macrophage raw 264 7 cell line  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH macrophage raw 264 7 cell line
    Macrophage Raw 264 7 Cell Line, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    macrophage raw 264 7 cell line  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH macrophage raw 264 7 cell line
    Macrophage Raw 264 7 Cell Line, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    macrophage raw 264 7 cell line  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH macrophage raw 264 7 cell line
    Macrophage Raw 264 7 Cell Line, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    murine macrophage cell line raw 264 7  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH murine macrophage cell line raw 264 7
    Murine Macrophage Cell Line Raw 264 7, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    murine macrophage cell line raw 264 7  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH murine macrophage cell line raw 264 7
    Cell viability of RAW 264.7 cells after incubation with different concentrations (10 −3 to 10 −8 M) of ( A ) methazolamide, ( B ) fuorosemide and ( C ) dorzolamide for 24 h (white bars, control: black bar). Viability was estimated using CellTiter-Blue ® Cell Viability Assay (n = 3; **** p < 0.0001; one-way ANOVA).
    Murine Macrophage Cell Line Raw 264 7, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Methazolamide Reduces the AQP5 mRNA Expression and Immune Cell Migration—A New Potential Drug in Sepsis Therapy?"

    Article Title: Methazolamide Reduces the AQP5 mRNA Expression and Immune Cell Migration—A New Potential Drug in Sepsis Therapy?

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms25010610

    Cell viability of RAW 264.7 cells after incubation with different concentrations (10 −3 to 10 −8 M) of ( A ) methazolamide, ( B ) fuorosemide and ( C ) dorzolamide for 24 h (white bars, control: black bar). Viability was estimated using CellTiter-Blue ® Cell Viability Assay (n = 3; **** p < 0.0001; one-way ANOVA).
    Figure Legend Snippet: Cell viability of RAW 264.7 cells after incubation with different concentrations (10 −3 to 10 −8 M) of ( A ) methazolamide, ( B ) fuorosemide and ( C ) dorzolamide for 24 h (white bars, control: black bar). Viability was estimated using CellTiter-Blue ® Cell Viability Assay (n = 3; **** p < 0.0001; one-way ANOVA).

    Techniques Used: Incubation, Viability Assay

    TNF-α cytokine production in RAW 264.7, stimulated with LPS (1 µg/mL) and simultaneous incubation with dorzolamide (dorzo) (10 −4 M), methazholamide (metha) (10 −4 M) or fuorosemide (furo) (10 −4 M) for up to 4 h (n = 3; ** p < 0.01; unpaired t -test) (white bars: stimulated cells, control: black bar).
    Figure Legend Snippet: TNF-α cytokine production in RAW 264.7, stimulated with LPS (1 µg/mL) and simultaneous incubation with dorzolamide (dorzo) (10 −4 M), methazholamide (metha) (10 −4 M) or fuorosemide (furo) (10 −4 M) for up to 4 h (n = 3; ** p < 0.01; unpaired t -test) (white bars: stimulated cells, control: black bar).

    Techniques Used: Incubation

    murine macrophage cell line raw 264 7  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH murine macrophage cell line raw 264 7
    Murine Macrophage Cell Line Raw 264 7, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    murine macrophage cell line raw 264 7  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH murine macrophage cell line raw 264 7
    Maintenance of an immunomodulating effect on RAW 264.7 cells of nebulised PLG-OMP: assessment of pro-inflammatory cytokine secretion induced by 24 h stimulation with LPS (red), stimulated with LPS for 24 h and treated with either nebulised PLG-OMP (blue) or PLG-OMP (yellow). PLG NEB: aerosol cloud collected in a bio-impinger. PLG: untreated eye drops. ( a ) Expression of IL-1β, *** p < 0.001 vs. PLG NEB and PLG; ( b ) expression of IL-6, * p < 0.05 vs. PLG NEB, ** 0.001 < p < 0.01 vs. PLG; ( c ) expression of TNF-α, ** 0.001 < p < 0.01 vs. PLG NEB and PLG. Data were normalised in relation to the total protein concentration and expressed as pg of cytokine per μg of protein.
    Murine Macrophage Cell Line Raw 264 7, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Biopharmaceutical Assessment of Mesh Aerosolised Plasminogen, a Step towards ARDS Treatment"

    Article Title: Biopharmaceutical Assessment of Mesh Aerosolised Plasminogen, a Step towards ARDS Treatment

    Journal: Pharmaceutics

    doi: 10.3390/pharmaceutics15061618

    Maintenance of an immunomodulating effect on RAW 264.7 cells of nebulised PLG-OMP: assessment of pro-inflammatory cytokine secretion induced by 24 h stimulation with LPS (red), stimulated with LPS for 24 h and treated with either nebulised PLG-OMP (blue) or PLG-OMP (yellow). PLG NEB: aerosol cloud collected in a bio-impinger. PLG: untreated eye drops. ( a ) Expression of IL-1β, *** p < 0.001 vs. PLG NEB and PLG; ( b ) expression of IL-6, * p < 0.05 vs. PLG NEB, ** 0.001 < p < 0.01 vs. PLG; ( c ) expression of TNF-α, ** 0.001 < p < 0.01 vs. PLG NEB and PLG. Data were normalised in relation to the total protein concentration and expressed as pg of cytokine per μg of protein.
    Figure Legend Snippet: Maintenance of an immunomodulating effect on RAW 264.7 cells of nebulised PLG-OMP: assessment of pro-inflammatory cytokine secretion induced by 24 h stimulation with LPS (red), stimulated with LPS for 24 h and treated with either nebulised PLG-OMP (blue) or PLG-OMP (yellow). PLG NEB: aerosol cloud collected in a bio-impinger. PLG: untreated eye drops. ( a ) Expression of IL-1β, *** p < 0.001 vs. PLG NEB and PLG; ( b ) expression of IL-6, * p < 0.05 vs. PLG NEB, ** 0.001 < p < 0.01 vs. PLG; ( c ) expression of TNF-α, ** 0.001 < p < 0.01 vs. PLG NEB and PLG. Data were normalised in relation to the total protein concentration and expressed as pg of cytokine per μg of protein.

    Techniques Used: Expressing, Protein Concentration

    murine macrophage cell line raw 264 7  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH murine macrophage cell line raw 264 7
    Murine Macrophage Cell Line Raw 264 7, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    raw 264 7 cells  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH raw 264 7 cells
    Anti-inflammatory activity of labdanum absolute and fractions. Inhibition of nitric oxide (NO) release by LPS-stimulated RAW 264.7 cells for cells exposed to labdanum absolute (left bars, blue color) and diterpene (Dit, middle bars, white color) and flavonoid (Flv, right bars; grey color) fractions, expressed as percentage of control (black bars). Results are expressed as mean ± SD ( n = 4 independent assays). Different coefficient letters mean statistical differences between values by the post-hoc Dunnett’s T3 test or Welch’s t -test ( p < 0.05).
    Raw 264 7 Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Labdanum Resin from Cistus ladanifer L.: A Natural and Sustainable Ingredient for Skin Care Cosmetics with Relevant Cosmeceutical Bioactivities"

    Article Title: Labdanum Resin from Cistus ladanifer L.: A Natural and Sustainable Ingredient for Skin Care Cosmetics with Relevant Cosmeceutical Bioactivities

    Journal: Plants

    doi: 10.3390/plants11111477

    Anti-inflammatory activity of labdanum absolute and fractions. Inhibition of nitric oxide (NO) release by LPS-stimulated RAW 264.7 cells for cells exposed to labdanum absolute (left bars, blue color) and diterpene (Dit, middle bars, white color) and flavonoid (Flv, right bars; grey color) fractions, expressed as percentage of control (black bars). Results are expressed as mean ± SD ( n = 4 independent assays). Different coefficient letters mean statistical differences between values by the post-hoc Dunnett’s T3 test or Welch’s t -test ( p < 0.05).
    Figure Legend Snippet: Anti-inflammatory activity of labdanum absolute and fractions. Inhibition of nitric oxide (NO) release by LPS-stimulated RAW 264.7 cells for cells exposed to labdanum absolute (left bars, blue color) and diterpene (Dit, middle bars, white color) and flavonoid (Flv, right bars; grey color) fractions, expressed as percentage of control (black bars). Results are expressed as mean ± SD ( n = 4 independent assays). Different coefficient letters mean statistical differences between values by the post-hoc Dunnett’s T3 test or Welch’s t -test ( p < 0.05).

    Techniques Used: Activity Assay, Inhibition

    raw 264 7 cells  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH raw 264 7 cells
    Effects of Aspergillus oryzae-fermented wheat peptone (AFWP) on amino acid composition and antioxidant activity. Effect of AFWP on amino acid composition ( A ), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity ( B ) and NO production ( C ). ( B ) AFWP or unfermented wheat peptone (UWP) was reacted with DPPH in the dark at 37 °C for 30 min. Absorbance at 517 nm was measured by spectrophotometry. Ascorbic acid (AA) was used as a control compound. ( C ) RAW 264.7 cells were incubated with AFWP or UWP in the presence of lipopolysaccharide (LPS, 1 μg/mL) for 48 h. After the incubation, levels of NO were measured, and 2-amino-4-methylpyridine (2-AM) was used as a control compound. The results were confirmed using four independent experiments. Each experiment was conducted in duplicate. All data are presented as the mean ± SEM of four independent experiments. Statistical significance of differences among the groups was assessed by one-way analysis of variance (ANOVA), followed by Tukey’s multiple-comparison test, using the GraphPad Prism 5 software. * p < 0.05 vs. control group. AFWP: Aspergillus oryzae-fermented wheat peptone, UWP: unfermented wheat peptone.
    Raw 264 7 Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Aspergillus oryzae -Fermented Wheat Peptone Enhances the Potential of Proliferation and Hydration of Human Keratinocytes through Activation of p44/42 MAPK"

    Article Title: Aspergillus oryzae -Fermented Wheat Peptone Enhances the Potential of Proliferation and Hydration of Human Keratinocytes through Activation of p44/42 MAPK

    Journal: Molecules

    doi: 10.3390/molecules26196074

    Effects of Aspergillus oryzae-fermented wheat peptone (AFWP) on amino acid composition and antioxidant activity. Effect of AFWP on amino acid composition ( A ), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity ( B ) and NO production ( C ). ( B ) AFWP or unfermented wheat peptone (UWP) was reacted with DPPH in the dark at 37 °C for 30 min. Absorbance at 517 nm was measured by spectrophotometry. Ascorbic acid (AA) was used as a control compound. ( C ) RAW 264.7 cells were incubated with AFWP or UWP in the presence of lipopolysaccharide (LPS, 1 μg/mL) for 48 h. After the incubation, levels of NO were measured, and 2-amino-4-methylpyridine (2-AM) was used as a control compound. The results were confirmed using four independent experiments. Each experiment was conducted in duplicate. All data are presented as the mean ± SEM of four independent experiments. Statistical significance of differences among the groups was assessed by one-way analysis of variance (ANOVA), followed by Tukey’s multiple-comparison test, using the GraphPad Prism 5 software. * p < 0.05 vs. control group. AFWP: Aspergillus oryzae-fermented wheat peptone, UWP: unfermented wheat peptone.
    Figure Legend Snippet: Effects of Aspergillus oryzae-fermented wheat peptone (AFWP) on amino acid composition and antioxidant activity. Effect of AFWP on amino acid composition ( A ), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity ( B ) and NO production ( C ). ( B ) AFWP or unfermented wheat peptone (UWP) was reacted with DPPH in the dark at 37 °C for 30 min. Absorbance at 517 nm was measured by spectrophotometry. Ascorbic acid (AA) was used as a control compound. ( C ) RAW 264.7 cells were incubated with AFWP or UWP in the presence of lipopolysaccharide (LPS, 1 μg/mL) for 48 h. After the incubation, levels of NO were measured, and 2-amino-4-methylpyridine (2-AM) was used as a control compound. The results were confirmed using four independent experiments. Each experiment was conducted in duplicate. All data are presented as the mean ± SEM of four independent experiments. Statistical significance of differences among the groups was assessed by one-way analysis of variance (ANOVA), followed by Tukey’s multiple-comparison test, using the GraphPad Prism 5 software. * p < 0.05 vs. control group. AFWP: Aspergillus oryzae-fermented wheat peptone, UWP: unfermented wheat peptone.

    Techniques Used: Antioxidant Activity Assay, Activity Assay, Spectrophotometry, Incubation, Software

    AFW4 exerts antioxidant activity in HaCaT cells. ( A and B ) HaCaT cells were incubated with AFW4 in the presence of TPHP (55 μM) for 24 h and then subjected to fluorescence image analysis ( A ). In addition, densitometric analysis ( B ) for fluorescence images was conducted. All data are presented as the mean ± SEM of four independent experiments. ( C ) RAW 264.7 cells were incubated with AFW4 in the presence of lipopolysaccharide (LPS, 1 μg/mL) for 48 h. After incubation, the levels of NO were measured, and 2-amino-4-methylpyridine (2-AM) was used as a control compound. The results were confirmed using four independent experiments. Each experiment was conducted in duplicate. All data are presented as the mean ± SEM of four independent experiments. Statistical significance of differences among the groups was assessed by ANOVA, followed by Tukey’s multiple-comparison test, using the GraphPad Prism 5 software. * p < 0.05 vs. LPS-treated group.
    Figure Legend Snippet: AFW4 exerts antioxidant activity in HaCaT cells. ( A and B ) HaCaT cells were incubated with AFW4 in the presence of TPHP (55 μM) for 24 h and then subjected to fluorescence image analysis ( A ). In addition, densitometric analysis ( B ) for fluorescence images was conducted. All data are presented as the mean ± SEM of four independent experiments. ( C ) RAW 264.7 cells were incubated with AFW4 in the presence of lipopolysaccharide (LPS, 1 μg/mL) for 48 h. After incubation, the levels of NO were measured, and 2-amino-4-methylpyridine (2-AM) was used as a control compound. The results were confirmed using four independent experiments. Each experiment was conducted in duplicate. All data are presented as the mean ± SEM of four independent experiments. Statistical significance of differences among the groups was assessed by ANOVA, followed by Tukey’s multiple-comparison test, using the GraphPad Prism 5 software. * p < 0.05 vs. LPS-treated group.

    Techniques Used: Antioxidant Activity Assay, Incubation, Fluorescence, Software

    sirna transfection raw264 7 macrophages  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH sirna transfection raw264 7 macrophages
    Sirna Transfection Raw264 7 Macrophages, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    CLS Cell Lines Service GmbH macrophage raw 264 7 cell line
    Macrophage Raw 264 7 Cell Line, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    CLS Cell Lines Service GmbH murine macrophage cell line raw 264 7
    Murine Macrophage Cell Line Raw 264 7, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    CLS Cell Lines Service GmbH raw 264 7 cells
    Anti-inflammatory activity of labdanum absolute and fractions. Inhibition of nitric oxide (NO) release by LPS-stimulated RAW 264.7 cells for cells exposed to labdanum absolute (left bars, blue color) and diterpene (Dit, middle bars, white color) and flavonoid (Flv, right bars; grey color) fractions, expressed as percentage of control (black bars). Results are expressed as mean ± SD ( n = 4 independent assays). Different coefficient letters mean statistical differences between values by the post-hoc Dunnett’s T3 test or Welch’s t -test ( p < 0.05).
    Raw 264 7 Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/raw 264 7 cells/product/CLS Cell Lines Service GmbH
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    CLS Cell Lines Service GmbH sirna transfection raw264 7 macrophages
    Anti-inflammatory activity of labdanum absolute and fractions. Inhibition of nitric oxide (NO) release by LPS-stimulated RAW 264.7 cells for cells exposed to labdanum absolute (left bars, blue color) and diterpene (Dit, middle bars, white color) and flavonoid (Flv, right bars; grey color) fractions, expressed as percentage of control (black bars). Results are expressed as mean ± SD ( n = 4 independent assays). Different coefficient letters mean statistical differences between values by the post-hoc Dunnett’s T3 test or Welch’s t -test ( p < 0.05).
    Sirna Transfection Raw264 7 Macrophages, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sirna transfection raw264 7 macrophages/product/CLS Cell Lines Service GmbH
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    Anti-inflammatory activity of labdanum absolute and fractions. Inhibition of nitric oxide (NO) release by LPS-stimulated RAW 264.7 cells for cells exposed to labdanum absolute (left bars, blue color) and diterpene (Dit, middle bars, white color) and flavonoid (Flv, right bars; grey color) fractions, expressed as percentage of control (black bars). Results are expressed as mean ± SD ( n = 4 independent assays). Different coefficient letters mean statistical differences between values by the post-hoc Dunnett’s T3 test or Welch’s t -test ( p < 0.05).

    Journal: Plants

    Article Title: Labdanum Resin from Cistus ladanifer L.: A Natural and Sustainable Ingredient for Skin Care Cosmetics with Relevant Cosmeceutical Bioactivities

    doi: 10.3390/plants11111477

    Figure Lengend Snippet: Anti-inflammatory activity of labdanum absolute and fractions. Inhibition of nitric oxide (NO) release by LPS-stimulated RAW 264.7 cells for cells exposed to labdanum absolute (left bars, blue color) and diterpene (Dit, middle bars, white color) and flavonoid (Flv, right bars; grey color) fractions, expressed as percentage of control (black bars). Results are expressed as mean ± SD ( n = 4 independent assays). Different coefficient letters mean statistical differences between values by the post-hoc Dunnett’s T3 test or Welch’s t -test ( p < 0.05).

    Article Snippet: Anti-inflammatory activity of labdanum absolute and fractions was evaluated as the inhibition of lipopolysaccharide (LPS)-induced nitric oxide production in Raw 264.7 cells (mouse macrophages, Abelson murine leukemia virus-induced tumor, acquired from Cell Lines Service CLS, Eppelheim, Germany), as previously described by Silva, et al. [ ].

    Techniques: Activity Assay, Inhibition