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Promega rctp
Rctp, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rctp/product/Promega
Average 90 stars, based on 18 article reviews
Price from $9.99 to $1999.99
rctp - by Bioz Stars, 2020-08
90/100 stars

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Related Articles

Amplification:

Article Title: Pentamidine inhibits catalytic activity of group I intron Ca.LSU by altering RNA folding
Article Snippet: .. The PCR amplified DNA fragment containing the SP6 promoter at the 5′ end of the sense strand of a fragment of the rRNA gene containing Ca.LSU was transcribed in vitro in the presence of 500 µM rATP, rCTP and rGTP, 200 µM rUTP (Promega) and 20 µCi [α-32 P]UTP (3000 Ci/mmol; NEN-DuPont), as previously described ( ). ..

In Vitro:

Article Title: Characterization of recombinant Saccharomyces cerevisiae telomerase core enzyme purified from yeast
Article Snippet: .. Tlc1 in vitro transcription was carried out in a 20 μl reaction volume containing 1 μg of DNA template, 2.5 mM each of rATP, rCTP, rTTP and rGTP (Promega), 0.05 unit of pyrophosphatase (Sigma), 40 units of recombinant RNasin, 8 mM GMP (Sigma) and 700 units of T7 RNA polymerase (kindly provided by Professor En-Duo Wang's laboratory in our institute) in transcription buffer (40 mM Tris/HCl, pH 8.0, 5 mM DTT, 30 mM MgCl2 , 1 mM spermidine and 0.1% Triton X-100) for 4 h at 30 °C. .. RNase-free DNase (1 unit) (Promega) was used to remove the DNA template at 37 °C for 30 min, followed by heat-inactivation at 70 °C for 10 min.

Article Title: Pentamidine inhibits catalytic activity of group I intron Ca.LSU by altering RNA folding
Article Snippet: .. The PCR amplified DNA fragment containing the SP6 promoter at the 5′ end of the sense strand of a fragment of the rRNA gene containing Ca.LSU was transcribed in vitro in the presence of 500 µM rATP, rCTP and rGTP, 200 µM rUTP (Promega) and 20 µCi [α-32 P]UTP (3000 Ci/mmol; NEN-DuPont), as previously described ( ). ..

Generated:

Article Title: RNA Directed Modulation of Phenotypic Plasticity in Human Cells
Article Snippet: .. Generating Biotin Labeled RNA Transcripts- Biotin labeled RNA transcripts of Retro-EFI2S2 and GFP were generated by adding the following components into an 8 well PCR strip: (2.5μg of DNA template, 2μl T7 Enzyme mix (Sigma), 1ul rATP, rGTP, rCTP, rUTP (Promega), 1μl Biotin-14-CTP (Life Technologies), 2.5μl 1M DTT (Sigma) and 1μl RNase Inhibitor (Life Technologies)(as described in [ ]). .. The reaction volume was made up to 50μl with RNase free water (QIAGEN).

Labeling:

Article Title: RNA Directed Modulation of Phenotypic Plasticity in Human Cells
Article Snippet: .. Generating Biotin Labeled RNA Transcripts- Biotin labeled RNA transcripts of Retro-EFI2S2 and GFP were generated by adding the following components into an 8 well PCR strip: (2.5μg of DNA template, 2μl T7 Enzyme mix (Sigma), 1ul rATP, rGTP, rCTP, rUTP (Promega), 1μl Biotin-14-CTP (Life Technologies), 2.5μl 1M DTT (Sigma) and 1μl RNase Inhibitor (Life Technologies)(as described in [ ]). .. The reaction volume was made up to 50μl with RNase free water (QIAGEN).

Incubation:

Article Title: Long-Term Follow-Up of Chimpanzees Inoculated with the First Infectious Clone for Hepatitis C Virus
Article Snippet: .. The 100-μl reaction mixture, containing 20 μl of 5× T7 buffer; 7.5 mM (each) rATP, rCTP, rGTP, and rUTP; 2 μg of DNA; and 10 μl of enzyme mixture was incubated at 37°C for 2 h and then treated with 10 U of RNase-free DNase (Promega) for 30 min at 37°C. ..

Stripping Membranes:

Article Title: RNA Directed Modulation of Phenotypic Plasticity in Human Cells
Article Snippet: .. Generating Biotin Labeled RNA Transcripts- Biotin labeled RNA transcripts of Retro-EFI2S2 and GFP were generated by adding the following components into an 8 well PCR strip: (2.5μg of DNA template, 2μl T7 Enzyme mix (Sigma), 1ul rATP, rGTP, rCTP, rUTP (Promega), 1μl Biotin-14-CTP (Life Technologies), 2.5μl 1M DTT (Sigma) and 1μl RNase Inhibitor (Life Technologies)(as described in [ ]). .. The reaction volume was made up to 50μl with RNase free water (QIAGEN).

Polymerase Chain Reaction:

Article Title: RNA Directed Modulation of Phenotypic Plasticity in Human Cells
Article Snippet: .. Generating Biotin Labeled RNA Transcripts- Biotin labeled RNA transcripts of Retro-EFI2S2 and GFP were generated by adding the following components into an 8 well PCR strip: (2.5μg of DNA template, 2μl T7 Enzyme mix (Sigma), 1ul rATP, rGTP, rCTP, rUTP (Promega), 1μl Biotin-14-CTP (Life Technologies), 2.5μl 1M DTT (Sigma) and 1μl RNase Inhibitor (Life Technologies)(as described in [ ]). .. The reaction volume was made up to 50μl with RNase free water (QIAGEN).

Article Title: Defining a novel leptin–melanocortin–kisspeptin pathway involved in the metabolic control of puberty
Article Snippet: .. For the generation of the antisense Kiss1 riboprobe, the product of the PCR was used as template for the transcription reaction, as follows (final volume of 20 μl): 250 Ci [33P]-UTP (Perkin Elmer, Massachusetts, USA), 0.5 μg of template, 2 μl 3NTPs (5 mM rATP, rCTP and rGTP), 1 μl RNasin Ribonuclease Inhibitor (Promega), 4 μl transcription buffer, and 2 μl T7 RNA polymerase (Promega). .. After 120 min of incubation at 37 °C, another 1 μl of T7 RNA was added to the mix, and the reaction was maintained for an additional 60 min at 37 °C.

Article Title: Pentamidine inhibits catalytic activity of group I intron Ca.LSU by altering RNA folding
Article Snippet: .. The PCR amplified DNA fragment containing the SP6 promoter at the 5′ end of the sense strand of a fragment of the rRNA gene containing Ca.LSU was transcribed in vitro in the presence of 500 µM rATP, rCTP and rGTP, 200 µM rUTP (Promega) and 20 µCi [α-32 P]UTP (3000 Ci/mmol; NEN-DuPont), as previously described ( ). ..

Recombinant:

Article Title: Characterization of recombinant Saccharomyces cerevisiae telomerase core enzyme purified from yeast
Article Snippet: .. Tlc1 in vitro transcription was carried out in a 20 μl reaction volume containing 1 μg of DNA template, 2.5 mM each of rATP, rCTP, rTTP and rGTP (Promega), 0.05 unit of pyrophosphatase (Sigma), 40 units of recombinant RNasin, 8 mM GMP (Sigma) and 700 units of T7 RNA polymerase (kindly provided by Professor En-Duo Wang's laboratory in our institute) in transcription buffer (40 mM Tris/HCl, pH 8.0, 5 mM DTT, 30 mM MgCl2 , 1 mM spermidine and 0.1% Triton X-100) for 4 h at 30 °C. .. RNase-free DNase (1 unit) (Promega) was used to remove the DNA template at 37 °C for 30 min, followed by heat-inactivation at 70 °C for 10 min.

Plasmid Preparation:

Article Title: Stem-Loop IV in the 5? Untranslated Region Is a cis-Acting Element in Bovine Coronavirus Defective Interfering RNA Replication
Article Snippet: .. For synthesis of uniformly radiolabeled RNA probe, plasmid DNA of wild-type (wt) pSLIV(PB) or the PB modifications of stem-loop IV mutants (Table ) was linearized with NcoI, and 2.5 μg was transcribed in a 50-μl reaction volume with 40 U T7 RNA polymerase (Promega) at 37°C for 1 h in the presence of 120 μCi [α-32 P]UTP (300 Ci/mmole; ICN), 0.5 mM each of rATP, rCTP, and rGTP (Promega), 12 μM UTP (Promega), 10 mM DTT, and 5 mg acetylated bovine serum albumin (Promega). .. Radiolabeled RNA was treated with 2.5 U RNase-free DNase (Promega) at 37°C for 30 min, phenol-chloroform extracted, electrophoretically resolved on an 8 M urea-6% polyacrylamide gel after the addition of 50 μl loading dye (95% formamide, 20 mM EDTA, 0.3% bromophenol blue and xylene cyanol [wt/vol]).

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  • 90
    Promega rctp
    Rctp, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rctp/product/Promega
    Average 90 stars, based on 18 article reviews
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    Promega α 32 p rctp
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    Promega α 32 p rctp labeled pre mrnas
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