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U73122 PLC rbl 2h3 cells
Measurement of the [Ca 2+ ] C and calcein leakage after TiO 2 NP exposure . (A) <t>RBL-2H3</t> cells were stimulated with TiO 2 NPs with concentrations of 0.1 mg/ml (orange), 0.25 mg/ml (green), 0.5 mg/ml (purple), 0.75 mg/ml (blue) and 1 mg/ml (red) in normal Hanks' solution, (B) in Ca 2+ -free Hanks' solution, (C) in the presence of CdCl 2 (200 μM), (D) nifedipine (10 μM), (E) verapamil (100 μM), and (F) calcein (50 μM) (n ≥ 12, **p
Rbl 2h3 Cells, supplied by U73122 PLC, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "A mixture of anatase and rutile TiO2 nanoparticles induces histamine secretion in mast cells"

Article Title: A mixture of anatase and rutile TiO2 nanoparticles induces histamine secretion in mast cells

Journal: Particle and Fibre Toxicology

doi: 10.1186/1743-8977-9-2

Measurement of the [Ca 2+ ] C and calcein leakage after TiO 2 NP exposure . (A) RBL-2H3 cells were stimulated with TiO 2 NPs with concentrations of 0.1 mg/ml (orange), 0.25 mg/ml (green), 0.5 mg/ml (purple), 0.75 mg/ml (blue) and 1 mg/ml (red) in normal Hanks' solution, (B) in Ca 2+ -free Hanks' solution, (C) in the presence of CdCl 2 (200 μM), (D) nifedipine (10 μM), (E) verapamil (100 μM), and (F) calcein (50 μM) (n ≥ 12, **p
Figure Legend Snippet: Measurement of the [Ca 2+ ] C and calcein leakage after TiO 2 NP exposure . (A) RBL-2H3 cells were stimulated with TiO 2 NPs with concentrations of 0.1 mg/ml (orange), 0.25 mg/ml (green), 0.5 mg/ml (purple), 0.75 mg/ml (blue) and 1 mg/ml (red) in normal Hanks' solution, (B) in Ca 2+ -free Hanks' solution, (C) in the presence of CdCl 2 (200 μM), (D) nifedipine (10 μM), (E) verapamil (100 μM), and (F) calcein (50 μM) (n ≥ 12, **p

Techniques Used:

Measurement of histamine secretion evoked by TiO 2 NPs . (A) ELISA quantification of histamine release from RBL-2H3 cells after TiO 2 NP (0.1 mg/ml-0.75 mg/ml stimulation) in normal Hanks' solution (n ≥ 3, **p
Figure Legend Snippet: Measurement of histamine secretion evoked by TiO 2 NPs . (A) ELISA quantification of histamine release from RBL-2H3 cells after TiO 2 NP (0.1 mg/ml-0.75 mg/ml stimulation) in normal Hanks' solution (n ≥ 3, **p

Techniques Used: Enzyme-linked Immunosorbent Assay

Measurement of intracellular ROS level and the associated changes in [Ca 2+ ] C . (A) RBL-2H3 cells were exposed to TiO 2 NPs at concentrations 0, 0.1, 0.25, 0.5 and 0.75 mg/ml. The generation of ROS was measured by fluorescence imaging. The level of ROS increased as a function of increasing TiO 2 NP concentration (n ≥ 50, **p
Figure Legend Snippet: Measurement of intracellular ROS level and the associated changes in [Ca 2+ ] C . (A) RBL-2H3 cells were exposed to TiO 2 NPs at concentrations 0, 0.1, 0.25, 0.5 and 0.75 mg/ml. The generation of ROS was measured by fluorescence imaging. The level of ROS increased as a function of increasing TiO 2 NP concentration (n ≥ 50, **p

Techniques Used: Fluorescence, Imaging, Concentration Assay

Measurement of [Ca 2+ ] C after stimulation by TiO 2 NPs . RBL-2H3 cells were stimulated with TiO 2 NPs with concentrations that vary from 0.1 mg/ml-1 mg/ml, in the presence of (A) U73122 (10 μM), (B) 2-APB (50 μM) and (C) Xestospongin (20 μM) and (D) Thapsigargin (100 nM). The colors used are in accordance with Figure 2A. Each line represents the average fluorescent intensity of more than 200 cells.
Figure Legend Snippet: Measurement of [Ca 2+ ] C after stimulation by TiO 2 NPs . RBL-2H3 cells were stimulated with TiO 2 NPs with concentrations that vary from 0.1 mg/ml-1 mg/ml, in the presence of (A) U73122 (10 μM), (B) 2-APB (50 μM) and (C) Xestospongin (20 μM) and (D) Thapsigargin (100 nM). The colors used are in accordance with Figure 2A. Each line represents the average fluorescent intensity of more than 200 cells.

Techniques Used:

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Article Title: A mixture of anatase and rutile TiO2 nanoparticles induces histamine secretion in mast cells
Article Snippet: .. The IP3 -IP3 receptor as a histamine-release pathway To understand the influence of PLC, and the ensuing IP3 -IP3 receptor pathway on cytosolic Ca2+ , the RBL-2H3 cells were pre-treated with U73122 (PLC inhibitor), 2-APB or Xestospongin (IP3 receptor blockers), before being exposed to TiO2 NPs. ..

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    U73122 PLC rbl 2h3 cells
    Measurement of the [Ca 2+ ] C and calcein leakage after TiO 2 NP exposure . (A) <t>RBL-2H3</t> cells were stimulated with TiO 2 NPs with concentrations of 0.1 mg/ml (orange), 0.25 mg/ml (green), 0.5 mg/ml (purple), 0.75 mg/ml (blue) and 1 mg/ml (red) in normal Hanks' solution, (B) in Ca 2+ -free Hanks' solution, (C) in the presence of CdCl 2 (200 μM), (D) nifedipine (10 μM), (E) verapamil (100 μM), and (F) calcein (50 μM) (n ≥ 12, **p
    Rbl 2h3 Cells, supplied by U73122 PLC, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rbl 2h3 cells/product/U73122 PLC
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    rbl 2h3 cells - by Bioz Stars, 2020-11
    92/100 stars
      Buy from Supplier

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    Measurement of the [Ca 2+ ] C and calcein leakage after TiO 2 NP exposure . (A) RBL-2H3 cells were stimulated with TiO 2 NPs with concentrations of 0.1 mg/ml (orange), 0.25 mg/ml (green), 0.5 mg/ml (purple), 0.75 mg/ml (blue) and 1 mg/ml (red) in normal Hanks' solution, (B) in Ca 2+ -free Hanks' solution, (C) in the presence of CdCl 2 (200 μM), (D) nifedipine (10 μM), (E) verapamil (100 μM), and (F) calcein (50 μM) (n ≥ 12, **p

    Journal: Particle and Fibre Toxicology

    Article Title: A mixture of anatase and rutile TiO2 nanoparticles induces histamine secretion in mast cells

    doi: 10.1186/1743-8977-9-2

    Figure Lengend Snippet: Measurement of the [Ca 2+ ] C and calcein leakage after TiO 2 NP exposure . (A) RBL-2H3 cells were stimulated with TiO 2 NPs with concentrations of 0.1 mg/ml (orange), 0.25 mg/ml (green), 0.5 mg/ml (purple), 0.75 mg/ml (blue) and 1 mg/ml (red) in normal Hanks' solution, (B) in Ca 2+ -free Hanks' solution, (C) in the presence of CdCl 2 (200 μM), (D) nifedipine (10 μM), (E) verapamil (100 μM), and (F) calcein (50 μM) (n ≥ 12, **p

    Article Snippet: The IP3 -IP3 receptor as a histamine-release pathway To understand the influence of PLC, and the ensuing IP3 -IP3 receptor pathway on cytosolic Ca2+ , the RBL-2H3 cells were pre-treated with U73122 (PLC inhibitor), 2-APB or Xestospongin (IP3 receptor blockers), before being exposed to TiO2 NPs.

    Techniques:

    Measurement of histamine secretion evoked by TiO 2 NPs . (A) ELISA quantification of histamine release from RBL-2H3 cells after TiO 2 NP (0.1 mg/ml-0.75 mg/ml stimulation) in normal Hanks' solution (n ≥ 3, **p

    Journal: Particle and Fibre Toxicology

    Article Title: A mixture of anatase and rutile TiO2 nanoparticles induces histamine secretion in mast cells

    doi: 10.1186/1743-8977-9-2

    Figure Lengend Snippet: Measurement of histamine secretion evoked by TiO 2 NPs . (A) ELISA quantification of histamine release from RBL-2H3 cells after TiO 2 NP (0.1 mg/ml-0.75 mg/ml stimulation) in normal Hanks' solution (n ≥ 3, **p

    Article Snippet: The IP3 -IP3 receptor as a histamine-release pathway To understand the influence of PLC, and the ensuing IP3 -IP3 receptor pathway on cytosolic Ca2+ , the RBL-2H3 cells were pre-treated with U73122 (PLC inhibitor), 2-APB or Xestospongin (IP3 receptor blockers), before being exposed to TiO2 NPs.

    Techniques: Enzyme-linked Immunosorbent Assay

    Measurement of intracellular ROS level and the associated changes in [Ca 2+ ] C . (A) RBL-2H3 cells were exposed to TiO 2 NPs at concentrations 0, 0.1, 0.25, 0.5 and 0.75 mg/ml. The generation of ROS was measured by fluorescence imaging. The level of ROS increased as a function of increasing TiO 2 NP concentration (n ≥ 50, **p

    Journal: Particle and Fibre Toxicology

    Article Title: A mixture of anatase and rutile TiO2 nanoparticles induces histamine secretion in mast cells

    doi: 10.1186/1743-8977-9-2

    Figure Lengend Snippet: Measurement of intracellular ROS level and the associated changes in [Ca 2+ ] C . (A) RBL-2H3 cells were exposed to TiO 2 NPs at concentrations 0, 0.1, 0.25, 0.5 and 0.75 mg/ml. The generation of ROS was measured by fluorescence imaging. The level of ROS increased as a function of increasing TiO 2 NP concentration (n ≥ 50, **p

    Article Snippet: The IP3 -IP3 receptor as a histamine-release pathway To understand the influence of PLC, and the ensuing IP3 -IP3 receptor pathway on cytosolic Ca2+ , the RBL-2H3 cells were pre-treated with U73122 (PLC inhibitor), 2-APB or Xestospongin (IP3 receptor blockers), before being exposed to TiO2 NPs.

    Techniques: Fluorescence, Imaging, Concentration Assay

    Measurement of [Ca 2+ ] C after stimulation by TiO 2 NPs . RBL-2H3 cells were stimulated with TiO 2 NPs with concentrations that vary from 0.1 mg/ml-1 mg/ml, in the presence of (A) U73122 (10 μM), (B) 2-APB (50 μM) and (C) Xestospongin (20 μM) and (D) Thapsigargin (100 nM). The colors used are in accordance with Figure 2A. Each line represents the average fluorescent intensity of more than 200 cells.

    Journal: Particle and Fibre Toxicology

    Article Title: A mixture of anatase and rutile TiO2 nanoparticles induces histamine secretion in mast cells

    doi: 10.1186/1743-8977-9-2

    Figure Lengend Snippet: Measurement of [Ca 2+ ] C after stimulation by TiO 2 NPs . RBL-2H3 cells were stimulated with TiO 2 NPs with concentrations that vary from 0.1 mg/ml-1 mg/ml, in the presence of (A) U73122 (10 μM), (B) 2-APB (50 μM) and (C) Xestospongin (20 μM) and (D) Thapsigargin (100 nM). The colors used are in accordance with Figure 2A. Each line represents the average fluorescent intensity of more than 200 cells.

    Article Snippet: The IP3 -IP3 receptor as a histamine-release pathway To understand the influence of PLC, and the ensuing IP3 -IP3 receptor pathway on cytosolic Ca2+ , the RBL-2H3 cells were pre-treated with U73122 (PLC inhibitor), 2-APB or Xestospongin (IP3 receptor blockers), before being exposed to TiO2 NPs.

    Techniques: