rat il 1β elisa kit  (Boster Bio)

Journal: Molecular Medicine Reports

Article Title: Role of CCR7 on dendritic cell-mediated immune tolerance in the airways of allergy-induced asthmatic rats

doi: 10.3892/mmr.2019.10694

Figure Lengend Snippet: Protein expressions of (A) IFN-γ and IL-4, (B) IL-12 and IL-10, (C) TGF-β and (D) IgE in serum. Protein expression levels of various cytokines were assessed by ELISA. **P

Article Snippet: ELISA kits were used for measuring IFN-γ (cat. no. EK0374), IL-4 (cat. no. EK0406), IL-12 (cat. no. A01152-2), IL-10 (cat. no. EK0418), TGF-β (cat. no. EK0514; all Boster Biological Technology) and IgE (cat. no. E-EL-R0517c; Elabscience Biotechnology, Inc.) concentrations in serum and BALF supernatant, according to the manufacturer's protocol.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay

Journal: Molecular Medicine Reports

Article Title: Role of CCR7 on dendritic cell-mediated immune tolerance in the airways of allergy-induced asthmatic rats

doi: 10.3892/mmr.2019.10694

Figure Lengend Snippet: Protein expression levels of (A) IFN-γ and IL-4, (B) IL-12 and IL-10, (C) TGF-β and (D) IgE in bronchoalveolar lavage fluid. Protein expression levels of various cytokines were assessed by ELISA. **P

Article Snippet: ELISA kits were used for measuring IFN-γ (cat. no. EK0374), IL-4 (cat. no. EK0406), IL-12 (cat. no. A01152-2), IL-10 (cat. no. EK0418), TGF-β (cat. no. EK0514; all Boster Biological Technology) and IgE (cat. no. E-EL-R0517c; Elabscience Biotechnology, Inc.) concentrations in serum and BALF supernatant, according to the manufacturer's protocol.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay

Journal: bioRxiv

Article Title: Immune responses in mice induced by multi-epitope DNA vaccine and protein vaccine of Crimean-Congo Hemorrhagic Fever Virus

doi: 10.1101/719724

Figure Lengend Snippet: ELISA detection of IL-4 and IFN-γ expression levels in mouse spleen cell supernatant After 7 days of the last immunization, the expression of IFN-γ and IL-4 in the supernatant of the mouse spleen T lymphocyte culture was examined. The difference between the treatment groups is statistically significant. **P

Article Snippet: Detection of cytokines IFN-γ and IL-4 levelsThe yields of IL-4 and IFN-γ in T cells were measured by intracellular cytokine staining using the double antibody sandwich method according to the manufacturer instructions of the mice IFN-γ and IL-4 ELISA kits (BOSTER Biological Technology Co. Ltd.Wuhan, China).

Techniques: Enzyme-linked Immunosorbent Assay, Expressing

Journal: International Journal of Nanomedicine

Article Title: Exposure to nickel oxide nanoparticles induces pulmonary inflammation through NLRP3 inflammasome activation in rats

doi: 10.2147/IJN.S106912

Figure Lengend Snippet: NiONPs induce caspase-1-dependent cytokine secretion in RAW264.7 cells. Notes: ( A – C ) The pro-caspase-1 and pro-IL-1β levels in cell lysates (input) and processed caspase-1 and IL-1β levels in culture SNs after treatment with different doses (0 µg/cm 2 , 1 µg/cm 2 , 2 µg/cm 2 , and 4 µg/cm 2 ) of NiONPs were analyzed by Western blotting. ( D ) Caspase-1 activity in RAW264.7 cells. ( E ) Detection of IL-1β expression in the SN by ELISA. * P

Article Snippet: The IL-1β, TNF-α, and IL-18 levels were detected using a mouse IL-1β ELISA Kit (R & D Systems, Inc., Minneapolis, MN, USA), rat IL-1β ELISA Kit (Boster, Wuhan, People’s Republic of China), rat IL-18 ELISA Kit (Boster), and mouse TNF-α ELISA Kit (BioLegend, San Diego, CA, USA), following the manufacturer’s instructions.

Techniques: Western Blot, Activity Assay, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Virology Journal

Article Title: Brain microglia were activated in sporadic CJD but almost unchanged in fatal familial insomnia and G114V genetic CJD

doi: 10.1186/1743-422X-10-216

Figure Lengend Snippet: Analyses of the levels of cytokines in temporal lobes of three FFI cases, a sCJD (Case 1) patient, a G114V gCJD patient and normal control. Cytokines IL-1β, IL-6 and TNF-α in the brain homogenates were individually measured by the respective ELISA kits. The results are calculated from three independent tests and presented as mean ± SD. *: P

Article Snippet: ELISA kits were used to detect the following cytokines: IL-1β, IL-6 and TNF-α (Boster, EK0390; Boster, EK0393; Boster, EK0412; TBD science, KIT0003).

Techniques: Enzyme-linked Immunosorbent Assay