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Cell Applications Inc rat brain microvascular endothelial cells rbmecs
Determination of monolayer permeability using Transwell assays. Doxycycline decreased IL-1β-induced <t>microvascular</t> endothelial cell monolayer hyperpermeability. IL-1β induced hyperpermeability significantly, compared to untreated control group in this model (* p
Rat Brain Microvascular Endothelial Cells Rbmecs, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rat brain microvascular endothelial cells rbmecs/product/Cell Applications Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
rat brain microvascular endothelial cells rbmecs - by Bioz Stars, 2022-08
86/100 stars

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1) Product Images from "Doxycycline prevents blood–brain barrier dysfunction and microvascular hyperpermeability after traumatic brain injury"

Article Title: Doxycycline prevents blood–brain barrier dysfunction and microvascular hyperpermeability after traumatic brain injury

Journal: Scientific Reports

doi: 10.1038/s41598-022-09394-4

Determination of monolayer permeability using Transwell assays. Doxycycline decreased IL-1β-induced microvascular endothelial cell monolayer hyperpermeability. IL-1β induced hyperpermeability significantly, compared to untreated control group in this model (* p
Figure Legend Snippet: Determination of monolayer permeability using Transwell assays. Doxycycline decreased IL-1β-induced microvascular endothelial cell monolayer hyperpermeability. IL-1β induced hyperpermeability significantly, compared to untreated control group in this model (* p

Techniques Used: Permeability

( a ) Intravital microscopic imaging of mouse brain pial venules demonstrating changes in blood–brain barrier/microvascular permeability. Pial venules of 50-75 µm diameter were visualized at 40 × magnification. The imaging was started 10 min after injury or drug administration. Images and video were recorded every 20 min for comparison. Dotted lines have been added to better differentiate the pial venules. ( b ) Graphical plotting of BBB Permeability as Δ I. Two-way ANOVA showed no statistically significant difference among the groups at the initial time point of 10 min after injury. Increased microvascular hyperpermeability at 30, 50, 70 min of TBI was observed in comparison to sham (* p
Figure Legend Snippet: ( a ) Intravital microscopic imaging of mouse brain pial venules demonstrating changes in blood–brain barrier/microvascular permeability. Pial venules of 50-75 µm diameter were visualized at 40 × magnification. The imaging was started 10 min after injury or drug administration. Images and video were recorded every 20 min for comparison. Dotted lines have been added to better differentiate the pial venules. ( b ) Graphical plotting of BBB Permeability as Δ I. Two-way ANOVA showed no statistically significant difference among the groups at the initial time point of 10 min after injury. Increased microvascular hyperpermeability at 30, 50, 70 min of TBI was observed in comparison to sham (* p

Techniques Used: Imaging, Permeability

Evaluation of tight junction integrity by immunofluorescence of ZO-1 in the tight junctions. Rat brain microvascular endothelial cells were exposed to IL-1β and/or pre-treated with doxycycline. ( a ) Localization of ZO-1 at areas of cell-cell contacts/tight junctions. Arrows indicate areas of tight junction disruption. ( b ) Quantification of ZO-1 immunofluorescence. There was significant decrease in the ZO-1 localization in the cells treated with IL-1β (n = 4). This was prevented with treatment of doxycycline (n = 4). The analysis was performed with ImageJ. Compared to control * p
Figure Legend Snippet: Evaluation of tight junction integrity by immunofluorescence of ZO-1 in the tight junctions. Rat brain microvascular endothelial cells were exposed to IL-1β and/or pre-treated with doxycycline. ( a ) Localization of ZO-1 at areas of cell-cell contacts/tight junctions. Arrows indicate areas of tight junction disruption. ( b ) Quantification of ZO-1 immunofluorescence. There was significant decrease in the ZO-1 localization in the cells treated with IL-1β (n = 4). This was prevented with treatment of doxycycline (n = 4). The analysis was performed with ImageJ. Compared to control * p

Techniques Used: Immunofluorescence

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    Cell Applications Inc rat brain microvascular endothelial cells rbmecs
    Determination of monolayer permeability using Transwell assays. Doxycycline decreased IL-1β-induced <t>microvascular</t> endothelial cell monolayer hyperpermeability. IL-1β induced hyperpermeability significantly, compared to untreated control group in this model (* p
    Rat Brain Microvascular Endothelial Cells Rbmecs, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rat brain microvascular endothelial cells rbmecs/product/Cell Applications Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rat brain microvascular endothelial cells rbmecs - by Bioz Stars, 2022-08
    86/100 stars
      Buy from Supplier

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    Determination of monolayer permeability using Transwell assays. Doxycycline decreased IL-1β-induced microvascular endothelial cell monolayer hyperpermeability. IL-1β induced hyperpermeability significantly, compared to untreated control group in this model (* p

    Journal: Scientific Reports

    Article Title: Doxycycline prevents blood–brain barrier dysfunction and microvascular hyperpermeability after traumatic brain injury

    doi: 10.1038/s41598-022-09394-4

    Figure Lengend Snippet: Determination of monolayer permeability using Transwell assays. Doxycycline decreased IL-1β-induced microvascular endothelial cell monolayer hyperpermeability. IL-1β induced hyperpermeability significantly, compared to untreated control group in this model (* p

    Article Snippet: The rat brain microvascular endothelial cells (RBMECs) used in some of the experiments were obtained from Cell Applications, Inc. (San Diego, CA).

    Techniques: Permeability

    ( a ) Intravital microscopic imaging of mouse brain pial venules demonstrating changes in blood–brain barrier/microvascular permeability. Pial venules of 50-75 µm diameter were visualized at 40 × magnification. The imaging was started 10 min after injury or drug administration. Images and video were recorded every 20 min for comparison. Dotted lines have been added to better differentiate the pial venules. ( b ) Graphical plotting of BBB Permeability as Δ I. Two-way ANOVA showed no statistically significant difference among the groups at the initial time point of 10 min after injury. Increased microvascular hyperpermeability at 30, 50, 70 min of TBI was observed in comparison to sham (* p

    Journal: Scientific Reports

    Article Title: Doxycycline prevents blood–brain barrier dysfunction and microvascular hyperpermeability after traumatic brain injury

    doi: 10.1038/s41598-022-09394-4

    Figure Lengend Snippet: ( a ) Intravital microscopic imaging of mouse brain pial venules demonstrating changes in blood–brain barrier/microvascular permeability. Pial venules of 50-75 µm diameter were visualized at 40 × magnification. The imaging was started 10 min after injury or drug administration. Images and video were recorded every 20 min for comparison. Dotted lines have been added to better differentiate the pial venules. ( b ) Graphical plotting of BBB Permeability as Δ I. Two-way ANOVA showed no statistically significant difference among the groups at the initial time point of 10 min after injury. Increased microvascular hyperpermeability at 30, 50, 70 min of TBI was observed in comparison to sham (* p

    Article Snippet: The rat brain microvascular endothelial cells (RBMECs) used in some of the experiments were obtained from Cell Applications, Inc. (San Diego, CA).

    Techniques: Imaging, Permeability

    Evaluation of tight junction integrity by immunofluorescence of ZO-1 in the tight junctions. Rat brain microvascular endothelial cells were exposed to IL-1β and/or pre-treated with doxycycline. ( a ) Localization of ZO-1 at areas of cell-cell contacts/tight junctions. Arrows indicate areas of tight junction disruption. ( b ) Quantification of ZO-1 immunofluorescence. There was significant decrease in the ZO-1 localization in the cells treated with IL-1β (n = 4). This was prevented with treatment of doxycycline (n = 4). The analysis was performed with ImageJ. Compared to control * p

    Journal: Scientific Reports

    Article Title: Doxycycline prevents blood–brain barrier dysfunction and microvascular hyperpermeability after traumatic brain injury

    doi: 10.1038/s41598-022-09394-4

    Figure Lengend Snippet: Evaluation of tight junction integrity by immunofluorescence of ZO-1 in the tight junctions. Rat brain microvascular endothelial cells were exposed to IL-1β and/or pre-treated with doxycycline. ( a ) Localization of ZO-1 at areas of cell-cell contacts/tight junctions. Arrows indicate areas of tight junction disruption. ( b ) Quantification of ZO-1 immunofluorescence. There was significant decrease in the ZO-1 localization in the cells treated with IL-1β (n = 4). This was prevented with treatment of doxycycline (n = 4). The analysis was performed with ImageJ. Compared to control * p

    Article Snippet: The rat brain microvascular endothelial cells (RBMECs) used in some of the experiments were obtained from Cell Applications, Inc. (San Diego, CA).

    Techniques: Immunofluorescence