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Eiken Chemical real time turbidimeter
Real Time Turbidimeter, supplied by Eiken Chemical, used in various techniques. Bioz Stars score: 89/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/real time turbidimeter/product/Eiken Chemical
Average 89 stars, based on 9 article reviews
Price from $9.99 to $1999.99
real time turbidimeter - by Bioz Stars, 2020-08
89/100 stars

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Article Title: LAMP real-time turbidity detection for fowl adenovirus
Article Snippet: .. White precipitates of Mg2 P2 O7 were produced during the LAMP reaction, and thus, the turbidity of the reaction tube was monitored every 6 s by a real-time turbidimeter (La-320, Eiken Chemical Co., Japan). ..

Concentration Assay:

Article Title: Establishment and application of isothermal amplification techniques for the detection of heat-stable I enterotoxin of enterotoxigenic Escherichia coli
Article Snippet: .. The concentration of Mg2+ was varied from 1.0 mM to 4.0 mM, that of Bst -DNA polymerase from 8 U to 20 U, dNTPs from 1.0 mM—3.0 mM and the concentration of betaine from 0.6 M to 1.4 M. The last parameter optimized was duration of incubation which ranged from 30 min to 120 min. All optimized assays were tested in triplicate and the results recorded using a real-time turbidimeter (Eiken Chemical Co., Ltd, Tokyo, Japan). ..

Incubation:

Article Title: Establishment and application of isothermal amplification techniques for the detection of heat-stable I enterotoxin of enterotoxigenic Escherichia coli
Article Snippet: .. The concentration of Mg2+ was varied from 1.0 mM to 4.0 mM, that of Bst -DNA polymerase from 8 U to 20 U, dNTPs from 1.0 mM—3.0 mM and the concentration of betaine from 0.6 M to 1.4 M. The last parameter optimized was duration of incubation which ranged from 30 min to 120 min. All optimized assays were tested in triplicate and the results recorded using a real-time turbidimeter (Eiken Chemical Co., Ltd, Tokyo, Japan). ..

Article Title: Loop-Mediated Isothermal Amplification for Rickettsia typhi (the Causal Agent of Murine Typhus): Problems with Diagnosis at the Limit of Detection
Article Snippet: .. The reaction mix was incubated in a real-time turbidimeter (model LA-320CE; Eiken Chemical Co., Ltd., Tokyo, Japan) at 60°C for 90 min, followed by enzyme inactivation (80°C for 5 min). .. Positive and nontemplate controls were included in each run and reliably produced the correct results.

Amplification:

Article Title: Rapid Detection of Candida albicans by Polymerase Spiral Reaction Assay in Clinical Blood Samples
Article Snippet: .. Amplification was monitored by two methods, turbidity monitoring with a real-time turbidimeter (LA-320c; Eiken Chemical, Japan) and direct visual in the presence of pH dye. ..

Article Title: Survey and rapid detection of Klebsiella pneumoniae in clinical samples targeting the rcsA gene in Beijing, China
Article Snippet: .. LAMP reaction The LAMP reactions were performed in a final volume of 25 μl containing 12.5 μl reaction mixture, 1 μl Bst DNA polymerase, 2 μl template by using the Loopamp DNA Amplification kit (Loopamp DNA Amplification Kit; Eiken Chemical Co., Ltd, Tochigi, Japan) for real-time turbidimeter and another 1 μl calcein/Mn2+ solution (Eiken Chemical Co., Ltd) for visual detection. .. Primers were used at a concentration of 40 pmol for FIP and BIP, 20 pmol for LB and LF, and 5 pmol for F3 and B3.

Lamp Assay:

Article Title: The development of loop-mediated isothermal amplification targeting alpha-tubulin DNA for the rapid detection of Plasmodium vivax
Article Snippet: .. Results from the LAMP assay were independently recorded using the UV lamp in real-time turbidimeter (Eiken Chemical Co., Ltd.) by two investigators who were blinded to the identity of the samples (Table and Table ). .. After scoring the results of fluorescence detection, all the LAMP products were electrophoresed on a 2% agarose gels for detection of LAMP amplification.

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    Eiken Chemical loopamp real time turbidimeter
    Relationship of turbidity with copy number of the 2009 H1N1 influenza A virus. Real‐time turbidity was detected with a <t>Loopamp</t> real‐time turbidimeter. The time required to reach the threshold turbidity level (0.1) (threshold time) were plotted against the copy numbers of the samples determined by real‐time RT‐PCR.
    Loopamp Real Time Turbidimeter, supplied by Eiken Chemical, used in various techniques. Bioz Stars score: 91/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/loopamp real time turbidimeter/product/Eiken Chemical
    Average 91 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    loopamp real time turbidimeter - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    85
    Eiken Chemical loopamp real time la 320c turbidimeter
    Sensitivity of the hMPV genotype-specific RT-LAMP assay. Serial diluted RNAs ranging from 10 −6 to 10 −13 were used to determine the detection limits of the hMPV genotype-specific RT-LAMP. The results of the genotype-specific RT-LAMP were assessed by the <t>Loopamp</t> ® real-time <t>LA-320C</t> turbidimeter.
    Loopamp Real Time La 320c Turbidimeter, supplied by Eiken Chemical, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/loopamp real time la 320c turbidimeter/product/Eiken Chemical
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    loopamp real time la 320c turbidimeter - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

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    Relationship of turbidity with copy number of the 2009 H1N1 influenza A virus. Real‐time turbidity was detected with a Loopamp real‐time turbidimeter. The time required to reach the threshold turbidity level (0.1) (threshold time) were plotted against the copy numbers of the samples determined by real‐time RT‐PCR.

    Journal: Journal of Medical Virology

    Article Title: Mobile and accurate detection system for infection by the 2009 pandemic influenza A (H1N1) virus with a pocket‐warmer reverse‐transcriptase loop‐mediated isothermal amplification

    doi: 10.1002/jmv.22031

    Figure Lengend Snippet: Relationship of turbidity with copy number of the 2009 H1N1 influenza A virus. Real‐time turbidity was detected with a Loopamp real‐time turbidimeter. The time required to reach the threshold turbidity level (0.1) (threshold time) were plotted against the copy numbers of the samples determined by real‐time RT‐PCR.

    Article Snippet: For the conventional RT‐LAMP method, 50 µl of RT‐LAMP mixture including 9 µl of RNA sample was incubated in a Loopamp Real‐Time Turbidimeter (LA‐320C; Eiken Chemical) for 60 min at 60°C and then for 5 min at 80°C to terminate the reaction.

    Techniques: Quantitative RT-PCR

    Detection limit of Azumiobodo hoyamushi 18S rDNA LAMP assays (A). LAMP assays were performed using serial dilutions of A. hoyamushi genomic DNA (1 ng to 1 fg per reaction). Distilled water was used as a negative control. LAMP products were visualized by gel electrophoresis (B) and using Loopamp® fluorescent detection reagent (FD) (C). (B, C) Lane M, 100-bp DNA marker; lane 1, 1 ng; lane 2, 100 pg; lane 3, 10 pg; lane 4, 1 pg; lane 5, 100 fg; lane 6, 10 fg; lane 7, 1 fg of A. hoyamushi genomic DNA; lane 8, distilled water; and lane 9, LAMP product after Mbo I digestion. (D-E) A. hoyamushi at a density of 1×10 3 parasites/µl was serially diluted and tested (D) using the LAMP assay (D) and by PCR (E) using F3 and B3 primers. Lane M, 100-bp DNA marker; lane 1, 1,000; lane 2, 100; lane 3, 10; lane 4, 1; lane 5, 0.1; lane 6, 0.01 of parasites per reaction; lane 7, distilled water. A. hoyamushi genomic DNA was prepared using DNeasy tissue kits (Qiagen) from in vitro cultured A. hoyamushi species [ 9 ] which were kindly provided by Dr. Kyung Il Park (Kunsan National University, Gunsan, Korea).

    Journal: The Korean Journal of Parasitology

    Article Title: Development of Loop-Mediated Isothermal Amplification Targeting 18S Ribosomal DNA for Rapid Detection of Azumiobodo hoyamushi (Kinetoplastea)

    doi: 10.3347/kjp.2014.52.3.305

    Figure Lengend Snippet: Detection limit of Azumiobodo hoyamushi 18S rDNA LAMP assays (A). LAMP assays were performed using serial dilutions of A. hoyamushi genomic DNA (1 ng to 1 fg per reaction). Distilled water was used as a negative control. LAMP products were visualized by gel electrophoresis (B) and using Loopamp® fluorescent detection reagent (FD) (C). (B, C) Lane M, 100-bp DNA marker; lane 1, 1 ng; lane 2, 100 pg; lane 3, 10 pg; lane 4, 1 pg; lane 5, 100 fg; lane 6, 10 fg; lane 7, 1 fg of A. hoyamushi genomic DNA; lane 8, distilled water; and lane 9, LAMP product after Mbo I digestion. (D-E) A. hoyamushi at a density of 1×10 3 parasites/µl was serially diluted and tested (D) using the LAMP assay (D) and by PCR (E) using F3 and B3 primers. Lane M, 100-bp DNA marker; lane 1, 1,000; lane 2, 100; lane 3, 10; lane 4, 1; lane 5, 0.1; lane 6, 0.01 of parasites per reaction; lane 7, distilled water. A. hoyamushi genomic DNA was prepared using DNeasy tissue kits (Qiagen) from in vitro cultured A. hoyamushi species [ 9 ] which were kindly provided by Dr. Kyung Il Park (Kunsan National University, Gunsan, Korea).

    Article Snippet: A. hoyamushi LAMP was performed for 90 min at 64℃ in a 25 µl mixture containing 40 pmol each of FIP and BIP, 5 pmol each of F3 and B3, 20 pmol each of LF and LB, 1.4 mM of deoxynucleoside triphosphates, 0.8 M betaine, and 1 µl of Bst DNA polymerase (NEB, Beverly, Massachusetts, USA) in 2.5 µl of buffer [20 mM Tris-HCl pH 8.8, 10 mM KCl, 10 mM (NH4 )2 SO4 , 8 mM MgSO4 , and 0.1% Tween 20], and 1 µl of template DNA in a Loopamp real-time turbidimeter (Realoop-30; Eiken Chemical Co., Tokyo, Japan).

    Techniques: Negative Control, Nucleic Acid Electrophoresis, Marker, Lamp Assay, Polymerase Chain Reaction, In Vitro, Cell Culture

    Differentiation of Akebiae Caulis from Aristolochiae Manshuriensis Caulis using LAMP. Gmt-1 to Gmt-3, Aristolochia manshuriensis ; Mt-1 to Mt-3, Akebia trifoliata ; Mt-4 to Mt-6, Ak. trifoliata var. australis ; Mt-7 to Mt-9, Ak. quinata ; Neg, negative control (double-distilled water). (A) Turbidity was monitored by a Loopamp real-time turbidimeter at 400 nm every 6 s, amplification was performed at 65°C for 60 min; (B) A visual color change detection method was compared. 1 μl of calcein (fluorescent detection reagent) was added to 25 μl of LAMP reaction mixture before the LAMP reaction.

    Journal: Frontiers in Plant Science

    Article Title: Rapid Identification of Officinal Akebiae Caulis and Its Toxic Adulterant Aristolochiae Manshuriensis Caulis (Aristolochia manshuriensis) by Loop-Mediated Isothermal Amplification

    doi: 10.3389/fpls.2016.00887

    Figure Lengend Snippet: Differentiation of Akebiae Caulis from Aristolochiae Manshuriensis Caulis using LAMP. Gmt-1 to Gmt-3, Aristolochia manshuriensis ; Mt-1 to Mt-3, Akebia trifoliata ; Mt-4 to Mt-6, Ak. trifoliata var. australis ; Mt-7 to Mt-9, Ak. quinata ; Neg, negative control (double-distilled water). (A) Turbidity was monitored by a Loopamp real-time turbidimeter at 400 nm every 6 s, amplification was performed at 65°C for 60 min; (B) A visual color change detection method was compared. 1 μl of calcein (fluorescent detection reagent) was added to 25 μl of LAMP reaction mixture before the LAMP reaction.

    Article Snippet: First, we monitored real-time amplification of LAMP assays using a Loopamp real-time turbidimeter (LA-230; Eiken Chemical Co., Ltd., Tochigi, Japan) to monitor turbidity by recording the optical density at 400 nm every 6 s. Second, we employed visual inspection where we observed color changes in the LAMP reaction mixtures, caused by the addition of 1 μl of calcein (Fluorescence Detection Reagent; Loopamp, Eiken China Co., Ltd., Shanghai, China) before the LAMP reaction.

    Techniques: Negative Control, Amplification

    Comparison of sensitivity between the LAMP reaction and conventional PCR for detection of Aristolochiae Manshuriensis Caulis. The pure genomic DNA extracted from Aristolochia manshuriensis was diluted in a serial 10-fold dilution. Both LAMP reaction (A) and (B) and conventional PCR (C) were carried out in duplicate for each dilution point. Tubes and lanes: 1, 42.2 ng/μl; 2, 4.22 ng/μl; 3, 422 pg/μl; 4, 42.2 pg/μl; 5, 4.22 pg/μl; 6, 422 fg/μl; 7, 42.2 fg/μl; 8, Neg, negative control (double-distilled water). (A) Turbidity was monitored by a Loopamp real-time turbidimeter at 400 nm every 6 s; (B) A visual color change detection method was compared. 1μl of calcein (fluorescent detection reagent) was added to 25 μl of LAMP reaction mixture before the LAMP reaction; (C) The PCR products were detected by 1% agarose gel electrophoresis.

    Journal: Frontiers in Plant Science

    Article Title: Rapid Identification of Officinal Akebiae Caulis and Its Toxic Adulterant Aristolochiae Manshuriensis Caulis (Aristolochia manshuriensis) by Loop-Mediated Isothermal Amplification

    doi: 10.3389/fpls.2016.00887

    Figure Lengend Snippet: Comparison of sensitivity between the LAMP reaction and conventional PCR for detection of Aristolochiae Manshuriensis Caulis. The pure genomic DNA extracted from Aristolochia manshuriensis was diluted in a serial 10-fold dilution. Both LAMP reaction (A) and (B) and conventional PCR (C) were carried out in duplicate for each dilution point. Tubes and lanes: 1, 42.2 ng/μl; 2, 4.22 ng/μl; 3, 422 pg/μl; 4, 42.2 pg/μl; 5, 4.22 pg/μl; 6, 422 fg/μl; 7, 42.2 fg/μl; 8, Neg, negative control (double-distilled water). (A) Turbidity was monitored by a Loopamp real-time turbidimeter at 400 nm every 6 s; (B) A visual color change detection method was compared. 1μl of calcein (fluorescent detection reagent) was added to 25 μl of LAMP reaction mixture before the LAMP reaction; (C) The PCR products were detected by 1% agarose gel electrophoresis.

    Article Snippet: First, we monitored real-time amplification of LAMP assays using a Loopamp real-time turbidimeter (LA-230; Eiken Chemical Co., Ltd., Tochigi, Japan) to monitor turbidity by recording the optical density at 400 nm every 6 s. Second, we employed visual inspection where we observed color changes in the LAMP reaction mixtures, caused by the addition of 1 μl of calcein (Fluorescence Detection Reagent; Loopamp, Eiken China Co., Ltd., Shanghai, China) before the LAMP reaction.

    Techniques: Polymerase Chain Reaction, Negative Control, Agarose Gel Electrophoresis

    Sensitivity of the hMPV genotype-specific RT-LAMP assay. Serial diluted RNAs ranging from 10 −6 to 10 −13 were used to determine the detection limits of the hMPV genotype-specific RT-LAMP. The results of the genotype-specific RT-LAMP were assessed by the Loopamp ® real-time LA-320C turbidimeter.

    Journal: Journal of Virological Methods

    Article Title: Identification of human metapneumovirus genotypes A and B from clinical specimens by reverse transcription loop-mediated isothermal amplification

    doi: 10.1016/j.jviromet.2013.10.037

    Figure Lengend Snippet: Sensitivity of the hMPV genotype-specific RT-LAMP assay. Serial diluted RNAs ranging from 10 −6 to 10 −13 were used to determine the detection limits of the hMPV genotype-specific RT-LAMP. The results of the genotype-specific RT-LAMP were assessed by the Loopamp ® real-time LA-320C turbidimeter.

    Article Snippet: A Loopamp® real-time LA-320C turbidimeter (Eiken Chemical, Tokyo, Japan) was used to monitor the accumulation of magnesium pyrophosphate spectrophotometrically at 650 nm.

    Techniques: RT Lamp Assay