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TaKaRa real time pcr assay total rna
MTB Hsp16.3 induces mouse bone marrow-derived macrophage (BMDM) M2 polarization. Bone marrow cells were isolated from the tibias and femurs of BALB/c mice (6–8 weeks old) and incubated with 20 ng/ml GM-CSF for 7 days. Then, BMDMs were treated with 100 ng/ml MTB Hsp16.3, 100 ng/ml IFN-γ, or 100 ng/ml IL-4 for 0–72 h. a The morphology of BMDMs incubated with IFN-γ, IL-4, or MTB Hsp16.3 for 12 h. The images were captured under an inverted microscope (× 200); the picture in the upper right corner is × 400. Total <t>RNA</t> was extracted from the cells using TRIzol according to the manufacturer’s instructions. b The mRNA expression levels of iNOS, IL-6, TNF-α, Arg-1, IL-10, and TGF-β in macrophages by <t>RT-PCR.</t> c The percentage of F4/80 and NOS2 or CD206 double-positive macrophages was measured by FCM. d The production of TNF-α, IL-10, and TGF-β was measured by ELISA. Data are expressed as the mean ± SEM ( n = 3). * p
Real Time Pcr Assay Total Rna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 8 article reviews
Price from $9.99 to $1999.99
real time pcr assay total rna - by Bioz Stars, 2020-08
90/100 stars

Images

1) Product Images from "Mycobacterium tuberculosis Heat-Shock Protein 16.3 Induces Macrophage M2 Polarization Through CCRL2/CX3CR1"

Article Title: Mycobacterium tuberculosis Heat-Shock Protein 16.3 Induces Macrophage M2 Polarization Through CCRL2/CX3CR1

Journal: Inflammation

doi: 10.1007/s10753-019-01132-9

MTB Hsp16.3 induces mouse bone marrow-derived macrophage (BMDM) M2 polarization. Bone marrow cells were isolated from the tibias and femurs of BALB/c mice (6–8 weeks old) and incubated with 20 ng/ml GM-CSF for 7 days. Then, BMDMs were treated with 100 ng/ml MTB Hsp16.3, 100 ng/ml IFN-γ, or 100 ng/ml IL-4 for 0–72 h. a The morphology of BMDMs incubated with IFN-γ, IL-4, or MTB Hsp16.3 for 12 h. The images were captured under an inverted microscope (× 200); the picture in the upper right corner is × 400. Total RNA was extracted from the cells using TRIzol according to the manufacturer’s instructions. b The mRNA expression levels of iNOS, IL-6, TNF-α, Arg-1, IL-10, and TGF-β in macrophages by RT-PCR. c The percentage of F4/80 and NOS2 or CD206 double-positive macrophages was measured by FCM. d The production of TNF-α, IL-10, and TGF-β was measured by ELISA. Data are expressed as the mean ± SEM ( n = 3). * p
Figure Legend Snippet: MTB Hsp16.3 induces mouse bone marrow-derived macrophage (BMDM) M2 polarization. Bone marrow cells were isolated from the tibias and femurs of BALB/c mice (6–8 weeks old) and incubated with 20 ng/ml GM-CSF for 7 days. Then, BMDMs were treated with 100 ng/ml MTB Hsp16.3, 100 ng/ml IFN-γ, or 100 ng/ml IL-4 for 0–72 h. a The morphology of BMDMs incubated with IFN-γ, IL-4, or MTB Hsp16.3 for 12 h. The images were captured under an inverted microscope (× 200); the picture in the upper right corner is × 400. Total RNA was extracted from the cells using TRIzol according to the manufacturer’s instructions. b The mRNA expression levels of iNOS, IL-6, TNF-α, Arg-1, IL-10, and TGF-β in macrophages by RT-PCR. c The percentage of F4/80 and NOS2 or CD206 double-positive macrophages was measured by FCM. d The production of TNF-α, IL-10, and TGF-β was measured by ELISA. Data are expressed as the mean ± SEM ( n = 3). * p

Techniques Used: Derivative Assay, Isolation, Mouse Assay, Incubation, Inverted Microscopy, Expressing, Reverse Transcription Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

Related Articles

Real-time Polymerase Chain Reaction:

Article Title: Critical role of NLRP3-caspase-1 pathway in age-dependent isoflurane-induced microglial inflammatory response and cognitive impairment
Article Snippet: .. RNA isolation and real-time PCR assay Total RNA was extracted with TRIzol reagent (TaKaRa, Japan). .. Reverse transcription was performed with PrimeScript RT reagent Kit (TaKaRa, Japan) according to the manufacturer’s instructions.

Article Title: A cis‐eQTL genetic variant in PLK4 confers high risk of hepatocellular carcinoma, et al. A cis‐eQTL genetic variant in PLK4 confers high risk of hepatocellular carcinoma
Article Snippet: .. 2.6 Quantitative real time PCR assay Total RNA was extracted by Trizol (Takara Bio, Inc.), and cDNA was synthesized using a reverse transcription kit (Vazyme). .. Quantitative real time PCR was carried out in triplicate with Power SYBR Green Master Mix on an Applied Biosystems QuantStudio 7 Flex Real Time PCR machine.

Article Title: Ozone induces autophagy in rat chondrocytes stimulated with IL-1β through the AMPK/mTOR signaling pathway
Article Snippet: .. Quantitative real-time PCR assay Total RNA was extracted from chondrocytes using Trizol reagent (Takara, Shiga, Japan) in accordance with the manufacturer’s instructions. .. Total RNA (1 µg) was reverse-transcribed into complementary DNA (cDNA) using a PrimeScript RT reagent kit (RR047A; Takara, Shiga, Japan). mRNA expression was measured by quantitative real-time PCR (qRT-PCR) using SYBR® GreenER™ SuperMix (Takara, Shiga, Japan) with specific primers for β-actin: forward 5′-GGAGATTACTGCCCTGGCTCCTA-3′, reverse 5′-GACTCATCGTACTCCTGCTTGCTG-3′, IL-6: forward 5′-ATTGTATGAACAGCGATGATGCAC-3′, reverse 5′-CCAGGTAGAAACGGAACTCCAGA-3′, TNF-α: forward 5′-TCAGTTCCATGGCCCAGAC-3′, reverse 5′-GTTGTCTTTGAGATCCATGCCATT –3′, MMP-13: forward 5′-TGATGATGAAACCTGGACAAGCA-3′, reverse 5′-GAACGTCATCATCTGGGAGCA-3′, and TIMP-1: forward 5′-CATCTCTGGCCTCTGGCATC-3′, reverse 5′-CATAACGCTGGTATAAGGTGGTCTC-3′.

Article Title: Dysregulation of CircRNA_0001946 Contributes to the Proliferation and Metastasis of Colorectal Cancer Cells by Targeting MicroRNA-135a-5p
Article Snippet: .. Quantitative Real-Time PCR Assay Total RNA was extracted with TRIzol reagent (Takara, Dalian, China). .. Then, Prime Script RT Master Mix (Takara) was used to reverse transcribe 500 ng of the extracted RNA into cDNA according to the manufacturer’s protocol.

Article Title: Gambogic acid suppresses hypoxia-induced hypoxia-inducible factor-1α/vascular endothelial growth factor expression via inhibiting phosphatidylinositol 3-kinase/Akt/mammalian target protein of rapamycin pathway in multiple myeloma cells
Article Snippet: .. Quantitative real-time PCR assay Total RNA was isolated from cells by using RNAiso Plus (TaKaRa, Dalian, China). .. Reverse transcription was performed with PrimeScript RT reagent kit with gDNA Eraser (TaKaRa), and real time PCR was carried out using SYBR Premix Ex Taq (TaKaRa).

Article Title: Stathmin is overexpressed and regulated by mutant p53 in oral squamous cell carcinoma
Article Snippet: .. Real-time PCR assay Total RNA from cultured cells was isolated at 80% confluence with TRIzol reagent (Takara, Dalian, China) according to the manufacturer’s instructions. .. Total RNA (1 μg) was reverse transcribed into first strand cDNA with PrimeScript reverse transcriptase (Takara, Dalian, China), random primers and oligo dT primer in a 20 μl reaction mixture according to the manufacturer’s protocol.

Article Title: EriB targeted inhibition of microglia activity attenuates MPP+ induced DA neuron injury through the NF-κB signaling pathway
Article Snippet: .. Real-time PCR assay Total RNA (1 μg) extracted from midbrain of C57BL/6 mice was reverse-transcribed into cDNA using a PrimeScript™ II 1st Strand cDNA Synthesis Kit (TAKARA, Shiga Prefecture, Japan) according to the manufacturer’s instructions. .. Real-time PCR was performed using SYBR® Premix Ex Taq™ (TliRNaseH Plus) (TAKARA, Shiga Prefecture, Japan).

Isolation:

Article Title: Critical role of NLRP3-caspase-1 pathway in age-dependent isoflurane-induced microglial inflammatory response and cognitive impairment
Article Snippet: .. RNA isolation and real-time PCR assay Total RNA was extracted with TRIzol reagent (TaKaRa, Japan). .. Reverse transcription was performed with PrimeScript RT reagent Kit (TaKaRa, Japan) according to the manufacturer’s instructions.

Article Title: Gambogic acid suppresses hypoxia-induced hypoxia-inducible factor-1α/vascular endothelial growth factor expression via inhibiting phosphatidylinositol 3-kinase/Akt/mammalian target protein of rapamycin pathway in multiple myeloma cells
Article Snippet: .. Quantitative real-time PCR assay Total RNA was isolated from cells by using RNAiso Plus (TaKaRa, Dalian, China). .. Reverse transcription was performed with PrimeScript RT reagent kit with gDNA Eraser (TaKaRa), and real time PCR was carried out using SYBR Premix Ex Taq (TaKaRa).

Article Title: Stathmin is overexpressed and regulated by mutant p53 in oral squamous cell carcinoma
Article Snippet: .. Real-time PCR assay Total RNA from cultured cells was isolated at 80% confluence with TRIzol reagent (Takara, Dalian, China) according to the manufacturer’s instructions. .. Total RNA (1 μg) was reverse transcribed into first strand cDNA with PrimeScript reverse transcriptase (Takara, Dalian, China), random primers and oligo dT primer in a 20 μl reaction mixture according to the manufacturer’s protocol.

Synthesized:

Article Title: A cis‐eQTL genetic variant in PLK4 confers high risk of hepatocellular carcinoma, et al. A cis‐eQTL genetic variant in PLK4 confers high risk of hepatocellular carcinoma
Article Snippet: .. 2.6 Quantitative real time PCR assay Total RNA was extracted by Trizol (Takara Bio, Inc.), and cDNA was synthesized using a reverse transcription kit (Vazyme). .. Quantitative real time PCR was carried out in triplicate with Power SYBR Green Master Mix on an Applied Biosystems QuantStudio 7 Flex Real Time PCR machine.

Cell Culture:

Article Title: Stathmin is overexpressed and regulated by mutant p53 in oral squamous cell carcinoma
Article Snippet: .. Real-time PCR assay Total RNA from cultured cells was isolated at 80% confluence with TRIzol reagent (Takara, Dalian, China) according to the manufacturer’s instructions. .. Total RNA (1 μg) was reverse transcribed into first strand cDNA with PrimeScript reverse transcriptase (Takara, Dalian, China), random primers and oligo dT primer in a 20 μl reaction mixture according to the manufacturer’s protocol.

Mouse Assay:

Article Title: EriB targeted inhibition of microglia activity attenuates MPP+ induced DA neuron injury through the NF-κB signaling pathway
Article Snippet: .. Real-time PCR assay Total RNA (1 μg) extracted from midbrain of C57BL/6 mice was reverse-transcribed into cDNA using a PrimeScript™ II 1st Strand cDNA Synthesis Kit (TAKARA, Shiga Prefecture, Japan) according to the manufacturer’s instructions. .. Real-time PCR was performed using SYBR® Premix Ex Taq™ (TliRNaseH Plus) (TAKARA, Shiga Prefecture, Japan).

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    TaKaRa real time quantitative polymerase chain reaction qrt pcr total rna
    LncRNA CASC2 inhibited hypoxia-induced cell proliferation in rat pulmonary arterial tissues. ( a ) The mRNA expression of PCNA was detected by <t>qRT-PCR</t> in rats′ PAs tissues. ( b , c ) The protein expression of PCNA was detected by western blot analysis in rat pulmonary arterial tissues. ## p
    Real Time Quantitative Polymerase Chain Reaction Qrt Pcr Total Rna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time quantitative polymerase chain reaction qrt pcr total rna/product/TaKaRa
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    real time quantitative polymerase chain reaction qrt pcr total rna - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    93
    TaKaRa quantitative real time pcr qrt pcr total rna
    mRNA levels of galectins and the receptors of Gal-9 in the lung and MLN tissues of Pb ANKA-infected mice with or without α-lactose treatment estimated using <t>qRT-PCR.</t> ( a ) mRNA levels of Gal-1, Gal-3, Gal-8, and Gal-9; ( b ) mRNA levels of Tim-3, CD44, CD137, and PDI. Values are means from triplicate measurements, and data are presented as means ± SD; two independent experiments were performed with four mice per group. * P
    Quantitative Real Time Pcr Qrt Pcr Total Rna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 85 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantitative real time pcr qrt pcr total rna/product/TaKaRa
    Average 93 stars, based on 85 article reviews
    Price from $9.99 to $1999.99
    quantitative real time pcr qrt pcr total rna - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

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    LncRNA CASC2 inhibited hypoxia-induced cell proliferation in rat pulmonary arterial tissues. ( a ) The mRNA expression of PCNA was detected by qRT-PCR in rats′ PAs tissues. ( b , c ) The protein expression of PCNA was detected by western blot analysis in rat pulmonary arterial tissues. ## p

    Journal: Respiratory Research

    Article Title: Long non-coding RNA CASC2 suppresses pulmonary artery smooth muscle cell proliferation and phenotypic switch in hypoxia-induced pulmonary hypertension

    doi: 10.1186/s12931-019-1018-x

    Figure Lengend Snippet: LncRNA CASC2 inhibited hypoxia-induced cell proliferation in rat pulmonary arterial tissues. ( a ) The mRNA expression of PCNA was detected by qRT-PCR in rats′ PAs tissues. ( b , c ) The protein expression of PCNA was detected by western blot analysis in rat pulmonary arterial tissues. ## p

    Article Snippet: Real time-quantitative polymerase chain reaction (qRT-PCR) Total RNA from PASMCs or rat pulmonary arterial tissues was extracted using the Trizol reagent and reversely transcribed into cDNA using Prime-Script RT-PCR kit (Takara, Dalian, China) following the manufacturer′s instructions.

    Techniques: Expressing, Quantitative RT-PCR, Western Blot

    Up-regulation of lncRNA CASC2 inhibited proliferation in hypoxia-induced PASMCs. ( a ) The expression of lncRNA CASC2 was detected by qRT-PCR in PASMCs. ( b ) Cell number of PASMCs was counted by CyQuant cell proliferation assay kit. ( c ) The mRNA expression of PCNA was detected by qRT-PCR in PASMCs. ( d ) The protein expression of PCNA was detected by western blot analysis in PASMCs. ( e , f ) Cell proliferation status of PASMCs was assessed by EdU staining assay. ### p

    Journal: Respiratory Research

    Article Title: Long non-coding RNA CASC2 suppresses pulmonary artery smooth muscle cell proliferation and phenotypic switch in hypoxia-induced pulmonary hypertension

    doi: 10.1186/s12931-019-1018-x

    Figure Lengend Snippet: Up-regulation of lncRNA CASC2 inhibited proliferation in hypoxia-induced PASMCs. ( a ) The expression of lncRNA CASC2 was detected by qRT-PCR in PASMCs. ( b ) Cell number of PASMCs was counted by CyQuant cell proliferation assay kit. ( c ) The mRNA expression of PCNA was detected by qRT-PCR in PASMCs. ( d ) The protein expression of PCNA was detected by western blot analysis in PASMCs. ( e , f ) Cell proliferation status of PASMCs was assessed by EdU staining assay. ### p

    Article Snippet: Real time-quantitative polymerase chain reaction (qRT-PCR) Total RNA from PASMCs or rat pulmonary arterial tissues was extracted using the Trizol reagent and reversely transcribed into cDNA using Prime-Script RT-PCR kit (Takara, Dalian, China) following the manufacturer′s instructions.

    Techniques: Expressing, Quantitative RT-PCR, CyQUANT Assay, Proliferation Assay, Western Blot, Staining

    LncRNA CASC2 was down-regulated in hypoxia-induced rats and reversed the increase of mPAP and progression of right ventricular hypertrophy caused by hypoxia. ( a ) The expression of lncRNA CASC2 was detected by qRT-PCR in rat pulmonary arterial tissues. ( b ) Systemic blood pressure (SBP) was assessed in normoxia and hypoxia-induced rats. ( c ) Mean pulmonary arterial pressure (mPAP) was detected in normoxia and hypoxia-induced rats. ( d ) The right ventricular hypertrophy index (RVI) was calculated in normoxia and hypoxia-induced rats. # p

    Journal: Respiratory Research

    Article Title: Long non-coding RNA CASC2 suppresses pulmonary artery smooth muscle cell proliferation and phenotypic switch in hypoxia-induced pulmonary hypertension

    doi: 10.1186/s12931-019-1018-x

    Figure Lengend Snippet: LncRNA CASC2 was down-regulated in hypoxia-induced rats and reversed the increase of mPAP and progression of right ventricular hypertrophy caused by hypoxia. ( a ) The expression of lncRNA CASC2 was detected by qRT-PCR in rat pulmonary arterial tissues. ( b ) Systemic blood pressure (SBP) was assessed in normoxia and hypoxia-induced rats. ( c ) Mean pulmonary arterial pressure (mPAP) was detected in normoxia and hypoxia-induced rats. ( d ) The right ventricular hypertrophy index (RVI) was calculated in normoxia and hypoxia-induced rats. # p

    Article Snippet: Real time-quantitative polymerase chain reaction (qRT-PCR) Total RNA from PASMCs or rat pulmonary arterial tissues was extracted using the Trizol reagent and reversely transcribed into cDNA using Prime-Script RT-PCR kit (Takara, Dalian, China) following the manufacturer′s instructions.

    Techniques: Expressing, Quantitative RT-PCR

    mRNA levels of galectins and the receptors of Gal-9 in the lung and MLN tissues of Pb ANKA-infected mice with or without α-lactose treatment estimated using qRT-PCR. ( a ) mRNA levels of Gal-1, Gal-3, Gal-8, and Gal-9; ( b ) mRNA levels of Tim-3, CD44, CD137, and PDI. Values are means from triplicate measurements, and data are presented as means ± SD; two independent experiments were performed with four mice per group. * P

    Journal: Scientific Reports

    Article Title: Blockage of Galectin-receptor Interactions by α-lactose Exacerbates Plasmodium berghei-induced Pulmonary Immunopathology

    doi: 10.1038/srep32024

    Figure Lengend Snippet: mRNA levels of galectins and the receptors of Gal-9 in the lung and MLN tissues of Pb ANKA-infected mice with or without α-lactose treatment estimated using qRT-PCR. ( a ) mRNA levels of Gal-1, Gal-3, Gal-8, and Gal-9; ( b ) mRNA levels of Tim-3, CD44, CD137, and PDI. Values are means from triplicate measurements, and data are presented as means ± SD; two independent experiments were performed with four mice per group. * P

    Article Snippet: Measurement of mRNA expression using quantitative real-time PCR (qRT-PCR) Total RNA was extracted from about 100 mg of mouse lung and MLN tissues or macrophages of each group using a RNA Extraction Kit (TaKaRa Bio, Inc., Tokyo, Japan) according to the manufacturer’s protocol.

    Techniques: Infection, Mouse Assay, Quantitative RT-PCR

    mRNA levels of IFN-α, IFN-β, IFN-γ, IL-4, and IL-10 in the lung and MLN tissues of Pb ANKA-infected mice with or without α-lactose treatment estimated using qRT-PCR. Values are means from triplicate measurements, and data are presented as means ± SD; two independent experiments were performed with four mice per group. * P

    Journal: Scientific Reports

    Article Title: Blockage of Galectin-receptor Interactions by α-lactose Exacerbates Plasmodium berghei-induced Pulmonary Immunopathology

    doi: 10.1038/srep32024

    Figure Lengend Snippet: mRNA levels of IFN-α, IFN-β, IFN-γ, IL-4, and IL-10 in the lung and MLN tissues of Pb ANKA-infected mice with or without α-lactose treatment estimated using qRT-PCR. Values are means from triplicate measurements, and data are presented as means ± SD; two independent experiments were performed with four mice per group. * P

    Article Snippet: Measurement of mRNA expression using quantitative real-time PCR (qRT-PCR) Total RNA was extracted from about 100 mg of mouse lung and MLN tissues or macrophages of each group using a RNA Extraction Kit (TaKaRa Bio, Inc., Tokyo, Japan) according to the manufacturer’s protocol.

    Techniques: Infection, Mouse Assay, Quantitative RT-PCR

    Comparisons of the protein and mRNA expression patterns of three representative DEPs at four artificial ageing stages (WH98, WH50, WH20, and WH01) by iTRAQ and qRT-PCR. Solid lines represent mRNA expression patterns, and dotted lines represent protein expression patterns.

    Journal: PLoS ONE

    Article Title: Quantitative Proteomic Analysis of Wheat Seeds during Artificial Ageing and Priming Using the Isobaric Tandem Mass Tag Labeling

    doi: 10.1371/journal.pone.0162851

    Figure Lengend Snippet: Comparisons of the protein and mRNA expression patterns of three representative DEPs at four artificial ageing stages (WH98, WH50, WH20, and WH01) by iTRAQ and qRT-PCR. Solid lines represent mRNA expression patterns, and dotted lines represent protein expression patterns.

    Article Snippet: RNA isolation and quantitative real-time PCR (qRT-PCR) analysis Total RNA from wheat embryos of WH98, WH50, WH20, and WH01 were extracted by using RNAiso Plus reagent (Takara, Tokyo, Japan), and genomic DNA was removed by treating with DNase I (Takara) following the manufacturer’s protocol.

    Techniques: Expressing, Quantitative RT-PCR

    The effect of TMPyP4 on cells adhesion to extracellular matrix. ( A–D ) TMPyP4 increase cell adhesion to extracellular matrix. Indicated cell lines were used. ( E ) qRT-PCR showed the abundance of MUC5B mRNA in A549 cancer cell line (NC) and A549 cells transfected with siRNA targeting MUC5B (siRNA_1 and siRNA_2). ( F ) MUC5B knock-down suppress the adhesion of A549 cancer cells to extracellular matrix. MUC5B deficient cells showed no increase of cell adhesion upon TMPyP4 treatment. Values are average ± SD of three independent experiments.

    Journal: Scientific Reports

    Article Title: TMPyP4 promotes cancer cell migration at low doses, but induces cell death at high doses

    doi: 10.1038/srep26592

    Figure Lengend Snippet: The effect of TMPyP4 on cells adhesion to extracellular matrix. ( A–D ) TMPyP4 increase cell adhesion to extracellular matrix. Indicated cell lines were used. ( E ) qRT-PCR showed the abundance of MUC5B mRNA in A549 cancer cell line (NC) and A549 cells transfected with siRNA targeting MUC5B (siRNA_1 and siRNA_2). ( F ) MUC5B knock-down suppress the adhesion of A549 cancer cells to extracellular matrix. MUC5B deficient cells showed no increase of cell adhesion upon TMPyP4 treatment. Values are average ± SD of three independent experiments.

    Article Snippet: Quantitative real-time PCR (qRT-PCR) Total RNA of A549 cells treated with indicated siRNA was extracted by RNAiso Plus Reagent (Takara). cDNA was produced using Superscript III Reverse Transcriptase (Invitrogen) and random primers. qRT-PCR were performed using Fast SYBR Green PCR mastermix (Invitrogen) and specific primers (MUC5B: 5′-GCCTACGAGGACTTCAACGTC-3′, 5′-CCTTGATGACAACACGGGTGA-3′; β-actin: 5′-CATGTACGTTGCTATCCAGGC-3′, 5′-CTCCTTAATGTCACGCACGAT-3′).

    Techniques: Quantitative RT-PCR, Transfection