antibodies anti rbd  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc antibodies anti rbd
    Antibodies Anti Rbd, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    sars cov 2 spike rbd ace2 blocking antibody detection elisa kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc sars cov 2 spike rbd ace2 blocking antibody detection elisa kit
    Sars Cov 2 Spike Rbd Ace2 Blocking Antibody Detection Elisa Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rbd  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rbd
    Rbd, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti rbd e2t6m  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti rbd e2t6m
    Anti Rbd E2t6m, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    raf rbd  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc raf rbd
    Raf Rbd, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    raf rbd  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc raf rbd
    Raf Rbd, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    sars cov 2 spike rbd ace2 blocking antibody detection elisa kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc sars cov 2 spike rbd ace2 blocking antibody detection elisa kit
    Schematic representation of ( A ) <t>SARS-CoV-2</t> S pseudotyped lentivirus infection aided by S protein <t>ACE2</t> binding and TMPRSS2. Knocking down of ACE2 and TMPRSS2 using LNP-siRNA LNP-trim inhibiting lentivirus entry ( B ). The presence of LNPs with surface ACE2 peptide (LNP-trap1), rhACE2 (LNP-trap2), and mAb (LNP-trap3) bind to the lentivirus, thereby inhibiting cell entry and infection ( C ).
    Sars Cov 2 Spike Rbd Ace2 Blocking Antibody Detection Elisa Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sars cov 2 spike rbd ace2 blocking antibody detection elisa kit/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sars cov 2 spike rbd ace2 blocking antibody detection elisa kit - by Bioz Stars, 2024-07
    86/100 stars

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    1) Product Images from "Lipid Nanoparticle-Based Inhibitors for SARS-CoV-2 Host Cell Infection"

    Article Title: Lipid Nanoparticle-Based Inhibitors for SARS-CoV-2 Host Cell Infection

    Journal: International Journal of Nanomedicine

    doi: 10.2147/IJN.S448005

    Schematic representation of ( A ) SARS-CoV-2 S pseudotyped lentivirus infection aided by S protein ACE2 binding and TMPRSS2. Knocking down of ACE2 and TMPRSS2 using LNP-siRNA LNP-trim inhibiting lentivirus entry ( B ). The presence of LNPs with surface ACE2 peptide (LNP-trap1), rhACE2 (LNP-trap2), and mAb (LNP-trap3) bind to the lentivirus, thereby inhibiting cell entry and infection ( C ).
    Figure Legend Snippet: Schematic representation of ( A ) SARS-CoV-2 S pseudotyped lentivirus infection aided by S protein ACE2 binding and TMPRSS2. Knocking down of ACE2 and TMPRSS2 using LNP-siRNA LNP-trim inhibiting lentivirus entry ( B ). The presence of LNPs with surface ACE2 peptide (LNP-trap1), rhACE2 (LNP-trap2), and mAb (LNP-trap3) bind to the lentivirus, thereby inhibiting cell entry and infection ( C ).

    Techniques Used: Infection, Binding Assay

    Cytotoxicity of LNP-COOH analyzed using ( A ) cell impedance and ( B ) MTT assays in Calu-3 cells. Cytotoxicity MTT assays of LNP-PEP ( C ), LNP-rhACE2 ( D ), LNP-mAb ( E ), and LNP-si ACE2 ( F ) in Calu-3 cells. Values are expressed as the mean ± standard error of the mean (n = 3).
    Figure Legend Snippet: Cytotoxicity of LNP-COOH analyzed using ( A ) cell impedance and ( B ) MTT assays in Calu-3 cells. Cytotoxicity MTT assays of LNP-PEP ( C ), LNP-rhACE2 ( D ), LNP-mAb ( E ), and LNP-si ACE2 ( F ) in Calu-3 cells. Values are expressed as the mean ± standard error of the mean (n = 3).

    Techniques Used:

    Quantitative determination of cellular accumulation of ( A ) LNP-PEP and ( B ) LNP-rhACE2 in Calu-3 cells over time. Representative confocal microscopy images from Calu-3 cells treated (3 hours) with cell-impermeable LNP-PEP ( C ‒ F ) and cell-permeable LNP- si ACE2 ( G ‒ J ). Plasma membrane from Calu-3 cells was stained with WGA Alexa Fluor TM 594 ( C and G ); LNP localization in Calu-3 cells was visualized with green fluorescent DiO dye ( D and H ); and cell nuclei were stained using Hoestch ( E and I ). Composite overlay of all three images showing cell membrane, nuclei, and LNPs ( F and J ). Orthogonal projections of Calu-3 cells treated with LNP-si ACE2 ( K ) for 3 hours. Two-dimensional images in z-position where red lines indicate cross-section through y-position and green lines indicate cross-section through x-position. The white dashed box in image K is enlarged. Values are expressed as the mean ± standard error of the mean (n = 3).
    Figure Legend Snippet: Quantitative determination of cellular accumulation of ( A ) LNP-PEP and ( B ) LNP-rhACE2 in Calu-3 cells over time. Representative confocal microscopy images from Calu-3 cells treated (3 hours) with cell-impermeable LNP-PEP ( C ‒ F ) and cell-permeable LNP- si ACE2 ( G ‒ J ). Plasma membrane from Calu-3 cells was stained with WGA Alexa Fluor TM 594 ( C and G ); LNP localization in Calu-3 cells was visualized with green fluorescent DiO dye ( D and H ); and cell nuclei were stained using Hoestch ( E and I ). Composite overlay of all three images showing cell membrane, nuclei, and LNPs ( F and J ). Orthogonal projections of Calu-3 cells treated with LNP-si ACE2 ( K ) for 3 hours. Two-dimensional images in z-position where red lines indicate cross-section through y-position and green lines indicate cross-section through x-position. The white dashed box in image K is enlarged. Values are expressed as the mean ± standard error of the mean (n = 3).

    Techniques Used: Confocal Microscopy, Membrane, Staining

    Relative ACE2 ( A ) and TMPRSS2( B ) mRNA levels in HEK-293-hACE2 cells at different time points after transfection with LNP-si ACE2 (40 nM si ACE2 ) and LNP-si TMPRSS2 (40 nM si TMPRSS2 ), respectively. The control cells in ( A ) and ( B ) received LNP-siSCR. Relative ACE2 mRNA level in Calu-3 cells 72 hours after receiving LNP-si ACE2 (40 nM si ACE2 ) against control cells that received LNP-siSCR ( C ). Relative ACE2 protein level in Calu-3 cells 72 hours after receiving LNP-si ACE2 (40 nM si ACE2 ) against control cells that received LNP-siSCR ( D ). The uncropped blots are shown in . Values are expressed as the mean ± standard error of the mean (n = 3). Statistical significance between the control and treatment groups were analyzed using t -test (**p < 0.01, ***p<0.001, ****p<0.0001; p < 0.05 was considered statistically significant).
    Figure Legend Snippet: Relative ACE2 ( A ) and TMPRSS2( B ) mRNA levels in HEK-293-hACE2 cells at different time points after transfection with LNP-si ACE2 (40 nM si ACE2 ) and LNP-si TMPRSS2 (40 nM si TMPRSS2 ), respectively. The control cells in ( A ) and ( B ) received LNP-siSCR. Relative ACE2 mRNA level in Calu-3 cells 72 hours after receiving LNP-si ACE2 (40 nM si ACE2 ) against control cells that received LNP-siSCR ( C ). Relative ACE2 protein level in Calu-3 cells 72 hours after receiving LNP-si ACE2 (40 nM si ACE2 ) against control cells that received LNP-siSCR ( D ). The uncropped blots are shown in . Values are expressed as the mean ± standard error of the mean (n = 3). Statistical significance between the control and treatment groups were analyzed using t -test (**p < 0.01, ***p<0.001, ****p<0.0001; p < 0.05 was considered statistically significant).

    Techniques Used: Transfection

    Transduction efficiency (luciferase expression) of SARS-CoV-2 S pseudotyped luciferase lentivirus at the given MOIs in HEK-293 and HEK-293-hACE2 cell lines ( A ). Percentage inhibition of infection in ACE2 KD and TMPRSS2 KD HEK-293-hACE2 cells ( B ). Percentage inhibition of infection in ACE2 KD ( C ) and TMPRSS2 KD ( D ) in airway epithelial Calu-3 cell line at different siRNA concentrations (LNP-siRNA: 1, 2, 10, 20 and 40 nM; presented in the graph as log 10 values). The nonlinear regression curve fit analysis of siRNA concentration vs inhibition is presented in the graphs. Values are expressed as the mean ± standard error of the mean (n =3). Statistical analysis was performed using one-way ANOVA, followed by Tukey’s test (*p < 0.05, **p<0.01; p < 0.05 was considered statistically significant).
    Figure Legend Snippet: Transduction efficiency (luciferase expression) of SARS-CoV-2 S pseudotyped luciferase lentivirus at the given MOIs in HEK-293 and HEK-293-hACE2 cell lines ( A ). Percentage inhibition of infection in ACE2 KD and TMPRSS2 KD HEK-293-hACE2 cells ( B ). Percentage inhibition of infection in ACE2 KD ( C ) and TMPRSS2 KD ( D ) in airway epithelial Calu-3 cell line at different siRNA concentrations (LNP-siRNA: 1, 2, 10, 20 and 40 nM; presented in the graph as log 10 values). The nonlinear regression curve fit analysis of siRNA concentration vs inhibition is presented in the graphs. Values are expressed as the mean ± standard error of the mean (n =3). Statistical analysis was performed using one-way ANOVA, followed by Tukey’s test (*p < 0.05, **p<0.01; p < 0.05 was considered statistically significant).

    Techniques Used: Transduction, Luciferase, Expressing, Inhibition, Infection, Concentration Assay

    Percentage inhibition of SARS-CoV-2 S pseudotyped luciferase lentivirus (MOI = 2) infection in Calu-3 cells in the presence of different concentrations (Log ( C ) of ACE2 peptide and LNP-PEP ( A ), rhACE2 and LNP-rhACE2 ( B ), mAb and LNP-mAb ( C ). The nonlinear regression curve fit analysis of concentration vs inhibition is presented in the graphs. Values are expressed as the mean ± standard error of the mean (n = 3).
    Figure Legend Snippet: Percentage inhibition of SARS-CoV-2 S pseudotyped luciferase lentivirus (MOI = 2) infection in Calu-3 cells in the presence of different concentrations (Log ( C ) of ACE2 peptide and LNP-PEP ( A ), rhACE2 and LNP-rhACE2 ( B ), mAb and LNP-mAb ( C ). The nonlinear regression curve fit analysis of concentration vs inhibition is presented in the graphs. Values are expressed as the mean ± standard error of the mean (n = 3).

    Techniques Used: Inhibition, Luciferase, Infection, Concentration Assay

    Percentage inhibition of SARS-Cov-2 S pseudotyped luciferase lentivirus infection (MOI = 2) in airway epithelial Calu-3 cell line following a combination of conditions including LNP-PEP treatment and ACE2 and TMPRSS2 knockdown. The extent of infection was measured following a luciferase assay. Values are expressed as the mean ± standard error of the mean (n = 3). Statistical analysis was performed using one-way ANOVA, followed by Tukey’s test (*p < 0.05, **p < 0.01, ***p<0.001, ****p<0.0001; p < 0.05 was considered statistically significant).
    Figure Legend Snippet: Percentage inhibition of SARS-Cov-2 S pseudotyped luciferase lentivirus infection (MOI = 2) in airway epithelial Calu-3 cell line following a combination of conditions including LNP-PEP treatment and ACE2 and TMPRSS2 knockdown. The extent of infection was measured following a luciferase assay. Values are expressed as the mean ± standard error of the mean (n = 3). Statistical analysis was performed using one-way ANOVA, followed by Tukey’s test (*p < 0.05, **p < 0.01, ***p<0.001, ****p<0.0001; p < 0.05 was considered statistically significant).

    Techniques Used: Inhibition, Luciferase, Infection

    Percentage inhibition of SARS-Cov-2 S pseudotyped luciferase LV infection (MOI = 2) in airway epithelial Calu-3 cell line following treatment with different concentrations of lactoferrin ( A ), camostat mesylate ( B ), and carrageenan ( C ). Percentage inhibition of infection in Calu-3 cells with various combinatory treatments of LNP-PEP (0.1 µg/mL), lactoferrin (10 µM), camostat mesylate (1 µM), and carrageenan (1 µM) ( D ). The extent of infection was measured following a luciferase assay. Values are expressed as the mean ± standard error of the mean (n = 3). Statistical analysis was performed using one-way ANOVA, followed by Tukey’s test (*p < 0.05, ****p<0.0001; p < 0.05 was considered statistically significant).
    Figure Legend Snippet: Percentage inhibition of SARS-Cov-2 S pseudotyped luciferase LV infection (MOI = 2) in airway epithelial Calu-3 cell line following treatment with different concentrations of lactoferrin ( A ), camostat mesylate ( B ), and carrageenan ( C ). Percentage inhibition of infection in Calu-3 cells with various combinatory treatments of LNP-PEP (0.1 µg/mL), lactoferrin (10 µM), camostat mesylate (1 µM), and carrageenan (1 µM) ( D ). The extent of infection was measured following a luciferase assay. Values are expressed as the mean ± standard error of the mean (n = 3). Statistical analysis was performed using one-way ANOVA, followed by Tukey’s test (*p < 0.05, ****p<0.0001; p < 0.05 was considered statistically significant).

    Techniques Used: Inhibition, Luciferase, Infection

    Distribution of LNP-PEP ( A ) and LNP-si ACE2 ( B ) at different time points up to 24 hours after intranasal administration in nude mice. The LNPs were tagged with IR dye (DiR) to aid visualization. The NIR fluorescence images (Ex/Em 745/800) were acquired and analyzed using IVIS imager. The quantitative analysis of fluorescent LNP-PEP in the head ( C ) and body ( D ) regions at different time points. The quantitative analysis of fluorescent LNP-si ACE2 in the head ( E ) and body ( F ) regions at different time points. Values are expressed as the mean ± standard error of the mean (n = 4).
    Figure Legend Snippet: Distribution of LNP-PEP ( A ) and LNP-si ACE2 ( B ) at different time points up to 24 hours after intranasal administration in nude mice. The LNPs were tagged with IR dye (DiR) to aid visualization. The NIR fluorescence images (Ex/Em 745/800) were acquired and analyzed using IVIS imager. The quantitative analysis of fluorescent LNP-PEP in the head ( C ) and body ( D ) regions at different time points. The quantitative analysis of fluorescent LNP-si ACE2 in the head ( E ) and body ( F ) regions at different time points. Values are expressed as the mean ± standard error of the mean (n = 4).

    Techniques Used: Fluorescence

    sars cov 2 spike rbd ace2 blocking antibody detection elisa kit  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc sars cov 2 spike rbd ace2 blocking antibody detection elisa kit
    Schematic representation of ( A ) <t>SARS-CoV-2</t> S pseudotyped lentivirus infection aided by S protein <t>ACE2</t> binding and TMPRSS2. Knocking down of ACE2 and TMPRSS2 using LNP-siRNA LNP-trim inhibiting lentivirus entry ( B ). The presence of LNPs with surface ACE2 peptide (LNP-trap1), rhACE2 (LNP-trap2), and mAb (LNP-trap3) bind to the lentivirus, thereby inhibiting cell entry and infection ( C ).
    Sars Cov 2 Spike Rbd Ace2 Blocking Antibody Detection Elisa Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sars cov 2 spike rbd ace2 blocking antibody detection elisa kit/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sars cov 2 spike rbd ace2 blocking antibody detection elisa kit - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "Lipid Nanoparticle-Based Inhibitors for SARS-CoV-2 Host Cell Infection"

    Article Title: Lipid Nanoparticle-Based Inhibitors for SARS-CoV-2 Host Cell Infection

    Journal: International Journal of Nanomedicine

    doi: 10.2147/IJN.S448005

    Schematic representation of ( A ) SARS-CoV-2 S pseudotyped lentivirus infection aided by S protein ACE2 binding and TMPRSS2. Knocking down of ACE2 and TMPRSS2 using LNP-siRNA LNP-trim inhibiting lentivirus entry ( B ). The presence of LNPs with surface ACE2 peptide (LNP-trap1), rhACE2 (LNP-trap2), and mAb (LNP-trap3) bind to the lentivirus, thereby inhibiting cell entry and infection ( C ).
    Figure Legend Snippet: Schematic representation of ( A ) SARS-CoV-2 S pseudotyped lentivirus infection aided by S protein ACE2 binding and TMPRSS2. Knocking down of ACE2 and TMPRSS2 using LNP-siRNA LNP-trim inhibiting lentivirus entry ( B ). The presence of LNPs with surface ACE2 peptide (LNP-trap1), rhACE2 (LNP-trap2), and mAb (LNP-trap3) bind to the lentivirus, thereby inhibiting cell entry and infection ( C ).

    Techniques Used: Infection, Binding Assay

    Cytotoxicity of LNP-COOH analyzed using ( A ) cell impedance and ( B ) MTT assays in Calu-3 cells. Cytotoxicity MTT assays of LNP-PEP ( C ), LNP-rhACE2 ( D ), LNP-mAb ( E ), and LNP-si ACE2 ( F ) in Calu-3 cells. Values are expressed as the mean ± standard error of the mean (n = 3).
    Figure Legend Snippet: Cytotoxicity of LNP-COOH analyzed using ( A ) cell impedance and ( B ) MTT assays in Calu-3 cells. Cytotoxicity MTT assays of LNP-PEP ( C ), LNP-rhACE2 ( D ), LNP-mAb ( E ), and LNP-si ACE2 ( F ) in Calu-3 cells. Values are expressed as the mean ± standard error of the mean (n = 3).

    Techniques Used:

    Quantitative determination of cellular accumulation of ( A ) LNP-PEP and ( B ) LNP-rhACE2 in Calu-3 cells over time. Representative confocal microscopy images from Calu-3 cells treated (3 hours) with cell-impermeable LNP-PEP ( C ‒ F ) and cell-permeable LNP- si ACE2 ( G ‒ J ). Plasma membrane from Calu-3 cells was stained with WGA Alexa Fluor TM 594 ( C and G ); LNP localization in Calu-3 cells was visualized with green fluorescent DiO dye ( D and H ); and cell nuclei were stained using Hoestch ( E and I ). Composite overlay of all three images showing cell membrane, nuclei, and LNPs ( F and J ). Orthogonal projections of Calu-3 cells treated with LNP-si ACE2 ( K ) for 3 hours. Two-dimensional images in z-position where red lines indicate cross-section through y-position and green lines indicate cross-section through x-position. The white dashed box in image K is enlarged. Values are expressed as the mean ± standard error of the mean (n = 3).
    Figure Legend Snippet: Quantitative determination of cellular accumulation of ( A ) LNP-PEP and ( B ) LNP-rhACE2 in Calu-3 cells over time. Representative confocal microscopy images from Calu-3 cells treated (3 hours) with cell-impermeable LNP-PEP ( C ‒ F ) and cell-permeable LNP- si ACE2 ( G ‒ J ). Plasma membrane from Calu-3 cells was stained with WGA Alexa Fluor TM 594 ( C and G ); LNP localization in Calu-3 cells was visualized with green fluorescent DiO dye ( D and H ); and cell nuclei were stained using Hoestch ( E and I ). Composite overlay of all three images showing cell membrane, nuclei, and LNPs ( F and J ). Orthogonal projections of Calu-3 cells treated with LNP-si ACE2 ( K ) for 3 hours. Two-dimensional images in z-position where red lines indicate cross-section through y-position and green lines indicate cross-section through x-position. The white dashed box in image K is enlarged. Values are expressed as the mean ± standard error of the mean (n = 3).

    Techniques Used: Confocal Microscopy, Membrane, Staining

    Relative ACE2 ( A ) and TMPRSS2( B ) mRNA levels in HEK-293-hACE2 cells at different time points after transfection with LNP-si ACE2 (40 nM si ACE2 ) and LNP-si TMPRSS2 (40 nM si TMPRSS2 ), respectively. The control cells in ( A ) and ( B ) received LNP-siSCR. Relative ACE2 mRNA level in Calu-3 cells 72 hours after receiving LNP-si ACE2 (40 nM si ACE2 ) against control cells that received LNP-siSCR ( C ). Relative ACE2 protein level in Calu-3 cells 72 hours after receiving LNP-si ACE2 (40 nM si ACE2 ) against control cells that received LNP-siSCR ( D ). The uncropped blots are shown in . Values are expressed as the mean ± standard error of the mean (n = 3). Statistical significance between the control and treatment groups were analyzed using t -test (**p < 0.01, ***p<0.001, ****p<0.0001; p < 0.05 was considered statistically significant).
    Figure Legend Snippet: Relative ACE2 ( A ) and TMPRSS2( B ) mRNA levels in HEK-293-hACE2 cells at different time points after transfection with LNP-si ACE2 (40 nM si ACE2 ) and LNP-si TMPRSS2 (40 nM si TMPRSS2 ), respectively. The control cells in ( A ) and ( B ) received LNP-siSCR. Relative ACE2 mRNA level in Calu-3 cells 72 hours after receiving LNP-si ACE2 (40 nM si ACE2 ) against control cells that received LNP-siSCR ( C ). Relative ACE2 protein level in Calu-3 cells 72 hours after receiving LNP-si ACE2 (40 nM si ACE2 ) against control cells that received LNP-siSCR ( D ). The uncropped blots are shown in . Values are expressed as the mean ± standard error of the mean (n = 3). Statistical significance between the control and treatment groups were analyzed using t -test (**p < 0.01, ***p<0.001, ****p<0.0001; p < 0.05 was considered statistically significant).

    Techniques Used: Transfection

    Transduction efficiency (luciferase expression) of SARS-CoV-2 S pseudotyped luciferase lentivirus at the given MOIs in HEK-293 and HEK-293-hACE2 cell lines ( A ). Percentage inhibition of infection in ACE2 KD and TMPRSS2 KD HEK-293-hACE2 cells ( B ). Percentage inhibition of infection in ACE2 KD ( C ) and TMPRSS2 KD ( D ) in airway epithelial Calu-3 cell line at different siRNA concentrations (LNP-siRNA: 1, 2, 10, 20 and 40 nM; presented in the graph as log 10 values). The nonlinear regression curve fit analysis of siRNA concentration vs inhibition is presented in the graphs. Values are expressed as the mean ± standard error of the mean (n =3). Statistical analysis was performed using one-way ANOVA, followed by Tukey’s test (*p < 0.05, **p<0.01; p < 0.05 was considered statistically significant).
    Figure Legend Snippet: Transduction efficiency (luciferase expression) of SARS-CoV-2 S pseudotyped luciferase lentivirus at the given MOIs in HEK-293 and HEK-293-hACE2 cell lines ( A ). Percentage inhibition of infection in ACE2 KD and TMPRSS2 KD HEK-293-hACE2 cells ( B ). Percentage inhibition of infection in ACE2 KD ( C ) and TMPRSS2 KD ( D ) in airway epithelial Calu-3 cell line at different siRNA concentrations (LNP-siRNA: 1, 2, 10, 20 and 40 nM; presented in the graph as log 10 values). The nonlinear regression curve fit analysis of siRNA concentration vs inhibition is presented in the graphs. Values are expressed as the mean ± standard error of the mean (n =3). Statistical analysis was performed using one-way ANOVA, followed by Tukey’s test (*p < 0.05, **p<0.01; p < 0.05 was considered statistically significant).

    Techniques Used: Transduction, Luciferase, Expressing, Inhibition, Infection, Concentration Assay

    Percentage inhibition of SARS-CoV-2 S pseudotyped luciferase lentivirus (MOI = 2) infection in Calu-3 cells in the presence of different concentrations (Log ( C ) of ACE2 peptide and LNP-PEP ( A ), rhACE2 and LNP-rhACE2 ( B ), mAb and LNP-mAb ( C ). The nonlinear regression curve fit analysis of concentration vs inhibition is presented in the graphs. Values are expressed as the mean ± standard error of the mean (n = 3).
    Figure Legend Snippet: Percentage inhibition of SARS-CoV-2 S pseudotyped luciferase lentivirus (MOI = 2) infection in Calu-3 cells in the presence of different concentrations (Log ( C ) of ACE2 peptide and LNP-PEP ( A ), rhACE2 and LNP-rhACE2 ( B ), mAb and LNP-mAb ( C ). The nonlinear regression curve fit analysis of concentration vs inhibition is presented in the graphs. Values are expressed as the mean ± standard error of the mean (n = 3).

    Techniques Used: Inhibition, Luciferase, Infection, Concentration Assay

    Percentage inhibition of SARS-Cov-2 S pseudotyped luciferase lentivirus infection (MOI = 2) in airway epithelial Calu-3 cell line following a combination of conditions including LNP-PEP treatment and ACE2 and TMPRSS2 knockdown. The extent of infection was measured following a luciferase assay. Values are expressed as the mean ± standard error of the mean (n = 3). Statistical analysis was performed using one-way ANOVA, followed by Tukey’s test (*p < 0.05, **p < 0.01, ***p<0.001, ****p<0.0001; p < 0.05 was considered statistically significant).
    Figure Legend Snippet: Percentage inhibition of SARS-Cov-2 S pseudotyped luciferase lentivirus infection (MOI = 2) in airway epithelial Calu-3 cell line following a combination of conditions including LNP-PEP treatment and ACE2 and TMPRSS2 knockdown. The extent of infection was measured following a luciferase assay. Values are expressed as the mean ± standard error of the mean (n = 3). Statistical analysis was performed using one-way ANOVA, followed by Tukey’s test (*p < 0.05, **p < 0.01, ***p<0.001, ****p<0.0001; p < 0.05 was considered statistically significant).

    Techniques Used: Inhibition, Luciferase, Infection

    Percentage inhibition of SARS-Cov-2 S pseudotyped luciferase LV infection (MOI = 2) in airway epithelial Calu-3 cell line following treatment with different concentrations of lactoferrin ( A ), camostat mesylate ( B ), and carrageenan ( C ). Percentage inhibition of infection in Calu-3 cells with various combinatory treatments of LNP-PEP (0.1 µg/mL), lactoferrin (10 µM), camostat mesylate (1 µM), and carrageenan (1 µM) ( D ). The extent of infection was measured following a luciferase assay. Values are expressed as the mean ± standard error of the mean (n = 3). Statistical analysis was performed using one-way ANOVA, followed by Tukey’s test (*p < 0.05, ****p<0.0001; p < 0.05 was considered statistically significant).
    Figure Legend Snippet: Percentage inhibition of SARS-Cov-2 S pseudotyped luciferase LV infection (MOI = 2) in airway epithelial Calu-3 cell line following treatment with different concentrations of lactoferrin ( A ), camostat mesylate ( B ), and carrageenan ( C ). Percentage inhibition of infection in Calu-3 cells with various combinatory treatments of LNP-PEP (0.1 µg/mL), lactoferrin (10 µM), camostat mesylate (1 µM), and carrageenan (1 µM) ( D ). The extent of infection was measured following a luciferase assay. Values are expressed as the mean ± standard error of the mean (n = 3). Statistical analysis was performed using one-way ANOVA, followed by Tukey’s test (*p < 0.05, ****p<0.0001; p < 0.05 was considered statistically significant).

    Techniques Used: Inhibition, Luciferase, Infection

    Distribution of LNP-PEP ( A ) and LNP-si ACE2 ( B ) at different time points up to 24 hours after intranasal administration in nude mice. The LNPs were tagged with IR dye (DiR) to aid visualization. The NIR fluorescence images (Ex/Em 745/800) were acquired and analyzed using IVIS imager. The quantitative analysis of fluorescent LNP-PEP in the head ( C ) and body ( D ) regions at different time points. The quantitative analysis of fluorescent LNP-si ACE2 in the head ( E ) and body ( F ) regions at different time points. Values are expressed as the mean ± standard error of the mean (n = 4).
    Figure Legend Snippet: Distribution of LNP-PEP ( A ) and LNP-si ACE2 ( B ) at different time points up to 24 hours after intranasal administration in nude mice. The LNPs were tagged with IR dye (DiR) to aid visualization. The NIR fluorescence images (Ex/Em 745/800) were acquired and analyzed using IVIS imager. The quantitative analysis of fluorescent LNP-PEP in the head ( C ) and body ( D ) regions at different time points. The quantitative analysis of fluorescent LNP-si ACE2 in the head ( E ) and body ( F ) regions at different time points. Values are expressed as the mean ± standard error of the mean (n = 4).

    Techniques Used: Fluorescence

    sars cov 2 spike rbd ace2 blocking antibody detection elisa kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc sars cov 2 spike rbd ace2 blocking antibody detection elisa kit
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    sars cov 2 rbd e7b3e rabbit monoclonal antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc sars cov 2 rbd e7b3e rabbit monoclonal antibody
    <t>SARS-CoV-2</t> receptor-binding domain (RBD) protein expression by Group#1 overexpression vectors.
    Sars Cov 2 Rbd E7b3e Rabbit Monoclonal Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sars cov 2 rbd e7b3e rabbit monoclonal antibody/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sars cov 2 rbd e7b3e rabbit monoclonal antibody - by Bioz Stars, 2024-07
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    1) Product Images from "Novel Enhanced Mammalian Cell Transient Expression Vector via Promoter Combination"

    Article Title: Novel Enhanced Mammalian Cell Transient Expression Vector via Promoter Combination

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms25042330

    SARS-CoV-2 receptor-binding domain (RBD) protein expression by Group#1 overexpression vectors.
    Figure Legend Snippet: SARS-CoV-2 receptor-binding domain (RBD) protein expression by Group#1 overexpression vectors.

    Techniques Used: Binding Assay, Expressing, Over Expression

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    SARS-CoV-2 receptor-binding domain (RBD) protein expression by Group#1 overexpression vectors.

    Journal: International Journal of Molecular Sciences

    Article Title: Novel Enhanced Mammalian Cell Transient Expression Vector via Promoter Combination

    doi: 10.3390/ijms25042330

    Figure Lengend Snippet: SARS-CoV-2 receptor-binding domain (RBD) protein expression by Group#1 overexpression vectors.

    Article Snippet: The membrane was blocked using EveryBlot blocking buffer (12010020, Bio-Rad, Hercules, CA, USA) and probed with a SARS-CoV-2 RBD (E7B3E) rabbit monoclonal antibody (63847S, Cell Signaling Technology, Danvers, MA, USA).

    Techniques: Binding Assay, Expressing, Over Expression