rapid cycle compatible phusion high fidelity polymerase  (New England Biolabs)


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    Structured Review

    New England Biolabs rapid cycle compatible phusion high fidelity polymerase
    E-Gel image showing sequential 26-cycle <t>Phusion</t> / GAPDH cleaning and decontamination experiments. Odd numbered lanes are identical positive controls preceded by cleaning protocol A. Lanes (M) 50 bp ladder, (2) NTC preceded by cleaning protocol A, (4) NTC preceded by cleaning protocol B, (6) NTC preceded by cleaning protocol C, (8) NTC preceded by cleaning protocol D, (10) NTC preceded by cleaning protocol E. The yellow arrow indicates the position of primer bands, while green arrows indicate faint nonspecific bands in the rzPCR positive control lanes.
    Rapid Cycle Compatible Phusion High Fidelity Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rapid cycle compatible phusion high fidelity polymerase/product/New England Biolabs
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rapid cycle compatible phusion high fidelity polymerase - by Bioz Stars, 2020-01
    80/100 stars

    Related Products / Commonly Used Together

    rapid single-plex amplification

    Images

    1) Product Images from "The Rotary Zone Thermal Cycler: A Low-Power System Enabling Automated Rapid PCR"

    Article Title: The Rotary Zone Thermal Cycler: A Low-Power System Enabling Automated Rapid PCR

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0118182

    E-Gel image showing sequential 26-cycle Phusion / GAPDH cleaning and decontamination experiments. Odd numbered lanes are identical positive controls preceded by cleaning protocol A. Lanes (M) 50 bp ladder, (2) NTC preceded by cleaning protocol A, (4) NTC preceded by cleaning protocol B, (6) NTC preceded by cleaning protocol C, (8) NTC preceded by cleaning protocol D, (10) NTC preceded by cleaning protocol E. The yellow arrow indicates the position of primer bands, while green arrows indicate faint nonspecific bands in the rzPCR positive control lanes.
    Figure Legend Snippet: E-Gel image showing sequential 26-cycle Phusion / GAPDH cleaning and decontamination experiments. Odd numbered lanes are identical positive controls preceded by cleaning protocol A. Lanes (M) 50 bp ladder, (2) NTC preceded by cleaning protocol A, (4) NTC preceded by cleaning protocol B, (6) NTC preceded by cleaning protocol C, (8) NTC preceded by cleaning protocol D, (10) NTC preceded by cleaning protocol E. The yellow arrow indicates the position of primer bands, while green arrows indicate faint nonspecific bands in the rzPCR positive control lanes.

    Techniques Used: Positive Control

    RZTC power and temperature history from startup for six Phusion rzPCR experiments with thermal cycling intervals indicated by the shaded bands. (A) Total (wall) power drawn by the heater control box during initial idle, warm-up, and cycling. (B) Detail of power fluctuations during cleaning, sample load, amplification, and sample unload. (C) Temperature fluctuations for heater blocks 2–4 about their set-points at 60°C, 72°C, and 98°C. (D) Temperature evolution of unheated block 1 over time.
    Figure Legend Snippet: RZTC power and temperature history from startup for six Phusion rzPCR experiments with thermal cycling intervals indicated by the shaded bands. (A) Total (wall) power drawn by the heater control box during initial idle, warm-up, and cycling. (B) Detail of power fluctuations during cleaning, sample load, amplification, and sample unload. (C) Temperature fluctuations for heater blocks 2–4 about their set-points at 60°C, 72°C, and 98°C. (D) Temperature evolution of unheated block 1 over time.

    Techniques Used: Amplification, Blocking Assay

    E-Gel image showing alternating positive control and no template control 26-cycle GAPDH / Phusion experiments. Lanes (M) 50 bp ladder, (1) Bench-top NTC, (2) Bench-top positive control, (3, 5, 7, 9) rzPCR NTC, (4, 6, 8, 10) rzPCR positive control. The yellow arrow indicates the position of primer bands, while green arrows indicate faint nonspecific bands in the rzPCR positive control lanes. Streaks and smudging are post-separation handling artifacts.
    Figure Legend Snippet: E-Gel image showing alternating positive control and no template control 26-cycle GAPDH / Phusion experiments. Lanes (M) 50 bp ladder, (1) Bench-top NTC, (2) Bench-top positive control, (3, 5, 7, 9) rzPCR NTC, (4, 6, 8, 10) rzPCR positive control. The yellow arrow indicates the position of primer bands, while green arrows indicate faint nonspecific bands in the rzPCR positive control lanes. Streaks and smudging are post-separation handling artifacts.

    Techniques Used: Positive Control

    Related Articles

    Flow Cytometry:

    Article Title: The Rotary Zone Thermal Cycler: A Low-Power System Enabling Automated Rapid PCR
    Article Snippet: Because the inlet cleaning operations already flush bleach and water through the reactor, protocol C abbreviates the dedicated wheel washing process with faster flow rates and shorter dwell times. .. Rapid Single-Plex Amplification: A protocol based on the rapid cycle-compatible Phusion high fidelity polymerase (New England Biolabs, Ipswitch, MA) was employed to evaluate the operation of the rzPCR system for single-plex PCR.

    Amplification:

    Article Title: The Rotary Zone Thermal Cycler: A Low-Power System Enabling Automated Rapid PCR
    Article Snippet: .. Rapid Single-Plex Amplification: A protocol based on the rapid cycle-compatible Phusion high fidelity polymerase (New England Biolabs, Ipswitch, MA) was employed to evaluate the operation of the rzPCR system for single-plex PCR. .. A custom 2357 base pair (bp) plasmid incorporating a 362 bp human GAPDH sequence (pIDTSMAR-AMP: GAPDH, Integrated DNA Technologies, Coralville, IA) tagged with primers corresponding to the Illumina sequencing adaptors, which commonly serves as an amplification standard in our labs, was used as the template for positive control experiments.

    Positive Control:

    Article Title: The Rotary Zone Thermal Cycler: A Low-Power System Enabling Automated Rapid PCR
    Article Snippet: Rapid Single-Plex Amplification: A protocol based on the rapid cycle-compatible Phusion high fidelity polymerase (New England Biolabs, Ipswitch, MA) was employed to evaluate the operation of the rzPCR system for single-plex PCR. .. A custom 2357 base pair (bp) plasmid incorporating a 362 bp human GAPDH sequence (pIDTSMAR-AMP: GAPDH, Integrated DNA Technologies, Coralville, IA) tagged with primers corresponding to the Illumina sequencing adaptors, which commonly serves as an amplification standard in our labs, was used as the template for positive control experiments.

    Polymerase Chain Reaction:

    Article Title: The Rotary Zone Thermal Cycler: A Low-Power System Enabling Automated Rapid PCR
    Article Snippet: .. Rapid Single-Plex Amplification: A protocol based on the rapid cycle-compatible Phusion high fidelity polymerase (New England Biolabs, Ipswitch, MA) was employed to evaluate the operation of the rzPCR system for single-plex PCR. .. A custom 2357 base pair (bp) plasmid incorporating a 362 bp human GAPDH sequence (pIDTSMAR-AMP: GAPDH, Integrated DNA Technologies, Coralville, IA) tagged with primers corresponding to the Illumina sequencing adaptors, which commonly serves as an amplification standard in our labs, was used as the template for positive control experiments.

    Sequencing:

    Article Title: The Rotary Zone Thermal Cycler: A Low-Power System Enabling Automated Rapid PCR
    Article Snippet: Rapid Single-Plex Amplification: A protocol based on the rapid cycle-compatible Phusion high fidelity polymerase (New England Biolabs, Ipswitch, MA) was employed to evaluate the operation of the rzPCR system for single-plex PCR. .. A custom 2357 base pair (bp) plasmid incorporating a 362 bp human GAPDH sequence (pIDTSMAR-AMP: GAPDH, Integrated DNA Technologies, Coralville, IA) tagged with primers corresponding to the Illumina sequencing adaptors, which commonly serves as an amplification standard in our labs, was used as the template for positive control experiments.

    IA:

    Article Title: The Rotary Zone Thermal Cycler: A Low-Power System Enabling Automated Rapid PCR
    Article Snippet: Rapid Single-Plex Amplification: A protocol based on the rapid cycle-compatible Phusion high fidelity polymerase (New England Biolabs, Ipswitch, MA) was employed to evaluate the operation of the rzPCR system for single-plex PCR. .. A custom 2357 base pair (bp) plasmid incorporating a 362 bp human GAPDH sequence (pIDTSMAR-AMP: GAPDH, Integrated DNA Technologies, Coralville, IA) tagged with primers corresponding to the Illumina sequencing adaptors, which commonly serves as an amplification standard in our labs, was used as the template for positive control experiments.

    Plasmid Preparation:

    Article Title: The Rotary Zone Thermal Cycler: A Low-Power System Enabling Automated Rapid PCR
    Article Snippet: Rapid Single-Plex Amplification: A protocol based on the rapid cycle-compatible Phusion high fidelity polymerase (New England Biolabs, Ipswitch, MA) was employed to evaluate the operation of the rzPCR system for single-plex PCR. .. A custom 2357 base pair (bp) plasmid incorporating a 362 bp human GAPDH sequence (pIDTSMAR-AMP: GAPDH, Integrated DNA Technologies, Coralville, IA) tagged with primers corresponding to the Illumina sequencing adaptors, which commonly serves as an amplification standard in our labs, was used as the template for positive control experiments.

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  • 80
    New England Biolabs rapid cycle compatible phusion high fidelity polymerase
    E-Gel image showing sequential 26-cycle <t>Phusion</t> / GAPDH cleaning and decontamination experiments. Odd numbered lanes are identical positive controls preceded by cleaning protocol A. Lanes (M) 50 bp ladder, (2) NTC preceded by cleaning protocol A, (4) NTC preceded by cleaning protocol B, (6) NTC preceded by cleaning protocol C, (8) NTC preceded by cleaning protocol D, (10) NTC preceded by cleaning protocol E. The yellow arrow indicates the position of primer bands, while green arrows indicate faint nonspecific bands in the rzPCR positive control lanes.
    Rapid Cycle Compatible Phusion High Fidelity Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rapid cycle compatible phusion high fidelity polymerase/product/New England Biolabs
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rapid cycle compatible phusion high fidelity polymerase - by Bioz Stars, 2020-01
    80/100 stars
      Buy from Supplier

    Image Search Results


    E-Gel image showing sequential 26-cycle Phusion / GAPDH cleaning and decontamination experiments. Odd numbered lanes are identical positive controls preceded by cleaning protocol A. Lanes (M) 50 bp ladder, (2) NTC preceded by cleaning protocol A, (4) NTC preceded by cleaning protocol B, (6) NTC preceded by cleaning protocol C, (8) NTC preceded by cleaning protocol D, (10) NTC preceded by cleaning protocol E. The yellow arrow indicates the position of primer bands, while green arrows indicate faint nonspecific bands in the rzPCR positive control lanes.

    Journal: PLoS ONE

    Article Title: The Rotary Zone Thermal Cycler: A Low-Power System Enabling Automated Rapid PCR

    doi: 10.1371/journal.pone.0118182

    Figure Lengend Snippet: E-Gel image showing sequential 26-cycle Phusion / GAPDH cleaning and decontamination experiments. Odd numbered lanes are identical positive controls preceded by cleaning protocol A. Lanes (M) 50 bp ladder, (2) NTC preceded by cleaning protocol A, (4) NTC preceded by cleaning protocol B, (6) NTC preceded by cleaning protocol C, (8) NTC preceded by cleaning protocol D, (10) NTC preceded by cleaning protocol E. The yellow arrow indicates the position of primer bands, while green arrows indicate faint nonspecific bands in the rzPCR positive control lanes.

    Article Snippet: Rapid Single-Plex Amplification: A protocol based on the rapid cycle-compatible Phusion high fidelity polymerase (New England Biolabs, Ipswitch, MA) was employed to evaluate the operation of the rzPCR system for single-plex PCR.

    Techniques: Positive Control

    RZTC power and temperature history from startup for six Phusion rzPCR experiments with thermal cycling intervals indicated by the shaded bands. (A) Total (wall) power drawn by the heater control box during initial idle, warm-up, and cycling. (B) Detail of power fluctuations during cleaning, sample load, amplification, and sample unload. (C) Temperature fluctuations for heater blocks 2–4 about their set-points at 60°C, 72°C, and 98°C. (D) Temperature evolution of unheated block 1 over time.

    Journal: PLoS ONE

    Article Title: The Rotary Zone Thermal Cycler: A Low-Power System Enabling Automated Rapid PCR

    doi: 10.1371/journal.pone.0118182

    Figure Lengend Snippet: RZTC power and temperature history from startup for six Phusion rzPCR experiments with thermal cycling intervals indicated by the shaded bands. (A) Total (wall) power drawn by the heater control box during initial idle, warm-up, and cycling. (B) Detail of power fluctuations during cleaning, sample load, amplification, and sample unload. (C) Temperature fluctuations for heater blocks 2–4 about their set-points at 60°C, 72°C, and 98°C. (D) Temperature evolution of unheated block 1 over time.

    Article Snippet: Rapid Single-Plex Amplification: A protocol based on the rapid cycle-compatible Phusion high fidelity polymerase (New England Biolabs, Ipswitch, MA) was employed to evaluate the operation of the rzPCR system for single-plex PCR.

    Techniques: Amplification, Blocking Assay

    E-Gel image showing alternating positive control and no template control 26-cycle GAPDH / Phusion experiments. Lanes (M) 50 bp ladder, (1) Bench-top NTC, (2) Bench-top positive control, (3, 5, 7, 9) rzPCR NTC, (4, 6, 8, 10) rzPCR positive control. The yellow arrow indicates the position of primer bands, while green arrows indicate faint nonspecific bands in the rzPCR positive control lanes. Streaks and smudging are post-separation handling artifacts.

    Journal: PLoS ONE

    Article Title: The Rotary Zone Thermal Cycler: A Low-Power System Enabling Automated Rapid PCR

    doi: 10.1371/journal.pone.0118182

    Figure Lengend Snippet: E-Gel image showing alternating positive control and no template control 26-cycle GAPDH / Phusion experiments. Lanes (M) 50 bp ladder, (1) Bench-top NTC, (2) Bench-top positive control, (3, 5, 7, 9) rzPCR NTC, (4, 6, 8, 10) rzPCR positive control. The yellow arrow indicates the position of primer bands, while green arrows indicate faint nonspecific bands in the rzPCR positive control lanes. Streaks and smudging are post-separation handling artifacts.

    Article Snippet: Rapid Single-Plex Amplification: A protocol based on the rapid cycle-compatible Phusion high fidelity polymerase (New England Biolabs, Ipswitch, MA) was employed to evaluate the operation of the rzPCR system for single-plex PCR.

    Techniques: Positive Control