Journal: Neural Regeneration Research

Article Title: Polyethylene glycol has immunoprotective effects on sciatic allografts, but behavioral recovery and graft tolerance require neurorrhaphy and axonal fusion

doi: 10.4103/NRR.NRR-D-23-01220

Figure Lengend Snippet: Historical data vs . data collected for LS-PEG PNA study

Article Snippet: Primary antibodies included rabbit anti-rat CD3 (1:100; Abcam, Waltham, MA, USA, Cat# ab828, RRID: AB_306429), mouse anti-rat CD8a (1:100; Invitrogen, Cat# 14-0084-82, RRID: AB_1210523), rabbit anti-rat CD11B (1:400; Novus Biologicals, Chesterfield, MO, USA, Cat# NB110-89474, RRID: AB_1216361), rabbit anti-rat CD206 (1:400; Proteintech, Rosemont, IL, USA, Cat# 18704-1-AP, RRID: AB_10597232), mouse anti-rat CD68 (1:200; Bio-Rad, Hercules, CA, USA, Cat# MCA341GA, RRID: AB_566872;), mouse anti-rat MHCI (1:100; Novus Biologicals, Cat# NB120- 6405, RRID: AB_791151), mouse anti-rat MHCII (1:100; Bio-Rad, Cat# MCA46GA; RRID: AB_567369), mouse anti-rat neurofilament-medium (NF-M; 1:200; DSHB, Iowa City, IA, USA, Cat# 2H3, RRID: AB_531793), rabbit anti-rat myelin basic protein (MBP; 1:200; Bio-Rad, Cat# ab40390, RRID: AB_1141521), rabbit anti-rat cleaved-Caspase 3 (1:200; Cell Signaling Technologies, Danvers, MA, USA, Cat# CST9661, RRID: AB_2341188), rat anti-rat FoxP3-eFluor 660 conjugate (1:100; eBioscience, Waltham, MA, USA, Cat# 50-5773-82, RRID: AB_11218868) and mouse anti-rat CD4-PE conjugate (1:100; eBioscience, Cat# 12-0040-82, RRID: AB_2572548).

Techniques: Generated, Functional Assay

Journal: Neural Regeneration Research

Article Title: Polyethylene glycol has immunoprotective effects on sciatic allografts, but behavioral recovery and graft tolerance require neurorrhaphy and axonal fusion

doi: 10.4103/NRR.NRR-D-23-01220

Figure Lengend Snippet: Immunohistochemical and genetic analyses of FoxP3 + /CD4 + Treg infiltration in PNAs. (A, B) Confocal fluorescent images of nerve cross-sections immunostained for Tregs (Foxp3, magenta; CD4, green). Scale bar: 50 µm. (C) Quantification of integrated density (%) of double-labeled FoxP3/CD4 Tregs. Two-way ANOVA (α) F (4, 9) = 1.76, ns, (β) F (2, 9) = 5.62, p = 0.0040, (γ) F (2, 9) = 2.07, ns. (D) Quantification of FOXP3 mRNA expression at 14 days PO. One-way ANOVA (δ) F (2, 9) = 1.82, ns. (E) Quantification of transforming growth factor-β (TGF-β1) mRNA expression at 14 days PO. One-way ANOVA (δ) F (2, 9) = 1.53, ns. Tukey’s post hoc test was used. * P < 0.05. ANOVA: Analysis of variance; LS: loose-sutured; NC: negative control; ns: not significant; PEG: polyethylene glycol fusion; PNAs: peripheral nerve allografts; PO: postoperatively.

Article Snippet: Primary antibodies included rabbit anti-rat CD3 (1:100; Abcam, Waltham, MA, USA, Cat# ab828, RRID: AB_306429), mouse anti-rat CD8a (1:100; Invitrogen, Cat# 14-0084-82, RRID: AB_1210523), rabbit anti-rat CD11B (1:400; Novus Biologicals, Chesterfield, MO, USA, Cat# NB110-89474, RRID: AB_1216361), rabbit anti-rat CD206 (1:400; Proteintech, Rosemont, IL, USA, Cat# 18704-1-AP, RRID: AB_10597232), mouse anti-rat CD68 (1:200; Bio-Rad, Hercules, CA, USA, Cat# MCA341GA, RRID: AB_566872;), mouse anti-rat MHCI (1:100; Novus Biologicals, Cat# NB120- 6405, RRID: AB_791151), mouse anti-rat MHCII (1:100; Bio-Rad, Cat# MCA46GA; RRID: AB_567369), mouse anti-rat neurofilament-medium (NF-M; 1:200; DSHB, Iowa City, IA, USA, Cat# 2H3, RRID: AB_531793), rabbit anti-rat myelin basic protein (MBP; 1:200; Bio-Rad, Cat# ab40390, RRID: AB_1141521), rabbit anti-rat cleaved-Caspase 3 (1:200; Cell Signaling Technologies, Danvers, MA, USA, Cat# CST9661, RRID: AB_2341188), rat anti-rat FoxP3-eFluor 660 conjugate (1:100; eBioscience, Waltham, MA, USA, Cat# 50-5773-82, RRID: AB_11218868) and mouse anti-rat CD4-PE conjugate (1:100; eBioscience, Cat# 12-0040-82, RRID: AB_2572548).

Techniques: Immunohistochemical staining, Labeling, Expressing, Negative Control