rapid cycle compatible phusion high fidelity polymerase  (New England Biolabs)


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    Structured Review

    New England Biolabs rapid cycle compatible phusion high fidelity polymerase
    E-Gel image showing sequential 26-cycle <t>Phusion</t> / GAPDH cleaning and decontamination experiments. Odd numbered lanes are identical positive controls preceded by cleaning protocol A. Lanes (M) 50 bp ladder, (2) NTC preceded by cleaning protocol A, (4) NTC preceded by cleaning protocol B, (6) NTC preceded by cleaning protocol C, (8) NTC preceded by cleaning protocol D, (10) NTC preceded by cleaning protocol E. The yellow arrow indicates the position of primer bands, while green arrows indicate faint nonspecific bands in the rzPCR positive control lanes.
    Rapid Cycle Compatible Phusion High Fidelity Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rapid cycle compatible phusion high fidelity polymerase/product/New England Biolabs
    Average 99 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    rapid cycle compatible phusion high fidelity polymerase - by Bioz Stars, 2020-09
    99/100 stars

    Images

    1) Product Images from "The Rotary Zone Thermal Cycler: A Low-Power System Enabling Automated Rapid PCR"

    Article Title: The Rotary Zone Thermal Cycler: A Low-Power System Enabling Automated Rapid PCR

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0118182

    E-Gel image showing sequential 26-cycle Phusion / GAPDH cleaning and decontamination experiments. Odd numbered lanes are identical positive controls preceded by cleaning protocol A. Lanes (M) 50 bp ladder, (2) NTC preceded by cleaning protocol A, (4) NTC preceded by cleaning protocol B, (6) NTC preceded by cleaning protocol C, (8) NTC preceded by cleaning protocol D, (10) NTC preceded by cleaning protocol E. The yellow arrow indicates the position of primer bands, while green arrows indicate faint nonspecific bands in the rzPCR positive control lanes.
    Figure Legend Snippet: E-Gel image showing sequential 26-cycle Phusion / GAPDH cleaning and decontamination experiments. Odd numbered lanes are identical positive controls preceded by cleaning protocol A. Lanes (M) 50 bp ladder, (2) NTC preceded by cleaning protocol A, (4) NTC preceded by cleaning protocol B, (6) NTC preceded by cleaning protocol C, (8) NTC preceded by cleaning protocol D, (10) NTC preceded by cleaning protocol E. The yellow arrow indicates the position of primer bands, while green arrows indicate faint nonspecific bands in the rzPCR positive control lanes.

    Techniques Used: Positive Control

    RZTC power and temperature history from startup for six Phusion rzPCR experiments with thermal cycling intervals indicated by the shaded bands. (A) Total (wall) power drawn by the heater control box during initial idle, warm-up, and cycling. (B) Detail of power fluctuations during cleaning, sample load, amplification, and sample unload. (C) Temperature fluctuations for heater blocks 2–4 about their set-points at 60°C, 72°C, and 98°C. (D) Temperature evolution of unheated block 1 over time.
    Figure Legend Snippet: RZTC power and temperature history from startup for six Phusion rzPCR experiments with thermal cycling intervals indicated by the shaded bands. (A) Total (wall) power drawn by the heater control box during initial idle, warm-up, and cycling. (B) Detail of power fluctuations during cleaning, sample load, amplification, and sample unload. (C) Temperature fluctuations for heater blocks 2–4 about their set-points at 60°C, 72°C, and 98°C. (D) Temperature evolution of unheated block 1 over time.

    Techniques Used: Amplification, Blocking Assay

    E-Gel image showing alternating positive control and no template control 26-cycle GAPDH / Phusion experiments. Lanes (M) 50 bp ladder, (1) Bench-top NTC, (2) Bench-top positive control, (3, 5, 7, 9) rzPCR NTC, (4, 6, 8, 10) rzPCR positive control. The yellow arrow indicates the position of primer bands, while green arrows indicate faint nonspecific bands in the rzPCR positive control lanes. Streaks and smudging are post-separation handling artifacts.
    Figure Legend Snippet: E-Gel image showing alternating positive control and no template control 26-cycle GAPDH / Phusion experiments. Lanes (M) 50 bp ladder, (1) Bench-top NTC, (2) Bench-top positive control, (3, 5, 7, 9) rzPCR NTC, (4, 6, 8, 10) rzPCR positive control. The yellow arrow indicates the position of primer bands, while green arrows indicate faint nonspecific bands in the rzPCR positive control lanes. Streaks and smudging are post-separation handling artifacts.

    Techniques Used: Positive Control

    Related Articles

    Clone Assay:

    Article Title: Functional Dissection of the Human TNRC6 (GW182-Related) Family of Proteins ▿Functional Dissection of the Human TNRC6 (GW182-Related) Family of Proteins ▿ †
    Article Snippet: .. Full-length Ago1 was amplified by standard PCR techniques using vent polymerase (New England Biolabs) and cloned between the EcoRI and BamHI restriction sites of the pFlag-CMV2 (Sigma) vector. .. MS2 fusion constructs were generated by PCR amplification of the MS2-binding domain and cloning into the HindIII restriction site of the different pFlag-CMV2 constructs.

    Amplification:

    Article Title: Functional Dissection of the Human TNRC6 (GW182-Related) Family of Proteins ▿Functional Dissection of the Human TNRC6 (GW182-Related) Family of Proteins ▿ †
    Article Snippet: .. Full-length Ago1 was amplified by standard PCR techniques using vent polymerase (New England Biolabs) and cloned between the EcoRI and BamHI restriction sites of the pFlag-CMV2 (Sigma) vector. .. MS2 fusion constructs were generated by PCR amplification of the MS2-binding domain and cloning into the HindIII restriction site of the different pFlag-CMV2 constructs.

    Article Title: Circularization pathway of a bacterial group II intron
    Article Snippet: .. For Ll.LtrB-ΔA a second amplification of the 3′ end was performed where a polyU instead of a polyA tail was added using 2U of Poly(U) Polymerase (New England Biolabs) (10 min at 37°C). ..

    Article Title: Molecular and Biological Characterization of a New Strawberry Cytorhabdovirus
    Article Snippet: .. For 3′ terminal amplification, a prior polyadenylation of total RNA with polyU polymerase (NEB, Ipswich, MA, USA) was performed following the manufacturer’s recommendations. .. The PCR products thus obtained were resolved on 1% agarose gels, stained with GelRed (Biotium, Hayward, CA, USA), and photographed under UV illumination.

    Synthesized:

    Article Title: Conformational Dynamics of the HIV-Vif Protein Complex
    Article Snippet: .. RNase protection Body-labeled poly(U) RNA was synthesized by a tailing reaction with poly(U) polymerase (New England Biolabs), poly(U)15 oligonucleotide primer, and [ α -32 P] UTP. .. Long RNAs were purified using an RNeasy Mini Spin Column (QIAGEN, Hilden, Germany) according to the manufacturer’s recommendations.

    Quantitation Assay:

    Article Title: Decreased miR-155-5p, miR-15a, and miR-186 Expression in Gastric Cancer Is Associated with Advanced Tumor Grade and Metastasis
    Article Snippet: .. MiRNA quantitation Briefly, a poly-A tail was added to total RNAs (500 ng) using Poly-A polymerase (New England Biolabs, UK) at 37 °C for 30 min according to manufacturer’s protocol. .. For cDNA synthesis, we used PrimeScript™ 1st strand cDNA Synthesis Kit (TaKaRa, Japan) and an RT adaptor primer ( ).

    Produced:

    Article Title: RNA-DNA strand exchange by the Drosophila Polycomb complex PRC2
    Article Snippet: .. Radiolabelled RNAs were produced from circular templates by transcribing 600 ng of DNA in RNA polymerase buffer (NEB), 1 mM DTT, 625 µM of rNTP (NEB), 6.5 nmol of radiolabelled UTP, 200 U of RNase inhibitor, and 250 U of T7 or T3 RNA polymerases (NEB) in 100 µL O.N. at 37 °C. .. DNA was removed from the reaction by adding 4 U of DNaseI (NEB) and incubating 2 h at 37 °C.

    Incubation:

    Article Title: Hallmarks of Hepatitis C Virus in Equine Hepacivirus
    Article Snippet: .. The poly(U) tail was added to the 3′ end of the RNA preparation using Escherichia coli poly(U) polymerase (New England BioLabs, Ipswich, MA) and was incubated for 45 min at 37°C. .. The resulting preparation was reverse transcribed by the SuperScript First-Strand Synthesis system (Life Technologies) using an oligo(dA) adapter primer (5′-TTGCGAGCACAGAATTAATACGACTCACAAAAAAAAAAAAVN-3′).

    Polymerase Chain Reaction:

    Article Title: Functional Dissection of the Human TNRC6 (GW182-Related) Family of Proteins ▿Functional Dissection of the Human TNRC6 (GW182-Related) Family of Proteins ▿ †
    Article Snippet: .. Full-length Ago1 was amplified by standard PCR techniques using vent polymerase (New England Biolabs) and cloned between the EcoRI and BamHI restriction sites of the pFlag-CMV2 (Sigma) vector. .. MS2 fusion constructs were generated by PCR amplification of the MS2-binding domain and cloning into the HindIII restriction site of the different pFlag-CMV2 constructs.

    Plasmid Preparation:

    Article Title: Functional Dissection of the Human TNRC6 (GW182-Related) Family of Proteins ▿Functional Dissection of the Human TNRC6 (GW182-Related) Family of Proteins ▿ †
    Article Snippet: .. Full-length Ago1 was amplified by standard PCR techniques using vent polymerase (New England Biolabs) and cloned between the EcoRI and BamHI restriction sites of the pFlag-CMV2 (Sigma) vector. .. MS2 fusion constructs were generated by PCR amplification of the MS2-binding domain and cloning into the HindIII restriction site of the different pFlag-CMV2 constructs.

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  • 99
    New England Biolabs rapid cycle compatible phusion high fidelity polymerase
    E-Gel image showing sequential 26-cycle <t>Phusion</t> / GAPDH cleaning and decontamination experiments. Odd numbered lanes are identical positive controls preceded by cleaning protocol A. Lanes (M) 50 bp ladder, (2) NTC preceded by cleaning protocol A, (4) NTC preceded by cleaning protocol B, (6) NTC preceded by cleaning protocol C, (8) NTC preceded by cleaning protocol D, (10) NTC preceded by cleaning protocol E. The yellow arrow indicates the position of primer bands, while green arrows indicate faint nonspecific bands in the rzPCR positive control lanes.
    Rapid Cycle Compatible Phusion High Fidelity Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rapid cycle compatible phusion high fidelity polymerase/product/New England Biolabs
    Average 99 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    rapid cycle compatible phusion high fidelity polymerase - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    E-Gel image showing sequential 26-cycle Phusion / GAPDH cleaning and decontamination experiments. Odd numbered lanes are identical positive controls preceded by cleaning protocol A. Lanes (M) 50 bp ladder, (2) NTC preceded by cleaning protocol A, (4) NTC preceded by cleaning protocol B, (6) NTC preceded by cleaning protocol C, (8) NTC preceded by cleaning protocol D, (10) NTC preceded by cleaning protocol E. The yellow arrow indicates the position of primer bands, while green arrows indicate faint nonspecific bands in the rzPCR positive control lanes.

    Journal: PLoS ONE

    Article Title: The Rotary Zone Thermal Cycler: A Low-Power System Enabling Automated Rapid PCR

    doi: 10.1371/journal.pone.0118182

    Figure Lengend Snippet: E-Gel image showing sequential 26-cycle Phusion / GAPDH cleaning and decontamination experiments. Odd numbered lanes are identical positive controls preceded by cleaning protocol A. Lanes (M) 50 bp ladder, (2) NTC preceded by cleaning protocol A, (4) NTC preceded by cleaning protocol B, (6) NTC preceded by cleaning protocol C, (8) NTC preceded by cleaning protocol D, (10) NTC preceded by cleaning protocol E. The yellow arrow indicates the position of primer bands, while green arrows indicate faint nonspecific bands in the rzPCR positive control lanes.

    Article Snippet: Rapid Single-Plex Amplification: A protocol based on the rapid cycle-compatible Phusion high fidelity polymerase (New England Biolabs, Ipswitch, MA) was employed to evaluate the operation of the rzPCR system for single-plex PCR.

    Techniques: Positive Control

    RZTC power and temperature history from startup for six Phusion rzPCR experiments with thermal cycling intervals indicated by the shaded bands. (A) Total (wall) power drawn by the heater control box during initial idle, warm-up, and cycling. (B) Detail of power fluctuations during cleaning, sample load, amplification, and sample unload. (C) Temperature fluctuations for heater blocks 2–4 about their set-points at 60°C, 72°C, and 98°C. (D) Temperature evolution of unheated block 1 over time.

    Journal: PLoS ONE

    Article Title: The Rotary Zone Thermal Cycler: A Low-Power System Enabling Automated Rapid PCR

    doi: 10.1371/journal.pone.0118182

    Figure Lengend Snippet: RZTC power and temperature history from startup for six Phusion rzPCR experiments with thermal cycling intervals indicated by the shaded bands. (A) Total (wall) power drawn by the heater control box during initial idle, warm-up, and cycling. (B) Detail of power fluctuations during cleaning, sample load, amplification, and sample unload. (C) Temperature fluctuations for heater blocks 2–4 about their set-points at 60°C, 72°C, and 98°C. (D) Temperature evolution of unheated block 1 over time.

    Article Snippet: Rapid Single-Plex Amplification: A protocol based on the rapid cycle-compatible Phusion high fidelity polymerase (New England Biolabs, Ipswitch, MA) was employed to evaluate the operation of the rzPCR system for single-plex PCR.

    Techniques: Amplification, Blocking Assay

    E-Gel image showing alternating positive control and no template control 26-cycle GAPDH / Phusion experiments. Lanes (M) 50 bp ladder, (1) Bench-top NTC, (2) Bench-top positive control, (3, 5, 7, 9) rzPCR NTC, (4, 6, 8, 10) rzPCR positive control. The yellow arrow indicates the position of primer bands, while green arrows indicate faint nonspecific bands in the rzPCR positive control lanes. Streaks and smudging are post-separation handling artifacts.

    Journal: PLoS ONE

    Article Title: The Rotary Zone Thermal Cycler: A Low-Power System Enabling Automated Rapid PCR

    doi: 10.1371/journal.pone.0118182

    Figure Lengend Snippet: E-Gel image showing alternating positive control and no template control 26-cycle GAPDH / Phusion experiments. Lanes (M) 50 bp ladder, (1) Bench-top NTC, (2) Bench-top positive control, (3, 5, 7, 9) rzPCR NTC, (4, 6, 8, 10) rzPCR positive control. The yellow arrow indicates the position of primer bands, while green arrows indicate faint nonspecific bands in the rzPCR positive control lanes. Streaks and smudging are post-separation handling artifacts.

    Article Snippet: Rapid Single-Plex Amplification: A protocol based on the rapid cycle-compatible Phusion high fidelity polymerase (New England Biolabs, Ipswitch, MA) was employed to evaluate the operation of the rzPCR system for single-plex PCR.

    Techniques: Positive Control