recombinant bovine il 4  (Kingfisher Biotech)


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    Structured Review

    Kingfisher Biotech recombinant bovine il 4
    IL-6 concentration in supernatants from splenic macrophages stimulated with LPS (1000 ng/ml) and IFNγ (20 ng/ml) or <t>IL-4</t> (20 ng/ml) for 20 h. OD values were determined from samples comprising combined supernatants from triplicate wells, run in duplicate. Results for two individual animals ( a and b ) are shown
    Recombinant Bovine Il 4, supplied by Kingfisher Biotech, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant bovine il 4/product/Kingfisher Biotech
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    recombinant bovine il 4 - by Bioz Stars, 2022-12
    91/100 stars

    Images

    1) Product Images from "Stimulation of bovine monocyte-derived macrophages with lipopolysaccharide, interferon-ɣ, Interleukin-4 or Interleukin-13 does not induce detectable changes in nitric oxide or arginase activity"

    Article Title: Stimulation of bovine monocyte-derived macrophages with lipopolysaccharide, interferon-ɣ, Interleukin-4 or Interleukin-13 does not induce detectable changes in nitric oxide or arginase activity

    Journal: BMC Veterinary Research

    doi: 10.1186/s12917-019-1785-0

    IL-6 concentration in supernatants from splenic macrophages stimulated with LPS (1000 ng/ml) and IFNγ (20 ng/ml) or IL-4 (20 ng/ml) for 20 h. OD values were determined from samples comprising combined supernatants from triplicate wells, run in duplicate. Results for two individual animals ( a and b ) are shown
    Figure Legend Snippet: IL-6 concentration in supernatants from splenic macrophages stimulated with LPS (1000 ng/ml) and IFNγ (20 ng/ml) or IL-4 (20 ng/ml) for 20 h. OD values were determined from samples comprising combined supernatants from triplicate wells, run in duplicate. Results for two individual animals ( a and b ) are shown

    Techniques Used: Concentration Assay

    Levels of nitric oxide (1a 1b) and chitinase (1e 1f) in monocyte-derived and splenic macrophage cell supernatants and arginase (1c 1d) in cell lysates following stimulation with LPS and/or cytokines (LPS 1000 ng/ml, IFNɣ 20 ng/ml, IL-4 20 ng/ml and IL-13 20 ng/ml) for 40 h. Levels of enzyme product were determined by measuring OD values and comparison with standard curves. Means and standard errors of data from triplicate cultures from representative individual animals are shown, monocyte-derived macrophages all being obtained from one animal and splenic macrophages all from another individual. Similar findings were recorded from all individuals tested ( n = 5 MDM, n = 7 SM) for stimulation times of 12–72 h
    Figure Legend Snippet: Levels of nitric oxide (1a 1b) and chitinase (1e 1f) in monocyte-derived and splenic macrophage cell supernatants and arginase (1c 1d) in cell lysates following stimulation with LPS and/or cytokines (LPS 1000 ng/ml, IFNɣ 20 ng/ml, IL-4 20 ng/ml and IL-13 20 ng/ml) for 40 h. Levels of enzyme product were determined by measuring OD values and comparison with standard curves. Means and standard errors of data from triplicate cultures from representative individual animals are shown, monocyte-derived macrophages all being obtained from one animal and splenic macrophages all from another individual. Similar findings were recorded from all individuals tested ( n = 5 MDM, n = 7 SM) for stimulation times of 12–72 h

    Techniques Used: Derivative Assay

    2) Product Images from "Stimulation of bovine monocyte-derived macrophages with lipopolysaccharide, interferon-ɣ, Interleukin-4 or Interleukin-13 does not induce detectable changes in nitric oxide or arginase activity"

    Article Title: Stimulation of bovine monocyte-derived macrophages with lipopolysaccharide, interferon-ɣ, Interleukin-4 or Interleukin-13 does not induce detectable changes in nitric oxide or arginase activity

    Journal: BMC Veterinary Research

    doi: 10.1186/s12917-019-1785-0

    IL-6 concentration in supernatants from splenic macrophages stimulated with LPS (1000 ng/ml) and IFNγ (20 ng/ml) or IL-4 (20 ng/ml) for 20 h. OD values were determined from samples comprising combined supernatants from triplicate wells, run in duplicate. Results for two individual animals ( a and b ) are shown
    Figure Legend Snippet: IL-6 concentration in supernatants from splenic macrophages stimulated with LPS (1000 ng/ml) and IFNγ (20 ng/ml) or IL-4 (20 ng/ml) for 20 h. OD values were determined from samples comprising combined supernatants from triplicate wells, run in duplicate. Results for two individual animals ( a and b ) are shown

    Techniques Used: Concentration Assay

    Fold changes in MDM production of nos2 ( a ) and arg1 ( b ) following stimulation with LPS (1000 ng/ml) IFNγ (20 ng/ml) or IL-4 (20 ng/ml) IL-13 (20 ng/ml) compared with changes in control cells, normalised with reference to transcription levels of GAPDH at 12 24 h. Fold changes in levels of nitric oxide in MDM cell supernatants from stimulated cells in comparison to unstimulated controls are shown in c . Supernatants and mRNA were harvested contemporaneously from the same cell populations and were performed in triplicate. Data from the same representative individual are shown in a , b and c
    Figure Legend Snippet: Fold changes in MDM production of nos2 ( a ) and arg1 ( b ) following stimulation with LPS (1000 ng/ml) IFNγ (20 ng/ml) or IL-4 (20 ng/ml) IL-13 (20 ng/ml) compared with changes in control cells, normalised with reference to transcription levels of GAPDH at 12 24 h. Fold changes in levels of nitric oxide in MDM cell supernatants from stimulated cells in comparison to unstimulated controls are shown in c . Supernatants and mRNA were harvested contemporaneously from the same cell populations and were performed in triplicate. Data from the same representative individual are shown in a , b and c

    Techniques Used:

    Levels of nitric oxide (1a 1b) and chitinase (1e 1f) in monocyte-derived and splenic macrophage cell supernatants and arginase (1c 1d) in cell lysates following stimulation with LPS and/or cytokines (LPS 1000 ng/ml, IFNɣ 20 ng/ml, IL-4 20 ng/ml and IL-13 20 ng/ml) for 40 h. Levels of enzyme product were determined by measuring OD values and comparison with standard curves. Means and standard errors of data from triplicate cultures from representative individual animals are shown, monocyte-derived macrophages all being obtained from one animal and splenic macrophages all from another individual. Similar findings were recorded from all individuals tested ( n = 5 MDM, n = 7 SM) for stimulation times of 12–72 h
    Figure Legend Snippet: Levels of nitric oxide (1a 1b) and chitinase (1e 1f) in monocyte-derived and splenic macrophage cell supernatants and arginase (1c 1d) in cell lysates following stimulation with LPS and/or cytokines (LPS 1000 ng/ml, IFNɣ 20 ng/ml, IL-4 20 ng/ml and IL-13 20 ng/ml) for 40 h. Levels of enzyme product were determined by measuring OD values and comparison with standard curves. Means and standard errors of data from triplicate cultures from representative individual animals are shown, monocyte-derived macrophages all being obtained from one animal and splenic macrophages all from another individual. Similar findings were recorded from all individuals tested ( n = 5 MDM, n = 7 SM) for stimulation times of 12–72 h

    Techniques Used: Derivative Assay

    3) Product Images from "Stimulation of bovine monocyte-derived macrophages with lipopolysaccharide, interferon-ɣ, Interleukin-4 or Interleukin-13 does not induce detectable changes in nitric oxide or arginase activity"

    Article Title: Stimulation of bovine monocyte-derived macrophages with lipopolysaccharide, interferon-ɣ, Interleukin-4 or Interleukin-13 does not induce detectable changes in nitric oxide or arginase activity

    Journal: BMC Veterinary Research

    doi: 10.1186/s12917-019-1785-0

    IL-6 concentration in supernatants from splenic macrophages stimulated with LPS (1000 ng/ml) and IFNγ (20 ng/ml) or IL-4 (20 ng/ml) for 20 h. OD values were determined from samples comprising combined supernatants from triplicate wells, run in duplicate. Results for two individual animals ( a and b ) are shown
    Figure Legend Snippet: IL-6 concentration in supernatants from splenic macrophages stimulated with LPS (1000 ng/ml) and IFNγ (20 ng/ml) or IL-4 (20 ng/ml) for 20 h. OD values were determined from samples comprising combined supernatants from triplicate wells, run in duplicate. Results for two individual animals ( a and b ) are shown

    Techniques Used: Concentration Assay

    Fold changes in MDM production of nos2 ( a ) and arg1 ( b ) following stimulation with LPS (1000 ng/ml) IFNγ (20 ng/ml) or IL-4 (20 ng/ml) IL-13 (20 ng/ml) compared with changes in control cells, normalised with reference to transcription levels of GAPDH at 12 24 h. Fold changes in levels of nitric oxide in MDM cell supernatants from stimulated cells in comparison to unstimulated controls are shown in c . Supernatants and mRNA were harvested contemporaneously from the same cell populations and were performed in triplicate. Data from the same representative individual are shown in a , b and c
    Figure Legend Snippet: Fold changes in MDM production of nos2 ( a ) and arg1 ( b ) following stimulation with LPS (1000 ng/ml) IFNγ (20 ng/ml) or IL-4 (20 ng/ml) IL-13 (20 ng/ml) compared with changes in control cells, normalised with reference to transcription levels of GAPDH at 12 24 h. Fold changes in levels of nitric oxide in MDM cell supernatants from stimulated cells in comparison to unstimulated controls are shown in c . Supernatants and mRNA were harvested contemporaneously from the same cell populations and were performed in triplicate. Data from the same representative individual are shown in a , b and c

    Techniques Used:

    Levels of nitric oxide (1a 1b) and chitinase (1e 1f) in monocyte-derived and splenic macrophage cell supernatants and arginase (1c 1d) in cell lysates following stimulation with LPS and/or cytokines (LPS 1000 ng/ml, IFNɣ 20 ng/ml, IL-4 20 ng/ml and IL-13 20 ng/ml) for 40 h. Levels of enzyme product were determined by measuring OD values and comparison with standard curves. Means and standard errors of data from triplicate cultures from representative individual animals are shown, monocyte-derived macrophages all being obtained from one animal and splenic macrophages all from another individual. Similar findings were recorded from all individuals tested ( n = 5 MDM, n = 7 SM) for stimulation times of 12–72 h
    Figure Legend Snippet: Levels of nitric oxide (1a 1b) and chitinase (1e 1f) in monocyte-derived and splenic macrophage cell supernatants and arginase (1c 1d) in cell lysates following stimulation with LPS and/or cytokines (LPS 1000 ng/ml, IFNɣ 20 ng/ml, IL-4 20 ng/ml and IL-13 20 ng/ml) for 40 h. Levels of enzyme product were determined by measuring OD values and comparison with standard curves. Means and standard errors of data from triplicate cultures from representative individual animals are shown, monocyte-derived macrophages all being obtained from one animal and splenic macrophages all from another individual. Similar findings were recorded from all individuals tested ( n = 5 MDM, n = 7 SM) for stimulation times of 12–72 h

    Techniques Used: Derivative Assay

    4) Product Images from "Bovine WC1+ and WC1neg γδ T Lymphocytes Influence Monocyte Differentiation and Monocyte-Derived Dendritic Cell Maturation during In Vitro Mycobacterium avium Subspecies paratuberculosis Infection"

    Article Title: Bovine WC1+ and WC1neg γδ T Lymphocytes Influence Monocyte Differentiation and Monocyte-Derived Dendritic Cell Maturation during In Vitro Mycobacterium avium Subspecies paratuberculosis Infection

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2017.00534

    Dendritic cell maturation assays . Immature monocyte-derived dendritic cells (iMDDCs) were generated by adding IL-4 and GM-CSF to fresh monocyte cultures. After 6 days of differentiation, 1 × 10 6 sorted γδ T lymphocytes were added to wells containing 2 × 10 5 iMDDCs for 48 h (iMDDC + WC1 + and iMDDC + WC1 neg ). On day 6, live Mycobacterium avium subspecies paratuberculosis was added at a multiplicity of infection of 10:1 to evaluate if it affected iMDDC maturation after 48 h.
    Figure Legend Snippet: Dendritic cell maturation assays . Immature monocyte-derived dendritic cells (iMDDCs) were generated by adding IL-4 and GM-CSF to fresh monocyte cultures. After 6 days of differentiation, 1 × 10 6 sorted γδ T lymphocytes were added to wells containing 2 × 10 5 iMDDCs for 48 h (iMDDC + WC1 + and iMDDC + WC1 neg ). On day 6, live Mycobacterium avium subspecies paratuberculosis was added at a multiplicity of infection of 10:1 to evaluate if it affected iMDDC maturation after 48 h.

    Techniques Used: Derivative Assay, Generated, Infection

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    Kingfisher Biotech recombinant bovine il 4
    IL-6 concentration in supernatants from splenic macrophages stimulated with LPS (1000 ng/ml) and IFNγ (20 ng/ml) or <t>IL-4</t> (20 ng/ml) for 20 h. OD values were determined from samples comprising combined supernatants from triplicate wells, run in duplicate. Results for two individual animals ( a and b ) are shown
    Recombinant Bovine Il 4, supplied by Kingfisher Biotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant bovine il 4/product/Kingfisher Biotech
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant bovine il 4 - by Bioz Stars, 2022-12
    94/100 stars
      Buy from Supplier

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    IL-6 concentration in supernatants from splenic macrophages stimulated with LPS (1000 ng/ml) and IFNγ (20 ng/ml) or IL-4 (20 ng/ml) for 20 h. OD values were determined from samples comprising combined supernatants from triplicate wells, run in duplicate. Results for two individual animals ( a and b ) are shown

    Journal: BMC Veterinary Research

    Article Title: Stimulation of bovine monocyte-derived macrophages with lipopolysaccharide, interferon-ɣ, Interleukin-4 or Interleukin-13 does not induce detectable changes in nitric oxide or arginase activity

    doi: 10.1186/s12917-019-1785-0

    Figure Lengend Snippet: IL-6 concentration in supernatants from splenic macrophages stimulated with LPS (1000 ng/ml) and IFNγ (20 ng/ml) or IL-4 (20 ng/ml) for 20 h. OD values were determined from samples comprising combined supernatants from triplicate wells, run in duplicate. Results for two individual animals ( a and b ) are shown

    Article Snippet: MDM or SM were stimulated by addition of 50–2000 ng/ml LPS from E. coli 0111:B4 (Insight Biotechnology Ltd., Middlesex) and/or 20-100 ng/ml bovine IFNγ (ThermoFisher Scientific, UK) or with 10-40 ng/ml recombinant bovine IL-4 (Kingfisher Biotech Inc., St Paul) and/or 10-20 ng/ml recombinant bovine IL-13 (Kingfisher Biotech Inc).

    Techniques: Concentration Assay

    Levels of nitric oxide (1a 1b) and chitinase (1e 1f) in monocyte-derived and splenic macrophage cell supernatants and arginase (1c 1d) in cell lysates following stimulation with LPS and/or cytokines (LPS 1000 ng/ml, IFNɣ 20 ng/ml, IL-4 20 ng/ml and IL-13 20 ng/ml) for 40 h. Levels of enzyme product were determined by measuring OD values and comparison with standard curves. Means and standard errors of data from triplicate cultures from representative individual animals are shown, monocyte-derived macrophages all being obtained from one animal and splenic macrophages all from another individual. Similar findings were recorded from all individuals tested ( n = 5 MDM, n = 7 SM) for stimulation times of 12–72 h

    Journal: BMC Veterinary Research

    Article Title: Stimulation of bovine monocyte-derived macrophages with lipopolysaccharide, interferon-ɣ, Interleukin-4 or Interleukin-13 does not induce detectable changes in nitric oxide or arginase activity

    doi: 10.1186/s12917-019-1785-0

    Figure Lengend Snippet: Levels of nitric oxide (1a 1b) and chitinase (1e 1f) in monocyte-derived and splenic macrophage cell supernatants and arginase (1c 1d) in cell lysates following stimulation with LPS and/or cytokines (LPS 1000 ng/ml, IFNɣ 20 ng/ml, IL-4 20 ng/ml and IL-13 20 ng/ml) for 40 h. Levels of enzyme product were determined by measuring OD values and comparison with standard curves. Means and standard errors of data from triplicate cultures from representative individual animals are shown, monocyte-derived macrophages all being obtained from one animal and splenic macrophages all from another individual. Similar findings were recorded from all individuals tested ( n = 5 MDM, n = 7 SM) for stimulation times of 12–72 h

    Article Snippet: MDM or SM were stimulated by addition of 50–2000 ng/ml LPS from E. coli 0111:B4 (Insight Biotechnology Ltd., Middlesex) and/or 20-100 ng/ml bovine IFNγ (ThermoFisher Scientific, UK) or with 10-40 ng/ml recombinant bovine IL-4 (Kingfisher Biotech Inc., St Paul) and/or 10-20 ng/ml recombinant bovine IL-13 (Kingfisher Biotech Inc).

    Techniques: Derivative Assay

    IL-6 concentration in supernatants from splenic macrophages stimulated with LPS (1000 ng/ml) and IFNγ (20 ng/ml) or IL-4 (20 ng/ml) for 20 h. OD values were determined from samples comprising combined supernatants from triplicate wells, run in duplicate. Results for two individual animals ( a and b ) are shown

    Journal: BMC Veterinary Research

    Article Title: Stimulation of bovine monocyte-derived macrophages with lipopolysaccharide, interferon-ɣ, Interleukin-4 or Interleukin-13 does not induce detectable changes in nitric oxide or arginase activity

    doi: 10.1186/s12917-019-1785-0

    Figure Lengend Snippet: IL-6 concentration in supernatants from splenic macrophages stimulated with LPS (1000 ng/ml) and IFNγ (20 ng/ml) or IL-4 (20 ng/ml) for 20 h. OD values were determined from samples comprising combined supernatants from triplicate wells, run in duplicate. Results for two individual animals ( a and b ) are shown

    Article Snippet: Cell stimulation MDM or SM were stimulated by addition of 50–2000 ng/ml LPS from E. coli 0111:B4 (Insight Biotechnology Ltd., Middlesex) and/or 20-100 ng/ml bovine IFNγ (ThermoFisher Scientific, UK) or with 10-40 ng/ml recombinant bovine IL-4 (Kingfisher Biotech Inc., St Paul) and/or 10-20 ng/ml recombinant bovine IL-13 (Kingfisher Biotech Inc).

    Techniques: Concentration Assay

    Fold changes in MDM production of nos2 ( a ) and arg1 ( b ) following stimulation with LPS (1000 ng/ml) IFNγ (20 ng/ml) or IL-4 (20 ng/ml) IL-13 (20 ng/ml) compared with changes in control cells, normalised with reference to transcription levels of GAPDH at 12 24 h. Fold changes in levels of nitric oxide in MDM cell supernatants from stimulated cells in comparison to unstimulated controls are shown in c . Supernatants and mRNA were harvested contemporaneously from the same cell populations and were performed in triplicate. Data from the same representative individual are shown in a , b and c

    Journal: BMC Veterinary Research

    Article Title: Stimulation of bovine monocyte-derived macrophages with lipopolysaccharide, interferon-ɣ, Interleukin-4 or Interleukin-13 does not induce detectable changes in nitric oxide or arginase activity

    doi: 10.1186/s12917-019-1785-0

    Figure Lengend Snippet: Fold changes in MDM production of nos2 ( a ) and arg1 ( b ) following stimulation with LPS (1000 ng/ml) IFNγ (20 ng/ml) or IL-4 (20 ng/ml) IL-13 (20 ng/ml) compared with changes in control cells, normalised with reference to transcription levels of GAPDH at 12 24 h. Fold changes in levels of nitric oxide in MDM cell supernatants from stimulated cells in comparison to unstimulated controls are shown in c . Supernatants and mRNA were harvested contemporaneously from the same cell populations and were performed in triplicate. Data from the same representative individual are shown in a , b and c

    Article Snippet: Cell stimulation MDM or SM were stimulated by addition of 50–2000 ng/ml LPS from E. coli 0111:B4 (Insight Biotechnology Ltd., Middlesex) and/or 20-100 ng/ml bovine IFNγ (ThermoFisher Scientific, UK) or with 10-40 ng/ml recombinant bovine IL-4 (Kingfisher Biotech Inc., St Paul) and/or 10-20 ng/ml recombinant bovine IL-13 (Kingfisher Biotech Inc).

    Techniques:

    Levels of nitric oxide (1a 1b) and chitinase (1e 1f) in monocyte-derived and splenic macrophage cell supernatants and arginase (1c 1d) in cell lysates following stimulation with LPS and/or cytokines (LPS 1000 ng/ml, IFNɣ 20 ng/ml, IL-4 20 ng/ml and IL-13 20 ng/ml) for 40 h. Levels of enzyme product were determined by measuring OD values and comparison with standard curves. Means and standard errors of data from triplicate cultures from representative individual animals are shown, monocyte-derived macrophages all being obtained from one animal and splenic macrophages all from another individual. Similar findings were recorded from all individuals tested ( n = 5 MDM, n = 7 SM) for stimulation times of 12–72 h

    Journal: BMC Veterinary Research

    Article Title: Stimulation of bovine monocyte-derived macrophages with lipopolysaccharide, interferon-ɣ, Interleukin-4 or Interleukin-13 does not induce detectable changes in nitric oxide or arginase activity

    doi: 10.1186/s12917-019-1785-0

    Figure Lengend Snippet: Levels of nitric oxide (1a 1b) and chitinase (1e 1f) in monocyte-derived and splenic macrophage cell supernatants and arginase (1c 1d) in cell lysates following stimulation with LPS and/or cytokines (LPS 1000 ng/ml, IFNɣ 20 ng/ml, IL-4 20 ng/ml and IL-13 20 ng/ml) for 40 h. Levels of enzyme product were determined by measuring OD values and comparison with standard curves. Means and standard errors of data from triplicate cultures from representative individual animals are shown, monocyte-derived macrophages all being obtained from one animal and splenic macrophages all from another individual. Similar findings were recorded from all individuals tested ( n = 5 MDM, n = 7 SM) for stimulation times of 12–72 h

    Article Snippet: Cell stimulation MDM or SM were stimulated by addition of 50–2000 ng/ml LPS from E. coli 0111:B4 (Insight Biotechnology Ltd., Middlesex) and/or 20-100 ng/ml bovine IFNγ (ThermoFisher Scientific, UK) or with 10-40 ng/ml recombinant bovine IL-4 (Kingfisher Biotech Inc., St Paul) and/or 10-20 ng/ml recombinant bovine IL-13 (Kingfisher Biotech Inc).

    Techniques: Derivative Assay