recombinant influenza a virus h1n1 hemagglutinin  (Sino Biological)


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    Name:
    Influenza A H1N1 Hemagglutinin HA Protein
    Description:
    A DNA sequence encoding the extracellular domain of Influenza A virus A Brisbane 59 2007 H1N1 ACA28844 1 hemagglutinin Met 1 Gln 528 Native HA1 HA2 uncleaved was expressed with a C terminal polyhistidine tag
    Catalog Number:
    11052-V08H
    Price:
    None
    Category:
    recombinant protein
    Host:
    HEK293 Cells
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    Structured Review

    Sino Biological recombinant influenza a virus h1n1 hemagglutinin
    A DNA sequence encoding the extracellular domain of Influenza A virus A Brisbane 59 2007 H1N1 ACA28844 1 hemagglutinin Met 1 Gln 528 Native HA1 HA2 uncleaved was expressed with a C terminal polyhistidine tag
    https://www.bioz.com/result/recombinant influenza a virus h1n1 hemagglutinin/product/Sino Biological
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant influenza a virus h1n1 hemagglutinin - by Bioz Stars, 2021-07
    96/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Seasonal H1N1 Influenza Virus Infection Induces Cross-Protective Pandemic H1N1 Virus Immunity through a CD8-Independent, B Cell-Dependent Mechanism
    Article Snippet: Serum and lung homogenate samples were assessed for antibody level by ELISA. .. Briefly, ELISA plates were coated with inactivated split pandemic H1N1 A/California/07/2009 virus (5 μg of HA/ml), recombinant H1N1 virus (seasonal H1N1 A/Bribane/59/2007 virus, pandemic H1N1 virus A/California/07/2009; Sino Biological, Inc., China) HA (5 μg/ml), or recombinant H1N1 virus (A/Puerto Rico/8/34) NP (5 μg/ml) overnight at 4°C. ..

    Article Title: Preexisting Antibody-Dependent Cellular Cytotoxicity–Activating Antibody Responses Are Stable Longitudinally and Cross-reactive Responses Are Not Boosted by Recent Influenza Exposure
    Article Snippet: .. Briefly, U-bottomed enzyme-linked immunosorbent assay (ELISA) plates are coated overnight with recombinant influenza virus proteins, H7 HA (H7N9; A/Anhui/01/2013), H1 HA (H1N1; A/California/04/2009), and NP (H7N9; A/Anhui/01/2013; > 95% homology with H1N1-derived NP; 400 ng/well; SinoBiological) in phosphate-buffered saline (PBS). .. Background ADCC activity by NK cells was determined by analysis of paired serum specimens with plate-bound nonspecific protein (allantoic fluid), with positive controls including purified CD16 antibody (Biolegend) for coating and pooled human sera (n = 4 donors pooled) tested against each protein.

    Recombinant:

    Article Title: Seasonal H1N1 Influenza Virus Infection Induces Cross-Protective Pandemic H1N1 Virus Immunity through a CD8-Independent, B Cell-Dependent Mechanism
    Article Snippet: Serum and lung homogenate samples were assessed for antibody level by ELISA. .. Briefly, ELISA plates were coated with inactivated split pandemic H1N1 A/California/07/2009 virus (5 μg of HA/ml), recombinant H1N1 virus (seasonal H1N1 A/Bribane/59/2007 virus, pandemic H1N1 virus A/California/07/2009; Sino Biological, Inc., China) HA (5 μg/ml), or recombinant H1N1 virus (A/Puerto Rico/8/34) NP (5 μg/ml) overnight at 4°C. ..

    Article Title: Rapid, Sensitive, and Selective Detection of H5 Hemagglutinin from Avian Influenza Virus Using an Immunowall Device
    Article Snippet: Phosphate-buffered saline (PBS; pH 7.4) solution and 9H -(1,3-dichloro-9,9-dimethylacridin-2-one-7-yl) phosphate (DDAO phosphate) diammonium salt were purchased from Thermo Fisher Scientific, Inc. (Waltham, MA). .. Mouse anti-H5N1 HA monoclonal antibody, recombinant H5N1 HA, recombinant H1N1 HA, recombinant H3N2 HA, recombinant H7N9 HA, and rabbit anti-avian influenza A HA polyclonal antibody were purchased from Sino Biological, Inc. (Beijing, China). .. DyLight 650-labeled goat antirabbit polyclonal antibody and alkaline phosphatase-labeled goat antirabbit polyclonal antibody were purchased from Abcam (Cambridge, MA).

    Article Title: Preexisting Antibody-Dependent Cellular Cytotoxicity–Activating Antibody Responses Are Stable Longitudinally and Cross-reactive Responses Are Not Boosted by Recent Influenza Exposure
    Article Snippet: .. Briefly, U-bottomed enzyme-linked immunosorbent assay (ELISA) plates are coated overnight with recombinant influenza virus proteins, H7 HA (H7N9; A/Anhui/01/2013), H1 HA (H1N1; A/California/04/2009), and NP (H7N9; A/Anhui/01/2013; > 95% homology with H1N1-derived NP; 400 ng/well; SinoBiological) in phosphate-buffered saline (PBS). .. Background ADCC activity by NK cells was determined by analysis of paired serum specimens with plate-bound nonspecific protein (allantoic fluid), with positive controls including purified CD16 antibody (Biolegend) for coating and pooled human sera (n = 4 donors pooled) tested against each protein.

    Article Title: Single-Step Detection of the Influenza Virus Hemagglutinin Using Bacterially-Produced Quenchbodies
    Article Snippet: Anti DYKDDDDK-tag antibody beads and the DYKDDDDK peptide were obtained from Wako Pure Chemicals (Osaka, Japan). .. The recombinant HA protein from A/California/04/2009 H1N1 was obtained from Sino Biological (Beijing, China). ..

    Article Title: Inhibition of influenza A virus infection by ginsenosides
    Article Snippet: For the determination of viral loads, lung tissues were collected on days 3 and 6 post infection (p.i.) from those animals who did not reach to the humane endpoint of 20% loss in body weight [ ]. .. Immunoblot assayDifferent concentrations of ginsenosides Rb1 were mixed with recombinant HA protein -Influenza A virus H1N1 (A/California/07/2009) (Sino Biological Inc., Beijing, China) and incubated at 37°C for 1 hour and then was spotted onto PVDF membrane. .. Immunoblotting was performed with Influenza A virus HA antibody (Santa cruz) and secondary goat anti-mouse IgG peroxidase conjugate (Calbiochem) and visualized by enhanced chemiluminescence (ECL).

    Conjugation Assay:

    Article Title: Development and evaluation of a paramagnetic nanoparticle based immunochromatographic strip for specific detection of 2009 H1N1 influenza virus.
    Article Snippet: .. Influenza A/H1N1 virus spreads worldwide and has been a threat to human health and the poultry industry. .. Influenza A/H1N1 virus spreads worldwide and has been a threat to human health and the poultry industry.

    Modification:

    Article Title: Development and evaluation of a paramagnetic nanoparticle based immunochromatographic strip for specific detection of 2009 H1N1 influenza virus.
    Article Snippet: .. Influenza A/H1N1 virus spreads worldwide and has been a threat to human health and the poultry industry. .. Influenza A/H1N1 virus spreads worldwide and has been a threat to human health and the poultry industry.

    Incubation:

    Article Title: Inhibition of influenza A virus infection by ginsenosides
    Article Snippet: For the determination of viral loads, lung tissues were collected on days 3 and 6 post infection (p.i.) from those animals who did not reach to the humane endpoint of 20% loss in body weight [ ]. .. Immunoblot assayDifferent concentrations of ginsenosides Rb1 were mixed with recombinant HA protein -Influenza A virus H1N1 (A/California/07/2009) (Sino Biological Inc., Beijing, China) and incubated at 37°C for 1 hour and then was spotted onto PVDF membrane. .. Immunoblotting was performed with Influenza A virus HA antibody (Santa cruz) and secondary goat anti-mouse IgG peroxidase conjugate (Calbiochem) and visualized by enhanced chemiluminescence (ECL).

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  • 94
    Sino Biological influenza a h1n1 hemagglutinin ha antibody mouse mab
    Overall experimental study design for T-cell epitope discovery in Pandemrix-associated NT1. As a first step, <t>influenza</t> A (H1N1) virus HA, NA, and NP peptide T cell recognition was tested with spleen cells from Pandemrix-vaccinated HLA-DQ6.2 mice, restimulated with pools of 5 peptides each (15-mers). Pools that stimulated IFN -γ or IL-2 expression were broken up, and single peptides tested either with spleen cells from additional Pandemrix-vaccinated HLA-DQ6.2 mice, or PBMC from Pandemrix-vaccinated HLA-DQB1*0602 positive individuals. As a second step, recognition of single peptides was then tested with PBMC from pediatric Pandemrix-associated NT1 patients, and healthy Pandemrix-vaccinated controls. As a third step, influenza A (H1N1) T cell peptides that showed increased stimulation of IFN - γ or IL-2 secretion in patients vs. controls were validated and mapped. Cross-reactive T cell self-epitopes were predicted by BLAST against human proteome, and recognition tested with PBMC from pediatric Pandemrix-associated NT1 patients, and healthy Pandemrix-vaccinated controls.
    Influenza A H1n1 Hemagglutinin Ha Antibody Mouse Mab, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/influenza a h1n1 hemagglutinin ha antibody mouse mab/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    influenza a h1n1 hemagglutinin ha antibody mouse mab - by Bioz Stars, 2021-07
    94/100 stars
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    94
    Sino Biological influenza a virus hemagglutinin ha antibody rabbit mab
    Effect of λ-CGN on <t>influenza</t> A infection in vivo . BALB/c mice were mock-infected (black) or intranasally infected with maPR8 at 5 MLD50 (red). As test groups, the virus was preincubated at room temperature for 30 min with λ-CGN at a lower dose (1 mg/kg/d, bright green) or a higher dose (5 mg/kg/d, dark green), followed by intranasal administration. Control mice received oral OSV-P twice a day (10 mg/kg/d) at 8-h intervals, starting at 4 h before viral infection (blue). Body weight (A) and mortality (B) of mice were measured every day from Days 0 to 14 post-infection. Data are expressed as the mean ± SEM from five mice
    Influenza A Virus Hemagglutinin Ha Antibody Rabbit Mab, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/influenza a virus hemagglutinin ha antibody rabbit mab/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    influenza a virus hemagglutinin ha antibody rabbit mab - by Bioz Stars, 2021-07
    94/100 stars
      Buy from Supplier

    96
    Sino Biological recombinant ha protein influenza a virus h1n1
    Antiviral activity of ginseng extract (GE) against 2009 pandemic <t>H1N1</t> <t>influenza</t> A virus infection. (A) A dose dependent reduction in viral titer was observed when MDCK cells were infected with GE-pretreated A/Nanchang/8002/2009 (H1N1) virus. Protective effect of GE was observed on (B) weight loss and (C) survival of mice infected with A/Nanchang/8002/2009 (H1N1) virus. GE oral_ mice treated with 80mg/kg of GE by oral route. GE_ mice were infected with a mixture of 10 3 EID 50 of virus and GE. Mock_ there was no GE treatment given. *** P
    Recombinant Ha Protein Influenza A Virus H1n1, supplied by Sino Biological, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant ha protein influenza a virus h1n1/product/Sino Biological
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant ha protein influenza a virus h1n1 - by Bioz Stars, 2021-07
    96/100 stars
      Buy from Supplier

    Image Search Results


    Overall experimental study design for T-cell epitope discovery in Pandemrix-associated NT1. As a first step, influenza A (H1N1) virus HA, NA, and NP peptide T cell recognition was tested with spleen cells from Pandemrix-vaccinated HLA-DQ6.2 mice, restimulated with pools of 5 peptides each (15-mers). Pools that stimulated IFN -γ or IL-2 expression were broken up, and single peptides tested either with spleen cells from additional Pandemrix-vaccinated HLA-DQ6.2 mice, or PBMC from Pandemrix-vaccinated HLA-DQB1*0602 positive individuals. As a second step, recognition of single peptides was then tested with PBMC from pediatric Pandemrix-associated NT1 patients, and healthy Pandemrix-vaccinated controls. As a third step, influenza A (H1N1) T cell peptides that showed increased stimulation of IFN - γ or IL-2 secretion in patients vs. controls were validated and mapped. Cross-reactive T cell self-epitopes were predicted by BLAST against human proteome, and recognition tested with PBMC from pediatric Pandemrix-associated NT1 patients, and healthy Pandemrix-vaccinated controls.

    Journal: Nature Communications

    Article Title: Enhanced influenza A H1N1 T cell epitope recognition and cross-reactivity to protein-O-mannosyltransferase 1 in Pandemrix-associated narcolepsy type 1

    doi: 10.1038/s41467-021-22637-8

    Figure Lengend Snippet: Overall experimental study design for T-cell epitope discovery in Pandemrix-associated NT1. As a first step, influenza A (H1N1) virus HA, NA, and NP peptide T cell recognition was tested with spleen cells from Pandemrix-vaccinated HLA-DQ6.2 mice, restimulated with pools of 5 peptides each (15-mers). Pools that stimulated IFN -γ or IL-2 expression were broken up, and single peptides tested either with spleen cells from additional Pandemrix-vaccinated HLA-DQ6.2 mice, or PBMC from Pandemrix-vaccinated HLA-DQB1*0602 positive individuals. As a second step, recognition of single peptides was then tested with PBMC from pediatric Pandemrix-associated NT1 patients, and healthy Pandemrix-vaccinated controls. As a third step, influenza A (H1N1) T cell peptides that showed increased stimulation of IFN - γ or IL-2 secretion in patients vs. controls were validated and mapped. Cross-reactive T cell self-epitopes were predicted by BLAST against human proteome, and recognition tested with PBMC from pediatric Pandemrix-associated NT1 patients, and healthy Pandemrix-vaccinated controls.

    Article Snippet: Peptides and recombinant proteins15-mer peptides covering hemagglutinin (GenBank entry ACP41953.1) and neuraminidase (YP_009118627.1) of influenza (A/California/07/2009 (H1N1)), and nucleoprotein (ADE29096.1) of influenza (A/reassortant/NYMC X-179A (California/07/2009 × NYMC X-157)(H1N1)) with 12 amino acid overlap were produced.

    Techniques: Mouse Assay, Expressing

    Influenza A H1N1 virus T-cell epitope screen in HLA-DQ6.2 mice, and Pandemrix-vaccinated HLA-DQB1*0602 positive individuals. Spleen cells from Pandemrix-immunized HLA-DQ6.2 mice were stimulated in culture with pools of five overlapping 15-mer peptides each, covering a hemagglutinin (HA), b neuraminidase (NA) or c nucleoprotein (NP) from influenza (A/reassortant/NYMC X-179A (California/07/2009 × NYMC X-157)(H1N1)) vaccine virus used in Pandemrix (pooling cells from 2 × 2 mice, n = 2). Recombinant hemagglutinin (rHA) and nucleoprotein (rNP; A/Puerto Rico/8/34 corresponding to the Pandemrix vaccine strain) were used as positive controls. d – f PBMC from Pandemrix-vaccinated HLA-DQB1*0602 positive individuals (sleep clinic patients without a diagnosis of NT1) were stimulated in culture with single 15-mer peptides, derived from the same vaccine virus strain ( n = 1–2). The expression of IFN-γ or IL-2 was measured by FMIA (protein) or RT-qPCR (mRNA). Results are expressed as ratios between cytokine concentrations (lines representing means) or relative gene expressions (dots representing single values, bars representing means) measured in peptide-stimulated and negative control samples (stimulation index). An asterisk (*) indicates a pool or single peptide that was selected for further testing.

    Journal: Nature Communications

    Article Title: Enhanced influenza A H1N1 T cell epitope recognition and cross-reactivity to protein-O-mannosyltransferase 1 in Pandemrix-associated narcolepsy type 1

    doi: 10.1038/s41467-021-22637-8

    Figure Lengend Snippet: Influenza A H1N1 virus T-cell epitope screen in HLA-DQ6.2 mice, and Pandemrix-vaccinated HLA-DQB1*0602 positive individuals. Spleen cells from Pandemrix-immunized HLA-DQ6.2 mice were stimulated in culture with pools of five overlapping 15-mer peptides each, covering a hemagglutinin (HA), b neuraminidase (NA) or c nucleoprotein (NP) from influenza (A/reassortant/NYMC X-179A (California/07/2009 × NYMC X-157)(H1N1)) vaccine virus used in Pandemrix (pooling cells from 2 × 2 mice, n = 2). Recombinant hemagglutinin (rHA) and nucleoprotein (rNP; A/Puerto Rico/8/34 corresponding to the Pandemrix vaccine strain) were used as positive controls. d – f PBMC from Pandemrix-vaccinated HLA-DQB1*0602 positive individuals (sleep clinic patients without a diagnosis of NT1) were stimulated in culture with single 15-mer peptides, derived from the same vaccine virus strain ( n = 1–2). The expression of IFN-γ or IL-2 was measured by FMIA (protein) or RT-qPCR (mRNA). Results are expressed as ratios between cytokine concentrations (lines representing means) or relative gene expressions (dots representing single values, bars representing means) measured in peptide-stimulated and negative control samples (stimulation index). An asterisk (*) indicates a pool or single peptide that was selected for further testing.

    Article Snippet: Peptides and recombinant proteins15-mer peptides covering hemagglutinin (GenBank entry ACP41953.1) and neuraminidase (YP_009118627.1) of influenza (A/California/07/2009 (H1N1)), and nucleoprotein (ADE29096.1) of influenza (A/reassortant/NYMC X-179A (California/07/2009 × NYMC X-157)(H1N1)) with 12 amino acid overlap were produced.

    Techniques: Mouse Assay, Recombinant, Derivative Assay, Expressing, Quantitative RT-PCR, Negative Control

    Mapping of identified influenza A H1N1 virus T-cell epitopes in Pandemrix-associated NT1 patients. a , d PBMC from pediatric Pandemrix-associated NT1 patients (NT1; validation cohort) or pediatric Pandemrix-vaccinated healthy controls (C) were stimulated in culture with overlapping 15-mer peptides from influenza (A/reassortant/NYMC X-179A (California/07/2009 × NYMC X-157)(H1N1)) vaccine virus neuraminidase (NA) or nucleoprotein (NP), as indicated. b , c , e , f PBMC from NT1 patients (invariably HLA-DQB1*0602 positive; discovery and validation cohorts combined; HLA-DQB1*0602 homozygous NT1 patients marked with red dots) or HLA-DQB1*0602 positive (C/DQ6+) or negative (C/DQ6−) healthy controls were stimulated with single NA- or NP-derived peptides. The secretion of IFN-γ was measured by FMIA (protein). Results are expressed as the ratio between cytokine concentrations measured in peptide-stimulated and negative control samples (stimulation index). Statistical comparisons between groups were performed, using Kruskal–Wallis and Dunn’s multiple comparisons tests.

    Journal: Nature Communications

    Article Title: Enhanced influenza A H1N1 T cell epitope recognition and cross-reactivity to protein-O-mannosyltransferase 1 in Pandemrix-associated narcolepsy type 1

    doi: 10.1038/s41467-021-22637-8

    Figure Lengend Snippet: Mapping of identified influenza A H1N1 virus T-cell epitopes in Pandemrix-associated NT1 patients. a , d PBMC from pediatric Pandemrix-associated NT1 patients (NT1; validation cohort) or pediatric Pandemrix-vaccinated healthy controls (C) were stimulated in culture with overlapping 15-mer peptides from influenza (A/reassortant/NYMC X-179A (California/07/2009 × NYMC X-157)(H1N1)) vaccine virus neuraminidase (NA) or nucleoprotein (NP), as indicated. b , c , e , f PBMC from NT1 patients (invariably HLA-DQB1*0602 positive; discovery and validation cohorts combined; HLA-DQB1*0602 homozygous NT1 patients marked with red dots) or HLA-DQB1*0602 positive (C/DQ6+) or negative (C/DQ6−) healthy controls were stimulated with single NA- or NP-derived peptides. The secretion of IFN-γ was measured by FMIA (protein). Results are expressed as the ratio between cytokine concentrations measured in peptide-stimulated and negative control samples (stimulation index). Statistical comparisons between groups were performed, using Kruskal–Wallis and Dunn’s multiple comparisons tests.

    Article Snippet: Peptides and recombinant proteins15-mer peptides covering hemagglutinin (GenBank entry ACP41953.1) and neuraminidase (YP_009118627.1) of influenza (A/California/07/2009 (H1N1)), and nucleoprotein (ADE29096.1) of influenza (A/reassortant/NYMC X-179A (California/07/2009 × NYMC X-157)(H1N1)) with 12 amino acid overlap were produced.

    Techniques: Derivative Assay, Negative Control

    Identification of influenza A H1N1 virus T-cell epitopes in Pandemrix-associated NT1 patients. PBMC from pediatric Pandemrix-associated NT1 patients (NT1) or pediatric Pandemrix-vaccinated healthy controls (C) were stimulated in culture with single 15-mer peptides from influenza (A/reassortant/NYMC X-179A (California/07/2009 × NYMC X-157)(H1N1)) vaccine virus hemagglutinin (HA), neuraminidase (NA) or nucleoprotein (NP) (discovery cohort). Recombinant neuraminidase (rNA) and nucleoprotein (rNP) were used as positive controls. The secretion of IFN-γ ( a , c , e ) or IL-2 ( b , d , f ) was measured by FMIA (protein). Results are expressed as the ratio between cytokine concentrations measured in peptide-stimulated and negative control samples (stimulation index). Statistical comparisons between groups were performed, using Kruskal–Wallis and Dunn’s multiple comparisons tests.

    Journal: Nature Communications

    Article Title: Enhanced influenza A H1N1 T cell epitope recognition and cross-reactivity to protein-O-mannosyltransferase 1 in Pandemrix-associated narcolepsy type 1

    doi: 10.1038/s41467-021-22637-8

    Figure Lengend Snippet: Identification of influenza A H1N1 virus T-cell epitopes in Pandemrix-associated NT1 patients. PBMC from pediatric Pandemrix-associated NT1 patients (NT1) or pediatric Pandemrix-vaccinated healthy controls (C) were stimulated in culture with single 15-mer peptides from influenza (A/reassortant/NYMC X-179A (California/07/2009 × NYMC X-157)(H1N1)) vaccine virus hemagglutinin (HA), neuraminidase (NA) or nucleoprotein (NP) (discovery cohort). Recombinant neuraminidase (rNA) and nucleoprotein (rNP) were used as positive controls. The secretion of IFN-γ ( a , c , e ) or IL-2 ( b , d , f ) was measured by FMIA (protein). Results are expressed as the ratio between cytokine concentrations measured in peptide-stimulated and negative control samples (stimulation index). Statistical comparisons between groups were performed, using Kruskal–Wallis and Dunn’s multiple comparisons tests.

    Article Snippet: Peptides and recombinant proteins15-mer peptides covering hemagglutinin (GenBank entry ACP41953.1) and neuraminidase (YP_009118627.1) of influenza (A/California/07/2009 (H1N1)), and nucleoprotein (ADE29096.1) of influenza (A/reassortant/NYMC X-179A (California/07/2009 × NYMC X-157)(H1N1)) with 12 amino acid overlap were produced.

    Techniques: Recombinant, Negative Control

    Effect of λ-CGN on influenza A infection in vivo . BALB/c mice were mock-infected (black) or intranasally infected with maPR8 at 5 MLD50 (red). As test groups, the virus was preincubated at room temperature for 30 min with λ-CGN at a lower dose (1 mg/kg/d, bright green) or a higher dose (5 mg/kg/d, dark green), followed by intranasal administration. Control mice received oral OSV-P twice a day (10 mg/kg/d) at 8-h intervals, starting at 4 h before viral infection (blue). Body weight (A) and mortality (B) of mice were measured every day from Days 0 to 14 post-infection. Data are expressed as the mean ± SEM from five mice

    Journal: bioRxiv

    Article Title: Antiviral activity of lambda-carrageenan against influenza viruses in mice and severe acute respiratory syndrome coronavirus 2 in vitro

    doi: 10.1101/2020.08.23.255364

    Figure Lengend Snippet: Effect of λ-CGN on influenza A infection in vivo . BALB/c mice were mock-infected (black) or intranasally infected with maPR8 at 5 MLD50 (red). As test groups, the virus was preincubated at room temperature for 30 min with λ-CGN at a lower dose (1 mg/kg/d, bright green) or a higher dose (5 mg/kg/d, dark green), followed by intranasal administration. Control mice received oral OSV-P twice a day (10 mg/kg/d) at 8-h intervals, starting at 4 h before viral infection (blue). Body weight (A) and mortality (B) of mice were measured every day from Days 0 to 14 post-infection. Data are expressed as the mean ± SEM from five mice

    Article Snippet: Viral NP and HA proteins were detected using mouse anti-NP (catalog no. 11675-MM03; Sino Biological, Beijing, China) and rabbit anti-HA2 (catalog no. 86001-RM01; Sino Biological) antibodies, respectively, according to our previous report .

    Techniques: Infection, In Vivo, Mouse Assay

    Effect of λ-CGN on the intracellular entry of influenza A virus. MDCK cells were infected with PR8 (MOI, 5) and subsequently mock-treated or treated either with λ-CGN or with p-KG03 at a concentration of 10 μg/ml. At 4 h p.i. in the absence of CHX (A) or at 2.5 h in the presence of 10 μg/ml CHX (B), viral NP was detected with an anti-NP antibody and an Alex Fluor 488-conjugated goat anti-mouse secondary antibody (green). Cell nuclei were counterstained with DAPI (blue). Original magnification, 400×.

    Journal: bioRxiv

    Article Title: Antiviral activity of lambda-carrageenan against influenza viruses in mice and severe acute respiratory syndrome coronavirus 2 in vitro

    doi: 10.1101/2020.08.23.255364

    Figure Lengend Snippet: Effect of λ-CGN on the intracellular entry of influenza A virus. MDCK cells were infected with PR8 (MOI, 5) and subsequently mock-treated or treated either with λ-CGN or with p-KG03 at a concentration of 10 μg/ml. At 4 h p.i. in the absence of CHX (A) or at 2.5 h in the presence of 10 μg/ml CHX (B), viral NP was detected with an anti-NP antibody and an Alex Fluor 488-conjugated goat anti-mouse secondary antibody (green). Cell nuclei were counterstained with DAPI (blue). Original magnification, 400×.

    Article Snippet: Viral NP and HA proteins were detected using mouse anti-NP (catalog no. 11675-MM03; Sino Biological, Beijing, China) and rabbit anti-HA2 (catalog no. 86001-RM01; Sino Biological) antibodies, respectively, according to our previous report .

    Techniques: Infection, Concentration Assay

    Construction and verification of mRNA vaccine encoding the H1N1-HA protein. ( a ) Agarose gel electrophoresis of  Xho I enzyme digestion products. 1 represents the intact plasmids of pGEM-H1N1-HA-n3 and 2 represents its linearized product of 5355 bp. M1: DL 5,000 DNA Marker (TaKaRa, Tokyo, Japan); M2: DL 2000 DNA Marker (TaKaRa, Tokyo, Japan). ( b,c ) Western blot and indirect immunofluorescence analyses. A549 cells were harvested 12 h and 48 h after transfection. The H1N1-HA protein was detected using rabbit anti-influenza A virus HA Mab. The H1N1-virus group was used as the positive control. Mock represents the negative control. DAPI was used to dye the nuclei.

    Journal: Vaccines

    Article Title: mRNA Vaccines Encoding the HA Protein of Influenza A H1N1 Virus Delivered by Cationic Lipid Nanoparticles Induce Protective Immune Responses in Mice

    doi: 10.3390/vaccines8010123

    Figure Lengend Snippet: Construction and verification of mRNA vaccine encoding the H1N1-HA protein. ( a ) Agarose gel electrophoresis of Xho I enzyme digestion products. 1 represents the intact plasmids of pGEM-H1N1-HA-n3 and 2 represents its linearized product of 5355 bp. M1: DL 5,000 DNA Marker (TaKaRa, Tokyo, Japan); M2: DL 2000 DNA Marker (TaKaRa, Tokyo, Japan). ( b,c ) Western blot and indirect immunofluorescence analyses. A549 cells were harvested 12 h and 48 h after transfection. The H1N1-HA protein was detected using rabbit anti-influenza A virus HA Mab. The H1N1-virus group was used as the positive control. Mock represents the negative control. DAPI was used to dye the nuclei.

    Article Snippet: The same amount of protein was taken for SDS-polyacrylamide gel electrophoresis separation, transferred on to the nitrocellulose membrane (GE Healthcare, Little Chalfont, Buckinghamshire, UK), blocked with 5% skimmed milk, mixed with influenza A virus HA rabbit Mab (1:2000) (Sino Biological, Beijing, China) at room temperature for 2 h, and washed thrice with TBST for 8 min each.

    Techniques: Agarose Gel Electrophoresis, Marker, Western Blot, Immunofluorescence, Transfection, Positive Control, Negative Control

    Antiviral activity of ginseng extract (GE) against 2009 pandemic H1N1 influenza A virus infection. (A) A dose dependent reduction in viral titer was observed when MDCK cells were infected with GE-pretreated A/Nanchang/8002/2009 (H1N1) virus. Protective effect of GE was observed on (B) weight loss and (C) survival of mice infected with A/Nanchang/8002/2009 (H1N1) virus. GE oral_ mice treated with 80mg/kg of GE by oral route. GE_ mice were infected with a mixture of 10 3 EID 50 of virus and GE. Mock_ there was no GE treatment given. *** P

    Journal: PLoS ONE

    Article Title: Inhibition of influenza A virus infection by ginsenosides

    doi: 10.1371/journal.pone.0171936

    Figure Lengend Snippet: Antiviral activity of ginseng extract (GE) against 2009 pandemic H1N1 influenza A virus infection. (A) A dose dependent reduction in viral titer was observed when MDCK cells were infected with GE-pretreated A/Nanchang/8002/2009 (H1N1) virus. Protective effect of GE was observed on (B) weight loss and (C) survival of mice infected with A/Nanchang/8002/2009 (H1N1) virus. GE oral_ mice treated with 80mg/kg of GE by oral route. GE_ mice were infected with a mixture of 10 3 EID 50 of virus and GE. Mock_ there was no GE treatment given. *** P

    Article Snippet: Immunoblot assayDifferent concentrations of ginsenosides Rb1 were mixed with recombinant HA protein -Influenza A virus H1N1 (A/California/07/2009) (Sino Biological Inc., Beijing, China) and incubated at 37°C for 1 hour and then was spotted onto PVDF membrane.

    Techniques: Activity Assay, Infection, Mouse Assay