rat brain microvascular endothelial cells rbmecs  (Cell Applications Inc)

Journal: Scientific Reports

Article Title: Doxycycline prevents blood–brain barrier dysfunction and microvascular hyperpermeability after traumatic brain injury

doi: 10.1038/s41598-022-09394-4

Figure Lengend Snippet: Determination of monolayer permeability using Transwell assays. Doxycycline decreased IL-1β-induced microvascular endothelial cell monolayer hyperpermeability. IL-1β induced hyperpermeability significantly, compared to untreated control group in this model (* p

Article Snippet: The rat brain microvascular endothelial cells (RBMECs) used in some of the experiments were obtained from Cell Applications, Inc. (San Diego, CA).

Techniques: Permeability

Journal: Scientific Reports

Article Title: Doxycycline prevents blood–brain barrier dysfunction and microvascular hyperpermeability after traumatic brain injury

doi: 10.1038/s41598-022-09394-4

Figure Lengend Snippet: ( a ) Intravital microscopic imaging of mouse brain pial venules demonstrating changes in blood–brain barrier/microvascular permeability. Pial venules of 50-75 µm diameter were visualized at 40 × magnification. The imaging was started 10 min after injury or drug administration. Images and video were recorded every 20 min for comparison. Dotted lines have been added to better differentiate the pial venules. ( b ) Graphical plotting of BBB Permeability as Δ I. Two-way ANOVA showed no statistically significant difference among the groups at the initial time point of 10 min after injury. Increased microvascular hyperpermeability at 30, 50, 70 min of TBI was observed in comparison to sham (* p

Article Snippet: The rat brain microvascular endothelial cells (RBMECs) used in some of the experiments were obtained from Cell Applications, Inc. (San Diego, CA).

Techniques: Imaging, Permeability

Journal: Scientific Reports

Article Title: Doxycycline prevents blood–brain barrier dysfunction and microvascular hyperpermeability after traumatic brain injury

doi: 10.1038/s41598-022-09394-4

Figure Lengend Snippet: Evaluation of tight junction integrity by immunofluorescence of ZO-1 in the tight junctions. Rat brain microvascular endothelial cells were exposed to IL-1β and/or pre-treated with doxycycline. ( a ) Localization of ZO-1 at areas of cell-cell contacts/tight junctions. Arrows indicate areas of tight junction disruption. ( b ) Quantification of ZO-1 immunofluorescence. There was significant decrease in the ZO-1 localization in the cells treated with IL-1β (n = 4). This was prevented with treatment of doxycycline (n = 4). The analysis was performed with ImageJ. Compared to control * p

Article Snippet: The rat brain microvascular endothelial cells (RBMECs) used in some of the experiments were obtained from Cell Applications, Inc. (San Diego, CA).

Techniques: Immunofluorescence