rat brain microvascular endothelial cells  (Cell Applications Inc)

Journal: Scientific Reports

Article Title: Doxycycline prevents blood–brain barrier dysfunction and microvascular hyperpermeability after traumatic brain injury

doi: 10.1038/s41598-022-09394-4

Figure Lengend Snippet: Determination of monolayer permeability using Transwell assays. Doxycycline decreased IL-1β-induced microvascular endothelial cell monolayer hyperpermeability. IL-1β induced hyperpermeability significantly, compared to untreated control group in this model (* p

Article Snippet: The rat brain microvascular endothelial cells (RBMECs) used in some of the experiments were obtained from Cell Applications, Inc. (San Diego, CA).

Techniques: Permeability

Journal: Scientific Reports

Article Title: Doxycycline prevents blood–brain barrier dysfunction and microvascular hyperpermeability after traumatic brain injury

doi: 10.1038/s41598-022-09394-4

Figure Lengend Snippet: ( a ) Intravital microscopic imaging of mouse brain pial venules demonstrating changes in blood–brain barrier/microvascular permeability. Pial venules of 50-75 µm diameter were visualized at 40 × magnification. The imaging was started 10 min after injury or drug administration. Images and video were recorded every 20 min for comparison. Dotted lines have been added to better differentiate the pial venules. ( b ) Graphical plotting of BBB Permeability as Δ I. Two-way ANOVA showed no statistically significant difference among the groups at the initial time point of 10 min after injury. Increased microvascular hyperpermeability at 30, 50, 70 min of TBI was observed in comparison to sham (* p

Article Snippet: The rat brain microvascular endothelial cells (RBMECs) used in some of the experiments were obtained from Cell Applications, Inc. (San Diego, CA).

Techniques: Imaging, Permeability

Journal: Scientific Reports

Article Title: Doxycycline prevents blood–brain barrier dysfunction and microvascular hyperpermeability after traumatic brain injury

doi: 10.1038/s41598-022-09394-4

Figure Lengend Snippet: Evaluation of tight junction integrity by immunofluorescence of ZO-1 in the tight junctions. Rat brain microvascular endothelial cells were exposed to IL-1β and/or pre-treated with doxycycline. ( a ) Localization of ZO-1 at areas of cell-cell contacts/tight junctions. Arrows indicate areas of tight junction disruption. ( b ) Quantification of ZO-1 immunofluorescence. There was significant decrease in the ZO-1 localization in the cells treated with IL-1β (n = 4). This was prevented with treatment of doxycycline (n = 4). The analysis was performed with ImageJ. Compared to control * p

Article Snippet: The rat brain microvascular endothelial cells (RBMECs) used in some of the experiments were obtained from Cell Applications, Inc. (San Diego, CA).

Techniques: Immunofluorescence

Journal: Frontiers in Cell and Developmental Biology

Article Title: Comparison of Anti-oncotic Effect of TRPM4 Blocking Antibody in Neuron, Astrocyte and Vascular Endothelial Cell Under Hypoxia

doi: 10.3389/fcell.2020.562584

Figure Lengend Snippet: M4P blocks TRPM4 activity in cultured rat brain microvascular endothelial cells (RBMVECs) and prevents hypoxia-induced cell swelling. (A) Sample traces from an RBMVEC cell pre-treated with control rabbit IgG (20.8 μg/ml) for 30 min. Acute hypoxia was induced by adding 5 mM NaN 3 and 10 mM 2-DG to the cell for 7 min. 250-ms voltage ramps from -100 to +100 mV was applied at 1 min interval. Holding potential: 0 mV. The pipette solution contained a calculated 7.4 μM free Ca 2+ . (B) Sample traces from an RBMVEC cell pre-treated with M4P at 20.8 μg/ml for 30 min. The recording protocol is similar to that described in (A) . (C) Time-dependent current changes at ±100 mV from (A,B) . All currents were normalized to baseline before hypoxic induction. (D) Summarized current-voltage relationship at 0 min under hypoxia for control IgG ( n = 7) and M4P ( n = 8) treatments. (E) Summary of currents at -100 and 100 mV at 0 min under hypoxia. Control IgG: n = 7; M4P: n = 8. (F) Summarized current-voltage relationship at 7 min under hypoxia for control IgG ( n = 7) and M4P ( n = 8) treatments. (G) Summary of currents at -100 and 100 mV at 7 min under hypoxia. Control IgG: n = 7; M4P: n = 8. (H) Time course of normalized membrane capacitance (C m ) under hypoxic conditions. Control IgG: n = 7; M4P: n = 8. Statistical analysis was performed by two tailed unpaired student’s t- test for (E,G) , and two-way ANOVA test with post hoc Bonferroni’s analysis for (H) . * p

Article Snippet: Rat brain microvascular endothelial cells were purchased from Cell Applications Inc (Cell Applications, San Diego, CA, United States).

Techniques: Activity Assay, Cell Culture, Transferring, Two Tailed Test