presence or absence of FR-sema3C/Fc (2 μg/ml). ERK1/2 phosphorylation was determined and quantified as described under A. Shown is a representative experiment out of three that gave similar results. Statistical significance was evaluated using the one tailed Mann-Whitney test. Error bars represent the standard error of the mean. *: p

    Techniques Used: Western Blot, One-tailed Test, MANN-WHITNEY

    Related Articles

    Recombinant:

    Article Title: A Sema3C Mutant Resistant to Cleavage by Furin (FR-Sema3C) Inhibits Choroidal Neovascularization
    Article Snippet: Materials Recombinant VEGF165 and bFGF were produced and purified as previously described [ , ]. .. Recombinant PDGF-BB, recombinant HGF and recombinant EGF were purchased (Recombinant human PDGF-BB, recombinant human HGF and recombinant human EGF, PeproTech, Rocky Hill, New Jersey, USA). .. Human FR-sema3C was produced as previously described [ ].

    Article Title: The microRNA cluster miR-106b~25 regulates adult neural stem/progenitor cell proliferation and neuronal differentiation
    Article Snippet: NSPCs were purified from myelin with a 22.5% Percoll gradient (GE Healthcare) and then from red blood cells with a 58.5% Percoll gradient. .. Freshly isolated NSPCs were considered “passage 1.” NSPCs were grown at 5% CO2 in a 37°C incubator at 50,000 cells/ml in Neurobasal A Medium (NBA; Invitrogen) supplemented with 1X PSQ, 1X B-27 Supplement Minus Vitamin A (B27; Invitrogen), 20 ng/ml recombinant human bFGF (PeproTech), and 20 ng/ml recombinant human EGF (PeproTech). ..

    Article Title: Neural Precursor Cell Proliferation Is Disrupted Through Activation of the Aryl Hydrocarbon Receptor by 2,3,7,8-Tetrachlorodibenzo-p-Dioxin
    Article Snippet: Human plasma fibronectin was obtained from Millipore. .. Recombinant human epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) were obtained from Peprotech. ..

    Article Title: Signaling Network State Predicts Twist-Mediated Effects on Breast Cell Migration Across Diverse Growth Factor Contexts *
    Article Snippet: Antibodies against E-cadherin, N-cadherin, and vimentin, as well as human recombinant IGF-1, HGF, and PDGF-BB were purchased from BD Biosciences (San Jose, CA). .. Human recombinant EGF and HRG-β1 were purchased from Peprotech (Rocky Hill, NJ) and Sigma-Aldrich (St. Louis, MO), respectively. .. Cell Culture Immortalized human mammary epithelial cells (hMLEs) expressing either the empty pBabe puro vector (pBp) or pBP-Twist1 (referred to as epithelial or mesenchymal hMLEs) were obtained from the Robert Weinberg laboratory at the Whitehead Institute for Biomedical Research (Cambridge, MA) and cultured as described previously ( ) with complete medium consisting of 50% mammary epithelial basal medium (MEBM) (Lonza; Walkersville, MD), 25% Ham's F-12, 25% Dulbecco's modified Eagle's medium (Invitrogen; Carlsbad, CA) and an MEGM Bulletkit (Lonza) containing bovine pituitary extract, EGF, insulin, hydrocortisone, and gentamycin.

    Article Title: EGF-mediated EGFR/ERK signaling pathway promotes germinative cell proliferation in Echinococcus multilocularis that contributes to larval growth and development
    Article Snippet: .. Metacestode vesicles were cultivated in conditioned medium (Control) supplemented with 1–100 ng/mL recombinant human EGF for 49 days. ..

    Article Title: Protein phosphatase 2Cδ/Wip1 regulates phospho-p90RSK2 activity in lesional psoriatic skin
    Article Snippet: Second-passage keratinocytes were trypsinated and seeded in 10 cm petri dishes, 4.5×106 cells/plate in keratinocyte basal medium (Gibco 17005; Thermo Fisher Scientific, Waltham, MA, USA), with only bovine pituitary growth hormone added from the supplement Gibco 37000-015, 50 µg/mL), gentamicin (Gibco 15710, 5 µg/mL), and 2% fetal calf serum (FCS; Gibco 160000 44). .. After 24 hours, the cells were either left alone or preincubated with DMF (140 µM) or 50 µM of PD98059 for 1 hour and stimulated with recombinant MIF (100 ng/mL; R & D Systems, Oxon, UK) or h-EGF (2 ng/mL; PeproTech, London, UK) for 24 hours. .. Alternatively, the cells were stimulated with MIF for 0 and 30 minutes and 2, 12, 24, 48, and 96 hours alone or after preincubation with 140 µM DMF (47967-25G-F; Sigma-Aldrich, Brøndby, Denmark).

    Article Title: A combinatorial strategy for treating KRAS mutant lung cancer
    Article Snippet: Plasmids and recombinant proteins All vectors were derived from the Murine Stem Cell Virus (MSCV, Clontech) retroviral vector backbone. miR30- and mirE-based shRNAs were designed and cloned as previously described and sequences are available in . shRNAs were cloned into the TRMPV-Neo (pSIN-TREdsRed-miR30-PGK-Venus-IRES-NeoR), LT3GEPIR (TRE3G-GFP-miRE-PGK-PuroR-IRES-rtTA3), and MLP (LTR-miR30-PGK-PuroR-IRES-GFP) vectors as previously described . .. Recombinant proteins FGF2 (8910, Cell Signaling), HGF (100-39, Peprotech), EGF (AF-100-15, Peprotech), and NRG1 (100-03, Peprotech) were used at 50 ngml−1 for 10 minutes. .. Cell culture, compounds, and competitive proliferation assays H23, H460, H2030, H358, H2122, H820, H3255, and A549 cells were kindly provided by R. Somwar and H. Varmus (Cornell University, New York).

    Isolation:

    Article Title: The microRNA cluster miR-106b~25 regulates adult neural stem/progenitor cell proliferation and neuronal differentiation
    Article Snippet: NSPCs were purified from myelin with a 22.5% Percoll gradient (GE Healthcare) and then from red blood cells with a 58.5% Percoll gradient. .. Freshly isolated NSPCs were considered “passage 1.” NSPCs were grown at 5% CO2 in a 37°C incubator at 50,000 cells/ml in Neurobasal A Medium (NBA; Invitrogen) supplemented with 1X PSQ, 1X B-27 Supplement Minus Vitamin A (B27; Invitrogen), 20 ng/ml recombinant human bFGF (PeproTech), and 20 ng/ml recombinant human EGF (PeproTech). ..

    MTT Assay:

    Article Title: Cytohesins/ARNO: The Function in Colorectal Cancer Cells
    Article Snippet: The rabbit or mouse monoclonal anti-human antibodies used were ARNO (Abcam, ab56510), pEGFR (Py1068, Epitomics, 1138-1), pERK1/2 (T202/Y204, Bioworld, BS5016), EGFR (Cell Signaling, 3197), GAPDH (Bioworld, AP0063), IGF-IR (Abcam, ab39675), pIGF-IR (Abcam, ab39398), pIRS (Abcam, ab52167), pAKT (Abcam, ab106693), pIRS1 (Abcam, ab66153), pShc (Abcam, ab155170), and Ki-67(Cell Signaling, 9027). .. Other reagents and equipment used were as follows: SecinH3 (Merck-565725/sc-203260), siRNA oligo (Genephama), MTT (sigma, m5655), DMSO (sigma, D5879), human EGF (Peprotech, AF-100-15), human IGF-1 (Peprotech, AF-100-11), FBS (Gibco, USA), and 0.25% trypsin (Sigma), immunohistochemical kit(Zhongshan,China). .. Cell lines and cultivation The human colorectal cancer cell lines HT29 and HCT116, which were identified without any mutation in KRAS and BRAF, were obtained from the Key Laboratory of Cancer Prevention and Intervention, Cancer Institute, Second Affiliated Hospital, School of Medicine, Zhejiang University, China.

    Immunohistochemistry:

    Article Title: Cytohesins/ARNO: The Function in Colorectal Cancer Cells
    Article Snippet: The rabbit or mouse monoclonal anti-human antibodies used were ARNO (Abcam, ab56510), pEGFR (Py1068, Epitomics, 1138-1), pERK1/2 (T202/Y204, Bioworld, BS5016), EGFR (Cell Signaling, 3197), GAPDH (Bioworld, AP0063), IGF-IR (Abcam, ab39675), pIGF-IR (Abcam, ab39398), pIRS (Abcam, ab52167), pAKT (Abcam, ab106693), pIRS1 (Abcam, ab66153), pShc (Abcam, ab155170), and Ki-67(Cell Signaling, 9027). .. Other reagents and equipment used were as follows: SecinH3 (Merck-565725/sc-203260), siRNA oligo (Genephama), MTT (sigma, m5655), DMSO (sigma, D5879), human EGF (Peprotech, AF-100-15), human IGF-1 (Peprotech, AF-100-11), FBS (Gibco, USA), and 0.25% trypsin (Sigma), immunohistochemical kit(Zhongshan,China). .. Cell lines and cultivation The human colorectal cancer cell lines HT29 and HCT116, which were identified without any mutation in KRAS and BRAF, were obtained from the Key Laboratory of Cancer Prevention and Intervention, Cancer Institute, Second Affiliated Hospital, School of Medicine, Zhejiang University, China.

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  • 99
    PeproTech h egf
    Schematic representations of <t>EGF-</t> and <t>MIF-induced</t> activation of P-ERK1, 2 and the phosphorylation of MSK1/2 and RSK1/2 kinases. P-MSK1/2 induces P-NF-κB/p65 (S276) and the transcription of PP2Cδ/Wip1, which is inhibited by DMF; 24 hours stimulation by EGF and MIF induces P-Wip1/Wip1, resulting in dephosphorylation of P-RSK2 (S386) in complex formation with P-RSK2. This is inhibited by costimulation with the ERK1/2 inhibitor PD98059 or DMF. Abbreviations: DMF, dimethyl fumarate; EGF, epidermal growth factor; ERK, extracellular signal-regulated kinase; MIF, macrophage migration inhibitory factor; MSK, mitogen- and stress-activated kinase; NF-κB, nuclear factor-κB; PP2Cδ, protein phosphatase 2Cδ; RSK, p90 ribosomal S6 kinase; Wip1, wild-type p53-induced phosphatase 1.
    H Egf, supplied by PeproTech, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/h egf/product/PeproTech
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    h egf - by Bioz Stars, 2021-04
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    98
    PeproTech mouse egf
    Cell migration through the <t>microchannels.</t> (A), (B), (C) and (D) show cell migration in the channels containing <t>EGF+ve,</t> with EGF-ve, EGF+Anti-Apt and EGF+Mut-Apt; cells in all groups were cultured at 37 °C for 96 h. (E) depicts the number of channels
    Mouse Egf, supplied by PeproTech, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse egf/product/PeproTech
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse egf - by Bioz Stars, 2021-04
    98/100 stars
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    Image Search Results


    Schematic representations of EGF- and MIF-induced activation of P-ERK1, 2 and the phosphorylation of MSK1/2 and RSK1/2 kinases. P-MSK1/2 induces P-NF-κB/p65 (S276) and the transcription of PP2Cδ/Wip1, which is inhibited by DMF; 24 hours stimulation by EGF and MIF induces P-Wip1/Wip1, resulting in dephosphorylation of P-RSK2 (S386) in complex formation with P-RSK2. This is inhibited by costimulation with the ERK1/2 inhibitor PD98059 or DMF. Abbreviations: DMF, dimethyl fumarate; EGF, epidermal growth factor; ERK, extracellular signal-regulated kinase; MIF, macrophage migration inhibitory factor; MSK, mitogen- and stress-activated kinase; NF-κB, nuclear factor-κB; PP2Cδ, protein phosphatase 2Cδ; RSK, p90 ribosomal S6 kinase; Wip1, wild-type p53-induced phosphatase 1.

    Journal: Journal of Inflammation Research

    Article Title: Protein phosphatase 2Cδ/Wip1 regulates phospho-p90RSK2 activity in lesional psoriatic skin

    doi: 10.2147/JIR.S152869

    Figure Lengend Snippet: Schematic representations of EGF- and MIF-induced activation of P-ERK1, 2 and the phosphorylation of MSK1/2 and RSK1/2 kinases. P-MSK1/2 induces P-NF-κB/p65 (S276) and the transcription of PP2Cδ/Wip1, which is inhibited by DMF; 24 hours stimulation by EGF and MIF induces P-Wip1/Wip1, resulting in dephosphorylation of P-RSK2 (S386) in complex formation with P-RSK2. This is inhibited by costimulation with the ERK1/2 inhibitor PD98059 or DMF. Abbreviations: DMF, dimethyl fumarate; EGF, epidermal growth factor; ERK, extracellular signal-regulated kinase; MIF, macrophage migration inhibitory factor; MSK, mitogen- and stress-activated kinase; NF-κB, nuclear factor-κB; PP2Cδ, protein phosphatase 2Cδ; RSK, p90 ribosomal S6 kinase; Wip1, wild-type p53-induced phosphatase 1.

    Article Snippet: After 24 hours, the cells were either left alone or preincubated with DMF (140 µM) or 50 µM of PD98059 for 1 hour and stimulated with recombinant MIF (100 ng/mL; R & D Systems, Oxon, UK) or h-EGF (2 ng/mL; PeproTech, London, UK) for 24 hours.

    Techniques: Activation Assay, De-Phosphorylation Assay, Migration

    ( A ) Complex formation of Wip1 with RSK2 in human keratinocytes was induced by EGF and MIF. Keratinocytes were either left alone or preincubated with DMF (140 µM) or PD98059 (50 µM) for 1 hour and stimulated with EGF (2 ng/mL) or MIF (100 ng/mL) for 24 hours. Whole cell extracts were immunoprecipitated with anti-RSK2 antibody and analyzed by WB with anti-Wip1 (H-300) (sc-20712 epitope C-terminus) and anti-RSK2; 1 representative gel is shown. ( B ) Densitometry of 6 different experiments from pooled data shows the ratio of P-Wip1/Wip1 (both) associated with RSK2. EGF-stimulated versus (co) untreated cells * p =0.003; MIF stimulated versus (co) untreated cells ¤ p =0.037; EGF-stimulated versus (DMF and EGF) ** p =0.008; MIF-stimulated versus (DMF and MIF) p =0.059; EGF-stimulated versus (PD98059 and EGF) *** p

    Journal: Journal of Inflammation Research

    Article Title: Protein phosphatase 2Cδ/Wip1 regulates phospho-p90RSK2 activity in lesional psoriatic skin

    doi: 10.2147/JIR.S152869

    Figure Lengend Snippet: ( A ) Complex formation of Wip1 with RSK2 in human keratinocytes was induced by EGF and MIF. Keratinocytes were either left alone or preincubated with DMF (140 µM) or PD98059 (50 µM) for 1 hour and stimulated with EGF (2 ng/mL) or MIF (100 ng/mL) for 24 hours. Whole cell extracts were immunoprecipitated with anti-RSK2 antibody and analyzed by WB with anti-Wip1 (H-300) (sc-20712 epitope C-terminus) and anti-RSK2; 1 representative gel is shown. ( B ) Densitometry of 6 different experiments from pooled data shows the ratio of P-Wip1/Wip1 (both) associated with RSK2. EGF-stimulated versus (co) untreated cells * p =0.003; MIF stimulated versus (co) untreated cells ¤ p =0.037; EGF-stimulated versus (DMF and EGF) ** p =0.008; MIF-stimulated versus (DMF and MIF) p =0.059; EGF-stimulated versus (PD98059 and EGF) *** p

    Article Snippet: After 24 hours, the cells were either left alone or preincubated with DMF (140 µM) or 50 µM of PD98059 for 1 hour and stimulated with recombinant MIF (100 ng/mL; R & D Systems, Oxon, UK) or h-EGF (2 ng/mL; PeproTech, London, UK) for 24 hours.

    Techniques: Immunoprecipitation, Western Blot

    ARNO enhanced the activation of EGFR. (a and b) SecinH3 and ARNO-siRNA reduced EGFR receptor signaling. Western blot analysis of HT29 cells treated with SecinH3 (a) or ARNO-siRNA (b) and stimulated with EGF is shown. Phosphorylation of the indicated proteins was determined by immunodetection using phosphospecific antibodies. Glyceraldehydes phosphate dehydrogenase (GAPDH) served as a loading control. The diagrams show relative phosphorylation levels after normalization for GAPDH. The untreated ligand-stimulated cells were set as 3 ( n = 3). Data is represented as the mean ± SEM. * p

    Journal: PLoS ONE

    Article Title: Cytohesins/ARNO: The Function in Colorectal Cancer Cells

    doi: 10.1371/journal.pone.0090997

    Figure Lengend Snippet: ARNO enhanced the activation of EGFR. (a and b) SecinH3 and ARNO-siRNA reduced EGFR receptor signaling. Western blot analysis of HT29 cells treated with SecinH3 (a) or ARNO-siRNA (b) and stimulated with EGF is shown. Phosphorylation of the indicated proteins was determined by immunodetection using phosphospecific antibodies. Glyceraldehydes phosphate dehydrogenase (GAPDH) served as a loading control. The diagrams show relative phosphorylation levels after normalization for GAPDH. The untreated ligand-stimulated cells were set as 3 ( n = 3). Data is represented as the mean ± SEM. * p

    Article Snippet: Other reagents and equipment used were as follows: SecinH3 (Merck-565725/sc-203260), siRNA oligo (Genephama), MTT (sigma, m5655), DMSO (sigma, D5879), human EGF (Peprotech, AF-100-15), human IGF-1 (Peprotech, AF-100-11), FBS (Gibco, USA), and 0.25% trypsin (Sigma), immunohistochemical kit(Zhongshan,China).

    Techniques: Activation Assay, Western Blot, Immunodetection

    The miR-106b ~ 25 cluster is expressed in adult NSPCs in culture. ( A ) Genomic locus of the mouse miR-106b~25 cluster and its host gene, Mcm7. ( B ) NSPCs (age 12 weeks, passage 2) were grown in multi-lineage differentiation conditions (no EGF or bFGF, with 1% FBS) for 7 days and then stained for Tuj1 (a marker of neurons), GFAP (a marker of astrocytes), or O4 (a marker of oligodendrocytes). Scale bar: 100 μm. ( C ) miRNA expression was determined by RT-qPCR in NSPCs in self-renewal conditions (with EGF and bFGF, no FBS) or differentiation conditions (no EGF or bFGF, with 1% FBS) for 4 days. Mean and SEM of gene expression relative to self-renewal conditions for 3 independent NSPC cultures (age 12 weeks, passage 2) are shown. One-sample two-tailed t-test, *: p

    Journal: Aging (Albany NY)

    Article Title: The microRNA cluster miR-106b~25 regulates adult neural stem/progenitor cell proliferation and neuronal differentiation

    doi:

    Figure Lengend Snippet: The miR-106b ~ 25 cluster is expressed in adult NSPCs in culture. ( A ) Genomic locus of the mouse miR-106b~25 cluster and its host gene, Mcm7. ( B ) NSPCs (age 12 weeks, passage 2) were grown in multi-lineage differentiation conditions (no EGF or bFGF, with 1% FBS) for 7 days and then stained for Tuj1 (a marker of neurons), GFAP (a marker of astrocytes), or O4 (a marker of oligodendrocytes). Scale bar: 100 μm. ( C ) miRNA expression was determined by RT-qPCR in NSPCs in self-renewal conditions (with EGF and bFGF, no FBS) or differentiation conditions (no EGF or bFGF, with 1% FBS) for 4 days. Mean and SEM of gene expression relative to self-renewal conditions for 3 independent NSPC cultures (age 12 weeks, passage 2) are shown. One-sample two-tailed t-test, *: p

    Article Snippet: Freshly isolated NSPCs were considered “passage 1.” NSPCs were grown at 5% CO2 in a 37°C incubator at 50,000 cells/ml in Neurobasal A Medium (NBA; Invitrogen) supplemented with 1X PSQ, 1X B-27 Supplement Minus Vitamin A (B27; Invitrogen), 20 ng/ml recombinant human bFGF (PeproTech), and 20 ng/ml recombinant human EGF (PeproTech).

    Techniques: Staining, Marker, Expressing, Quantitative RT-PCR, Two Tailed Test

    Cell migration through the microchannels. (A), (B), (C) and (D) show cell migration in the channels containing EGF+ve, with EGF-ve, EGF+Anti-Apt and EGF+Mut-Apt; cells in all groups were cultured at 37 °C for 96 h. (E) depicts the number of channels

    Journal: Biomedical microdevices

    Article Title: Proliferation and Migration of Tumor Cells in Tapered Channels

    doi: 10.1007/s10544-012-9721-0

    Figure Lengend Snippet: Cell migration through the microchannels. (A), (B), (C) and (D) show cell migration in the channels containing EGF+ve, with EGF-ve, EGF+Anti-Apt and EGF+Mut-Apt; cells in all groups were cultured at 37 °C for 96 h. (E) depicts the number of channels

    Article Snippet: Cells were seeded on the proximal side of the microchannels and were cultured with 20 ng/ml mouse EGF until cells migrated into at least 10 channels which took around 48 hours.

    Techniques: Migration, Cell Culture

    Cell migration through the microchannels. Cells were cultured at 37 °C for 24 to 96 h. (A), (B), (C) and (D) show cells spent different times to pass through the microchannels with EGF+ve, with EGF-ve, EGF+Anti-Apt and EGF+Mut-Apt respectively.

    Journal: Biomedical microdevices

    Article Title: Proliferation and Migration of Tumor Cells in Tapered Channels

    doi: 10.1007/s10544-012-9721-0

    Figure Lengend Snippet: Cell migration through the microchannels. Cells were cultured at 37 °C for 24 to 96 h. (A), (B), (C) and (D) show cells spent different times to pass through the microchannels with EGF+ve, with EGF-ve, EGF+Anti-Apt and EGF+Mut-Apt respectively.

    Article Snippet: Cells were seeded on the proximal side of the microchannels and were cultured with 20 ng/ml mouse EGF until cells migrated into at least 10 channels which took around 48 hours.

    Techniques: Migration, Cell Culture