sirna transfection raw264 7 macrophages  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH sirna transfection raw264 7 macrophages
    Sirna Transfection Raw264 7 Macrophages, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    raw264 7 cells  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH raw264 7 cells
    Intracellular reactive oxygen species (ROS)-scavenging effect of G-TSrP and release profile of TA and Sr 2+ . 2′,7′-dichlorofluorescein diacetate (DCFDA) assay images and their quantification using (a) HDFBs (scale bar: 100 μm) and (b) <t>RAW264.7</t> cells (scale bar: 50 μm) treated with different hydrogel extracts and H 2 O 2 . (c) DCFDA assay images and their quantification using RAW264.7 cells after treatment of hydrogel extracts and lipopolysaccharide (LPS). Scale bar: 50 μm. (d) Release profile of TA and Sr 2+ from G-TSrP for 14 days.
    Raw264 7 Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Cell-homing and immunomodulatory composite hydrogels for effective wound healing with neovascularization"

    Article Title: Cell-homing and immunomodulatory composite hydrogels for effective wound healing with neovascularization

    Journal: Bioactive Materials

    doi: 10.1016/j.bioactmat.2024.02.029

    Intracellular reactive oxygen species (ROS)-scavenging effect of G-TSrP and release profile of TA and Sr 2+ . 2′,7′-dichlorofluorescein diacetate (DCFDA) assay images and their quantification using (a) HDFBs (scale bar: 100 μm) and (b) RAW264.7 cells (scale bar: 50 μm) treated with different hydrogel extracts and H 2 O 2 . (c) DCFDA assay images and their quantification using RAW264.7 cells after treatment of hydrogel extracts and lipopolysaccharide (LPS). Scale bar: 50 μm. (d) Release profile of TA and Sr 2+ from G-TSrP for 14 days.
    Figure Legend Snippet: Intracellular reactive oxygen species (ROS)-scavenging effect of G-TSrP and release profile of TA and Sr 2+ . 2′,7′-dichlorofluorescein diacetate (DCFDA) assay images and their quantification using (a) HDFBs (scale bar: 100 μm) and (b) RAW264.7 cells (scale bar: 50 μm) treated with different hydrogel extracts and H 2 O 2 . (c) DCFDA assay images and their quantification using RAW264.7 cells after treatment of hydrogel extracts and lipopolysaccharide (LPS). Scale bar: 50 μm. (d) Release profile of TA and Sr 2+ from G-TSrP for 14 days.

    Techniques Used:

    Modulation of macrophage polarization by G-TSrP. (a) Percentage of iNOS-positive cells from immunofluorescence (IF) images of RAW264.7 cells after treatment with LPS and hydrogel extracts. (b) F-actin staining images of RAW264.7 cells after treatment with LPS and hydrogel extracts, along with measurement of their cell area. Scale bar: 25 μm. (c) Percentage of CD206-positive cells from IF images of RAW264.7 cells after treatment with interleukin (IL)-4 and hydrogel extracts. (d) F-actin staining images of RAW264.7 cells after treatment with IL-4 and hydrogel extracts, along with measurement of their aspect ratio. Scale bar: 25 μm. Relative gene expression of (e) M1 polarization markers and (f) M2 polarization markers of RAW264.7 cells cultured using hydrogel extracts with their induction factors (M1: LPS, M2: IL-4).
    Figure Legend Snippet: Modulation of macrophage polarization by G-TSrP. (a) Percentage of iNOS-positive cells from immunofluorescence (IF) images of RAW264.7 cells after treatment with LPS and hydrogel extracts. (b) F-actin staining images of RAW264.7 cells after treatment with LPS and hydrogel extracts, along with measurement of their cell area. Scale bar: 25 μm. (c) Percentage of CD206-positive cells from IF images of RAW264.7 cells after treatment with interleukin (IL)-4 and hydrogel extracts. (d) F-actin staining images of RAW264.7 cells after treatment with IL-4 and hydrogel extracts, along with measurement of their aspect ratio. Scale bar: 25 μm. Relative gene expression of (e) M1 polarization markers and (f) M2 polarization markers of RAW264.7 cells cultured using hydrogel extracts with their induction factors (M1: LPS, M2: IL-4).

    Techniques Used: Immunofluorescence, Staining, Expressing, Cell Culture

    In vivo and in vitro cell homing and biodegradation of composite hydrogels. (a) Hematoxylin and eosin (H&E) staining images (scale bar: 250 μm) and their magnified images (scale bar: 50 μm) of mouse subcutaneous tissues 14 days after hydrogel implantation. (b) Representative dual IF staining (F4/80 and iNOS) images (scale bar: 250 μm) and their magnified images (scale bar: 50 μm) of mouse subcutaneous tissues 14 days after implantation, along with their quantification. (c) Representative dual IF staining (F4/80 and CD206) images (scale bar: 250 μm) and their magnified images (scale bar: 50 μm) of mouse subcutaneous tissues 14 days after implantation, along with their quantification. (d) Images of migrated RAW264.7 cells cultured with GelMA and G-TSrP hydrogel for 24 h and their quantification. Scale bar: 250 μm. (e) Relative gene expression of MMP9 and MMP13 of RAW264.7 cells cultured with IL-4 and each hydrogel extract.
    Figure Legend Snippet: In vivo and in vitro cell homing and biodegradation of composite hydrogels. (a) Hematoxylin and eosin (H&E) staining images (scale bar: 250 μm) and their magnified images (scale bar: 50 μm) of mouse subcutaneous tissues 14 days after hydrogel implantation. (b) Representative dual IF staining (F4/80 and iNOS) images (scale bar: 250 μm) and their magnified images (scale bar: 50 μm) of mouse subcutaneous tissues 14 days after implantation, along with their quantification. (c) Representative dual IF staining (F4/80 and CD206) images (scale bar: 250 μm) and their magnified images (scale bar: 50 μm) of mouse subcutaneous tissues 14 days after implantation, along with their quantification. (d) Images of migrated RAW264.7 cells cultured with GelMA and G-TSrP hydrogel for 24 h and their quantification. Scale bar: 250 μm. (e) Relative gene expression of MMP9 and MMP13 of RAW264.7 cells cultured with IL-4 and each hydrogel extract.

    Techniques Used: In Vivo, In Vitro, Staining, Cell Culture, Expressing

    Genetic analysis of macrophage-mediated inflammatory wound healing. (a) Total heatmap data of RAW264.7 cells after treatment of different hydrogel extracts in the presence of LPS. (b) Volcano plots representing gene expression in the G-TSP group compared with the GelMA group. (c) Principal component analysis (PCA) plot displaying the variances of the genes in macrophages of GelMA, TA and G-TSrP group. (d) Up-regulated and down-regulated gene ontology (GO) analysis. (e) Schematic illustration of the mechanism of macrophage-mediated wound healing process promoted by G-TSrP. GO enrichment analysis of (f) positive regulation of immune response, (g) cell migration, and (h) angiogenesis.
    Figure Legend Snippet: Genetic analysis of macrophage-mediated inflammatory wound healing. (a) Total heatmap data of RAW264.7 cells after treatment of different hydrogel extracts in the presence of LPS. (b) Volcano plots representing gene expression in the G-TSP group compared with the GelMA group. (c) Principal component analysis (PCA) plot displaying the variances of the genes in macrophages of GelMA, TA and G-TSrP group. (d) Up-regulated and down-regulated gene ontology (GO) analysis. (e) Schematic illustration of the mechanism of macrophage-mediated wound healing process promoted by G-TSrP. GO enrichment analysis of (f) positive regulation of immune response, (g) cell migration, and (h) angiogenesis.

    Techniques Used: Expressing, Migration

    raw264 7 murine macrophage cells  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH raw264 7 murine macrophage cells
    Raw264 7 Murine Macrophage Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    sirna transfection raw264 7 macrophages  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH sirna transfection raw264 7 macrophages
    Sirna Transfection Raw264 7 Macrophages, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    raw264 7 cells  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH raw264 7 cells
    Raw264 7 Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    raw264 7 macrophages  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH raw264 7 macrophages
    Gene expression of Acp-5 ( a ), release of TRAP ( b ) and CaP resorption ( c ) with normal salt (NS) and high salt treatment (HS) in murine osteoclasts differentiated from <t>RAW264.7</t> macrophages (n ≥ 6). Differentiation of osteoclasts was induced by treatment with M-CSF (30 ng/mL) and RANKL (50 ng/mL) for five days, followed by addition of 40 mM NaCl to the HS group. Symbols represent single data points, horizontal lines the arithmetic mean and vertical lines the standard error of the mean. AU: arbitrary units. Statistics: Two-tailed unpaired t-test. ** p < 0.01; *** p < 0.001.
    Raw264 7 Macrophages, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Dietary Salt Accelerates Orthodontic Tooth Movement by Increased Osteoclast Activity"

    Article Title: Dietary Salt Accelerates Orthodontic Tooth Movement by Increased Osteoclast Activity

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms22020596

    Gene expression of Acp-5 ( a ), release of TRAP ( b ) and CaP resorption ( c ) with normal salt (NS) and high salt treatment (HS) in murine osteoclasts differentiated from RAW264.7 macrophages (n ≥ 6). Differentiation of osteoclasts was induced by treatment with M-CSF (30 ng/mL) and RANKL (50 ng/mL) for five days, followed by addition of 40 mM NaCl to the HS group. Symbols represent single data points, horizontal lines the arithmetic mean and vertical lines the standard error of the mean. AU: arbitrary units. Statistics: Two-tailed unpaired t-test. ** p < 0.01; *** p < 0.001.
    Figure Legend Snippet: Gene expression of Acp-5 ( a ), release of TRAP ( b ) and CaP resorption ( c ) with normal salt (NS) and high salt treatment (HS) in murine osteoclasts differentiated from RAW264.7 macrophages (n ≥ 6). Differentiation of osteoclasts was induced by treatment with M-CSF (30 ng/mL) and RANKL (50 ng/mL) for five days, followed by addition of 40 mM NaCl to the HS group. Symbols represent single data points, horizontal lines the arithmetic mean and vertical lines the standard error of the mean. AU: arbitrary units. Statistics: Two-tailed unpaired t-test. ** p < 0.01; *** p < 0.001.

    Techniques Used: Expressing, Two Tailed Test

    murine macrophage cell line raw264 7  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH murine macrophage cell line raw264 7
    (a) Cytotoxic effects of GIE on <t>RAW264.7</t> cells. Cells were treated with different concentrations of GIE (100-500 μ g/mL) for 24 h. MTT assay was used to determine cell viability. Values are expressed as a percentage of the control. (b) GIE attenuated the intracellular ROS production in LPS plus IFN- γ -induced RAW264.7 cells. The intracellular ROS levels are expressed as a percentage of the control. (c) The effects of GIE on antioxidant mRNA expression in LPS plus IFN- γ -induced RAW264.7 cells. (d) GIE suppressed NO production in LPS plus IFN- γ -induced RAW264.7 cells. Nitrite concentration was determined from a sodium nitrite standard curve, and the results are expressed as a concentration ( μ M) of nitrite in a culture medium. (e) The effects of GIE on COX-2 and iNOS mRNA expression in LPS plus IFN- γ -induced RAW264.7 cells. The data represent the mean ± S.D. of two independent experiments. Cells were pretreated with GIE, NAC, or DEX for 3 h and then coincubated with LPS plus IFN- γ for 24 h. UN: uninduced cells; IN: untreated LPS plus IFN- γ -induced cells; NAC: cells were pretreated with NAC at 3 mM; DEX: cells were pretreated with DEX at 1 μ M; GIE (50, 100, 200, and 300): cells were pretreated with GIE at concentrations range of 50, 100, 200, and 300 μ g/mL, respectively. One-way ANOVA performed the comparison, and Tukey was used as a post hoc test. The degree of significance was denoted with different letters for the comparison between sample groups. p < 0.05 was considered as statistically significant.
    Murine Macrophage Cell Line Raw264 7, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Gymnema inodorum (Lour.) Decne. Extract Alleviates Oxidative Stress and Inflammatory Mediators Produced by RAW264.7 Macrophages"

    Article Title: Gymnema inodorum (Lour.) Decne. Extract Alleviates Oxidative Stress and Inflammatory Mediators Produced by RAW264.7 Macrophages

    Journal: Oxidative Medicine and Cellular Longevity

    doi: 10.1155/2021/8658314

    (a) Cytotoxic effects of GIE on RAW264.7 cells. Cells were treated with different concentrations of GIE (100-500 μ g/mL) for 24 h. MTT assay was used to determine cell viability. Values are expressed as a percentage of the control. (b) GIE attenuated the intracellular ROS production in LPS plus IFN- γ -induced RAW264.7 cells. The intracellular ROS levels are expressed as a percentage of the control. (c) The effects of GIE on antioxidant mRNA expression in LPS plus IFN- γ -induced RAW264.7 cells. (d) GIE suppressed NO production in LPS plus IFN- γ -induced RAW264.7 cells. Nitrite concentration was determined from a sodium nitrite standard curve, and the results are expressed as a concentration ( μ M) of nitrite in a culture medium. (e) The effects of GIE on COX-2 and iNOS mRNA expression in LPS plus IFN- γ -induced RAW264.7 cells. The data represent the mean ± S.D. of two independent experiments. Cells were pretreated with GIE, NAC, or DEX for 3 h and then coincubated with LPS plus IFN- γ for 24 h. UN: uninduced cells; IN: untreated LPS plus IFN- γ -induced cells; NAC: cells were pretreated with NAC at 3 mM; DEX: cells were pretreated with DEX at 1 μ M; GIE (50, 100, 200, and 300): cells were pretreated with GIE at concentrations range of 50, 100, 200, and 300 μ g/mL, respectively. One-way ANOVA performed the comparison, and Tukey was used as a post hoc test. The degree of significance was denoted with different letters for the comparison between sample groups. p < 0.05 was considered as statistically significant.
    Figure Legend Snippet: (a) Cytotoxic effects of GIE on RAW264.7 cells. Cells were treated with different concentrations of GIE (100-500 μ g/mL) for 24 h. MTT assay was used to determine cell viability. Values are expressed as a percentage of the control. (b) GIE attenuated the intracellular ROS production in LPS plus IFN- γ -induced RAW264.7 cells. The intracellular ROS levels are expressed as a percentage of the control. (c) The effects of GIE on antioxidant mRNA expression in LPS plus IFN- γ -induced RAW264.7 cells. (d) GIE suppressed NO production in LPS plus IFN- γ -induced RAW264.7 cells. Nitrite concentration was determined from a sodium nitrite standard curve, and the results are expressed as a concentration ( μ M) of nitrite in a culture medium. (e) The effects of GIE on COX-2 and iNOS mRNA expression in LPS plus IFN- γ -induced RAW264.7 cells. The data represent the mean ± S.D. of two independent experiments. Cells were pretreated with GIE, NAC, or DEX for 3 h and then coincubated with LPS plus IFN- γ for 24 h. UN: uninduced cells; IN: untreated LPS plus IFN- γ -induced cells; NAC: cells were pretreated with NAC at 3 mM; DEX: cells were pretreated with DEX at 1 μ M; GIE (50, 100, 200, and 300): cells were pretreated with GIE at concentrations range of 50, 100, 200, and 300 μ g/mL, respectively. One-way ANOVA performed the comparison, and Tukey was used as a post hoc test. The degree of significance was denoted with different letters for the comparison between sample groups. p < 0.05 was considered as statistically significant.

    Techniques Used: MTT Assay, Expressing, Concentration Assay

    GIE suppressed the secretion of (a) proinflammatory cytokines IL-6, (b) TNF- α , and slightly increased (c) anti-inflammatory cytokines IL-10 secretion in LPS plus IFN- γ -induced RAW264.7 cells. The effects of GIE on (d) IL-6 and (e) TNF- α mRNA expression. Cells were pretreated with GIE or DEX for 3 h and then coincubated with LPS plus IFN- γ for 24 h. UN: uninduced cells; IN: untreated LPS plus IFN- γ -induced cells; DEX: cells were pretreated with DEX at 1 μ M; GIE (50, 100, 200, and 300): cells were pretreated with GIE at concentrations range of 50, 100, 200, and 300 μ g/mL, respectively. The data represent the mean ± S.D. of two independent experiments. One-way ANOVA performed the comparison, and Tukey was used as a post hoc test. The degree of significance was denoted with different letters for the comparison between sample groups. p < 0.05 was considered as statistically significant.
    Figure Legend Snippet: GIE suppressed the secretion of (a) proinflammatory cytokines IL-6, (b) TNF- α , and slightly increased (c) anti-inflammatory cytokines IL-10 secretion in LPS plus IFN- γ -induced RAW264.7 cells. The effects of GIE on (d) IL-6 and (e) TNF- α mRNA expression. Cells were pretreated with GIE or DEX for 3 h and then coincubated with LPS plus IFN- γ for 24 h. UN: uninduced cells; IN: untreated LPS plus IFN- γ -induced cells; DEX: cells were pretreated with DEX at 1 μ M; GIE (50, 100, 200, and 300): cells were pretreated with GIE at concentrations range of 50, 100, 200, and 300 μ g/mL, respectively. The data represent the mean ± S.D. of two independent experiments. One-way ANOVA performed the comparison, and Tukey was used as a post hoc test. The degree of significance was denoted with different letters for the comparison between sample groups. p < 0.05 was considered as statistically significant.

    Techniques Used: Expressing

    Effects of GIE on the morphology of LPS plus IFN- γ -induced RAW264.7 cells. Cells were stained with hematoxylin staining: (a) uninduced RAW264.7 cells, (b) untreated LPS plus IFN- γ -induced cells, (c) cells were pretreated with DEX at 1 μ M, (d), (e), (f), and (g) cells were pretreated with GIE at concentrations range of 50, 100, 200, and 300 μ g/mL, respectively (original magnification at ×600, scale bar; 10 μ m).
    Figure Legend Snippet: Effects of GIE on the morphology of LPS plus IFN- γ -induced RAW264.7 cells. Cells were stained with hematoxylin staining: (a) uninduced RAW264.7 cells, (b) untreated LPS plus IFN- γ -induced cells, (c) cells were pretreated with DEX at 1 μ M, (d), (e), (f), and (g) cells were pretreated with GIE at concentrations range of 50, 100, 200, and 300 μ g/mL, respectively (original magnification at ×600, scale bar; 10 μ m).

    Techniques Used: Staining

    Effects of GIE on the nuclear translocation of NF- κ B p65 in LPS plus IFN- γ -induced RAW264.7 cells at 24 h. Cells were pretreated with GIE or DEX for 3 h and then coincubated with LPS plus IFN- γ for 24 h. The nuclear translocation of NF- κ B p65 was detected using an immunofluorescence assay and visualized under confocal microscopy. The figure represents the cell morphology (bright field), the nuclear translocation of NF- κ B p65 (green fluorescence), nucleus (blue fluorescence), and costaining (overlay green and blue fluorescence). Scale bar, 20 μ m. UN: uninduced cells; IN: untreated LPS plus IFN- γ -induced cells; DEX: cells were pretreated with DEX at 1 μ M; GIE300: cells were pretreated with GIE 300 μ g/mL.
    Figure Legend Snippet: Effects of GIE on the nuclear translocation of NF- κ B p65 in LPS plus IFN- γ -induced RAW264.7 cells at 24 h. Cells were pretreated with GIE or DEX for 3 h and then coincubated with LPS plus IFN- γ for 24 h. The nuclear translocation of NF- κ B p65 was detected using an immunofluorescence assay and visualized under confocal microscopy. The figure represents the cell morphology (bright field), the nuclear translocation of NF- κ B p65 (green fluorescence), nucleus (blue fluorescence), and costaining (overlay green and blue fluorescence). Scale bar, 20 μ m. UN: uninduced cells; IN: untreated LPS plus IFN- γ -induced cells; DEX: cells were pretreated with DEX at 1 μ M; GIE300: cells were pretreated with GIE 300 μ g/mL.

    Techniques Used: Translocation Assay, Immunofluorescence, Confocal Microscopy, Fluorescence

    Effects of GIE on phosphorylation of NF- κ B p65 induced by LPS plus IFN- γ in RAW264.7 cells. Cells were pretreated with GIE or DEX for 3 h and then coincubated with LPS plus IFN- γ for 24 h. The protein expression was analyzed by Western blotting. (a) The cellular proteins were used to detect the phosphorylated NF- κ B p65 and total form of NF- κ B with α -tubulin as a housekeeping control protein. (b) Mean densitometric values are expressed as bar charts. The data represent the mean ± S.D. of three independent experiments. One-way ANOVA performed the comparison, and Tukey was used as a post hoc test. The degree of significance was denoted with different letters for the comparison between sample groups. p < 0.05 was considered as statistically significant. UN: uninduced cells; IN: untreated LPS plus IFN- γ -induced cells; DEX: cells were pretreated with DEX at 1 μ M; GIE300: cells were pretreated with GIE 300 μ g/mL.
    Figure Legend Snippet: Effects of GIE on phosphorylation of NF- κ B p65 induced by LPS plus IFN- γ in RAW264.7 cells. Cells were pretreated with GIE or DEX for 3 h and then coincubated with LPS plus IFN- γ for 24 h. The protein expression was analyzed by Western blotting. (a) The cellular proteins were used to detect the phosphorylated NF- κ B p65 and total form of NF- κ B with α -tubulin as a housekeeping control protein. (b) Mean densitometric values are expressed as bar charts. The data represent the mean ± S.D. of three independent experiments. One-way ANOVA performed the comparison, and Tukey was used as a post hoc test. The degree of significance was denoted with different letters for the comparison between sample groups. p < 0.05 was considered as statistically significant. UN: uninduced cells; IN: untreated LPS plus IFN- γ -induced cells; DEX: cells were pretreated with DEX at 1 μ M; GIE300: cells were pretreated with GIE 300 μ g/mL.

    Techniques Used: Expressing, Western Blot

    (a) Cytotoxic effects of Vit.E on RAW264.7 cells for 24 h. MTT assay was used to determine cell viability. Values are expressed as a percentage of the control. (b) The effect of Vit.E compared to GIE on NO production in LPS plus IFN- γ -induced RAW264.7 cells. Nitrite concentration was determined from a sodium nitrite standard curve, and the results are expressed as a concentration ( μ M) of nitrite in a culture medium. The data represent the mean ± S.D. of three independent experiments. (c) The effects of Vit.E on COX-2 and iNOS mRNA expression in LPS plus IFN- γ -induced RAW264.7 cells. (d) The effects of Vit.E on TNF- α , IL-6, and SOD2 mRNA expression in LPS plus IFN- γ -induced RAW264.7 cells. The data represent the mean ± S.D. of two independent experiments. Cells were pretreated with GIE or DEX for 3 h and then coincubated with LPS plus IFN- γ for 24 h. UN: uninduced cells; IN: untreated LPS plus IFN- γ -induced cells; DEX: cells were pretreated with DEX at 1 μ M; GIE 300: cells were pretreated with GIE 300 μ g/mL; Vit.E (150 and 300): cells were pretreated with Vit.E 150 and 300 μ g/mL, respectively. One-way ANOVA performed the comparison, and Tukey was used as a post hoc test. The degree of significance was denoted with different letters for the comparison between sample groups. p < 0.05 was considered as statistically significant.
    Figure Legend Snippet: (a) Cytotoxic effects of Vit.E on RAW264.7 cells for 24 h. MTT assay was used to determine cell viability. Values are expressed as a percentage of the control. (b) The effect of Vit.E compared to GIE on NO production in LPS plus IFN- γ -induced RAW264.7 cells. Nitrite concentration was determined from a sodium nitrite standard curve, and the results are expressed as a concentration ( μ M) of nitrite in a culture medium. The data represent the mean ± S.D. of three independent experiments. (c) The effects of Vit.E on COX-2 and iNOS mRNA expression in LPS plus IFN- γ -induced RAW264.7 cells. (d) The effects of Vit.E on TNF- α , IL-6, and SOD2 mRNA expression in LPS plus IFN- γ -induced RAW264.7 cells. The data represent the mean ± S.D. of two independent experiments. Cells were pretreated with GIE or DEX for 3 h and then coincubated with LPS plus IFN- γ for 24 h. UN: uninduced cells; IN: untreated LPS plus IFN- γ -induced cells; DEX: cells were pretreated with DEX at 1 μ M; GIE 300: cells were pretreated with GIE 300 μ g/mL; Vit.E (150 and 300): cells were pretreated with Vit.E 150 and 300 μ g/mL, respectively. One-way ANOVA performed the comparison, and Tukey was used as a post hoc test. The degree of significance was denoted with different letters for the comparison between sample groups. p < 0.05 was considered as statistically significant.

    Techniques Used: MTT Assay, Concentration Assay, Expressing

    raw264 7 cells  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH raw264 7 cells
    Invasion of epithelial HeLa cells ( A ) and replication within <t>RAW264.7</t> macrophages ( B ) by wild-type S. Typhimurium LSP 146/02, its Δ fetMP - flsDA isogenic derivative, and the latter strain complemented with the cloned fetMP - flsDA genes (LSP 146/02 Δ fetMP - flsDA C ). The data shown are the mean values ± standard deviations of three biological replicates. Statistical significance (**** p < 0.0001; *** p < 0.001; ** p < 0.01; * p < 0.05; n.s., not significant) was determined by one-way ANOVA and the Tukey´s post-test.
    Raw264 7 Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "A Plasmid-Encoded FetMP-Fls Iron Uptake System Confers Selective Advantages to Salmonella enterica Serovar Typhimurium in Growth under Iron-Restricted Conditions and for Infection of Mammalian Host Cells"

    Article Title: A Plasmid-Encoded FetMP-Fls Iron Uptake System Confers Selective Advantages to Salmonella enterica Serovar Typhimurium in Growth under Iron-Restricted Conditions and for Infection of Mammalian Host Cells

    Journal: Microorganisms

    doi: 10.3390/microorganisms8050630

    Invasion of epithelial HeLa cells ( A ) and replication within RAW264.7 macrophages ( B ) by wild-type S. Typhimurium LSP 146/02, its Δ fetMP - flsDA isogenic derivative, and the latter strain complemented with the cloned fetMP - flsDA genes (LSP 146/02 Δ fetMP - flsDA C ). The data shown are the mean values ± standard deviations of three biological replicates. Statistical significance (**** p < 0.0001; *** p < 0.001; ** p < 0.01; * p < 0.05; n.s., not significant) was determined by one-way ANOVA and the Tukey´s post-test.
    Figure Legend Snippet: Invasion of epithelial HeLa cells ( A ) and replication within RAW264.7 macrophages ( B ) by wild-type S. Typhimurium LSP 146/02, its Δ fetMP - flsDA isogenic derivative, and the latter strain complemented with the cloned fetMP - flsDA genes (LSP 146/02 Δ fetMP - flsDA C ). The data shown are the mean values ± standard deviations of three biological replicates. Statistical significance (**** p < 0.0001; *** p < 0.001; ** p < 0.01; * p < 0.05; n.s., not significant) was determined by one-way ANOVA and the Tukey´s post-test.

    Techniques Used: Clone Assay

    Invasion of epithelial HeLa cells by S. Typhimurium ATCC 14028 harboring the cloned fetMP - flsDA genes (ATCC 14028 fetMP - flsDA T ) ( A ) and replication of the same strain within RAW264.7 macrophages ( B ). The data are the mean values ± standard deviations of three biological replicates. Statistical significance (**** p < 0.0001; *** p < 0.001; ** p < 0.01) was determined by one-way ANOVA and the Tukey´s post-test.
    Figure Legend Snippet: Invasion of epithelial HeLa cells by S. Typhimurium ATCC 14028 harboring the cloned fetMP - flsDA genes (ATCC 14028 fetMP - flsDA T ) ( A ) and replication of the same strain within RAW264.7 macrophages ( B ). The data are the mean values ± standard deviations of three biological replicates. Statistical significance (**** p < 0.0001; *** p < 0.001; ** p < 0.01) was determined by one-way ANOVA and the Tukey´s post-test.

    Techniques Used: Clone Assay

    raw264 7 cells  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH raw264 7 cells
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    raw264 7 macrophage cells  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH raw264 7 macrophage cells
    Effects of O. indicum on cell viability in <t>RAW264.7</t> cells. Cells were treated with different concentrations of O. indicum for 24 h. Cell viability was determined by the MTT assay. CON: cells without O. indicum ; 50–1,000: cells were treated with O. indicum at the concentration range of 50–1,000 μ g mL −1 . Values are expressed as a percentage of the control. The data represent the mean ± SD of three independent experiments. Bars marked with different letters are significantly different at p < 0.05 as determined by one-way ANOVA with Tukey's post hoc test.
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    1) Product Images from "Intracellular ROS Scavenging and Anti-Inflammatory Activities of Oroxylum indicum Kurz (L.) Extract in LPS plus IFN- γ -Activated RAW264.7 Macrophages"

    Article Title: Intracellular ROS Scavenging and Anti-Inflammatory Activities of Oroxylum indicum Kurz (L.) Extract in LPS plus IFN- γ -Activated RAW264.7 Macrophages

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    doi: 10.1155/2020/7436920

    Effects of O. indicum on cell viability in RAW264.7 cells. Cells were treated with different concentrations of O. indicum for 24 h. Cell viability was determined by the MTT assay. CON: cells without O. indicum ; 50–1,000: cells were treated with O. indicum at the concentration range of 50–1,000 μ g mL −1 . Values are expressed as a percentage of the control. The data represent the mean ± SD of three independent experiments. Bars marked with different letters are significantly different at p < 0.05 as determined by one-way ANOVA with Tukey's post hoc test.
    Figure Legend Snippet: Effects of O. indicum on cell viability in RAW264.7 cells. Cells were treated with different concentrations of O. indicum for 24 h. Cell viability was determined by the MTT assay. CON: cells without O. indicum ; 50–1,000: cells were treated with O. indicum at the concentration range of 50–1,000 μ g mL −1 . Values are expressed as a percentage of the control. The data represent the mean ± SD of three independent experiments. Bars marked with different letters are significantly different at p < 0.05 as determined by one-way ANOVA with Tukey's post hoc test.

    Techniques Used: MTT Assay, Concentration Assay

    Effects of O. indicum on the intracellular ROS production in LPS plus IFN- γ -activated RAW264.7 cells. Cells were pretreated with different concentrations of O. indicum for 3 h and then activated with LPS plus IFN- γ for 24 h. Cell viability and ROS intensity are expressed as a percentage of the control. UNA: unactivated cells; CON: cells without O. indicum ; NAC: cells were pretreated with NAC at 3 mM; VIT.C: cells were pretreated with VIT.C at 50 μ g mL −1 ; and 50, 100, and 200: cells were pretreated with O. indicum at 50, 100, and 200 μ g mL −1 , respectively. Values are expressed as a percentage of the control. The data represent the mean ± SD of three independent experiments. Bars marked with different letters are significantly different at p < 0.05 as determined by one-way ANOVA with Tukey's post hoc test.
    Figure Legend Snippet: Effects of O. indicum on the intracellular ROS production in LPS plus IFN- γ -activated RAW264.7 cells. Cells were pretreated with different concentrations of O. indicum for 3 h and then activated with LPS plus IFN- γ for 24 h. Cell viability and ROS intensity are expressed as a percentage of the control. UNA: unactivated cells; CON: cells without O. indicum ; NAC: cells were pretreated with NAC at 3 mM; VIT.C: cells were pretreated with VIT.C at 50 μ g mL −1 ; and 50, 100, and 200: cells were pretreated with O. indicum at 50, 100, and 200 μ g mL −1 , respectively. Values are expressed as a percentage of the control. The data represent the mean ± SD of three independent experiments. Bars marked with different letters are significantly different at p < 0.05 as determined by one-way ANOVA with Tukey's post hoc test.

    Techniques Used:

    Effects of O. indicum on (a) NO production, (b) proinflammatory cytokines IL-6, and (c) TNF- α secretion in LPS plus IFN- γ -activated RAW264.7 cells. Cells were pretreated with different concentrations of O. indicum for 3 h and then activated with LPS plus IFN- γ for 24 h UNA: unactivated cells; CON: cells without O. indicum ; DEX: cells were pretreated with DEX at 1 μ M; 50, 100, and 200: cells were pretreated with O. indicum at 50, 100, and 200 μ g mL −1 , respectively. The data represent the mean ± SD of three independent experiments. Bars marked with different letters are significantly different at p < 0.05 as determined by one-way ANOVA with Tukey's post hoc test.
    Figure Legend Snippet: Effects of O. indicum on (a) NO production, (b) proinflammatory cytokines IL-6, and (c) TNF- α secretion in LPS plus IFN- γ -activated RAW264.7 cells. Cells were pretreated with different concentrations of O. indicum for 3 h and then activated with LPS plus IFN- γ for 24 h UNA: unactivated cells; CON: cells without O. indicum ; DEX: cells were pretreated with DEX at 1 μ M; 50, 100, and 200: cells were pretreated with O. indicum at 50, 100, and 200 μ g mL −1 , respectively. The data represent the mean ± SD of three independent experiments. Bars marked with different letters are significantly different at p < 0.05 as determined by one-way ANOVA with Tukey's post hoc test.

    Techniques Used:

    Effects of O. indicum on the morphology of LPS plus IFN- γ -activated RAW264.7 cells. Haematoxylin staining of 6 different groups of the sample. a: unactivated RAW264.7 cells; b: activated RAW264.7 cells (cells without O. indicum ); c: cells were pretreated with DEX at 1 μ M; d, e, and f: cells were pretreated with O. indicum at 50, 100, and 200 μ g mL −1 , respectively (original magnification at ×400, scale bar; 20 μ m).
    Figure Legend Snippet: Effects of O. indicum on the morphology of LPS plus IFN- γ -activated RAW264.7 cells. Haematoxylin staining of 6 different groups of the sample. a: unactivated RAW264.7 cells; b: activated RAW264.7 cells (cells without O. indicum ); c: cells were pretreated with DEX at 1 μ M; d, e, and f: cells were pretreated with O. indicum at 50, 100, and 200 μ g mL −1 , respectively (original magnification at ×400, scale bar; 20 μ m).

    Techniques Used: Staining

    Effects of O. indicum on biomolecular changes detected by FTIR. (a) Average original FTIR spectra (3500–950 cm −1 ). (b) Average the secondary derivative spectra of lipid regions (3000–2800 cm −1 ) and protein regions (1800–1400 cm −1 ). (c) The bar graph of integrated areas of lipid regions (3000–2800 cm −1 ) and protein regions (1800–1400 cm −1 ). The data obtained from unactivated RAW264.7 cells ( n = 120), activated RAW264.7 (LPS plus IFN- γ ) ( n = 120), and activated RAW264.7 (LPS plus IFN- γ ) exposed to 1 μ M DEX ( n = 157) or 200 μ g mL −1 of O. indicum ( n = 135). Data are represented as means ± SD for three replicates. Bars marked with different letters are significantly different at p < 0.05 as determined by one-way ANOVA with Tukey's post hoc test.
    Figure Legend Snippet: Effects of O. indicum on biomolecular changes detected by FTIR. (a) Average original FTIR spectra (3500–950 cm −1 ). (b) Average the secondary derivative spectra of lipid regions (3000–2800 cm −1 ) and protein regions (1800–1400 cm −1 ). (c) The bar graph of integrated areas of lipid regions (3000–2800 cm −1 ) and protein regions (1800–1400 cm −1 ). The data obtained from unactivated RAW264.7 cells ( n = 120), activated RAW264.7 (LPS plus IFN- γ ) ( n = 120), and activated RAW264.7 (LPS plus IFN- γ ) exposed to 1 μ M DEX ( n = 157) or 200 μ g mL −1 of O. indicum ( n = 135). Data are represented as means ± SD for three replicates. Bars marked with different letters are significantly different at p < 0.05 as determined by one-way ANOVA with Tukey's post hoc test.

    Techniques Used:

    PCA analysis of FTIR spectral range 3000–2800 cm −1 and 1800–1400 cm −1 giving (a) PCA 3D score plot and (b) PCA loading plot. PCA score plots showed distinct clustering between unactivated RAW264.7 cells ( n = 120), activated RAW264.7 (LPS plus IFN- γ ) ( n = 120), and activated RAW264.7 (LPS plus IFN- γ ) exposed to 1 μ M DEX ( n = 157) or 200 μ g mL −1 of O. indicum ( n = 135). PCA loading plots identify biomarker differences over a spectral range of samples.
    Figure Legend Snippet: PCA analysis of FTIR spectral range 3000–2800 cm −1 and 1800–1400 cm −1 giving (a) PCA 3D score plot and (b) PCA loading plot. PCA score plots showed distinct clustering between unactivated RAW264.7 cells ( n = 120), activated RAW264.7 (LPS plus IFN- γ ) ( n = 120), and activated RAW264.7 (LPS plus IFN- γ ) exposed to 1 μ M DEX ( n = 157) or 200 μ g mL −1 of O. indicum ( n = 135). PCA loading plots identify biomarker differences over a spectral range of samples.

    Techniques Used: Biomarker Assay

    raw264 7 macrophages  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH raw264 7 macrophages
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    CLS Cell Lines Service GmbH sirna transfection raw264 7 macrophages
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    CLS Cell Lines Service GmbH raw264 7 cells
    Intracellular reactive oxygen species (ROS)-scavenging effect of G-TSrP and release profile of TA and Sr 2+ . 2′,7′-dichlorofluorescein diacetate (DCFDA) assay images and their quantification using (a) HDFBs (scale bar: 100 μm) and (b) <t>RAW264.7</t> cells (scale bar: 50 μm) treated with different hydrogel extracts and H 2 O 2 . (c) DCFDA assay images and their quantification using RAW264.7 cells after treatment of hydrogel extracts and lipopolysaccharide (LPS). Scale bar: 50 μm. (d) Release profile of TA and Sr 2+ from G-TSrP for 14 days.
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    CLS Cell Lines Service GmbH raw264 7 murine macrophage cells
    Intracellular reactive oxygen species (ROS)-scavenging effect of G-TSrP and release profile of TA and Sr 2+ . 2′,7′-dichlorofluorescein diacetate (DCFDA) assay images and their quantification using (a) HDFBs (scale bar: 100 μm) and (b) <t>RAW264.7</t> cells (scale bar: 50 μm) treated with different hydrogel extracts and H 2 O 2 . (c) DCFDA assay images and their quantification using RAW264.7 cells after treatment of hydrogel extracts and lipopolysaccharide (LPS). Scale bar: 50 μm. (d) Release profile of TA and Sr 2+ from G-TSrP for 14 days.
    Raw264 7 Murine Macrophage Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    CLS Cell Lines Service GmbH raw264 7 macrophages
    Gene expression of Acp-5 ( a ), release of TRAP ( b ) and CaP resorption ( c ) with normal salt (NS) and high salt treatment (HS) in murine osteoclasts differentiated from <t>RAW264.7</t> macrophages (n ≥ 6). Differentiation of osteoclasts was induced by treatment with M-CSF (30 ng/mL) and RANKL (50 ng/mL) for five days, followed by addition of 40 mM NaCl to the HS group. Symbols represent single data points, horizontal lines the arithmetic mean and vertical lines the standard error of the mean. AU: arbitrary units. Statistics: Two-tailed unpaired t-test. ** p < 0.01; *** p < 0.001.
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    CLS Cell Lines Service GmbH murine macrophage cell line raw264 7
    (a) Cytotoxic effects of GIE on <t>RAW264.7</t> cells. Cells were treated with different concentrations of GIE (100-500 μ g/mL) for 24 h. MTT assay was used to determine cell viability. Values are expressed as a percentage of the control. (b) GIE attenuated the intracellular ROS production in LPS plus IFN- γ -induced RAW264.7 cells. The intracellular ROS levels are expressed as a percentage of the control. (c) The effects of GIE on antioxidant mRNA expression in LPS plus IFN- γ -induced RAW264.7 cells. (d) GIE suppressed NO production in LPS plus IFN- γ -induced RAW264.7 cells. Nitrite concentration was determined from a sodium nitrite standard curve, and the results are expressed as a concentration ( μ M) of nitrite in a culture medium. (e) The effects of GIE on COX-2 and iNOS mRNA expression in LPS plus IFN- γ -induced RAW264.7 cells. The data represent the mean ± S.D. of two independent experiments. Cells were pretreated with GIE, NAC, or DEX for 3 h and then coincubated with LPS plus IFN- γ for 24 h. UN: uninduced cells; IN: untreated LPS plus IFN- γ -induced cells; NAC: cells were pretreated with NAC at 3 mM; DEX: cells were pretreated with DEX at 1 μ M; GIE (50, 100, 200, and 300): cells were pretreated with GIE at concentrations range of 50, 100, 200, and 300 μ g/mL, respectively. One-way ANOVA performed the comparison, and Tukey was used as a post hoc test. The degree of significance was denoted with different letters for the comparison between sample groups. p < 0.05 was considered as statistically significant.
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    CLS Cell Lines Service GmbH raw264 7 macrophage cells
    Effects of O. indicum on cell viability in <t>RAW264.7</t> cells. Cells were treated with different concentrations of O. indicum for 24 h. Cell viability was determined by the MTT assay. CON: cells without O. indicum ; 50–1,000: cells were treated with O. indicum at the concentration range of 50–1,000 μ g mL −1 . Values are expressed as a percentage of the control. The data represent the mean ± SD of three independent experiments. Bars marked with different letters are significantly different at p < 0.05 as determined by one-way ANOVA with Tukey's post hoc test.
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    Image Search Results


    Intracellular reactive oxygen species (ROS)-scavenging effect of G-TSrP and release profile of TA and Sr 2+ . 2′,7′-dichlorofluorescein diacetate (DCFDA) assay images and their quantification using (a) HDFBs (scale bar: 100 μm) and (b) RAW264.7 cells (scale bar: 50 μm) treated with different hydrogel extracts and H 2 O 2 . (c) DCFDA assay images and their quantification using RAW264.7 cells after treatment of hydrogel extracts and lipopolysaccharide (LPS). Scale bar: 50 μm. (d) Release profile of TA and Sr 2+ from G-TSrP for 14 days.

    Journal: Bioactive Materials

    Article Title: Cell-homing and immunomodulatory composite hydrogels for effective wound healing with neovascularization

    doi: 10.1016/j.bioactmat.2024.02.029

    Figure Lengend Snippet: Intracellular reactive oxygen species (ROS)-scavenging effect of G-TSrP and release profile of TA and Sr 2+ . 2′,7′-dichlorofluorescein diacetate (DCFDA) assay images and their quantification using (a) HDFBs (scale bar: 100 μm) and (b) RAW264.7 cells (scale bar: 50 μm) treated with different hydrogel extracts and H 2 O 2 . (c) DCFDA assay images and their quantification using RAW264.7 cells after treatment of hydrogel extracts and lipopolysaccharide (LPS). Scale bar: 50 μm. (d) Release profile of TA and Sr 2+ from G-TSrP for 14 days.

    Article Snippet: HaCaT cells were acquired from Cell Lines Service (Eppelheim, Germany), and RAW264.7 cells were purchased from the Korean Cell Line Bank (Seoul, Korea).

    Techniques:

    Modulation of macrophage polarization by G-TSrP. (a) Percentage of iNOS-positive cells from immunofluorescence (IF) images of RAW264.7 cells after treatment with LPS and hydrogel extracts. (b) F-actin staining images of RAW264.7 cells after treatment with LPS and hydrogel extracts, along with measurement of their cell area. Scale bar: 25 μm. (c) Percentage of CD206-positive cells from IF images of RAW264.7 cells after treatment with interleukin (IL)-4 and hydrogel extracts. (d) F-actin staining images of RAW264.7 cells after treatment with IL-4 and hydrogel extracts, along with measurement of their aspect ratio. Scale bar: 25 μm. Relative gene expression of (e) M1 polarization markers and (f) M2 polarization markers of RAW264.7 cells cultured using hydrogel extracts with their induction factors (M1: LPS, M2: IL-4).

    Journal: Bioactive Materials

    Article Title: Cell-homing and immunomodulatory composite hydrogels for effective wound healing with neovascularization

    doi: 10.1016/j.bioactmat.2024.02.029

    Figure Lengend Snippet: Modulation of macrophage polarization by G-TSrP. (a) Percentage of iNOS-positive cells from immunofluorescence (IF) images of RAW264.7 cells after treatment with LPS and hydrogel extracts. (b) F-actin staining images of RAW264.7 cells after treatment with LPS and hydrogel extracts, along with measurement of their cell area. Scale bar: 25 μm. (c) Percentage of CD206-positive cells from IF images of RAW264.7 cells after treatment with interleukin (IL)-4 and hydrogel extracts. (d) F-actin staining images of RAW264.7 cells after treatment with IL-4 and hydrogel extracts, along with measurement of their aspect ratio. Scale bar: 25 μm. Relative gene expression of (e) M1 polarization markers and (f) M2 polarization markers of RAW264.7 cells cultured using hydrogel extracts with their induction factors (M1: LPS, M2: IL-4).

    Article Snippet: HaCaT cells were acquired from Cell Lines Service (Eppelheim, Germany), and RAW264.7 cells were purchased from the Korean Cell Line Bank (Seoul, Korea).

    Techniques: Immunofluorescence, Staining, Expressing, Cell Culture

    In vivo and in vitro cell homing and biodegradation of composite hydrogels. (a) Hematoxylin and eosin (H&E) staining images (scale bar: 250 μm) and their magnified images (scale bar: 50 μm) of mouse subcutaneous tissues 14 days after hydrogel implantation. (b) Representative dual IF staining (F4/80 and iNOS) images (scale bar: 250 μm) and their magnified images (scale bar: 50 μm) of mouse subcutaneous tissues 14 days after implantation, along with their quantification. (c) Representative dual IF staining (F4/80 and CD206) images (scale bar: 250 μm) and their magnified images (scale bar: 50 μm) of mouse subcutaneous tissues 14 days after implantation, along with their quantification. (d) Images of migrated RAW264.7 cells cultured with GelMA and G-TSrP hydrogel for 24 h and their quantification. Scale bar: 250 μm. (e) Relative gene expression of MMP9 and MMP13 of RAW264.7 cells cultured with IL-4 and each hydrogel extract.

    Journal: Bioactive Materials

    Article Title: Cell-homing and immunomodulatory composite hydrogels for effective wound healing with neovascularization

    doi: 10.1016/j.bioactmat.2024.02.029

    Figure Lengend Snippet: In vivo and in vitro cell homing and biodegradation of composite hydrogels. (a) Hematoxylin and eosin (H&E) staining images (scale bar: 250 μm) and their magnified images (scale bar: 50 μm) of mouse subcutaneous tissues 14 days after hydrogel implantation. (b) Representative dual IF staining (F4/80 and iNOS) images (scale bar: 250 μm) and their magnified images (scale bar: 50 μm) of mouse subcutaneous tissues 14 days after implantation, along with their quantification. (c) Representative dual IF staining (F4/80 and CD206) images (scale bar: 250 μm) and their magnified images (scale bar: 50 μm) of mouse subcutaneous tissues 14 days after implantation, along with their quantification. (d) Images of migrated RAW264.7 cells cultured with GelMA and G-TSrP hydrogel for 24 h and their quantification. Scale bar: 250 μm. (e) Relative gene expression of MMP9 and MMP13 of RAW264.7 cells cultured with IL-4 and each hydrogel extract.

    Article Snippet: HaCaT cells were acquired from Cell Lines Service (Eppelheim, Germany), and RAW264.7 cells were purchased from the Korean Cell Line Bank (Seoul, Korea).

    Techniques: In Vivo, In Vitro, Staining, Cell Culture, Expressing

    Genetic analysis of macrophage-mediated inflammatory wound healing. (a) Total heatmap data of RAW264.7 cells after treatment of different hydrogel extracts in the presence of LPS. (b) Volcano plots representing gene expression in the G-TSP group compared with the GelMA group. (c) Principal component analysis (PCA) plot displaying the variances of the genes in macrophages of GelMA, TA and G-TSrP group. (d) Up-regulated and down-regulated gene ontology (GO) analysis. (e) Schematic illustration of the mechanism of macrophage-mediated wound healing process promoted by G-TSrP. GO enrichment analysis of (f) positive regulation of immune response, (g) cell migration, and (h) angiogenesis.

    Journal: Bioactive Materials

    Article Title: Cell-homing and immunomodulatory composite hydrogels for effective wound healing with neovascularization

    doi: 10.1016/j.bioactmat.2024.02.029

    Figure Lengend Snippet: Genetic analysis of macrophage-mediated inflammatory wound healing. (a) Total heatmap data of RAW264.7 cells after treatment of different hydrogel extracts in the presence of LPS. (b) Volcano plots representing gene expression in the G-TSP group compared with the GelMA group. (c) Principal component analysis (PCA) plot displaying the variances of the genes in macrophages of GelMA, TA and G-TSrP group. (d) Up-regulated and down-regulated gene ontology (GO) analysis. (e) Schematic illustration of the mechanism of macrophage-mediated wound healing process promoted by G-TSrP. GO enrichment analysis of (f) positive regulation of immune response, (g) cell migration, and (h) angiogenesis.

    Article Snippet: HaCaT cells were acquired from Cell Lines Service (Eppelheim, Germany), and RAW264.7 cells were purchased from the Korean Cell Line Bank (Seoul, Korea).

    Techniques: Expressing, Migration

    Gene expression of Acp-5 ( a ), release of TRAP ( b ) and CaP resorption ( c ) with normal salt (NS) and high salt treatment (HS) in murine osteoclasts differentiated from RAW264.7 macrophages (n ≥ 6). Differentiation of osteoclasts was induced by treatment with M-CSF (30 ng/mL) and RANKL (50 ng/mL) for five days, followed by addition of 40 mM NaCl to the HS group. Symbols represent single data points, horizontal lines the arithmetic mean and vertical lines the standard error of the mean. AU: arbitrary units. Statistics: Two-tailed unpaired t-test. ** p < 0.01; *** p < 0.001.

    Journal: International Journal of Molecular Sciences

    Article Title: Dietary Salt Accelerates Orthodontic Tooth Movement by Increased Osteoclast Activity

    doi: 10.3390/ijms22020596

    Figure Lengend Snippet: Gene expression of Acp-5 ( a ), release of TRAP ( b ) and CaP resorption ( c ) with normal salt (NS) and high salt treatment (HS) in murine osteoclasts differentiated from RAW264.7 macrophages (n ≥ 6). Differentiation of osteoclasts was induced by treatment with M-CSF (30 ng/mL) and RANKL (50 ng/mL) for five days, followed by addition of 40 mM NaCl to the HS group. Symbols represent single data points, horizontal lines the arithmetic mean and vertical lines the standard error of the mean. AU: arbitrary units. Statistics: Two-tailed unpaired t-test. ** p < 0.01; *** p < 0.001.

    Article Snippet: Experiment 1: We cultured 10,000 RAW264.7 macrophages (400319, Cell Lines Service, Eppelheim, Germany) in 1 mL α-MEM (F0925, Biochrom, Berlin, Germany) supplemented with 10% fetal bovine serum (P30-3306, PAN-Biotech, Aidenbach, Germany), 1% L-glutamine (SH30034.01, GE-Healthcare, Chigaco, IL, USA) and 1% antibiotics/antimycotics (A5955, Sigma Aldrich, St. Louis, MO, USA).

    Techniques: Expressing, Two Tailed Test

    (a) Cytotoxic effects of GIE on RAW264.7 cells. Cells were treated with different concentrations of GIE (100-500 μ g/mL) for 24 h. MTT assay was used to determine cell viability. Values are expressed as a percentage of the control. (b) GIE attenuated the intracellular ROS production in LPS plus IFN- γ -induced RAW264.7 cells. The intracellular ROS levels are expressed as a percentage of the control. (c) The effects of GIE on antioxidant mRNA expression in LPS plus IFN- γ -induced RAW264.7 cells. (d) GIE suppressed NO production in LPS plus IFN- γ -induced RAW264.7 cells. Nitrite concentration was determined from a sodium nitrite standard curve, and the results are expressed as a concentration ( μ M) of nitrite in a culture medium. (e) The effects of GIE on COX-2 and iNOS mRNA expression in LPS plus IFN- γ -induced RAW264.7 cells. The data represent the mean ± S.D. of two independent experiments. Cells were pretreated with GIE, NAC, or DEX for 3 h and then coincubated with LPS plus IFN- γ for 24 h. UN: uninduced cells; IN: untreated LPS plus IFN- γ -induced cells; NAC: cells were pretreated with NAC at 3 mM; DEX: cells were pretreated with DEX at 1 μ M; GIE (50, 100, 200, and 300): cells were pretreated with GIE at concentrations range of 50, 100, 200, and 300 μ g/mL, respectively. One-way ANOVA performed the comparison, and Tukey was used as a post hoc test. The degree of significance was denoted with different letters for the comparison between sample groups. p < 0.05 was considered as statistically significant.

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Gymnema inodorum (Lour.) Decne. Extract Alleviates Oxidative Stress and Inflammatory Mediators Produced by RAW264.7 Macrophages

    doi: 10.1155/2021/8658314

    Figure Lengend Snippet: (a) Cytotoxic effects of GIE on RAW264.7 cells. Cells were treated with different concentrations of GIE (100-500 μ g/mL) for 24 h. MTT assay was used to determine cell viability. Values are expressed as a percentage of the control. (b) GIE attenuated the intracellular ROS production in LPS plus IFN- γ -induced RAW264.7 cells. The intracellular ROS levels are expressed as a percentage of the control. (c) The effects of GIE on antioxidant mRNA expression in LPS plus IFN- γ -induced RAW264.7 cells. (d) GIE suppressed NO production in LPS plus IFN- γ -induced RAW264.7 cells. Nitrite concentration was determined from a sodium nitrite standard curve, and the results are expressed as a concentration ( μ M) of nitrite in a culture medium. (e) The effects of GIE on COX-2 and iNOS mRNA expression in LPS plus IFN- γ -induced RAW264.7 cells. The data represent the mean ± S.D. of two independent experiments. Cells were pretreated with GIE, NAC, or DEX for 3 h and then coincubated with LPS plus IFN- γ for 24 h. UN: uninduced cells; IN: untreated LPS plus IFN- γ -induced cells; NAC: cells were pretreated with NAC at 3 mM; DEX: cells were pretreated with DEX at 1 μ M; GIE (50, 100, 200, and 300): cells were pretreated with GIE at concentrations range of 50, 100, 200, and 300 μ g/mL, respectively. One-way ANOVA performed the comparison, and Tukey was used as a post hoc test. The degree of significance was denoted with different letters for the comparison between sample groups. p < 0.05 was considered as statistically significant.

    Article Snippet: The murine macrophage cell line RAW264.7 (CLS Cell Lines Service GmbH., Eppelheim, Germany) was cultured in RPMI-1640 medium (Cat. No. 1IVG1-31800022) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Cat. No. 1IVG7-10270-106) and 100 U/mL penicillin-streptomycin (Cat. No. 1IVG7-15140-122) (GIBCO, Grand Island, NY, USA).

    Techniques: MTT Assay, Expressing, Concentration Assay

    GIE suppressed the secretion of (a) proinflammatory cytokines IL-6, (b) TNF- α , and slightly increased (c) anti-inflammatory cytokines IL-10 secretion in LPS plus IFN- γ -induced RAW264.7 cells. The effects of GIE on (d) IL-6 and (e) TNF- α mRNA expression. Cells were pretreated with GIE or DEX for 3 h and then coincubated with LPS plus IFN- γ for 24 h. UN: uninduced cells; IN: untreated LPS plus IFN- γ -induced cells; DEX: cells were pretreated with DEX at 1 μ M; GIE (50, 100, 200, and 300): cells were pretreated with GIE at concentrations range of 50, 100, 200, and 300 μ g/mL, respectively. The data represent the mean ± S.D. of two independent experiments. One-way ANOVA performed the comparison, and Tukey was used as a post hoc test. The degree of significance was denoted with different letters for the comparison between sample groups. p < 0.05 was considered as statistically significant.

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Gymnema inodorum (Lour.) Decne. Extract Alleviates Oxidative Stress and Inflammatory Mediators Produced by RAW264.7 Macrophages

    doi: 10.1155/2021/8658314

    Figure Lengend Snippet: GIE suppressed the secretion of (a) proinflammatory cytokines IL-6, (b) TNF- α , and slightly increased (c) anti-inflammatory cytokines IL-10 secretion in LPS plus IFN- γ -induced RAW264.7 cells. The effects of GIE on (d) IL-6 and (e) TNF- α mRNA expression. Cells were pretreated with GIE or DEX for 3 h and then coincubated with LPS plus IFN- γ for 24 h. UN: uninduced cells; IN: untreated LPS plus IFN- γ -induced cells; DEX: cells were pretreated with DEX at 1 μ M; GIE (50, 100, 200, and 300): cells were pretreated with GIE at concentrations range of 50, 100, 200, and 300 μ g/mL, respectively. The data represent the mean ± S.D. of two independent experiments. One-way ANOVA performed the comparison, and Tukey was used as a post hoc test. The degree of significance was denoted with different letters for the comparison between sample groups. p < 0.05 was considered as statistically significant.

    Article Snippet: The murine macrophage cell line RAW264.7 (CLS Cell Lines Service GmbH., Eppelheim, Germany) was cultured in RPMI-1640 medium (Cat. No. 1IVG1-31800022) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Cat. No. 1IVG7-10270-106) and 100 U/mL penicillin-streptomycin (Cat. No. 1IVG7-15140-122) (GIBCO, Grand Island, NY, USA).

    Techniques: Expressing

    Effects of GIE on the morphology of LPS plus IFN- γ -induced RAW264.7 cells. Cells were stained with hematoxylin staining: (a) uninduced RAW264.7 cells, (b) untreated LPS plus IFN- γ -induced cells, (c) cells were pretreated with DEX at 1 μ M, (d), (e), (f), and (g) cells were pretreated with GIE at concentrations range of 50, 100, 200, and 300 μ g/mL, respectively (original magnification at ×600, scale bar; 10 μ m).

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Gymnema inodorum (Lour.) Decne. Extract Alleviates Oxidative Stress and Inflammatory Mediators Produced by RAW264.7 Macrophages

    doi: 10.1155/2021/8658314

    Figure Lengend Snippet: Effects of GIE on the morphology of LPS plus IFN- γ -induced RAW264.7 cells. Cells were stained with hematoxylin staining: (a) uninduced RAW264.7 cells, (b) untreated LPS plus IFN- γ -induced cells, (c) cells were pretreated with DEX at 1 μ M, (d), (e), (f), and (g) cells were pretreated with GIE at concentrations range of 50, 100, 200, and 300 μ g/mL, respectively (original magnification at ×600, scale bar; 10 μ m).

    Article Snippet: The murine macrophage cell line RAW264.7 (CLS Cell Lines Service GmbH., Eppelheim, Germany) was cultured in RPMI-1640 medium (Cat. No. 1IVG1-31800022) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Cat. No. 1IVG7-10270-106) and 100 U/mL penicillin-streptomycin (Cat. No. 1IVG7-15140-122) (GIBCO, Grand Island, NY, USA).

    Techniques: Staining

    Effects of GIE on the nuclear translocation of NF- κ B p65 in LPS plus IFN- γ -induced RAW264.7 cells at 24 h. Cells were pretreated with GIE or DEX for 3 h and then coincubated with LPS plus IFN- γ for 24 h. The nuclear translocation of NF- κ B p65 was detected using an immunofluorescence assay and visualized under confocal microscopy. The figure represents the cell morphology (bright field), the nuclear translocation of NF- κ B p65 (green fluorescence), nucleus (blue fluorescence), and costaining (overlay green and blue fluorescence). Scale bar, 20 μ m. UN: uninduced cells; IN: untreated LPS plus IFN- γ -induced cells; DEX: cells were pretreated with DEX at 1 μ M; GIE300: cells were pretreated with GIE 300 μ g/mL.

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Gymnema inodorum (Lour.) Decne. Extract Alleviates Oxidative Stress and Inflammatory Mediators Produced by RAW264.7 Macrophages

    doi: 10.1155/2021/8658314

    Figure Lengend Snippet: Effects of GIE on the nuclear translocation of NF- κ B p65 in LPS plus IFN- γ -induced RAW264.7 cells at 24 h. Cells were pretreated with GIE or DEX for 3 h and then coincubated with LPS plus IFN- γ for 24 h. The nuclear translocation of NF- κ B p65 was detected using an immunofluorescence assay and visualized under confocal microscopy. The figure represents the cell morphology (bright field), the nuclear translocation of NF- κ B p65 (green fluorescence), nucleus (blue fluorescence), and costaining (overlay green and blue fluorescence). Scale bar, 20 μ m. UN: uninduced cells; IN: untreated LPS plus IFN- γ -induced cells; DEX: cells were pretreated with DEX at 1 μ M; GIE300: cells were pretreated with GIE 300 μ g/mL.

    Article Snippet: The murine macrophage cell line RAW264.7 (CLS Cell Lines Service GmbH., Eppelheim, Germany) was cultured in RPMI-1640 medium (Cat. No. 1IVG1-31800022) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Cat. No. 1IVG7-10270-106) and 100 U/mL penicillin-streptomycin (Cat. No. 1IVG7-15140-122) (GIBCO, Grand Island, NY, USA).

    Techniques: Translocation Assay, Immunofluorescence, Confocal Microscopy, Fluorescence

    Effects of GIE on phosphorylation of NF- κ B p65 induced by LPS plus IFN- γ in RAW264.7 cells. Cells were pretreated with GIE or DEX for 3 h and then coincubated with LPS plus IFN- γ for 24 h. The protein expression was analyzed by Western blotting. (a) The cellular proteins were used to detect the phosphorylated NF- κ B p65 and total form of NF- κ B with α -tubulin as a housekeeping control protein. (b) Mean densitometric values are expressed as bar charts. The data represent the mean ± S.D. of three independent experiments. One-way ANOVA performed the comparison, and Tukey was used as a post hoc test. The degree of significance was denoted with different letters for the comparison between sample groups. p < 0.05 was considered as statistically significant. UN: uninduced cells; IN: untreated LPS plus IFN- γ -induced cells; DEX: cells were pretreated with DEX at 1 μ M; GIE300: cells were pretreated with GIE 300 μ g/mL.

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Gymnema inodorum (Lour.) Decne. Extract Alleviates Oxidative Stress and Inflammatory Mediators Produced by RAW264.7 Macrophages

    doi: 10.1155/2021/8658314

    Figure Lengend Snippet: Effects of GIE on phosphorylation of NF- κ B p65 induced by LPS plus IFN- γ in RAW264.7 cells. Cells were pretreated with GIE or DEX for 3 h and then coincubated with LPS plus IFN- γ for 24 h. The protein expression was analyzed by Western blotting. (a) The cellular proteins were used to detect the phosphorylated NF- κ B p65 and total form of NF- κ B with α -tubulin as a housekeeping control protein. (b) Mean densitometric values are expressed as bar charts. The data represent the mean ± S.D. of three independent experiments. One-way ANOVA performed the comparison, and Tukey was used as a post hoc test. The degree of significance was denoted with different letters for the comparison between sample groups. p < 0.05 was considered as statistically significant. UN: uninduced cells; IN: untreated LPS plus IFN- γ -induced cells; DEX: cells were pretreated with DEX at 1 μ M; GIE300: cells were pretreated with GIE 300 μ g/mL.

    Article Snippet: The murine macrophage cell line RAW264.7 (CLS Cell Lines Service GmbH., Eppelheim, Germany) was cultured in RPMI-1640 medium (Cat. No. 1IVG1-31800022) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Cat. No. 1IVG7-10270-106) and 100 U/mL penicillin-streptomycin (Cat. No. 1IVG7-15140-122) (GIBCO, Grand Island, NY, USA).

    Techniques: Expressing, Western Blot

    (a) Cytotoxic effects of Vit.E on RAW264.7 cells for 24 h. MTT assay was used to determine cell viability. Values are expressed as a percentage of the control. (b) The effect of Vit.E compared to GIE on NO production in LPS plus IFN- γ -induced RAW264.7 cells. Nitrite concentration was determined from a sodium nitrite standard curve, and the results are expressed as a concentration ( μ M) of nitrite in a culture medium. The data represent the mean ± S.D. of three independent experiments. (c) The effects of Vit.E on COX-2 and iNOS mRNA expression in LPS plus IFN- γ -induced RAW264.7 cells. (d) The effects of Vit.E on TNF- α , IL-6, and SOD2 mRNA expression in LPS plus IFN- γ -induced RAW264.7 cells. The data represent the mean ± S.D. of two independent experiments. Cells were pretreated with GIE or DEX for 3 h and then coincubated with LPS plus IFN- γ for 24 h. UN: uninduced cells; IN: untreated LPS plus IFN- γ -induced cells; DEX: cells were pretreated with DEX at 1 μ M; GIE 300: cells were pretreated with GIE 300 μ g/mL; Vit.E (150 and 300): cells were pretreated with Vit.E 150 and 300 μ g/mL, respectively. One-way ANOVA performed the comparison, and Tukey was used as a post hoc test. The degree of significance was denoted with different letters for the comparison between sample groups. p < 0.05 was considered as statistically significant.

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Gymnema inodorum (Lour.) Decne. Extract Alleviates Oxidative Stress and Inflammatory Mediators Produced by RAW264.7 Macrophages

    doi: 10.1155/2021/8658314

    Figure Lengend Snippet: (a) Cytotoxic effects of Vit.E on RAW264.7 cells for 24 h. MTT assay was used to determine cell viability. Values are expressed as a percentage of the control. (b) The effect of Vit.E compared to GIE on NO production in LPS plus IFN- γ -induced RAW264.7 cells. Nitrite concentration was determined from a sodium nitrite standard curve, and the results are expressed as a concentration ( μ M) of nitrite in a culture medium. The data represent the mean ± S.D. of three independent experiments. (c) The effects of Vit.E on COX-2 and iNOS mRNA expression in LPS plus IFN- γ -induced RAW264.7 cells. (d) The effects of Vit.E on TNF- α , IL-6, and SOD2 mRNA expression in LPS plus IFN- γ -induced RAW264.7 cells. The data represent the mean ± S.D. of two independent experiments. Cells were pretreated with GIE or DEX for 3 h and then coincubated with LPS plus IFN- γ for 24 h. UN: uninduced cells; IN: untreated LPS plus IFN- γ -induced cells; DEX: cells were pretreated with DEX at 1 μ M; GIE 300: cells were pretreated with GIE 300 μ g/mL; Vit.E (150 and 300): cells were pretreated with Vit.E 150 and 300 μ g/mL, respectively. One-way ANOVA performed the comparison, and Tukey was used as a post hoc test. The degree of significance was denoted with different letters for the comparison between sample groups. p < 0.05 was considered as statistically significant.

    Article Snippet: The murine macrophage cell line RAW264.7 (CLS Cell Lines Service GmbH., Eppelheim, Germany) was cultured in RPMI-1640 medium (Cat. No. 1IVG1-31800022) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Cat. No. 1IVG7-10270-106) and 100 U/mL penicillin-streptomycin (Cat. No. 1IVG7-15140-122) (GIBCO, Grand Island, NY, USA).

    Techniques: MTT Assay, Concentration Assay, Expressing

    Effects of O. indicum on cell viability in RAW264.7 cells. Cells were treated with different concentrations of O. indicum for 24 h. Cell viability was determined by the MTT assay. CON: cells without O. indicum ; 50–1,000: cells were treated with O. indicum at the concentration range of 50–1,000 μ g mL −1 . Values are expressed as a percentage of the control. The data represent the mean ± SD of three independent experiments. Bars marked with different letters are significantly different at p < 0.05 as determined by one-way ANOVA with Tukey's post hoc test.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Intracellular ROS Scavenging and Anti-Inflammatory Activities of Oroxylum indicum Kurz (L.) Extract in LPS plus IFN- γ -Activated RAW264.7 Macrophages

    doi: 10.1155/2020/7436920

    Figure Lengend Snippet: Effects of O. indicum on cell viability in RAW264.7 cells. Cells were treated with different concentrations of O. indicum for 24 h. Cell viability was determined by the MTT assay. CON: cells without O. indicum ; 50–1,000: cells were treated with O. indicum at the concentration range of 50–1,000 μ g mL −1 . Values are expressed as a percentage of the control. The data represent the mean ± SD of three independent experiments. Bars marked with different letters are significantly different at p < 0.05 as determined by one-way ANOVA with Tukey's post hoc test.

    Article Snippet: The RAW264.7 macrophage cells (Cell Lines Service, Eppelheim, Germany) were cultured at 37°C, 5% CO 2 in RPMI-1640 medium supplemented with 10% heat-inactivated FBS and 100 U mL −1 penicillin-streptomycin.

    Techniques: MTT Assay, Concentration Assay

    Effects of O. indicum on the intracellular ROS production in LPS plus IFN- γ -activated RAW264.7 cells. Cells were pretreated with different concentrations of O. indicum for 3 h and then activated with LPS plus IFN- γ for 24 h. Cell viability and ROS intensity are expressed as a percentage of the control. UNA: unactivated cells; CON: cells without O. indicum ; NAC: cells were pretreated with NAC at 3 mM; VIT.C: cells were pretreated with VIT.C at 50 μ g mL −1 ; and 50, 100, and 200: cells were pretreated with O. indicum at 50, 100, and 200 μ g mL −1 , respectively. Values are expressed as a percentage of the control. The data represent the mean ± SD of three independent experiments. Bars marked with different letters are significantly different at p < 0.05 as determined by one-way ANOVA with Tukey's post hoc test.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Intracellular ROS Scavenging and Anti-Inflammatory Activities of Oroxylum indicum Kurz (L.) Extract in LPS plus IFN- γ -Activated RAW264.7 Macrophages

    doi: 10.1155/2020/7436920

    Figure Lengend Snippet: Effects of O. indicum on the intracellular ROS production in LPS plus IFN- γ -activated RAW264.7 cells. Cells were pretreated with different concentrations of O. indicum for 3 h and then activated with LPS plus IFN- γ for 24 h. Cell viability and ROS intensity are expressed as a percentage of the control. UNA: unactivated cells; CON: cells without O. indicum ; NAC: cells were pretreated with NAC at 3 mM; VIT.C: cells were pretreated with VIT.C at 50 μ g mL −1 ; and 50, 100, and 200: cells were pretreated with O. indicum at 50, 100, and 200 μ g mL −1 , respectively. Values are expressed as a percentage of the control. The data represent the mean ± SD of three independent experiments. Bars marked with different letters are significantly different at p < 0.05 as determined by one-way ANOVA with Tukey's post hoc test.

    Article Snippet: The RAW264.7 macrophage cells (Cell Lines Service, Eppelheim, Germany) were cultured at 37°C, 5% CO 2 in RPMI-1640 medium supplemented with 10% heat-inactivated FBS and 100 U mL −1 penicillin-streptomycin.

    Techniques:

    Effects of O. indicum on (a) NO production, (b) proinflammatory cytokines IL-6, and (c) TNF- α secretion in LPS plus IFN- γ -activated RAW264.7 cells. Cells were pretreated with different concentrations of O. indicum for 3 h and then activated with LPS plus IFN- γ for 24 h UNA: unactivated cells; CON: cells without O. indicum ; DEX: cells were pretreated with DEX at 1 μ M; 50, 100, and 200: cells were pretreated with O. indicum at 50, 100, and 200 μ g mL −1 , respectively. The data represent the mean ± SD of three independent experiments. Bars marked with different letters are significantly different at p < 0.05 as determined by one-way ANOVA with Tukey's post hoc test.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Intracellular ROS Scavenging and Anti-Inflammatory Activities of Oroxylum indicum Kurz (L.) Extract in LPS plus IFN- γ -Activated RAW264.7 Macrophages

    doi: 10.1155/2020/7436920

    Figure Lengend Snippet: Effects of O. indicum on (a) NO production, (b) proinflammatory cytokines IL-6, and (c) TNF- α secretion in LPS plus IFN- γ -activated RAW264.7 cells. Cells were pretreated with different concentrations of O. indicum for 3 h and then activated with LPS plus IFN- γ for 24 h UNA: unactivated cells; CON: cells without O. indicum ; DEX: cells were pretreated with DEX at 1 μ M; 50, 100, and 200: cells were pretreated with O. indicum at 50, 100, and 200 μ g mL −1 , respectively. The data represent the mean ± SD of three independent experiments. Bars marked with different letters are significantly different at p < 0.05 as determined by one-way ANOVA with Tukey's post hoc test.

    Article Snippet: The RAW264.7 macrophage cells (Cell Lines Service, Eppelheim, Germany) were cultured at 37°C, 5% CO 2 in RPMI-1640 medium supplemented with 10% heat-inactivated FBS and 100 U mL −1 penicillin-streptomycin.

    Techniques:

    Effects of O. indicum on the morphology of LPS plus IFN- γ -activated RAW264.7 cells. Haematoxylin staining of 6 different groups of the sample. a: unactivated RAW264.7 cells; b: activated RAW264.7 cells (cells without O. indicum ); c: cells were pretreated with DEX at 1 μ M; d, e, and f: cells were pretreated with O. indicum at 50, 100, and 200 μ g mL −1 , respectively (original magnification at ×400, scale bar; 20 μ m).

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Intracellular ROS Scavenging and Anti-Inflammatory Activities of Oroxylum indicum Kurz (L.) Extract in LPS plus IFN- γ -Activated RAW264.7 Macrophages

    doi: 10.1155/2020/7436920

    Figure Lengend Snippet: Effects of O. indicum on the morphology of LPS plus IFN- γ -activated RAW264.7 cells. Haematoxylin staining of 6 different groups of the sample. a: unactivated RAW264.7 cells; b: activated RAW264.7 cells (cells without O. indicum ); c: cells were pretreated with DEX at 1 μ M; d, e, and f: cells were pretreated with O. indicum at 50, 100, and 200 μ g mL −1 , respectively (original magnification at ×400, scale bar; 20 μ m).

    Article Snippet: The RAW264.7 macrophage cells (Cell Lines Service, Eppelheim, Germany) were cultured at 37°C, 5% CO 2 in RPMI-1640 medium supplemented with 10% heat-inactivated FBS and 100 U mL −1 penicillin-streptomycin.

    Techniques: Staining

    Effects of O. indicum on biomolecular changes detected by FTIR. (a) Average original FTIR spectra (3500–950 cm −1 ). (b) Average the secondary derivative spectra of lipid regions (3000–2800 cm −1 ) and protein regions (1800–1400 cm −1 ). (c) The bar graph of integrated areas of lipid regions (3000–2800 cm −1 ) and protein regions (1800–1400 cm −1 ). The data obtained from unactivated RAW264.7 cells ( n = 120), activated RAW264.7 (LPS plus IFN- γ ) ( n = 120), and activated RAW264.7 (LPS plus IFN- γ ) exposed to 1 μ M DEX ( n = 157) or 200 μ g mL −1 of O. indicum ( n = 135). Data are represented as means ± SD for three replicates. Bars marked with different letters are significantly different at p < 0.05 as determined by one-way ANOVA with Tukey's post hoc test.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Intracellular ROS Scavenging and Anti-Inflammatory Activities of Oroxylum indicum Kurz (L.) Extract in LPS plus IFN- γ -Activated RAW264.7 Macrophages

    doi: 10.1155/2020/7436920

    Figure Lengend Snippet: Effects of O. indicum on biomolecular changes detected by FTIR. (a) Average original FTIR spectra (3500–950 cm −1 ). (b) Average the secondary derivative spectra of lipid regions (3000–2800 cm −1 ) and protein regions (1800–1400 cm −1 ). (c) The bar graph of integrated areas of lipid regions (3000–2800 cm −1 ) and protein regions (1800–1400 cm −1 ). The data obtained from unactivated RAW264.7 cells ( n = 120), activated RAW264.7 (LPS plus IFN- γ ) ( n = 120), and activated RAW264.7 (LPS plus IFN- γ ) exposed to 1 μ M DEX ( n = 157) or 200 μ g mL −1 of O. indicum ( n = 135). Data are represented as means ± SD for three replicates. Bars marked with different letters are significantly different at p < 0.05 as determined by one-way ANOVA with Tukey's post hoc test.

    Article Snippet: The RAW264.7 macrophage cells (Cell Lines Service, Eppelheim, Germany) were cultured at 37°C, 5% CO 2 in RPMI-1640 medium supplemented with 10% heat-inactivated FBS and 100 U mL −1 penicillin-streptomycin.

    Techniques:

    PCA analysis of FTIR spectral range 3000–2800 cm −1 and 1800–1400 cm −1 giving (a) PCA 3D score plot and (b) PCA loading plot. PCA score plots showed distinct clustering between unactivated RAW264.7 cells ( n = 120), activated RAW264.7 (LPS plus IFN- γ ) ( n = 120), and activated RAW264.7 (LPS plus IFN- γ ) exposed to 1 μ M DEX ( n = 157) or 200 μ g mL −1 of O. indicum ( n = 135). PCA loading plots identify biomarker differences over a spectral range of samples.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Intracellular ROS Scavenging and Anti-Inflammatory Activities of Oroxylum indicum Kurz (L.) Extract in LPS plus IFN- γ -Activated RAW264.7 Macrophages

    doi: 10.1155/2020/7436920

    Figure Lengend Snippet: PCA analysis of FTIR spectral range 3000–2800 cm −1 and 1800–1400 cm −1 giving (a) PCA 3D score plot and (b) PCA loading plot. PCA score plots showed distinct clustering between unactivated RAW264.7 cells ( n = 120), activated RAW264.7 (LPS plus IFN- γ ) ( n = 120), and activated RAW264.7 (LPS plus IFN- γ ) exposed to 1 μ M DEX ( n = 157) or 200 μ g mL −1 of O. indicum ( n = 135). PCA loading plots identify biomarker differences over a spectral range of samples.

    Article Snippet: The RAW264.7 macrophage cells (Cell Lines Service, Eppelheim, Germany) were cultured at 37°C, 5% CO 2 in RPMI-1640 medium supplemented with 10% heat-inactivated FBS and 100 U mL −1 penicillin-streptomycin.

    Techniques: Biomarker Assay