real time pcr  (Bio-Rad)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    MiniOpticon Real Time PCR Detection System
    Description:
    Modular thermal cycler platform includes optical housing MJ Mini Thermal Cycler analysis software
    Catalog Number:
    3591592
    Price:
    None
    Category:
    VINEO Brettanomytest PCR Kit
    Buy from Supplier


    Structured Review

    Bio-Rad real time pcr
    MiniOpticon Real Time PCR Detection System
    Modular thermal cycler platform includes optical housing MJ Mini Thermal Cycler analysis software
    https://www.bioz.com/result/real time pcr/product/Bio-Rad
    Average 99 stars, based on 9081 article reviews
    Price from $9.99 to $1999.99
    real time pcr - by Bioz Stars, 2021-01
    99/100 stars

    Images

    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: The Homeodomain Protein Ladybird Late Regulates Synthesis of Milk Proteins during Pregnancy in the Tsetse Fly (Glossina morsitans)
    Article Snippet: .. Levels of mgp1 were determined by CFX Connect Real Time PCR Detection System with SYBR Green Supermix (Bio-Rad, Hercules, CA). .. The data were analyzed with software version 3.1 (Bio-Rad).

    Article Title: TGF-β1 Upregulates the Expression of Triggering Receptor Expressed on Myeloid Cells 1 in Murine Lungs
    Article Snippet: .. Transcriptional levels of procollagen types I and III, TGF-β1, TREM-1, and AP-1 were determined using UltraSYBR Mixture (cwbiotech, China) and a Real-time PCR Detection System (Bio-Rad, USA). .. The mRNA expression of our genes of interest was calculated by 2−ΔΔCt method normalized to housekeeper GAPDH.

    Article Title: Use of Genomic DNA as an Indirect Reference for Identifying Gender-Associated Transcripts in Morphologically Identical, but Chromosomally Distinct, Schistosoma mansoni Cercariae
    Article Snippet: .. Real time PCR analysis Relative gene expression (fold difference between samples) was quantified relative to alpha-tubulin using a MiniOpticon Real-Time PCR Detection System (Bio-Rad) and SYBR green chemistry (Bio-Rad). .. Total reaction volume was 25 uL using 250 nM of each primer (sequences provided in ) and 2 uL of 1/10 dilution from the original cDNA microarray targets (before labeling).

    Article Title: Large-scale profiling of noncoding RNA function in yeast
    Article Snippet: .. Quantitative RT-PCR was performed on the cDNA using iTaq universal SYBR green Supermix (BioRad) in a CFX Connect Real-time PCR Detection System (BioRad). qPCR cycling conditions were as follows: initial denaturation 95°C for 3 mins; 35 cycles of 95°C for 45 secs, 58°C for 45 secs and 72°C for 3 mins; final extension of 72°C for 5 mins. ..

    Article Title: Molecular mechanism(s) involved in differential expression of vitamin C transporters along the intestinal tract
    Article Snippet: .. Quantitative real-time PCR (RT-qPCR) was performed using the SYBR Green PCR kit and a real-time PCR detection system (model CFX96, Bio-Rad). ..

    Article Title: ADAR2-mediated Q/R editing of GluK2 regulates kainate receptor upscaling in response to suppression of synaptic activity
    Article Snippet: .. For quantitative PCR (RT-qPCR), 2 µl of the cDNA samples per condition were mixed with PowerUp SYBR Green Master Mix (Life Technologies) and forward and reverse primers targeting ADAR2 and GAPDH, and amplified quantitatively using a real-time PCR System (MiniOpticon, BioRAD) for 40 cycles and Ct values were recorded. ..

    Article Title: The potential role of lysosome-associated membrane protein 3 (LAMP3) on cardiac remodelling
    Article Snippet: .. The selected gene mRNA levels were detected by CFX Connect™ Real-Time PCR Detection System (Bio-Rad) using iQ™SYBR® Green Supermix (1708884, Bio-Rad) and results were normalized against GAPDH gene expression. .. The immunohistochemistry analysis for LAMP3 was followed the standard protocol with minor changes.

    Article Title: Angiogenesis in liquid tumors: An in-vitro assay for leukemic cell induced bone marrow angiogenesis
    Article Snippet: .. RT-PCR was conducted using the CFX connect real-time PCR detection system (Bio-rad) using the following primers: bFGF (forward, 5′-AGAGCGACCCTCACATCAAG-3′; and reverse, 5′-TCGTTTCAGTGCCACATACC-3′), and VEGF (forward, 5′-CTACCTCCACCATGCCAAGT-3′; and reverse, 5′-GCAGTAGCTGCGCTGATAGA-3′). .. Measurement results were compared using independent, two-tailed Student t -test in Excel (Microsoft).

    Amplification:

    Article Title: ADAR2-mediated Q/R editing of GluK2 regulates kainate receptor upscaling in response to suppression of synaptic activity
    Article Snippet: .. For quantitative PCR (RT-qPCR), 2 µl of the cDNA samples per condition were mixed with PowerUp SYBR Green Master Mix (Life Technologies) and forward and reverse primers targeting ADAR2 and GAPDH, and amplified quantitatively using a real-time PCR System (MiniOpticon, BioRAD) for 40 cycles and Ct values were recorded. ..

    Quantitative RT-PCR:

    Article Title: Large-scale profiling of noncoding RNA function in yeast
    Article Snippet: .. Quantitative RT-PCR was performed on the cDNA using iTaq universal SYBR green Supermix (BioRad) in a CFX Connect Real-time PCR Detection System (BioRad). qPCR cycling conditions were as follows: initial denaturation 95°C for 3 mins; 35 cycles of 95°C for 45 secs, 58°C for 45 secs and 72°C for 3 mins; final extension of 72°C for 5 mins. ..

    Article Title: Molecular mechanism(s) involved in differential expression of vitamin C transporters along the intestinal tract
    Article Snippet: .. Quantitative real-time PCR (RT-qPCR) was performed using the SYBR Green PCR kit and a real-time PCR detection system (model CFX96, Bio-Rad). ..

    Article Title: ADAR2-mediated Q/R editing of GluK2 regulates kainate receptor upscaling in response to suppression of synaptic activity
    Article Snippet: .. For quantitative PCR (RT-qPCR), 2 µl of the cDNA samples per condition were mixed with PowerUp SYBR Green Master Mix (Life Technologies) and forward and reverse primers targeting ADAR2 and GAPDH, and amplified quantitatively using a real-time PCR System (MiniOpticon, BioRAD) for 40 cycles and Ct values were recorded. ..

    SYBR Green Assay:

    Article Title: The Homeodomain Protein Ladybird Late Regulates Synthesis of Milk Proteins during Pregnancy in the Tsetse Fly (Glossina morsitans)
    Article Snippet: .. Levels of mgp1 were determined by CFX Connect Real Time PCR Detection System with SYBR Green Supermix (Bio-Rad, Hercules, CA). .. The data were analyzed with software version 3.1 (Bio-Rad).

    Article Title: Use of Genomic DNA as an Indirect Reference for Identifying Gender-Associated Transcripts in Morphologically Identical, but Chromosomally Distinct, Schistosoma mansoni Cercariae
    Article Snippet: .. Real time PCR analysis Relative gene expression (fold difference between samples) was quantified relative to alpha-tubulin using a MiniOpticon Real-Time PCR Detection System (Bio-Rad) and SYBR green chemistry (Bio-Rad). .. Total reaction volume was 25 uL using 250 nM of each primer (sequences provided in ) and 2 uL of 1/10 dilution from the original cDNA microarray targets (before labeling).

    Article Title: Large-scale profiling of noncoding RNA function in yeast
    Article Snippet: .. Quantitative RT-PCR was performed on the cDNA using iTaq universal SYBR green Supermix (BioRad) in a CFX Connect Real-time PCR Detection System (BioRad). qPCR cycling conditions were as follows: initial denaturation 95°C for 3 mins; 35 cycles of 95°C for 45 secs, 58°C for 45 secs and 72°C for 3 mins; final extension of 72°C for 5 mins. ..

    Article Title: Molecular mechanism(s) involved in differential expression of vitamin C transporters along the intestinal tract
    Article Snippet: .. Quantitative real-time PCR (RT-qPCR) was performed using the SYBR Green PCR kit and a real-time PCR detection system (model CFX96, Bio-Rad). ..

    Article Title: ADAR2-mediated Q/R editing of GluK2 regulates kainate receptor upscaling in response to suppression of synaptic activity
    Article Snippet: .. For quantitative PCR (RT-qPCR), 2 µl of the cDNA samples per condition were mixed with PowerUp SYBR Green Master Mix (Life Technologies) and forward and reverse primers targeting ADAR2 and GAPDH, and amplified quantitatively using a real-time PCR System (MiniOpticon, BioRAD) for 40 cycles and Ct values were recorded. ..

    Polymerase Chain Reaction:

    Article Title: Molecular mechanism(s) involved in differential expression of vitamin C transporters along the intestinal tract
    Article Snippet: .. Quantitative real-time PCR (RT-qPCR) was performed using the SYBR Green PCR kit and a real-time PCR detection system (model CFX96, Bio-Rad). ..

    Expressing:

    Article Title: Use of Genomic DNA as an Indirect Reference for Identifying Gender-Associated Transcripts in Morphologically Identical, but Chromosomally Distinct, Schistosoma mansoni Cercariae
    Article Snippet: .. Real time PCR analysis Relative gene expression (fold difference between samples) was quantified relative to alpha-tubulin using a MiniOpticon Real-Time PCR Detection System (Bio-Rad) and SYBR green chemistry (Bio-Rad). .. Total reaction volume was 25 uL using 250 nM of each primer (sequences provided in ) and 2 uL of 1/10 dilution from the original cDNA microarray targets (before labeling).

    Article Title: The potential role of lysosome-associated membrane protein 3 (LAMP3) on cardiac remodelling
    Article Snippet: .. The selected gene mRNA levels were detected by CFX Connect™ Real-Time PCR Detection System (Bio-Rad) using iQ™SYBR® Green Supermix (1708884, Bio-Rad) and results were normalized against GAPDH gene expression. .. The immunohistochemistry analysis for LAMP3 was followed the standard protocol with minor changes.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Angiogenesis in liquid tumors: An in-vitro assay for leukemic cell induced bone marrow angiogenesis
    Article Snippet: .. RT-PCR was conducted using the CFX connect real-time PCR detection system (Bio-rad) using the following primers: bFGF (forward, 5′-AGAGCGACCCTCACATCAAG-3′; and reverse, 5′-TCGTTTCAGTGCCACATACC-3′), and VEGF (forward, 5′-CTACCTCCACCATGCCAAGT-3′; and reverse, 5′-GCAGTAGCTGCGCTGATAGA-3′). .. Measurement results were compared using independent, two-tailed Student t -test in Excel (Microsoft).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92
    Bio-Rad quantitative real time reverse transcription rt pcr purified total rna
    Pearson correlation value between the log2-transformed fold changes of <t>RNA-Seq</t> data and <t>qRT-PCR</t> validation. Each dot (in blue) represents a data point for the selected genes and each corresponding sample (4 genes and 8 replicates in control (D0) and later time point, D1 and D14, respectively). And the dashed lines are 95% confidence interval.
    Quantitative Real Time Reverse Transcription Rt Pcr Purified Total Rna, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantitative real time reverse transcription rt pcr purified total rna/product/Bio-Rad
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    quantitative real time reverse transcription rt pcr purified total rna - by Bioz Stars, 2021-01
    92/100 stars
      Buy from Supplier

    92
    Bio-Rad real time pcr analysis total rna
    High temperature induces endoplasmic reticulum (ER) stress-mediated cell death. ( a ) Protein expression under condition of hyperthermia was measured. Cell lysates were collected at indicated times following incubation at 42 °C or tunicamycin (Tm) (1 μg/mL) treatment for western blot analysis. ( b ) Effect of heat stress on the unfolded protein response (UPR) gene expression. Quantitative <t>RT-PCR</t> was performed using total <t>RNA</t> extracted from mouse embryonic fibroblasts (MEFs) which were incubated at 42 °C for indicated time periods. Data are presented as means ± SEM (n = 3 independent experiments). ( c ) KDEL and DAPI immunofluorescence staining. KDEL and DAPI staining were performed using MEFs incubated at 42 °C or treated with thapsigargin (Tg) (300 nM) for 12 h. Representative images are shown. Scale bar represents 100 μm. ( d ) Cell viability was measured in MEFs treated with Tg (300 nM), Tm (1 μg/mL), or incubated at 42 °C for the indicated time periods. ( e ) Cell viability was measured in MEFs which were incubated at 42 °C for indicated time periods in the presence or absence of tauroursodeoxycholic acid (TUDCA) (2.4 mM). ** p
    Real Time Pcr Analysis Total Rna, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time pcr analysis total rna/product/Bio-Rad
    Average 92 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    real time pcr analysis total rna - by Bioz Stars, 2021-01
    92/100 stars
      Buy from Supplier

    85
    Bio-Rad arginase 1 real time pcr real time pcr
    Myeloid derived suppressor cell (MDSC) and <t>arginase</t> 1 (Arg1) levels. (A) Levels of MDSCs (CD11b+CD14−CD33+) in different CML patient risk groups (high risk (HR) n = 10, low risk (LR) n = 7) and healthy control subjects (HC, n = 21) as shown by flow cytometry. (B) Levels of MDSCs expressing CD34 in CML patient risk groups (HR n = 10, LR n = 7) and HCs (n = 21). (C) Arg1 mRNA expression in HCs (n = 9) and CML patients (HR n = 4, LR n = 2) assessed by real time <t>PCR.</t> Statistically significant differences between groups are indicated by P-values in the figures. (D) Arg1 concentration in CML patient plasma (HR n = 7, LR n = 3) measured by ELISA. (E) Arg 1 mRNA expression in CML cell lines. The plot shows mean values with standard error of the mean.
    Arginase 1 Real Time Pcr Real Time Pcr, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/arginase 1 real time pcr real time pcr/product/Bio-Rad
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    arginase 1 real time pcr real time pcr - by Bioz Stars, 2021-01
    85/100 stars
      Buy from Supplier

    85
    Bio-Rad genes quantitative real time rt pcr
    Genome organizations of HLSV and TMV and detection of viral <t>RNA</t> and protein levels in cross protection. ( A ) Genome organization of HLSV and TMV. Transcriptional level of HLSV ( B ) or TMV ( C ) gRNA/total viral RNA determined by quantitative real-time <t>RT-PCR</t> and translational level of CPs by western blot ( B and C ). Significant differences were calculated using the Student’s t -test, * and ** indicate significance at the 0.05 and 0.01 levels of confidence, respectively.
    Genes Quantitative Real Time Rt Pcr, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genes quantitative real time rt pcr/product/Bio-Rad
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    genes quantitative real time rt pcr - by Bioz Stars, 2021-01
    85/100 stars
      Buy from Supplier

    Image Search Results


    Pearson correlation value between the log2-transformed fold changes of RNA-Seq data and qRT-PCR validation. Each dot (in blue) represents a data point for the selected genes and each corresponding sample (4 genes and 8 replicates in control (D0) and later time point, D1 and D14, respectively). And the dashed lines are 95% confidence interval.

    Journal: Scientific Reports

    Article Title: Temporal dynamics in meta longitudinal RNA-Seq data

    doi: 10.1038/s41598-018-37397-7

    Figure Lengend Snippet: Pearson correlation value between the log2-transformed fold changes of RNA-Seq data and qRT-PCR validation. Each dot (in blue) represents a data point for the selected genes and each corresponding sample (4 genes and 8 replicates in control (D0) and later time point, D1 and D14, respectively). And the dashed lines are 95% confidence interval.

    Article Snippet: Quantitative Real-time Reverse Transcription (RT) PCR Purified total RNA samples were converted to cDNA using an iScript Advanced cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA).

    Techniques: Transformation Assay, RNA Sequencing Assay, Quantitative RT-PCR

    High temperature induces endoplasmic reticulum (ER) stress-mediated cell death. ( a ) Protein expression under condition of hyperthermia was measured. Cell lysates were collected at indicated times following incubation at 42 °C or tunicamycin (Tm) (1 μg/mL) treatment for western blot analysis. ( b ) Effect of heat stress on the unfolded protein response (UPR) gene expression. Quantitative RT-PCR was performed using total RNA extracted from mouse embryonic fibroblasts (MEFs) which were incubated at 42 °C for indicated time periods. Data are presented as means ± SEM (n = 3 independent experiments). ( c ) KDEL and DAPI immunofluorescence staining. KDEL and DAPI staining were performed using MEFs incubated at 42 °C or treated with thapsigargin (Tg) (300 nM) for 12 h. Representative images are shown. Scale bar represents 100 μm. ( d ) Cell viability was measured in MEFs treated with Tg (300 nM), Tm (1 μg/mL), or incubated at 42 °C for the indicated time periods. ( e ) Cell viability was measured in MEFs which were incubated at 42 °C for indicated time periods in the presence or absence of tauroursodeoxycholic acid (TUDCA) (2.4 mM). ** p

    Journal: Cells

    Article Title: Modulation of Protein Synthesis by eIF2α Phosphorylation Protects Cell from Heat Stress-Mediated Apoptosis

    doi: 10.3390/cells7120254

    Figure Lengend Snippet: High temperature induces endoplasmic reticulum (ER) stress-mediated cell death. ( a ) Protein expression under condition of hyperthermia was measured. Cell lysates were collected at indicated times following incubation at 42 °C or tunicamycin (Tm) (1 μg/mL) treatment for western blot analysis. ( b ) Effect of heat stress on the unfolded protein response (UPR) gene expression. Quantitative RT-PCR was performed using total RNA extracted from mouse embryonic fibroblasts (MEFs) which were incubated at 42 °C for indicated time periods. Data are presented as means ± SEM (n = 3 independent experiments). ( c ) KDEL and DAPI immunofluorescence staining. KDEL and DAPI staining were performed using MEFs incubated at 42 °C or treated with thapsigargin (Tg) (300 nM) for 12 h. Representative images are shown. Scale bar represents 100 μm. ( d ) Cell viability was measured in MEFs treated with Tg (300 nM), Tm (1 μg/mL), or incubated at 42 °C for the indicated time periods. ( e ) Cell viability was measured in MEFs which were incubated at 42 °C for indicated time periods in the presence or absence of tauroursodeoxycholic acid (TUDCA) (2.4 mM). ** p

    Article Snippet: RNA Extraction and Real-Time PCR Analysis Total RNA was extracted from cells using Trizol (Gene All, Ribo-Ex, Seoul, Korea) and cDNA was synthesized using an iScript cDNA synthesis kit (Bio-Rad, Hercules, CA, USA, BR170-8891).

    Techniques: Expressing, Incubation, Western Blot, Quantitative RT-PCR, Immunofluorescence, Staining

    The ATF6α pathway does not protect cells from heat stress-mediated death. ( a ) Quantitative RT-PCR was performed using total RNA extracted from Atf6α −/− and Atf6α +/+ MEFs which were incubated at 42 °C for indicated time periods. Data are presented as means ± SEM (n = 3 independent experiments). ( b ) Protein expression under conditions of hyperthermia was measured. Following 42 °C incubation, cell lysates were collected at indicated times for western blot analysis. ( c ) Cell viability was measured in Atf6α −/− and Atf6α +/+ MEFs following incubation at 42 °C for indicated time periods. ( d ) Caspase 3/7 activity was detected in Atf6α −/− and Atf6α +/+ MEFs which were incubated at 42 °C for 12 h. ** p

    Journal: Cells

    Article Title: Modulation of Protein Synthesis by eIF2α Phosphorylation Protects Cell from Heat Stress-Mediated Apoptosis

    doi: 10.3390/cells7120254

    Figure Lengend Snippet: The ATF6α pathway does not protect cells from heat stress-mediated death. ( a ) Quantitative RT-PCR was performed using total RNA extracted from Atf6α −/− and Atf6α +/+ MEFs which were incubated at 42 °C for indicated time periods. Data are presented as means ± SEM (n = 3 independent experiments). ( b ) Protein expression under conditions of hyperthermia was measured. Following 42 °C incubation, cell lysates were collected at indicated times for western blot analysis. ( c ) Cell viability was measured in Atf6α −/− and Atf6α +/+ MEFs following incubation at 42 °C for indicated time periods. ( d ) Caspase 3/7 activity was detected in Atf6α −/− and Atf6α +/+ MEFs which were incubated at 42 °C for 12 h. ** p

    Article Snippet: RNA Extraction and Real-Time PCR Analysis Total RNA was extracted from cells using Trizol (Gene All, Ribo-Ex, Seoul, Korea) and cDNA was synthesized using an iScript cDNA synthesis kit (Bio-Rad, Hercules, CA, USA, BR170-8891).

    Techniques: Quantitative RT-PCR, Incubation, Expressing, Western Blot, Activity Assay

    eIF2α phosphorylation is required to protect cells from heat stress-mediated death. ( a ) Protein expression was measured under conditions of hyperthermia. Cell lysates were collected from Eif2α S/S and Eif2α A/A MEFs at indicated times for western blot analysis following incubation at 42 °C. ( b ) Quantitative RT-PCR was performed using total RNA extracted from Eif2α S/S and Eif2α A/A MEFs which were incubated at 42 °C for indicated time periods. Data are presented as means ± SEM (n = 3 independent experiments). ( c ) Cell viability was measured in Eif2α S/S and Eif2α A/A MEFs which were incubated at 42 °C for indicated times. ( d ) Caspase 3/7 activity was detected in Eif2α S/S and Eif2α A/A MEFs which were incubated at 42 °C for 12 h. ( e ) Cell viability was measured in MEFs which were incubated at 42 °C for indicated time periods in the presence or absence of guanabenz (GA, 1 μM) or ISRIB (5 μM). ( f ) Cell viability was measured in Perk +/+ and Perk −/− MEFs which were incubated at 42 °C for 12 and 24 h. ( g ) Cell viability was measured in MEFs which were incubated at 42 °C for 12 h in the presence or absence of GSK2606414 (100 nM). * p

    Journal: Cells

    Article Title: Modulation of Protein Synthesis by eIF2α Phosphorylation Protects Cell from Heat Stress-Mediated Apoptosis

    doi: 10.3390/cells7120254

    Figure Lengend Snippet: eIF2α phosphorylation is required to protect cells from heat stress-mediated death. ( a ) Protein expression was measured under conditions of hyperthermia. Cell lysates were collected from Eif2α S/S and Eif2α A/A MEFs at indicated times for western blot analysis following incubation at 42 °C. ( b ) Quantitative RT-PCR was performed using total RNA extracted from Eif2α S/S and Eif2α A/A MEFs which were incubated at 42 °C for indicated time periods. Data are presented as means ± SEM (n = 3 independent experiments). ( c ) Cell viability was measured in Eif2α S/S and Eif2α A/A MEFs which were incubated at 42 °C for indicated times. ( d ) Caspase 3/7 activity was detected in Eif2α S/S and Eif2α A/A MEFs which were incubated at 42 °C for 12 h. ( e ) Cell viability was measured in MEFs which were incubated at 42 °C for indicated time periods in the presence or absence of guanabenz (GA, 1 μM) or ISRIB (5 μM). ( f ) Cell viability was measured in Perk +/+ and Perk −/− MEFs which were incubated at 42 °C for 12 and 24 h. ( g ) Cell viability was measured in MEFs which were incubated at 42 °C for 12 h in the presence or absence of GSK2606414 (100 nM). * p

    Article Snippet: RNA Extraction and Real-Time PCR Analysis Total RNA was extracted from cells using Trizol (Gene All, Ribo-Ex, Seoul, Korea) and cDNA was synthesized using an iScript cDNA synthesis kit (Bio-Rad, Hercules, CA, USA, BR170-8891).

    Techniques: Expressing, Western Blot, Incubation, Quantitative RT-PCR, Activity Assay

    The IRE1α pathway does not protect cells from heat stress-mediated death. ( a ) Quantitative RT-PCR was performed using total RNA, extracted from Ire1α −/− and Ire1α +/+ MEFs which were incubated at 42 °C for indicated time periods. Data are presented as means ± SEM. (n = 3 independent experiments). ( b ) Protein expression under conditions of hyperthermia was measured. Following incubation at 42 °C, cell lysates were collected at the indicated times for western blot analysis. ( c ) Cell viability was measured in Ire1α −/− and Ire1α +/+ MEFs which were incubated at 42 °C for indicated time periods. ( d ) Caspase 3/7 activity was detected in Ire1α −/− and Ire1α +/+ MEFs which were incubated at 42 °C for 12 h. ( e ) Expression of sXBP1 (spliced form) and tXBP (total form) were measured using MEFs treated with 4μ8c at indicated doses in the presence or absence of Tg (300 nM) for 12 h. ( f ) Protein expression under conditions of hyperthermia was measured in the presence or absence of 4μ8c (20 μM). ( g ) Cell viability was measured in Ire1α −/− and Ire1α +/+ MEFs which were incubated at 42 °C for indicated time periods in the presence or absence of 4μ8c (20 μM). * p

    Journal: Cells

    Article Title: Modulation of Protein Synthesis by eIF2α Phosphorylation Protects Cell from Heat Stress-Mediated Apoptosis

    doi: 10.3390/cells7120254

    Figure Lengend Snippet: The IRE1α pathway does not protect cells from heat stress-mediated death. ( a ) Quantitative RT-PCR was performed using total RNA, extracted from Ire1α −/− and Ire1α +/+ MEFs which were incubated at 42 °C for indicated time periods. Data are presented as means ± SEM. (n = 3 independent experiments). ( b ) Protein expression under conditions of hyperthermia was measured. Following incubation at 42 °C, cell lysates were collected at the indicated times for western blot analysis. ( c ) Cell viability was measured in Ire1α −/− and Ire1α +/+ MEFs which were incubated at 42 °C for indicated time periods. ( d ) Caspase 3/7 activity was detected in Ire1α −/− and Ire1α +/+ MEFs which were incubated at 42 °C for 12 h. ( e ) Expression of sXBP1 (spliced form) and tXBP (total form) were measured using MEFs treated with 4μ8c at indicated doses in the presence or absence of Tg (300 nM) for 12 h. ( f ) Protein expression under conditions of hyperthermia was measured in the presence or absence of 4μ8c (20 μM). ( g ) Cell viability was measured in Ire1α −/− and Ire1α +/+ MEFs which were incubated at 42 °C for indicated time periods in the presence or absence of 4μ8c (20 μM). * p

    Article Snippet: RNA Extraction and Real-Time PCR Analysis Total RNA was extracted from cells using Trizol (Gene All, Ribo-Ex, Seoul, Korea) and cDNA was synthesized using an iScript cDNA synthesis kit (Bio-Rad, Hercules, CA, USA, BR170-8891).

    Techniques: Quantitative RT-PCR, Incubation, Expressing, Western Blot, Activity Assay

    Myeloid derived suppressor cell (MDSC) and arginase 1 (Arg1) levels. (A) Levels of MDSCs (CD11b+CD14−CD33+) in different CML patient risk groups (high risk (HR) n = 10, low risk (LR) n = 7) and healthy control subjects (HC, n = 21) as shown by flow cytometry. (B) Levels of MDSCs expressing CD34 in CML patient risk groups (HR n = 10, LR n = 7) and HCs (n = 21). (C) Arg1 mRNA expression in HCs (n = 9) and CML patients (HR n = 4, LR n = 2) assessed by real time PCR. Statistically significant differences between groups are indicated by P-values in the figures. (D) Arg1 concentration in CML patient plasma (HR n = 7, LR n = 3) measured by ELISA. (E) Arg 1 mRNA expression in CML cell lines. The plot shows mean values with standard error of the mean.

    Journal: PLoS ONE

    Article Title: Increased Level of Myeloid-Derived Suppressor Cells, Programmed Death Receptor Ligand 1/Programmed Death Receptor 1, and Soluble CD25 in Sokal High Risk Chronic Myeloid Leukemia

    doi: 10.1371/journal.pone.0055818

    Figure Lengend Snippet: Myeloid derived suppressor cell (MDSC) and arginase 1 (Arg1) levels. (A) Levels of MDSCs (CD11b+CD14−CD33+) in different CML patient risk groups (high risk (HR) n = 10, low risk (LR) n = 7) and healthy control subjects (HC, n = 21) as shown by flow cytometry. (B) Levels of MDSCs expressing CD34 in CML patient risk groups (HR n = 10, LR n = 7) and HCs (n = 21). (C) Arg1 mRNA expression in HCs (n = 9) and CML patients (HR n = 4, LR n = 2) assessed by real time PCR. Statistically significant differences between groups are indicated by P-values in the figures. (D) Arg1 concentration in CML patient plasma (HR n = 7, LR n = 3) measured by ELISA. (E) Arg 1 mRNA expression in CML cell lines. The plot shows mean values with standard error of the mean.

    Article Snippet: Arginase 1 real time PCR Real time PCR was performed on cDNA prepared from CML cell lines and leukocytes from CML patients (n = 6, patients 11, 24–27 and 32 in ) and control subjects (n = 9) with the CFX96 Real-Time System (BioRad).

    Techniques: Derivative Assay, Flow Cytometry, Cytometry, Expressing, Real-time Polymerase Chain Reaction, Concentration Assay, Enzyme-linked Immunosorbent Assay

    Genome organizations of HLSV and TMV and detection of viral RNA and protein levels in cross protection. ( A ) Genome organization of HLSV and TMV. Transcriptional level of HLSV ( B ) or TMV ( C ) gRNA/total viral RNA determined by quantitative real-time RT-PCR and translational level of CPs by western blot ( B and C ). Significant differences were calculated using the Student’s t -test, * and ** indicate significance at the 0.05 and 0.01 levels of confidence, respectively.

    Journal: PLoS ONE

    Article Title: Profiling of Genes Related to Cross Protection and Competition for NbTOM1 by HLSV and TMV

    doi: 10.1371/journal.pone.0073725

    Figure Lengend Snippet: Genome organizations of HLSV and TMV and detection of viral RNA and protein levels in cross protection. ( A ) Genome organization of HLSV and TMV. Transcriptional level of HLSV ( B ) or TMV ( C ) gRNA/total viral RNA determined by quantitative real-time RT-PCR and translational level of CPs by western blot ( B and C ). Significant differences were calculated using the Student’s t -test, * and ** indicate significance at the 0.05 and 0.01 levels of confidence, respectively.

    Article Snippet: Verification of microarray data and time course study of selected genes Quantitative real-time RT-PCR was performed to determine viral RNA levels and to investigate the expression of selected host genes of interest, using KAPA SYBR® FAST universal qPCR kit and CFX384TM Real time PCR Detection system (Bio-Rad).

    Techniques: Quantitative RT-PCR, Western Blot

    NbTOM1 transcript levels and virus accumulation with overexpression or silencing of NbTOM1 in Nicotiana benthamiana . (A) The transcriptional levels of NbTOM1 were detected in mock inoculation buffer, HLSV, TMV, HLSV+TMV (100:1) and HLSV+TMV (1:1) co-infected plants. The viral RNA levels of HLSV and TMV were determined using quantitative real-time RT-PCR with primer sequences corresponding to the coat protein genes in HLSV, or TMV or co-infected leaves. (B and C) The transcriptional levels of NbTOM1 were detected in NbTOM1 overexpressed or silenced leaves. The viral RNA levels were detected in plants first infiltrated with pGreen orpGreen- NbTOM1 (for overexpression), and pGreen or pGreen- NbTOM1 (nt1-581) (for silencing), followed by single virus (HLSV or TMV) infection or co-infection(HLSV+TMV) at 40 h post inoculation (hpi). (D) The coat proteins of HLSV and TMV were detected by western blot in NbTOM1 overexpressed or silenced leaves which were subsequently infected with single virus (HLSV or TMV) or co-infected with HLSV+TMV at 5 dpi (details see Materials Methods). Total protein from mock buffer inoculated N . benthamiana leaves was used as the negative control, while the total protein from HLSV or TMV infected leave samples which were confirmed earlier were used as positive controls.

    Journal: PLoS ONE

    Article Title: Profiling of Genes Related to Cross Protection and Competition for NbTOM1 by HLSV and TMV

    doi: 10.1371/journal.pone.0073725

    Figure Lengend Snippet: NbTOM1 transcript levels and virus accumulation with overexpression or silencing of NbTOM1 in Nicotiana benthamiana . (A) The transcriptional levels of NbTOM1 were detected in mock inoculation buffer, HLSV, TMV, HLSV+TMV (100:1) and HLSV+TMV (1:1) co-infected plants. The viral RNA levels of HLSV and TMV were determined using quantitative real-time RT-PCR with primer sequences corresponding to the coat protein genes in HLSV, or TMV or co-infected leaves. (B and C) The transcriptional levels of NbTOM1 were detected in NbTOM1 overexpressed or silenced leaves. The viral RNA levels were detected in plants first infiltrated with pGreen orpGreen- NbTOM1 (for overexpression), and pGreen or pGreen- NbTOM1 (nt1-581) (for silencing), followed by single virus (HLSV or TMV) infection or co-infection(HLSV+TMV) at 40 h post inoculation (hpi). (D) The coat proteins of HLSV and TMV were detected by western blot in NbTOM1 overexpressed or silenced leaves which were subsequently infected with single virus (HLSV or TMV) or co-infected with HLSV+TMV at 5 dpi (details see Materials Methods). Total protein from mock buffer inoculated N . benthamiana leaves was used as the negative control, while the total protein from HLSV or TMV infected leave samples which were confirmed earlier were used as positive controls.

    Article Snippet: Verification of microarray data and time course study of selected genes Quantitative real-time RT-PCR was performed to determine viral RNA levels and to investigate the expression of selected host genes of interest, using KAPA SYBR® FAST universal qPCR kit and CFX384TM Real time PCR Detection system (Bio-Rad).

    Techniques: Over Expression, Infection, Quantitative RT-PCR, Western Blot, Negative Control