rapid dna ligation kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher rapid dna ligation kit
    Generation of pUbC-FLuc-ROSA αPIG-iPSC. A. Genomic <t>DNA</t> was isolated from indicated cell lines, digested with Eco RI and hybridized with a probe for ROSA26 locus in a Southern blot analysis. Expected size of the wild type allele is 15630 bp and of successfully targeted allele 4066 bp because the integration of FLuc cassette results in an additional Eco RI restriction site. Presence of both bands in transgenic clones indicates heterozygous integration. B. <t>PCR</t> amplification of genomic DNA isolated from indicated cell lines and donor plasmid to verify the site specific integration of FLuc-expression cassette into the ROSA26 locus with an expected PCR product size of 950 kb. C. Representative brightfield and fluorescent images of puromycin-purified day 16 pUbC-FLuc-ROSA αPIG-iPSC-derived EGFP positive cardiac clusters. Scale bar = 500 µm. D. Relative luminescence units (RLU) per µg protein in the two clones (C3 and C5) of undifferentiated pUbC-FLuc-ROSA αPIG-iPSC and CM derived from them on day 16 of differentiation. Values are from triplicate measurements of three independent differentiations. Statistics: two-tailed paired Student's t-test. **p
    Rapid Dna Ligation Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rapid dna ligation kit/product/Thermo Fisher
    Average 99 stars, based on 76 article reviews
    Price from $9.99 to $1999.99
    rapid dna ligation kit - by Bioz Stars, 2019-12
    99/100 stars

    Images

    1) Product Images from "Bioluminescent Imaging of Genetically Selected Induced Pluripotent Stem Cell-Derived Cardiomyocytes after Transplantation into Infarcted Heart of Syngeneic Recipients"

    Article Title: Bioluminescent Imaging of Genetically Selected Induced Pluripotent Stem Cell-Derived Cardiomyocytes after Transplantation into Infarcted Heart of Syngeneic Recipients

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0107363

    Generation of pUbC-FLuc-ROSA αPIG-iPSC. A. Genomic DNA was isolated from indicated cell lines, digested with Eco RI and hybridized with a probe for ROSA26 locus in a Southern blot analysis. Expected size of the wild type allele is 15630 bp and of successfully targeted allele 4066 bp because the integration of FLuc cassette results in an additional Eco RI restriction site. Presence of both bands in transgenic clones indicates heterozygous integration. B. PCR amplification of genomic DNA isolated from indicated cell lines and donor plasmid to verify the site specific integration of FLuc-expression cassette into the ROSA26 locus with an expected PCR product size of 950 kb. C. Representative brightfield and fluorescent images of puromycin-purified day 16 pUbC-FLuc-ROSA αPIG-iPSC-derived EGFP positive cardiac clusters. Scale bar = 500 µm. D. Relative luminescence units (RLU) per µg protein in the two clones (C3 and C5) of undifferentiated pUbC-FLuc-ROSA αPIG-iPSC and CM derived from them on day 16 of differentiation. Values are from triplicate measurements of three independent differentiations. Statistics: two-tailed paired Student's t-test. **p
    Figure Legend Snippet: Generation of pUbC-FLuc-ROSA αPIG-iPSC. A. Genomic DNA was isolated from indicated cell lines, digested with Eco RI and hybridized with a probe for ROSA26 locus in a Southern blot analysis. Expected size of the wild type allele is 15630 bp and of successfully targeted allele 4066 bp because the integration of FLuc cassette results in an additional Eco RI restriction site. Presence of both bands in transgenic clones indicates heterozygous integration. B. PCR amplification of genomic DNA isolated from indicated cell lines and donor plasmid to verify the site specific integration of FLuc-expression cassette into the ROSA26 locus with an expected PCR product size of 950 kb. C. Representative brightfield and fluorescent images of puromycin-purified day 16 pUbC-FLuc-ROSA αPIG-iPSC-derived EGFP positive cardiac clusters. Scale bar = 500 µm. D. Relative luminescence units (RLU) per µg protein in the two clones (C3 and C5) of undifferentiated pUbC-FLuc-ROSA αPIG-iPSC and CM derived from them on day 16 of differentiation. Values are from triplicate measurements of three independent differentiations. Statistics: two-tailed paired Student's t-test. **p

    Techniques Used: Isolation, Southern Blot, Transgenic Assay, Clone Assay, Polymerase Chain Reaction, Amplification, Plasmid Preparation, Expressing, Purification, Derivative Assay, Two Tailed Test

    2) Product Images from "Generating aldehyde-tagged antibodies with high titers and high formylglycine yields by supplementing culture media with copper(II)"

    Article Title: Generating aldehyde-tagged antibodies with high titers and high formylglycine yields by supplementing culture media with copper(II)

    Journal: BMC Biotechnology

    doi: 10.1186/s12896-016-0254-0

    High fGly conversion yields can be obtained from stably transduced cell lines under high titer cell culture conditions. Stable transduction of DNA encoding FGE, antibody light chain, and antibody heavy chain bearing one (CT only) or two (C H 1 and CT) aldehyde tags was performed with viral retrovector transduction into CHO cells (GPEx® technology). The resulting stable pools were cultured in three types of media +/- supplementation with copper(II) sulfate ( n = 3). Titers ( a ) and fGly conversion ( b ) of CT-tagged antibodies were assessed. Then, the stable pools were cloned by limiting dilution and clone performance was assessed in fed batch cultures ( c and d ; shake flask, n = 5 clones; bioreactor, n = 3 clones single tag, n = 1 clone double tag). The top performing stable single-tagged clonal cell line produced antibody in very high titer (5.2 g/L) with 98 % conversion of Cys to fGly ( c and d ). The specific productivity (75 pg/cell/d) of this top clone demonstrates the capabilities of the GPEx® technology for efficient production of highly converted antibody. The generation of fGly in single- and doubly-tagged antibodies scaled successfully to bioreactors (2 L cultures; c and d ). Error bars indicate standard deviation
    Figure Legend Snippet: High fGly conversion yields can be obtained from stably transduced cell lines under high titer cell culture conditions. Stable transduction of DNA encoding FGE, antibody light chain, and antibody heavy chain bearing one (CT only) or two (C H 1 and CT) aldehyde tags was performed with viral retrovector transduction into CHO cells (GPEx® technology). The resulting stable pools were cultured in three types of media +/- supplementation with copper(II) sulfate ( n = 3). Titers ( a ) and fGly conversion ( b ) of CT-tagged antibodies were assessed. Then, the stable pools were cloned by limiting dilution and clone performance was assessed in fed batch cultures ( c and d ; shake flask, n = 5 clones; bioreactor, n = 3 clones single tag, n = 1 clone double tag). The top performing stable single-tagged clonal cell line produced antibody in very high titer (5.2 g/L) with 98 % conversion of Cys to fGly ( c and d ). The specific productivity (75 pg/cell/d) of this top clone demonstrates the capabilities of the GPEx® technology for efficient production of highly converted antibody. The generation of fGly in single- and doubly-tagged antibodies scaled successfully to bioreactors (2 L cultures; c and d ). Error bars indicate standard deviation

    Techniques Used: Stable Transfection, Cell Culture, Transduction, Clone Assay, Produced, Standard Deviation

    3) Product Images from "A combination of transposable elements and magnetic cell sorting provides a very efficient transgenesis system for chicken primary erythroid progenitors"

    Article Title: A combination of transposable elements and magnetic cell sorting provides a very efficient transgenesis system for chicken primary erythroid progenitors

    Journal: BMC Biotechnology

    doi: 10.1186/1472-6750-9-81

    Identification of the Tol2 construct genomic insertion sites on the T2EC clone #1 . A : Splinkerette PCR products on 1.5% agar gel. 1: Fermentas DNA Ladder Mix; 2: genomic DNA from non-transfected T2EC; 3: pT2.CMV-hKO plasmid; 4: genomic DNA from T2EC clone #1, cotransfected with the pT2.CMV-hKO plasmid and the transposase plasmid, pCAGGS-T2TP (molecular ratio: 5/1). B : Alignment of the splinkerette PCR product with the Gallus gallus genome. The sequence corresponding to the Tol2 construct is shown in bold . C : Localization of the Tol2 construct insertion site into chromosome 4 of the Gallus gallus genome.
    Figure Legend Snippet: Identification of the Tol2 construct genomic insertion sites on the T2EC clone #1 . A : Splinkerette PCR products on 1.5% agar gel. 1: Fermentas DNA Ladder Mix; 2: genomic DNA from non-transfected T2EC; 3: pT2.CMV-hKO plasmid; 4: genomic DNA from T2EC clone #1, cotransfected with the pT2.CMV-hKO plasmid and the transposase plasmid, pCAGGS-T2TP (molecular ratio: 5/1). B : Alignment of the splinkerette PCR product with the Gallus gallus genome. The sequence corresponding to the Tol2 construct is shown in bold . C : Localization of the Tol2 construct insertion site into chromosome 4 of the Gallus gallus genome.

    Techniques Used: Construct, Polymerase Chain Reaction, Transfection, Plasmid Preparation, Sequencing

    4) Product Images from "FlgM as a Secretion Moiety for the Development of an Inducible Type III Secretion System"

    Article Title: FlgM as a Secretion Moiety for the Development of an Inducible Type III Secretion System

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0059034

    Transcription of the master operon flhDC . ( A ) To verify the transcription of the plasmid-encoded master operon flhDC Reverse Transcriptase PCR Reaction of the isolated RNA was performed. It was assumed that within the overnight culture cells exhibiting flagellar assembly were found and therefore this culture was used as a positive control. Cells before addition of the inducer IPTG were used as reference compared to cells after induction of the plasmid-encoded flhDC . To evaluate genomic DNA contamination the isolated RNA samples were also subjected to subsequent PCR. Plasmid-encoded flhDC was used as a positive control for the PCR reaction. Fermentas 1 KB GeneRuler, ON overnight culture sample, -I whole cell sample without induction of plasmid-encoded flhDC , +I whole cell samples with induction of plasmid-encoded flhDC , RT Reverse Transcriptase Reaction, + plasmid-encoded flhDC ; ( B ) Scheme of the plasmid-encoded master operon under the control of the lacUV5 promoter
    Figure Legend Snippet: Transcription of the master operon flhDC . ( A ) To verify the transcription of the plasmid-encoded master operon flhDC Reverse Transcriptase PCR Reaction of the isolated RNA was performed. It was assumed that within the overnight culture cells exhibiting flagellar assembly were found and therefore this culture was used as a positive control. Cells before addition of the inducer IPTG were used as reference compared to cells after induction of the plasmid-encoded flhDC . To evaluate genomic DNA contamination the isolated RNA samples were also subjected to subsequent PCR. Plasmid-encoded flhDC was used as a positive control for the PCR reaction. Fermentas 1 KB GeneRuler, ON overnight culture sample, -I whole cell sample without induction of plasmid-encoded flhDC , +I whole cell samples with induction of plasmid-encoded flhDC , RT Reverse Transcriptase Reaction, + plasmid-encoded flhDC ; ( B ) Scheme of the plasmid-encoded master operon under the control of the lacUV5 promoter

    Techniques Used: Plasmid Preparation, Polymerase Chain Reaction, Isolation, Positive Control

    Related Articles

    Clone Assay:

    Article Title: A FRET-Based DNA Biosensor Tracks OmpR-Dependent Acidification of Salmonella during Macrophage InfectionHow Salmonella Survives the Macrophage's Acid Attack
    Article Snippet: The PCR products and low copy promoter-less pWSK29 vector were double-digested with restriction enzymes XhoI and BamHI or KpnI and HindIII (Thermo Fisher Scientific), and the digested samples were ligated with the Rapid DNA Ligation Kit (Thermo Fisher Scientific). .. The PCR products and low copy promoter-less pWSK29 vector were double-digested with restriction enzymes XhoI and BamHI or KpnI and HindIII (Thermo Fisher Scientific), and the digested samples were ligated with the Rapid DNA Ligation Kit (Thermo Fisher Scientific).

    Amplification:

    Article Title: A FRET-Based DNA Biosensor Tracks OmpR-Dependent Acidification of Salmonella during Macrophage InfectionHow Salmonella Survives the Macrophage's Acid Attack
    Article Snippet: The sseJ -HA fusion or ompR DNA sequences were amplified using primers XhoI-PsseJ #F and sseJ-HA-BamHI #R or KpnI-PompR #F and envZ- HindIII #R from Salmonella 14028s genomic DNA. .. The PCR products and low copy promoter-less pWSK29 vector were double-digested with restriction enzymes XhoI and BamHI or KpnI and HindIII (Thermo Fisher Scientific), and the digested samples were ligated with the Rapid DNA Ligation Kit (Thermo Fisher Scientific).

    DNA Ligation:

    Article Title: A FRET-Based DNA Biosensor Tracks OmpR-Dependent Acidification of Salmonella during Macrophage InfectionHow Salmonella Survives the Macrophage's Acid Attack
    Article Snippet: The sseJ -HA fusion or ompR DNA sequences were amplified using primers XhoI-PsseJ #F and sseJ-HA-BamHI #R or KpnI-PompR #F and envZ- HindIII #R from Salmonella 14028s genomic DNA. .. The PCR products and low copy promoter-less pWSK29 vector were double-digested with restriction enzymes XhoI and BamHI or KpnI and HindIII (Thermo Fisher Scientific), and the digested samples were ligated with the Rapid DNA Ligation Kit (Thermo Fisher Scientific). .. The cadA , cadB , cadC , ompC , ompF , mgtC , atpB , and sseB genes were deleted from the Salmonella chromosome using λ-Red recombination techniques as described previously [ , ].

    Article Title: Protein kinase A-dependent phosphorylation stimulates the transcriptional activity of hypoxia-inducible factor 1
    Article Snippet: Missense mutants of HIF-1α were generated by PCR using the Q5 Site-Directed Mutagenesis Kit and primers ( ) designed by NEBaseChanger software (New England Biolabs). .. His6 -R1a vector was generated by isolation of bovine R1a cDNA sequences from pRSETB (Addgene, plasmid 14922) as a HindIII/NdeI fragment and ligation to pET15b (Rapid DNA Ligation Kit, Thermo Scientific). .. Other plasmids have been previously described ( ).

    Ligation:

    Article Title: Protein kinase A-dependent phosphorylation stimulates the transcriptional activity of hypoxia-inducible factor 1
    Article Snippet: Missense mutants of HIF-1α were generated by PCR using the Q5 Site-Directed Mutagenesis Kit and primers ( ) designed by NEBaseChanger software (New England Biolabs). .. His6 -R1a vector was generated by isolation of bovine R1a cDNA sequences from pRSETB (Addgene, plasmid 14922) as a HindIII/NdeI fragment and ligation to pET15b (Rapid DNA Ligation Kit, Thermo Scientific). .. Other plasmids have been previously described ( ).

    Mutagenesis:

    Article Title: Protein kinase A-dependent phosphorylation stimulates the transcriptional activity of hypoxia-inducible factor 1
    Article Snippet: Missense mutants of HIF-1α were generated by PCR using the Q5 Site-Directed Mutagenesis Kit and primers ( ) designed by NEBaseChanger software (New England Biolabs). .. His6 -R1a vector was generated by isolation of bovine R1a cDNA sequences from pRSETB (Addgene, plasmid 14922) as a HindIII/NdeI fragment and ligation to pET15b (Rapid DNA Ligation Kit, Thermo Scientific).

    Isolation:

    Article Title: Protein kinase A-dependent phosphorylation stimulates the transcriptional activity of hypoxia-inducible factor 1
    Article Snippet: Missense mutants of HIF-1α were generated by PCR using the Q5 Site-Directed Mutagenesis Kit and primers ( ) designed by NEBaseChanger software (New England Biolabs). .. His6 -R1a vector was generated by isolation of bovine R1a cDNA sequences from pRSETB (Addgene, plasmid 14922) as a HindIII/NdeI fragment and ligation to pET15b (Rapid DNA Ligation Kit, Thermo Scientific). .. Other plasmids have been previously described ( ).

    Construct:

    Article Title: A FRET-Based DNA Biosensor Tracks OmpR-Dependent Acidification of Salmonella during Macrophage InfectionHow Salmonella Survives the Macrophage's Acid Attack
    Article Snippet: The PCR products and low copy promoter-less pWSK29 vector were double-digested with restriction enzymes XhoI and BamHI or KpnI and HindIII (Thermo Fisher Scientific), and the digested samples were ligated with the Rapid DNA Ligation Kit (Thermo Fisher Scientific). .. A cadBA over-expressed strain was generated by cloning cadBA into plasmid pBR322, replacing the bla gene with cadBA .

    Article Title: Protein kinase A-dependent phosphorylation stimulates the transcriptional activity of hypoxia-inducible factor 1
    Article Snippet: Paragraph title: Plasmid constructs and recombinant protein expression ... His6 -R1a vector was generated by isolation of bovine R1a cDNA sequences from pRSETB (Addgene, plasmid 14922) as a HindIII/NdeI fragment and ligation to pET15b (Rapid DNA Ligation Kit, Thermo Scientific).

    Purification:

    Article Title: Protein kinase A-dependent phosphorylation stimulates the transcriptional activity of hypoxia-inducible factor 1
    Article Snippet: His6 -R1a vector was generated by isolation of bovine R1a cDNA sequences from pRSETB (Addgene, plasmid 14922) as a HindIII/NdeI fragment and ligation to pET15b (Rapid DNA Ligation Kit, Thermo Scientific). .. His6 -R1a vector was generated by isolation of bovine R1a cDNA sequences from pRSETB (Addgene, plasmid 14922) as a HindIII/NdeI fragment and ligation to pET15b (Rapid DNA Ligation Kit, Thermo Scientific).

    Generated:

    Article Title: A FRET-Based DNA Biosensor Tracks OmpR-Dependent Acidification of Salmonella during Macrophage InfectionHow Salmonella Survives the Macrophage's Acid Attack
    Article Snippet: The PCR products and low copy promoter-less pWSK29 vector were double-digested with restriction enzymes XhoI and BamHI or KpnI and HindIII (Thermo Fisher Scientific), and the digested samples were ligated with the Rapid DNA Ligation Kit (Thermo Fisher Scientific). .. The PCR products and low copy promoter-less pWSK29 vector were double-digested with restriction enzymes XhoI and BamHI or KpnI and HindIII (Thermo Fisher Scientific), and the digested samples were ligated with the Rapid DNA Ligation Kit (Thermo Fisher Scientific).

    Article Title: Protein kinase A-dependent phosphorylation stimulates the transcriptional activity of hypoxia-inducible factor 1
    Article Snippet: Missense mutants of HIF-1α were generated by PCR using the Q5 Site-Directed Mutagenesis Kit and primers ( ) designed by NEBaseChanger software (New England Biolabs). .. His6 -R1a vector was generated by isolation of bovine R1a cDNA sequences from pRSETB (Addgene, plasmid 14922) as a HindIII/NdeI fragment and ligation to pET15b (Rapid DNA Ligation Kit, Thermo Scientific). .. Other plasmids have been previously described ( ).

    shRNA:

    Article Title: Protein kinase A-dependent phosphorylation stimulates the transcriptional activity of hypoxia-inducible factor 1
    Article Snippet: Five shRNA vectors targeting Ca (MISSION shRNA, Sigma-Aldrich) were screened for knockdown efficiency and vectors encoding shCa-1 (TRCN0000233525; ) and shCa-5 (TRCN0000356094; ) were used to generate stable puromycin-resistant HeLa subclones. .. His6 -R1a vector was generated by isolation of bovine R1a cDNA sequences from pRSETB (Addgene, plasmid 14922) as a HindIII/NdeI fragment and ligation to pET15b (Rapid DNA Ligation Kit, Thermo Scientific).

    Expressing:

    Article Title: Protein kinase A-dependent phosphorylation stimulates the transcriptional activity of hypoxia-inducible factor 1
    Article Snippet: Paragraph title: Plasmid constructs and recombinant protein expression ... His6 -R1a vector was generated by isolation of bovine R1a cDNA sequences from pRSETB (Addgene, plasmid 14922) as a HindIII/NdeI fragment and ligation to pET15b (Rapid DNA Ligation Kit, Thermo Scientific).

    Polymerase Chain Reaction:

    Article Title: A FRET-Based DNA Biosensor Tracks OmpR-Dependent Acidification of Salmonella during Macrophage InfectionHow Salmonella Survives the Macrophage's Acid Attack
    Article Snippet: The sseJ -HA fusion or ompR DNA sequences were amplified using primers XhoI-PsseJ #F and sseJ-HA-BamHI #R or KpnI-PompR #F and envZ- HindIII #R from Salmonella 14028s genomic DNA. .. The PCR products and low copy promoter-less pWSK29 vector were double-digested with restriction enzymes XhoI and BamHI or KpnI and HindIII (Thermo Fisher Scientific), and the digested samples were ligated with the Rapid DNA Ligation Kit (Thermo Fisher Scientific). .. The cadA , cadB , cadC , ompC , ompF , mgtC , atpB , and sseB genes were deleted from the Salmonella chromosome using λ-Red recombination techniques as described previously [ , ].

    Article Title: Protein kinase A-dependent phosphorylation stimulates the transcriptional activity of hypoxia-inducible factor 1
    Article Snippet: Missense mutants of HIF-1α were generated by PCR using the Q5 Site-Directed Mutagenesis Kit and primers ( ) designed by NEBaseChanger software (New England Biolabs). .. His6 -R1a vector was generated by isolation of bovine R1a cDNA sequences from pRSETB (Addgene, plasmid 14922) as a HindIII/NdeI fragment and ligation to pET15b (Rapid DNA Ligation Kit, Thermo Scientific).

    Recombinant:

    Article Title: Protein kinase A-dependent phosphorylation stimulates the transcriptional activity of hypoxia-inducible factor 1
    Article Snippet: Paragraph title: Plasmid constructs and recombinant protein expression ... His6 -R1a vector was generated by isolation of bovine R1a cDNA sequences from pRSETB (Addgene, plasmid 14922) as a HindIII/NdeI fragment and ligation to pET15b (Rapid DNA Ligation Kit, Thermo Scientific).

    Hemagglutination Assay:

    Article Title: A FRET-Based DNA Biosensor Tracks OmpR-Dependent Acidification of Salmonella during Macrophage InfectionHow Salmonella Survives the Macrophage's Acid Attack
    Article Snippet: Paragraph title: Construction of Mutants and HA/GFP/mCherry Tagged Strains ... The PCR products and low copy promoter-less pWSK29 vector were double-digested with restriction enzymes XhoI and BamHI or KpnI and HindIII (Thermo Fisher Scientific), and the digested samples were ligated with the Rapid DNA Ligation Kit (Thermo Fisher Scientific).

    Plasmid Preparation:

    Article Title: A FRET-Based DNA Biosensor Tracks OmpR-Dependent Acidification of Salmonella during Macrophage InfectionHow Salmonella Survives the Macrophage's Acid Attack
    Article Snippet: The sseJ -HA fusion or ompR DNA sequences were amplified using primers XhoI-PsseJ #F and sseJ-HA-BamHI #R or KpnI-PompR #F and envZ- HindIII #R from Salmonella 14028s genomic DNA. .. The PCR products and low copy promoter-less pWSK29 vector were double-digested with restriction enzymes XhoI and BamHI or KpnI and HindIII (Thermo Fisher Scientific), and the digested samples were ligated with the Rapid DNA Ligation Kit (Thermo Fisher Scientific). .. The cadA , cadB , cadC , ompC , ompF , mgtC , atpB , and sseB genes were deleted from the Salmonella chromosome using λ-Red recombination techniques as described previously [ , ].

    Article Title: Protein kinase A-dependent phosphorylation stimulates the transcriptional activity of hypoxia-inducible factor 1
    Article Snippet: Missense mutants of HIF-1α were generated by PCR using the Q5 Site-Directed Mutagenesis Kit and primers ( ) designed by NEBaseChanger software (New England Biolabs). .. His6 -R1a vector was generated by isolation of bovine R1a cDNA sequences from pRSETB (Addgene, plasmid 14922) as a HindIII/NdeI fragment and ligation to pET15b (Rapid DNA Ligation Kit, Thermo Scientific). .. Other plasmids have been previously described ( ).

    Software:

    Article Title: Protein kinase A-dependent phosphorylation stimulates the transcriptional activity of hypoxia-inducible factor 1
    Article Snippet: Missense mutants of HIF-1α were generated by PCR using the Q5 Site-Directed Mutagenesis Kit and primers ( ) designed by NEBaseChanger software (New England Biolabs). .. His6 -R1a vector was generated by isolation of bovine R1a cDNA sequences from pRSETB (Addgene, plasmid 14922) as a HindIII/NdeI fragment and ligation to pET15b (Rapid DNA Ligation Kit, Thermo Scientific).

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    Thermo Fisher rapid dna ligation kit
    Rapid Dna Ligation Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rapid dna ligation kit/product/Thermo Fisher
    Average 99 stars, based on 76 article reviews
    Price from $9.99 to $1999.99
    rapid dna ligation kit - by Bioz Stars, 2019-12
    99/100 stars
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