rabbit tdp 43 c terminal polyclonal  (Proteintech)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 98
    Name:
    Rabbit TDP 43 C terminal Polyclonal
    Description:
    The TDP 43 C terminal antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human TDP 43 C terminal This antibody recognizes human mouse rat antigen The TDP 43 C terminal antibody has been validated for the following applications ELISA IF IHC IP WB analysis
    Catalog Number:
    12892-1-AP
    Price:
    [321.93]
    Applications:
    IHC,Western Blot,ChIP,ELISA,Immunoprecipitation,Immunofluorescence
    Host:
    Rabbit
    Conjugate:
    Unconjugated
    Immunogen:
    Recombinant Protein
    Size:
    150 ul
    Category:
    Antibody
    Antibody Type:
    Primary antibody
    Isotype:
    IgG
    Reactivity:
    Human Mouse Rat Zebrafish Monkey
    Buy from Supplier


    Structured Review

    Proteintech rabbit tdp 43 c terminal polyclonal
    Immunohistochemistry of mouse testis with <t>anti-TDP-43</t> <t>polyclonal</t> antibody GP#2. Each panel corresponds to seminiferous tubule Stages I–XII (a–l, respectively). Scale bar, 30 μm. A 3 and A 4 , differentiating spermatogonia; ES, elongated spermatozoa; In, intermediate spermatogonia; L, leptotene; and MII, spermatocytes undergoing second meiotic division; P, pachytene spermatocytes; PL, preleptotene spermatocytes; R, round spermatids, RB, residual body; Z, zygotene.
    The TDP 43 C terminal antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human TDP 43 C terminal This antibody recognizes human mouse rat antigen The TDP 43 C terminal antibody has been validated for the following applications ELISA IF IHC IP WB analysis
    https://www.bioz.com/result/rabbit tdp 43 c terminal polyclonal/product/Proteintech
    Average 98 stars, based on 27 article reviews
    Price from $9.99 to $1999.99
    rabbit tdp 43 c terminal polyclonal - by Bioz Stars, 2020-09
    98/100 stars

    Images

    1) Product Images from "Immunolocalization of TAR DNA-Binding Protein of 43 kDa (TDP-43) in mouse seminiferous epithelium"

    Article Title: Immunolocalization of TAR DNA-Binding Protein of 43 kDa (TDP-43) in mouse seminiferous epithelium

    Journal: Molecular reproduction and development

    doi: 10.1002/mrd.22851

    Immunohistochemistry of mouse testis with anti-TDP-43 polyclonal antibody GP#2. Each panel corresponds to seminiferous tubule Stages I–XII (a–l, respectively). Scale bar, 30 μm. A 3 and A 4 , differentiating spermatogonia; ES, elongated spermatozoa; In, intermediate spermatogonia; L, leptotene; and MII, spermatocytes undergoing second meiotic division; P, pachytene spermatocytes; PL, preleptotene spermatocytes; R, round spermatids, RB, residual body; Z, zygotene.
    Figure Legend Snippet: Immunohistochemistry of mouse testis with anti-TDP-43 polyclonal antibody GP#2. Each panel corresponds to seminiferous tubule Stages I–XII (a–l, respectively). Scale bar, 30 μm. A 3 and A 4 , differentiating spermatogonia; ES, elongated spermatozoa; In, intermediate spermatogonia; L, leptotene; and MII, spermatocytes undergoing second meiotic division; P, pachytene spermatocytes; PL, preleptotene spermatocytes; R, round spermatids, RB, residual body; Z, zygotene.

    Techniques Used: Immunohistochemistry

    Immunohistochemistry of mouse testis with anti TDP-43 polyclonal GP#3 or Abcam antibodies. Sections stained with (a–d) GP#3 (recognizes amino acids 3–271 of TDP-43) or (e–h) Abcam antibody (recognizes amino acids 250–350 of TDP-43). Stages are indicated with Roman numerals. Scale bar, 30 μm. A 3 and A 4 , differentiating spermatogonia; ES, elongated spermatozoa; In, intermediate spermatogonia; L, leptotene; and MII, spermatocytes undergoing second meiotic division; P, pachytene spermatocytes; PL, preleptotene spermatocytes; R, round spermatids, RB, residual body; st9, step 9 spermatid; st10, step 10 spermatid; Z, zygotene.
    Figure Legend Snippet: Immunohistochemistry of mouse testis with anti TDP-43 polyclonal GP#3 or Abcam antibodies. Sections stained with (a–d) GP#3 (recognizes amino acids 3–271 of TDP-43) or (e–h) Abcam antibody (recognizes amino acids 250–350 of TDP-43). Stages are indicated with Roman numerals. Scale bar, 30 μm. A 3 and A 4 , differentiating spermatogonia; ES, elongated spermatozoa; In, intermediate spermatogonia; L, leptotene; and MII, spermatocytes undergoing second meiotic division; P, pachytene spermatocytes; PL, preleptotene spermatocytes; R, round spermatids, RB, residual body; st9, step 9 spermatid; st10, step 10 spermatid; Z, zygotene.

    Techniques Used: Immunohistochemistry, Staining

    Characterization of polyclonal antibodies GP#2 and GP#3, generated against the amino terminus of TDP-43. Two (lane 1) or one microgram (lane 3) of GST-tagged full-length TDP-43, or 2 μg each of GST-Δgly TDP-43 (amino acids 3–271) (lane 2); His-tagged PURα (lane 4); His-tagged SP-10 (lane 5); and His-tagged Δgly (amino acids 3–271) (lane 6) were separated in duplicate gels, blotted, and probed with GP#2 (a) or GP#3 (b).
    Figure Legend Snippet: Characterization of polyclonal antibodies GP#2 and GP#3, generated against the amino terminus of TDP-43. Two (lane 1) or one microgram (lane 3) of GST-tagged full-length TDP-43, or 2 μg each of GST-Δgly TDP-43 (amino acids 3–271) (lane 2); His-tagged PURα (lane 4); His-tagged SP-10 (lane 5); and His-tagged Δgly (amino acids 3–271) (lane 6) were separated in duplicate gels, blotted, and probed with GP#2 (a) or GP#3 (b).

    Techniques Used: Generated

    2) Product Images from "Isolation and characterization of antibody fragments selective for human FTD brain derived TDP-43 variants"

    Article Title: Isolation and characterization of antibody fragments selective for human FTD brain derived TDP-43 variants

    Journal: BMC Neuroscience

    doi: 10.1186/s12868-020-00586-0

    Western Blot Analysis. Reactivity against healthy control tissue and TDP-43 immunoprecipitated from healthy controls and FTD was assessed under non-reducing and non-denaturing conditions with ( a ) Commercial TDP antibody identifying TDP variants in FTD and healthy controls, and ( b ) FTD-TDP2 scFv which recognizes disease variant of TDP (~ 70 kDa) present in FTD and not healthy controls
    Figure Legend Snippet: Western Blot Analysis. Reactivity against healthy control tissue and TDP-43 immunoprecipitated from healthy controls and FTD was assessed under non-reducing and non-denaturing conditions with ( a ) Commercial TDP antibody identifying TDP variants in FTD and healthy controls, and ( b ) FTD-TDP2 scFv which recognizes disease variant of TDP (~ 70 kDa) present in FTD and not healthy controls

    Techniques Used: Western Blot, Immunoprecipitation, Variant Assay

    Related Articles

    Staining:

    Article Title: Astrocytes with TDP-43 inclusions exhibit reduced noradrenergic cAMP and Ca2+ signaling and dysregulated cell metabolism
    Article Snippet: .. Astrocytes were then stained with primary antibodies against endogenous TDP-43 (C-terminal, rabbit polyclonal, 1:400; Proteintech, Manchester, UK), α1 -AR (rabbit polyclonal, 1:100; Abcam, Cambridge, UK), β1 -AR (rabbit polyclonal, 1:200; Abcam, Cambridge, UK), β2 -AR (rabbit polyclonal, 1:100; Biorbyt, Cambridge, UK), MCT1 (rabbit polyclonal, 1:50; Abcam, Cambridge, UK) and MCT4 (rabbit polyclonal, 1:50; Santa Cruz, Texas, US) overnight at 4 °C. .. After washing with PBS, cells were incubated for 1 h at 37 °C with Alexa Fluor488 -conjugated secondary goat anti-rabbit IgG (1:600; Abcam, Cambridge, UK).

    Incubation:

    Article Title: Heavy Metal Neurotoxicants Induce ALS-Linked TDP-43 Pathology
    Article Snippet: .. Blots were incubated with the following antibodies: 1:1000 anti-phospho-S409/410 TDP-43 (a gift from Leonard Petrucelli, Mayo Clinic, Rb3655), 1:2000 anti-GFP (Sigma; G1544), 1:2000 anti-TDP-43 (ProteinTech; 12892–1-AP); and 1:10000 anti-Actin (Millipore; MAB1501). .. Blots were imaged using HRP-conjugated secondary antibodies (Jackson) activated with Pierce ECL chemiluminescent substrates (Thermo Fisher) and a ChemiDoc XRS+ Imager (BioRad).

    Article Title: Immunolocalization of TAR DNA-Binding Protein of 43 kDa (TDP-43) in mouse seminiferous epithelium
    Article Snippet: .. Membranes were then blocked at room temperature for 1 h in 3% bovine serum albumin, and incubated at 4°C overnight with primary antibodies ( ) using the following dilutions: Proteintech anti-TDP-43 (1:1200 dilution of 12892-1-AP) (Proteintech, IL, USA), Abcam anti-TDP-43 (1:3000 dilution of ab41972), GP#2 and #3 anti-TDP-43 (1:8000 dilution of each) (see Section 4.2), or mouse monoclonal anti-tubulin (1: 5000 dilution of T0198) (Sigma-Aldrich, MO, USA). .. Membranes were then incubated for 1 h at room temperature with appropriate horseradish peroxidase-conjugated secondary antibodies (1:15000) (Santa Cruz Biotechnology, CA, USA).

    Immunofluorescence:

    Article Title: Progranulin-mediated deficiency of cathepsin D results in FTD and NCL-like phenotypes in neurons derived from FTD patients
    Article Snippet: .. The antibodies used in this study were: TDP-43 (Protein Tech, 12892–1-AP), Vinculin (Abcam, ab18058), LAMP1 (DSHB Hybridoma, H4A3-s) (for Western blots), LAMP1 (Santa Cruz, sc-20011) (for immunofluorescence), LAMP2 (DSHB Hybridoma, H4B4-s), Cathepsin D (Abcam, ab75852), cathepsin B (R & D, AF953) (for Western blots), cathepsin B (Abcam, ab125067) (for co-immunoprecipitation assay), GAPDH (Millipore, MAB374), B-III tubulin (Biolegend, 801201), MAP2 (Sigma, M4403), PGRN (Abcam, ab108608), GRN E (Anti-granulin C-terminal) (Sigma, SAB4200310), NSE (ThermoFisher, PA5–12374), VGLUT1/2 (Synaptic Systems, 135303), Brn2 (Cell Signaling, 12137) and CUX1 (Santa Cruz, sc-13024). .. All antibodies were used at a 1: 1000 dilution for Western blot analysis (in 5% Bovine Serum Albumin, 0.5% Sodium Azide) and a 1: 200 dilution for immunofluorescence analysis (in 5% normal goat serum, 1% BSA).

    Co-Immunoprecipitation Assay:

    Article Title: Progranulin-mediated deficiency of cathepsin D results in FTD and NCL-like phenotypes in neurons derived from FTD patients
    Article Snippet: .. The antibodies used in this study were: TDP-43 (Protein Tech, 12892–1-AP), Vinculin (Abcam, ab18058), LAMP1 (DSHB Hybridoma, H4A3-s) (for Western blots), LAMP1 (Santa Cruz, sc-20011) (for immunofluorescence), LAMP2 (DSHB Hybridoma, H4B4-s), Cathepsin D (Abcam, ab75852), cathepsin B (R & D, AF953) (for Western blots), cathepsin B (Abcam, ab125067) (for co-immunoprecipitation assay), GAPDH (Millipore, MAB374), B-III tubulin (Biolegend, 801201), MAP2 (Sigma, M4403), PGRN (Abcam, ab108608), GRN E (Anti-granulin C-terminal) (Sigma, SAB4200310), NSE (ThermoFisher, PA5–12374), VGLUT1/2 (Synaptic Systems, 135303), Brn2 (Cell Signaling, 12137) and CUX1 (Santa Cruz, sc-13024). .. All antibodies were used at a 1: 1000 dilution for Western blot analysis (in 5% Bovine Serum Albumin, 0.5% Sodium Azide) and a 1: 200 dilution for immunofluorescence analysis (in 5% normal goat serum, 1% BSA).

    Western Blot:

    Article Title: Progranulin-mediated deficiency of cathepsin D results in FTD and NCL-like phenotypes in neurons derived from FTD patients
    Article Snippet: .. The antibodies used in this study were: TDP-43 (Protein Tech, 12892–1-AP), Vinculin (Abcam, ab18058), LAMP1 (DSHB Hybridoma, H4A3-s) (for Western blots), LAMP1 (Santa Cruz, sc-20011) (for immunofluorescence), LAMP2 (DSHB Hybridoma, H4B4-s), Cathepsin D (Abcam, ab75852), cathepsin B (R & D, AF953) (for Western blots), cathepsin B (Abcam, ab125067) (for co-immunoprecipitation assay), GAPDH (Millipore, MAB374), B-III tubulin (Biolegend, 801201), MAP2 (Sigma, M4403), PGRN (Abcam, ab108608), GRN E (Anti-granulin C-terminal) (Sigma, SAB4200310), NSE (ThermoFisher, PA5–12374), VGLUT1/2 (Synaptic Systems, 135303), Brn2 (Cell Signaling, 12137) and CUX1 (Santa Cruz, sc-13024). .. All antibodies were used at a 1: 1000 dilution for Western blot analysis (in 5% Bovine Serum Albumin, 0.5% Sodium Azide) and a 1: 200 dilution for immunofluorescence analysis (in 5% normal goat serum, 1% BSA).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 98
    Proteintech rabbit tdp 43 c terminal polyclonal
    SQSTM1 mutation SQSTM1 G427R enhances <t>TARDBP-associated</t> stress granule formation upon oxidative stress. (A) MEFs sqstm1 KO stably expressing Flag-SQSTM1 WT or SQSTM1 G427R mutant were treated with AS (10 μM for 6 h), followed by immunofluorescent staining with anti-TARDBP (red) and anti-G3BP1 (green) antibodies and visualizing with fluorescent microscope. Scale bar: 10 μm. Arrows indicate cytosol TARDBP foci co-localized to stress granule marker G3BP1. (B) Quantification of the results in A was performed by counting the number of cells containing TARDBP-positive stress granules as the fraction of the total number of cells. Student t -test was used, and values are presented as the mean ± SEM (n = 3). ** p
    Rabbit Tdp 43 C Terminal Polyclonal, supplied by Proteintech, used in various techniques. Bioz Stars score: 98/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit tdp 43 c terminal polyclonal/product/Proteintech
    Average 98 stars, based on 27 article reviews
    Price from $9.99 to $1999.99
    rabbit tdp 43 c terminal polyclonal - by Bioz Stars, 2020-09
    98/100 stars
      Buy from Supplier

    Image Search Results


    SQSTM1 mutation SQSTM1 G427R enhances TARDBP-associated stress granule formation upon oxidative stress. (A) MEFs sqstm1 KO stably expressing Flag-SQSTM1 WT or SQSTM1 G427R mutant were treated with AS (10 μM for 6 h), followed by immunofluorescent staining with anti-TARDBP (red) and anti-G3BP1 (green) antibodies and visualizing with fluorescent microscope. Scale bar: 10 μm. Arrows indicate cytosol TARDBP foci co-localized to stress granule marker G3BP1. (B) Quantification of the results in A was performed by counting the number of cells containing TARDBP-positive stress granules as the fraction of the total number of cells. Student t -test was used, and values are presented as the mean ± SEM (n = 3). ** p

    Journal: Autophagy

    Article Title: ALS-FTLD-linked mutations of SQSTM1/p62 disrupt selective autophagy and NFE2L2/NRF2 anti-oxidative stress pathway

    doi: 10.1080/15548627.2019.1644076

    Figure Lengend Snippet: SQSTM1 mutation SQSTM1 G427R enhances TARDBP-associated stress granule formation upon oxidative stress. (A) MEFs sqstm1 KO stably expressing Flag-SQSTM1 WT or SQSTM1 G427R mutant were treated with AS (10 μM for 6 h), followed by immunofluorescent staining with anti-TARDBP (red) and anti-G3BP1 (green) antibodies and visualizing with fluorescent microscope. Scale bar: 10 μm. Arrows indicate cytosol TARDBP foci co-localized to stress granule marker G3BP1. (B) Quantification of the results in A was performed by counting the number of cells containing TARDBP-positive stress granules as the fraction of the total number of cells. Student t -test was used, and values are presented as the mean ± SEM (n = 3). ** p

    Article Snippet: AntibodiesThe following antibodies were used: SQSTM1 (Progen Biotechnik, GP62-C-WBC), p-SQSTM1 Ser403 (Millipore, MABC186-I), p-SQSTM1 Ser409 (constructed by our lab), p-SQSTM1 Ser351 (MBL, PM074), ULK1 (Sigma-Aldrich, A7481), Flag-M2 (Sigma-Aldrich, F1804), anti-ACTB/β-Actin (Cell Signaling Technology, 3700), MYC (Cell Signaling Technology, 2278), Ubiquitin (Dako, Z0458), GFP (Thermo Fisher Scientific, A11122), TBK1 (Abcam, ab40676), p-TBK1 Ser172 (Cell Signaling Technology, 5483), SOD1 (Santa Cruz Biotechnology, SC11407), KEAP1 (Proteintech, Cosmo Bio, 10503-2-AP), CALCOCO2/NDP52 (Proteintech, Cosmo Bio, 12229-1-AP), NBR1 (Proteintech, Cosmo Bio, 16004-1-AP), NQO1 (Proteintech, Cosmo Bio, 11451-1-AP), HMOX1/HO-1 (Santa Cruz Biotechnology, sc-10789), GSTM1 (Proteintech, Cosmo Bio, 12412-1-AP), TARDBP (Proteintech Cosmo Bio, 12892-1-AP), G3BP1 (Sigma-Aldrich, HPA004052), LC3B (MBL, PM036) and MAP2 (Sigma-Aldrich, M4403).

    Techniques: Mutagenesis, Stable Transfection, Expressing, Staining, Microscopy, Marker

    Immunohistochemistry of mouse testis with anti-TDP-43 polyclonal antibody GP#2. Each panel corresponds to seminiferous tubule Stages I–XII (a–l, respectively). Scale bar, 30 μm. A 3 and A 4 , differentiating spermatogonia; ES, elongated spermatozoa; In, intermediate spermatogonia; L, leptotene; and MII, spermatocytes undergoing second meiotic division; P, pachytene spermatocytes; PL, preleptotene spermatocytes; R, round spermatids, RB, residual body; Z, zygotene.

    Journal: Molecular reproduction and development

    Article Title: Immunolocalization of TAR DNA-Binding Protein of 43 kDa (TDP-43) in mouse seminiferous epithelium

    doi: 10.1002/mrd.22851

    Figure Lengend Snippet: Immunohistochemistry of mouse testis with anti-TDP-43 polyclonal antibody GP#2. Each panel corresponds to seminiferous tubule Stages I–XII (a–l, respectively). Scale bar, 30 μm. A 3 and A 4 , differentiating spermatogonia; ES, elongated spermatozoa; In, intermediate spermatogonia; L, leptotene; and MII, spermatocytes undergoing second meiotic division; P, pachytene spermatocytes; PL, preleptotene spermatocytes; R, round spermatids, RB, residual body; Z, zygotene.

    Article Snippet: Membranes were then blocked at room temperature for 1 h in 3% bovine serum albumin, and incubated at 4°C overnight with primary antibodies ( ) using the following dilutions: Proteintech anti-TDP-43 (1:1200 dilution of 12892-1-AP) (Proteintech, IL, USA), Abcam anti-TDP-43 (1:3000 dilution of ab41972), GP#2 and #3 anti-TDP-43 (1:8000 dilution of each) (see Section 4.2), or mouse monoclonal anti-tubulin (1: 5000 dilution of T0198) (Sigma-Aldrich, MO, USA).

    Techniques: Immunohistochemistry

    Immunohistochemistry of mouse testis with anti TDP-43 polyclonal GP#3 or Abcam antibodies. Sections stained with (a–d) GP#3 (recognizes amino acids 3–271 of TDP-43) or (e–h) Abcam antibody (recognizes amino acids 250–350 of TDP-43). Stages are indicated with Roman numerals. Scale bar, 30 μm. A 3 and A 4 , differentiating spermatogonia; ES, elongated spermatozoa; In, intermediate spermatogonia; L, leptotene; and MII, spermatocytes undergoing second meiotic division; P, pachytene spermatocytes; PL, preleptotene spermatocytes; R, round spermatids, RB, residual body; st9, step 9 spermatid; st10, step 10 spermatid; Z, zygotene.

    Journal: Molecular reproduction and development

    Article Title: Immunolocalization of TAR DNA-Binding Protein of 43 kDa (TDP-43) in mouse seminiferous epithelium

    doi: 10.1002/mrd.22851

    Figure Lengend Snippet: Immunohistochemistry of mouse testis with anti TDP-43 polyclonal GP#3 or Abcam antibodies. Sections stained with (a–d) GP#3 (recognizes amino acids 3–271 of TDP-43) or (e–h) Abcam antibody (recognizes amino acids 250–350 of TDP-43). Stages are indicated with Roman numerals. Scale bar, 30 μm. A 3 and A 4 , differentiating spermatogonia; ES, elongated spermatozoa; In, intermediate spermatogonia; L, leptotene; and MII, spermatocytes undergoing second meiotic division; P, pachytene spermatocytes; PL, preleptotene spermatocytes; R, round spermatids, RB, residual body; st9, step 9 spermatid; st10, step 10 spermatid; Z, zygotene.

    Article Snippet: Membranes were then blocked at room temperature for 1 h in 3% bovine serum albumin, and incubated at 4°C overnight with primary antibodies ( ) using the following dilutions: Proteintech anti-TDP-43 (1:1200 dilution of 12892-1-AP) (Proteintech, IL, USA), Abcam anti-TDP-43 (1:3000 dilution of ab41972), GP#2 and #3 anti-TDP-43 (1:8000 dilution of each) (see Section 4.2), or mouse monoclonal anti-tubulin (1: 5000 dilution of T0198) (Sigma-Aldrich, MO, USA).

    Techniques: Immunohistochemistry, Staining

    Characterization of polyclonal antibodies GP#2 and GP#3, generated against the amino terminus of TDP-43. Two (lane 1) or one microgram (lane 3) of GST-tagged full-length TDP-43, or 2 μg each of GST-Δgly TDP-43 (amino acids 3–271) (lane 2); His-tagged PURα (lane 4); His-tagged SP-10 (lane 5); and His-tagged Δgly (amino acids 3–271) (lane 6) were separated in duplicate gels, blotted, and probed with GP#2 (a) or GP#3 (b).

    Journal: Molecular reproduction and development

    Article Title: Immunolocalization of TAR DNA-Binding Protein of 43 kDa (TDP-43) in mouse seminiferous epithelium

    doi: 10.1002/mrd.22851

    Figure Lengend Snippet: Characterization of polyclonal antibodies GP#2 and GP#3, generated against the amino terminus of TDP-43. Two (lane 1) or one microgram (lane 3) of GST-tagged full-length TDP-43, or 2 μg each of GST-Δgly TDP-43 (amino acids 3–271) (lane 2); His-tagged PURα (lane 4); His-tagged SP-10 (lane 5); and His-tagged Δgly (amino acids 3–271) (lane 6) were separated in duplicate gels, blotted, and probed with GP#2 (a) or GP#3 (b).

    Article Snippet: Membranes were then blocked at room temperature for 1 h in 3% bovine serum albumin, and incubated at 4°C overnight with primary antibodies ( ) using the following dilutions: Proteintech anti-TDP-43 (1:1200 dilution of 12892-1-AP) (Proteintech, IL, USA), Abcam anti-TDP-43 (1:3000 dilution of ab41972), GP#2 and #3 anti-TDP-43 (1:8000 dilution of each) (see Section 4.2), or mouse monoclonal anti-tubulin (1: 5000 dilution of T0198) (Sigma-Aldrich, MO, USA).

    Techniques: Generated

    TDP-43 and other disease-linked RNA-binding proteins form nuclear inclusions in GA-Nes mice. a Neuronal nuclear inclusions of TDP-43 (arrow) are detected by immunofluorescence in hippocampus and frontotemporal cortex in GA-Nes end-stage mice, but not Nes littermate controls. No TDP-43 inclusions are detected in affected or asymptomatic PR-Nes mice, or a 68-week Nes control, even in cells with abundant poly-PR (green) inclusions. Scale bar = 10 µm. b Schematic drawing of the distribution of the rare nuclear TDP-43 aggregates in GA-Nes mice. Aggregates were found in the lateral frontal cortex (at least 100 µm from the midline) and hippocampal CA regions marked with red x . c Regional overview of TDP-43 nuclear aggregates (arrows) in the frontal cortex and hippocampus CA3 region of end-stage GA-Nes mice, scale bar = 100 µm. d Nuclear TDP-43 aggregates (arrows) in end-stage GA-Nes mice colocalize with hnRNP H, and weakly with hnRNP A1, but not with Fus, Sfpq or Alyref. No nuclear aggregates of any marker are seen in age-matched Nes mice. Scale bar = 10 µm

    Journal: Acta Neuropathologica

    Article Title: Congenic expression of poly-GA but not poly-PR in mice triggers selective neuron loss and interferon responses found in C9orf72 ALS

    doi: 10.1007/s00401-020-02176-0

    Figure Lengend Snippet: TDP-43 and other disease-linked RNA-binding proteins form nuclear inclusions in GA-Nes mice. a Neuronal nuclear inclusions of TDP-43 (arrow) are detected by immunofluorescence in hippocampus and frontotemporal cortex in GA-Nes end-stage mice, but not Nes littermate controls. No TDP-43 inclusions are detected in affected or asymptomatic PR-Nes mice, or a 68-week Nes control, even in cells with abundant poly-PR (green) inclusions. Scale bar = 10 µm. b Schematic drawing of the distribution of the rare nuclear TDP-43 aggregates in GA-Nes mice. Aggregates were found in the lateral frontal cortex (at least 100 µm from the midline) and hippocampal CA regions marked with red x . c Regional overview of TDP-43 nuclear aggregates (arrows) in the frontal cortex and hippocampus CA3 region of end-stage GA-Nes mice, scale bar = 100 µm. d Nuclear TDP-43 aggregates (arrows) in end-stage GA-Nes mice colocalize with hnRNP H, and weakly with hnRNP A1, but not with Fus, Sfpq or Alyref. No nuclear aggregates of any marker are seen in age-matched Nes mice. Scale bar = 10 µm

    Article Snippet: Immunohistochemistry and immunofluorescenceImmunohistochemistry and immunofluorescence was performed on 5-µm paraffin-embedded tissue sections as described previously [ ] using the following antibodies: Alyref (ab6141, Abcam 1:50), ChAT (AB144P, Millipore 1:200), GFP (632381, Takara 1:250), Fus (A300-292A, Bethyl Labs 1:100), Hnrnpa1 (sc-32301, Santa Cruz 1:50), Hnrnph (A300-511A, Bethyl Labs 1:500), Iba1 (091-19741, Wako, 1:500 and ab5076, Abcam, 1:500), Map2 (M1406, Sigma 1:250), NeuN (ab177487, Abcam, 1:1000), nucleolin (ab50279, Abcam 1:30), p62 (PM045, MBL 1:1000), poly-GA (1A12, mouse, purified and biotinylated, 1:500) [ ], poly-PR (32B3, mouse purified and biotinylated, Helmholtz Zentrum 1:1000) [ ], Psmc4 (A303-850A, Bethyl Labs 1:250), Alexa Fluor 594 anti-Sfpq (A301-321A, Bethyl Labs 1:100), TDP-43N-terminal (10782-2-AP, Proteintech 1:500), TDP-43 p409/410 (1D3, gift from Manuela Neumann 1:500), TDP-43 p409/410 (CAC-TIP-PTD-P02, Cosmo 1:500), TDP-43 p409/410 (CAC-TIP-PTD-M01, Cosmo 1:1000), TDP43 p409/410 (3655, gift from Leonard Petrucelli 1:500), TDP-43 pS403/404 (CAC-TIP-PTD-P05, Cosmo 1:500), TDP-43 pT153/Y155 (gift from Yuna Ayala 1:300), TDP-43 C-terminal (12892-1-AP, Proteintech 1:100), TDP-43 non-phospho 404–413 (TAR5 6D6, gift from Manuela Neumann 1:5), TDP-43 non-phospho 404–413 (TAR5 2H4, gift from Manuela Neumann 1:5), TDP-43 N-terminal (10782-2-AP, Proteintech 1:500), TDP-43 p409/410 (1D3, gift from Manuela Neumann 1:500), TDP-43 p409/410 (CAC-TIP-PTD-P02, Cosmo 1:500), TDP-43 p409/410 (CAC-TIP-PTD-M01, Cosmo 1:1000), TDP43 p409/410 (3655, gift from Leonard Petrucelli 1:500), TDP-43 pS403/404 (CAC-TIP-PTD-P05, Cosmo 1:500), TDP-43 pT153/Y155 (gift from Yuna Ayala 1:300), TDP-43 C-terminal (12892-1-AP, Proteintech 1:100), TDP-43 non-phospho 404–413 (TAR5 6D6, gift from Manuela Neumann 1:5), TDP-43 non-phospho 404–413 (TAR5 2H4, gift from Manuela Neumann 1:5), Tubulin β 3 (801208, BioLegend 1:200).

    Techniques: RNA Binding Assay, Mouse Assay, Immunofluorescence, Marker