Structured Review

Promega ratp
Ratp, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ratp/product/Promega
Average 99 stars, based on 32 article reviews
Price from $9.99 to $1999.99
ratp - by Bioz Stars, 2020-01
99/100 stars

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Centrifugation:

Article Title: CR1-mediated ATP Release by Human Red Blood Cells Promotes CR1 Clustering and Modulates the Immune Transfer Process *
Article Snippet: Cells were incubated at 37 °C (20 min) with anti-CR1 monoclonal antibody (3C10, 5 μg/ml) followed by centrifugation at 300 × g (2 min) and then washed in the same medium. .. An ATP standard using rATP (Promega) was prepared each day in the following concentrations: 0.5, 1, 5, 10, 50, 100, and 200 n m .

Article Title: Epithelial-Cell-Derived Phospholipase A2 Group 1B Is an Endogenous Anthelmintic
Article Snippet: The homogenate was incubated for 10 min at room temperature before centrifugation at 1000 g for 3 min. 200 μL of the supernatant was transferred to a 96 well opaque-walled plate and incubated for 10 min at room temperature before recording luminescence. .. An ATP standard curve was generated by using recombinant ATP (Promega) as detailed in the CellTiter-Glo® Luminescent Cell Viability Assay instructions.

Amplification:

Article Title: Species-Specific Marker Discovery in Tilapia
Article Snippet: Ligation was performed over 3.5 h at 22 °C by addition of a further 3 µL of a ligation mix including 4 mM rATP (Promega, UK), and 2000 cohesive-end units of T4 ligase (NEB) in 1× CutSmart buffer. .. Following gel purification (MinElute Gel Extraction Kit, Qiagen, UK), the eluted size-selected template DNA (60 µL in EB buffer) was PCR amplified (13–14 cycles PCR dependent on library; 24 separate 12.5 µL reactions, each with 1 µL template DNA) using a high fidelity Taq polymerase (Q5 Hot Start High-Fidelity DNA Polymerase, NEB).

Article Title: Gene-centromere mapping in meiotic gynogenetic European seabass
Article Snippet: Ligation was performed over 40 min at 22 °C by addition of a further 3 μL of a ligation mix comprising 4 mM rATP (Promega, UK), and 2000 cohesive-end units of T4 ligase (NEB) in 1× CutSmart buffer. .. Following gel purification (MinElute Gel Extraction Kit, Qiagen, UK) the eluted size-selected template DNA (60 μL in EB buffer) was PCR amplified (11 cycles PCR; 28 separate 12.5 μL reactions, each with 1 μL template DNA) using a high fidelity Taq polymerase (Q5 Hot Start High-Fidelity DNA Polymerase, NEB).

Construct:

Article Title: A High Density Genetic Map Derived from RAD Sequencing and Its Application in QTL Analysis of Yield-Related Traits in Vigna unguiculata
Article Snippet: RAD libraries were constructed following a protocol from Baird et al. ( ). .. The ligation reaction was incubated at room temperature (RT) for 20 min in a final volume of 10 μL containing 2.5 μL P1Adapter (100 nM), 1 μL rATP (100 mM) (Promega), 1 μL 10 × EcoR I buffer, 0.5 μL T4 DNA Ligase (1,000 U) (NEB), and 5 μL H2 O.

Article Title: Population genomics of Culiseta melanura, the principal vector of Eastern equine encephalitis virus in the United States
Article Snippet: DNA digestion and library construction Double-digest restriction site-associated DNA libraries were constructed from two populations at a time, in batches of twelve mosquitoes, with six individuals from each population per batch. .. Adaptors containing sample barcodes were then ligated to each individual sample in a 25 μl reaction with 17 μl of digested DNA, 2.5 μl of 10X T4 DNA Ligase Reaction Buffer (NEB), 1 μl of 10 μM read 1 adaptor, 2 μl of 10 μM read 2 adaptor, 1.5 μl of 10μM rATP (Promega, Madison, WI, USA), and 100 units of T4 DNA Ligase.

Enzyme-linked Immunosorbent Assay:

Article Title: Transcriptional and Functional Analysis of Bifidobacterium animalis subsp. lactis Exposure to Tetracycline
Article Snippet: Data were generated in relative luminescence units (RLU), which were not back calculated to ATP concentrations, although a standard of 10 mM rATP (p/n P113B; Promega) was added to each run to ensure run-to-run consistency. .. The tetracycline standard dilutions were compared to the standards in the MaxSignal Tetracycline enzyme-limited immunosorbent assay (ELISA) kit (p/n 1016-04E; Bioo Scientific Corp., Austin, TX).

Incubation:

Article Title: Exposure to the Proton Scavenger Glycine under Alkaline Conditions Induces Escherichia coli Viability Loss
Article Snippet: A test sample of 100 µl i.e. both the bacterial population and the incubation medium, was taken from the exposed resuspended bacterial populations and mixed with an equal volume of BacTiter-GloTM Reagent. .. A calibration curve with adequate dilutions of pure rATP (Promega, Madison, WI, USA) was measured for each buffer prepared.

Article Title: Genetic diversity and structure in Arapaima gigas populations from Amazon and Araguaia-Tocantins river basins
Article Snippet: After cooling the reactions to room temperature, 2.5 μL of a premade barcode-adapter mix was added to the digested DNA, and incubated at room temperature for 10 min. .. Ligation was performed over 75 min at 22 °C by addition of a further 2.5 μL of a ligation mix comprising 4 mM rATP (Promega, UK), and 2000 cohesive-end units of T4 ligase (NEB) in 1× CutSmart buffer.

Article Title: Species-Specific Marker Discovery in Tilapia
Article Snippet: The reactions were then cooled to c. 22 °C, 3 µL of a premade barcode/adapter mix was added to each digested DNA sample and incubated at 22 °C for 10 min. .. Ligation was performed over 3.5 h at 22 °C by addition of a further 3 µL of a ligation mix including 4 mM rATP (Promega, UK), and 2000 cohesive-end units of T4 ligase (NEB) in 1× CutSmart buffer.

Article Title: A High Density Genetic Map Derived from RAD Sequencing and Its Application in QTL Analysis of Yield-Related Traits in Vigna unguiculata
Article Snippet: .. The ligation reaction was incubated at room temperature (RT) for 20 min in a final volume of 10 μL containing 2.5 μL P1Adapter (100 nM), 1 μL rATP (100 mM) (Promega), 1 μL 10 × EcoR I buffer, 0.5 μL T4 DNA Ligase (1,000 U) (NEB), and 5 μL H2 O. ..

Article Title: Population genomics of Culiseta melanura, the principal vector of Eastern equine encephalitis virus in the United States
Article Snippet: Adaptors containing sample barcodes were then ligated to each individual sample in a 25 μl reaction with 17 μl of digested DNA, 2.5 μl of 10X T4 DNA Ligase Reaction Buffer (NEB), 1 μl of 10 μM read 1 adaptor, 2 μl of 10 μM read 2 adaptor, 1.5 μl of 10μM rATP (Promega, Madison, WI, USA), and 100 units of T4 DNA Ligase. .. Ligation reactions were incubated at room temperature for 30 minutes, then heat shocked at 65°C for 10 minutes, then cooled 2°C every minute to 4°C.

Article Title: Gene-centromere mapping in meiotic gynogenetic European seabass
Article Snippet: After cooling the reactions to room temperature, 3 μL of a premade barcode-adapter mix was added to the digested DNA, and incubated at room temperature for 10 min. .. Ligation was performed over 40 min at 22 °C by addition of a further 3 μL of a ligation mix comprising 4 mM rATP (Promega, UK), and 2000 cohesive-end units of T4 ligase (NEB) in 1× CutSmart buffer.

Article Title: TPL-2 restricts Ccl24-dependent immunity to Heligmosomoides polygyrus
Article Snippet: 200 μL of the supernatant was transferred to a 96 well opaque-walled plate and incubated for 10 minutes at room temperature before recording luminescence. .. An ATP standard curve was generated by using recombinant ATP (Promega) as detailed in the CellTiter-Glo Luminescent Cell Viability Assay instructions.

Article Title: CR1-mediated ATP Release by Human Red Blood Cells Promotes CR1 Clustering and Modulates the Immune Transfer Process *
Article Snippet: Cell suspension (55 μl) were pipetted into a black 96-well plate and secondary antibody (goat anti-mouse, Jackson, 115-005-003) was added in the same concentration (volume: 5 μl), followed by incubation at room temperature (70 min). .. An ATP standard using rATP (Promega) was prepared each day in the following concentrations: 0.5, 1, 5, 10, 50, 100, and 200 n m .

Article Title: The extruded non-template strand determines the architecture of R-loops
Article Snippet: .. In vitro transcription of R-loops 1875 ng of circular plasmids were incubated at 37°C with 50 U of T3 RNA Polymerase (Promega) in a transcription buffer (40 mM Tris–HCl pH 7.9, 6 mM MgCl2 , 2 mM spermidine, 10 mM NaCl, 20 mM DTT, 0,05% Tween-20) containing 0,25 mM of rATP, rCTP, rUTP and rGTP (Promega). ..

Article Title: Epithelial-Cell-Derived Phospholipase A2 Group 1B Is an Endogenous Anthelmintic
Article Snippet: The homogenate was incubated for 10 min at room temperature before centrifugation at 1000 g for 3 min. 200 μL of the supernatant was transferred to a 96 well opaque-walled plate and incubated for 10 min at room temperature before recording luminescence. .. An ATP standard curve was generated by using recombinant ATP (Promega) as detailed in the CellTiter-Glo® Luminescent Cell Viability Assay instructions.

Article Title: NMDA receptor activity downregulates KCC2 resulting in depolarizing GABAA receptor mediated currents
Article Snippet: .. In vitro phosphorylation of fusion proteins We incubated 0.5 mg of His-C fusion protein with 50 μM rATP (Promega) and 5 ng of purified PKC (EMD Biosciences) in a buffer containing (in mM): 20 HEPES (pH 7.5) and 10 MgCl2 for 10 min at 30 °C and terminated by the addition of 2x SDS-PAGE sample buffer. ..

Luciferase:

Article Title: CR1-mediated ATP Release by Human Red Blood Cells Promotes CR1 Clustering and Modulates the Immune Transfer Process *
Article Snippet: ATP release was measured using the GloMax®-Multi+ Detection System (Promega) and ENLITEN® (luciferase/luciferin) reagent, which was injected in equal volumes to the sample into each well and bioluminescence was read. .. An ATP standard using rATP (Promega) was prepared each day in the following concentrations: 0.5, 1, 5, 10, 50, 100, and 200 n m .

Activity Assay:

Article Title: Genetic diversity and structure in Arapaima gigas populations from Amazon and Araguaia-Tocantins river basins
Article Snippet: The barcoded adapters were designed such that adapter–genomic DNA ligations did not reconstitute RE sites, while residual RE activity limited concatemerization of genomic fragments during ligation. .. Ligation was performed over 75 min at 22 °C by addition of a further 2.5 μL of a ligation mix comprising 4 mM rATP (Promega, UK), and 2000 cohesive-end units of T4 ligase (NEB) in 1× CutSmart buffer.

Article Title: Species-Specific Marker Discovery in Tilapia
Article Snippet: The barcoded adapters were designed such that adapter– genomic DNA ligations did not reconstitute RE sites, while residual RE activity limited concatemerisation of genomic fragments. .. Ligation was performed over 3.5 h at 22 °C by addition of a further 3 µL of a ligation mix including 4 mM rATP (Promega, UK), and 2000 cohesive-end units of T4 ligase (NEB) in 1× CutSmart buffer.

Article Title: Gene-centromere mapping in meiotic gynogenetic European seabass
Article Snippet: The barcoded adapters were designed such that adapter–genomic DNA ligations did not reconstitute RE sites, while residual RE activity limited concatemerization of genomic fragments during ligation. .. Ligation was performed over 40 min at 22 °C by addition of a further 3 μL of a ligation mix comprising 4 mM rATP (Promega, UK), and 2000 cohesive-end units of T4 ligase (NEB) in 1× CutSmart buffer.

Article Title: Targeting Glycolysis with Epigallocatechin-3-Gallate Enhances the Efficacy of Chemotherapeutics in Pancreatic Cancer Cells and Xenografts
Article Snippet: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (≥97.5%), D-(+)-Glucose (≥99.5%), Oligomycin (≥90%), 2-Deoxy-d -glucose (≥99%), Irinotecan hydrochloride, Oxaliplatin, Catalase, N -Acetyl-l -cysteine (≥99%), DL-Buthionine-sulfoximine (≥99%), RIPA lysis buffer, Halt Protease Inhibitor Cocktail, Phosphatase Inhibitor Cocktail, and the following kits: Phosphofructokinase Activity Colorimetric assay and Pyruvate Kinase Activity assay were purchased from MilliporeSigma (St. Louis, MO, USA). .. CellTiter-Glo® reagent and rATP were purchased from Promega (Madison, WI, USA).

Kinase Assay:

Article Title: Targeting Glycolysis with Epigallocatechin-3-Gallate Enhances the Efficacy of Chemotherapeutics in Pancreatic Cancer Cells and Xenografts
Article Snippet: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (≥97.5%), D-(+)-Glucose (≥99.5%), Oligomycin (≥90%), 2-Deoxy-d -glucose (≥99%), Irinotecan hydrochloride, Oxaliplatin, Catalase, N -Acetyl-l -cysteine (≥99%), DL-Buthionine-sulfoximine (≥99%), RIPA lysis buffer, Halt Protease Inhibitor Cocktail, Phosphatase Inhibitor Cocktail, and the following kits: Phosphofructokinase Activity Colorimetric assay and Pyruvate Kinase Activity assay were purchased from MilliporeSigma (St. Louis, MO, USA). .. CellTiter-Glo® reagent and rATP were purchased from Promega (Madison, WI, USA).

Gel Purification:

Article Title: Species-Specific Marker Discovery in Tilapia
Article Snippet: Ligation was performed over 3.5 h at 22 °C by addition of a further 3 µL of a ligation mix including 4 mM rATP (Promega, UK), and 2000 cohesive-end units of T4 ligase (NEB) in 1× CutSmart buffer. .. Following gel purification (MinElute Gel Extraction Kit, Qiagen, UK), the eluted size-selected template DNA (60 µL in EB buffer) was PCR amplified (13–14 cycles PCR dependent on library; 24 separate 12.5 µL reactions, each with 1 µL template DNA) using a high fidelity Taq polymerase (Q5 Hot Start High-Fidelity DNA Polymerase, NEB).

Article Title: Gene-centromere mapping in meiotic gynogenetic European seabass
Article Snippet: Ligation was performed over 40 min at 22 °C by addition of a further 3 μL of a ligation mix comprising 4 mM rATP (Promega, UK), and 2000 cohesive-end units of T4 ligase (NEB) in 1× CutSmart buffer. .. Following gel purification (MinElute Gel Extraction Kit, Qiagen, UK) the eluted size-selected template DNA (60 μL in EB buffer) was PCR amplified (11 cycles PCR; 28 separate 12.5 μL reactions, each with 1 μL template DNA) using a high fidelity Taq polymerase (Q5 Hot Start High-Fidelity DNA Polymerase, NEB).

Colorimetric Assay:

Article Title: Targeting Glycolysis with Epigallocatechin-3-Gallate Enhances the Efficacy of Chemotherapeutics in Pancreatic Cancer Cells and Xenografts
Article Snippet: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (≥97.5%), D-(+)-Glucose (≥99.5%), Oligomycin (≥90%), 2-Deoxy-d -glucose (≥99%), Irinotecan hydrochloride, Oxaliplatin, Catalase, N -Acetyl-l -cysteine (≥99%), DL-Buthionine-sulfoximine (≥99%), RIPA lysis buffer, Halt Protease Inhibitor Cocktail, Phosphatase Inhibitor Cocktail, and the following kits: Phosphofructokinase Activity Colorimetric assay and Pyruvate Kinase Activity assay were purchased from MilliporeSigma (St. Louis, MO, USA). .. CellTiter-Glo® reagent and rATP were purchased from Promega (Madison, WI, USA).

Ligation:

Article Title: Genetic diversity and structure in Arapaima gigas populations from Amazon and Araguaia-Tocantins river basins
Article Snippet: .. Ligation was performed over 75 min at 22 °C by addition of a further 2.5 μL of a ligation mix comprising 4 mM rATP (Promega, UK), and 2000 cohesive-end units of T4 ligase (NEB) in 1× CutSmart buffer. .. The reactions were terminated by addition of 20 μL PB buffer (Minelute PCR Purification Kit; Qiagen UK).

Article Title: Species-Specific Marker Discovery in Tilapia
Article Snippet: .. Ligation was performed over 3.5 h at 22 °C by addition of a further 3 µL of a ligation mix including 4 mM rATP (Promega, UK), and 2000 cohesive-end units of T4 ligase (NEB) in 1× CutSmart buffer. .. The pooled library sample was column-purified (MinElute PCR Purification Kit, Qiagen, UK), and eluted in 80 µL EB buffer (Qiagen, UK).

Article Title: A High Density Genetic Map Derived from RAD Sequencing and Its Application in QTL Analysis of Yield-Related Traits in Vigna unguiculata
Article Snippet: .. The ligation reaction was incubated at room temperature (RT) for 20 min in a final volume of 10 μL containing 2.5 μL P1Adapter (100 nM), 1 μL rATP (100 mM) (Promega), 1 μL 10 × EcoR I buffer, 0.5 μL T4 DNA Ligase (1,000 U) (NEB), and 5 μL H2 O. ..

Article Title: Population genomics of Culiseta melanura, the principal vector of Eastern equine encephalitis virus in the United States
Article Snippet: Library construction was similar to the ‘optimized protocol’ of Hoffberg et al. [ ], although pooling occurred prior to size selection, rather than after adaptor ligation. .. Adaptors containing sample barcodes were then ligated to each individual sample in a 25 μl reaction with 17 μl of digested DNA, 2.5 μl of 10X T4 DNA Ligase Reaction Buffer (NEB), 1 μl of 10 μM read 1 adaptor, 2 μl of 10 μM read 2 adaptor, 1.5 μl of 10μM rATP (Promega, Madison, WI, USA), and 100 units of T4 DNA Ligase.

Article Title: Gene-centromere mapping in meiotic gynogenetic European seabass
Article Snippet: .. Ligation was performed over 40 min at 22 °C by addition of a further 3 μL of a ligation mix comprising 4 mM rATP (Promega, UK), and 2000 cohesive-end units of T4 ligase (NEB) in 1× CutSmart buffer. .. The pooled sample was column-purified (MinElute PCR Purification Kit, Qiagen, UK) and size selection of fragments, c. 320 bp to 590 bp, was performed by agarose gel electrophoresis.

Article Title: CR1-mediated ATP Release by Human Red Blood Cells Promotes CR1 Clustering and Modulates the Immune Transfer Process *
Article Snippet: Ligation of RBC with anti-CD47 was performed according to the protocol for RBC/zymosan ligation at 2% Hct (see below). .. An ATP standard using rATP (Promega) was prepared each day in the following concentrations: 0.5, 1, 5, 10, 50, 100, and 200 n m .

ATP Assay:

Article Title: TPL-2 restricts Ccl24-dependent immunity to Heligmosomoides polygyrus
Article Snippet: Paragraph title: ATP assay for adult H . polygyrus worms ... An ATP standard curve was generated by using recombinant ATP (Promega) as detailed in the CellTiter-Glo Luminescent Cell Viability Assay instructions.

Article Title: Epithelial-Cell-Derived Phospholipase A2 Group 1B Is an Endogenous Anthelmintic
Article Snippet: Paragraph title: ATP Assay ... An ATP standard curve was generated by using recombinant ATP (Promega) as detailed in the CellTiter-Glo® Luminescent Cell Viability Assay instructions.

Infection:

Article Title: Epithelial-Cell-Derived Phospholipase A2 Group 1B Is an Endogenous Anthelmintic
Article Snippet: ATP Assay The ATP of infective L3 H. polygyrus larvae, L4 H. polygyrus larvae (removed from intestinal wall at day 7 post infection) and adult L5 H. polygyrus worms was measured using the CellTiter-Glo® Luminescent Cell Viability Assay (Promega). .. An ATP standard curve was generated by using recombinant ATP (Promega) as detailed in the CellTiter-Glo® Luminescent Cell Viability Assay instructions.

Viability Assay:

Article Title: Transcriptional and Functional Analysis of Bifidobacterium animalis subsp. lactis Exposure to Tetracycline
Article Snippet: Concentrations of ATP were tested with the BacTiter-Glo microbial cell viability assay (p/n G8230; Promega, Madison, WI) on the GlowMax Discover microplate reader (p/n GM3000, Promega) following the manufacturer's instruction. .. Data were generated in relative luminescence units (RLU), which were not back calculated to ATP concentrations, although a standard of 10 mM rATP (p/n P113B; Promega) was added to each run to ensure run-to-run consistency.

Generated:

Article Title: TPL-2 restricts Ccl24-dependent immunity to Heligmosomoides polygyrus
Article Snippet: .. An ATP standard curve was generated by using recombinant ATP (Promega) as detailed in the CellTiter-Glo Luminescent Cell Viability Assay instructions. .. Fecal DNA extraction and 454 sequencing Fecal pellets were homogenized and DNA was extracted using the FastDNA Spin Kit for Soil (MP Biomedicals) as per the manufacturer’s recommendations.

Article Title: Transcriptional and Functional Analysis of Bifidobacterium animalis subsp. lactis Exposure to Tetracycline
Article Snippet: .. Data were generated in relative luminescence units (RLU), which were not back calculated to ATP concentrations, although a standard of 10 mM rATP (p/n P113B; Promega) was added to each run to ensure run-to-run consistency. ..

Article Title: Epithelial-Cell-Derived Phospholipase A2 Group 1B Is an Endogenous Anthelmintic
Article Snippet: .. An ATP standard curve was generated by using recombinant ATP (Promega) as detailed in the CellTiter-Glo® Luminescent Cell Viability Assay instructions. .. Antibiotic Treatment The antibiotics Gentamicin sulfate salt (1 mg/mL, Sigma), Metronidazole (1 mg/mL, Sigma), Cefloxin sodium salt (1 mg/mL, Santa Cruz Biotechnology), Vancomycin hydrochloride (1 mg/mL, Sigma) were administered in the drinking water.

DNA Sequencing:

Article Title: A High Density Genetic Map Derived from RAD Sequencing and Its Application in QTL Analysis of Yield-Related Traits in Vigna unguiculata
Article Snippet: Restriction-site associated DNA (RAD) strategy combined with Illumina DNA sequencing was used for the fast and effective identification of SNP markers. .. The ligation reaction was incubated at room temperature (RT) for 20 min in a final volume of 10 μL containing 2.5 μL P1Adapter (100 nM), 1 μL rATP (100 mM) (Promega), 1 μL 10 × EcoR I buffer, 0.5 μL T4 DNA Ligase (1,000 U) (NEB), and 5 μL H2 O.

Polymerase Chain Reaction:

Article Title: Genetic diversity and structure in Arapaima gigas populations from Amazon and Araguaia-Tocantins river basins
Article Snippet: Ligation was performed over 75 min at 22 °C by addition of a further 2.5 μL of a ligation mix comprising 4 mM rATP (Promega, UK), and 2000 cohesive-end units of T4 ligase (NEB) in 1× CutSmart buffer. .. The reactions were terminated by addition of 20 μL PB buffer (Minelute PCR Purification Kit; Qiagen UK).

Article Title: Species-Specific Marker Discovery in Tilapia
Article Snippet: Ligation was performed over 3.5 h at 22 °C by addition of a further 3 µL of a ligation mix including 4 mM rATP (Promega, UK), and 2000 cohesive-end units of T4 ligase (NEB) in 1× CutSmart buffer. .. The pooled library sample was column-purified (MinElute PCR Purification Kit, Qiagen, UK), and eluted in 80 µL EB buffer (Qiagen, UK).

Article Title: Population genomics of Culiseta melanura, the principal vector of Eastern equine encephalitis virus in the United States
Article Snippet: Digestions were cleaned using AxyPrep Mag PCR Clean-up Kits (Axygen, Union City, CA, USA), hereafter magnetic beads, following manufacturers protocols. .. Adaptors containing sample barcodes were then ligated to each individual sample in a 25 μl reaction with 17 μl of digested DNA, 2.5 μl of 10X T4 DNA Ligase Reaction Buffer (NEB), 1 μl of 10 μM read 1 adaptor, 2 μl of 10 μM read 2 adaptor, 1.5 μl of 10μM rATP (Promega, Madison, WI, USA), and 100 units of T4 DNA Ligase.

Article Title: Gene-centromere mapping in meiotic gynogenetic European seabass
Article Snippet: Ligation was performed over 40 min at 22 °C by addition of a further 3 μL of a ligation mix comprising 4 mM rATP (Promega, UK), and 2000 cohesive-end units of T4 ligase (NEB) in 1× CutSmart buffer. .. The pooled sample was column-purified (MinElute PCR Purification Kit, Qiagen, UK) and size selection of fragments, c. 320 bp to 590 bp, was performed by agarose gel electrophoresis.

Injection:

Article Title: CR1-mediated ATP Release by Human Red Blood Cells Promotes CR1 Clustering and Modulates the Immune Transfer Process *
Article Snippet: ATP release was measured using the GloMax®-Multi+ Detection System (Promega) and ENLITEN® (luciferase/luciferin) reagent, which was injected in equal volumes to the sample into each well and bioluminescence was read. .. An ATP standard using rATP (Promega) was prepared each day in the following concentrations: 0.5, 1, 5, 10, 50, 100, and 200 n m .

Recombinant:

Article Title: TPL-2 restricts Ccl24-dependent immunity to Heligmosomoides polygyrus
Article Snippet: .. An ATP standard curve was generated by using recombinant ATP (Promega) as detailed in the CellTiter-Glo Luminescent Cell Viability Assay instructions. .. Fecal DNA extraction and 454 sequencing Fecal pellets were homogenized and DNA was extracted using the FastDNA Spin Kit for Soil (MP Biomedicals) as per the manufacturer’s recommendations.

Article Title: Epithelial-Cell-Derived Phospholipase A2 Group 1B Is an Endogenous Anthelmintic
Article Snippet: .. An ATP standard curve was generated by using recombinant ATP (Promega) as detailed in the CellTiter-Glo® Luminescent Cell Viability Assay instructions. .. Antibiotic Treatment The antibiotics Gentamicin sulfate salt (1 mg/mL, Sigma), Metronidazole (1 mg/mL, Sigma), Cefloxin sodium salt (1 mg/mL, Santa Cruz Biotechnology), Vancomycin hydrochloride (1 mg/mL, Sigma) were administered in the drinking water.

MTT Assay:

Article Title: Targeting Glycolysis with Epigallocatechin-3-Gallate Enhances the Efficacy of Chemotherapeutics in Pancreatic Cancer Cells and Xenografts
Article Snippet: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (≥97.5%), D-(+)-Glucose (≥99.5%), Oligomycin (≥90%), 2-Deoxy-d -glucose (≥99%), Irinotecan hydrochloride, Oxaliplatin, Catalase, N -Acetyl-l -cysteine (≥99%), DL-Buthionine-sulfoximine (≥99%), RIPA lysis buffer, Halt Protease Inhibitor Cocktail, Phosphatase Inhibitor Cocktail, and the following kits: Phosphofructokinase Activity Colorimetric assay and Pyruvate Kinase Activity assay were purchased from MilliporeSigma (St. Louis, MO, USA). .. CellTiter-Glo® reagent and rATP were purchased from Promega (Madison, WI, USA).

Magnetic Beads:

Article Title: Population genomics of Culiseta melanura, the principal vector of Eastern equine encephalitis virus in the United States
Article Snippet: Digestions were cleaned using AxyPrep Mag PCR Clean-up Kits (Axygen, Union City, CA, USA), hereafter magnetic beads, following manufacturers protocols. .. Adaptors containing sample barcodes were then ligated to each individual sample in a 25 μl reaction with 17 μl of digested DNA, 2.5 μl of 10X T4 DNA Ligase Reaction Buffer (NEB), 1 μl of 10 μM read 1 adaptor, 2 μl of 10 μM read 2 adaptor, 1.5 μl of 10μM rATP (Promega, Madison, WI, USA), and 100 units of T4 DNA Ligase.

Isolation:

Article Title: A High Density Genetic Map Derived from RAD Sequencing and Its Application in QTL Analysis of Yield-Related Traits in Vigna unguiculata
Article Snippet: The ligation reaction was incubated at room temperature (RT) for 20 min in a final volume of 10 μL containing 2.5 μL P1Adapter (100 nM), 1 μL rATP (100 mM) (Promega), 1 μL 10 × EcoR I buffer, 0.5 μL T4 DNA Ligase (1,000 U) (NEB), and 5 μL H2 O. .. The size of sheared DNA was isolated through 1% agarose gel electrophoresis using a DNA gel extraction kit (Tiangen, China).

Mouse Assay:

Article Title: TPL-2 restricts Ccl24-dependent immunity to Heligmosomoides polygyrus
Article Snippet: Briefly, H . polygyrus adult worms were removed from the duodenum of mice and homogenised using a motorised pestle in 110 μL of PBS and 110 μL of CellTiter-Glo Reagent. .. An ATP standard curve was generated by using recombinant ATP (Promega) as detailed in the CellTiter-Glo Luminescent Cell Viability Assay instructions.

Sequencing:

Article Title: Genetic diversity and structure in Arapaima gigas populations from Amazon and Araguaia-Tocantins river basins
Article Snippet: Paragraph title: Library preparation and sequencing ... Ligation was performed over 75 min at 22 °C by addition of a further 2.5 μL of a ligation mix comprising 4 mM rATP (Promega, UK), and 2000 cohesive-end units of T4 ligase (NEB) in 1× CutSmart buffer.

Article Title: Species-Specific Marker Discovery in Tilapia
Article Snippet: Paragraph title: Double Digest RAD library preparation and sequencing ... Ligation was performed over 3.5 h at 22 °C by addition of a further 3 µL of a ligation mix including 4 mM rATP (Promega, UK), and 2000 cohesive-end units of T4 ligase (NEB) in 1× CutSmart buffer.

Article Title: A High Density Genetic Map Derived from RAD Sequencing and Its Application in QTL Analysis of Yield-Related Traits in Vigna unguiculata
Article Snippet: Paragraph title: RAD library construction and sequencing ... The ligation reaction was incubated at room temperature (RT) for 20 min in a final volume of 10 μL containing 2.5 μL P1Adapter (100 nM), 1 μL rATP (100 mM) (Promega), 1 μL 10 × EcoR I buffer, 0.5 μL T4 DNA Ligase (1,000 U) (NEB), and 5 μL H2 O.

Article Title: Gene-centromere mapping in meiotic gynogenetic European seabass
Article Snippet: Paragraph title: ddRAD library preparation and sequencing ... Ligation was performed over 40 min at 22 °C by addition of a further 3 μL of a ligation mix comprising 4 mM rATP (Promega, UK), and 2000 cohesive-end units of T4 ligase (NEB) in 1× CutSmart buffer.

Gel Extraction:

Article Title: Species-Specific Marker Discovery in Tilapia
Article Snippet: Ligation was performed over 3.5 h at 22 °C by addition of a further 3 µL of a ligation mix including 4 mM rATP (Promega, UK), and 2000 cohesive-end units of T4 ligase (NEB) in 1× CutSmart buffer. .. Following gel purification (MinElute Gel Extraction Kit, Qiagen, UK), the eluted size-selected template DNA (60 µL in EB buffer) was PCR amplified (13–14 cycles PCR dependent on library; 24 separate 12.5 µL reactions, each with 1 µL template DNA) using a high fidelity Taq polymerase (Q5 Hot Start High-Fidelity DNA Polymerase, NEB).

Article Title: A High Density Genetic Map Derived from RAD Sequencing and Its Application in QTL Analysis of Yield-Related Traits in Vigna unguiculata
Article Snippet: The ligation reaction was incubated at room temperature (RT) for 20 min in a final volume of 10 μL containing 2.5 μL P1Adapter (100 nM), 1 μL rATP (100 mM) (Promega), 1 μL 10 × EcoR I buffer, 0.5 μL T4 DNA Ligase (1,000 U) (NEB), and 5 μL H2 O. .. The size of sheared DNA was isolated through 1% agarose gel electrophoresis using a DNA gel extraction kit (Tiangen, China).

Article Title: Gene-centromere mapping in meiotic gynogenetic European seabass
Article Snippet: Ligation was performed over 40 min at 22 °C by addition of a further 3 μL of a ligation mix comprising 4 mM rATP (Promega, UK), and 2000 cohesive-end units of T4 ligase (NEB) in 1× CutSmart buffer. .. Following gel purification (MinElute Gel Extraction Kit, Qiagen, UK) the eluted size-selected template DNA (60 μL in EB buffer) was PCR amplified (11 cycles PCR; 28 separate 12.5 μL reactions, each with 1 μL template DNA) using a high fidelity Taq polymerase (Q5 Hot Start High-Fidelity DNA Polymerase, NEB).

Purification:

Article Title: Genetic diversity and structure in Arapaima gigas populations from Amazon and Araguaia-Tocantins river basins
Article Snippet: Ligation was performed over 75 min at 22 °C by addition of a further 2.5 μL of a ligation mix comprising 4 mM rATP (Promega, UK), and 2000 cohesive-end units of T4 ligase (NEB) in 1× CutSmart buffer. .. The reactions were terminated by addition of 20 μL PB buffer (Minelute PCR Purification Kit; Qiagen UK).

Article Title: Species-Specific Marker Discovery in Tilapia
Article Snippet: Ligation was performed over 3.5 h at 22 °C by addition of a further 3 µL of a ligation mix including 4 mM rATP (Promega, UK), and 2000 cohesive-end units of T4 ligase (NEB) in 1× CutSmart buffer. .. The pooled library sample was column-purified (MinElute PCR Purification Kit, Qiagen, UK), and eluted in 80 µL EB buffer (Qiagen, UK).

Article Title: Gene-centromere mapping in meiotic gynogenetic European seabass
Article Snippet: Ligation was performed over 40 min at 22 °C by addition of a further 3 μL of a ligation mix comprising 4 mM rATP (Promega, UK), and 2000 cohesive-end units of T4 ligase (NEB) in 1× CutSmart buffer. .. The pooled sample was column-purified (MinElute PCR Purification Kit, Qiagen, UK) and size selection of fragments, c. 320 bp to 590 bp, was performed by agarose gel electrophoresis.

Article Title: NMDA receptor activity downregulates KCC2 resulting in depolarizing GABAA receptor mediated currents
Article Snippet: .. In vitro phosphorylation of fusion proteins We incubated 0.5 mg of His-C fusion protein with 50 μM rATP (Promega) and 5 ng of purified PKC (EMD Biosciences) in a buffer containing (in mM): 20 HEPES (pH 7.5) and 10 MgCl2 for 10 min at 30 °C and terminated by the addition of 2x SDS-PAGE sample buffer. ..

SDS Page:

Article Title: NMDA receptor activity downregulates KCC2 resulting in depolarizing GABAA receptor mediated currents
Article Snippet: .. In vitro phosphorylation of fusion proteins We incubated 0.5 mg of His-C fusion protein with 50 μM rATP (Promega) and 5 ng of purified PKC (EMD Biosciences) in a buffer containing (in mM): 20 HEPES (pH 7.5) and 10 MgCl2 for 10 min at 30 °C and terminated by the addition of 2x SDS-PAGE sample buffer. ..

Plasmid Preparation:

Article Title: Targeting Glycolysis with Epigallocatechin-3-Gallate Enhances the Efficacy of Chemotherapeutics in Pancreatic Cancer Cells and Xenografts
Article Snippet: CellTiter-Glo® reagent and rATP were purchased from Promega (Madison, WI, USA). .. PFKP siRNA plasmid was purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

Selection:

Article Title: Population genomics of Culiseta melanura, the principal vector of Eastern equine encephalitis virus in the United States
Article Snippet: Library construction was similar to the ‘optimized protocol’ of Hoffberg et al. [ ], although pooling occurred prior to size selection, rather than after adaptor ligation. .. Adaptors containing sample barcodes were then ligated to each individual sample in a 25 μl reaction with 17 μl of digested DNA, 2.5 μl of 10X T4 DNA Ligase Reaction Buffer (NEB), 1 μl of 10 μM read 1 adaptor, 2 μl of 10 μM read 2 adaptor, 1.5 μl of 10μM rATP (Promega, Madison, WI, USA), and 100 units of T4 DNA Ligase.

Article Title: Gene-centromere mapping in meiotic gynogenetic European seabass
Article Snippet: Ligation was performed over 40 min at 22 °C by addition of a further 3 μL of a ligation mix comprising 4 mM rATP (Promega, UK), and 2000 cohesive-end units of T4 ligase (NEB) in 1× CutSmart buffer. .. The pooled sample was column-purified (MinElute PCR Purification Kit, Qiagen, UK) and size selection of fragments, c. 320 bp to 590 bp, was performed by agarose gel electrophoresis.

Agarose Gel Electrophoresis:

Article Title: Species-Specific Marker Discovery in Tilapia
Article Snippet: Ligation was performed over 3.5 h at 22 °C by addition of a further 3 µL of a ligation mix including 4 mM rATP (Promega, UK), and 2000 cohesive-end units of T4 ligase (NEB) in 1× CutSmart buffer. .. Size-selection of fragments, ranging from 320 bp to 590 bp, was performed by agarose gel separation.

Article Title: A High Density Genetic Map Derived from RAD Sequencing and Its Application in QTL Analysis of Yield-Related Traits in Vigna unguiculata
Article Snippet: The ligation reaction was incubated at room temperature (RT) for 20 min in a final volume of 10 μL containing 2.5 μL P1Adapter (100 nM), 1 μL rATP (100 mM) (Promega), 1 μL 10 × EcoR I buffer, 0.5 μL T4 DNA Ligase (1,000 U) (NEB), and 5 μL H2 O. .. The size of sheared DNA was isolated through 1% agarose gel electrophoresis using a DNA gel extraction kit (Tiangen, China).

In Vitro:

Article Title: The extruded non-template strand determines the architecture of R-loops
Article Snippet: .. In vitro transcription of R-loops 1875 ng of circular plasmids were incubated at 37°C with 50 U of T3 RNA Polymerase (Promega) in a transcription buffer (40 mM Tris–HCl pH 7.9, 6 mM MgCl2 , 2 mM spermidine, 10 mM NaCl, 20 mM DTT, 0,05% Tween-20) containing 0,25 mM of rATP, rCTP, rUTP and rGTP (Promega). ..

Article Title: NMDA receptor activity downregulates KCC2 resulting in depolarizing GABAA receptor mediated currents
Article Snippet: .. In vitro phosphorylation of fusion proteins We incubated 0.5 mg of His-C fusion protein with 50 μM rATP (Promega) and 5 ng of purified PKC (EMD Biosciences) in a buffer containing (in mM): 20 HEPES (pH 7.5) and 10 MgCl2 for 10 min at 30 °C and terminated by the addition of 2x SDS-PAGE sample buffer. ..

XF Assay:

Article Title: Targeting Glycolysis with Epigallocatechin-3-Gallate Enhances the Efficacy of Chemotherapeutics in Pancreatic Cancer Cells and Xenografts
Article Snippet: Seahorse XF24 Extracellular Flux assay kits were purchased from Agilent (Santa Clara, CA, USA). .. CellTiter-Glo® reagent and rATP were purchased from Promega (Madison, WI, USA).

Concentration Assay:

Article Title: CR1-mediated ATP Release by Human Red Blood Cells Promotes CR1 Clustering and Modulates the Immune Transfer Process *
Article Snippet: Cell suspension (55 μl) were pipetted into a black 96-well plate and secondary antibody (goat anti-mouse, Jackson, 115-005-003) was added in the same concentration (volume: 5 μl), followed by incubation at room temperature (70 min). .. An ATP standard using rATP (Promega) was prepared each day in the following concentrations: 0.5, 1, 5, 10, 50, 100, and 200 n m .

Article Title: The extruded non-template strand determines the architecture of R-loops
Article Snippet: In vitro transcription of R-loops 1875 ng of circular plasmids were incubated at 37°C with 50 U of T3 RNA Polymerase (Promega) in a transcription buffer (40 mM Tris–HCl pH 7.9, 6 mM MgCl2 , 2 mM spermidine, 10 mM NaCl, 20 mM DTT, 0,05% Tween-20) containing 0,25 mM of rATP, rCTP, rUTP and rGTP (Promega). .. After 140 min at 37°C, the NaCl concentration was brought to 500 mM and 0.4 μg of RNase A was added for 20 min at 37°C to digest soluble RNAs.

Protease Inhibitor:

Article Title: Targeting Glycolysis with Epigallocatechin-3-Gallate Enhances the Efficacy of Chemotherapeutics in Pancreatic Cancer Cells and Xenografts
Article Snippet: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (≥97.5%), D-(+)-Glucose (≥99.5%), Oligomycin (≥90%), 2-Deoxy-d -glucose (≥99%), Irinotecan hydrochloride, Oxaliplatin, Catalase, N -Acetyl-l -cysteine (≥99%), DL-Buthionine-sulfoximine (≥99%), RIPA lysis buffer, Halt Protease Inhibitor Cocktail, Phosphatase Inhibitor Cocktail, and the following kits: Phosphofructokinase Activity Colorimetric assay and Pyruvate Kinase Activity assay were purchased from MilliporeSigma (St. Louis, MO, USA). .. CellTiter-Glo® reagent and rATP were purchased from Promega (Madison, WI, USA).

Lysis:

Article Title: Targeting Glycolysis with Epigallocatechin-3-Gallate Enhances the Efficacy of Chemotherapeutics in Pancreatic Cancer Cells and Xenografts
Article Snippet: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (≥97.5%), D-(+)-Glucose (≥99.5%), Oligomycin (≥90%), 2-Deoxy-d -glucose (≥99%), Irinotecan hydrochloride, Oxaliplatin, Catalase, N -Acetyl-l -cysteine (≥99%), DL-Buthionine-sulfoximine (≥99%), RIPA lysis buffer, Halt Protease Inhibitor Cocktail, Phosphatase Inhibitor Cocktail, and the following kits: Phosphofructokinase Activity Colorimetric assay and Pyruvate Kinase Activity assay were purchased from MilliporeSigma (St. Louis, MO, USA). .. CellTiter-Glo® reagent and rATP were purchased from Promega (Madison, WI, USA).

Cell Viability Assay:

Article Title: TPL-2 restricts Ccl24-dependent immunity to Heligmosomoides polygyrus
Article Snippet: .. An ATP standard curve was generated by using recombinant ATP (Promega) as detailed in the CellTiter-Glo Luminescent Cell Viability Assay instructions. .. Fecal DNA extraction and 454 sequencing Fecal pellets were homogenized and DNA was extracted using the FastDNA Spin Kit for Soil (MP Biomedicals) as per the manufacturer’s recommendations.

Article Title: Epithelial-Cell-Derived Phospholipase A2 Group 1B Is an Endogenous Anthelmintic
Article Snippet: .. An ATP standard curve was generated by using recombinant ATP (Promega) as detailed in the CellTiter-Glo® Luminescent Cell Viability Assay instructions. .. Antibiotic Treatment The antibiotics Gentamicin sulfate salt (1 mg/mL, Sigma), Metronidazole (1 mg/mL, Sigma), Cefloxin sodium salt (1 mg/mL, Santa Cruz Biotechnology), Vancomycin hydrochloride (1 mg/mL, Sigma) were administered in the drinking water.

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    Promega ratp standard
    Ratp Standard, supplied by Promega, used in various techniques. Bioz Stars score: 78/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Ratp, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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