beta III Tubulin Overexpression Lysate Denatured
0 1 ml
https://www.bioz.com/result/rat anti tubulin/product/Novus Biologicals
Average 91 stars, based on 1 article reviews
Price from $9.99 to $1999.99
1) Product Images from "Ipl1/Aurora Kinase Suppresses S-CDK-Driven Spindle Formation during Prophase I to Ensure Chromosome Integrity during Meiosis"
Article Title: Ipl1/Aurora Kinase Suppresses S-CDK-Driven Spindle Formation during Prophase I to Ensure Chromosome Integrity during Meiosis
Journal: PLoS ONE
Figure Legend Snippet: Ipl1 prevents formation of spindles in DDR-arrested cells. (A) Proportion of cells with separated spindle-pole bodies as a function of time. Strains: Wild type (Y940), ipl1-md (Y1206), dmc1 Δ (Y2266), ipl1-md dmc1 Δ (Y2268), hop2 Δ (Y2489), hop2 Δ ip1-mn (Y2491) rec8 Δ (Y2404), rec8 Δ ipl1-md (Y2457). Three independent diploids were assessed, a representative time course is shown for each strain. (B, C) Tubulin configurations observed in dmc1 Δ ipl1-md mutants and their prevalence (C). (D) Representative examples of spindle configurations from a single frame (maximum intensity projection) from time lapse imaging in dmc1 Δ and dmc1 Δ ipl1-md mutants. (E) The cumulative proportion of cells that formed spindles during the three hours of time-lapse imaging (8–11 h). (F) Representative example dynamic behaviour of tubulin (Tub1-GFP) and DNA (H2B-mCherry) during time-lapse imaging of the dmc1 Δ ipl1-md mutant. (G) Western blot showing that Ipl1 is efficiently depleted in dmc1 Δ ipl1-md cells.
Techniques Used: Imaging, Mutagenesis, Western Blot
Figure Legend Snippet: S-CDK is required and sufficient to drive SPB separation and spindle formation during prophase I in ipl1-md cells. (A)Images for tubulin and Zip1 staining in dmc1 Δ ipl1-md strains with normal S-CDK and M-CDK (left image), lacking S-CDK activity ( clb5 Δ clb6 Δ; middle image), or without M-CDK proficient for Clb5 only ( clb1 Δ, clb3 Δ, clb4 Δ, clb6 Δ CLB5 + ; right panel). Strains: Y4495, Y4435, and Y4496, respectively. Bars, 2 µm. (B) Quantification on the proportion of fixed cells with spindles and separated SPBs at 8 hours and 12 hours. (C, D) ipl1-md ndt80 Δ cdc28-as1 (Y2577) cells were treated with either 50 µM 1-NM-PP1 (+) or solvent only (DMSO) (−) to inhibit Cdc28/CDK kinase activity at 6 hours, when spindles have formed in at least 20% of ipl1-md ndt80 Δ cells. Examples of spread, meiotic nuclei are shown to the left. Note that there was no effect on inhibiting Cdc28-as1 in ndt80 Δ alone bars, 2 µm. The graph shows that Quantification of prophase spreads with spindles or aberrant spindle pole structures ( e.g. multipolar spindles).
Techniques Used: Staining, Activity Assay
Figure Legend Snippet: Ipl1 depletion causes precocious formation of spindles in prophase I-arrested ndt80 mutants. (A) Representative examples of SPB and spindle configurations in ndt80 Δ and ndt80 Δ ipl1-md mutants. (B) The proportion of cells that formed spindles during the four hours of time-lapse imaging. A small number multipolar spindles were observed; these were added to the ‘spindle’ category. (C) Representative example dynamic behaviour of tubulin during time-lapse imaging of the ndt80 Δ ipl1-md mutant. (D) Spindle formation in ipl1-md cells arrested in prophase I (t = 0; 6 hours in sporulation medium), and after release using the ndt80-IN system (WT: Y967 and ipl1-mn :Y1169). The spindle and SPB conformation were assessed in > 100 cells every 15 min. after release from NDT80 arrest.
Techniques Used: Imaging, Mutagenesis
Article Title: Recruitment of vimentin to the cell surface by ?3 integrin and plectin mediates adhesion strength
Article Snippet: Antibodies used were mouse anti-αvβ3 integrin (LM609), rabbit anti-β3 integrin, mouse anti-αv integrin (Chemicon, Temecula, CA), mouse anti-β5 integrin (Calbiochem, San Diego, CA), goat anti-β3 integrin (N-20) (Santa Cruz Biotechnology, Santa Cruz, CA), rabbit anti-FAK [pY397] (Invitrogen, Carlsbad, CA), rabbit monoclonal anti-paxillin (Epitomics, Burlingame, CA), rabbit monoclonal anti-plectin (Epitomics), rabbit anti-dynein heavy chain (Santa Cruz Biotechnology), mouse anti-kinesin heavy chain (Chemicon), rabbit monoclonal anti-actin (Epitomics),
Article Title: α-Synuclein in gut endocrine cells and its implications for Parkinson’s disease
Article Snippet: .. When cells had reached the desired confluency, they were washed with PBS and fixed for 10 minutes in 10% formalin. β-Tubulin polyclonal antibody raised in
Article Title: Loss of hepatic aryl hydrocarbon receptor protein in adrenalectomized rats does not involve altered levels of the receptor's cytoplasmic chaperones
Article Snippet: .. Blots derived from gels originally loaded with 20 or 25 μg of hepatic cytosolic protein were probed with the following primary antibodies: mouse monoclonal against human AIP, dilution 1:10,000 (Dr. Christopher A. Bradfield, McArdle Laboratory for Cancer Research, Madison, WI); mouse monoclonal against a human hsp90β peptide, dilution 1:50,000 (Santa Cruz Biotechnology, Santa Cruz, CA); mouse monoclonal against a human p23 peptide, dilution 1:2,000 (Santa Cruz Biotechnology); and mouse monoclonal against
Article Title: Metabolic Maintenance of Cell Asymmetry following Division in Activated T Lymphocytes
Article Snippet: .. T Cell Imaging Antibodies against the following antigens were used: CD8 (13-0081-85; eBioscience), β-tubulin (T8328; Sigma-Aldrich), Numb (ab14140; Abcam), Scribble (ab154067; Abcam),