recombinant haemagglutinin  (Sino Biological)


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    Name:
    Influenza A H5N1 Hemagglutinin HA Protein
    Description:
    A DNA sequence encoding the extracellular domain of hemagglutinin Influenza A virus A Viet Nam 1203 2004 H5N1 AAW80717 1 Met 1 Gln 531 with cleavage site mutated RERRRKKR→TETR HA1 HA2 uncleaved was fused with the C terminal polyhistidine tagged Fc region of mouse IgG1 at the C terminus
    Catalog Number:
    10003-V06H3
    Price:
    None
    Category:
    recombinant protein
    Host:
    HEK293 Cells
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    Structured Review

    Sino Biological recombinant haemagglutinin
    A DNA sequence encoding the extracellular domain of hemagglutinin Influenza A virus A Viet Nam 1203 2004 H5N1 AAW80717 1 Met 1 Gln 531 with cleavage site mutated RERRRKKR→TETR HA1 HA2 uncleaved was fused with the C terminal polyhistidine tagged Fc region of mouse IgG1 at the C terminus
    https://www.bioz.com/result/recombinant haemagglutinin/product/Sino Biological
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant haemagglutinin - by Bioz Stars, 2021-06
    95/100 stars

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    Related Articles

    Recombinant:

    Article Title: A Homogenous Fluorescence Quenching Based Assay for Specific and Sensitive Detection of Influenza Virus A Hemagglutinin Antigen
    Article Snippet: ReagentsCarboxyl quantum dots (QD655) were obtained from Life Technologies Inc. (Carlsbad, CA, USA). .. Recombinant influenza A virus H1N1 (A/New Caledonia/20/1999) HA, recombinant influenza A virus H5N1 (A/Viet Nam/1194/2004) HA, mouse monoclonal antibody to influenza A virus H1N1 HA (mAb-H1-HA), and mouse monoclonal antibody to influenza A virus H5N1 HA (mAb-H5-HA) were purchased from Sino Biological Inc. (Beijing, China). .. Two glycan, 6'-sialyllactose sodium salt (hereafter denoted as 6G) and 3'-sialyllactose sodium salt (hereafter denoted as 3G), were ordered from Carbosynth Limited Inc. (Berkshire, UK).

    Article Title: Potent influenza A virus entry inhibitors targeting a conserved region of hemagglutinin.
    Article Snippet: Influenza A viruses (IAVs) induce acute respiratory disease and cause significant morbidity and mortality throughout the world. .. Influenza A viruses (IAVs) induce acute respiratory disease and cause significant morbidity and mortality throughout the world. .. Influenza A viruses (IAVs) induce acute respiratory disease and cause significant morbidity and mortality throughout the world.

    Article Title: An oligothiophene compound neutralized influenza A viruses by interfering with hemagglutinin.
    Article Snippet: After incubation at 37 °C for 30 min, the fluorescence intensity was measured at an excitation wavelength of 340 nm and an emission wavelength of 535 nm using a microplate reader (Genios Pro, Tecan, US). .. Surface plasmon resonance (SPR)The HA of influenza A/Thailand/Kan353/2004(H5N1) and the HA of A/Vietnam/1194/2004(H5N1) were 99.82% identical, so we used HA and HA2 recombinant protein of influenza A/Vietnam/1194/2004(H5N1) bought from Sino Biological Inc. in this experiment. .. Compounds CL-385319, 4sc were fixed on the chip by photo-crosslinking, then recombinant HA (11062-V08H2-50, Sino Biological Inc. Beijing) or HA2 subuint protein (10003-V04H2-50, Sino Biological Inc. Beijing) at concentrations of 100 nM, 200 nM, 400 nM, 800 nM, 1600 nM were injected sequentially into the chamber in buffer PBST (0.05% Tween20, pH 4.5), the interaction of HA2 with the small molecules fixed was detected by PlexArrayTM HT SPRi (Seattle, US).

    Article Title: An angular fluidic channel for prism-free surface-plasmon-assisted fluorescence capturing.
    Article Snippet: .. Similarly, another influenza viral sample of H3N2 (A/Brisbane/10/2007; Prospec-Tany TechnoGene Ltd., Israel) and recombinant haemagglutinin from influenza H5N1 (A/chicken/ India/NIV33487/2006; Sino Biological Inc., China) and H1N1 (A/California/07/ 2009; Sino Biological Inc., China) with 2,000HA unitsml 1 were tested against the anti-A/Udorn/307/1972 antibody. ..

    Synthesized:

    Article Title: A novel neutralizing antibody against diverse clades of H5N1 influenza virus and its mutants capable of airborne transmission.
    Article Snippet: Highly pathogenic avian influenza A virus H5N1 continues to spread among poultry and has frequently broken the species barrier to humans. .. Highly pathogenic avian influenza A virus H5N1 continues to spread among poultry and has frequently broken the species barrier to humans. ..

    SPR Assay:

    Article Title: An oligothiophene compound neutralized influenza A viruses by interfering with hemagglutinin.
    Article Snippet: After incubation at 37 °C for 30 min, the fluorescence intensity was measured at an excitation wavelength of 340 nm and an emission wavelength of 535 nm using a microplate reader (Genios Pro, Tecan, US). .. Surface plasmon resonance (SPR)The HA of influenza A/Thailand/Kan353/2004(H5N1) and the HA of A/Vietnam/1194/2004(H5N1) were 99.82% identical, so we used HA and HA2 recombinant protein of influenza A/Vietnam/1194/2004(H5N1) bought from Sino Biological Inc. in this experiment. .. Compounds CL-385319, 4sc were fixed on the chip by photo-crosslinking, then recombinant HA (11062-V08H2-50, Sino Biological Inc. Beijing) or HA2 subuint protein (10003-V04H2-50, Sino Biological Inc. Beijing) at concentrations of 100 nM, 200 nM, 400 nM, 800 nM, 1600 nM were injected sequentially into the chamber in buffer PBST (0.05% Tween20, pH 4.5), the interaction of HA2 with the small molecules fixed was detected by PlexArrayTM HT SPRi (Seattle, US).

    Article Title: Synthesis of Four Pentacyclic Triterpene–Sialylglycopeptide Conjugates and Their Affinity Assays with Hemagglutinin
    Article Snippet: C135 H217 N11 O74 , ESI-MS (negative mode, m/z ): 1588.2 [M − 2H]2− , 1058.5 [M − 3H]3− ; HR-ESI-MS (positive mode, m/z ) 1589.1852 [M + 2H]2+ (calcd 1589.1850); 1 H-NMR (600 MHz, CD3 OD) δ 5.61 (s, 1H, CH-12), 5.13 (s, 1H, H-1 of Man-4), 4.34–4.33 (m, 2H, H-1 of Gal-6,Gal-6’), 3.00 (m, 2H, β-CH2 of Asn), 2.60 (m, 2H, H-3eq of NeuAc, NeuAc’), 1.42 (s, 3H, CH3 -27), 1.16 (s, 3H, CH3 -29), 1.14 (s, 6H, CH3 -26, 25), 0.99 (s, 3H, CH3 -23), 0.83 (s, 3H, CH3 -24), 0.80 (s, 3H, CH3-28). .. SPR Assay Biacore T200 instruments (GE Healthcare) were used to evaluate the binding affinity of compounds with H1N1 (A/WSN/1933) and H5N1 (A/Hong Kong/483/97), purchased from Sino Biological Inc. (Beijing, China) through SPR, as previously described [ ]. .. In brief, HA protein was immobilized on the surface of a CM5 chip by using the amine coupling approach at a flow rate of 10 μL/min in 10 mM sodium acetate buffer (pH 5.0).

    Binding Assay:

    Article Title: Synthesis of Four Pentacyclic Triterpene–Sialylglycopeptide Conjugates and Their Affinity Assays with Hemagglutinin
    Article Snippet: C135 H217 N11 O74 , ESI-MS (negative mode, m/z ): 1588.2 [M − 2H]2− , 1058.5 [M − 3H]3− ; HR-ESI-MS (positive mode, m/z ) 1589.1852 [M + 2H]2+ (calcd 1589.1850); 1 H-NMR (600 MHz, CD3 OD) δ 5.61 (s, 1H, CH-12), 5.13 (s, 1H, H-1 of Man-4), 4.34–4.33 (m, 2H, H-1 of Gal-6,Gal-6’), 3.00 (m, 2H, β-CH2 of Asn), 2.60 (m, 2H, H-3eq of NeuAc, NeuAc’), 1.42 (s, 3H, CH3 -27), 1.16 (s, 3H, CH3 -29), 1.14 (s, 6H, CH3 -26, 25), 0.99 (s, 3H, CH3 -23), 0.83 (s, 3H, CH3 -24), 0.80 (s, 3H, CH3-28). .. SPR Assay Biacore T200 instruments (GE Healthcare) were used to evaluate the binding affinity of compounds with H1N1 (A/WSN/1933) and H5N1 (A/Hong Kong/483/97), purchased from Sino Biological Inc. (Beijing, China) through SPR, as previously described [ ]. .. In brief, HA protein was immobilized on the surface of a CM5 chip by using the amine coupling approach at a flow rate of 10 μL/min in 10 mM sodium acetate buffer (pH 5.0).

    Injection:

    Article Title: Immobilized sialyloligo-macroligand and its protein binding specificity.
    Article Snippet: We report a chemoenzymatic synthesis of chain-end functionalized sialyllactose-containing glycopolymers with different linkages and their oriented immobilization for glycoarray and SPR-based glyco-biosensor applications. .. We report a chemoenzymatic synthesis of chain-end functionalized sialyllactose-containing glycopolymers with different linkages and their oriented immobilization for glycoarray and SPR-based glyco-biosensor applications. .. We report a chemoenzymatic synthesis of chain-end functionalized sialyllactose-containing glycopolymers with different linkages and their oriented immobilization for glycoarray and SPR-based glyco-biosensor applications.

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    Sino Biological h7n9 haemagglutinin ha monoclonal mouse antibody
    Immunopotentiation following immunization with an H7:ND (1/12, w/w) conjugate. a Immunization scheme in mice. b Measurement of H7 specific IgG amount in mouse sera via ELISA. In total, 50 ng of purified H7 [Influenza antigen A/Anhui/1/2013 <t>(H7N9),</t> NIBSC] per well were coated at the plate. The sera were diluted 1:5000 and a monoclonal mouse anti- H7N9 haemagglutinin/HA antibody (SinoBiological InC.) at concentrations of 0.5, 0.75, 1, 1.25, 2.5, 5, 12.5, 25, 50, 100, 150 μg/mL was applied as a standard, then analysed via ELISA. Specific immune responses were measured at 450 nm after 1 and 2 booster immunizations with H7 (group 1), H7:ND (group 2) and PBS:ND (group 3). The responses were recalculated according the standard values as µg/mL anti H7N9 antibody in the sera. The BSA background was subtracted. A standard curve was built by the help of OD450 values corresponding to known amounts of H7N9 haemagglutinin/HA antibody. The amount of H7 specific IgG antibody in mouse sera was measured via the standard curve. Statistical analyses were performed using the t-test (SigmaPlot) and are presented. A single dot indicates the value of a single mouse serum. SD was included on a single dot that corresponds to an ELISA data variation of a single mouse serum with three replications. The bars indicate the average value of the test groups. P
    H7n9 Haemagglutinin Ha Monoclonal Mouse Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/h7n9 haemagglutinin ha monoclonal mouse antibody/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    h7n9 haemagglutinin ha monoclonal mouse antibody - by Bioz Stars, 2021-06
    94/100 stars
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    95
    Sino Biological recombinant haemagglutinin
    Immunopotentiation following immunization with an H7:ND (1/12, w/w) conjugate. a Immunization scheme in mice. b Measurement of H7 specific IgG amount in mouse sera via ELISA. In total, 50 ng of purified H7 [Influenza antigen A/Anhui/1/2013 <t>(H7N9),</t> NIBSC] per well were coated at the plate. The sera were diluted 1:5000 and a monoclonal mouse anti- H7N9 haemagglutinin/HA antibody (SinoBiological InC.) at concentrations of 0.5, 0.75, 1, 1.25, 2.5, 5, 12.5, 25, 50, 100, 150 μg/mL was applied as a standard, then analysed via ELISA. Specific immune responses were measured at 450 nm after 1 and 2 booster immunizations with H7 (group 1), H7:ND (group 2) and PBS:ND (group 3). The responses were recalculated according the standard values as µg/mL anti H7N9 antibody in the sera. The BSA background was subtracted. A standard curve was built by the help of OD450 values corresponding to known amounts of H7N9 haemagglutinin/HA antibody. The amount of H7 specific IgG antibody in mouse sera was measured via the standard curve. Statistical analyses were performed using the t-test (SigmaPlot) and are presented. A single dot indicates the value of a single mouse serum. SD was included on a single dot that corresponds to an ELISA data variation of a single mouse serum with three replications. The bars indicate the average value of the test groups. P
    Recombinant Haemagglutinin, supplied by Sino Biological, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant haemagglutinin/product/Sino Biological
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant haemagglutinin - by Bioz Stars, 2021-06
    95/100 stars
      Buy from Supplier

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    Immunopotentiation following immunization with an H7:ND (1/12, w/w) conjugate. a Immunization scheme in mice. b Measurement of H7 specific IgG amount in mouse sera via ELISA. In total, 50 ng of purified H7 [Influenza antigen A/Anhui/1/2013 (H7N9), NIBSC] per well were coated at the plate. The sera were diluted 1:5000 and a monoclonal mouse anti- H7N9 haemagglutinin/HA antibody (SinoBiological InC.) at concentrations of 0.5, 0.75, 1, 1.25, 2.5, 5, 12.5, 25, 50, 100, 150 μg/mL was applied as a standard, then analysed via ELISA. Specific immune responses were measured at 450 nm after 1 and 2 booster immunizations with H7 (group 1), H7:ND (group 2) and PBS:ND (group 3). The responses were recalculated according the standard values as µg/mL anti H7N9 antibody in the sera. The BSA background was subtracted. A standard curve was built by the help of OD450 values corresponding to known amounts of H7N9 haemagglutinin/HA antibody. The amount of H7 specific IgG antibody in mouse sera was measured via the standard curve. Statistical analyses were performed using the t-test (SigmaPlot) and are presented. A single dot indicates the value of a single mouse serum. SD was included on a single dot that corresponds to an ELISA data variation of a single mouse serum with three replications. The bars indicate the average value of the test groups. P

    Journal: Journal of Nanobiotechnology

    Article Title: Nanodiamond enhances immune responses in mice against recombinant HA/H7N9 protein

    doi: 10.1186/s12951-017-0305-2

    Figure Lengend Snippet: Immunopotentiation following immunization with an H7:ND (1/12, w/w) conjugate. a Immunization scheme in mice. b Measurement of H7 specific IgG amount in mouse sera via ELISA. In total, 50 ng of purified H7 [Influenza antigen A/Anhui/1/2013 (H7N9), NIBSC] per well were coated at the plate. The sera were diluted 1:5000 and a monoclonal mouse anti- H7N9 haemagglutinin/HA antibody (SinoBiological InC.) at concentrations of 0.5, 0.75, 1, 1.25, 2.5, 5, 12.5, 25, 50, 100, 150 μg/mL was applied as a standard, then analysed via ELISA. Specific immune responses were measured at 450 nm after 1 and 2 booster immunizations with H7 (group 1), H7:ND (group 2) and PBS:ND (group 3). The responses were recalculated according the standard values as µg/mL anti H7N9 antibody in the sera. The BSA background was subtracted. A standard curve was built by the help of OD450 values corresponding to known amounts of H7N9 haemagglutinin/HA antibody. The amount of H7 specific IgG antibody in mouse sera was measured via the standard curve. Statistical analyses were performed using the t-test (SigmaPlot) and are presented. A single dot indicates the value of a single mouse serum. SD was included on a single dot that corresponds to an ELISA data variation of a single mouse serum with three replications. The bars indicate the average value of the test groups. P

    Article Snippet: After blocking with 3% (w/v) bovine serum albumin (BSA), 0.05% (v/v) Tween20 in PBS (PBST) for 2 h, 100 µL of the specific mouse sera dilution [5000 times in 1% (w/v) BSA in PBST] and an anti H7N9 haemagglutinin/HA monoclonal mouse antibody (SinoBiological InC.) at concentrations of 0.5, 0.75, 1, 1.25, 2.5, 5, 12.5, 25, 50, 100, 150 μg/mL was applied on each ELISA plate and incubated at 25 °C for 1 h. Each serum dilution was measured in triplicate.

    Techniques: Mouse Assay, Enzyme-linked Immunosorbent Assay, Purification

    Construct for expression of trimeric Haemagglutinin in plants. H7 was expressed in tobacco leaves under the control of the CaMV 35S promoter. H7 was fused with trimeric motif GCN4-pII to form H7-pII. Recombinant H7 contained His and c-myc tags for affinity chromatography purification and Western blotting, respectively. The LeB4 signal peptide and KDEL motif were used to ensure ER retention. 35S-P: CaMV 35 S promoter; SP: legumin B4 signal peptide; H7: haemagglutinin A/H7N9; GCN4-pII: trimeric motif; His: His-tag; 35S-T: CaMV 35S terminator

    Journal: Journal of Nanobiotechnology

    Article Title: Nanodiamond enhances immune responses in mice against recombinant HA/H7N9 protein

    doi: 10.1186/s12951-017-0305-2

    Figure Lengend Snippet: Construct for expression of trimeric Haemagglutinin in plants. H7 was expressed in tobacco leaves under the control of the CaMV 35S promoter. H7 was fused with trimeric motif GCN4-pII to form H7-pII. Recombinant H7 contained His and c-myc tags for affinity chromatography purification and Western blotting, respectively. The LeB4 signal peptide and KDEL motif were used to ensure ER retention. 35S-P: CaMV 35 S promoter; SP: legumin B4 signal peptide; H7: haemagglutinin A/H7N9; GCN4-pII: trimeric motif; His: His-tag; 35S-T: CaMV 35S terminator

    Article Snippet: After blocking with 3% (w/v) bovine serum albumin (BSA), 0.05% (v/v) Tween20 in PBS (PBST) for 2 h, 100 µL of the specific mouse sera dilution [5000 times in 1% (w/v) BSA in PBST] and an anti H7N9 haemagglutinin/HA monoclonal mouse antibody (SinoBiological InC.) at concentrations of 0.5, 0.75, 1, 1.25, 2.5, 5, 12.5, 25, 50, 100, 150 μg/mL was applied on each ELISA plate and incubated at 25 °C for 1 h. Each serum dilution was measured in triplicate.

    Techniques: Construct, Expressing, Recombinant, Affinity Chromatography, Purification, Western Blot