㠎 â 1 4 rb  (Worthington Biochemical)


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  • 84
    Name:
    Trypsin
    Description:
    Supplied as dialyzed and lyophilized powder
    Catalog Number:
    ls003702
    Price:
    23
    Size:
    100 mg
    Source:
    Bovine Pancreas
    Cas Number:
    9002.07.7
    Buy from Supplier


    Structured Review

    Worthington Biochemical 㠎 â 1 4 rb
    <t>μRB</t> scaffolds, but not HGs, led to accelerated increases in compressive moduli of MSC-based neocartilage formation after 21 days of culture in chondrogenic medium in vitro . (A) MSC-seeded μRBs or HG samples showed increased opacity over time, suggesting matrix deposition by MSCs in 3D. Acellular μRB scaffolds degraded and lost integrity over time while no significant change was observed in acellular HG controls. (B) Unconfined compressive testing revealed a 20-fold increase in compressive modulus in MSC-seeded μRBs, while only a three-fold increase was observed in MSC-seeded HGs. The moduli of acellular μRB scaffolds decreased significantly while the compressive modulus of acellular HGs remained the same. Scale bar: 2 mm. ns, nonsignificant ( p <t> </t> >  0.05), ** p  
    Supplied as dialyzed and lyophilized powder
    https://www.bioz.com/result/㠎 â 1 4 rb/product/Worthington Biochemical
    Average 84 stars, based on 170 article reviews
    Price from $9.99 to $1999.99
    㠎 â 1 4 rb - by Bioz Stars, 2020-08
    84/100 stars

    Images

    1) Product Images from "Gelatin-Based Microribbon Hydrogels Accelerate Cartilage Formation by Mesenchymal Stem Cells in Three Dimensions"

    Article Title: Gelatin-Based Microribbon Hydrogels Accelerate Cartilage Formation by Mesenchymal Stem Cells in Three Dimensions

    Journal: Tissue Engineering. Part A

    doi: 10.1089/ten.tea.2018.0011

    μRB scaffolds, but not HGs, led to accelerated increases in compressive moduli of MSC-based neocartilage formation after 21 days of culture in chondrogenic medium in vitro . (A) MSC-seeded μRBs or HG samples showed increased opacity over time, suggesting matrix deposition by MSCs in 3D. Acellular μRB scaffolds degraded and lost integrity over time while no significant change was observed in acellular HG controls. (B) Unconfined compressive testing revealed a 20-fold increase in compressive modulus in MSC-seeded μRBs, while only a three-fold increase was observed in MSC-seeded HGs. The moduli of acellular μRB scaffolds decreased significantly while the compressive modulus of acellular HGs remained the same. Scale bar: 2 mm. ns, nonsignificant ( p   >  0.05), ** p  
    Figure Legend Snippet: μRB scaffolds, but not HGs, led to accelerated increases in compressive moduli of MSC-based neocartilage formation after 21 days of culture in chondrogenic medium in vitro . (A) MSC-seeded μRBs or HG samples showed increased opacity over time, suggesting matrix deposition by MSCs in 3D. Acellular μRB scaffolds degraded and lost integrity over time while no significant change was observed in acellular HG controls. (B) Unconfined compressive testing revealed a 20-fold increase in compressive modulus in MSC-seeded μRBs, while only a three-fold increase was observed in MSC-seeded HGs. The moduli of acellular μRB scaffolds decreased significantly while the compressive modulus of acellular HGs remained the same. Scale bar: 2 mm. ns, nonsignificant ( p   >  0.05), ** p  

    Techniques Used: In Vitro

    The μRB scaffolds led to significantly enhanced MSC proliferation and cartilage matrix production compared with HGs in 3D. (A) DNA content per wet weight increased ∼3-fold in μRB scaffolds, and no significant change in DNA was observed in HGs. (B) Quantitative GAG assay showed both μRB and HG scaffolds led to significantly enhanced GAG deposition by MSCs over 21 days, with μRB scaffolds leading to ∼50% more total sGAG deposition than HG scaffolds. ns = nonsignificant ( p   >  0.05), ** p  
    Figure Legend Snippet: The μRB scaffolds led to significantly enhanced MSC proliferation and cartilage matrix production compared with HGs in 3D. (A) DNA content per wet weight increased ∼3-fold in μRB scaffolds, and no significant change in DNA was observed in HGs. (B) Quantitative GAG assay showed both μRB and HG scaffolds led to significantly enhanced GAG deposition by MSCs over 21 days, with μRB scaffolds leading to ∼50% more total sGAG deposition than HG scaffolds. ns = nonsignificant ( p   >  0.05), ** p  

    Techniques Used:

    Related Articles

    Incubation:

    Article Title: Conformational Changes in the Spike Glycoprotein of Murine Coronavirus Are Induced at 37?C either by Soluble Murine CEACAM1 Receptors or by pH 8
    Article Snippet: .. Incubation of MHV-A59 virions with smCEACAM1a[1-4] receptor glycoprotein at 4°C did not make the S2 protein susceptible to degradation by trypsin-TPCK at 4°C (Fig. ). ..

    other:

    Article Title: Conformational Changes in the Spike Glycoprotein of Murine Coronavirus Are Induced at 37?C either by Soluble Murine CEACAM1 Receptors or by pH 8
    Article Snippet: The conformational changes in S induced at 37°C by soluble receptor or pH 8.0 were apparently irreversible, since cooling the treated virions to 4°C and incubating for a prolonged time did not prevent subsequent degradation of S2 by trypsin-TPCK at 4°C.

    Article Title: Unique Type I Interferon Responses Determine the Functional Fate of Migratory Lung Dendritic Cells during Influenza Virus Infection
    Article Snippet: Supporting Information Migratory DCs can transfer infectious virus to uninfected cells in the absence of TPCK-trypsin.

    Article Title: Conformational Changes in the Spike Glycoprotein of Murine Coronavirus Are Induced at 37?C either by Soluble Murine CEACAM1 Receptors or by pH 8
    Article Snippet: As anticipated, at 37°C and pH 6.5, smCEACAM1a[1-4] induced a conformational change in the MHV-A59 spike in the SA59 R virus that made the S2 protein susceptible to degradation by trypsin-TPCK (Fig. ). smCEACAM1b[1-4] also triggered the conformational change in S2 at 37°C but did so less efficiently than did smCEACAM1a[1-4] (Fig. ).

    Article Title: Exploring protein interfaces with a general photochemical reagent
    Article Snippet: Complete enzymatic digestion of these carbamidomethylated samples with TPCK trypsin was achieved in NH4 CO3 H (0.1 M at pH 8.0), after 12–24 h at 37°C using a 2% (w/w) enzyme:substrate ratio.

    Mouse Assay:

    Article Title: Unique Type I Interferon Responses Determine the Functional Fate of Migratory Lung Dendritic Cells during Influenza Virus Infection
    Article Snippet: .. A. MLN-DCs (gate V, ) from PR8 or WSN infected mice (day 3) were co-cultured with MDCK cells in the presence or absence of TPCK-trypsin for 2 days. .. Virus replication was assessed by HRP-based immunostaining of MDCK cells with a chicken polyclonal antibody to influenza virus.

    Article Title: Unique Type I Interferon Responses Determine the Functional Fate of Migratory Lung Dendritic Cells during Influenza Virus Infection
    Article Snippet: .. B. Sorted CD103+ DCs and CD11bhigh DCs from PR8 or WSN-infected mice were co-cultured with MDCK cells in the presence or absence of TPCK-trypsin and the culture supernatants were assayed for infectious virus particles at day 2 by hemmaglutination of RBCs. (PDF) Click here for additional data file. .. Sorting strategy to isolate LN-resident CD8α+ DCs during influenza virus infection.

    Infection:

    Article Title: Unique Type I Interferon Responses Determine the Functional Fate of Migratory Lung Dendritic Cells during Influenza Virus Infection
    Article Snippet: .. A. MLN-DCs (gate V, ) from PR8 or WSN infected mice (day 3) were co-cultured with MDCK cells in the presence or absence of TPCK-trypsin for 2 days. .. Virus replication was assessed by HRP-based immunostaining of MDCK cells with a chicken polyclonal antibody to influenza virus.

    Purification:

    Article Title: The analysis of macromolecular interactions by sedimentation equilibrium
    Article Snippet: .. Purified α-chymotrypsin (Cat. No. 1475) and soybean trypsin inhibitor (STI) (Cat. No. 3570) were purchased from Worthington Biochemical Corporation. .. Approximately 21 mg of each were dissolved in 0.2M sodium chloride and 0.05M sodium phosphate (pH = 6.7) and further purified by size exclusion chromatography on a HiPrep 16/60 Sephacryl S-200 HR column (Cat. No. 17-1166-01, GE Healthcare) at 0.5 mL/min in the same buffer.

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  • 84
    Worthington Biochemical 㠎 â 1 4 rb
    <t>μRB</t> scaffolds, but not HGs, led to accelerated increases in compressive moduli of MSC-based neocartilage formation after 21 days of culture in chondrogenic medium in vitro . (A) MSC-seeded μRBs or HG samples showed increased opacity over time, suggesting matrix deposition by MSCs in 3D. Acellular μRB scaffolds degraded and lost integrity over time while no significant change was observed in acellular HG controls. (B) Unconfined compressive testing revealed a 20-fold increase in compressive modulus in MSC-seeded μRBs, while only a three-fold increase was observed in MSC-seeded HGs. The moduli of acellular μRB scaffolds decreased significantly while the compressive modulus of acellular HGs remained the same. Scale bar: 2 mm. ns, nonsignificant ( p <t> </t> >  0.05), ** p  
    㠎 â 1 4 Rb, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/㠎 â 1 4 rb/product/Worthington Biochemical
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    㠎 â 1 4 rb - by Bioz Stars, 2020-08
    84/100 stars
      Buy from Supplier

    91
    Worthington Biochemical warren rb
    <t>μRB</t> scaffolds, but not HGs, led to accelerated increases in compressive moduli of MSC-based neocartilage formation after 21 days of culture in chondrogenic medium in vitro . (A) MSC-seeded μRBs or HG samples showed increased opacity over time, suggesting matrix deposition by MSCs in 3D. Acellular μRB scaffolds degraded and lost integrity over time while no significant change was observed in acellular HG controls. (B) Unconfined compressive testing revealed a 20-fold increase in compressive modulus in MSC-seeded μRBs, while only a three-fold increase was observed in MSC-seeded HGs. The moduli of acellular μRB scaffolds decreased significantly while the compressive modulus of acellular HGs remained the same. Scale bar: 2 mm. ns, nonsignificant ( p <t> </t> >  0.05), ** p  
    Warren Rb, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/warren rb/product/Worthington Biochemical
    Average 91 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    warren rb - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    85
    Worthington Biochemical prosek rb
    <t>μRB</t> scaffolds, but not HGs, led to accelerated increases in compressive moduli of MSC-based neocartilage formation after 21 days of culture in chondrogenic medium in vitro . (A) MSC-seeded μRBs or HG samples showed increased opacity over time, suggesting matrix deposition by MSCs in 3D. Acellular μRB scaffolds degraded and lost integrity over time while no significant change was observed in acellular HG controls. (B) Unconfined compressive testing revealed a 20-fold increase in compressive modulus in MSC-seeded μRBs, while only a three-fold increase was observed in MSC-seeded HGs. The moduli of acellular μRB scaffolds decreased significantly while the compressive modulus of acellular HGs remained the same. Scale bar: 2 mm. ns, nonsignificant ( p <t> </t> >  0.05), ** p  
    Prosek Rb, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/prosek rb/product/Worthington Biochemical
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    prosek rb - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    Image Search Results


    μRB scaffolds, but not HGs, led to accelerated increases in compressive moduli of MSC-based neocartilage formation after 21 days of culture in chondrogenic medium in vitro . (A) MSC-seeded μRBs or HG samples showed increased opacity over time, suggesting matrix deposition by MSCs in 3D. Acellular μRB scaffolds degraded and lost integrity over time while no significant change was observed in acellular HG controls. (B) Unconfined compressive testing revealed a 20-fold increase in compressive modulus in MSC-seeded μRBs, while only a three-fold increase was observed in MSC-seeded HGs. The moduli of acellular μRB scaffolds decreased significantly while the compressive modulus of acellular HGs remained the same. Scale bar: 2 mm. ns, nonsignificant ( p   >  0.05), ** p  

    Journal: Tissue Engineering. Part A

    Article Title: Gelatin-Based Microribbon Hydrogels Accelerate Cartilage Formation by Mesenchymal Stem Cells in Three Dimensions

    doi: 10.1089/ten.tea.2018.0011

    Figure Lengend Snippet: μRB scaffolds, but not HGs, led to accelerated increases in compressive moduli of MSC-based neocartilage formation after 21 days of culture in chondrogenic medium in vitro . (A) MSC-seeded μRBs or HG samples showed increased opacity over time, suggesting matrix deposition by MSCs in 3D. Acellular μRB scaffolds degraded and lost integrity over time while no significant change was observed in acellular HG controls. (B) Unconfined compressive testing revealed a 20-fold increase in compressive modulus in MSC-seeded μRBs, while only a three-fold increase was observed in MSC-seeded HGs. The moduli of acellular μRB scaffolds decreased significantly while the compressive modulus of acellular HGs remained the same. Scale bar: 2 mm. ns, nonsignificant ( p   >  0.05), ** p  

    Article Snippet: The masses of the μRB and HG scaffolds ( n  = 5) were measured wet (prelyophilized) and dry (postlyophilized) before samples were digested in papainase solution (Worthington Biochemical, Lakewood, NJ) at 60°C for 16 h.

    Techniques: In Vitro

    The μRB scaffolds led to significantly enhanced MSC proliferation and cartilage matrix production compared with HGs in 3D. (A) DNA content per wet weight increased ∼3-fold in μRB scaffolds, and no significant change in DNA was observed in HGs. (B) Quantitative GAG assay showed both μRB and HG scaffolds led to significantly enhanced GAG deposition by MSCs over 21 days, with μRB scaffolds leading to ∼50% more total sGAG deposition than HG scaffolds. ns = nonsignificant ( p   >  0.05), ** p  

    Journal: Tissue Engineering. Part A

    Article Title: Gelatin-Based Microribbon Hydrogels Accelerate Cartilage Formation by Mesenchymal Stem Cells in Three Dimensions

    doi: 10.1089/ten.tea.2018.0011

    Figure Lengend Snippet: The μRB scaffolds led to significantly enhanced MSC proliferation and cartilage matrix production compared with HGs in 3D. (A) DNA content per wet weight increased ∼3-fold in μRB scaffolds, and no significant change in DNA was observed in HGs. (B) Quantitative GAG assay showed both μRB and HG scaffolds led to significantly enhanced GAG deposition by MSCs over 21 days, with μRB scaffolds leading to ∼50% more total sGAG deposition than HG scaffolds. ns = nonsignificant ( p   >  0.05), ** p  

    Article Snippet: The masses of the μRB and HG scaffolds ( n  = 5) were measured wet (prelyophilized) and dry (postlyophilized) before samples were digested in papainase solution (Worthington Biochemical, Lakewood, NJ) at 60°C for 16 h.

    Techniques: