recombinant ha  (Sino Biological)


Bioz Verified Symbol Sino Biological is a verified supplier
Bioz Manufacturer Symbol Sino Biological manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86
    Name:
    Influenza A H7N9 A Shanghai 1 2013 Hemagglutinin HA Protein His Tag
    Description:
    A DNA sequence encoding the Influenza A virus A Shanghai 1 2013 H7N9 hemagglutinin Met1 Val524 was expressed with a C terminal polyhistidine tag
    Catalog Number:
    40104-V08B
    Price:
    None
    Category:
    recombinant protein
    Host:
    Baculovirus-Insect Cells
    Buy from Supplier


    Structured Review

    Sino Biological recombinant ha
    A DNA sequence encoding the Influenza A virus A Shanghai 1 2013 H7N9 hemagglutinin Met1 Val524 was expressed with a C terminal polyhistidine tag
    https://www.bioz.com/result/recombinant ha/product/Sino Biological
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant ha - by Bioz Stars, 2021-09
    86/100 stars

    Images

    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: A DNA Vaccine That Targets Hemagglutinin to Antigen-Presenting Cells Protects Mice against H7 Influenza
    Article Snippet: .. ELISA plates (Costar 3590) were coated with 0.5 μg/ml recombinant HA [A/Shanghai/1/2013 (H7N9)] (40104-V08B; Sino Biological), blocked with 1% BSA, and incubated with serially diluted serum samples assayed individually (n = 6 to 12/group). ..

    Article Title: Simultaneous Targeting of Multiple Hemagglutinins to APCs for Induction of Broad Immunity against Influenza
    Article Snippet: .. ELISA 96-well plates (Costar 3590) were coated with one of the following recombinant influenza HA proteins: PR8 (11684-V08H; Sino Biological), A/Hong Kong/483/97 (H5N1) (11689-V08H; Sino Biological), A/northern shoveler/California/HKWF115/07 (H6N1) (MBS434125; MyBioSource, San Diego, CA, or 11723-V08H; Sino Biological), A/pintail duck/Alberta/114/1979 (H8N4) (11722-V08H; Sino Biological), A/Hong Kong/1073/99 (H9N2) (11229-V08H; Sino Biological), A/duck/Yangzhou/906/2002 (H11N2) (11705-V08H; Sino Biological), A/black-headed gull/Netherlands/1/00 (H13N8) (11721-V08H; Sino Biological), A/Shanghai/1/2013 (H7N9) (40104-V08B; Sino Biological), or A/Hong Kong/1/1968 (H3N2) (40116-V08B; Sino Biological). ..

    Recombinant:

    Article Title: A DNA Vaccine That Targets Hemagglutinin to Antigen-Presenting Cells Protects Mice against H7 Influenza
    Article Snippet: .. ELISA plates (Costar 3590) were coated with 0.5 μg/ml recombinant HA [A/Shanghai/1/2013 (H7N9)] (40104-V08B; Sino Biological), blocked with 1% BSA, and incubated with serially diluted serum samples assayed individually (n = 6 to 12/group). ..

    Article Title: Simultaneous Targeting of Multiple Hemagglutinins to APCs for Induction of Broad Immunity against Influenza
    Article Snippet: .. ELISA 96-well plates (Costar 3590) were coated with one of the following recombinant influenza HA proteins: PR8 (11684-V08H; Sino Biological), A/Hong Kong/483/97 (H5N1) (11689-V08H; Sino Biological), A/northern shoveler/California/HKWF115/07 (H6N1) (MBS434125; MyBioSource, San Diego, CA, or 11723-V08H; Sino Biological), A/pintail duck/Alberta/114/1979 (H8N4) (11722-V08H; Sino Biological), A/Hong Kong/1073/99 (H9N2) (11229-V08H; Sino Biological), A/duck/Yangzhou/906/2002 (H11N2) (11705-V08H; Sino Biological), A/black-headed gull/Netherlands/1/00 (H13N8) (11721-V08H; Sino Biological), A/Shanghai/1/2013 (H7N9) (40104-V08B; Sino Biological), or A/Hong Kong/1/1968 (H3N2) (40116-V08B; Sino Biological). ..

    Incubation:

    Article Title: A DNA Vaccine That Targets Hemagglutinin to Antigen-Presenting Cells Protects Mice against H7 Influenza
    Article Snippet: .. ELISA plates (Costar 3590) were coated with 0.5 μg/ml recombinant HA [A/Shanghai/1/2013 (H7N9)] (40104-V08B; Sino Biological), blocked with 1% BSA, and incubated with serially diluted serum samples assayed individually (n = 6 to 12/group). ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 80
    Sino Biological recombinant mex4108 ha protein plasma
    Validated miRNA targets are involved in immune response and cell proliferation in the lungs. Expression levels of (A) KRAS , BLIMP1 , and CDK6 (targets of let-7f); (B) SIRT1 , ZAP70 , and MYC (targets of miR-34c); (C) CAMTA1 (target of miR-129); and (D) PTEN (target of miR-18b) in BAL from rhesus macaques (five aged and three young adults) following <t>MEX4108</t> infection are shown. Expression of mRNA was normalized to expression of RPL32 . Changes in gene expression postinfection were determined using one-way repeated-measures ANOVA, followed by Dunnett's multiple comparison post-test. Mean ± SEM are shown. (A–D) *let-7f, miR-34c, miR-129, miR-18-b, KRAS , SIRT1 , CAMTA1 , and PTEN ; ‡ KRAS and ZAP70 ; † CDK6 and MYC . * p
    Recombinant Mex4108 Ha Protein Plasma, supplied by Sino Biological, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant mex4108 ha protein plasma/product/Sino Biological
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant mex4108 ha protein plasma - by Bioz Stars, 2021-09
    80/100 stars
      Buy from Supplier

    94
    Sino Biological recombinant influenza ha proteins
    Increased strain-specific IgG titers after a single MHCII-targeted vaccination. BALB/c mice ( n = 6 per group) were immunized once with 25 μg of plasmids encoding one of the indicated vaccines. ( A ) Sera were harvested at weeks 2, 3, and 4 after vaccination, and IgG responses in sera from each of the vaccine groups were measured by ELISAs against <t>recombinant</t> <t>HA</t> from H5, H6, H8, H9, H11, H13, and H1 (PR8) <t>influenza</t> viruses (mean ± SEM). ** p
    Recombinant Influenza Ha Proteins, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant influenza ha proteins/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant influenza ha proteins - by Bioz Stars, 2021-09
    94/100 stars
      Buy from Supplier

    95
    Sino Biological recombinant ha rha proteins
    Obesity- and age-associated baseline immune history (BIH) profiles to influenza BL-inactivated H3N2 and B viruses and <t>recombinant</t> <t>HA</t> <t>proteins.</t> ( A-H ) Distribution by BMI. The distributions of BMI within the low-BIH group (n=47 for IgG and n=44 for IgA, solid purple line) and the high-BIH group (n=47 for IgG and n=44 for IgA, dashed green line) were plotted for IgG and IgA responses to H3N2 and B viruses and recombinant HA proteins as follows: (A) IgG magnitude against H3N2 viruses; (B) IgG Magnitude to rH3 proteins; (C) IgA magnitude to H3N2 viruses; (D) IgA magnitude to rH3 proteins; (E) IgG breadth to H3N2 viruses; (F) IgG breadth to H3 peptides; (G) IgA breadth to B viruses; and (H) IgA breadth to B proteins. ( I-P ) Distribution by age. The distributions by subjects age group (
    Recombinant Ha Rha Proteins, supplied by Sino Biological, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant ha rha proteins/product/Sino Biological
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant ha rha proteins - by Bioz Stars, 2021-09
    95/100 stars
      Buy from Supplier

    Image Search Results


    Validated miRNA targets are involved in immune response and cell proliferation in the lungs. Expression levels of (A) KRAS , BLIMP1 , and CDK6 (targets of let-7f); (B) SIRT1 , ZAP70 , and MYC (targets of miR-34c); (C) CAMTA1 (target of miR-129); and (D) PTEN (target of miR-18b) in BAL from rhesus macaques (five aged and three young adults) following MEX4108 infection are shown. Expression of mRNA was normalized to expression of RPL32 . Changes in gene expression postinfection were determined using one-way repeated-measures ANOVA, followed by Dunnett's multiple comparison post-test. Mean ± SEM are shown. (A–D) *let-7f, miR-34c, miR-129, miR-18-b, KRAS , SIRT1 , CAMTA1 , and PTEN ; ‡ KRAS and ZAP70 ; † CDK6 and MYC . * p

    Journal: Viral Immunology

    Article Title: microRNAs Regulate Host Immune Response and Pathogenesis During Influenza Infection in Rhesus Macaques

    doi: 10.1089/vim.2015.0074

    Figure Lengend Snippet: Validated miRNA targets are involved in immune response and cell proliferation in the lungs. Expression levels of (A) KRAS , BLIMP1 , and CDK6 (targets of let-7f); (B) SIRT1 , ZAP70 , and MYC (targets of miR-34c); (C) CAMTA1 (target of miR-129); and (D) PTEN (target of miR-18b) in BAL from rhesus macaques (five aged and three young adults) following MEX4108 infection are shown. Expression of mRNA was normalized to expression of RPL32 . Changes in gene expression postinfection were determined using one-way repeated-measures ANOVA, followed by Dunnett's multiple comparison post-test. Mean ± SEM are shown. (A–D) *let-7f, miR-34c, miR-129, miR-18-b, KRAS , SIRT1 , CAMTA1 , and PTEN ; ‡ KRAS and ZAP70 ; † CDK6 and MYC . * p

    Article Snippet: IgG and IgA binding antibody titers were measured in plasma and BAL supernatant by enzyme-linked immunosorbent assay (ELISA) using plates coated with 1 μg/mL recombinant MEX4108 HA protein (plasma) overnight at 4°C (Sino Biological, Inc., Beijing, China).

    Techniques: Expressing, Infection

    Differentially expressed miRNAs in PBMCs following MEX4108. Expression levels of miR-18b (A) , miR-20a (B) , miR-192 (C) , miR-451 (D) , miR-138 (E) , miR-193b (F) , miR-132 (G) , let-7f (H) , and miR-146b (I) in PBMCs from rhesus macaques ( n = 11; five aged and six young adults) following MEX4108 infection were determined using qRT-PCR. Expression of miRNAs was normalized to expression of U6 snRNA. Changes in microRNA expression postinfection were determined using one-way repeated-measures ANOVA, followed by Dunnett's multiple comparison post-test. Mean ± SEM are shown. * p

    Journal: Viral Immunology

    Article Title: microRNAs Regulate Host Immune Response and Pathogenesis During Influenza Infection in Rhesus Macaques

    doi: 10.1089/vim.2015.0074

    Figure Lengend Snippet: Differentially expressed miRNAs in PBMCs following MEX4108. Expression levels of miR-18b (A) , miR-20a (B) , miR-192 (C) , miR-451 (D) , miR-138 (E) , miR-193b (F) , miR-132 (G) , let-7f (H) , and miR-146b (I) in PBMCs from rhesus macaques ( n = 11; five aged and six young adults) following MEX4108 infection were determined using qRT-PCR. Expression of miRNAs was normalized to expression of U6 snRNA. Changes in microRNA expression postinfection were determined using one-way repeated-measures ANOVA, followed by Dunnett's multiple comparison post-test. Mean ± SEM are shown. * p

    Article Snippet: IgG and IgA binding antibody titers were measured in plasma and BAL supernatant by enzyme-linked immunosorbent assay (ELISA) using plates coated with 1 μg/mL recombinant MEX4108 HA protein (plasma) overnight at 4°C (Sino Biological, Inc., Beijing, China).

    Techniques: Expressing, Infection, Quantitative RT-PCR

    MEX4108 infection results in increased frequency of pDCs and robust production of cytokine, chemokine, and growth factor levels in the lungs. (A) The frequencies of DCs (lin − CD14 − HLA-DR + ) and macrophages/monocytes (lin − CD14 + HLA-DR − ) in BAL cells were measured by flow cytometry. (B) The frequencies of mDC (CD123 − CD11c + ) and pDC (CD123 + CD11c − ) in BAL cells were measured by flow cytometry. Longitudinal analyses of the frequency of immune cells within BAL were carried out using a one-way repeated-measures ANOVA model, followed by Dunnett's multiple comparison post-test to explore differences between days postinfection and baseline (day 0) values. Mean ± SEM are shown. * p

    Journal: Viral Immunology

    Article Title: microRNAs Regulate Host Immune Response and Pathogenesis During Influenza Infection in Rhesus Macaques

    doi: 10.1089/vim.2015.0074

    Figure Lengend Snippet: MEX4108 infection results in increased frequency of pDCs and robust production of cytokine, chemokine, and growth factor levels in the lungs. (A) The frequencies of DCs (lin − CD14 − HLA-DR + ) and macrophages/monocytes (lin − CD14 + HLA-DR − ) in BAL cells were measured by flow cytometry. (B) The frequencies of mDC (CD123 − CD11c + ) and pDC (CD123 + CD11c − ) in BAL cells were measured by flow cytometry. Longitudinal analyses of the frequency of immune cells within BAL were carried out using a one-way repeated-measures ANOVA model, followed by Dunnett's multiple comparison post-test to explore differences between days postinfection and baseline (day 0) values. Mean ± SEM are shown. * p

    Article Snippet: IgG and IgA binding antibody titers were measured in plasma and BAL supernatant by enzyme-linked immunosorbent assay (ELISA) using plates coated with 1 μg/mL recombinant MEX4108 HA protein (plasma) overnight at 4°C (Sino Biological, Inc., Beijing, China).

    Techniques: Infection, Flow Cytometry

    Model of differentially expressed microRNAs, gene targets, and immune response in the lungs following MEX4108 infection.

    Journal: Viral Immunology

    Article Title: microRNAs Regulate Host Immune Response and Pathogenesis During Influenza Infection in Rhesus Macaques

    doi: 10.1089/vim.2015.0074

    Figure Lengend Snippet: Model of differentially expressed microRNAs, gene targets, and immune response in the lungs following MEX4108 infection.

    Article Snippet: IgG and IgA binding antibody titers were measured in plasma and BAL supernatant by enzyme-linked immunosorbent assay (ELISA) using plates coated with 1 μg/mL recombinant MEX4108 HA protein (plasma) overnight at 4°C (Sino Biological, Inc., Beijing, China).

    Techniques: Infection

    Pandemic H1N1 virus replicates to similar levels in young and aged macaques. Viral loads were measured using qRT-PCR using primers and probes specific for MEX4108 hemagglutinin (HA) in throat swabs (A) , nasal swabs (B) , ocular swabs (C) , and BAL fluid (D) . Viral genome copy number data were log transformed with base 10 and longitudinal changes of viral genome copy number between aged and young adults were compared using a two-way ANOVA, followed by Bonferroni's multiple comparison post-test to determine differences in viral load. Longitudinal changes were compared using repeated-measures ANOVA, followed by Dunnett's multiple comparison post-test to explore differences between days postinfection and baseline (day 0) values, mean ± SEM are shown. (A–D) * for aged animals; ‡ for young adult animals; *** ,‡‡ p

    Journal: Viral Immunology

    Article Title: microRNAs Regulate Host Immune Response and Pathogenesis During Influenza Infection in Rhesus Macaques

    doi: 10.1089/vim.2015.0074

    Figure Lengend Snippet: Pandemic H1N1 virus replicates to similar levels in young and aged macaques. Viral loads were measured using qRT-PCR using primers and probes specific for MEX4108 hemagglutinin (HA) in throat swabs (A) , nasal swabs (B) , ocular swabs (C) , and BAL fluid (D) . Viral genome copy number data were log transformed with base 10 and longitudinal changes of viral genome copy number between aged and young adults were compared using a two-way ANOVA, followed by Bonferroni's multiple comparison post-test to determine differences in viral load. Longitudinal changes were compared using repeated-measures ANOVA, followed by Dunnett's multiple comparison post-test to explore differences between days postinfection and baseline (day 0) values, mean ± SEM are shown. (A–D) * for aged animals; ‡ for young adult animals; *** ,‡‡ p

    Article Snippet: IgG and IgA binding antibody titers were measured in plasma and BAL supernatant by enzyme-linked immunosorbent assay (ELISA) using plates coated with 1 μg/mL recombinant MEX4108 HA protein (plasma) overnight at 4°C (Sino Biological, Inc., Beijing, China).

    Techniques: Quantitative RT-PCR, Transformation Assay

    Differentially expressed miRNAs in BAL cells following MEX4108. Expression levels of let-7f (A) , miR-34c (B) , miR-129 (C) , miR-18b (D) , miR-146b (E) , miR-132 (F) , miR-192 (G) , and miR-138 (H) in BAL cells from rhesus macaques ( n = 8; five aged and three young adults) following MEX4108 infection were determined using qRT-PCR. Expression of miRNAs was normalized to expression of U6 snRNA. Changes in microRNA expression postinfection were determined using one-way repeated-measures ANOVA, followed by Dunnett's multiple comparison post-test. Mean ± SEM are shown. * p

    Journal: Viral Immunology

    Article Title: microRNAs Regulate Host Immune Response and Pathogenesis During Influenza Infection in Rhesus Macaques

    doi: 10.1089/vim.2015.0074

    Figure Lengend Snippet: Differentially expressed miRNAs in BAL cells following MEX4108. Expression levels of let-7f (A) , miR-34c (B) , miR-129 (C) , miR-18b (D) , miR-146b (E) , miR-132 (F) , miR-192 (G) , and miR-138 (H) in BAL cells from rhesus macaques ( n = 8; five aged and three young adults) following MEX4108 infection were determined using qRT-PCR. Expression of miRNAs was normalized to expression of U6 snRNA. Changes in microRNA expression postinfection were determined using one-way repeated-measures ANOVA, followed by Dunnett's multiple comparison post-test. Mean ± SEM are shown. * p

    Article Snippet: IgG and IgA binding antibody titers were measured in plasma and BAL supernatant by enzyme-linked immunosorbent assay (ELISA) using plates coated with 1 μg/mL recombinant MEX4108 HA protein (plasma) overnight at 4°C (Sino Biological, Inc., Beijing, China).

    Techniques: Expressing, Infection, Quantitative RT-PCR

    Increased strain-specific IgG titers after a single MHCII-targeted vaccination. BALB/c mice ( n = 6 per group) were immunized once with 25 μg of plasmids encoding one of the indicated vaccines. ( A ) Sera were harvested at weeks 2, 3, and 4 after vaccination, and IgG responses in sera from each of the vaccine groups were measured by ELISAs against recombinant HA from H5, H6, H8, H9, H11, H13, and H1 (PR8) influenza viruses (mean ± SEM). ** p

    Journal: The Journal of Immunology Author Choice

    Article Title: Simultaneous Targeting of Multiple Hemagglutinins to APCs for Induction of Broad Immunity against Influenza

    doi: 10.4049/jimmunol.1701088

    Figure Lengend Snippet: Increased strain-specific IgG titers after a single MHCII-targeted vaccination. BALB/c mice ( n = 6 per group) were immunized once with 25 μg of plasmids encoding one of the indicated vaccines. ( A ) Sera were harvested at weeks 2, 3, and 4 after vaccination, and IgG responses in sera from each of the vaccine groups were measured by ELISAs against recombinant HA from H5, H6, H8, H9, H11, H13, and H1 (PR8) influenza viruses (mean ± SEM). ** p

    Article Snippet: ELISA 96-well plates (Costar 3590) were coated with one of the following recombinant influenza HA proteins: PR8 (11684-V08H; Sino Biological), A/Hong Kong/483/97 (H5N1) (11689-V08H; Sino Biological), A/northern shoveler/California/HKWF115/07 (H6N1) (MBS434125; MyBioSource, San Diego, CA, or 11723-V08H; Sino Biological), A/pintail duck/Alberta/114/1979 (H8N4) (11722-V08H; Sino Biological), A/Hong Kong/1073/99 (H9N2) (11229-V08H; Sino Biological), A/duck/Yangzhou/906/2002 (H11N2) (11705-V08H; Sino Biological), A/black-headed gull/Netherlands/1/00 (H13N8) (11721-V08H; Sino Biological), A/Shanghai/1/2013 (H7N9) (40104-V08B; Sino Biological), or A/Hong Kong/1/1968 (H3N2) (40116-V08B; Sino Biological).

    Techniques: Mouse Assay, Recombinant

    Ab responses against all included HAs after DNA immunization with vaccine mixtures. BALB/c mice ( n = 6 per group) were immunized three times (weeks 0, 4, and 12) with 25 μg of DNA of the indicated vaccine (arrowheads). Serum samples were harvested 2–3 wk after each vaccine delivery, and HA-specific IgG responses were measured in sandwich ELISAs against recombinant HA from H5, H6, H8, H9, H11, and H13 influenza viruses (mean ± SEM). * p

    Journal: The Journal of Immunology Author Choice

    Article Title: Simultaneous Targeting of Multiple Hemagglutinins to APCs for Induction of Broad Immunity against Influenza

    doi: 10.4049/jimmunol.1701088

    Figure Lengend Snippet: Ab responses against all included HAs after DNA immunization with vaccine mixtures. BALB/c mice ( n = 6 per group) were immunized three times (weeks 0, 4, and 12) with 25 μg of DNA of the indicated vaccine (arrowheads). Serum samples were harvested 2–3 wk after each vaccine delivery, and HA-specific IgG responses were measured in sandwich ELISAs against recombinant HA from H5, H6, H8, H9, H11, and H13 influenza viruses (mean ± SEM). * p

    Article Snippet: ELISA 96-well plates (Costar 3590) were coated with one of the following recombinant influenza HA proteins: PR8 (11684-V08H; Sino Biological), A/Hong Kong/483/97 (H5N1) (11689-V08H; Sino Biological), A/northern shoveler/California/HKWF115/07 (H6N1) (MBS434125; MyBioSource, San Diego, CA, or 11723-V08H; Sino Biological), A/pintail duck/Alberta/114/1979 (H8N4) (11722-V08H; Sino Biological), A/Hong Kong/1073/99 (H9N2) (11229-V08H; Sino Biological), A/duck/Yangzhou/906/2002 (H11N2) (11705-V08H; Sino Biological), A/black-headed gull/Netherlands/1/00 (H13N8) (11721-V08H; Sino Biological), A/Shanghai/1/2013 (H7N9) (40104-V08B; Sino Biological), or A/Hong Kong/1/1968 (H3N2) (40116-V08B; Sino Biological).

    Techniques: Mouse Assay, Recombinant

    Obesity- and age-associated baseline immune history (BIH) profiles to influenza BL-inactivated H3N2 and B viruses and recombinant HA proteins. ( A-H ) Distribution by BMI. The distributions of BMI within the low-BIH group (n=47 for IgG and n=44 for IgA, solid purple line) and the high-BIH group (n=47 for IgG and n=44 for IgA, dashed green line) were plotted for IgG and IgA responses to H3N2 and B viruses and recombinant HA proteins as follows: (A) IgG magnitude against H3N2 viruses; (B) IgG Magnitude to rH3 proteins; (C) IgA magnitude to H3N2 viruses; (D) IgA magnitude to rH3 proteins; (E) IgG breadth to H3N2 viruses; (F) IgG breadth to H3 peptides; (G) IgA breadth to B viruses; and (H) IgA breadth to B proteins. ( I-P ) Distribution by age. The distributions by subjects age group (

    Journal: medRxiv

    Article Title: Obesity is associated with an altered baseline and post-vaccination influenza antibody repertoire

    doi: 10.1101/2021.03.02.21252785

    Figure Lengend Snippet: Obesity- and age-associated baseline immune history (BIH) profiles to influenza BL-inactivated H3N2 and B viruses and recombinant HA proteins. ( A-H ) Distribution by BMI. The distributions of BMI within the low-BIH group (n=47 for IgG and n=44 for IgA, solid purple line) and the high-BIH group (n=47 for IgG and n=44 for IgA, dashed green line) were plotted for IgG and IgA responses to H3N2 and B viruses and recombinant HA proteins as follows: (A) IgG magnitude against H3N2 viruses; (B) IgG Magnitude to rH3 proteins; (C) IgA magnitude to H3N2 viruses; (D) IgA magnitude to rH3 proteins; (E) IgG breadth to H3N2 viruses; (F) IgG breadth to H3 peptides; (G) IgA breadth to B viruses; and (H) IgA breadth to B proteins. ( I-P ) Distribution by age. The distributions by subjects age group (

    Article Snippet: Recombinant HA (rHA) proteins were purchased from Sino biological (China) as purified His-tagged proteins.

    Techniques: Recombinant

    Baseline Immune History (BIH) and post vaccination IgA responses in healthy weight (HW) and obese (OB) individuals to a panel of historical influenza recombinant proteins (rHA). Baseline and post-vaccination serum samples from 82 healthy-weight (HW) and 92 obese (OB) subjects were hybridized with an antigen microarray spotted with 34 BPL-inactivated influenza viruses and 23 recombinant HA (rHA) proteins that included the three vaccine strains used in the study, for profiling of IgA binding. (A-C) IgA binding to the recombinant HA of H1N1 A/California/7/2009, H3N2 A/Perth/16/2009 and B/Brisbane/60/2008 vaccine strains. ( D-F ) The magnitude of IgA antibodies bound to a panel of 8 recombinant H1 (rH1) proteins (D); a panel of 10 recombinant H3 (rH3) proteins; (E) and a panel of recombinant HA antigens of 5 B strains (F). (G-I) The breadth of IgA antibodies bound to a panel of recombinant HA proteins, including: 8 rH1 proteins (G); 10 rH3 proteins (H); and 5 recombinant HA proteins of 5 B strains (l) proteins. The four box plots in the left portion of each panel summarize the baseline (L- > R: HW: light green, Ob: light blue) and the 30-day post-vaccination (L- > R: HW: dark green, Ob: dark blue) binding responses. The two boxplots on the right side of each panel represent the fold increase (L- > R: HW: green, Ob: blue). Lines represent the median fluorescence intensity (MFI), the boxes denote the 25th and 75th percentiles, and the error bars represent 1.5 times the interquartile range. Statistical significance was assessed using the Wilcoxon signed rank test (pre vs. post) and the Wilcoxon rank-sum test (HW vs. Ob). * p

    Journal: medRxiv

    Article Title: Obesity is associated with an altered baseline and post-vaccination influenza antibody repertoire

    doi: 10.1101/2021.03.02.21252785

    Figure Lengend Snippet: Baseline Immune History (BIH) and post vaccination IgA responses in healthy weight (HW) and obese (OB) individuals to a panel of historical influenza recombinant proteins (rHA). Baseline and post-vaccination serum samples from 82 healthy-weight (HW) and 92 obese (OB) subjects were hybridized with an antigen microarray spotted with 34 BPL-inactivated influenza viruses and 23 recombinant HA (rHA) proteins that included the three vaccine strains used in the study, for profiling of IgA binding. (A-C) IgA binding to the recombinant HA of H1N1 A/California/7/2009, H3N2 A/Perth/16/2009 and B/Brisbane/60/2008 vaccine strains. ( D-F ) The magnitude of IgA antibodies bound to a panel of 8 recombinant H1 (rH1) proteins (D); a panel of 10 recombinant H3 (rH3) proteins; (E) and a panel of recombinant HA antigens of 5 B strains (F). (G-I) The breadth of IgA antibodies bound to a panel of recombinant HA proteins, including: 8 rH1 proteins (G); 10 rH3 proteins (H); and 5 recombinant HA proteins of 5 B strains (l) proteins. The four box plots in the left portion of each panel summarize the baseline (L- > R: HW: light green, Ob: light blue) and the 30-day post-vaccination (L- > R: HW: dark green, Ob: dark blue) binding responses. The two boxplots on the right side of each panel represent the fold increase (L- > R: HW: green, Ob: blue). Lines represent the median fluorescence intensity (MFI), the boxes denote the 25th and 75th percentiles, and the error bars represent 1.5 times the interquartile range. Statistical significance was assessed using the Wilcoxon signed rank test (pre vs. post) and the Wilcoxon rank-sum test (HW vs. Ob). * p

    Article Snippet: Recombinant HA (rHA) proteins were purchased from Sino biological (China) as purified His-tagged proteins.

    Techniques: Recombinant, Microarray, Binding Assay, Fluorescence

    Heterogeneity of baseline (BIH) and 30-day post-vaccination influenza immune history profiles to recombinant HA (rHA) proteins. Panels (A-B): The entire cohort of 205 subjects, 89 healthy-weight (HW) and 100 obese (OB) subjects were assigned to quartiles (low-BIH: n=47, mid-BIH: n=95 and high-BIH: n=47) based on the magnitudes of (A) IgG responses and (B) IgA responses (low-BIH: n=44, mid-BIH: n=86 and high-BIH: n=44) to recombinant HA proteins of the H1N1 subtype strains. ( C-F ) Spider plots of the BIH and post-vaccination binding responses of individual subjects where each vertex represents the normalized binding of IgG or IgA to a single influenza strain rHA. Baseline and post-vaccination antibody repertoires are denoted by blue and orange spider plots, respectively (light and dark grey in Black and white). The ID, BMI and age of each subject is presented above each spider graph. The numbers on the inside of the outer circles denote the year each strain was isolated, and the three vaccine strains are denoted by the colored dots near the inner circles. Counter-clockwise rHA from: H1N1 (purple segment of circle) strains 33-09-33; H3N2 (red segment of circle) strains 89-13; and B (light blue segment of circle) strains 13-88. Isolates appear in the same order as listed in Table S1 . Representative spider graphs are presented for HW subjects (C and D) and OB subjects (E and F) for subjects whose responses were in the low IgG BIH group (C and E) or the high IgG BIH group (D and F).

    Journal: medRxiv

    Article Title: Obesity is associated with an altered baseline and post-vaccination influenza antibody repertoire

    doi: 10.1101/2021.03.02.21252785

    Figure Lengend Snippet: Heterogeneity of baseline (BIH) and 30-day post-vaccination influenza immune history profiles to recombinant HA (rHA) proteins. Panels (A-B): The entire cohort of 205 subjects, 89 healthy-weight (HW) and 100 obese (OB) subjects were assigned to quartiles (low-BIH: n=47, mid-BIH: n=95 and high-BIH: n=47) based on the magnitudes of (A) IgG responses and (B) IgA responses (low-BIH: n=44, mid-BIH: n=86 and high-BIH: n=44) to recombinant HA proteins of the H1N1 subtype strains. ( C-F ) Spider plots of the BIH and post-vaccination binding responses of individual subjects where each vertex represents the normalized binding of IgG or IgA to a single influenza strain rHA. Baseline and post-vaccination antibody repertoires are denoted by blue and orange spider plots, respectively (light and dark grey in Black and white). The ID, BMI and age of each subject is presented above each spider graph. The numbers on the inside of the outer circles denote the year each strain was isolated, and the three vaccine strains are denoted by the colored dots near the inner circles. Counter-clockwise rHA from: H1N1 (purple segment of circle) strains 33-09-33; H3N2 (red segment of circle) strains 89-13; and B (light blue segment of circle) strains 13-88. Isolates appear in the same order as listed in Table S1 . Representative spider graphs are presented for HW subjects (C and D) and OB subjects (E and F) for subjects whose responses were in the low IgG BIH group (C and E) or the high IgG BIH group (D and F).

    Article Snippet: Recombinant HA (rHA) proteins were purchased from Sino biological (China) as purified His-tagged proteins.

    Techniques: Recombinant, Binding Assay, Isolation

    Baseline Immune History (BIH) and 30-day post vaccination IgG responses in healthy weight (HW) and obese (OB) individuals to a panel of historical influenza recombinant proteins (rHA). Baseline and post-vaccination serum samples from 89 healthy-weight (HW) and 100 obese (OB) subjects were hybridized with an antigen microarray spotted with 34 BPL-inactivated influenza viruses and 23 recombinant HA (rHA) proteins that included the three vaccine strains used in the study, for profiling of IgG binding (see Table S1 for IgA binding). (A-C) IgG binding to the recombinant HA of H1N1 A/California/7/2009, H3N2 A/Perth/16/2009 and B/Brisbane/60/2008 vaccine strains. ( D-F ) The magnitude of IgG antibodies bound to a panel of 8 recombinant H1 (rH1) proteins (D); a panel of 10 recombinant H3 (rH3) proteins; (E) and a panel of recombinant HA antigens of 5 B strains (F). ( G-I) The breadth of IgG antibodies bound to a panel of recombinant HA proteins, including: 8 rH1 proteins (G); 10 rH3 proteins (H); and 5 recombinant HA proteins of 5 B strains (l) proteins. The four box plots in the left portion of each panel summarize the baseline (L- > R: HW: light green, OB: light blue) and the 30-day post-vaccination (L- > R: HW: dark green, OB: dark blue) binding responses. The two boxplots on the right side of each panel represent the fold increase (L- > R: HW: green, OB: blue). Lines represent the median fluorescence intensity (MFI), the boxes denote the 25th and 75th percentiles, and the error bars represent 1.5 times the interquartile range. Statistical significance was assessed using the Wilcoxon signed rank test (pre vs. post) and the Wilcoxon rank-sum test (HW vs. OB). * p

    Journal: medRxiv

    Article Title: Obesity is associated with an altered baseline and post-vaccination influenza antibody repertoire

    doi: 10.1101/2021.03.02.21252785

    Figure Lengend Snippet: Baseline Immune History (BIH) and 30-day post vaccination IgG responses in healthy weight (HW) and obese (OB) individuals to a panel of historical influenza recombinant proteins (rHA). Baseline and post-vaccination serum samples from 89 healthy-weight (HW) and 100 obese (OB) subjects were hybridized with an antigen microarray spotted with 34 BPL-inactivated influenza viruses and 23 recombinant HA (rHA) proteins that included the three vaccine strains used in the study, for profiling of IgG binding (see Table S1 for IgA binding). (A-C) IgG binding to the recombinant HA of H1N1 A/California/7/2009, H3N2 A/Perth/16/2009 and B/Brisbane/60/2008 vaccine strains. ( D-F ) The magnitude of IgG antibodies bound to a panel of 8 recombinant H1 (rH1) proteins (D); a panel of 10 recombinant H3 (rH3) proteins; (E) and a panel of recombinant HA antigens of 5 B strains (F). ( G-I) The breadth of IgG antibodies bound to a panel of recombinant HA proteins, including: 8 rH1 proteins (G); 10 rH3 proteins (H); and 5 recombinant HA proteins of 5 B strains (l) proteins. The four box plots in the left portion of each panel summarize the baseline (L- > R: HW: light green, OB: light blue) and the 30-day post-vaccination (L- > R: HW: dark green, OB: dark blue) binding responses. The two boxplots on the right side of each panel represent the fold increase (L- > R: HW: green, OB: blue). Lines represent the median fluorescence intensity (MFI), the boxes denote the 25th and 75th percentiles, and the error bars represent 1.5 times the interquartile range. Statistical significance was assessed using the Wilcoxon signed rank test (pre vs. post) and the Wilcoxon rank-sum test (HW vs. OB). * p

    Article Snippet: Recombinant HA (rHA) proteins were purchased from Sino biological (China) as purified His-tagged proteins.

    Techniques: Recombinant, Microarray, Binding Assay, Fluorescence