recombinant c jun fusion protein  (Cell Signaling Technology Inc)


Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Structured Review

    Cell Signaling Technology Inc recombinant c jun fusion protein
    Recombinant C Jun Fusion Protein, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant c jun fusion protein/product/Cell Signaling Technology Inc
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant c jun fusion protein - by Bioz Stars, 2024-07
    93/100 stars

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    recombinant c jun fusion protein  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc recombinant c jun fusion protein
    Figure 3. TGFβ regulates c-Jun in a p38-dependent manner in PC-3U cells. ( A and B ) Cell lysates derived from PC-3U cells, treated or transfected as indicated, were subjected to immunoblotting for p-Ser63-c-Jun, total c-Jun, p-p38, p-GSK-3β Ser9, total GSK-3β and HA (p38). Actin served as internal control. ( C ) PC-3U cells transiently transfected with HA-p38 wild-type or HA-p38DN were subjected to a non-radioactive in vitro kinase assay using recombinant c-Jun, and cell lysates were subsequently subjected to immunoprecipitation (IP) with HA-antibodies. The product was immunoblotted with p-Ser63-c-Jun antibodies. ( D ) Inhibitor of p38 (SB203580) were added 1 h before treatment of cells. ( E ) PC-3U cells transiently transfected with wild-type or Ser9Ala mutant GSK-3β were treated with or without TGFβ, as indicated. The cells were then lysed and subjected to immunoblotting using p-Ser63-c-Jun and GSK-3β antisera. β-tubulin served as internal control for equal loading of proteins.
    Recombinant C Jun Fusion Protein, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant c jun fusion protein/product/Cell Signaling Technology Inc
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant c jun fusion protein - by Bioz Stars, 2024-07
    93/100 stars

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    1) Product Images from "TGFβ-induced invasion of prostate cancer cells is promoted by c-Jun-dependent transcriptional activation of Snail1"

    Article Title: TGFβ-induced invasion of prostate cancer cells is promoted by c-Jun-dependent transcriptional activation of Snail1

    Journal: Cell Cycle

    doi: 10.4161/cc.29339

    Figure 3. TGFβ regulates c-Jun in a p38-dependent manner in PC-3U cells. ( A and B ) Cell lysates derived from PC-3U cells, treated or transfected as indicated, were subjected to immunoblotting for p-Ser63-c-Jun, total c-Jun, p-p38, p-GSK-3β Ser9, total GSK-3β and HA (p38). Actin served as internal control. ( C ) PC-3U cells transiently transfected with HA-p38 wild-type or HA-p38DN were subjected to a non-radioactive in vitro kinase assay using recombinant c-Jun, and cell lysates were subsequently subjected to immunoprecipitation (IP) with HA-antibodies. The product was immunoblotted with p-Ser63-c-Jun antibodies. ( D ) Inhibitor of p38 (SB203580) were added 1 h before treatment of cells. ( E ) PC-3U cells transiently transfected with wild-type or Ser9Ala mutant GSK-3β were treated with or without TGFβ, as indicated. The cells were then lysed and subjected to immunoblotting using p-Ser63-c-Jun and GSK-3β antisera. β-tubulin served as internal control for equal loading of proteins.
    Figure Legend Snippet: Figure 3. TGFβ regulates c-Jun in a p38-dependent manner in PC-3U cells. ( A and B ) Cell lysates derived from PC-3U cells, treated or transfected as indicated, were subjected to immunoblotting for p-Ser63-c-Jun, total c-Jun, p-p38, p-GSK-3β Ser9, total GSK-3β and HA (p38). Actin served as internal control. ( C ) PC-3U cells transiently transfected with HA-p38 wild-type or HA-p38DN were subjected to a non-radioactive in vitro kinase assay using recombinant c-Jun, and cell lysates were subsequently subjected to immunoprecipitation (IP) with HA-antibodies. The product was immunoblotted with p-Ser63-c-Jun antibodies. ( D ) Inhibitor of p38 (SB203580) were added 1 h before treatment of cells. ( E ) PC-3U cells transiently transfected with wild-type or Ser9Ala mutant GSK-3β were treated with or without TGFβ, as indicated. The cells were then lysed and subjected to immunoblotting using p-Ser63-c-Jun and GSK-3β antisera. β-tubulin served as internal control for equal loading of proteins.

    Techniques Used: Derivative Assay, Transfection, Western Blot, In Vitro, Kinase Assay, Recombinant, Immunoprecipitation, Mutagenesis

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  • 93
    Cell Signaling Technology Inc recombinant c jun fusion protein
    Recombinant C Jun Fusion Protein, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant c jun fusion protein/product/Cell Signaling Technology Inc
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant c jun fusion protein - by Bioz Stars, 2024-07
    93/100 stars
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