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Boehringer Mannheim random primed dna dig labelling kit
Confirmation of gene disruption by Southern analysis. Lane 1, <t>DNA</t> from the control strain; lane 2, DNA from the disrupted strain; lane 3, Dig-labelled DNA molecular weight marker III (Boehringer <t>Mannheim).</t>
Random Primed Dna Dig Labelling Kit, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/random primed dna dig labelling kit/product/Boehringer Mannheim
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random primed dna dig labelling kit - by Bioz Stars, 2021-04
86/100 stars

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1) Product Images from "Isolation, Nucleotide Sequence, and Physiological Relevance of the Gene Encoding Triose Phosphate Isomerase from Kluyveromyces lactis"

Article Title: Isolation, Nucleotide Sequence, and Physiological Relevance of the Gene Encoding Triose Phosphate Isomerase from Kluyveromyces lactis

Journal: Applied and Environmental Microbiology

doi:

Confirmation of gene disruption by Southern analysis. Lane 1, DNA from the control strain; lane 2, DNA from the disrupted strain; lane 3, Dig-labelled DNA molecular weight marker III (Boehringer Mannheim).
Figure Legend Snippet: Confirmation of gene disruption by Southern analysis. Lane 1, DNA from the control strain; lane 2, DNA from the disrupted strain; lane 3, Dig-labelled DNA molecular weight marker III (Boehringer Mannheim).

Techniques Used: Molecular Weight, Marker

Related Articles

Synthesized:

Article Title: Identification of genes differentially expressed during larval molting and metamorphosis of Helicoverpa armigera
Article Snippet: Preparation of digoxigenin-labeled DNA probes Complementary DNAs (cDNA) synthesized from mRNAs isolated from 5th and 6th instar epidermis were used as templates for synthesizing dig-labeled DNA probes. .. The probes were synthesized by random primed PCR using the Dig High Prime DNA Labeling and Detection Starter Kit (Boehringer Mannheim, Mannheim, Germany). .. Dot blot hybridization PCR products over 300 bp obtained by SSH were cloned into pGEM T-Easy plasmid and transformed into DH5α.

Random Primed:

Article Title: Identification of genes differentially expressed during larval molting and metamorphosis of Helicoverpa armigera
Article Snippet: Preparation of digoxigenin-labeled DNA probes Complementary DNAs (cDNA) synthesized from mRNAs isolated from 5th and 6th instar epidermis were used as templates for synthesizing dig-labeled DNA probes. .. The probes were synthesized by random primed PCR using the Dig High Prime DNA Labeling and Detection Starter Kit (Boehringer Mannheim, Mannheim, Germany). .. Dot blot hybridization PCR products over 300 bp obtained by SSH were cloned into pGEM T-Easy plasmid and transformed into DH5α.

Article Title: Transcriptional Analysis of Essential Genes of the Escherichia coli Fatty Acid Biosynthesis Gene Cluster by Functional Replacement with the Analogous Salmonella typhimurium Gene Cluster
Article Snippet: Southern blot analyses were carried out according to the Genius System User’s Guide (Boehringer Mannheim Biochemicals). .. The probes were plasmid pYZ37, pYZ47, and pYZ69 labeled with digoxigenin (DIG)-dUTP via random-primed labeling with the Genius 2 DNA labeling kit, purchased from Boehringer Mannheim. .. S. typhimurium Mu d -P22 phage lysate preparation and DNA isolation were performed according to the procedure of Youderian and coworkers ( ).

Article Title: Purification and Partial Characterization of an Entomopoxvirus (DlEPV) from a Parasitic Wasp of Tephritid Fruit Flies.
Article Snippet: .. Random Primed Labeling of DlEPV DNA Probe Viral DNA was denatured by boiling then DIG-labeled by random priming, using the Genius 1 kit (Boehringer-Mannheim). .. DNA was incubated at 37°C for 16 h in the reagents (hexanucleotide mix, dNTP labeling mix, sterile distilled water and Klenow enzyme) provided by the manufacturer, and the reaction was terminated by the addition of 200 mM EDTA (pH 8.0).

Article Title: Clusters of transcription-coupled repair in the human genome
Article Snippet: The fractions containing the bound (i.e., immunoextracted) DNA fragments (repaired DNA) and the nonbound fragments (unrepaired DNA) were neutralized, and the concentration of human DNA in both fractions was measured for all cell lines by slot blot hybridization with radioactively labeled human DNA; the specificity of the immunoextraction was then tested by hybridization with strand-specific DNA probes of the human adenosine deaminase gene, as described ( ). .. Repaired (bound) and unrepaired (nonbound) DNA fractions were directly labeled with digoxigenin-11-dUTP by random primed reactions (DIG-High Prime kit, Boehringer Mannheim) or simultaneously amplified and labeled with biotin-16-dUTP by degenerate oligonucleotide primed (DOP)-PCR. ..

Article Title: A Chromatin-associated Kinesin-related Protein Required for Normal Mitotic Chromosome Segregation in Drosophila
Article Snippet: Northern blot analyses were carried out as described in reference . .. DNA probes were either digoxigenin-labeled by the random primed method using the DIG-DNA labeling kit ( Boehringer Mannheim Corp. ) or radioactively labeled by random priming. .. Antisense riboprobes were radioactively labeled during in vitro transcription from cDNA clones or subclones as in reference .

Article Title: Isolation, Nucleotide Sequence, and Physiological Relevance of the Gene Encoding Triose Phosphate Isomerase from Kluyveromyces lactis
Article Snippet: The DNA was digested with restriction endonuclease Csp 45 I, separated on agarose gel, and blotted onto a nylon membrane (Hybond-N; Amersham), as suggested by suppliers. .. Hybridization was performed by using as a probe the Bgl II- Csp 45 I fragment, Dig-labelled (Random Primed DNA Dig-labelling kit; Boehringer Mannheim) at 48°C in 50% formamide, 5× SSC, 0.2% SDS, and 2.5% blocking solution. ..

Polymerase Chain Reaction:

Article Title: Identification of genes differentially expressed during larval molting and metamorphosis of Helicoverpa armigera
Article Snippet: Preparation of digoxigenin-labeled DNA probes Complementary DNAs (cDNA) synthesized from mRNAs isolated from 5th and 6th instar epidermis were used as templates for synthesizing dig-labeled DNA probes. .. The probes were synthesized by random primed PCR using the Dig High Prime DNA Labeling and Detection Starter Kit (Boehringer Mannheim, Mannheim, Germany). .. Dot blot hybridization PCR products over 300 bp obtained by SSH were cloned into pGEM T-Easy plasmid and transformed into DH5α.

DNA Labeling:

Article Title: Identification of genes differentially expressed during larval molting and metamorphosis of Helicoverpa armigera
Article Snippet: Preparation of digoxigenin-labeled DNA probes Complementary DNAs (cDNA) synthesized from mRNAs isolated from 5th and 6th instar epidermis were used as templates for synthesizing dig-labeled DNA probes. .. The probes were synthesized by random primed PCR using the Dig High Prime DNA Labeling and Detection Starter Kit (Boehringer Mannheim, Mannheim, Germany). .. Dot blot hybridization PCR products over 300 bp obtained by SSH were cloned into pGEM T-Easy plasmid and transformed into DH5α.

Article Title: Transcriptional Analysis of Essential Genes of the Escherichia coli Fatty Acid Biosynthesis Gene Cluster by Functional Replacement with the Analogous Salmonella typhimurium Gene Cluster
Article Snippet: Southern blot analyses were carried out according to the Genius System User’s Guide (Boehringer Mannheim Biochemicals). .. The probes were plasmid pYZ37, pYZ47, and pYZ69 labeled with digoxigenin (DIG)-dUTP via random-primed labeling with the Genius 2 DNA labeling kit, purchased from Boehringer Mannheim. .. S. typhimurium Mu d -P22 phage lysate preparation and DNA isolation were performed according to the procedure of Youderian and coworkers ( ).

Plasmid Preparation:

Article Title: Transcriptional Analysis of Essential Genes of the Escherichia coli Fatty Acid Biosynthesis Gene Cluster by Functional Replacement with the Analogous Salmonella typhimurium Gene Cluster
Article Snippet: Southern blot analyses were carried out according to the Genius System User’s Guide (Boehringer Mannheim Biochemicals). .. The probes were plasmid pYZ37, pYZ47, and pYZ69 labeled with digoxigenin (DIG)-dUTP via random-primed labeling with the Genius 2 DNA labeling kit, purchased from Boehringer Mannheim. .. S. typhimurium Mu d -P22 phage lysate preparation and DNA isolation were performed according to the procedure of Youderian and coworkers ( ).

Labeling:

Article Title: Transcriptional Analysis of Essential Genes of the Escherichia coli Fatty Acid Biosynthesis Gene Cluster by Functional Replacement with the Analogous Salmonella typhimurium Gene Cluster
Article Snippet: Southern blot analyses were carried out according to the Genius System User’s Guide (Boehringer Mannheim Biochemicals). .. The probes were plasmid pYZ37, pYZ47, and pYZ69 labeled with digoxigenin (DIG)-dUTP via random-primed labeling with the Genius 2 DNA labeling kit, purchased from Boehringer Mannheim. .. S. typhimurium Mu d -P22 phage lysate preparation and DNA isolation were performed according to the procedure of Youderian and coworkers ( ).

Article Title: Clusters of transcription-coupled repair in the human genome
Article Snippet: The fractions containing the bound (i.e., immunoextracted) DNA fragments (repaired DNA) and the nonbound fragments (unrepaired DNA) were neutralized, and the concentration of human DNA in both fractions was measured for all cell lines by slot blot hybridization with radioactively labeled human DNA; the specificity of the immunoextraction was then tested by hybridization with strand-specific DNA probes of the human adenosine deaminase gene, as described ( ). .. Repaired (bound) and unrepaired (nonbound) DNA fractions were directly labeled with digoxigenin-11-dUTP by random primed reactions (DIG-High Prime kit, Boehringer Mannheim) or simultaneously amplified and labeled with biotin-16-dUTP by degenerate oligonucleotide primed (DOP)-PCR. ..

Article Title: A Chromatin-associated Kinesin-related Protein Required for Normal Mitotic Chromosome Segregation in Drosophila
Article Snippet: Northern blot analyses were carried out as described in reference . .. DNA probes were either digoxigenin-labeled by the random primed method using the DIG-DNA labeling kit ( Boehringer Mannheim Corp. ) or radioactively labeled by random priming. .. Antisense riboprobes were radioactively labeled during in vitro transcription from cDNA clones or subclones as in reference .

Amplification:

Article Title: Clusters of transcription-coupled repair in the human genome
Article Snippet: The fractions containing the bound (i.e., immunoextracted) DNA fragments (repaired DNA) and the nonbound fragments (unrepaired DNA) were neutralized, and the concentration of human DNA in both fractions was measured for all cell lines by slot blot hybridization with radioactively labeled human DNA; the specificity of the immunoextraction was then tested by hybridization with strand-specific DNA probes of the human adenosine deaminase gene, as described ( ). .. Repaired (bound) and unrepaired (nonbound) DNA fractions were directly labeled with digoxigenin-11-dUTP by random primed reactions (DIG-High Prime kit, Boehringer Mannheim) or simultaneously amplified and labeled with biotin-16-dUTP by degenerate oligonucleotide primed (DOP)-PCR. ..

Degenerate Oligonucleotide–primed Polymerase Chain Reaction:

Article Title: Clusters of transcription-coupled repair in the human genome
Article Snippet: The fractions containing the bound (i.e., immunoextracted) DNA fragments (repaired DNA) and the nonbound fragments (unrepaired DNA) were neutralized, and the concentration of human DNA in both fractions was measured for all cell lines by slot blot hybridization with radioactively labeled human DNA; the specificity of the immunoextraction was then tested by hybridization with strand-specific DNA probes of the human adenosine deaminase gene, as described ( ). .. Repaired (bound) and unrepaired (nonbound) DNA fractions were directly labeled with digoxigenin-11-dUTP by random primed reactions (DIG-High Prime kit, Boehringer Mannheim) or simultaneously amplified and labeled with biotin-16-dUTP by degenerate oligonucleotide primed (DOP)-PCR. ..

Hybridization:

Article Title: Isolation, Nucleotide Sequence, and Physiological Relevance of the Gene Encoding Triose Phosphate Isomerase from Kluyveromyces lactis
Article Snippet: The DNA was digested with restriction endonuclease Csp 45 I, separated on agarose gel, and blotted onto a nylon membrane (Hybond-N; Amersham), as suggested by suppliers. .. Hybridization was performed by using as a probe the Bgl II- Csp 45 I fragment, Dig-labelled (Random Primed DNA Dig-labelling kit; Boehringer Mannheim) at 48°C in 50% formamide, 5× SSC, 0.2% SDS, and 2.5% blocking solution. ..

Blocking Assay:

Article Title: Isolation, Nucleotide Sequence, and Physiological Relevance of the Gene Encoding Triose Phosphate Isomerase from Kluyveromyces lactis
Article Snippet: The DNA was digested with restriction endonuclease Csp 45 I, separated on agarose gel, and blotted onto a nylon membrane (Hybond-N; Amersham), as suggested by suppliers. .. Hybridization was performed by using as a probe the Bgl II- Csp 45 I fragment, Dig-labelled (Random Primed DNA Dig-labelling kit; Boehringer Mannheim) at 48°C in 50% formamide, 5× SSC, 0.2% SDS, and 2.5% blocking solution. ..

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    Boehringer Mannheim dig dna labeling kit
    Molecular organization of the tio gene. ( A ) Top line: Restriction map of the 20 kb of genomic <t>DNA</t> containing tio . Lower lines: Exon/intron map of the 3.6-kb KLP38B transcript and the 1.8-kb transcript contained within its large intron. The tio 1 P element is inserted within the intron of the 3.6-kb transcript, in a 700-bp PvuII-HindIII fragment upstream of the 5′ end of the cDNA representing the 1.8-kb transcript. The tio 24-0 (*) and tio 93-E (**) inserts were mapped to the intron of the 3.6-kb transcript by Alphey et al. ( 3 ), roughly as shown by the asterisks. The exact relationship of the tio 24-0 and tio 93-E inserts to the position of the intronic transcript was not published in Alphey et al. ( 3 ). ( B ) Developmental Northern probed with a cloned genomic fragment showing the two transcripts (3.6 and 1.8 kb) derived from the genomic DNA flanking the tio 1 P element insert. Both transcripts were present in all stages studied, although the 3.6-kb transcript was more abundant in 0–2-h embryos and the 1.8-kb transcript was more abundant in 4–8-h embryos. ( C and D ) Northern analysis of polyA + RNA from third instar larvae of the indicated genotypes hybridized with ( C ) a <t>DIG-labeled</t> 5′ fragment from the KLP38B cDNA or ( D ) an antisense riboprobe generated from the cDNA representing the 1.8-kb transcript. Note that in C , instead of the wild-type 3.6-kb transcript, a truncated 1.6-kb transcript appeared in tio 1 homo- or hemizygotes. The transcript returned to normal size in larvae homo- or hemizygous for tio tr1 , a transposase-induced true revertant of tio 1 . In contrast, no change was observed in the 1.8-kb transcript in tio 1 animals ( D ).
    Dig Dna Labeling Kit, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dig dna labeling kit/product/Boehringer Mannheim
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dig dna labeling kit - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    86
    Boehringer Mannheim random primed dna dig labelling kit
    Confirmation of gene disruption by Southern analysis. Lane 1, <t>DNA</t> from the control strain; lane 2, DNA from the disrupted strain; lane 3, Dig-labelled DNA molecular weight marker III (Boehringer <t>Mannheim).</t>
    Random Primed Dna Dig Labelling Kit, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/random primed dna dig labelling kit/product/Boehringer Mannheim
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    random primed dna dig labelling kit - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    Image Search Results


    Molecular organization of the tio gene. ( A ) Top line: Restriction map of the 20 kb of genomic DNA containing tio . Lower lines: Exon/intron map of the 3.6-kb KLP38B transcript and the 1.8-kb transcript contained within its large intron. The tio 1 P element is inserted within the intron of the 3.6-kb transcript, in a 700-bp PvuII-HindIII fragment upstream of the 5′ end of the cDNA representing the 1.8-kb transcript. The tio 24-0 (*) and tio 93-E (**) inserts were mapped to the intron of the 3.6-kb transcript by Alphey et al. ( 3 ), roughly as shown by the asterisks. The exact relationship of the tio 24-0 and tio 93-E inserts to the position of the intronic transcript was not published in Alphey et al. ( 3 ). ( B ) Developmental Northern probed with a cloned genomic fragment showing the two transcripts (3.6 and 1.8 kb) derived from the genomic DNA flanking the tio 1 P element insert. Both transcripts were present in all stages studied, although the 3.6-kb transcript was more abundant in 0–2-h embryos and the 1.8-kb transcript was more abundant in 4–8-h embryos. ( C and D ) Northern analysis of polyA + RNA from third instar larvae of the indicated genotypes hybridized with ( C ) a DIG-labeled 5′ fragment from the KLP38B cDNA or ( D ) an antisense riboprobe generated from the cDNA representing the 1.8-kb transcript. Note that in C , instead of the wild-type 3.6-kb transcript, a truncated 1.6-kb transcript appeared in tio 1 homo- or hemizygotes. The transcript returned to normal size in larvae homo- or hemizygous for tio tr1 , a transposase-induced true revertant of tio 1 . In contrast, no change was observed in the 1.8-kb transcript in tio 1 animals ( D ).

    Journal: The Journal of Cell Biology

    Article Title: A Chromatin-associated Kinesin-related Protein Required for Normal Mitotic Chromosome Segregation in Drosophila

    doi:

    Figure Lengend Snippet: Molecular organization of the tio gene. ( A ) Top line: Restriction map of the 20 kb of genomic DNA containing tio . Lower lines: Exon/intron map of the 3.6-kb KLP38B transcript and the 1.8-kb transcript contained within its large intron. The tio 1 P element is inserted within the intron of the 3.6-kb transcript, in a 700-bp PvuII-HindIII fragment upstream of the 5′ end of the cDNA representing the 1.8-kb transcript. The tio 24-0 (*) and tio 93-E (**) inserts were mapped to the intron of the 3.6-kb transcript by Alphey et al. ( 3 ), roughly as shown by the asterisks. The exact relationship of the tio 24-0 and tio 93-E inserts to the position of the intronic transcript was not published in Alphey et al. ( 3 ). ( B ) Developmental Northern probed with a cloned genomic fragment showing the two transcripts (3.6 and 1.8 kb) derived from the genomic DNA flanking the tio 1 P element insert. Both transcripts were present in all stages studied, although the 3.6-kb transcript was more abundant in 0–2-h embryos and the 1.8-kb transcript was more abundant in 4–8-h embryos. ( C and D ) Northern analysis of polyA + RNA from third instar larvae of the indicated genotypes hybridized with ( C ) a DIG-labeled 5′ fragment from the KLP38B cDNA or ( D ) an antisense riboprobe generated from the cDNA representing the 1.8-kb transcript. Note that in C , instead of the wild-type 3.6-kb transcript, a truncated 1.6-kb transcript appeared in tio 1 homo- or hemizygotes. The transcript returned to normal size in larvae homo- or hemizygous for tio tr1 , a transposase-induced true revertant of tio 1 . In contrast, no change was observed in the 1.8-kb transcript in tio 1 animals ( D ).

    Article Snippet: DNA probes were either digoxigenin-labeled by the random primed method using the DIG-DNA labeling kit ( Boehringer Mannheim Corp. ) or radioactively labeled by random priming.

    Techniques: Northern Blot, Clone Assay, Derivative Assay, Labeling, Generated

    Confirmation of gene disruption by Southern analysis. Lane 1, DNA from the control strain; lane 2, DNA from the disrupted strain; lane 3, Dig-labelled DNA molecular weight marker III (Boehringer Mannheim).

    Journal: Applied and Environmental Microbiology

    Article Title: Isolation, Nucleotide Sequence, and Physiological Relevance of the Gene Encoding Triose Phosphate Isomerase from Kluyveromyces lactis

    doi:

    Figure Lengend Snippet: Confirmation of gene disruption by Southern analysis. Lane 1, DNA from the control strain; lane 2, DNA from the disrupted strain; lane 3, Dig-labelled DNA molecular weight marker III (Boehringer Mannheim).

    Article Snippet: Hybridization was performed by using as a probe the Bgl II- Csp 45 I fragment, Dig-labelled (Random Primed DNA Dig-labelling kit; Boehringer Mannheim) at 48°C in 50% formamide, 5× SSC, 0.2% SDS, and 2.5% blocking solution.

    Techniques: Molecular Weight, Marker