Journal: PLOS One

Article Title: Conditioned medium of engineering macrophages combined with soluble microneedles promote diabetic wound healing

doi: 10.1371/journal.pone.0316398

Figure Lengend Snippet: A) The green fluorescence in macrophages after transfection. B) ELISA results of the content of FGF2 in macrophages conditioned medium after transfection. C) The mRNA expression of FGF2 gene was measured via RT-qPCR after transfection. D) RFP was used to label FGF2 in RAW 264.7 cells, and the red fluorescence was observed in FGF2 OE group. Data are presented as mean ± SD. * p < 0.05, **p < 0.01, ****p < 0.0001; OE overexpression; scale bar = 20 μm.

Article Snippet: The prepared FGF2 overexpressed RAW 264.7 cells were embedded in paraffin wax and treated with the primary antibody (fgf2-rfp, Servicebio, 1:1000).

Techniques: Fluorescence, Transfection, Enzyme-linked Immunosorbent Assay, Expressing, Quantitative RT-PCR, Over Expression

Journal: PLOS One

Article Title: Conditioned medium of engineering macrophages combined with soluble microneedles promote diabetic wound healing

doi: 10.1371/journal.pone.0316398

Figure Lengend Snippet: A) Venn diagram of protein quantity analysis results of FGF2 OE group and control group. B) Venn diagram of protein quantity analysis results of FGF2 OE group. C) Venn diagram of protein quantity analysis results of control group. D) Pearson correlation coefficients of all quantitative protein expression between each two samples. E) Cluster heat map of the variation of the study sample.

Article Snippet: The prepared FGF2 overexpressed RAW 264.7 cells were embedded in paraffin wax and treated with the primary antibody (fgf2-rfp, Servicebio, 1:1000).

Techniques: Control, Expressing

Journal: PLOS One

Article Title: Conditioned medium of engineering macrophages combined with soluble microneedles promote diabetic wound healing

doi: 10.1371/journal.pone.0316398

Figure Lengend Snippet: A) Representative images of the cell scratch assay of EA.hy 926 cells treated with PBS (control group), macrophages conditioned medium (RAW 264.7 CM group) and conditioned medium of FGF2 overexpressed macrophages (FGF2 OE group) for 24 hours. B) Quantitative analysis of scratch closure evaluated via cell scratch assay. C) The body weight change in each group. D) Photographs of mouse wounds at different time points following various treatments. E) Statistical analysis of the wound areas. F) Simulated in vivo wound closure traces for different treatments. CM: Conditioned medium, MN: Microneedles, OE: Overexpression, * p < 0.05, **p < 0.01, ****p < 0.0001.

Article Snippet: The prepared FGF2 overexpressed RAW 264.7 cells were embedded in paraffin wax and treated with the primary antibody (fgf2-rfp, Servicebio, 1:1000).

Techniques: Wound Healing Assay, Control, In Vivo, Over Expression