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Promega ratp
Ratp, supplied by Promega, used in various techniques. Bioz Stars score: 97/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ratp/product/Promega
Average 97 stars, based on 9 article reviews
Price from $9.99 to $1999.99
ratp - by Bioz Stars, 2020-09
97/100 stars

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Amplification:

Article Title: A novel hepatitis B virus genotyping system by using restriction fragment length polymorphism patterns of S gene amplicons
Article Snippet: .. A 5 µL of the resuspended DNA was added to an amplification mixture containing 5 µL of 10 × Taq polymerase buffer, 5 µL of 25 mmol/L deoxyribonucleotide triphosphates, 1 µL (2U) of Taq polymerase (Promega, Beijing, China) and 10 pmoL each of primers PrsS2 and S1R (total volume of 50 µL). .. The PCR profile was an initial 3 min denaturation at 94 °C, followed by 35 cycles of amplification including denaturation for 45 s at 94 °C, annealing for 60 s at 53 °C, and extension for 90 s at 72 °C.

Article Title: Prevalence and Characteristics of Basal Core Promoter Mutations in Iran and its Correlation with Acute and Chronic Hepatitis B Infection
Article Snippet: .. A 5 μL of the resuspended DNA was added to an amplification mixture containing 2.5 μL of 10× Taq polymerase buffer, 0.5 μL of 25 mmol/L deoxyribonucleotide triphosphates, 0.75 μL Mgcl2 , 0.5 μL of Taq polymerase (Promega, Beijing, China), and one μmoL each of primers Core1 and Core2 (total volume of 25 μL). .. Then, for the second cycle, a 1 μL of the first-round PCR products was added to an amplification mixture containing 5 μL of 10× Taq polymerase buffer, one μL of 25 mmol/L deoxyribonucleotide triphosphates, 1.5μL Mgcl2 ,1 μL of Taq polymerase (Promega, Beijing, China), and 2 μmoL each of primers Core1 and Core3 (total volume of 25 μL).

Article Title: Genetics of Mating and Sex Determination in the Parasitic Nematode Haemonchus contortus
Article Snippet: .. PCR amplification of microsatellite loci was performed in 20-μl reactions containing 45 m m Tris HCl (pH 8.8), 11 m m (NH4 )2 SO4 , 4.5 m m MgCl2 , 6.7 m m 2-mercaptethanol, 4.4 μ m EDTA, 113 μg/ml BSA, 2% Tween, 1 m m each deoxyribonucleotide triphosphates, 0.5 μ m of each oligonucleotide primer, and 0.2 unit of Taq polymerase (Promega, Madison, WI). .. Accurate sizing of microsatellite PCR products by capillary electrophoresis was performed using an ABI Prism 3100 genetic analyzer (Applied Biosystems, Foster City, CA).

Agarose Gel Electrophoresis:

Article Title: Peripheral, but Not Central, CB1 Antagonism Provides Food Intake-Independent Metabolic Benefits in Diet-Induced Obese Rats
Article Snippet: .. RNA integrity was assessed by performing a 1% agarose gel electrophoresis in 1 × Tris-borate-EDTA, and its concentration was determined by spectrophotometry. cDNA templates for RT-PCR were synthesized using 2 μg of total RNA, random hexamers (Microsynth, Balgach, Switzerland), deoxyribonucleotide triphosphates, RNase inhibitor, RNAsin (Catalys; Promega, Madisson, WI), and Superscript III (Invitrogen). .. Quantitative PCR was performed using SYBR Green I DNA master mixture (Applied Biosystems) according to the standard protocol and using ∼70 ng template cDNA.

In Vitro:

Article Title: κ-Opioid Receptor Is Colocalized in GnRH and KNDy Cells in the Female Ovine and Rat Brain
Article Snippet: .. Sense and antisense radiolabeled cRNA probes were generated by in vitro transcription in a reaction containing transcription buffer (Promega), 0.5 mM ribonucleotide triphosphates (rATP, rGTP, rCTP), 10 μM rat uridine 5-triphosphate, 1 μg DNA template, 10 μM dithiothreitol, 5 μM ribonuclease inhibitor (Rnasin; Promega), 20 U of T7 or SP6 RNA polymerases (Promega), and 50 μCi 35S-uridine 5-triphosphate (MP Biomedicals). .. After incubating in the reaction tube for 2 hours at 37ºC, deoxyribonuclease I was added to the reaction to degrade the DNA template followed by an additional incubation at 37ºC for 15 minutes.

Synthesized:

Article Title: Peripheral, but Not Central, CB1 Antagonism Provides Food Intake-Independent Metabolic Benefits in Diet-Induced Obese Rats
Article Snippet: .. RNA integrity was assessed by performing a 1% agarose gel electrophoresis in 1 × Tris-borate-EDTA, and its concentration was determined by spectrophotometry. cDNA templates for RT-PCR were synthesized using 2 μg of total RNA, random hexamers (Microsynth, Balgach, Switzerland), deoxyribonucleotide triphosphates, RNase inhibitor, RNAsin (Catalys; Promega, Madisson, WI), and Superscript III (Invitrogen). .. Quantitative PCR was performed using SYBR Green I DNA master mixture (Applied Biosystems) according to the standard protocol and using ∼70 ng template cDNA.

Spectrophotometry:

Article Title: Peripheral, but Not Central, CB1 Antagonism Provides Food Intake-Independent Metabolic Benefits in Diet-Induced Obese Rats
Article Snippet: .. RNA integrity was assessed by performing a 1% agarose gel electrophoresis in 1 × Tris-borate-EDTA, and its concentration was determined by spectrophotometry. cDNA templates for RT-PCR were synthesized using 2 μg of total RNA, random hexamers (Microsynth, Balgach, Switzerland), deoxyribonucleotide triphosphates, RNase inhibitor, RNAsin (Catalys; Promega, Madisson, WI), and Superscript III (Invitrogen). .. Quantitative PCR was performed using SYBR Green I DNA master mixture (Applied Biosystems) according to the standard protocol and using ∼70 ng template cDNA.

Concentration Assay:

Article Title: Peripheral, but Not Central, CB1 Antagonism Provides Food Intake-Independent Metabolic Benefits in Diet-Induced Obese Rats
Article Snippet: .. RNA integrity was assessed by performing a 1% agarose gel electrophoresis in 1 × Tris-borate-EDTA, and its concentration was determined by spectrophotometry. cDNA templates for RT-PCR were synthesized using 2 μg of total RNA, random hexamers (Microsynth, Balgach, Switzerland), deoxyribonucleotide triphosphates, RNase inhibitor, RNAsin (Catalys; Promega, Madisson, WI), and Superscript III (Invitrogen). .. Quantitative PCR was performed using SYBR Green I DNA master mixture (Applied Biosystems) according to the standard protocol and using ∼70 ng template cDNA.

Generated:

Article Title: κ-Opioid Receptor Is Colocalized in GnRH and KNDy Cells in the Female Ovine and Rat Brain
Article Snippet: .. Sense and antisense radiolabeled cRNA probes were generated by in vitro transcription in a reaction containing transcription buffer (Promega), 0.5 mM ribonucleotide triphosphates (rATP, rGTP, rCTP), 10 μM rat uridine 5-triphosphate, 1 μg DNA template, 10 μM dithiothreitol, 5 μM ribonuclease inhibitor (Rnasin; Promega), 20 U of T7 or SP6 RNA polymerases (Promega), and 50 μCi 35S-uridine 5-triphosphate (MP Biomedicals). .. After incubating in the reaction tube for 2 hours at 37ºC, deoxyribonuclease I was added to the reaction to degrade the DNA template followed by an additional incubation at 37ºC for 15 minutes.

Polymerase Chain Reaction:

Article Title: Genetics of Mating and Sex Determination in the Parasitic Nematode Haemonchus contortus
Article Snippet: .. PCR amplification of microsatellite loci was performed in 20-μl reactions containing 45 m m Tris HCl (pH 8.8), 11 m m (NH4 )2 SO4 , 4.5 m m MgCl2 , 6.7 m m 2-mercaptethanol, 4.4 μ m EDTA, 113 μg/ml BSA, 2% Tween, 1 m m each deoxyribonucleotide triphosphates, 0.5 μ m of each oligonucleotide primer, and 0.2 unit of Taq polymerase (Promega, Madison, WI). .. Accurate sizing of microsatellite PCR products by capillary electrophoresis was performed using an ABI Prism 3100 genetic analyzer (Applied Biosystems, Foster City, CA).

Article Title: Optimized enzymatic dual functions of PaPrx protein by proton irradiation
Article Snippet: .. Briefly, PCR reactions were conducted in 20 µl mixtures containing 10 ng of genomic DNA, 0.2 µM deoxyribonucleotide triphosphates, 20 pmol of each primer set for PaPrx (forward primer: 5′-ccgctcgagatgagcgtactcgta, reverse primer: 3′-cccaagcttttacagcttgctggc), and 1 unit of Taq DNA polymerase (Promega) in a standard PCR buffer. .. The following conditions were used for PCR: denaturation for 1 cycle at 94°C for 60 s; 35 cycles each at 94°C for 30 s, at 50°C for 45 s, and at 72°C for 45 s; and finally 1 cycle at 72°C for 10 min.

Reverse Transcription Polymerase Chain Reaction:

Article Title: Peripheral, but Not Central, CB1 Antagonism Provides Food Intake-Independent Metabolic Benefits in Diet-Induced Obese Rats
Article Snippet: .. RNA integrity was assessed by performing a 1% agarose gel electrophoresis in 1 × Tris-borate-EDTA, and its concentration was determined by spectrophotometry. cDNA templates for RT-PCR were synthesized using 2 μg of total RNA, random hexamers (Microsynth, Balgach, Switzerland), deoxyribonucleotide triphosphates, RNase inhibitor, RNAsin (Catalys; Promega, Madisson, WI), and Superscript III (Invitrogen). .. Quantitative PCR was performed using SYBR Green I DNA master mixture (Applied Biosystems) according to the standard protocol and using ∼70 ng template cDNA.

Injection:

Article Title: Biophysical Mode-of-Action and Selectivity Analysis of Allosteric Inhibitors of Hepatitis C Virus (HCV) Polymerase
Article Snippet: .. The NS5B Δ21 polymerase and/or NS5B Δ21 supplemented with 750 µM ribonucleotide triphosphates (rNTPs) (Promega) were injected over the surface containing DNA/RNA hybrid and over the reference surface with immobilized streptavidin ( ). .. The effect of inhibitors on polymerase activity was tested by adding 600 nM of filibuvir or lomibuvir to the NS5B/rNTPs mixture.

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    Promega ratp
    Ratp, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ratp/product/Promega
    Average 91 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    ratp - by Bioz Stars, 2020-09
    91/100 stars
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