recombinant gp120  (Sino Biological)


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    Human Immunodeficiency Virus type 1 gp120 Glycoprotein 120 ELISA Pair Set
    Description:
    Each vial contains 165 ng of recombinant Human Immunodeficiency Virus type 1 HIV 1 gp120 Glycoprotein 120 Reconstitute with 1 mL detection antibody dilution buffer After reconstitution store at 20℃ to 80℃ in a manual defrost freezer A seven point standard curve using 2 fold serial dilutions in sample dilution buffer and a high standard of 3000 pg mL is recommended
    Catalog Number:
    SEK11233
    Price:
    None
    Category:
    Elisa pair set
    Reactivity:
    HIV
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    Structured Review

    Sino Biological recombinant gp120
    Activation of <t>gp120-primed</t> DC reduced the expression of Bcl2 and activated Akt, and the induction of cell apoptosis is ASK1-dependent. ( A ) moDC treated with immune-complex gp120 ADA were exposed to CL40L Tf or mock Tf, or LPS, TNFα, IL-1β for 3 d, and cellular proteins of moDC (recovered from coculture) were extracted for Western blotting analysis. MoDC treated by anti-His Ab (used to cross-link recombinant gp120 ADA ) were used as a control (control DC). *p-ASK1 represent results from untreated moDC (lane 1) and DC treated as indicated but no gp120 pulsing (lanes 2–6), whereas #p-ASK-1 represents results from pre-treatment of anti-DC-SIGN mAbs. Data are representative of 3 experiments. ( B ) moDC were transfected with siRNA against human ASK1 or control siRNA (scrambled) before pulse with immune-complex gp120 and subsequent exposure to CD40L Tf, LPS, TNFα or IL-1β. Data are expressed as mean ± SD from 3 experiments. **p
    Each vial contains 165 ng of recombinant Human Immunodeficiency Virus type 1 HIV 1 gp120 Glycoprotein 120 Reconstitute with 1 mL detection antibody dilution buffer After reconstitution store at 20℃ to 80℃ in a manual defrost freezer A seven point standard curve using 2 fold serial dilutions in sample dilution buffer and a high standard of 3000 pg mL is recommended
    https://www.bioz.com/result/recombinant gp120/product/Sino Biological
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant gp120 - by Bioz Stars, 2021-06
    91/100 stars

    Images

    1) Product Images from "Binding of HIV-1 gp120 to DC-SIGN Promotes ASK-1-Dependent Activation-Induced Apoptosis of Human Dendritic Cells"

    Article Title: Binding of HIV-1 gp120 to DC-SIGN Promotes ASK-1-Dependent Activation-Induced Apoptosis of Human Dendritic Cells

    Journal: PLoS Pathogens

    doi: 10.1371/journal.ppat.1003100

    Activation of gp120-primed DC reduced the expression of Bcl2 and activated Akt, and the induction of cell apoptosis is ASK1-dependent. ( A ) moDC treated with immune-complex gp120 ADA were exposed to CL40L Tf or mock Tf, or LPS, TNFα, IL-1β for 3 d, and cellular proteins of moDC (recovered from coculture) were extracted for Western blotting analysis. MoDC treated by anti-His Ab (used to cross-link recombinant gp120 ADA ) were used as a control (control DC). *p-ASK1 represent results from untreated moDC (lane 1) and DC treated as indicated but no gp120 pulsing (lanes 2–6), whereas #p-ASK-1 represents results from pre-treatment of anti-DC-SIGN mAbs. Data are representative of 3 experiments. ( B ) moDC were transfected with siRNA against human ASK1 or control siRNA (scrambled) before pulse with immune-complex gp120 and subsequent exposure to CD40L Tf, LPS, TNFα or IL-1β. Data are expressed as mean ± SD from 3 experiments. **p
    Figure Legend Snippet: Activation of gp120-primed DC reduced the expression of Bcl2 and activated Akt, and the induction of cell apoptosis is ASK1-dependent. ( A ) moDC treated with immune-complex gp120 ADA were exposed to CL40L Tf or mock Tf, or LPS, TNFα, IL-1β for 3 d, and cellular proteins of moDC (recovered from coculture) were extracted for Western blotting analysis. MoDC treated by anti-His Ab (used to cross-link recombinant gp120 ADA ) were used as a control (control DC). *p-ASK1 represent results from untreated moDC (lane 1) and DC treated as indicated but no gp120 pulsing (lanes 2–6), whereas #p-ASK-1 represents results from pre-treatment of anti-DC-SIGN mAbs. Data are representative of 3 experiments. ( B ) moDC were transfected with siRNA against human ASK1 or control siRNA (scrambled) before pulse with immune-complex gp120 and subsequent exposure to CD40L Tf, LPS, TNFα or IL-1β. Data are expressed as mean ± SD from 3 experiments. **p

    Techniques Used: Activation Assay, Expressing, Western Blot, Recombinant, Transfection

    Cross-linked gp120 sensitizes DC through DC-SIGN and MCLRs for CD40L-mediated apoptosis. ( A , B ) moDC were pretreated with anti-DC-SIGN mAbs or isotype control Ab before pulse with cross-linked gp120 ADA and co-culture for 3 d with autologous activated CD4 T cells, and subsequently subjected to cell viability assay. Data are representative of 3 experiments and are expressed as mean ± SD from 3 experiments in B . ( C, D ) moDC were treated with cross-linked recombinant gp120 ADA with or without pre-treatment by soluble ICAM-3-Fc chimeric protein, anti-CD4 plus anti-CCR5 mAbs, or anti-DC-SIGN mAbs. Cells were subsequently co-cultured with mock- or CD40L-transfected (CD40L Tf) cells for 3 d. Data are representative of 7 experiments in panel C and are expressed as mean ± SD (n = 7) in D ; ***p
    Figure Legend Snippet: Cross-linked gp120 sensitizes DC through DC-SIGN and MCLRs for CD40L-mediated apoptosis. ( A , B ) moDC were pretreated with anti-DC-SIGN mAbs or isotype control Ab before pulse with cross-linked gp120 ADA and co-culture for 3 d with autologous activated CD4 T cells, and subsequently subjected to cell viability assay. Data are representative of 3 experiments and are expressed as mean ± SD from 3 experiments in B . ( C, D ) moDC were treated with cross-linked recombinant gp120 ADA with or without pre-treatment by soluble ICAM-3-Fc chimeric protein, anti-CD4 plus anti-CCR5 mAbs, or anti-DC-SIGN mAbs. Cells were subsequently co-cultured with mock- or CD40L-transfected (CD40L Tf) cells for 3 d. Data are representative of 7 experiments in panel C and are expressed as mean ± SD (n = 7) in D ; ***p

    Techniques Used: Co-Culture Assay, Viability Assay, Recombinant, Cell Culture, Transfection

    Sera from HIV-1(+) individuals can sensitize moDC for DC-SIGN dependent CD40L-mediated apoptosis. ( A ) moDC were treated with HIV(+) serum before or after immunoprecipitation (IP) with anti-gp120 mAbs, or with or without anti-DC-SIGN or isotype control mAbs, and subsequently co-cultured with autologous activated CD4 T cells. After 3 d, cells were harvested and subjected to TUNEL assays. Cell death was assessed as the percentage of cells expressing terminal deoxynucleotidyl transferase (TdT). DC pulsed with HIV(+) serum without coculture with activated CD4 T cells (top panel) were also used as a control. Data are representative of 4 experiments. ( B ) MoDCs were treated with anti-DC-SIGN mAbs, isotype control Ab, or anti-CD40L mAb before pulse with HIV serum (before or after immunoprecipitation of gp120) and cocultured with activated CD4 T cells. Data are expressed as mean ± SD (n = 4); *p
    Figure Legend Snippet: Sera from HIV-1(+) individuals can sensitize moDC for DC-SIGN dependent CD40L-mediated apoptosis. ( A ) moDC were treated with HIV(+) serum before or after immunoprecipitation (IP) with anti-gp120 mAbs, or with or without anti-DC-SIGN or isotype control mAbs, and subsequently co-cultured with autologous activated CD4 T cells. After 3 d, cells were harvested and subjected to TUNEL assays. Cell death was assessed as the percentage of cells expressing terminal deoxynucleotidyl transferase (TdT). DC pulsed with HIV(+) serum without coculture with activated CD4 T cells (top panel) were also used as a control. Data are representative of 4 experiments. ( B ) MoDCs were treated with anti-DC-SIGN mAbs, isotype control Ab, or anti-CD40L mAb before pulse with HIV serum (before or after immunoprecipitation of gp120) and cocultured with activated CD4 T cells. Data are expressed as mean ± SD (n = 4); *p

    Techniques Used: Immunoprecipitation, Cell Culture, TUNEL Assay, Expressing

    Cross-linked recombinant gp120 sensitizes moDC for CD40L-mediated apoptosis after co-culture with activated CD4 T cells. ( A ) moDC were treated for 24 h with anti-His mAb alone (control DC, left panels) or with 25 nM gp120 ADA cross-linked with anti-His mAb (gp120-DC, right panels), and co-cultured with autologous activated (upper panels) or naïve (lower panels) CD4 T cells for 3 d. The moDC ( Fig. S1 ) were analyzed for Annexin V (AV) and propidium iodide (PI) expression to assess the extent of apoptosis, as manifested by the percentage of the AV-positive [AV(+)] cells. Data are representative of 5 experiments. ( B ) Apoptosis of moDC was analyzed after treatment with different concentrations of cross-linked recombinant gp120 ADA or gp120 HXBc2 and co-culture with activated CD4 T cells for 3 d. DC treated with monomeric gp120 (not cross-linked with anti-His or anti-FLAG mAb) were used as a control. Data represent mean ± SD from 5 experiments; **p
    Figure Legend Snippet: Cross-linked recombinant gp120 sensitizes moDC for CD40L-mediated apoptosis after co-culture with activated CD4 T cells. ( A ) moDC were treated for 24 h with anti-His mAb alone (control DC, left panels) or with 25 nM gp120 ADA cross-linked with anti-His mAb (gp120-DC, right panels), and co-cultured with autologous activated (upper panels) or naïve (lower panels) CD4 T cells for 3 d. The moDC ( Fig. S1 ) were analyzed for Annexin V (AV) and propidium iodide (PI) expression to assess the extent of apoptosis, as manifested by the percentage of the AV-positive [AV(+)] cells. Data are representative of 5 experiments. ( B ) Apoptosis of moDC was analyzed after treatment with different concentrations of cross-linked recombinant gp120 ADA or gp120 HXBc2 and co-culture with activated CD4 T cells for 3 d. DC treated with monomeric gp120 (not cross-linked with anti-His or anti-FLAG mAb) were used as a control. Data represent mean ± SD from 5 experiments; **p

    Techniques Used: Recombinant, Co-Culture Assay, Cell Culture, Expressing

    Cross-linked recombinant gp120 or HIV(+) serum sensitizes moDC for apoptosis after activation by LPS, TNF-α or IL-1β, and DC-SIGN(+) cells in HIV(+) blood are pre-sensitized for LPS/TNFα/IL-1β-induced apoptosis. ( A,B ) moDC were treated with gp120 ADA in the presence of isotype control or anti-DC-SIGN Abs and subsequently cultured in the absence or presence of 100 ng/ml LPS for 3 d. Data are representative of 5 experiments in A and are expressed as mean ± SD in B ; **p
    Figure Legend Snippet: Cross-linked recombinant gp120 or HIV(+) serum sensitizes moDC for apoptosis after activation by LPS, TNF-α or IL-1β, and DC-SIGN(+) cells in HIV(+) blood are pre-sensitized for LPS/TNFα/IL-1β-induced apoptosis. ( A,B ) moDC were treated with gp120 ADA in the presence of isotype control or anti-DC-SIGN Abs and subsequently cultured in the absence or presence of 100 ng/ml LPS for 3 d. Data are representative of 5 experiments in A and are expressed as mean ± SD in B ; **p

    Techniques Used: Recombinant, Activation Assay, Cell Culture

    Freshly-isolated DC-SIGN(+) blood DC underwent DC-SIGN-dependent CD40L-mediated apoptosis and DC-SIGN(+) cells from HIV-1-infected individuals are pre-sensitized for CD40L-mediated apoptosis. ( A ) PBMCs from normal HIV(−) individuals were labelled with anti-CD14 plus either isotype control (left panel) or anti-DC-SIGN (right panel) mAbs and analysed by flow cytometry for cell isolation. Data are representative of 4 experiments. ( B ) Purified CD14(+)DC-SIGN(+) cells were treated with anti-His mAb alone (Control) or anti-His cross-linked recombinant gp120 ADA , in the absence or presence of anti-DC-SIGN mAbs, and subsequently co-cultured with CD40L Tf for 3 d. The non-adherent DC were then harvested and subjected to cell viability assay. Data are representative of 4 experiments. ( C, D ) freshly isolated DC-SIGN(+) cells from HIV(+) and HIV(−) blood were cocultured with mock Tf or CD40L Tf for 3 days and subjected to cell viability assay. Data are representative of 4 experiments in C and expressed as mean ± SD in D . ***p
    Figure Legend Snippet: Freshly-isolated DC-SIGN(+) blood DC underwent DC-SIGN-dependent CD40L-mediated apoptosis and DC-SIGN(+) cells from HIV-1-infected individuals are pre-sensitized for CD40L-mediated apoptosis. ( A ) PBMCs from normal HIV(−) individuals were labelled with anti-CD14 plus either isotype control (left panel) or anti-DC-SIGN (right panel) mAbs and analysed by flow cytometry for cell isolation. Data are representative of 4 experiments. ( B ) Purified CD14(+)DC-SIGN(+) cells were treated with anti-His mAb alone (Control) or anti-His cross-linked recombinant gp120 ADA , in the absence or presence of anti-DC-SIGN mAbs, and subsequently co-cultured with CD40L Tf for 3 d. The non-adherent DC were then harvested and subjected to cell viability assay. Data are representative of 4 experiments. ( C, D ) freshly isolated DC-SIGN(+) cells from HIV(+) and HIV(−) blood were cocultured with mock Tf or CD40L Tf for 3 days and subjected to cell viability assay. Data are representative of 4 experiments in C and expressed as mean ± SD in D . ***p

    Techniques Used: Isolation, Infection, Flow Cytometry, Cytometry, Cell Isolation, Purification, Recombinant, Cell Culture, Viability Assay

    2) Product Images from "Binding of HIV-1 gp120 to DC-SIGN Promotes ASK-1-Dependent Activation-Induced Apoptosis of Human Dendritic Cells"

    Article Title: Binding of HIV-1 gp120 to DC-SIGN Promotes ASK-1-Dependent Activation-Induced Apoptosis of Human Dendritic Cells

    Journal: PLoS Pathogens

    doi: 10.1371/journal.ppat.1003100

    Activation of gp120-primed DC reduced the expression of Bcl2 and activated Akt, and the induction of cell apoptosis is ASK1-dependent. ( A ) moDC treated with immune-complex gp120 ADA were exposed to CL40L Tf or mock Tf, or LPS, TNFα, IL-1β for 3 d, and cellular proteins of moDC (recovered from coculture) were extracted for Western blotting analysis. MoDC treated by anti-His Ab (used to cross-link recombinant gp120 ADA ) were used as a control (control DC). *p-ASK1 represent results from untreated moDC (lane 1) and DC treated as indicated but no gp120 pulsing (lanes 2–6), whereas #p-ASK-1 represents results from pre-treatment of anti-DC-SIGN mAbs. Data are representative of 3 experiments. ( B ) moDC were transfected with siRNA against human ASK1 or control siRNA (scrambled) before pulse with immune-complex gp120 and subsequent exposure to CD40L Tf, LPS, TNFα or IL-1β. Data are expressed as mean ± SD from 3 experiments. **p
    Figure Legend Snippet: Activation of gp120-primed DC reduced the expression of Bcl2 and activated Akt, and the induction of cell apoptosis is ASK1-dependent. ( A ) moDC treated with immune-complex gp120 ADA were exposed to CL40L Tf or mock Tf, or LPS, TNFα, IL-1β for 3 d, and cellular proteins of moDC (recovered from coculture) were extracted for Western blotting analysis. MoDC treated by anti-His Ab (used to cross-link recombinant gp120 ADA ) were used as a control (control DC). *p-ASK1 represent results from untreated moDC (lane 1) and DC treated as indicated but no gp120 pulsing (lanes 2–6), whereas #p-ASK-1 represents results from pre-treatment of anti-DC-SIGN mAbs. Data are representative of 3 experiments. ( B ) moDC were transfected with siRNA against human ASK1 or control siRNA (scrambled) before pulse with immune-complex gp120 and subsequent exposure to CD40L Tf, LPS, TNFα or IL-1β. Data are expressed as mean ± SD from 3 experiments. **p

    Techniques Used: Activation Assay, Expressing, Western Blot, Recombinant, Transfection

    Cross-linked gp120 sensitizes DC through DC-SIGN and MCLRs for CD40L-mediated apoptosis. ( A , B ) moDC were pretreated with anti-DC-SIGN mAbs or isotype control Ab before pulse with cross-linked gp120 ADA and co-culture for 3 d with autologous activated CD4 T cells, and subsequently subjected to cell viability assay. Data are representative of 3 experiments and are expressed as mean ± SD from 3 experiments in B . ( C, D ) moDC were treated with cross-linked recombinant gp120 ADA with or without pre-treatment by soluble ICAM-3-Fc chimeric protein, anti-CD4 plus anti-CCR5 mAbs, or anti-DC-SIGN mAbs. Cells were subsequently co-cultured with mock- or CD40L-transfected (CD40L Tf) cells for 3 d. Data are representative of 7 experiments in panel C and are expressed as mean ± SD (n = 7) in D ; ***p
    Figure Legend Snippet: Cross-linked gp120 sensitizes DC through DC-SIGN and MCLRs for CD40L-mediated apoptosis. ( A , B ) moDC were pretreated with anti-DC-SIGN mAbs or isotype control Ab before pulse with cross-linked gp120 ADA and co-culture for 3 d with autologous activated CD4 T cells, and subsequently subjected to cell viability assay. Data are representative of 3 experiments and are expressed as mean ± SD from 3 experiments in B . ( C, D ) moDC were treated with cross-linked recombinant gp120 ADA with or without pre-treatment by soluble ICAM-3-Fc chimeric protein, anti-CD4 plus anti-CCR5 mAbs, or anti-DC-SIGN mAbs. Cells were subsequently co-cultured with mock- or CD40L-transfected (CD40L Tf) cells for 3 d. Data are representative of 7 experiments in panel C and are expressed as mean ± SD (n = 7) in D ; ***p

    Techniques Used: Co-Culture Assay, Viability Assay, Recombinant, Cell Culture, Transfection

    Sera from HIV-1(+) individuals can sensitize moDC for DC-SIGN dependent CD40L-mediated apoptosis. ( A ) moDC were treated with HIV(+) serum before or after immunoprecipitation (IP) with anti-gp120 mAbs, or with or without anti-DC-SIGN or isotype control mAbs, and subsequently co-cultured with autologous activated CD4 T cells. After 3 d, cells were harvested and subjected to TUNEL assays. Cell death was assessed as the percentage of cells expressing terminal deoxynucleotidyl transferase (TdT). DC pulsed with HIV(+) serum without coculture with activated CD4 T cells (top panel) were also used as a control. Data are representative of 4 experiments. ( B ) MoDCs were treated with anti-DC-SIGN mAbs, isotype control Ab, or anti-CD40L mAb before pulse with HIV serum (before or after immunoprecipitation of gp120) and cocultured with activated CD4 T cells. Data are expressed as mean ± SD (n = 4); *p
    Figure Legend Snippet: Sera from HIV-1(+) individuals can sensitize moDC for DC-SIGN dependent CD40L-mediated apoptosis. ( A ) moDC were treated with HIV(+) serum before or after immunoprecipitation (IP) with anti-gp120 mAbs, or with or without anti-DC-SIGN or isotype control mAbs, and subsequently co-cultured with autologous activated CD4 T cells. After 3 d, cells were harvested and subjected to TUNEL assays. Cell death was assessed as the percentage of cells expressing terminal deoxynucleotidyl transferase (TdT). DC pulsed with HIV(+) serum without coculture with activated CD4 T cells (top panel) were also used as a control. Data are representative of 4 experiments. ( B ) MoDCs were treated with anti-DC-SIGN mAbs, isotype control Ab, or anti-CD40L mAb before pulse with HIV serum (before or after immunoprecipitation of gp120) and cocultured with activated CD4 T cells. Data are expressed as mean ± SD (n = 4); *p

    Techniques Used: Immunoprecipitation, Cell Culture, TUNEL Assay, Expressing

    Cross-linked recombinant gp120 sensitizes moDC for CD40L-mediated apoptosis after co-culture with activated CD4 T cells. ( A ) moDC were treated for 24 h with anti-His mAb alone (control DC, left panels) or with 25 nM gp120 ADA cross-linked with anti-His mAb (gp120-DC, right panels), and co-cultured with autologous activated (upper panels) or naïve (lower panels) CD4 T cells for 3 d. The moDC ( Fig. S1 ) were analyzed for Annexin V (AV) and propidium iodide (PI) expression to assess the extent of apoptosis, as manifested by the percentage of the AV-positive [AV(+)] cells. Data are representative of 5 experiments. ( B ) Apoptosis of moDC was analyzed after treatment with different concentrations of cross-linked recombinant gp120 ADA or gp120 HXBc2 and co-culture with activated CD4 T cells for 3 d. DC treated with monomeric gp120 (not cross-linked with anti-His or anti-FLAG mAb) were used as a control. Data represent mean ± SD from 5 experiments; **p
    Figure Legend Snippet: Cross-linked recombinant gp120 sensitizes moDC for CD40L-mediated apoptosis after co-culture with activated CD4 T cells. ( A ) moDC were treated for 24 h with anti-His mAb alone (control DC, left panels) or with 25 nM gp120 ADA cross-linked with anti-His mAb (gp120-DC, right panels), and co-cultured with autologous activated (upper panels) or naïve (lower panels) CD4 T cells for 3 d. The moDC ( Fig. S1 ) were analyzed for Annexin V (AV) and propidium iodide (PI) expression to assess the extent of apoptosis, as manifested by the percentage of the AV-positive [AV(+)] cells. Data are representative of 5 experiments. ( B ) Apoptosis of moDC was analyzed after treatment with different concentrations of cross-linked recombinant gp120 ADA or gp120 HXBc2 and co-culture with activated CD4 T cells for 3 d. DC treated with monomeric gp120 (not cross-linked with anti-His or anti-FLAG mAb) were used as a control. Data represent mean ± SD from 5 experiments; **p

    Techniques Used: Recombinant, Co-Culture Assay, Cell Culture, Expressing

    Cross-linked recombinant gp120 or HIV(+) serum sensitizes moDC for apoptosis after activation by LPS, TNF-α or IL-1β, and DC-SIGN(+) cells in HIV(+) blood are pre-sensitized for LPS/TNFα/IL-1β-induced apoptosis. ( A,B ) moDC were treated with gp120 ADA in the presence of isotype control or anti-DC-SIGN Abs and subsequently cultured in the absence or presence of 100 ng/ml LPS for 3 d. Data are representative of 5 experiments in A and are expressed as mean ± SD in B ; **p
    Figure Legend Snippet: Cross-linked recombinant gp120 or HIV(+) serum sensitizes moDC for apoptosis after activation by LPS, TNF-α or IL-1β, and DC-SIGN(+) cells in HIV(+) blood are pre-sensitized for LPS/TNFα/IL-1β-induced apoptosis. ( A,B ) moDC were treated with gp120 ADA in the presence of isotype control or anti-DC-SIGN Abs and subsequently cultured in the absence or presence of 100 ng/ml LPS for 3 d. Data are representative of 5 experiments in A and are expressed as mean ± SD in B ; **p

    Techniques Used: Recombinant, Activation Assay, Cell Culture

    Freshly-isolated DC-SIGN(+) blood DC underwent DC-SIGN-dependent CD40L-mediated apoptosis and DC-SIGN(+) cells from HIV-1-infected individuals are pre-sensitized for CD40L-mediated apoptosis. ( A ) PBMCs from normal HIV(−) individuals were labelled with anti-CD14 plus either isotype control (left panel) or anti-DC-SIGN (right panel) mAbs and analysed by flow cytometry for cell isolation. Data are representative of 4 experiments. ( B ) Purified CD14(+)DC-SIGN(+) cells were treated with anti-His mAb alone (Control) or anti-His cross-linked recombinant gp120 ADA , in the absence or presence of anti-DC-SIGN mAbs, and subsequently co-cultured with CD40L Tf for 3 d. The non-adherent DC were then harvested and subjected to cell viability assay. Data are representative of 4 experiments. ( C, D ) freshly isolated DC-SIGN(+) cells from HIV(+) and HIV(−) blood were cocultured with mock Tf or CD40L Tf for 3 days and subjected to cell viability assay. Data are representative of 4 experiments in C and expressed as mean ± SD in D . ***p
    Figure Legend Snippet: Freshly-isolated DC-SIGN(+) blood DC underwent DC-SIGN-dependent CD40L-mediated apoptosis and DC-SIGN(+) cells from HIV-1-infected individuals are pre-sensitized for CD40L-mediated apoptosis. ( A ) PBMCs from normal HIV(−) individuals were labelled with anti-CD14 plus either isotype control (left panel) or anti-DC-SIGN (right panel) mAbs and analysed by flow cytometry for cell isolation. Data are representative of 4 experiments. ( B ) Purified CD14(+)DC-SIGN(+) cells were treated with anti-His mAb alone (Control) or anti-His cross-linked recombinant gp120 ADA , in the absence or presence of anti-DC-SIGN mAbs, and subsequently co-cultured with CD40L Tf for 3 d. The non-adherent DC were then harvested and subjected to cell viability assay. Data are representative of 4 experiments. ( C, D ) freshly isolated DC-SIGN(+) cells from HIV(+) and HIV(−) blood were cocultured with mock Tf or CD40L Tf for 3 days and subjected to cell viability assay. Data are representative of 4 experiments in C and expressed as mean ± SD in D . ***p

    Techniques Used: Isolation, Infection, Flow Cytometry, Cell Isolation, Purification, Recombinant, Cell Culture, Viability Assay

    Related Articles

    Recombinant:

    Article Title: Binding of HIV-1 gp120 to DC-SIGN Promotes ASK-1-Dependent Activation-Induced Apoptosis of Human Dendritic Cells
    Article Snippet: This finding is in line with results of (of the text) and indicates that as incubation time increases, some cells at early apoptotic (AV+PI−) phase would become late apoptotic (AV+PI+) cells. are representative of 3 experiments. (TIF). .. EndoH treatment of recombinant gp120 abolished its binding to moDC. (A ) Recombinant gp120ADA supernatant was treated overnight with 25 KU of EndoH/ml as described (Hong PWP et al, J Virol 2002;76:12855–12865), and the treated and the untreated gp120ADA supernatant were subjected to Western blot assay by polyclonal rabbit anti-gp120 Ab (Sino Biological Inc). .. Figure S2 EndoH treatment of recombinant gp120 abolished its binding to moDC. (A ) Recombinant gp120ADA supernatant was treated overnight with 25 KU of EndoH/ml as described (Hong PWP et al, J Virol 2002;76:12855–12865), and the treated and the untreated gp120ADA supernatant were subjected to Western blot assay by polyclonal rabbit anti-gp120 Ab (Sino Biological Inc).

    Article Title: 3-Hydroxyphthalic Anhydride- Modified Rabbit Anti-PAP IgG as a Potential Bifunctional HIV-1 Entry Inhibitor
    Article Snippet: HP, polyethyleneimine (PEI), biotin goat-anti-rabbit IgG, bovine serum albumin (BSA), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), dimethyl sulfoxide (DMSO), thioflavin T (ThT), Congo Red Kits, biotinylated goat anti-rabbit IgG and FITC-goat-anti-rabbit-IgG were purchased from Sigma-Aldrich (St. Louis, MO, United States). .. The recombinant human CD4 protein and HIV-1JR-FL gp120 were purchased from Sino Biological (Wayne, PA, United States). .. The pNL4-3E-R-Luc plasmid, HIV-1 and VSV-G Env-encoding plasmids, the peGFP-Vpr plasmid, MT-2 cells, TZM-bl cells, CHO-WT cells, U87-CD4-CCR5 cells, U87-CD4-CXCR4 cells, HIV-1IIIB-infected H9 cells (H9/HIV-1IIIB), laboratory-adapted and primary HIV-1 strains, HL2/3 cells, HeLa cells, HeLa-CD4-LTR-β-gal cells, T20, AZT, AMD3100, Maraviroc, anti-p24 monoclonal antibody (183-12H-5C) and HIV immunoglobulin (HIV-IgG) were obtained from the National Institutes of Health AIDS Research and Reference Reagent Program.

    Article Title: Binding of HIV-1 gp120 to DC-SIGN Promotes ASK-1-Dependent Activation-Induced Apoptosis of Human Dendritic Cells
    Article Snippet: EndoH treatment of recombinant gp120 abolished its binding to moDC. (A ) Recombinant gp120ADA supernatant was treated overnight with 25 KU of EndoH/ml as described (Hong PWP et al, J Virol 2002;76:12855–12865), and the treated and the untreated gp120ADA supernatant were subjected to Western blot assay by polyclonal rabbit anti-gp120 Ab (Sino Biological Inc). .. Figure S2 EndoH treatment of recombinant gp120 abolished its binding to moDC. (A ) Recombinant gp120ADA supernatant was treated overnight with 25 KU of EndoH/ml as described (Hong PWP et al, J Virol 2002;76:12855–12865), and the treated and the untreated gp120ADA supernatant were subjected to Western blot assay by polyclonal rabbit anti-gp120 Ab (Sino Biological Inc). .. The untreated gp120 had a MW≈120 kDa, whereas the EndoH-treated one had MW≈80 kDa. (B ) EndoH-untreated recombinant gp120ADA and gp120HXBc2 were cross-linked with anti-His or anti-FLAG Ab, as described in materials and methods, and subsequently subjected to native non-reducing Western blot analysis (as described by Hong PWP et al, J Virol 2002;76:12855–12865).

    Binding Assay:

    Article Title: Binding of HIV-1 gp120 to DC-SIGN Promotes ASK-1-Dependent Activation-Induced Apoptosis of Human Dendritic Cells
    Article Snippet: This finding is in line with results of (of the text) and indicates that as incubation time increases, some cells at early apoptotic (AV+PI−) phase would become late apoptotic (AV+PI+) cells. are representative of 3 experiments. (TIF). .. EndoH treatment of recombinant gp120 abolished its binding to moDC. (A ) Recombinant gp120ADA supernatant was treated overnight with 25 KU of EndoH/ml as described (Hong PWP et al, J Virol 2002;76:12855–12865), and the treated and the untreated gp120ADA supernatant were subjected to Western blot assay by polyclonal rabbit anti-gp120 Ab (Sino Biological Inc). .. Figure S2 EndoH treatment of recombinant gp120 abolished its binding to moDC. (A ) Recombinant gp120ADA supernatant was treated overnight with 25 KU of EndoH/ml as described (Hong PWP et al, J Virol 2002;76:12855–12865), and the treated and the untreated gp120ADA supernatant were subjected to Western blot assay by polyclonal rabbit anti-gp120 Ab (Sino Biological Inc).

    Article Title: An autoreactive antibody from an SLE/HIV-1 individual broadly neutralizes HIV-1
    Article Snippet: Lot-specific protein spot definitions were provided by the microchip manufacturer and aligned to the image. .. A panel of 32 HIV-1 YU2 gp120 core mutants displayed on S. cerevisiae ( ) was probed for binding to CD4bs mAbs and CD4-Fc (Sino Biological). .. For each core mutant, 600,000 induced yeast cells were incubated with anti-HA Ab clone 16B12 (Covance) to detect expression and CD4bs mAbs or CD4-Fc in 50 μl PBSB in black 96-well plates (Greiner Bio One) for 1 hour at room temperature while shaking.

    Article Title: Binding of HIV-1 gp120 to DC-SIGN Promotes ASK-1-Dependent Activation-Induced Apoptosis of Human Dendritic Cells
    Article Snippet: EndoH treatment of recombinant gp120 abolished its binding to moDC. (A ) Recombinant gp120ADA supernatant was treated overnight with 25 KU of EndoH/ml as described (Hong PWP et al, J Virol 2002;76:12855–12865), and the treated and the untreated gp120ADA supernatant were subjected to Western blot assay by polyclonal rabbit anti-gp120 Ab (Sino Biological Inc). .. Figure S2 EndoH treatment of recombinant gp120 abolished its binding to moDC. (A ) Recombinant gp120ADA supernatant was treated overnight with 25 KU of EndoH/ml as described (Hong PWP et al, J Virol 2002;76:12855–12865), and the treated and the untreated gp120ADA supernatant were subjected to Western blot assay by polyclonal rabbit anti-gp120 Ab (Sino Biological Inc). .. The untreated gp120 had a MW≈120 kDa, whereas the EndoH-treated one had MW≈80 kDa. (B ) EndoH-untreated recombinant gp120ADA and gp120HXBc2 were cross-linked with anti-His or anti-FLAG Ab, as described in materials and methods, and subsequently subjected to native non-reducing Western blot analysis (as described by Hong PWP et al, J Virol 2002;76:12855–12865).

    Western Blot:

    Article Title: Binding of HIV-1 gp120 to DC-SIGN Promotes ASK-1-Dependent Activation-Induced Apoptosis of Human Dendritic Cells
    Article Snippet: This finding is in line with results of (of the text) and indicates that as incubation time increases, some cells at early apoptotic (AV+PI−) phase would become late apoptotic (AV+PI+) cells. are representative of 3 experiments. (TIF). .. EndoH treatment of recombinant gp120 abolished its binding to moDC. (A ) Recombinant gp120ADA supernatant was treated overnight with 25 KU of EndoH/ml as described (Hong PWP et al, J Virol 2002;76:12855–12865), and the treated and the untreated gp120ADA supernatant were subjected to Western blot assay by polyclonal rabbit anti-gp120 Ab (Sino Biological Inc). .. Figure S2 EndoH treatment of recombinant gp120 abolished its binding to moDC. (A ) Recombinant gp120ADA supernatant was treated overnight with 25 KU of EndoH/ml as described (Hong PWP et al, J Virol 2002;76:12855–12865), and the treated and the untreated gp120ADA supernatant were subjected to Western blot assay by polyclonal rabbit anti-gp120 Ab (Sino Biological Inc).

    Article Title: Binding of HIV-1 gp120 to DC-SIGN Promotes ASK-1-Dependent Activation-Induced Apoptosis of Human Dendritic Cells
    Article Snippet: EndoH treatment of recombinant gp120 abolished its binding to moDC. (A ) Recombinant gp120ADA supernatant was treated overnight with 25 KU of EndoH/ml as described (Hong PWP et al, J Virol 2002;76:12855–12865), and the treated and the untreated gp120ADA supernatant were subjected to Western blot assay by polyclonal rabbit anti-gp120 Ab (Sino Biological Inc). .. Figure S2 EndoH treatment of recombinant gp120 abolished its binding to moDC. (A ) Recombinant gp120ADA supernatant was treated overnight with 25 KU of EndoH/ml as described (Hong PWP et al, J Virol 2002;76:12855–12865), and the treated and the untreated gp120ADA supernatant were subjected to Western blot assay by polyclonal rabbit anti-gp120 Ab (Sino Biological Inc). .. The untreated gp120 had a MW≈120 kDa, whereas the EndoH-treated one had MW≈80 kDa. (B ) EndoH-untreated recombinant gp120ADA and gp120HXBc2 were cross-linked with anti-His or anti-FLAG Ab, as described in materials and methods, and subsequently subjected to native non-reducing Western blot analysis (as described by Hong PWP et al, J Virol 2002;76:12855–12865).

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  • 93
    Sino Biological gp120 antibody
    ( a ) Square wave voltammetry (SWV) results and ( b ) linear-response plot of the current peak values for the concentration of <t>gp120</t> from 0.1 pg/mL to 10 ng/mL in phosphate-buffered saline (PBS) solution. ( c ) SWV results for the concentration of gp120 from 1 pg/mL to 10 ng/mL in serum. ( d ) Selectivity test of the Ab/Cys/Au/MoS 2 /Au nanolayer on the PET substrate to various types of antigens and proteins including Hb, Mb, PSA, and Trx prepared in PBS solution.
    Gp120 Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gp120 antibody/product/Sino Biological
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gp120 antibody - by Bioz Stars, 2021-06
    93/100 stars
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    94
    Sino Biological rabbit anti gp120 polyclonal antibody
    Effects of GPI-anchored antibodies on HIV-1 Env processing. A The expression levels of gp160, <t>gp120,</t> gp41, P24, and β-actin in HEK293T cells coexpressing GPI-m36.4 or GPI-FluIgG03 and NL4-3 (left panel) or THRO.c/2626 (right panel) were detected by Western blotting. For clarity, images were spliced and grouped, and the original images are provided in the Supplementary materials . B The ratio of gp120 to gp160 in cell lysates was determined by quantifying the corresponding band intensities with ImageJ. The data shown were derived from three independent experiments, and error bars indicate standard deviations. Statistical comparisons were conducted by ANOVA (** P
    Rabbit Anti Gp120 Polyclonal Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti gp120 polyclonal antibody/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti gp120 polyclonal antibody - by Bioz Stars, 2021-06
    94/100 stars
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    93
    Sino Biological hiv 1 clade c derived recombinant gp120
    Antibody responses to <t>HIV-1</t> <t>clade</t> C gp120 and gp120-derived peptides. (A) Frequencies and intensities (y-axes: number of reactive sera; OD levels color-coded) of IgG, IgG subclass, IgA and IgM responses of African (left) and European (right) patients to recombinant gp120 (rgp120) and 24 overlapping gp120 peptides (x-axes: peptides 1–24, peptides with predicted N-linked glycosylation sites: orange). (B) Position of overlapping gp120 peptides in gp120 of the HIV-1 clade C South African strain (Ref.C.ZA) and of the reference strain HXB2 (Ref.B.FR.HXB2). Gaps (numbers of missing amino acids are displayed) in gp120 from clade C and B required for optimal sequence alignment are indicated. Relevant protein domains described for gp120 clade B are indicated (SP, signal peptide; V1-V5, variable regions 1–5). Major IgG-reactive peptides 120/15 and 120/24 are indicated in blue and red, respectively and peptides containing amino acids involved in CD4 binding are shown in yellow. (C) Surface representation of the structural model of HIV-1 clade C gp120. Major antibody-reactive gp120 peptides (120/15: blue; 120/24: red) and amino acids involved in CD4 binding (yellow) are highlighted on different views of the model.
    Hiv 1 Clade C Derived Recombinant Gp120, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiv 1 clade c derived recombinant gp120/product/Sino Biological
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hiv 1 clade c derived recombinant gp120 - by Bioz Stars, 2021-06
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    Image Search Results


    ( a ) Square wave voltammetry (SWV) results and ( b ) linear-response plot of the current peak values for the concentration of gp120 from 0.1 pg/mL to 10 ng/mL in phosphate-buffered saline (PBS) solution. ( c ) SWV results for the concentration of gp120 from 1 pg/mL to 10 ng/mL in serum. ( d ) Selectivity test of the Ab/Cys/Au/MoS 2 /Au nanolayer on the PET substrate to various types of antigens and proteins including Hb, Mb, PSA, and Trx prepared in PBS solution.

    Journal: Nanomaterials

    Article Title: Flexible HIV-1 Biosensor Based on the Au/MoS2 Nanoparticles/Au Nanolayer on the PET Substrate

    doi: 10.3390/nano9081076

    Figure Lengend Snippet: ( a ) Square wave voltammetry (SWV) results and ( b ) linear-response plot of the current peak values for the concentration of gp120 from 0.1 pg/mL to 10 ng/mL in phosphate-buffered saline (PBS) solution. ( c ) SWV results for the concentration of gp120 from 1 pg/mL to 10 ng/mL in serum. ( d ) Selectivity test of the Ab/Cys/Au/MoS 2 /Au nanolayer on the PET substrate to various types of antigens and proteins including Hb, Mb, PSA, and Trx prepared in PBS solution.

    Article Snippet: Fabrication of the Au/MoS2 /Au Nanolayer on the PET Substrate, and Immobilization of the gp120 Antibody The fabrication of a flexible biosensor composed of Ab/Cys/Au/MoS2 /Au nanolayer on PET substrate is shown in .

    Techniques: Concentration Assay, Positron Emission Tomography

    Schematic image of the fabrication of the flexible biosensor composed of gp120 antibody (Ab)/cysteamine (Cys)/Au/MoS 2 /Au nanolayer on the polyethylene terephthalate (PET) substrate for the detection of gp120.

    Journal: Nanomaterials

    Article Title: Flexible HIV-1 Biosensor Based on the Au/MoS2 Nanoparticles/Au Nanolayer on the PET Substrate

    doi: 10.3390/nano9081076

    Figure Lengend Snippet: Schematic image of the fabrication of the flexible biosensor composed of gp120 antibody (Ab)/cysteamine (Cys)/Au/MoS 2 /Au nanolayer on the polyethylene terephthalate (PET) substrate for the detection of gp120.

    Article Snippet: Fabrication of the Au/MoS2 /Au Nanolayer on the PET Substrate, and Immobilization of the gp120 Antibody The fabrication of a flexible biosensor composed of Ab/Cys/Au/MoS2 /Au nanolayer on PET substrate is shown in .

    Techniques: Positron Emission Tomography

    Effects of GPI-anchored antibodies on HIV-1 Env processing. A The expression levels of gp160, gp120, gp41, P24, and β-actin in HEK293T cells coexpressing GPI-m36.4 or GPI-FluIgG03 and NL4-3 (left panel) or THRO.c/2626 (right panel) were detected by Western blotting. For clarity, images were spliced and grouped, and the original images are provided in the Supplementary materials . B The ratio of gp120 to gp160 in cell lysates was determined by quantifying the corresponding band intensities with ImageJ. The data shown were derived from three independent experiments, and error bars indicate standard deviations. Statistical comparisons were conducted by ANOVA (** P

    Journal: Cellular and Molecular Immunology

    Article Title: Generation of HIV-resistant cells with a single-domain antibody: implications for HIV-1 gene therapy

    doi: 10.1038/s41423-020-00627-y

    Figure Lengend Snippet: Effects of GPI-anchored antibodies on HIV-1 Env processing. A The expression levels of gp160, gp120, gp41, P24, and β-actin in HEK293T cells coexpressing GPI-m36.4 or GPI-FluIgG03 and NL4-3 (left panel) or THRO.c/2626 (right panel) were detected by Western blotting. For clarity, images were spliced and grouped, and the original images are provided in the Supplementary materials . B The ratio of gp120 to gp160 in cell lysates was determined by quantifying the corresponding band intensities with ImageJ. The data shown were derived from three independent experiments, and error bars indicate standard deviations. Statistical comparisons were conducted by ANOVA (** P

    Article Snippet: The cell proteins were separated by SDS-PAGE and transferred to a nitrocellulose membrane, which was then blocked with a 5% nonfat dry milk solution in TBS-Tween 20 at room temperature for 1 h. The membrane was incubated overnight at 4 °C with a rabbit anti-gp120 polyclonal antibody (SinoBiological, Beijing, China), the human anti-gp41 monoclonal antibody 10E8, a mouse anti-P24 antibody (Abcam), or a mouse anti-β actin antibody (Sigma).

    Techniques: Expressing, Western Blot, Derivative Assay

    Antibody responses to HIV-1 clade C gp120 and gp120-derived peptides. (A) Frequencies and intensities (y-axes: number of reactive sera; OD levels color-coded) of IgG, IgG subclass, IgA and IgM responses of African (left) and European (right) patients to recombinant gp120 (rgp120) and 24 overlapping gp120 peptides (x-axes: peptides 1–24, peptides with predicted N-linked glycosylation sites: orange). (B) Position of overlapping gp120 peptides in gp120 of the HIV-1 clade C South African strain (Ref.C.ZA) and of the reference strain HXB2 (Ref.B.FR.HXB2). Gaps (numbers of missing amino acids are displayed) in gp120 from clade C and B required for optimal sequence alignment are indicated. Relevant protein domains described for gp120 clade B are indicated (SP, signal peptide; V1-V5, variable regions 1–5). Major IgG-reactive peptides 120/15 and 120/24 are indicated in blue and red, respectively and peptides containing amino acids involved in CD4 binding are shown in yellow. (C) Surface representation of the structural model of HIV-1 clade C gp120. Major antibody-reactive gp120 peptides (120/15: blue; 120/24: red) and amino acids involved in CD4 binding (yellow) are highlighted on different views of the model.

    Journal: PLoS ONE

    Article Title: Comparison of the Specificities of IgG, IgG-Subclass, IgA and IgM Reactivities in African and European HIV-Infected Individuals with an HIV-1 Clade C Proteome-Based Array

    doi: 10.1371/journal.pone.0117204

    Figure Lengend Snippet: Antibody responses to HIV-1 clade C gp120 and gp120-derived peptides. (A) Frequencies and intensities (y-axes: number of reactive sera; OD levels color-coded) of IgG, IgG subclass, IgA and IgM responses of African (left) and European (right) patients to recombinant gp120 (rgp120) and 24 overlapping gp120 peptides (x-axes: peptides 1–24, peptides with predicted N-linked glycosylation sites: orange). (B) Position of overlapping gp120 peptides in gp120 of the HIV-1 clade C South African strain (Ref.C.ZA) and of the reference strain HXB2 (Ref.B.FR.HXB2). Gaps (numbers of missing amino acids are displayed) in gp120 from clade C and B required for optimal sequence alignment are indicated. Relevant protein domains described for gp120 clade B are indicated (SP, signal peptide; V1-V5, variable regions 1–5). Major IgG-reactive peptides 120/15 and 120/24 are indicated in blue and red, respectively and peptides containing amino acids involved in CD4 binding are shown in yellow. (C) Surface representation of the structural model of HIV-1 clade C gp120. Major antibody-reactive gp120 peptides (120/15: blue; 120/24: red) and amino acids involved in CD4 binding (yellow) are highlighted on different views of the model.

    Article Snippet: HIV-1 clade C derived recombinant gp120 (rgp120) expressed in 293 cells, was purchased from Sino Biological (Beijing, People’s Republic of China) [ ].

    Techniques: Derivative Assay, Recombinant, Sequencing, Binding Assay