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Eiken Chemical real time turbidimeter la 320c
Real Time Turbidimeter La 320c, supplied by Eiken Chemical, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/real time turbidimeter la 320c/product/Eiken Chemical
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
real time turbidimeter la 320c - by Bioz Stars, 2020-08
93/100 stars

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Article Title: Establishment and Application of a Multiple Cross Displacement Amplification Coupled With Nanoparticle-Based Lateral Flow Biosensor Assay for Detection of Mycoplasma pneumoniae
Article Snippet: Real-time turbidimeter LA-320C was purchased from Eiken Chemical Co., Ltd, Japan.

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    Eiken Chemical loopamp real time turbidimeter
    Relationship of turbidity with copy number of the 2009 H1N1 influenza A virus. Real‐time turbidity was detected with a <t>Loopamp</t> real‐time turbidimeter. The time required to reach the threshold turbidity level (0.1) (threshold time) were plotted against the copy numbers of the samples determined by real‐time RT‐PCR.
    Loopamp Real Time Turbidimeter, supplied by Eiken Chemical, used in various techniques. Bioz Stars score: 91/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/loopamp real time turbidimeter/product/Eiken Chemical
    Average 91 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    loopamp real time turbidimeter - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    85
    Eiken Chemical loopamp real time la 320c turbidimeter
    Sensitivity of the hMPV genotype-specific RT-LAMP assay. Serial diluted RNAs ranging from 10 −6 to 10 −13 were used to determine the detection limits of the hMPV genotype-specific RT-LAMP. The results of the genotype-specific RT-LAMP were assessed by the <t>Loopamp</t> ® real-time <t>LA-320C</t> turbidimeter.
    Loopamp Real Time La 320c Turbidimeter, supplied by Eiken Chemical, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/loopamp real time la 320c turbidimeter/product/Eiken Chemical
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    loopamp real time la 320c turbidimeter - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

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    Relationship of turbidity with copy number of the 2009 H1N1 influenza A virus. Real‐time turbidity was detected with a Loopamp real‐time turbidimeter. The time required to reach the threshold turbidity level (0.1) (threshold time) were plotted against the copy numbers of the samples determined by real‐time RT‐PCR.

    Journal: Journal of Medical Virology

    Article Title: Mobile and accurate detection system for infection by the 2009 pandemic influenza A (H1N1) virus with a pocket‐warmer reverse‐transcriptase loop‐mediated isothermal amplification

    doi: 10.1002/jmv.22031

    Figure Lengend Snippet: Relationship of turbidity with copy number of the 2009 H1N1 influenza A virus. Real‐time turbidity was detected with a Loopamp real‐time turbidimeter. The time required to reach the threshold turbidity level (0.1) (threshold time) were plotted against the copy numbers of the samples determined by real‐time RT‐PCR.

    Article Snippet: For the conventional RT‐LAMP method, 50 µl of RT‐LAMP mixture including 9 µl of RNA sample was incubated in a Loopamp Real‐Time Turbidimeter (LA‐320C; Eiken Chemical) for 60 min at 60°C and then for 5 min at 80°C to terminate the reaction.

    Techniques: Quantitative RT-PCR

    Comparison of the sensitivities for bla L1 gene detection by LAMP and conventional PCR methods. Pure genomic DNA extracted from S. maltophilia- K279a was diluted tenfold (379.0 ng/μl to 0.00379 pg/μl) and the DNA assayed by LAMP (A,B) and PCR (C) . (A) Turbidity was monitored using the Loopamp real-time turbidimeter and the OD recorded at 650 nm, at 6 s intervals. (B) Visual inspection of the color change, post-LAMP assay, and in the presence of calcein/Mn 2+ complex. (C) PCR products were analyzed by 2% agarose gel electrophoresis and stained with ethidium bromide. The DNA marker is D2000 DNA Marker (Tiangen Biotech Co., Ltd.) The size is about 179 bp.

    Journal: Frontiers in Microbiology

    Article Title: Prevalence and detection of Stenotrophomonas maltophilia carrying metallo-β-lactamase blaL1 in Beijing, China

    doi: 10.3389/fmicb.2014.00692

    Figure Lengend Snippet: Comparison of the sensitivities for bla L1 gene detection by LAMP and conventional PCR methods. Pure genomic DNA extracted from S. maltophilia- K279a was diluted tenfold (379.0 ng/μl to 0.00379 pg/μl) and the DNA assayed by LAMP (A,B) and PCR (C) . (A) Turbidity was monitored using the Loopamp real-time turbidimeter and the OD recorded at 650 nm, at 6 s intervals. (B) Visual inspection of the color change, post-LAMP assay, and in the presence of calcein/Mn 2+ complex. (C) PCR products were analyzed by 2% agarose gel electrophoresis and stained with ethidium bromide. The DNA marker is D2000 DNA Marker (Tiangen Biotech Co., Ltd.) The size is about 179 bp.

    Article Snippet: Real-time changes in turbidity were monitored by measuring the optical density (λ650 nm ) at 6 s intervals, for each LAMP reaction in a Loopamp real-time turbidimeter (LA-320c; Eiken Chemical Co., Ltd.).

    Techniques: Polymerase Chain Reaction, Lamp Assay, Agarose Gel Electrophoresis, Staining, Marker

    Specificity of the LAMP method for bla L1 gene detection. It has two parts, (A) is the graphic and (B) is the photography of microtubes. The reaction proceeded at 65°C for 65 min. Turbidity was monitored in the Loopamp real-time turbidimeter and the OD (λ650nm) recorded at 6 s intervals. L1, Brucella suis 3572; L2, Bacillus megatherium 4623; L3, Vibrio carchariae 5732; L4, Acinetobacter baumannii B260; L5, Corynebacterium diphtheriae CMCC38001; L6, Acinetobacter baumannii H18; L7, Mycobacterium tuberculosis 8362; L8, Shigella sonnei 2531; L9, Shigella flexneri 4536; L10, Salmonella enteritidis 50326-1; L11, Yersinia enterocolitica 1836; L12, Vibrio parahaemolyticus 5474; L13, Salmonella paratyphi 86423; L14, Neisseria meningitidis group B CMCC29022; L15, Enterotoxigenic E. coli 44824; L16, Beta hemolytic Streptococcus group A CMCC32213; L17, Yersinia pestis 2638; L18, Salmonella aberdeen 9264; L19, Vibrio cholera 3802; L20, Staphylococcus aureus 2740; L21, Bordetella pertussis ATCC 18530 ; L22, positive control ( S. maltophilia - K279a); L23, negative control (distilled water).

    Journal: Frontiers in Microbiology

    Article Title: Prevalence and detection of Stenotrophomonas maltophilia carrying metallo-β-lactamase blaL1 in Beijing, China

    doi: 10.3389/fmicb.2014.00692

    Figure Lengend Snippet: Specificity of the LAMP method for bla L1 gene detection. It has two parts, (A) is the graphic and (B) is the photography of microtubes. The reaction proceeded at 65°C for 65 min. Turbidity was monitored in the Loopamp real-time turbidimeter and the OD (λ650nm) recorded at 6 s intervals. L1, Brucella suis 3572; L2, Bacillus megatherium 4623; L3, Vibrio carchariae 5732; L4, Acinetobacter baumannii B260; L5, Corynebacterium diphtheriae CMCC38001; L6, Acinetobacter baumannii H18; L7, Mycobacterium tuberculosis 8362; L8, Shigella sonnei 2531; L9, Shigella flexneri 4536; L10, Salmonella enteritidis 50326-1; L11, Yersinia enterocolitica 1836; L12, Vibrio parahaemolyticus 5474; L13, Salmonella paratyphi 86423; L14, Neisseria meningitidis group B CMCC29022; L15, Enterotoxigenic E. coli 44824; L16, Beta hemolytic Streptococcus group A CMCC32213; L17, Yersinia pestis 2638; L18, Salmonella aberdeen 9264; L19, Vibrio cholera 3802; L20, Staphylococcus aureus 2740; L21, Bordetella pertussis ATCC 18530 ; L22, positive control ( S. maltophilia - K279a); L23, negative control (distilled water).

    Article Snippet: Real-time changes in turbidity were monitored by measuring the optical density (λ650 nm ) at 6 s intervals, for each LAMP reaction in a Loopamp real-time turbidimeter (LA-320c; Eiken Chemical Co., Ltd.).

    Techniques: Positive Control, Negative Control

    Loop-mediated isothermal amplification results for 15 S. maltophilia strains positive for bla L1 isolated from 15 clinical samples. (A) Turbidity was monitored using Loopamp, and the OD measured at 650 nm every 6 s. (B) Visual inspection of calcein/Mn 2+ complex associated color changes post-LAMP assay. 1, S. maltophilia -2; 2, S. maltophilia -17; 3, S. maltophilia -24; 4, S. maltophilia -25; 5, S. maltophilia -36; 6, S. maltophilia -41; 7, S. maltophilia -51; 8, S. maltophilia -58; 9, S. maltophilia -63; 10, S. maltophilia -65; 11, S. maltophilia -66; 12, S. maltophilia -67; 13, S. maltophilia -3859; 14, S. maltophilia -4621; 15, S. maltophilia -WJ2; 16, positive control ( S. maltophilia- K279a); 17, negative control (distilled water).

    Journal: Frontiers in Microbiology

    Article Title: Prevalence and detection of Stenotrophomonas maltophilia carrying metallo-β-lactamase blaL1 in Beijing, China

    doi: 10.3389/fmicb.2014.00692

    Figure Lengend Snippet: Loop-mediated isothermal amplification results for 15 S. maltophilia strains positive for bla L1 isolated from 15 clinical samples. (A) Turbidity was monitored using Loopamp, and the OD measured at 650 nm every 6 s. (B) Visual inspection of calcein/Mn 2+ complex associated color changes post-LAMP assay. 1, S. maltophilia -2; 2, S. maltophilia -17; 3, S. maltophilia -24; 4, S. maltophilia -25; 5, S. maltophilia -36; 6, S. maltophilia -41; 7, S. maltophilia -51; 8, S. maltophilia -58; 9, S. maltophilia -63; 10, S. maltophilia -65; 11, S. maltophilia -66; 12, S. maltophilia -67; 13, S. maltophilia -3859; 14, S. maltophilia -4621; 15, S. maltophilia -WJ2; 16, positive control ( S. maltophilia- K279a); 17, negative control (distilled water).

    Article Snippet: Real-time changes in turbidity were monitored by measuring the optical density (λ650 nm ) at 6 s intervals, for each LAMP reaction in a Loopamp real-time turbidimeter (LA-320c; Eiken Chemical Co., Ltd.).

    Techniques: Amplification, Isolation, Lamp Assay, Positive Control, Negative Control

    Sensitivity of the hMPV genotype-specific RT-LAMP assay. Serial diluted RNAs ranging from 10 −6 to 10 −13 were used to determine the detection limits of the hMPV genotype-specific RT-LAMP. The results of the genotype-specific RT-LAMP were assessed by the Loopamp ® real-time LA-320C turbidimeter.

    Journal: Journal of Virological Methods

    Article Title: Identification of human metapneumovirus genotypes A and B from clinical specimens by reverse transcription loop-mediated isothermal amplification

    doi: 10.1016/j.jviromet.2013.10.037

    Figure Lengend Snippet: Sensitivity of the hMPV genotype-specific RT-LAMP assay. Serial diluted RNAs ranging from 10 −6 to 10 −13 were used to determine the detection limits of the hMPV genotype-specific RT-LAMP. The results of the genotype-specific RT-LAMP were assessed by the Loopamp ® real-time LA-320C turbidimeter.

    Article Snippet: A Loopamp® real-time LA-320C turbidimeter (Eiken Chemical, Tokyo, Japan) was used to monitor the accumulation of magnesium pyrophosphate spectrophotometrically at 650 nm.

    Techniques: RT Lamp Assay