ã â 1 4 rb (Worthington Biochemical)


Name:
Trypsin
Description:
Supplied as dialyzed and lyophilized powder
Catalog Number:
ls003702
Price:
23
Size:
100 mg
Source:
Bovine Pancreas
Cas Number:
9002.07.7
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Structured Review

Supplied as dialyzed and lyophilized powder
https://www.bioz.com/result/ã â 1 4 rb/product/Worthington Biochemical
Average 90 stars, based on 170 article reviews
Price from $9.99 to $1999.99
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1) Product Images from "Gelatin-Based Microribbon Hydrogels Accelerate Cartilage Formation by Mesenchymal Stem Cells in Three Dimensions"
Article Title: Gelatin-Based Microribbon Hydrogels Accelerate Cartilage Formation by Mesenchymal Stem Cells in Three Dimensions
Journal: Tissue Engineering. Part A
doi: 10.1089/ten.tea.2018.0011

Figure Legend Snippet: üRB scaffolds, but not HGs, led to accelerated increases in compressive moduli of MSC-based neocartilage formation after 21 days of culture in chondrogenic medium in vitro . (A) MSC-seeded üRBs or HG samples showed increased opacity over time, suggesting matrix deposition by MSCs in 3D. Acellular üRB scaffolds degraded and lost integrity over time while no significant change was observed in acellular HG controls. (B) Unconfined compressive testing revealed a 20-fold increase in compressive modulus in MSC-seeded üRBs, while only a three-fold increase was observed in MSC-seeded HGs. The moduli of acellular üRB scaffolds decreased significantly while the compressive modulus of acellular HGs remained the same. Scale bar: 2âÂÂmm. ns, nonsignificant ( p â > âÂÂ0.05), ** p âÂÂ
Techniques Used: In Vitro

Figure Legend Snippet: The üRB scaffolds led to significantly enhanced MSC proliferation and cartilage matrix production compared with HGs in 3D. (A) DNA content per wet weight increased â¼3-fold in üRB scaffolds, and no significant change in DNA was observed in HGs. (B) Quantitative GAG assay showed both üRB and HG scaffolds led to significantly enhanced GAG deposition by MSCs over 21 days, with üRB scaffolds leading to â¼50% more total sGAG deposition than HG scaffolds. nsâÂÂ=âÂÂnonsignificant ( p â > âÂÂ0.05), ** p âÂÂ
Techniques Used:
Related Articles
Incubation:Article Title: Conformational Changes in the Spike Glycoprotein of Murine Coronavirus Are Induced at 37?C either by Soluble Murine CEACAM1 Receptors or by pH 8 Article Snippet: .. Incubation of MHV-A59 virions with smCEACAM1a[1-4] receptor glycoprotein at 4°C did not make the S2 protein susceptible to degradation by other:Article Title: Conformational Changes in the Spike Glycoprotein of Murine Coronavirus Are Induced at 37?C either by Soluble Murine CEACAM1 Receptors or by pH 8 Article Snippet: The conformational changes in S induced at 37°C by soluble receptor or pH 8.0 were apparently irreversible, since cooling the treated virions to 4°C and incubating for a prolonged time did not prevent subsequent degradation of S2 by trypsin-TPCK at 4°C. Article Title: Unique Type I Interferon Responses Determine the Functional Fate of Migratory Lung Dendritic Cells during Influenza Virus Infection Article Snippet: Supporting Information Migratory DCs can transfer infectious virus to uninfected cells in the absence of TPCK-trypsin. Article Title: Conformational Changes in the Spike Glycoprotein of Murine Coronavirus Are Induced at 37?C either by Soluble Murine CEACAM1 Receptors or by pH 8 Article Snippet: As anticipated, at 37°C and pH 6.5, smCEACAM1a[1-4] induced a conformational change in the MHV-A59 spike in the SA59 R virus that made the S2 protein susceptible to degradation by trypsin-TPCK (Fig. ). smCEACAM1b[1-4] also triggered the conformational change in S2 at 37°C but did so less efficiently than did smCEACAM1a[1-4] (Fig. ). Article Title: Exploring protein interfaces with a general photochemical reagent Article Snippet: Complete enzymatic digestion of these carbamidomethylated samples with TPCK trypsin was achieved in NH4 CO3 H (0.1 M at pH 8.0), after 12–24 h at 37°C using a 2% (w/w) enzyme:substrate ratio. Mouse Assay:Article Title: Unique Type I Interferon Responses Determine the Functional Fate of Migratory Lung Dendritic Cells during Influenza Virus Infection Article Snippet: .. A. MLN-DCs (gate V, ) from PR8 or WSN infected mice (day 3) were co-cultured with MDCK cells in the presence or absence of Article Title: Unique Type I Interferon Responses Determine the Functional Fate of Migratory Lung Dendritic Cells during Influenza Virus Infection Article Snippet: .. B. Sorted CD103+ DCs and CD11bhigh DCs from PR8 or WSN-infected mice were co-cultured with MDCK cells in the presence or absence of Infection:Article Title: Unique Type I Interferon Responses Determine the Functional Fate of Migratory Lung Dendritic Cells during Influenza Virus Infection Article Snippet: .. A. MLN-DCs (gate V, ) from PR8 or WSN infected mice (day 3) were co-cultured with MDCK cells in the presence or absence of Purification:Article Title: The analysis of macromolecular interactions by sedimentation equilibrium Article Snippet: .. Purified α-chymotrypsin (Cat. No. 1475) and |