rat trpv1  (Alomone Labs)


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    Structured Review

    Alomone Labs rat trpv1
    Photomicrographs of cryosections of canine skin showing transient receptor potential vanilloid 1 <t>(TRPV1)</t> immunoreactivity (IR) in the tissues of the healthy dogs (CTRL) (a–c) and in the dogs with atopic dermatitis (AD) (d–f) . (a–c) The white arrows indicate some DAPI-labeled nuclei of the keratinocytes of the CTRL dogs showing moderate cytoplasmic <t>TRPV1-IR.</t> The open arrows indicate some DAPI-labeled nuclei of the dermal cells showing moderate TRPV1-IR. (d–f) The white arrows indicate some DAPI-labeled nuclei of the keratinocytes of the AD dogs showing bright cytoplasmic TRPV1-IR. The open arrows indicate some DAPI-labeled nuclei of the dermal cells showing bright TRPV1-IR. Bar, 50 μm.
    Rat Trpv1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rat trpv1/product/Alomone Labs
    Average 94 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    rat trpv1 - by Bioz Stars, 2022-09
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    Images

    1) Product Images from "Distribution of Cannabinoid Receptors in Keratinocytes of Healthy Dogs and Dogs With Atopic Dermatitis"

    Article Title: Distribution of Cannabinoid Receptors in Keratinocytes of Healthy Dogs and Dogs With Atopic Dermatitis

    Journal: Frontiers in Veterinary Science

    doi: 10.3389/fvets.2022.915896

    Photomicrographs of cryosections of canine skin showing transient receptor potential vanilloid 1 (TRPV1) immunoreactivity (IR) in the tissues of the healthy dogs (CTRL) (a–c) and in the dogs with atopic dermatitis (AD) (d–f) . (a–c) The white arrows indicate some DAPI-labeled nuclei of the keratinocytes of the CTRL dogs showing moderate cytoplasmic TRPV1-IR. The open arrows indicate some DAPI-labeled nuclei of the dermal cells showing moderate TRPV1-IR. (d–f) The white arrows indicate some DAPI-labeled nuclei of the keratinocytes of the AD dogs showing bright cytoplasmic TRPV1-IR. The open arrows indicate some DAPI-labeled nuclei of the dermal cells showing bright TRPV1-IR. Bar, 50 μm.
    Figure Legend Snippet: Photomicrographs of cryosections of canine skin showing transient receptor potential vanilloid 1 (TRPV1) immunoreactivity (IR) in the tissues of the healthy dogs (CTRL) (a–c) and in the dogs with atopic dermatitis (AD) (d–f) . (a–c) The white arrows indicate some DAPI-labeled nuclei of the keratinocytes of the CTRL dogs showing moderate cytoplasmic TRPV1-IR. The open arrows indicate some DAPI-labeled nuclei of the dermal cells showing moderate TRPV1-IR. (d–f) The white arrows indicate some DAPI-labeled nuclei of the keratinocytes of the AD dogs showing bright cytoplasmic TRPV1-IR. The open arrows indicate some DAPI-labeled nuclei of the dermal cells showing bright TRPV1-IR. Bar, 50 μm.

    Techniques Used: Labeling

    Quantification of the intensity of the expression of CB1R (a) , CB2R (b) , GPR55 (c) , PPARα (d) , TRPV1 (e) , TRPA1 (f) , 5-HT1aR (g) , in the suprabasal layers of 7 CTRL- and 8 AD-dogs. Data are represented as Mean ± SD and were analyzed using the Student T -test. P
    Figure Legend Snippet: Quantification of the intensity of the expression of CB1R (a) , CB2R (b) , GPR55 (c) , PPARα (d) , TRPV1 (e) , TRPA1 (f) , 5-HT1aR (g) , in the suprabasal layers of 7 CTRL- and 8 AD-dogs. Data are represented as Mean ± SD and were analyzed using the Student T -test. P

    Techniques Used: Expressing

    Photomicrographs of cryosections of canine skin showing transient receptor potential ankyrin 1 (TRPA1) immunoreactivity (IR) in the tissues of the healthy dogs (CTRL) (a–c) and in the dogs with atopic dermatitis (AD) (d–f) . (a–c) The white arrows indicate some DAPI-labeled nuclei of the keratinocytes of the CTRL dogs showing moderate cytoplasmic TRPV1-IR. The TRPA1 immunolabelling was brighter in the more superficial cells. The open arrows indicate some DAPI-labeled nuclei of the dermal cells showing moderate TRPV1-IR. (d–f) The white arrows indicate some DAPI-labeled nuclei of the keratinocytes of the AD dogs showing bright cytoplasmic TRPV1-IR. Not all the cells of the suprabasal layer showed the same degree of TRPA1-IR which was brighter in the cytoplasm of the more superficial cells. The open arrows indicate some DAPI-labeled nuclei of the dermal cells showing bright TRPA1-IR. Bar, 50 μm.
    Figure Legend Snippet: Photomicrographs of cryosections of canine skin showing transient receptor potential ankyrin 1 (TRPA1) immunoreactivity (IR) in the tissues of the healthy dogs (CTRL) (a–c) and in the dogs with atopic dermatitis (AD) (d–f) . (a–c) The white arrows indicate some DAPI-labeled nuclei of the keratinocytes of the CTRL dogs showing moderate cytoplasmic TRPV1-IR. The TRPA1 immunolabelling was brighter in the more superficial cells. The open arrows indicate some DAPI-labeled nuclei of the dermal cells showing moderate TRPV1-IR. (d–f) The white arrows indicate some DAPI-labeled nuclei of the keratinocytes of the AD dogs showing bright cytoplasmic TRPV1-IR. Not all the cells of the suprabasal layer showed the same degree of TRPA1-IR which was brighter in the cytoplasm of the more superficial cells. The open arrows indicate some DAPI-labeled nuclei of the dermal cells showing bright TRPA1-IR. Bar, 50 μm.

    Techniques Used: Labeling

    2) Product Images from "Capsaicin activates TRPV1 in human Langerhans cells and inhibits mucosal HIV-1 transmission via secreted CGRP"

    Article Title: Capsaicin activates TRPV1 in human Langerhans cells and inhibits mucosal HIV-1 transmission via secreted CGRP

    Journal: bioRxiv

    doi: 10.1101/2021.03.08.434408

    LCs express functional TRPV1. (A) Relative TRPV1 mRNA expression in MDLCs from three different human individuals ( 1 – 3 ) normalized to beta actin, with total human brain (HB) mRNA serving as positive control. (B, C) MDLCs (B) or epidermal cell suspensions (C) were stained for surface langerin and either intracellular or extracellular TRPV1 expression as indicated, and examined by flow cytometry. Representative overlays show TRPV1 expression (grey histograms) on langerin + gated MDLCs (B) or epidermal cells (C, middle and left), as well as on langerin neg epidermal cells (C, middle and right) vs. matched isotype controls (line histograms). To validate signal specificity, the intracellular TRPV1 Ab was pre-incubated with a blocking peptide before staining (B, left; broken line histogram). Numbers represent mean±SEM percentages of positive cells, derived from n=6 (B, left), n=4 (B, right) and n=3 (C) experiments, using MDLCs or foreskin tissues from different individuals. (D) MDLCs were loaded with the Ca 2+ indicator Indo-1 and examined over time by flow cytometry. Representative graphs (n=3) show the normalized bound/free Ca 2+ ratio, indicative of Ca 2+ influx, at baseline (t=0sec, set to 1) and following treatment (t=60sec, arrow) with the indicated concentrations of CP (left) or RTX (right). Ionomycin (1μM; broken lines) served as positive control.
    Figure Legend Snippet: LCs express functional TRPV1. (A) Relative TRPV1 mRNA expression in MDLCs from three different human individuals ( 1 – 3 ) normalized to beta actin, with total human brain (HB) mRNA serving as positive control. (B, C) MDLCs (B) or epidermal cell suspensions (C) were stained for surface langerin and either intracellular or extracellular TRPV1 expression as indicated, and examined by flow cytometry. Representative overlays show TRPV1 expression (grey histograms) on langerin + gated MDLCs (B) or epidermal cells (C, middle and left), as well as on langerin neg epidermal cells (C, middle and right) vs. matched isotype controls (line histograms). To validate signal specificity, the intracellular TRPV1 Ab was pre-incubated with a blocking peptide before staining (B, left; broken line histogram). Numbers represent mean±SEM percentages of positive cells, derived from n=6 (B, left), n=4 (B, right) and n=3 (C) experiments, using MDLCs or foreskin tissues from different individuals. (D) MDLCs were loaded with the Ca 2+ indicator Indo-1 and examined over time by flow cytometry. Representative graphs (n=3) show the normalized bound/free Ca 2+ ratio, indicative of Ca 2+ influx, at baseline (t=0sec, set to 1) and following treatment (t=60sec, arrow) with the indicated concentrations of CP (left) or RTX (right). Ionomycin (1μM; broken lines) served as positive control.

    Techniques Used: Functional Assay, Expressing, Positive Control, Staining, Flow Cytometry, Incubation, Blocking Assay, Derivative Assay

    TRPV1 activation in MDLCs inhibits HIV-1 trans-infection via secreted CGRP. (A, B) MDLCs were treated for 24h with the indicated molar concentrations of CGRP or CP. The TRPV1 antagonist A425619 was added 15min before addition of CP. The cells were then pulsed with HIV-1 for 4h, washed, and co-cultured with autologous CD4+ T-cells. HIV-1 replication was evaluated a week later by measuring p24 content in the co-culture supernatants using ELISA. In (A), shown are mean±SEM (n=3) percentages of HIV-1 trans-infection inhibition, calculated against untreated cells (i.e. no inhibition). Extrapolated IC 50 values were 3.4×10 −11 M for CGRP and 1.6×10 −6 M for CP. In (B), shown are mean±SEM (n=4) percentages of HIV-1 trans-infection, normalized against untreated cells serving as the 100% set point. Treatment with 0.1% ethanol (EtOH) served as control. (C) MDLCs were treated for 24h with the indicated molar concentrations CGRP, CP or Rut. Culture supernatants were collected immediately after CP and Rut treatment, or following extensive washing and culture in fresh medium for addition 24h after CGRP treatment, and an EIA was used to measure CGRP levels. Shown are mean±SEM (n=3) levels of secreted CGRP per 10 6 MDLCs. (D) MDLCs were treated for 24h with CGRP or CP. The CGRP-R antagonist BIBN4096 (BIBN) was added 15min before addition of agonists. HIV-1 trans-infection was evaluated as above and results (n=4) are shown as in (B). In all graphs, *p
    Figure Legend Snippet: TRPV1 activation in MDLCs inhibits HIV-1 trans-infection via secreted CGRP. (A, B) MDLCs were treated for 24h with the indicated molar concentrations of CGRP or CP. The TRPV1 antagonist A425619 was added 15min before addition of CP. The cells were then pulsed with HIV-1 for 4h, washed, and co-cultured with autologous CD4+ T-cells. HIV-1 replication was evaluated a week later by measuring p24 content in the co-culture supernatants using ELISA. In (A), shown are mean±SEM (n=3) percentages of HIV-1 trans-infection inhibition, calculated against untreated cells (i.e. no inhibition). Extrapolated IC 50 values were 3.4×10 −11 M for CGRP and 1.6×10 −6 M for CP. In (B), shown are mean±SEM (n=4) percentages of HIV-1 trans-infection, normalized against untreated cells serving as the 100% set point. Treatment with 0.1% ethanol (EtOH) served as control. (C) MDLCs were treated for 24h with the indicated molar concentrations CGRP, CP or Rut. Culture supernatants were collected immediately after CP and Rut treatment, or following extensive washing and culture in fresh medium for addition 24h after CGRP treatment, and an EIA was used to measure CGRP levels. Shown are mean±SEM (n=3) levels of secreted CGRP per 10 6 MDLCs. (D) MDLCs were treated for 24h with CGRP or CP. The CGRP-R antagonist BIBN4096 (BIBN) was added 15min before addition of agonists. HIV-1 trans-infection was evaluated as above and results (n=4) are shown as in (B). In all graphs, *p

    Techniques Used: Activation Assay, Infection, Cell Culture, Co-Culture Assay, Enzyme-linked Immunosorbent Assay, Inhibition

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    Alomone Labs rabbit anti trpv1
    Harpagophytum procumbens (HP) prevents upregulation of <t>TRPV1</t> in dorsal root ganglions (DRG) and attenuates chronic neuropathic pain. (a) Representative immunohistochemical images of TRPV1 (green) and NeuN (red) in DRGs at 3 weeks after LSS in each group. White scale bar = 200 μ m. (b) Percentage of TRPV1 + neurons that colabeled with NeuN + neurons in DRG. (c) Quantification of fluorescence intensity in TRPV1 + neuron within DRG. Data are expressed as the means ± SEM. Significant differences are indicated as #### p
    Rabbit Anti Trpv1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Alomone Labs anti trpv1 vr1 antibody
    STZ injection upregulated the expression of <t>TRPV1</t> in DRG, whereas ALA downregulated TRPV1 expression. A, Western blots for TRPV1 of DRGs innervating the hindpaw from control and STZ injection rats. Bar graph indicated mean density relative of glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) and TRPV1 from CNT and STZ rats. STZ injection significantly enhanced expression of TRPV1 of DRGs innervating the hindpaw (N = 4 for each group, * P
    Anti Trpv1 Vr1 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti trpv1 vr1 antibody/product/Alomone Labs
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    93
    Alomone Labs rabbit anti trpv1 antibody
    IAN transection both in NP and non-NP groups changes the expression profile of <t>TRPV1</t> to myelinated neurons of a larger diameter. The total number of TG cells labeled for the fluoro-gold (FG + ) (A: NP group, B: Non-NP group); TG cells that labeled for TRPV1 and FG (TRPV1 + +FG + ) in 2-; 3-and 4-week NP groups and in sham-operated group (C: NP group, D: Non-NP group). The ratio of TRPV1 + +FG + to all FG+ positive cells (E: NP group, F: Non-NP group). n = 5 for each group, (ANOVA followed by the Student–Newman–Keuls test, *p
    Rabbit Anti Trpv1 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti trpv1 antibody/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti trpv1 antibody - by Bioz Stars, 2022-09
    93/100 stars
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    94
    Alomone Labs rat trpv1
    Photomicrographs of cryosections of canine skin showing transient receptor potential vanilloid 1 <t>(TRPV1)</t> immunoreactivity (IR) in the tissues of the healthy dogs (CTRL) (a–c) and in the dogs with atopic dermatitis (AD) (d–f) . (a–c) The white arrows indicate some DAPI-labeled nuclei of the keratinocytes of the CTRL dogs showing moderate cytoplasmic <t>TRPV1-IR.</t> The open arrows indicate some DAPI-labeled nuclei of the dermal cells showing moderate TRPV1-IR. (d–f) The white arrows indicate some DAPI-labeled nuclei of the keratinocytes of the AD dogs showing bright cytoplasmic TRPV1-IR. The open arrows indicate some DAPI-labeled nuclei of the dermal cells showing bright TRPV1-IR. Bar, 50 μm.
    Rat Trpv1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rat trpv1/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rat trpv1 - by Bioz Stars, 2022-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    Harpagophytum procumbens (HP) prevents upregulation of TRPV1 in dorsal root ganglions (DRG) and attenuates chronic neuropathic pain. (a) Representative immunohistochemical images of TRPV1 (green) and NeuN (red) in DRGs at 3 weeks after LSS in each group. White scale bar = 200 μ m. (b) Percentage of TRPV1 + neurons that colabeled with NeuN + neurons in DRG. (c) Quantification of fluorescence intensity in TRPV1 + neuron within DRG. Data are expressed as the means ± SEM. Significant differences are indicated as #### p

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Harpagophytum procumbens Inhibits Iron Overload-Induced Oxidative Stress through Activation of Nrf2 Signaling in a Rat Model of Lumbar Spinal Stenosis

    doi: 10.1155/2022/3472443

    Figure Lengend Snippet: Harpagophytum procumbens (HP) prevents upregulation of TRPV1 in dorsal root ganglions (DRG) and attenuates chronic neuropathic pain. (a) Representative immunohistochemical images of TRPV1 (green) and NeuN (red) in DRGs at 3 weeks after LSS in each group. White scale bar = 200 μ m. (b) Percentage of TRPV1 + neurons that colabeled with NeuN + neurons in DRG. (c) Quantification of fluorescence intensity in TRPV1 + neuron within DRG. Data are expressed as the means ± SEM. Significant differences are indicated as #### p

    Article Snippet: Sections were incubated with primary antibodies against rabbit anti-CD68 (Abcam, 1 : 500), rabbit anti-TRPV1 (Alomone, Hadassah Ein Kerem, Israel, 1 : 100), guinea pig anti-NeuN (Synaptic Systems, Gottingen, Germany, 1 : 500), mouse antiferritin heavy chain (Santa Cruz Biotechnology, Santa Cruz, CA, USA, 1 : 400), mouse anti-iNOS (R & D Systems, Minneapolis, MN, USA, 1 : 100), rabbit anti-NRF2 (Abcam, 1 : 200), and mouse anti-NF200 (Millipore, Billerica, MA, USA, 1 : 200) overnight at 4°C.

    Techniques: Immunohistochemistry, Fluorescence

    STZ injection upregulated the expression of TRPV1 in DRG, whereas ALA downregulated TRPV1 expression. A, Western blots for TRPV1 of DRGs innervating the hindpaw from control and STZ injection rats. Bar graph indicated mean density relative of glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) and TRPV1 from CNT and STZ rats. STZ injection significantly enhanced expression of TRPV1 of DRGs innervating the hindpaw (N = 4 for each group, * P

    Journal: CNS Neuroscience & Therapeutics

    Article Title: Alpha‐lipoic acid downregulates TRPV1 receptor via NF‐κB and attenuates neuropathic pain in rats with diabetes, et al. Alpha‐lipoic acid downregulates TRPV1 receptor via NF‐κB and attenuates neuropathic pain in rats with diabetes

    doi: 10.1111/cns.13303

    Figure Lengend Snippet: STZ injection upregulated the expression of TRPV1 in DRG, whereas ALA downregulated TRPV1 expression. A, Western blots for TRPV1 of DRGs innervating the hindpaw from control and STZ injection rats. Bar graph indicated mean density relative of glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) and TRPV1 from CNT and STZ rats. STZ injection significantly enhanced expression of TRPV1 of DRGs innervating the hindpaw (N = 4 for each group, * P

    Article Snippet: Mouse anti‐p65 (#6956, Cell Signaling Technology, 1:50) and rabbit anti‐TRPV1 (#ACC‐030, Alomone, 1:50) were used in this study.

    Techniques: Injection, Expressing, Western Blot

    TRPV1 and NF‐κB were co‐expressed in DRG neurons. A, NF‐κB‐positive cells were shown in red. B, TRPV1‐positive cells were shown in green. C, Merge of double labeling of TRPV1 and NF‐κB. Scale bar was 50 μm. D, Quantified analysis showed the majority of TRPV1 was co‐expressed with NF‐κB‐positive DRG neurons, and the majority of NF‐κB was also co‐expressed with TRPV1‐positive DRG neurons

    Journal: CNS Neuroscience & Therapeutics

    Article Title: Alpha‐lipoic acid downregulates TRPV1 receptor via NF‐κB and attenuates neuropathic pain in rats with diabetes, et al. Alpha‐lipoic acid downregulates TRPV1 receptor via NF‐κB and attenuates neuropathic pain in rats with diabetes

    doi: 10.1111/cns.13303

    Figure Lengend Snippet: TRPV1 and NF‐κB were co‐expressed in DRG neurons. A, NF‐κB‐positive cells were shown in red. B, TRPV1‐positive cells were shown in green. C, Merge of double labeling of TRPV1 and NF‐κB. Scale bar was 50 μm. D, Quantified analysis showed the majority of TRPV1 was co‐expressed with NF‐κB‐positive DRG neurons, and the majority of NF‐κB was also co‐expressed with TRPV1‐positive DRG neurons

    Article Snippet: Mouse anti‐p65 (#6956, Cell Signaling Technology, 1:50) and rabbit anti‐TRPV1 (#ACC‐030, Alomone, 1:50) were used in this study.

    Techniques: Labeling

    ALA inhibited TRPV1 expression via suppressing NF‐κB. A, Western blots for NF‐κB in DRGs innervating the hindpaw from control and STZ‐induced diabetic rats. Bar graph involved mean density relative of β‐actin and NF‐κB from control and STZ‐induced diabetic rats. STZ injection significantly enhanced expression of NF‐κB (N = 4 for each group; * P

    Journal: CNS Neuroscience & Therapeutics

    Article Title: Alpha‐lipoic acid downregulates TRPV1 receptor via NF‐κB and attenuates neuropathic pain in rats with diabetes, et al. Alpha‐lipoic acid downregulates TRPV1 receptor via NF‐κB and attenuates neuropathic pain in rats with diabetes

    doi: 10.1111/cns.13303

    Figure Lengend Snippet: ALA inhibited TRPV1 expression via suppressing NF‐κB. A, Western blots for NF‐κB in DRGs innervating the hindpaw from control and STZ‐induced diabetic rats. Bar graph involved mean density relative of β‐actin and NF‐κB from control and STZ‐induced diabetic rats. STZ injection significantly enhanced expression of NF‐κB (N = 4 for each group; * P

    Article Snippet: Mouse anti‐p65 (#6956, Cell Signaling Technology, 1:50) and rabbit anti‐TRPV1 (#ACC‐030, Alomone, 1:50) were used in this study.

    Techniques: Expressing, Western Blot, Injection

    IAN transection both in NP and non-NP groups changes the expression profile of TRPV1 to myelinated neurons of a larger diameter. The total number of TG cells labeled for the fluoro-gold (FG + ) (A: NP group, B: Non-NP group); TG cells that labeled for TRPV1 and FG (TRPV1 + +FG + ) in 2-; 3-and 4-week NP groups and in sham-operated group (C: NP group, D: Non-NP group). The ratio of TRPV1 + +FG + to all FG+ positive cells (E: NP group, F: Non-NP group). n = 5 for each group, (ANOVA followed by the Student–Newman–Keuls test, *p

    Journal: PLoS ONE

    Article Title: Expression of TRPV1 Channels after Nerve Injury Provides an Essential Delivery Tool for Neuropathic Pain Attenuation

    doi: 10.1371/journal.pone.0044023

    Figure Lengend Snippet: IAN transection both in NP and non-NP groups changes the expression profile of TRPV1 to myelinated neurons of a larger diameter. The total number of TG cells labeled for the fluoro-gold (FG + ) (A: NP group, B: Non-NP group); TG cells that labeled for TRPV1 and FG (TRPV1 + +FG + ) in 2-; 3-and 4-week NP groups and in sham-operated group (C: NP group, D: Non-NP group). The ratio of TRPV1 + +FG + to all FG+ positive cells (E: NP group, F: Non-NP group). n = 5 for each group, (ANOVA followed by the Student–Newman–Keuls test, *p

    Article Snippet: They were then coincubated overnight at 4°C with a combination of rabbit anti-TRPV1 antibody (1∶200; Alomone Labs Ltd., Israel), which was diluted with 3% NGS in 0.01 M PBS with 0.3% Triton X-100, and mouse monoclonal anti-neurofilament 200 (NF200) antibody (1∶1000; Sigma-Aldrich), which was diluted with 3% NGS in 0.01 M PBS with 0.3% Triton X-100.

    Techniques: Expressing, Labeling

    Photomicrographs of immunohistochemistry of TG cells labeled for TRPV1, NF200 and FG in sham-operated group and in 2-; 3-and 4-week NP groups and in 2-; 3- and 4 weeks non-NP groups. Expanded view of TG in the sham-operated group (D1–D4). Arrow points on an example of TRPV1 + +FG + +NF - cell. Arrowhead points on an example of TRPV1 + +FG + +NF + cell. Note that TRPV1-positive cells increased with time after transection. Scale bar: 50 µm.

    Journal: PLoS ONE

    Article Title: Expression of TRPV1 Channels after Nerve Injury Provides an Essential Delivery Tool for Neuropathic Pain Attenuation

    doi: 10.1371/journal.pone.0044023

    Figure Lengend Snippet: Photomicrographs of immunohistochemistry of TG cells labeled for TRPV1, NF200 and FG in sham-operated group and in 2-; 3-and 4-week NP groups and in 2-; 3- and 4 weeks non-NP groups. Expanded view of TG in the sham-operated group (D1–D4). Arrow points on an example of TRPV1 + +FG + +NF - cell. Arrowhead points on an example of TRPV1 + +FG + +NF + cell. Note that TRPV1-positive cells increased with time after transection. Scale bar: 50 µm.

    Article Snippet: They were then coincubated overnight at 4°C with a combination of rabbit anti-TRPV1 antibody (1∶200; Alomone Labs Ltd., Israel), which was diluted with 3% NGS in 0.01 M PBS with 0.3% Triton X-100, and mouse monoclonal anti-neurofilament 200 (NF200) antibody (1∶1000; Sigma-Aldrich), which was diluted with 3% NGS in 0.01 M PBS with 0.3% Triton X-100.

    Techniques: Immunohistochemistry, Labeling

    The pattern of distribution of TRPV1 was altered in non-NP groups. The distribution area of TRPV1 + +FG + +NF + positive cells for all experimental groups. A cell area > 1000 µm 2 was considered large, while that

    Journal: PLoS ONE

    Article Title: Expression of TRPV1 Channels after Nerve Injury Provides an Essential Delivery Tool for Neuropathic Pain Attenuation

    doi: 10.1371/journal.pone.0044023

    Figure Lengend Snippet: The pattern of distribution of TRPV1 was altered in non-NP groups. The distribution area of TRPV1 + +FG + +NF + positive cells for all experimental groups. A cell area > 1000 µm 2 was considered large, while that

    Article Snippet: They were then coincubated overnight at 4°C with a combination of rabbit anti-TRPV1 antibody (1∶200; Alomone Labs Ltd., Israel), which was diluted with 3% NGS in 0.01 M PBS with 0.3% Triton X-100, and mouse monoclonal anti-neurofilament 200 (NF200) antibody (1∶1000; Sigma-Aldrich), which was diluted with 3% NGS in 0.01 M PBS with 0.3% Triton X-100.

    Techniques:

    Photomicrographs of cryosections of canine skin showing transient receptor potential vanilloid 1 (TRPV1) immunoreactivity (IR) in the tissues of the healthy dogs (CTRL) (a–c) and in the dogs with atopic dermatitis (AD) (d–f) . (a–c) The white arrows indicate some DAPI-labeled nuclei of the keratinocytes of the CTRL dogs showing moderate cytoplasmic TRPV1-IR. The open arrows indicate some DAPI-labeled nuclei of the dermal cells showing moderate TRPV1-IR. (d–f) The white arrows indicate some DAPI-labeled nuclei of the keratinocytes of the AD dogs showing bright cytoplasmic TRPV1-IR. The open arrows indicate some DAPI-labeled nuclei of the dermal cells showing bright TRPV1-IR. Bar, 50 μm.

    Journal: Frontiers in Veterinary Science

    Article Title: Distribution of Cannabinoid Receptors in Keratinocytes of Healthy Dogs and Dogs With Atopic Dermatitis

    doi: 10.3389/fvets.2022.915896

    Figure Lengend Snippet: Photomicrographs of cryosections of canine skin showing transient receptor potential vanilloid 1 (TRPV1) immunoreactivity (IR) in the tissues of the healthy dogs (CTRL) (a–c) and in the dogs with atopic dermatitis (AD) (d–f) . (a–c) The white arrows indicate some DAPI-labeled nuclei of the keratinocytes of the CTRL dogs showing moderate cytoplasmic TRPV1-IR. The open arrows indicate some DAPI-labeled nuclei of the dermal cells showing moderate TRPV1-IR. (d–f) The white arrows indicate some DAPI-labeled nuclei of the keratinocytes of the AD dogs showing bright cytoplasmic TRPV1-IR. The open arrows indicate some DAPI-labeled nuclei of the dermal cells showing bright TRPV1-IR. Bar, 50 μm.

    Article Snippet: The immunogen of the rabbit anti-TRPV1 (ACC-030) was the peptide (C)EDAEVFK DSMVPGEK (824–838) of rat TRPV1.

    Techniques: Labeling

    Quantification of the intensity of the expression of CB1R (a) , CB2R (b) , GPR55 (c) , PPARα (d) , TRPV1 (e) , TRPA1 (f) , 5-HT1aR (g) , in the suprabasal layers of 7 CTRL- and 8 AD-dogs. Data are represented as Mean ± SD and were analyzed using the Student T -test. P

    Journal: Frontiers in Veterinary Science

    Article Title: Distribution of Cannabinoid Receptors in Keratinocytes of Healthy Dogs and Dogs With Atopic Dermatitis

    doi: 10.3389/fvets.2022.915896

    Figure Lengend Snippet: Quantification of the intensity of the expression of CB1R (a) , CB2R (b) , GPR55 (c) , PPARα (d) , TRPV1 (e) , TRPA1 (f) , 5-HT1aR (g) , in the suprabasal layers of 7 CTRL- and 8 AD-dogs. Data are represented as Mean ± SD and were analyzed using the Student T -test. P

    Article Snippet: The immunogen of the rabbit anti-TRPV1 (ACC-030) was the peptide (C)EDAEVFK DSMVPGEK (824–838) of rat TRPV1.

    Techniques: Expressing

    Photomicrographs of cryosections of canine skin showing transient receptor potential ankyrin 1 (TRPA1) immunoreactivity (IR) in the tissues of the healthy dogs (CTRL) (a–c) and in the dogs with atopic dermatitis (AD) (d–f) . (a–c) The white arrows indicate some DAPI-labeled nuclei of the keratinocytes of the CTRL dogs showing moderate cytoplasmic TRPV1-IR. The TRPA1 immunolabelling was brighter in the more superficial cells. The open arrows indicate some DAPI-labeled nuclei of the dermal cells showing moderate TRPV1-IR. (d–f) The white arrows indicate some DAPI-labeled nuclei of the keratinocytes of the AD dogs showing bright cytoplasmic TRPV1-IR. Not all the cells of the suprabasal layer showed the same degree of TRPA1-IR which was brighter in the cytoplasm of the more superficial cells. The open arrows indicate some DAPI-labeled nuclei of the dermal cells showing bright TRPA1-IR. Bar, 50 μm.

    Journal: Frontiers in Veterinary Science

    Article Title: Distribution of Cannabinoid Receptors in Keratinocytes of Healthy Dogs and Dogs With Atopic Dermatitis

    doi: 10.3389/fvets.2022.915896

    Figure Lengend Snippet: Photomicrographs of cryosections of canine skin showing transient receptor potential ankyrin 1 (TRPA1) immunoreactivity (IR) in the tissues of the healthy dogs (CTRL) (a–c) and in the dogs with atopic dermatitis (AD) (d–f) . (a–c) The white arrows indicate some DAPI-labeled nuclei of the keratinocytes of the CTRL dogs showing moderate cytoplasmic TRPV1-IR. The TRPA1 immunolabelling was brighter in the more superficial cells. The open arrows indicate some DAPI-labeled nuclei of the dermal cells showing moderate TRPV1-IR. (d–f) The white arrows indicate some DAPI-labeled nuclei of the keratinocytes of the AD dogs showing bright cytoplasmic TRPV1-IR. Not all the cells of the suprabasal layer showed the same degree of TRPA1-IR which was brighter in the cytoplasm of the more superficial cells. The open arrows indicate some DAPI-labeled nuclei of the dermal cells showing bright TRPA1-IR. Bar, 50 μm.

    Article Snippet: The immunogen of the rabbit anti-TRPV1 (ACC-030) was the peptide (C)EDAEVFK DSMVPGEK (824–838) of rat TRPV1.

    Techniques: Labeling